Spo22735.1 (mRNA) | SpinachBase

Overview


Name	Spo22735.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Mitochondrial import inner membrane translocase subunit TIM14-1 (Chaperone DnaJ-domain containing protein 1)
Location	SpoScf_01588 : 116613 .. 120274 (-)
Sequence length	947

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACATGACCCAGAAAGAGATTTCTGAAGGGTTTTCGATAATTGGCCATCAAAGTGTTTTCATTGAACTTTCTAGCACATACTCGAAACTTCACGAGTAATCACGACGGAAACAAAGAACATCATTTCAAGCCAACTCTGAAGACAAATCCAAAGCTGAATTTCTTTGTCATAAAATCGTCATAAAAATGGTCAGTTGCCCCTCTTCATTCACCCCTTCTTTTGGTTTTAATATTGTTCTTGTTTTTCTCACACTTTGAATTGTTCTCAATTTTTATGAATTATGATTGATAATTTTGCAAATGCTTGCTTAATTGTTGTGATATTGCATTAAAGTTATGAAAATTTCGTGAATTCAACTTCTGTAGAAGATTTGTGGCTGTTTTGGGTAAATTTTAACCTTTTGTTGCTTCGTTAAATCGGGTTTTTGTCTAAGATGTTTTTAATTTTATTTGGGGGAGATTAGGGTTTGAGTGATTTATTTTTTTATAATTACAGAAAATATTGAACGGGTCTATTTTTATTTTCTTGTTTTGGATATATCTGCTAGGGAATAAATCAGTGGTTTCTTTAGAGGATCCAATCGGTGTTAATTTGATCTGTTTTTTGCCAAATGATGAATTTCCCTTCCCATTATGTATTAAATTCGGATTAATGAGTTGTTTGTCTGCAATTCATGTGTAGTTTCATGAGAAAATTATGTGATTGCAATTTTGGTATTATGAATAATAAGCTAAATCTATAAACTGGGGATTTCTTGTTGAACTGGTTCAAATTTGAGGTGAATTGTGAGCTTCTGCTCAATAGCATCAAGCAGACAAGCACAATCTCCGCCTCACCACTACCATAAGGTAGTGAGGGGTGTGCCACATTCGGCTTGGGCATCCTTCTGTAAGGGTGGCTTGGTGGGAACTGTAAGGGTGGCTTGGTGGGAACTGTAAGGGTGGCTTGGTGGGGGATACGGACATCAGTGGGTTATGGACTCAGATTGGCCTTTGAATGTCCCACATATGTTGATACAACTATACCAGTCTATAACCAATCCCTAAAAATATGTGGGAGAGGAAGAGAGAGAGGGCATGTCTTCTGTAAGGCAATAGAGGTAGTTGGCTTGTACGAATGCAGCGGCTTTTTTTCCATTTTAATTCATCTTTTGGAGCAACCCTGTGCTTGTTTTGAGTAGCCGAGTACATTTTGAGGTGATTGGTTCATTGTAAAGAAACCAAAAACTTTGATCTCGAAGTATATATTGCATGAAGTGCTTTTGGATAAAGTTGTTTATTGTTGGTACTTGATCACATATTGTTATCAGTCTATGATTGCGAAACATCTTTGTGTTAAATTTCTTATCTTGTGATGCAAATGATAATTTTTACTTGAAAACCATGTCGACGAAATAGAGTGTTGATTATATTGTATAAGTGATCCTAAAATATAACAGTTTTCATTGCTAGATGGAAGTAAGGATTGTTTTGATCTATAATCTAGTTATATCAGTTTTCACATGTAATGTTTCCCTTTTTCGTTCTTTAGTTATGTCATTAGCATTTTGATTCAAAATTTTACCCTCATATGTGAAGAGTCCAAGGATGCACCAAATTTGCTGTATTTTATGTATGTTTTTATGTTTGAACAGTTTAAAGATACTTGCATTGTAATGTGGACCTTTCCTGCATTTGATTGTTTGTGATTTGAATTTATCATCCCGGTGAAGAACTCGTATTATGACATGTGAACTTTGAAACTTGGAATTAAATATTTAAATTATCATCCAGGCAACACCATTATTTGCTGGGATTACTGTTGCTGCTGCTGCTCTTGCTGGTAGATATGGAATACAAGCTTGGCAAGCATTCAAGGCACGTCCGCCTAGACCTAAAAAGTTTTTTGTAGGTGGCTTCCAACCCAAGATGACCAAGAGGGAAGCGGCTCTTATCCTTGGCGTAAGGTAGTTATCAGTTGTATGATAACTTCTCAATTCTTGTGCATTTGAAGGTTTGCTGGTATTAGCCAAGAAGTTTCTCTATATCATAAATTCATGACCAAGCAAACATCCTAATGTCTGTGCACGGCTAATTACCTGGAGAACTAGTATAAGTTAAAAATTAACTACTAAAGAGTAAAGACAAAAAGGTACCTGTAAGATAGGTTATTACGGAGTATAAAAGTTGTCATAAAAGGTGAACTAACCTAAAATGAAGGGCAGAACCAGAGTAATAAATTGATTTGAAGCTATCCAGTAGAGCTCAATTCAAGCTTTTTTATCTCTTGAATCTAGCAATGTCATTGTTTCACTTCATTTTACTGAATTCTCACGACATAGTGAATTATATTTCGTTAGTTCTCATCATGTTGATCTGGAAGCAATTTTTATCAGTGTAGAAAATACTACTACTTTCATGTGATTATTTTTTCTGTCCTTGTTAATGTTAAGCCTTTTTTTGGTCAAATTCTTCTTTTACTTTAGGCAGGAAACGGTTTCTTATATTTGAACTTGAGCTTACCTACCGGACTTGTGTTCGCAGTCTCACAGAGACTATGGGTAGCTCAAATTTCCAATGTCCCTTCTTAAGTTCTACGTGACTCAAGTTATTTAACTTCTGGCCTAGAAATTTGTTTATCGTAGCATTTGTTGGTATTTTCTAGGTAACTGGACATATGTCCCATATTGCGGTAAGTACCTTGAATACTGCCATTTCTTTTACTTTATAGCTTTTGCCACTTAGCCGGGTTCAAAGAAGGTTAATGATCGCTTAGTTCAATTCCAAAGTGTTTCTTTTACTCTGTAATTTATTCACTTATTTGACCTTCTCTTCCCCCTTTCAATATCTTGTTATCTGTGTGTGTTCGATATCTATTATCTATAGTCTCTCATACATTCGAAGTATATGTTACCAAATATTTCAAATTTTGAAATGCAATGAATAATTGAAATGACCCTCAGCTTATTTCTTACTCGGTATTCATTTTATAAGGCTTTTTGTTCTGTTGTAGTTTGGCTGTTGTCATGTTTATCTTTTTCATTCTTTCTCTCCAAGTGTTAGTATTCAACATATTTGTATGAACCTTAAAATGGCAGGGAGAGTGCGGTTGCAGAGAAGGTGAAGGAAGCTCATAGAAGGGTAATGATTGCAAACCATCCGGATGCAGGTGGAAGCCATTATCTTGCCTCTAAAATCAATGAAGCTAAGGACATGATGGTCGGGAAGCCCAAAGACAGCGGCTCTGCCTTCTAATGCCATTTTATGTTGACCCTCGGATATAAGATTATTGTCTGTTCCTCTTCACCGAAACCATGGCTAAATGCCTACCCCTGTATGTTTAAAGTGAACCAAGGAACTCAGATGCCCTTCTCTTTAGATAAGGAAAGGTGATTGAGCATACACAGGTTGCAGTTGGCTGCAATTTTAGAACGTGTACACTATTTGACTGGTATAAGACAACAGTCACCAAAGCTATTGGATTTAAATGACACTGTTTTCTTGTCATGTTACTGTTATTTTAAGCTATTTGGAATATAAGCCTTAGGGAATTTTAACTTGTACTCCCGAGGTTTCCACGAACGTAGCATGTAAATAAAGCCAACGTTTGAGAAGTTTGAATACTCTTCGTACTTACTTTATGCTGTTTTAAATTATCAATAAAATTATATACTTGGTATTCC

