Spo24754.1 (mRNA) | SpinachBase

Overview


Name	Spo24754.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Histone H2A
Location	SpoScf_00257 : 378859 .. 380236 (-)
Sequence length	941

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCCTTTCAACCAATCAAAATGCAGGATATCCCCTTATATAAACCAAACTCATCTCTCTCACCATAAACCCTGATTTCCCTGTCACACCAACAACGCACTATCTTCCTCCTACCTATTTCTCTCGATCACTTTCTCTCTCCTAGGGTTCTGATCATCGTTAGATTTCCGGCATAAAAATGAGCGGTAAAGAAGGCGGCGGATCCACCAAGGGAGGCAGAGGAAAGCCTAAAGCTTCAAAATCGGTTTCGAGGTCTTCCAAAGCCGGTCTGCAGTTCCCCGTCGGTAGGATCGCTAGATTTTTGAAGGCCGGAAAGTATGCTGAGCGTGTTGGTGCTGGTGCTCCAGTCTATCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTACCTCACTTTCTCCCTTTTTCGAGTTTATGCTAAATCAATTAAATCTGCGGAAGTCTGTAGATCGATTTTGATTTCGTTTTTTTTGTTTGGCGTTGATTGATTATTTGATTTTAAATGAAATTTCAAGATCTAATTTCTCATGATTTGCTGTTGATTGATGTTCAATTAGCAGTCCTGATGAATTAACTTAGTTGCATAATAGCCGAAGTGTCTGATTTTAATTCATAGCCTCTGTTTAATTCTCAATCCATCATTTTCCAATTGATTGAAATATGTATGTTTCTCTGATTTCATAATAATATTACAACACTTGTTTTATATCAATCAATTGCAACGATCATAGATGATTATAGTGGCATTTAATTTCAGTTATGTCAATCTTTATGAAATTAGTGACTGTAGGGTTTGTTCTAATTAATTGAAAATGTTAACAGGTATTGGAGTTGGCTGGAAACGCAGCAAGAGACAACAAGAAGTCAAGAATAGTACCGCGTCACATTCAATTAGCAGTGAGGAACGATGAGGAATTAAGCAAGCTTTTGGGTCAAGTAACAATCGCAAACGGCGGGGTATTGCCCAACATTAATCCGACTCTTCTGCCCAGGAAGCTAGCGGGCGGTGGTAAAGACAATAATACTCTATCTCAAGAGTTCTAGACATGTTTCGGATGTACTTTAGAAAGAGTTATAATTAGTGCTTATTTTCAATGGAGTTATGGGTTTGATTATTCCCCTTTTTTTGTTCTTAATATAGCAGGGGTTATAATGTTGGTGTAATGTAGTAGTCCGTGGAATTTGATAAGCTTCTTTTATATGGAAAATTTGTTAAAATTCGCGCATATTTTTTTTGTTGGTGTCACTGACTCACTTTGAAAGCATATTCTGAAATTATTCAATGACTGTCACTGTAATTTTTTTTTTTGGTTAAAATTTACTGTAATTAGTTGATGTTGGGATGATATGAAATAATGATCAAAAGGTTCATTTGCAG

mRNA sequence

CTCCCTTTCAACCAATCAAAATGCAGGATATCCCCTTATATAAACCAAACTCATCTCTCTCACCATAAACCCTGATTTCCCTGTCACACCAACAACGCACTATCTTCCTCCTACCTATTTCTCTCGATCACTTTCTCTCTCCTAGGGTTCTGATCATCGTTAGATTTCCGGCATAAAAATGAGCGGTAAAGAAGGCGGCGGATCCACCAAGGGAGGCAGAGGAAAGCCTAAAGCTTCAAAATCGGTTTCGAGGTCTTCCAAAGCCGGTCTGCAGTTCCCCGTCGGTAGGATCGCTAGATTTTTGAAGGCCGGAAAGTATGCTGAGCGTGTTGGTGCTGGTGCTCCAGTCTATCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTATTGGAGTTGGCTGGAAACGCAGCAAGAGACAACAAGAAGTCAAGAATAGTACCGCGTCACATTCAATTAGCAGTGAGGAACGATGAGGAATTAAGCAAGCTTTTGGGTCAAGTAACAATCGCAAACGGCGGGGTATTGCCCAACATTAATCCGACTCTTCTGCCCAGGAAGCTAGCGGGCGGTGGTAAAGACAATAATACTCTATCTCAAGAGTTCTAGACATGTTTCGGATGTACTTTAGAAAGAGTTATAATTAGTGCTTATTTTCAATGGAGTTATGGGTTTGATTATTCCCCTTTTTTTGTTCTTAATATAGCAGGGGTTATAATGTTGGTGTAATGTAGTAGTCCGTGGAATTTGATAAGCTTCTTTTATATGGAAAATTTGTTAAAATTCGCGCATATTTTTTTTGTTGGTGTCACTGACTCACTTTGAAAGCATATTCTGAAATTATTCAATGACTGTCACTGTAATTTTTTTTTTTGGTTAAAATTTACTGTAATTAGTTGATGTTGGGATGATATGAAATAATGATCAAAAGGTTCATTTGCAG

