Spo24682.1 (mRNA) | SpinachBase

Overview


Name	Spo24682.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Trypsin inhibitor 2 (SOTI II) (Trypsin inhibitor II)
Location	SpoScf_04859 : 3280 .. 4705 (+)
Sequence length	889

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTTATATTTCGTCGCCATGCATAGAAGTTATTGTATGAAAAACAATTAAAAAAGGAAAAATAGTAGTATAAATAAGAGCAATGCCATGAGTAAAAATGAGCAAAACGAATCCACTTCTTAACTATTATCAATCTTGTCGCAAAAGTGTTTAGCTAACATTATGGGTTTCTCCAAATTTGCTGTCTTTGCTCTTTTCATGCTCGCTGCATCAGGTATATATGCATGGAGTTAATTTCGTATTTTGTTAATGTATATTTTTAATTATTAAACTAGTTTTAGGCCCGGGCGATGTCCGGGTTACTACATTAATAACATTAACGTTTACTTATATATAATATTTTGTAATGATAACATGAAACATATAATAGTTTAGTGGTAAAAGCTTTATTGTTTAAACTCAGGGTCATGAGTTCCAAACTTAACAGCCTGTATCCTCTGTGTCTCAGATTAGTTGTTGTATTTTTACTTGTCGTAATATCTCCAATCTTCGAGACATGTGGTTGACCATTAATAATTAATATTTATGTACAGTATACAGTATACAATTGTGTTGTAAGACTTGTAACAAAAAAAATGGACTTTTATATTACGACTATGAAATATTAATAAGAGGAACGAAACTAAAAAGATTTAAACTAATCTGAATGGGAGGAATAAAATTCAATTATTTTTGCCACCAATAAGTAACGTTCCGTCACAGAAATTGTTGATTTGTGTAGATGTAGACTGAAATTATTTTAATTTTGCAGCAGTGATAGATGCGAAATATGAAGCAAGAACCACTGCATCGATAATGACTGAACAGCTCCTGGAAGCTGAAGTGAAGGCATTGGCATCACTTAGAGAAGTTGAAAGGGGTTTTGCTAATTCAAAGTTGAAGCAAAATCTGATGATGAGGACTACTGAAGATAAGTGTTCTCCATCAGGGGCCATATGTTCGGGTTTTGGACCTCCGGAACAATGTTGTTCTGGTGCTTGTGTTCCTCATCCTATCCTGAGGATCTTTGTGTGCCAGTAAAAGCCCGGGTCGGGTGACTCGTGACGGGTCGATCATCACCTTCAATTTCCTTTGTTAATTATGTACTCCGATTATCCGTACTTTGTAGTCTACCCGTGTACGAGATAGAATAAAATTGATGAAGGTGTTGGTGAGGAAATGCTTCTATATATGTTGTATGTCTCTTGAGACTTGAGTCTTTGTTCTTGGAATAATTAAGAATAAGTTTGGCTAGTTTCTATTAAGCCAATTATCTATATGTATGGAGTAGTTAACTTTATCGTCAATAATCCCTTTCCTTCCTATCTAAAATAGCCGAACGTATCTCCTCTTTTGCGTAATTTTTCAGTCCATATAAAAGTGCCCTTACTATTTATAGTATATATTAATTAATAGCCGTCTATAAGAAAGTATACGTGACAGTGTCG

