Spo24183.1 (mRNA)

Overview
NameSpo24183.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionDNA repair protein rev1
LocationSpoScf_00807 : 123389 .. 125033 (+)
Sequence length436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGTTGATTGGGTCTTTGAATTGGAGATAAAAGAAATTGGTGAATGGGTCTTTGAATTGGAGATGAGAGAAATTGGTGAATCGTGTTTCTGAATTGGCGAGAAGAACCAGGTTATTTGTATATTTCTCTTCGAATTTCCTCATAAATTAAGAACTTGGGTTGTTTAAATTATTATCGATTAAAATTGGATTTTTTTTGTGTGGTTTTATGGCTAATTGGGGTTTTTTAGTTAGGGAAATTCGTATGATTTTTCTCTCAGGTTTTTTTTGATGCGTGTACGATTTAATTACAGAAATATTTGTTGATTTCACAATATCAATCAATTTTGGGGATCGATCAATTGGTTGTTTTGGATGAATTTAGTTCTAAATTTTGGTAGAATTGTGTGAACGATTATATTAAACTGATTGATATTGATTATTATCAGTTTTGATAGTTGATGGTGCTTGAAATCAATGAAGTTATCCCGAATGTATTCATCTTCTGAATTTGTATAGTGTTTTCTCCTCCTATTTCGTTTTGTAATACCCTTTTCTTCAGTTGAATACTTGCCCATCATAAACTTCCTGTAATTAGTTTGTTGTAATTGTTTGTACAGGTCCCTGTGATGTTTTGTTTTCTGGTTGTTAGTTTCTGGTCCTTCCTAGAAGGCTGGAAACACAACTTGAACTTAGGTTGTAAAAATGGTGTTTGGATTTTGAATATAGTTGCCATAGCTTAATTTGAATATAGGCAGGAAGGGCATAAACTTAAGTTCTTTTGGACTGATCTTTGAAACTACAGGTTGCACAGCTAGTGCAGGGATTGCTGCAAATATGCTTATAGCTCGTCTTGCCATCAGAACTGCTAAACCAGATGGTCAATGCTAAATTTCTCCAGAGAAGGTATTCGTAAGAGTTTCATGTTAAATGTTTTTGGTAAAAGAAACTCAATTAAGTTTCATGACTGCATGAAATGAGCTTGTGCAAAGCTCAACTCGATCTTGCTTAGTCAAGATTGCTTGATTATGATTCATGCAAGATTTAAATTTCTGCATGCACAAAGTTTCTTGATTATGATTCATGCAAGATTTTTTGTGGCTGTCGTTATCAGGAACTGTCCAGAATTGATGGACAAGCCTGTAGCAGTGTGCCATTCAGACAGTCCTAGAGGAACTTCTGAAATTTCTTCTGCTAACTATCCTGCTCGAGATTATGGTTGTTTTGAAATCCTTGTGCCAGTAAGCATTGTGGTTTAAATACACACTTTTTTGTTATTCATATTCAAGTGGTTTTGTGTTTCTCGGAATAGGGGTTAAAGCTGGAATGTTCGTCAGAGATGCCAAGGCTCGTTGTCCTCATCTGGAAATCGTACCTTACAATTTTGAAGCTTATGAAGAGGTATTGTACCACCCCCCCCACCCCCCACCCCACCCCCTCTAAAACATAAGGGGAGAGGGGATAATATTGAAGTCCTGATTTTTGTGTCTTTAGAGCTATATGAATCATCTGTTGTTGTGACAGGTAGCTGACTAATTCTACACCATATTGCATAAACACTGCGATAAAGTGCATGCATGTGTTAAAATATCCTATACAATTTAATCTTCACTGTCAGTATGTTAGTGTTCTTTGACAGAATATCCGTTTACCTGCAGGCAGTAA

mRNA sequence

AATTGTTGATTGGGTCTTTGAATTGGAGATAAAAGAAATTGGTGAATGGGTCTTTGAATTGGAGATGAGAGAAATTGGTGAATCGTGTTTCTGAATTGGCGAGAAGAACCAGGTTGCACAGCTAGTGCAGGGATTGCTGCAAATATGCTTATAGCTCGTCTTGCCATCAGAACTGCTAAACCAGATGGTCAATGCTAAATTTCTCCAGAGAAGGAACTGTCCAGAATTGATGGACAAGCCTGTAGCAGTGTGCCATTCAGACAGTCCTAGAGGAACTTCTGAAATTTCTTCTGCTAACTATCCTGCTCGAGATTATGGTTGTTTTGAAATCCTTGTGCCAGGGTTAAAGCTGGAATGTTCGTCAGAGATGCCAAGGCTCGTTGTCCTCATCTGGAAATCGTACCTTACAATTTTGAAGCTTATGAAGAGGCAGTAA

Coding sequence (CDS)

ATGGTCAATGCTAAATTTCTCCAGAGAAGGAACTGTCCAGAATTGATGGACAAGCCTGTAGCAGTGTGCCATTCAGACAGTCCTAGAGGAACTTCTGAAATTTCTTCTGCTAACTATCCTGCTCGAGATTATGGTTGTTTTGAAATCCTTGTGCCAGGGTTAAAGCTGGAATGTTCGTCAGAGATGCCAAGGCTCGTTGTCCTCATCTGGAAATCGTACCTTACAATTTTGAAGCTTATGAAGAGGCAGTAA

Protein sequence

MVNAKFLQRRNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGCFEILVPGLKLECSSEMPRLVVLIWKSYLTILKLMKRQ
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo24183Spo24183gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo24183.1Spo24183.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo24183.1.exon.1Spo24183.1.exon.1exon
Spo24183.1.exon.2Spo24183.1.exon.2exon
Spo24183.1.exon.3Spo24183.1.exon.3exon
Spo24183.1.exon.4Spo24183.1.exon.4exon
Spo24183.1.exon.5Spo24183.1.exon.5exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo24183.1.utr5p.1Spo24183.1.utr5p.1five_prime_UTR
Spo24183.1.utr5p.2Spo24183.1.utr5p.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo24183.1.CDS.1Spo24183.1.CDS.1CDS
Spo24183.1.CDS.2Spo24183.1.CDS.2CDS
Spo24183.1.CDS.3Spo24183.1.CDS.3CDS
Spo24183.1.CDS.4Spo24183.1.CDS.4CDS


Homology
[Close]
BLAST of Spo24183.1 vs. NCBI nr
Match: gi|902164130|gb|KNA06932.1| (hypothetical protein SOVF_176540 [Spinacia oleracea])

HSP 1 Score: 90.5 bits (223), Expect = 1.500e-15
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGCFEILVP 53
           RN PELMDKPVAVCHSDSPRGTSEISSANYPARDYGCF+ILVP
Sbjct: 389 RNRPELMDKPVAVCHSDSPRGTSEISSANYPARDYGCFKILVP 431
[Close]
BLAST of Spo24183.1 vs. NCBI nr
Match: gi|731360148|ref|XP_010691659.1| (PREDICTED: DNA repair protein REV1 isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 74.7 bits (182), Expect = 8.400e-11
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGC-FEILVPGLKLECSSEMPRLVVL 69
           RN P+LMDKPVAVCHSDSPRGT+EISSANYPARDYG    + V   K  C    P LV++
Sbjct: 413 RNRPDLMDKPVAVCHSDSPRGTAEISSANYPARDYGVKAGMFVRDAKARC----PHLVIV 468
[Close]
BLAST of Spo24183.1 vs. NCBI nr
Match: gi|731360144|ref|XP_010691657.1| (PREDICTED: DNA repair protein REV1 isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 74.7 bits (182), Expect = 8.400e-11
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGC-FEILVPGLKLECSSEMPRLVVL 69
           RN P+LMDKPVAVCHSDSPRGT+EISSANYPARDYG    + V   K  C    P LV++
Sbjct: 413 RNRPDLMDKPVAVCHSDSPRGTAEISSANYPARDYGVKAGMFVRDAKARC----PHLVIV 468
[Close]
BLAST of Spo24183.1 vs. NCBI nr
Match: gi|731360150|ref|XP_010691660.1| (PREDICTED: DNA repair protein REV1 isoform X3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 74.7 bits (182), Expect = 8.400e-11
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGC-FEILVPGLKLECSSEMPRLVVL 69
           RN P+LMDKPVAVCHSDSPRGT+EISSANYPARDYG    + V   K  C    P LV++
Sbjct: 413 RNRPDLMDKPVAVCHSDSPRGTAEISSANYPARDYGVKAGMFVRDAKARC----PHLVIV 468
[Close]
BLAST of Spo24183.1 vs. NCBI nr
Match: gi|764524689|ref|XP_011457814.1| (PREDICTED: DNA repair protein REV1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 73.6 bits (179), Expect = 1.900e-10
Identity = 37/60 (61.67%), Postives = 43/60 (71.67%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGC-FEILVPGLKLECSSEMPRLVVL 69
           R CPEL D+PVAVCHSD+P+GT+EISSANYPARDYG    + V   K  C    P LV+L
Sbjct: 389 RQCPELKDRPVAVCHSDNPKGTAEISSANYPARDYGVRAGMFVRDAKARC----PHLVIL 444
[Close]
BLAST of Spo24183.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QI35_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_176540 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.000e-15
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGCFEILVP 53
           RN PELMDKPVAVCHSDSPRGTSEISSANYPARDYGCF+ILVP
Sbjct: 389 RNRPELMDKPVAVCHSDSPRGTSEISSANYPARDYGCFKILVP 431
[Close]
BLAST of Spo24183.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EDR7_BETVU (DNA repair protein REV1 OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g223680 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 5.900e-11
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGC-FEILVPGLKLECSSEMPRLVVL 69
           RN P+LMDKPVAVCHSDSPRGT+EISSANYPARDYG    + V   K  C    P LV++
Sbjct: 413 RNRPDLMDKPVAVCHSDSPRGTAEISSANYPARDYGVKAGMFVRDAKARC----PHLVIV 468
[Close]
BLAST of Spo24183.1 vs. UniProtKB/TrEMBL
Match: A0A022RUT3_ERYGU (DNA repair protein REV1 OS=Erythranthe guttata GN=MIMGU_mgv1a026352mg PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.200e-10
Identity = 37/60 (61.67%), Postives = 45/60 (75.00%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYGC-FEILVPGLKLECSSEMPRLVVL 69
           RN PEL+DKPVAVCHSD+PRGT+EISSANYPARD+G    + V   K  C    P+LV++
Sbjct: 411 RNYPELLDKPVAVCHSDNPRGTAEISSANYPARDHGVKAGMFVKDAKTRC----PQLVIV 466
[Close]
BLAST of Spo24183.1 vs. UniProtKB/TrEMBL
Match: A0A059AN77_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_I010961 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 6.500e-10
Identity = 30/36 (83.33%), Postives = 34/36 (94.44%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYG 46
           RN PEL DKPVA+CHSD+P+GT+EISSANYPARDYG
Sbjct: 337 RNLPELHDKPVAICHSDNPKGTAEISSANYPARDYG 372
[Close]
BLAST of Spo24183.1 vs. UniProtKB/TrEMBL
Match: A0A0Q3H432_BRADI (DNA repair protein REV1 OS=Brachypodium distachyon GN=BRADI_1g33560 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 8.500e-10
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYG 46
           RN PEL DKPVAVCHSD+P+GT+EISSANYPARDYG
Sbjct: 397 RNRPELHDKPVAVCHSDNPKGTAEISSANYPARDYG 432
[Close]
BLAST of Spo24183.1 vs. ExPASy Swiss-Prot
Match: REV1_ARATH (DNA repair protein REV1 OS=Arabidopsis thaliana GN=REV1 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.100e-9
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYG 46
           +N  EL DKPVAVCHSD+P+GT+EISSANYPAR YG
Sbjct: 390 KNRLELHDKPVAVCHSDNPKGTAEISSANYPARAYG 425
[Close]
BLAST of Spo24183.1 vs. TAIR (Arabidopsis)
Match: AT5G44750.2 (DNA-directed DNA polymerases)

HSP 1 Score: 62.8 bits (151), Expect = 1.200e-10
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 1 

		  

Query: 10  RNCPELMDKPVAVCHSDSPRGTSEISSANYPARDYG 46
           +N  EL DKPVAVCHSD+P+GT+EISSANYPAR YG
Sbjct: 390 KNRLELHDKPVAVCHSDNPKGTAEISSANYPARAYG 425
The following BLAST results are available for this feature:
BLAST of Spo24183.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902164130|gb|KNA06932.1|1.5e-1595.3hypothetical protein SOVF_1765... [more]
gi|731360148|ref|XP_010691659.1|8.4e-1165.0PREDICTED: DNA repair protein ... [more]
gi|731360144|ref|XP_010691657.1|8.4e-1165.0PREDICTED: DNA repair protein ... [more]
gi|731360150|ref|XP_010691660.1|8.4e-1165.0PREDICTED: DNA repair protein ... [more]
gi|764524689|ref|XP_011457814.1|1.9e-1061.6PREDICTED: DNA repair protein ... [more]
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BLAST of Spo24183.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QI35_SPIOL1.0e-1595.3Uncharacterized protein OS=Spi... [more]
A0A0J8EDR7_BETVU5.9e-1165.0DNA repair protein REV1 OS=Bet... [more]
A0A022RUT3_ERYGU2.2e-1061.6DNA repair protein REV1 OS=Ery... [more]
A0A059AN77_EUCGR6.5e-1083.3Uncharacterized protein (Fragm... [more]
A0A0Q3H432_BRADI8.5e-1086.1DNA repair protein REV1 OS=Bra... [more]
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BLAST of Spo24183.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1
Match NameE-valueIdentityDescription
REV1_ARATH2.1e-977.7DNA repair protein REV1 OS=Ara... [more]
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BLAST of Spo24183.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G44750.21.2e-1077.7DNA-directed DNA polymerases[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001126UmuC domainPFAMPF00817IMScoord: 8..45
score: 1.
IPR001126UmuC domainPROFILEPS50173UMUCcoord: 1..45
score: 10
NoneNo IPR availableunknownSSF56672DNA/RNA polymerasescoord: 10..48
score: 6.2

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0006260 DNA replication
cellular_component GO:0042575 DNA polymerase complex
molecular_function GO:0003684 damaged DNA binding
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0000287 magnesium ion binding