Coding sequence (CDS)

ATGAGCGGTAAAGAAGGCGGCGGATCCACCAAGGGAGGCAGAGGAAAGCCTAAAGCTTCAAAATCGGTTTCGAGGTCTTCCAAAGCCGGTCTGCAGTTCCCCGTCGGTAGGATCGCTAGATTTTTGAAGGCCGGAAAGTATGCTGAGCGTGTTGGTGCTGGTGCTCCAGTCTATCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTATTGGAGTTGGCTGGAAACGCAGCAAGAGACAACAAGAAGTCAAGAATAGTACCGCGTCACATTCAATTAGCAGTGAGGAACGATGAGGAATTAAGCAAGCTTTTGGGTCAAGTAACAATCGCAAACGGCGGGGTATTGCCCAACATTAATCCGACTCTTCTGCCCAGGAAGCTAGCGGGCGGTGGTAAAGACAATAATACTCTATCTCAAGAGTTCTAG

Protein sequence

MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPRKLAGGGKDNNTLSQEF

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo24754	Spo24754	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo24754.1	Spo24754.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24754.1.utr5p.1	Spo24754.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24754.1.CDS.2	Spo24754.1.CDS.2	CDS
Spo24754.1.CDS.1	Spo24754.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24754.1.utr3p.1	Spo24754.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo24754.1.exon.2	Spo24754.1.exon.2	exon
Spo24754.1.exon.1	Spo24754.1.exon.1	exon

Homology

[Close]

BLAST of Spo24754.1 vs. NCBI nr
Match: gi|902174491|gb|KNA08418.1| (hypothetical protein SOVF_162780 [Spinacia oleracea])

HSP 1 Score: 268.5 bits (685), Expect = 6.800e-69
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
           PTLLPRKLAGGGKDNNTLSQEF
Sbjct: 121 PTLLPRKLAGGGKDNNTLSQEF 142

[Close]

BLAST of Spo24754.1 vs. NCBI nr
Match: gi|743910741|ref|XP_011048890.1| (PREDICTED: histone H2AX [Populus euphratica])

HSP 1 Score: 232.3 bits (591), Expect = 5.400e-58
Identity = 125/142 (88.03%), Postives = 130/142 (91.55%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS KEGG STKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSKEGGASTKGGRGKPKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP+K+  G  D  + SQEF
Sbjct: 121 QTLLPKKIGKGKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. NCBI nr
Match: gi|743829393|ref|XP_011023506.1| (PREDICTED: histone H2AX-like [Populus euphratica])

HSP 1 Score: 232.3 bits (591), Expect = 5.400e-58
Identity = 125/142 (88.03%), Postives = 130/142 (91.55%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS KEGG STKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSKEGGASTKGGRGKPKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP+K+  G  D  + SQEF
Sbjct: 121 QTLLPKKMGKGKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. NCBI nr
Match: gi|255542492|ref|XP_002512309.1| (PREDICTED: histone H2AX [Ricinus communis])

HSP 1 Score: 231.9 bits (590), Expect = 7.100e-58
Identity = 123/136 (90.44%), Postives = 128/136 (94.12%), Query Frame = 1


		  
Query: 7   GGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYL 66
           GGSTKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYL
Sbjct: 5   GGSTKGGRGKPKASKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYL 64

Query: 67  AAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPR 126
           AAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+ TLLP+
Sbjct: 65  AAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPK 124

Query: 127 KLAGGGKDNNTLSQEF 143
           K+  G  D N+LSQEF
Sbjct: 125 KIGKGKGDMNSLSQEF 140

[Close]