mRNA sequence

CGTTATATTTCGTCGCCATGCATAGAAGTTATTGTATGAAAAACAATTAAAAAAGGAAAAATAGTAGTATAAATAAGAGCAATGCCATGAGTAAAAATGAGCAAAACGAATCCACTTCTTAACTATTATCAATCTTGTCGCAAAAGTGTTTAGCTAACATTATGGGTTTCTCCAAATTTGCTGTCTTTGCTCTTTTCATGCTCGCTGCATCAGCAGTGATAGATGCGAAATATGAAGCAAGAACCACTGCATCGATAATGACTGAACAGCTCCTGGAAGCTGAAGTGAAGGCATTGGCATCACTTAGAGAAGTTGAAAGGGGTTTTGCTAATTCAAAGTTGAAGCAAAATCTGATGATGAGGACTACTGAAGATAAGTGTTCTCCATCAGGGGCCATATGTTCGGGTTTTGGACCTCCGGAACAATGTTGTTCTGGTGCTTGTGTTCCTCATCCTATCCTGAGGATCTTTGTGTGCCAGTAAAAGCCCGGGTCGGGTGACTCGTGACGGGTCGATCATCACCTTCAATTTCCTTTGTTAATTATGTACTCCGATTATCCGTACTTTGTAGTCTACCCGTGTACGAGATAGAATAAAATTGATGAAGGTGTTGGTGAGGAAATGCTTCTATATATGTTGTATGTCTCTTGAGACTTGAGTCTTTGTTCTTGGAATAATTAAGAATAAGTTTGGCTAGTTTCTATTAAGCCAATTATCTATATGTATGGAGTAGTTAACTTTATCGTCAATAATCCCTTTCCTTCCTATCTAAAATAGCCGAACGTATCTCCTCTTTTGCGTAATTTTTCAGTCCATATAAAAGTGCCCTTACTATTTATAGTATATATTAATTAATAGCCGTCTATAAGAAAGTATACGTGACAGTGTCG

Coding sequence (CDS)

ATGGGTTTCTCCAAATTTGCTGTCTTTGCTCTTTTCATGCTCGCTGCATCAGCAGTGATAGATGCGAAATATGAAGCAAGAACCACTGCATCGATAATGACTGAACAGCTCCTGGAAGCTGAAGTGAAGGCATTGGCATCACTTAGAGAAGTTGAAAGGGGTTTTGCTAATTCAAAGTTGAAGCAAAATCTGATGATGAGGACTACTGAAGATAAGTGTTCTCCATCAGGGGCCATATGTTCGGGTTTTGGACCTCCGGAACAATGTTGTTCTGGTGCTTGTGTTCCTCATCCTATCCTGAGGATCTTTGTGTGCCAGTAA

Protein sequence

MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKLKQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo24682	Spo24682	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo24682.1	Spo24682.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24682.1.exon.1	Spo24682.1.exon.1	exon
Spo24682.1.exon.2	Spo24682.1.exon.2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24682.1.utr5p.1	Spo24682.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24682.1.CDS.1	Spo24682.1.CDS.1	CDS
Spo24682.1.CDS.2	Spo24682.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24682.1.utr3p.1	Spo24682.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo24682.1 vs. NCBI nr
Match: gi|902198410|gb|KNA13658.1| (hypothetical protein SOVF_114660 [Spinacia oleracea])

HSP 1 Score: 207.2 bits (526), Expect = 1.400e-50
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60
           MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL
Sbjct: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60

Query: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 106

[Close]

BLAST of Spo24682.1 vs. NCBI nr
Match: gi|902198409|gb|KNA13657.1| (hypothetical protein SOVF_114650 [Spinacia oleracea])

HSP 1 Score: 171.0 bits (432), Expect = 1.100e-39
Identity = 86/106 (81.13%), Postives = 93/106 (87.74%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60
           M FSKFAVFALFMLA S VIDAKY+ R++ SIMTE+LLEAEVKALASLREVER    SKL
Sbjct: 1   MSFSKFAVFALFMLATSGVIDAKYQPRSSGSIMTEELLEAEVKALASLREVERAVIASKL 60

Query: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           KQNLMMRTTED C  SG ICSGFGPPEQCCSGACVPHPI+++FVCQ
Sbjct: 61  KQNLMMRTTEDNCIKSGTICSGFGPPEQCCSGACVPHPIIKMFVCQ 106

[Close]

BLAST of Spo24682.1 vs. NCBI nr
Match: gi|902192504|gb|KNA12060.1| (hypothetical protein SOVF_129340 [Spinacia oleracea])

HSP 1 Score: 157.1 bits (396), Expect = 1.600e-35
Identity = 83/94 (88.30%), Postives = 88/94 (93.62%), Query Frame = 1


