Spo22945.1 (mRNA) | SpinachBase

Overview


Name	Spo22945.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	AT3g10020/T22K18_16
Location	SpoScf_02555 : 36692 .. 37090 (-)
Sequence length	399

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACACAATAAGAGGGGACGAGTCAGTGGTAATCCGAGGAAAACACCGGGACGAGGAAGGGAGAGTAGTGGTGGAGAAAGTAGAGGTGCACACCCACGACAAAGACACCCTGAAGCATCTCGAGAAGAAGCTCGTAGACACGGGACTCCACCGCCTTGAGCGTCACTCTGCTGATAACAGGGCATCGGTGAAGAAGCCACCTCCAAAGAAAGGACACGGGGGTAAGTATACATGGGAAGGGCCTGACCTTGAGGTTGAGAATGAGTTTGAAGCTGAACTTGTTCTCGACAAAGGGGATCCTAATTATGTTGATCAAGAAGAGGAGCTGGATCATGAGGTCAAAGAGAGGATCAAGGGTCAACTGGAAGTTCCTAAGCTGCCTCAGGCTAGAGAGTAG

mRNA sequence

ATGAACACAATAAGAGGGGACGAGTCAGTGGTAATCCGAGGAAAACACCGGGACGAGGAAGGGAGAGTAGTGGTGGAGAAAGTAGAGGTGCACACCCACGACAAAGACACCCTGAAGCATCTCGAGAAGAAGCTCGTAGACACGGGACTCCACCGCCTTGAGCGTCACTCTGCTGATAACAGGGCATCGGTGAAGAAGCCACCTCCAAAGAAAGGACACGGGGGTAAGTATACATGGGAAGGGCCTGACCTTGAGGTTGAGAATGAGTTTGAAGCTGAACTTGTTCTCGACAAAGGGGATCCTAATTATGTTGATCAAGAAGAGGAGCTGGATCATGAGGTCAAAGAGAGGATCAAGGGTCAACTGGAAGTTCCTAAGCTGCCTCAGGCTAGAGAGTAG

Coding sequence (CDS)

ATGAACACAATAAGAGGGGACGAGTCAGTGGTAATCCGAGGAAAACACCGGGACGAGGAAGGGAGAGTAGTGGTGGAGAAAGTAGAGGTGCACACCCACGACAAAGACACCCTGAAGCATCTCGAGAAGAAGCTCGTAGACACGGGACTCCACCGCCTTGAGCGTCACTCTGCTGATAACAGGGCATCGGTGAAGAAGCCACCTCCAAAGAAAGGACACGGGGGTAAGTATACATGGGAAGGGCCTGACCTTGAGGTTGAGAATGAGTTTGAAGCTGAACTTGTTCTCGACAAAGGGGATCCTAATTATGTTGATCAAGAAGAGGAGCTGGATCATGAGGTCAAAGAGAGGATCAAGGGTCAACTGGAAGTTCCTAAGCTGCCTCAGGCTAGAGAGTAG

Protein sequence

MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKPPPKKGHGGKYTWEGPDLEVENEFEAELVLDKGDPNYVDQEEELDHEVKERIKGQLEVPKLPQARE

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo22945	Spo22945	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo22945.1	Spo22945.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22945.1.CDS.1	Spo22945.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22945.1.exon.1	Spo22945.1.exon.1	exon

Homology

[Close]

BLAST of Spo22945.1 vs. NCBI nr
Match: gi|902222380|gb|KNA19160.1| (hypothetical protein SOVF_064140 [Spinacia oleracea])

HSP 1 Score: 260.4 bits (664), Expect = 1.700e-66
Identity = 130/132 (98.48%), Postives = 131/132 (99.24%), Query Frame = 1


		  
Query: 1   MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADN 60
           MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADN
Sbjct: 1   MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADN 60

Query: 61  RASVKKPPPKKGHGGKYTWEGPDLEVENEFEAELVLDKGDPNYVDQEEELDHEVKERIKG 120
           RASVKKPPPKKGHGGKYTWEGPDLEVENEFEA+LVLDKGDPNYVDQEEELDHEVKERIKG
Sbjct: 61  RASVKKPPPKKGHGGKYTWEGPDLEVENEFEADLVLDKGDPNYVDQEEELDHEVKERIKG 120