BLAST of Spo24754.1 vs. NCBI nr
Match: gi|566169383|ref|XP_006382663.1| (histone H2A family protein [Populus trichocarpa])

HSP 1 Score: 231.9 bits (590), Expect = 7.100e-58
Identity = 125/142 (88.03%), Postives = 130/142 (91.55%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS KEGG STKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSKEGGASTKGGRGKPKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP+K+  G  D  + SQEF
Sbjct: 121 QTLLPKKVGKGKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QP00_SPIOL (Histone H2A OS=Spinacia oleracea GN=SOVF_162780 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 4.700e-69
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
           PTLLPRKLAGGGKDNNTLSQEF
Sbjct: 121 PTLLPRKLAGGGKDNNTLSQEF 142

[Close]

BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
Match: B9MZP0_POPTR (Histone H2A OS=Populus trichocarpa GN=POPTR_0005s04260g PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 4.900e-58
Identity = 125/142 (88.03%), Postives = 130/142 (91.55%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS KEGG STKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSKEGGASTKGGRGKPKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP+K+  G  D  + SQEF
Sbjct: 121 QTLLPKKVGKGKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
Match: B9REZ0_RICCO (Histone H2A OS=Ricinus communis GN=RCOM_1429760 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 4.900e-58
Identity = 123/136 (90.44%), Postives = 128/136 (94.12%), Query Frame = 1


		  
Query: 7   GGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYL 66
           GGSTKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYL
Sbjct: 5   GGSTKGGRGKPKASKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYL 64

Query: 67  AAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPR 126
           AAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+ TLLP+
Sbjct: 65  AAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPK 124

Query: 127 KLAGGGKDNNTLSQEF 143
           K+  G  D N+LSQEF
Sbjct: 125 KIGKGKGDMNSLSQEF 140

[Close]

BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
Match: B9I828_POPTR (Histone H2A OS=Populus trichocarpa GN=POPTR_0013s02990g PE=3 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 6.400e-58
Identity = 124/142 (87.32%), Postives = 130/142 (91.55%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS KEGG STKGGRGKPKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+
Sbjct: 44  MSSKEGGASTKGGRGKPKASKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLA 103

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 104 AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 163

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP+K+  G  D  + SQEF
Sbjct: 164 QTLLPKKMGKGKGDIGSASQEF 185

[Close]

BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
Match: A0A059B728_EUCGR (Histone H2A OS=Eucalyptus grandis GN=EUGRSUZ_H04433 PE=3 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 2.400e-57
Identity = 123/142 (86.62%), Postives = 129/142 (90.85%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS KEGGG  KGGRGKPK+SKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSKEGGGGGKGGRGKPKSSKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP+K+  G  D  + SQEF
Sbjct: 121 QTLLPKKMGKGKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
Match: H2AXB_ARATH (Probable histone H2AXb OS=Arabidopsis thaliana GN=At1g54690 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 2.100e-57
Identity = 121/142 (85.21%), Postives = 127/142 (89.44%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS   G G+TKGGRGKPKA+KSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSGAGSGTTKGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP K+     D  + SQEF
Sbjct: 121 QTLLPSKVGKNKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
Match: H2AXA_ARATH (Probable histone H2AXa OS=Arabidopsis thaliana GN=At1g08880 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 7.800e-57
Identity = 120/142 (84.51%), Postives = 127/142 (89.44%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS   G G+TKGGRGKPKA+KSVSRSSKAGLQFPVGRIARFLK+GKYAERVGAGAPVYLS
Sbjct: 1   MSTGAGSGTTKGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKSGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP K+     D  + SQEF
Sbjct: 121 QTLLPSKVGKNKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
Match: H2AX_CICAR (Histone H2AX OS=Cicer arietinum GN=HIS2A PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.300e-56
Identity = 117/134 (87.31%), Postives = 124/134 (92.54%), Query Frame = 1


		  
Query: 9   STKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 68
           +TKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA
Sbjct: 6   TTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 65

Query: 69  EVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPRKL 128
           EVLELAGNAARDNK +RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+ TLLP+K+
Sbjct: 66  EVLELAGNAARDNKNNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKV 125

Query: 129 AGGGKDNNTLSQEF 143
             G  +  + SQEF
Sbjct: 126 GKGKGEIGSASQEF 139

[Close]

BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
Match: H2AX_PICAB (Histone H2AX OS=Picea abies PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 2.600e-52
Identity = 110/132 (83.33%), Postives = 118/132 (89.39%), Query Frame = 1