		  
Query: 13  MLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKLKQNLMMRTTEDK 72
           MLAASAVIDAKYEARTTASIMTE+LL+AEVKA++SLREVER  A SKLKQN+MM  TEDK
Sbjct: 1   MLAASAVIDAKYEARTTASIMTEELLKAEVKAMSSLREVERAVA-SKLKQNVMM--TEDK 60

Query: 73  CSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           CSPSG ICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 61  CSPSGNICSGFGPPEQCCSGACVPHPILRIFVCQ 91

[Close]

BLAST of Spo24682.1 vs. NCBI nr
Match: gi|902198411|gb|KNA13659.1| (hypothetical protein SOVF_114670 [Spinacia oleracea])

HSP 1 Score: 129.8 bits (325), Expect = 2.800e-27
Identity = 73/106 (68.87%), Postives = 80/106 (75.47%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60
           MGFSKFAVFAL MLAAS VIDAKY+  TT  IM+E+LL+AEVKALASLRE+ER    SKL
Sbjct: 1   MGFSKFAVFALLMLAASGVIDAKYQPITTGPIMSEELLKAEVKALASLREIERSVIASKL 60

Query: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
            Q L+   TEDKCSP GA C   G  E CCSGACVPHP + I VCQ
Sbjct: 61  MQKLI--RTEDKCSPPGAAC---GRSEDCCSGACVPHPFMFIIVCQ 101

[Close]

BLAST of Spo24682.1 vs. NCBI nr
Match: gi|731335337|ref|XP_010678676.1| (PREDICTED: uncharacterized protein LOC104894196 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 109.8 bits (273), Expect = 3.000e-21
Identity = 61/107 (57.01%), Postives = 78/107 (72.90%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVI--DAKYEARTTASIMTEQLLEAEVKALASLREVERGFANS 60
           + + K AV A FML ASA+    AK +  T+   ++  LLEAE+KAL +L++VE+  + S
Sbjct: 2   LSYYKVAVVAFFMLTASAMAMTGAKIQPITSTQTVSNGLLEAEIKALTALKQVEKAIS-S 61

Query: 61  KLKQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVC 106
            LKQ LM    E++C+PSG ICSGFGPPEQCCSGACVPHP+LRIFVC
Sbjct: 62  DLKQKLM---GEEECAPSGHICSGFGPPEQCCSGACVPHPMLRIFVC 104

[Close]

BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R2C8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_114660 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 9.700e-51
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60
           MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL
Sbjct: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60

Query: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 106

[Close]

BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R4D4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_114650 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 7.700e-40
Identity = 86/106 (81.13%), Postives = 93/106 (87.74%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60
           M FSKFAVFALFMLA S VIDAKY+ R++ SIMTE+LLEAEVKALASLREVER    SKL
Sbjct: 1   MSFSKFAVFALFMLATSGVIDAKYQPRSSGSIMTEELLEAEVKALASLREVERAVIASKL 60

Query: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           KQNLMMRTTED C  SG ICSGFGPPEQCCSGACVPHPI+++FVCQ
Sbjct: 61  KQNLMMRTTEDNCIKSGTICSGFGPPEQCCSGACVPHPIIKMFVCQ 106

[Close]

BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QXT1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_129340 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.100e-35
Identity = 83/94 (88.30%), Postives = 88/94 (93.62%), Query Frame = 1


		  
Query: 13  MLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKLKQNLMMRTTEDK 72
           MLAASAVIDAKYEARTTASIMTE+LL+AEVKA++SLREVER  A SKLKQN+MM  TEDK
Sbjct: 1   MLAASAVIDAKYEARTTASIMTEELLKAEVKAMSSLREVERAVA-SKLKQNVMM--TEDK 60

Query: 73  CSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           CSPSG ICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 61  CSPSGNICSGFGPPEQCCSGACVPHPILRIFVCQ 91

[Close]

BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R400_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_114670 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.000e-27
Identity = 73/106 (68.87%), Postives = 80/106 (75.47%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVIDAKYEARTTASIMTEQLLEAEVKALASLREVERGFANSKL 60
           MGFSKFAVFAL MLAAS VIDAKY+  TT  IM+E+LL+AEVKALASLRE+ER    SKL
Sbjct: 1   MGFSKFAVFALLMLAASGVIDAKYQPITTGPIMSEELLKAEVKALASLREIERSVIASKL 60