Query: 121 QLEVPKLPQARE 133
           QLEV KLPQARE
Sbjct: 121 QLEVAKLPQARE 132

[Close]

BLAST of Spo22945.1 vs. NCBI nr
Match: gi|731345383|ref|XP_010683892.1| (PREDICTED: uncharacterized protein LOC104898501 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 173.7 bits (439), Expect = 2.100e-40
Identity = 87/129 (67.44%), Postives = 108/129 (83.72%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           E+V +R KHRDEEG++V EKVEVHTHD D+LKH+EKKLVDTGLHRLERHSAD++ASVKKP
Sbjct: 10  ETVTLRAKHRDEEGKIVTEKVEVHTHDVDSLKHVEKKLVDTGLHRLERHSADHKASVKKP 69

Query: 68  PPKKGHGGKYTWEGPDLEVENEFEAEL-VLDKGDPNYVDQEEEL---DHEVKERIKGQLE 127
           PPKKG GGKYTWEGP  E + E EA + VLD+GDPN+VD E+E+   D EV++ +KG++E
Sbjct: 70  PPKKGRGGKYTWEGPMTEADVELEAGVPVLDEGDPNFVDDEKEVGGDDEEVRDMVKGEVE 129

Query: 128 VPKLPQARE 133
           V K+ + RE
Sbjct: 130 VAKVVEGRE 138

[Close]

BLAST of Spo22945.1 vs. NCBI nr
Match: gi|802562702|ref|XP_012066508.1| (PREDICTED: uncharacterized protein LOC105629509 [Jatropha curcas])

HSP 1 Score: 128.3 bits (321), Expect = 1.000e-26
Identity = 66/123 (53.66%), Postives = 88/123 (71.54%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           + VVIR    DEEGR  VEK E+HTH+KDT+K++EKKL+D G+HR++RH  D    ++KP
Sbjct: 9   DKVVIRAVSHDEEGRKRVEKTELHTHNKDTIKYVEKKLMDKGVHRMDRHPVDGIGGIRKP 68

Query: 68  PPKKGHGGKYTWEGPDLEVENEFE-AELVLDKGDPNYVDQEEE---LDHEVKERIKGQLE 127
           PPK GHGGKYTWEGP+   E+E + A   +D+ DPNYVD+E+E   L  E  E + G++E
Sbjct: 69  PPKSGHGGKYTWEGPEDMAESELDAAPPAIDEKDPNYVDEEKEEKLLRGEEGELVVGEVE 128

[Close]

BLAST of Spo22945.1 vs. NCBI nr
Match: gi|1000947750|ref|XP_015580496.1| (PREDICTED: uncharacterized protein LOC107262006 [Ricinus communis])

HSP 1 Score: 123.6 bits (309), Expect = 2.500e-25
Identity = 65/126 (51.59%), Postives = 87/126 (69.05%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           E VVIR    DEEGR  VEK E++TH+ DT+K++EKKL+D G+HR++RH AD +  + KP
Sbjct: 9   EKVVIRAVSHDEEGRKRVEKTELNTHNIDTIKYVEKKLMDKGVHRMDRHPADGKGGIGKP 68

Query: 68  PPKKGHGGKYTWEGPDLEVENEFE-AELVLDKGDPNYVDQEEE------LDHEVKERIKG 127
           PPK GHGGKYTWEGPD   E+E + A   +D+ DPNYVD+E E       + +V   + G
Sbjct: 69  PPKSGHGGKYTWEGPDGTTESELDAAPAAIDEKDPNYVDEEAEEKILRGENSDVAGFVVG 128

[Close]

BLAST of Spo22945.1 vs. NCBI nr
Match: gi|703160426|ref|XP_010112524.1| (hypothetical protein L484_012802 [Morus notabilis])

HSP 1 Score: 122.1 bits (305), Expect = 7.300e-25
Identity = 67/132 (50.76%), Postives = 86/132 (65.15%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           ++VVIR   RDEEGR  VEK  + THD DT+K++EKKL+D G+ RL+RH AD    + KP
Sbjct: 9   DTVVIRAVSRDEEGRKRVEKTGLKTHDVDTIKYVEKKLIDKGVQRLDRHPADGIGGIGKP 68

Query: 68  PPKKGHGGKYTWEGPDLEVENEF-EAELVLDKGDPNYVDQEEE------LDHEVKERIKG 127
           PPK GHGGKYTWEGP    EN    A   +D+ DPNYVD++ E       D +V E + G
Sbjct: 69  PPKSGHGGKYTWEGPGEVAENLLAPAPAAIDERDPNYVDEDAEERIVRGEDKDVGELVVG 128

Query: 128 QLEVPKLPQARE 133
           ++EV K  + RE
Sbjct: 129 EVEVAKAAEGRE 140

[Close]

BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RI60_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_064140 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.200e-66
Identity = 130/132 (98.48%), Postives = 131/132 (99.24%), Query Frame = 1


		  
Query: 1   MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADN 60
           MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADN
Sbjct: 1   MNTIRGDESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADN 60

Query: 61  RASVKKPPPKKGHGGKYTWEGPDLEVENEFEAELVLDKGDPNYVDQEEELDHEVKERIKG 120
           RASVKKPPPKKGHGGKYTWEGPDLEVENEFEA+LVLDKGDPNYVDQEEELDHEVKERIKG
Sbjct: 61  RASVKKPPPKKGHGGKYTWEGPDLEVENEFEADLVLDKGDPNYVDQEEELDHEVKERIKG 120

Query: 121 QLEVPKLPQARE 133
           QLEV KLPQARE
Sbjct: 121 QLEVAKLPQARE 132

[Close]

BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BXU2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g160420 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 1.500e-40
Identity = 87/129 (67.44%), Postives = 108/129 (83.72%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           E+V +R KHRDEEG++V EKVEVHTHD D+LKH+EKKLVDTGLHRLERHSAD++ASVKKP
Sbjct: 10  ETVTLRAKHRDEEGKIVTEKVEVHTHDVDSLKHVEKKLVDTGLHRLERHSADHKASVKKP 69

Query: 68  PPKKGHGGKYTWEGPDLEVENEFEAEL-VLDKGDPNYVDQEEEL---DHEVKERIKGQLE 127
           PPKKG GGKYTWEGP  E + E EA + VLD+GDPN+VD E+E+   D EV++ +KG++E
Sbjct: 70  PPKKGRGGKYTWEGPMTEADVELEAGVPVLDEGDPNFVDDEKEVGGDDEEVRDMVKGEVE 129

Query: 128 VPKLPQARE 133
           V K+ + RE
Sbjct: 130 VAKVVEGRE 138

[Close]

BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
Match: A0A067L2Z2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23702 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.100e-27
Identity = 66/123 (53.66%), Postives = 88/123 (71.54%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           + VVIR    DEEGR  VEK E+HTH+KDT+K++EKKL+D G+HR++RH  D    ++KP
Sbjct: 9   DKVVIRAVSHDEEGRKRVEKTELHTHNKDTIKYVEKKLMDKGVHRMDRHPVDGIGGIRKP 68

Query: 68  PPKKGHGGKYTWEGPDLEVENEFE-AELVLDKGDPNYVDQEEE---LDHEVKERIKGQLE 127
           PPK GHGGKYTWEGP+   E+E + A   +D+ DPNYVD+E+E   L  E  E + G++E
Sbjct: 69  PPKSGHGGKYTWEGPEDMAESELDAAPPAIDEKDPNYVDEEKEEKLLRGEEGELVVGEVE 128

[Close]

BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
Match: W9SB48_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_012802 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.100e-25
Identity = 67/132 (50.76%), Postives = 86/132 (65.15%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           ++VVIR   RDEEGR  VEK  + THD DT+K++EKKL+D G+ RL+RH AD    + KP
Sbjct: 9   DTVVIRAVSRDEEGRKRVEKTGLKTHDVDTIKYVEKKLIDKGVQRLDRHPADGIGGIGKP 68

Query: 68  PPKKGHGGKYTWEGPDLEVENEF-EAELVLDKGDPNYVDQEEE------LDHEVKERIKG 127
           PPK GHGGKYTWEGP    EN    A   +D+ DPNYVD++ E       D +V E + G
Sbjct: 69  PPKSGHGGKYTWEGPGEVAENLLAPAPAAIDERDPNYVDEDAEERIVRGEDKDVGELVVG 128

Query: 128 QLEVPKLPQARE 133
           ++EV K  + RE
Sbjct: 129 EVEVAKAAEGRE 140

[Close]

BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
Match: A0A0L9TF36_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan641s001300 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 7.400e-24
Identity = 66/126 (52.38%), Postives = 85/126 (67.46%), Query Frame = 1


		  
Query: 8   ESVVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHSADNRASVKKP 67
           E V IR   RDEEGR  VEK EV+TH+ DTLK++EKKL++ G+ R +RH AD    + +P
Sbjct: 9   EKVTIRAVSRDEEGRKRVEKREVNTHNADTLKYVEKKLINKGIQRQDRHPADG-IGIGRP 68

Query: 68  PPKKGHGGKYTWEGPDLEVENE-FEAELVLDKGDPNYVDQEEELDHEVKERIKGQLEVPK 127
           PPK GHGGK+TWEGP    ENE   A   +D+GDPNY D EE+   EV E + G++EV K
Sbjct: 69  PPKSGHGGKFTWEGPSDMAENELMAAPAAIDEGDPNYEDGEEK-GEEVAELVVGEVEVAK 128

Query: 128 LPQARE 133
           + Q  +
Sbjct: 129 VGQEHD 132

[Close]

BLAST of Spo22945.1 vs. TAIR (Arabidopsis)
Match: AT3G10020.1 (unknown protein)

HSP 1 Score: 100.9 bits (250), Expect = 6.200e-22
Identity = 55/132 (41.67%), Postives = 88/132 (66.67%), Query Frame = 1


		  
Query: 1   MNTIRGDES---VVIRGKHRDEEGRVVVEKVEVHTHDKDTLKHLEKKLVDTGLHRLERHS 60
           M  +  DE+   VV+R +H++EEGR  V+K EV T + +T+K +E+KL++ G+ R++RH 
Sbjct: 1   MQAVSVDETKNTVVLRAEHKNEEGRKSVDKFEVKTRNPETIKSVERKLMEKGVQRMDRHP 60

Query: 61  ADNRASVKKPPPKKGHGGKYTWEGPDLEVENEFEAE-LVLDKGDPNYVDQE--EELDHEV 120
           +D    +++PP K GHGGKYTWEGPD   + E + +   +D+GDPNY +++  +  D   
Sbjct: 61  SDG-IPLRRPPSKSGHGGKYTWEGPDRMEDYEMQPDPPAMDEGDPNYDEEQVKKSGDDVA 120

Query: 121 KERIKGQLEVPK 127
            E +KG++EV K
Sbjct: 121 VELVKGEVEVAK 131

The following BLAST results are available for this feature:

BLAST of Spo22945.1 vs. NCBI nr
BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
BLAST of Spo22945.1 vs. ExPASy Swiss-Prot
BLAST of Spo22945.1 vs. TAIR (Arabidopsis)

BLAST of Spo22945.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902222380\|gb\|KNA19160.1\|	1.7e-66	98.4	hypothetical protein SOVF_0641...	[more]
gi\|731345383\|ref\|XP_010683892.1\|	2.1e-40	67.4	PREDICTED: uncharacterized pro...	[more]
gi\|802562702\|ref\|XP_012066508.1\|	1.0e-26	53.6	PREDICTED: uncharacterized pro...	[more]
gi\|1000947750\|ref\|XP_015580496.1\|	2.5e-25	51.5	PREDICTED: uncharacterized pro...	[more]
gi\|703160426\|ref\|XP_010112524.1\|	7.3e-25	50.7	hypothetical protein L484_0128...	[more]

BLAST of Spo22945.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RI60_SPIOL	1.2e-66	98.4	Uncharacterized protein OS=Spi...	[more]
A0A0J8BXU2_BETVU	1.5e-40	67.4	Uncharacterized protein OS=Bet...	[more]
A0A067L2Z2_JATCU	7.1e-27	53.6	Uncharacterized protein OS=Jat...	[more]
W9SB48_9ROSA	5.1e-25	50.7	Uncharacterized protein OS=Mor...	[more]
A0A0L9TF36_PHAAN	7.4e-24	52.3	Uncharacterized protein OS=Pha...	[more]

BLAST of Spo22945.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo22945.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G10020.1	6.2e-22	41.6	unknown protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12626	PROGRAMMED CELL DEATH 4	coord: 7..130 score: 2.0
None	No IPR available	PANTHER	PTHR12626:SF7	SUBFAMILY NOT NAMED	coord: 7..130 score: 2.0