		  
Query: 11  KGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 70
           KGGRGK K++KSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEYLAAEV
Sbjct: 7   KGGRGKAKSTKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAEV 66

Query: 71  LELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPRKLAG 130
           LELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+  LLP+K   
Sbjct: 67  LELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIHQVLLPKKSGK 126

Query: 131 GGKDNNTLSQEF 143
              +  + SQEF
Sbjct: 127 DKGEIGSASQEF 138

[Close]

BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
Match: H2AXB_ORYSI (Probable histone H2AXb OS=Oryza sativa subsp. indica GN=OsI_037312 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 3.400e-52
Identity = 117/140 (83.57%), Postives = 123/140 (87.86%), Query Frame = 1


		  
Query: 6   GGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEY 65
           GGG   GGRGK K SKSVSRSSKAGLQFPVGRIAR+LKAGKYAERVGAGAPVYLSAVLEY
Sbjct: 5   GGG---GGRGKSKGSKSVSRSSKAGLQFPVGRIARYLKAGKYAERVGAGAPVYLSAVLEY 64

Query: 66  LAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLP 125
           LAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELS+LLG VTIA GGVLPNI+ TLLP
Sbjct: 65  LAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSRLLGAVTIAAGGVLPNIHQTLLP 124

Query: 126 RKLAGGGKDN---NTLSQEF 143
           +K   GGKD     + SQEF
Sbjct: 125 KK---GGKDKADIGSASQEF 138

[Close]

BLAST of Spo24754.1 vs. TAIR (Arabidopsis)
Match: AT1G54690.1 (gamma histone variant H2AX)

HSP 1 Score: 223.0 bits (567), Expect = 1.200e-58
Identity = 121/142 (85.21%), Postives = 127/142 (89.44%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS   G G+TKGGRGKPKA+KSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS
Sbjct: 1   MSSGAGSGTTKGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP K+     D  + SQEF
Sbjct: 121 QTLLPSKVGKNKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. TAIR (Arabidopsis)
Match: AT1G08880.1 (Histone superfamily protein)

HSP 1 Score: 221.1 bits (562), Expect = 4.400e-58
Identity = 120/142 (84.51%), Postives = 127/142 (89.44%), Query Frame = 1


		  
Query: 1   MSGKEGGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 60
           MS   G G+TKGGRGKPKA+KSVSRSSKAGLQFPVGRIARFLK+GKYAERVGAGAPVYLS
Sbjct: 1   MSTGAGSGTTKGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKSGKYAERVGAGAPVYLS 60

Query: 61  AVLEYLAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNIN 120
           AVLEYLAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNI+
Sbjct: 61  AVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIH 120

Query: 121 PTLLPRKLAGGGKDNNTLSQEF 143
            TLLP K+     D  + SQEF
Sbjct: 121 QTLLPSKVGKNKGDIGSASQEF 142

[Close]

BLAST of Spo24754.1 vs. TAIR (Arabidopsis)
Match: AT4G27230.1 (histone H2A 2)

HSP 1 Score: 191.0 bits (484), Expect = 4.900e-49
Identity = 106/125 (84.80%), Postives = 111/125 (88.80%), Query Frame = 1


		  
Query: 13  GRGKP----KASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 72
           GRGK      A KS SRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEYLAA
Sbjct: 3   GRGKQLGSGAAKKSTSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAA 62

Query: 73  EVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPRKL 132
           EVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGV+PNI+  LLP+K 
Sbjct: 63  EVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKK- 122

Query: 133 AGGGK 134
           AG  K
Sbjct: 123 AGSSK 126

[Close]

BLAST of Spo24754.1 vs. TAIR (Arabidopsis)
Match: AT5G54640.1 (Histone superfamily protein)

HSP 1 Score: 188.7 bits (478), Expect = 2.400e-48
Identity = 106/128 (82.81%), Postives = 112/128 (87.50%), Query Frame = 1


		  
Query: 6   GGGSTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEY 65
           G G T G  G   A K+ SRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEY
Sbjct: 3   GRGKTLGSGG---AKKATSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEY 62

Query: 66  LAAEVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLP 125
           LAAEVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGV+PNI+  LLP
Sbjct: 63  LAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLP 122

Query: 126 RKLAGGGK 134
           +K AG  K
Sbjct: 123 KK-AGASK 126

[Close]

BLAST of Spo24754.1 vs. TAIR (Arabidopsis)
Match: AT1G51060.1 (histone H2A 10)

HSP 1 Score: 186.4 bits (472), Expect = 1.200e-47
Identity = 100/119 (84.03%), Postives = 107/119 (89.92%), Query Frame = 1


		  
Query: 13  GRGKP----KASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 72
           GRGK      A K+ +RSSKAGLQFPVGRIARFLK GKYAERVGAGAPVYL+AVLEYLAA
Sbjct: 3   GRGKTLGSGSAKKATTRSSKAGLQFPVGRIARFLKKGKYAERVGAGAPVYLAAVLEYLAA 62

Query: 73  EVLELAGNAARDNKKSRIVPRHIQLAVRNDEELSKLLGQVTIANGGVLPNINPTLLPRK 128
           EVLELAGNAARDNKK+RIVPRHIQLAVRNDEELSKLLG VTIANGGV+PNI+  LLP+K
Sbjct: 63  EVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKK 121

The following BLAST results are available for this feature:

BLAST of Spo24754.1 vs. NCBI nr
BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
BLAST of Spo24754.1 vs. TAIR (Arabidopsis)

BLAST of Spo24754.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902174491\|gb\|KNA08418.1\|	6.8e-69	100.	hypothetical protein SOVF_1627...	[more]
gi\|743910741\|ref\|XP_011048890.1\|	5.4e-58	88.0	PREDICTED: histone H2AX [Popul...	[more]
gi\|743829393\|ref\|XP_011023506.1\|	5.4e-58	88.0	PREDICTED: histone H2AX-like [...	[more]
gi\|255542492\|ref\|XP_002512309.1\|	7.1e-58	90.4	PREDICTED: histone H2AX [Ricin...	[more]
gi\|566169383\|ref\|XP_006382663.1\|	7.1e-58	88.0	histone H2A family protein [Po...	[more]

BLAST of Spo24754.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QP00_SPIOL	4.7e-69	100.	Histone H2A OS=Spinacia olerac...	[more]
B9MZP0_POPTR	4.9e-58	88.0	Histone H2A OS=Populus trichoc...	[more]
B9REZ0_RICCO	4.9e-58	90.4	Histone H2A OS=Ricinus communi...	[more]
B9I828_POPTR	6.4e-58	87.3	Histone H2A OS=Populus trichoc...	[more]
A0A059B728_EUCGR	2.4e-57	86.6	Histone H2A OS=Eucalyptus gran...	[more]

BLAST of Spo24754.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
H2AXB_ARATH	2.1e-57	85.2	Probable histone H2AXb OS=Arab...	[more]
H2AXA_ARATH	7.8e-57	84.5	Probable histone H2AXa OS=Arab...	[more]
H2AX_CICAR	1.3e-56	87.3	Histone H2AX OS=Cicer arietinu...	[more]
H2AX_PICAB	2.6e-52	83.3	Histone H2AX OS=Picea abies PE...	[more]
H2AXB_ORYSI	3.4e-52	83.5	Probable histone H2AXb OS=Oryz...	[more]

BLAST of Spo24754.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G54690.1	1.2e-58	85.2	gamma histone variant H2AX	[more]
AT1G08880.1	4.4e-58	84.5	Histone superfamily protein	[more]
AT4G27230.1	4.9e-49	84.8	histone H2A 2	[more]
AT5G54640.1	2.4e-48	82.8	Histone superfamily protein	[more]
AT1G51060.1	1.2e-47	84.0	histone H2A 10	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002119	Histone H2A	PRINTS	PR00620	HISTONEH2A	coord: 107..125 score: 2.0E-52coord: 65..78 score: 2.0E-52coord: 79..93 score: 2.0E-52coord: 21..43 score: 2.0E-52coord: 50..65 score: 2.0
IPR002119	Histone H2A	SMART	SM00414	h2a4	coord: 10..130 score: 6.1
IPR007125	Histone H2A/H2B/H3	PFAM	PF00125	Histone	coord: 17..96 score: 2.0
IPR009072	Histone-fold	GENE3D	1.10.20.10		coord: 14..127 score: 1.3
IPR009072	Histone-fold	unknown	SSF47113	Histone-fold	coord: 14..131 score: 3.29
None	No IPR available	PANTHER	PTHR23430	HISTONE H2A	coord: 7..142 score: 4.8E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0000786	nucleosome
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046982	protein heterodimerization activity