mRNA sequence

CACATGACCCAGAAAGAGATTTCTGAAGGGTTTTCGATAATTGGCCATCAAAGTGTTTTCATTGAACTTTCTAGCACATACTCGAAACTTCACGAGTAATCACGACGGAAACAAAGAACATCATTTCAAGCCAACTCTGAAGACAAATCCAAAGCTGAATTTCTTTGTCATAAAATCGTCATAAAAATGGCAACACCATTATTTGCTGGGATTACTGTTGCTGCTGCTGCTCTTGCTGGTAGATATGGAATACAAGCTTGGCAAGCATTCAAGGCACGTCCGCCTAGACCTAAAAAGTTTTTTGTAGGTGGCTTCCAACCCAAGATGACCAAGAGGGAAGCGGCTCTTATCCTTGGCGTAAGGGAGAGTGCGGTTGCAGAGAAGGTGAAGGAAGCTCATAGAAGGGTAATGATTGCAAACCATCCGGATGCAGGTGGAAGCCATTATCTTGCCTCTAAAATCAATGAAGCTAAGGACATGATGGTCGGGAAGCCCAAAGACAGCGGCTCTGCCTTCTAATGCCATTTTATGTTGACCCTCGGATATAAGATTATTGTCTGTTCCTCTTCACCGAAACCATGGCTAAATGCCTACCCCTGTATGTTTAAAGTGAACCAAGGAACTCAGATGCCCTTCTCTTTAGATAAGGAAAGGTGATTGAGCATACACAGGTTGCAGTTGGCTGCAATTTTAGAACGTGTACACTATTTGACTGGTATAAGACAACAGTCACCAAAGCTATTGGATTTAAATGACACTGTTTTCTTGTCATGTTACTGTTATTTTAAGCTATTTGGAATATAAGCCTTAGGGAATTTTAACTTGTACTCCCGAGGTTTCCACGAACGTAGCATGTAAATAAAGCCAACGTTTGAGAAGTTTGAATACTCTTCGTACTTACTTTATGCTGTTTTAAATTATCAATAAAATTATATACTTGGTATTCC

Coding sequence (CDS)

ATGGCAACACCATTATTTGCTGGGATTACTGTTGCTGCTGCTGCTCTTGCTGGTAGATATGGAATACAAGCTTGGCAAGCATTCAAGGCACGTCCGCCTAGACCTAAAAAGTTTTTTGTAGGTGGCTTCCAACCCAAGATGACCAAGAGGGAAGCGGCTCTTATCCTTGGCGTAAGGGAGAGTGCGGTTGCAGAGAAGGTGAAGGAAGCTCATAGAAGGGTAATGATTGCAAACCATCCGGATGCAGGTGGAAGCCATTATCTTGCCTCTAAAATCAATGAAGCTAAGGACATGATGGTCGGGAAGCCCAAAGACAGCGGCTCTGCCTTCTAA

Protein sequence

MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo22735	Spo22735	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo22735.1	Spo22735.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22735.1.utr5p.1	Spo22735.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22735.1.CDS.3	Spo22735.1.CDS.3	CDS
Spo22735.1.CDS.2	Spo22735.1.CDS.2	CDS
Spo22735.1.CDS.1	Spo22735.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22735.1.utr3p.1	Spo22735.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22735.1.exon.3	Spo22735.1.exon.3	exon
Spo22735.1.exon.2	Spo22735.1.exon.2	exon
Spo22735.1.exon.1	Spo22735.1.exon.1	exon

Homology

[Close]

BLAST of Spo22735.1 vs. NCBI nr
Match: gi|902229373|gb|KNA21482.1| (hypothetical protein SOVF_042630, partial [Spinacia oleracea])

HSP 1 Score: 216.9 bits (551), Expect = 1.800e-53
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE 60
           MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE
Sbjct: 58  MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE 117

Query: 61  SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF
Sbjct: 118 SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 167

[Close]

BLAST of Spo22735.1 vs. NCBI nr
Match: gi|731318349|ref|XP_010669671.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM14-1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 189.9 bits (481), Expect = 2.400e-45
Identity = 92/110 (83.64%), Postives = 103/110 (93.64%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE 60
           MATPL AG+ VAAAALAGRYGIQAWQ+FKARPP+P++F+ GGFQP MT+REAALILGVRE
Sbjct: 1   MATPLIAGLAVAAAALAGRYGIQAWQSFKARPPKPRRFYEGGFQPTMTRREAALILGVRE 60

Query: 61  SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           SA  EK+KEAHR+VMIANHPDAGGSHYLASKINEAKD+M+ KPKDSGSAF
Sbjct: 61  SAATEKIKEAHRKVMIANHPDAGGSHYLASKINEAKDVMLKKPKDSGSAF 110

[Close]

BLAST of Spo22735.1 vs. NCBI nr
Match: gi|255577663|ref|XP_002529708.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM14-1 [Ricinus communis])

HSP 1 Score: 186.4 bits (472), Expect = 2.600e-44
Identity = 92/112 (82.14%), Postives = 103/112 (91.96%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP FAG+ VAAAALAGRYGIQAWQAFKARPP+PK  KF+ GGFQPKMT+REAALILG+
Sbjct: 1   MATPFFAGLAVAAAALAGRYGIQAWQAFKARPPKPKFRKFYDGGFQPKMTRREAALILGI 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RE+A A+KVKEAHR+VM+ANHPDAGGSHYLASKINEAKD M+GK +D GSAF
Sbjct: 61  RENATADKVKEAHRKVMVANHPDAGGSHYLASKINEAKDTMLGKTRDGGSAF 112

[Close]

BLAST of Spo22735.1 vs. NCBI nr
Match: gi|802639800|ref|XP_012078541.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM14-1 [Jatropha curcas])

HSP 1 Score: 184.9 bits (468), Expect = 7.700e-44
Identity = 91/112 (81.25%), Postives = 103/112 (91.96%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP  AG+ VAAAALAGRYGIQAWQAFKARPP+PK  KF+ GGFQPKMT+REAALILG+
Sbjct: 1   MATPFLAGLAVAAAALAGRYGIQAWQAFKARPPKPKLRKFYDGGFQPKMTRREAALILGI 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RE+A A+KVKEAHR+VM+ANHPDAGGSHYLASKINEAKD+M+GK +D GSAF
Sbjct: 61  RENATADKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVMLGKTRDGGSAF 112

[Close]

BLAST of Spo22735.1 vs. NCBI nr
Match: gi|18403957|ref|NP_565824.1| (mitochondrial import inner membrane translocase subunit TIM14-1 [Arabidopsis thaliana])

HSP 1 Score: 184.5 bits (467), Expect = 1.000e-43
Identity = 93/112 (83.04%), Postives = 101/112 (90.18%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP  AG+ VAA ALAGRYGIQAWQAFKARPPRPK  KF+ GGFQP MTKREAALILGV
Sbjct: 1   MATPFIAGVAVAATALAGRYGIQAWQAFKARPPRPKIKKFYEGGFQPTMTKREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES  AEKVKEAHR+VM+ANHPDAGGSH+LASKINEAKD+M+GK K+SGSAF
Sbjct: 61  RESVAAEKVKEAHRKVMVANHPDAGGSHFLASKINEAKDVMLGKTKNSGSAF 112

[Close]

BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RRM9_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_042630 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.300e-53
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE 60
           MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE
Sbjct: 58  MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE 117

Query: 61  SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF
Sbjct: 118 SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 167

[Close]

BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CVC2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g036500 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.700e-45
Identity = 92/110 (83.64%), Postives = 103/110 (93.64%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPKKFFVGGFQPKMTKREAALILGVRE 60
           MATPL AG+ VAAAALAGRYGIQAWQ+FKARPP+P++F+ GGFQP MT+REAALILGVRE
Sbjct: 1   MATPLIAGLAVAAAALAGRYGIQAWQSFKARPPKPRRFYEGGFQPTMTRREAALILGVRE 60

Query: 61  SAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           SA  EK+KEAHR+VMIANHPDAGGSHYLASKINEAKD+M+ KPKDSGSAF
Sbjct: 61  SAATEKIKEAHRKVMIANHPDAGGSHYLASKINEAKDVMLKKPKDSGSAF 110

[Close]

BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
Match: B9SUN9_RICCO (Mitochondrial import inner membrane translocase subunit TIM14, putative OS=Ricinus communis GN=RCOM_0713680 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.800e-44
Identity = 92/112 (82.14%), Postives = 103/112 (91.96%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP FAG+ VAAAALAGRYGIQAWQAFKARPP+PK  KF+ GGFQPKMT+REAALILG+
Sbjct: 1   MATPFFAGLAVAAAALAGRYGIQAWQAFKARPPKPKFRKFYDGGFQPKMTRREAALILGI 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RE+A A+KVKEAHR+VM+ANHPDAGGSHYLASKINEAKD M+GK +D GSAF
Sbjct: 61  RENATADKVKEAHRKVMVANHPDAGGSHYLASKINEAKDTMLGKTRDGGSAF 112

[Close]

BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
Match: A0A0D2VXH1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G094000 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 1.600e-43
Identity = 92/112 (82.14%), Postives = 102/112 (91.07%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP FAG+ VAAAALAGRYGIQAWQAFKARPP+P+  KF+ GGFQP MT+REAALILGV
Sbjct: 1   MATPFFAGLAVAAAALAGRYGIQAWQAFKARPPKPRMRKFYDGGFQPTMTRREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RE+A AEKVKE HR+VM+ANHPDAGGSHYLASKINEAKD+M+GK K SGSAF
Sbjct: 61  RENATAEKVKEQHRKVMVANHPDAGGSHYLASKINEAKDVMLGKTKGSGSAF 112

[Close]

BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
Match: B9HLX8_POPTR (DNAJ heat shock N-terminal domain-containing family protein OS=Populus trichocarpa GN=POPTR_0008s04310g PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 2.000e-43
Identity = 89/112 (79.46%), Postives = 103/112 (91.96%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP  AG+ +AAAALAG+YG+QAWQ+FKARPP+P+  KF+ GGFQPKMT+REAALILG+
Sbjct: 1   MATPFLAGLAIAAAALAGKYGVQAWQSFKARPPKPRIRKFYDGGFQPKMTRREAALILGI 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RE+A AEKVKEAHRRVM+ANHPDAGGSHYLASKINEAKD+M+GK KD GSAF
Sbjct: 61  RENAGAEKVKEAHRRVMVANHPDAGGSHYLASKINEAKDVMLGKTKDGGSAF 112

[Close]

BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
Match: TI141_ARATH (Mitochondrial import inner membrane translocase subunit TIM14-1 OS=Arabidopsis thaliana GN=TIM14-1 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 6.300e-46
Identity = 93/112 (83.04%), Postives = 101/112 (90.18%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP  AG+ VAA ALAGRYGIQAWQAFKARPPRPK  KF+ GGFQP MTKREAALILGV
Sbjct: 1   MATPFIAGVAVAATALAGRYGIQAWQAFKARPPRPKIKKFYEGGFQPTMTKREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES  AEKVKEAHR+VM+ANHPDAGGSH+LASKINEAKD+M+GK K+SGSAF
Sbjct: 61  RESVAAEKVKEAHRKVMVANHPDAGGSHFLASKINEAKDVMLGKTKNSGSAF 112

[Close]

BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
Match: TI143_ARATH (Mitochondrial import inner membrane translocase subunit TIM14-3 OS=Arabidopsis thaliana GN=TIM14-3 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 6.500e-43
Identity = 89/112 (79.46%), Postives = 99/112 (88.39%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARP--PRPKKFFVGGFQPKMTKREAALILGV 60
           MATP+ AG  VAAAA+AGRYGI AWQAFKARP  PR ++F+ GGFQ  MT+REAALILGV
Sbjct: 1   MATPMIAGAAVAAAAVAGRYGILAWQAFKARPRVPRMRRFYEGGFQSSMTRREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES VA+KVKEAHRRVM+ANHPDAGGSHYLASKINEAKDMM+GK  +SGSAF
Sbjct: 61  RESVVADKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKSNNSGSAF 112

[Close]

BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
Match: TI142_ARATH (Mitochondrial import inner membrane translocase subunit TIM14-2 OS=Arabidopsis thaliana GN=TIM14-2 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 2.300e-40
Identity = 85/112 (75.89%), Postives = 94/112 (83.93%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           M   + AG  VAAAA AG+YGI+AWQAFK RP RP+  KF+ GGFQ  M +REAALILGV
Sbjct: 1   MVAAIIAGAAVAAAAYAGKYGIEAWQAFKLRPVRPRMRKFYEGGFQATMNRREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES  AEKVKEAHRRVM+ANHPDAGGSHYLASKINEAKDMM+GK K+SGSAF
Sbjct: 61  RESVAAEKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKTKNSGSAF 112

[Close]

BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
Match: TIM14_BOVIN (Mitochondrial import inner membrane translocase subunit TIM14 OS=Bos taurus GN=DNAJC19 PE=3 SV=3)

HSP 1 Score: 107.1 bits (266), Expect = 1.300e-22
Identity = 57/113 (50.44%), Postives = 78/113 (69.03%), Query Frame = 1


		  
Query: 2   ATPLFAGITVAAAALAGRYGIQAW--------QAFKARPPRPKK--FFVGGFQPKMTKRE 61
           +T +  G+T+AAA  AGRY +QA         Q F++ P       ++ GGF+PKMTKRE
Sbjct: 3   STVVAVGLTIAAAGFAGRYALQAMKHMEPQVKQVFQSLPKTAFSGGYYRGGFEPKMTKRE 62

Query: 62  AALILGVRESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPK 105
           AALILGV  +A   K+++AHRR+M+ NHPD GGS Y+A+KINEAKD++ G+ K
Sbjct: 63  AALILGVSPTANKAKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAK 115

[Close]

BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
Match: TIM14_HUMAN (Mitochondrial import inner membrane translocase subunit TIM14 OS=Homo sapiens GN=DNAJC19 PE=1 SV=3)

HSP 1 Score: 105.5 bits (262), Expect = 3.700e-22
Identity = 57/113 (50.44%), Postives = 78/113 (69.03%), Query Frame = 1


		  
Query: 2   ATPLFAGITVAAAALAGRYGIQAW--------QAFKARPPRPKK--FFVGGFQPKMTKRE 61
           +T +  G+T+AAA  AGRY +QA         Q F++ P       ++ GGF+PKMTKRE
Sbjct: 3   STVVAVGLTIAAAGFAGRYVLQAMKHMEPQVKQVFQSLPKSAFSGGYYRGGFEPKMTKRE 62

Query: 62  AALILGVRESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPK 105
           AALILGV  +A   K+++AHRR+M+ NHPD GGS Y+A+KINEAKD++ G+ K
Sbjct: 63  AALILGVSPTANKGKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAK 115

[Close]

BLAST of Spo22735.1 vs. TAIR (Arabidopsis)
Match: AT2G35795.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 184.5 bits (467), Expect = 3.500e-47
Identity = 93/112 (83.04%), Postives = 101/112 (90.18%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           MATP  AG+ VAA ALAGRYGIQAWQAFKARPPRPK  KF+ GGFQP MTKREAALILGV
Sbjct: 1   MATPFIAGVAVAATALAGRYGIQAWQAFKARPPRPKIKKFYEGGFQPTMTKREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES  AEKVKEAHR+VM+ANHPDAGGSH+LASKINEAKD+M+GK K+SGSAF
Sbjct: 61  RESVAAEKVKEAHRKVMVANHPDAGGSHFLASKINEAKDVMLGKTKNSGSAF 112

[Close]

BLAST of Spo22735.1 vs. TAIR (Arabidopsis)
Match: AT5G03030.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 174.5 bits (441), Expect = 3.700e-44
Identity = 89/112 (79.46%), Postives = 99/112 (88.39%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARP--PRPKKFFVGGFQPKMTKREAALILGV 60
           MATP+ AG  VAAAA+AGRYGI AWQAFKARP  PR ++F+ GGFQ  MT+REAALILGV
Sbjct: 1   MATPMIAGAAVAAAAVAGRYGILAWQAFKARPRVPRMRRFYEGGFQSSMTRREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES VA+KVKEAHRRVM+ANHPDAGGSHYLASKINEAKDMM+GK  +SGSAF
Sbjct: 61  RESVVADKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKSNNSGSAF 112

[Close]

BLAST of Spo22735.1 vs. TAIR (Arabidopsis)
Match: AT3G09700.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 166.0 bits (419), Expect = 1.300e-41
Identity = 85/112 (75.89%), Postives = 94/112 (83.93%), Query Frame = 1


		  
Query: 1   MATPLFAGITVAAAALAGRYGIQAWQAFKARPPRPK--KFFVGGFQPKMTKREAALILGV 60
           M   + AG  VAAAA AG+YGI+AWQAFK RP RP+  KF+ GGFQ  M +REAALILGV
Sbjct: 1   MVAAIIAGAAVAAAAYAGKYGIEAWQAFKLRPVRPRMRKFYEGGFQATMNRREAALILGV 60

Query: 61  RESAVAEKVKEAHRRVMIANHPDAGGSHYLASKINEAKDMMVGKPKDSGSAF 111
           RES  AEKVKEAHRRVM+ANHPDAGGSHYLASKINEAKDMM+GK K+SGSAF
Sbjct: 61  RESVAAEKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKTKNSGSAF 112

The following BLAST results are available for this feature:

BLAST of Spo22735.1 vs. NCBI nr
BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
BLAST of Spo22735.1 vs. TAIR (Arabidopsis)

BLAST of Spo22735.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902229373\|gb\|KNA21482.1\|	1.8e-53	100.	hypothetical protein SOVF_0426...	[more]
gi\|731318349\|ref\|XP_010669671.1\|	2.4e-45	83.6	PREDICTED: mitochondrial impor...	[more]
gi\|255577663\|ref\|XP_002529708.1\|	2.6e-44	82.1	PREDICTED: mitochondrial impor...	[more]
gi\|802639800\|ref\|XP_012078541.1\|	7.7e-44	81.2	PREDICTED: mitochondrial impor...	[more]
gi\|18403957\|ref\|NP_565824.1\|	1.0e-43	83.0	mitochondrial import inner mem...	[more]

BLAST of Spo22735.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RRM9_SPIOL	1.3e-53	100.	Uncharacterized protein (Fragm...	[more]
A0A0J8CVC2_BETVU	1.7e-45	83.6	Uncharacterized protein OS=Bet...	[more]
B9SUN9_RICCO	1.8e-44	82.1	Mitochondrial import inner mem...	[more]
A0A0D2VXH1_GOSRA	1.6e-43	82.1	Uncharacterized protein OS=Gos...	[more]
B9HLX8_POPTR	2.0e-43	79.4	DNAJ heat shock N-terminal dom...	[more]

BLAST of Spo22735.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
TI141_ARATH	6.3e-46	83.0	Mitochondrial import inner mem...	[more]
TI143_ARATH	6.5e-43	79.4	Mitochondrial import inner mem...	[more]
TI142_ARATH	2.3e-40	75.8	Mitochondrial import inner mem...	[more]
TIM14_BOVIN	1.3e-22	50.4	Mitochondrial import inner mem...	[more]
TIM14_HUMAN	3.7e-22	50.4	Mitochondrial import inner mem...	[more]

BLAST of Spo22735.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT2G35795.1	3.5e-47	83.0	Chaperone DnaJ-domain superfam...	[more]
AT5G03030.1	3.7e-44	79.4	Chaperone DnaJ-domain superfam...	[more]
AT3G09700.1	1.3e-41	75.8	Chaperone DnaJ-domain superfam...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	GENE3D	1.10.287.110		coord: 41..104 score: 9.5
IPR001623	DnaJ domain	unknown	SSF46565	Chaperone J-domain	coord: 41..102 score: 3.27
None	No IPR available	PANTHER	PTHR12763	UNCHARACTERIZED	coord: 1..110 score: 4.2
None	No IPR available	PANTHER	PTHR12763:SF30	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM14-1	coord: 1..110 score: 4.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005743	mitochondrial inner membrane