Query: 61  KQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
            Q L+   TEDKCSP GA C   G  E CCSGACVPHP + I VCQ
Sbjct: 61  MQKLI--RTEDKCSPPGAAC---GRSEDCCSGACVPHPFMFIIVCQ 101

[Close]

BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CER6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g116170 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.100e-21
Identity = 61/107 (57.01%), Postives = 78/107 (72.90%), Query Frame = 1


		  
Query: 1   MGFSKFAVFALFMLAASAVI--DAKYEARTTASIMTEQLLEAEVKALASLREVERGFANS 60
           + + K AV A FML ASA+    AK +  T+   ++  LLEAE+KAL +L++VE+  + S
Sbjct: 2   LSYYKVAVVAFFMLTASAMAMTGAKIQPITSTQTVSNGLLEAEIKALTALKQVEKAIS-S 61

Query: 61  KLKQNLMMRTTEDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVC 106
            LKQ LM    E++C+PSG ICSGFGPPEQCCSGACVPHP+LRIFVC
Sbjct: 62  DLKQKLM---GEEECAPSGHICSGFGPPEQCCSGACVPHPMLRIFVC 104

[Close]

BLAST of Spo24682.1 vs. ExPASy Swiss-Prot
Match: ITR3_SPIOL (Trypsin inhibitor 3 OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.700e-17
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 1


		  
Query: 70  EDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           EDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 1   EDKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 37

[Close]

BLAST of Spo24682.1 vs. ExPASy Swiss-Prot
Match: ITR2_SPIOL (Trypsin inhibitor 2 OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.900e-16
Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 1


		  
Query: 71  DKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           DKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 1   DKCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 36

[Close]

BLAST of Spo24682.1 vs. ExPASy Swiss-Prot
Match: ITR1_SPIOL (Trypsin inhibitor 1 OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.500e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1


		  
Query: 72  KCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 107
           KCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ
Sbjct: 1   KCSPSGAICSGFGPPEQCCSGACVPHPILRIFVCQ 35

The following BLAST results are available for this feature:

BLAST of Spo24682.1 vs. NCBI nr
BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
BLAST of Spo24682.1 vs. ExPASy Swiss-Prot
BLAST of Spo24682.1 vs. TAIR (Arabidopsis)

BLAST of Spo24682.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902198410\|gb\|KNA13658.1\|	1.4e-50	100.	hypothetical protein SOVF_1146...	[more]
gi\|902198409\|gb\|KNA13657.1\|	1.1e-39	81.1	hypothetical protein SOVF_1146...	[more]
gi\|902192504\|gb\|KNA12060.1\|	1.6e-35	88.3	hypothetical protein SOVF_1293...	[more]
gi\|902198411\|gb\|KNA13659.1\|	2.8e-27	68.8	hypothetical protein SOVF_1146...	[more]
gi\|731335337\|ref\|XP_010678676.1\|	3.0e-21	57.0	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo24682.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R2C8_SPIOL	9.7e-51	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9R4D4_SPIOL	7.7e-40	81.1	Uncharacterized protein OS=Spi...	[more]
A0A0K9QXT1_SPIOL	1.1e-35	88.3	Uncharacterized protein OS=Spi...	[more]
A0A0K9R400_SPIOL	2.0e-27	68.8	Uncharacterized protein OS=Spi...	[more]
A0A0J8CER6_BETVU	2.1e-21	57.0	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo24682.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 3


Match Name	E-value	Identity	Description
ITR3_SPIOL	7.7e-17	100.	Trypsin inhibitor 3 OS=Spinaci...	[more]
ITR2_SPIOL	2.9e-16	100.	Trypsin inhibitor 2 OS=Spinaci...	[more]
ITR1_SPIOL	1.5e-15	100.	Trypsin inhibitor 1 OS=Spinaci...	[more]

BLAST of Spo24682.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity