Spo25105.1 (mRNA) | SpinachBase

Overview


Name	Spo25105.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	High affinity nitrate transporter
Location	chr4 : 4877366 .. 4878349 (+)
Sequence length	984

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGTTTCTTTATAGCTTTATCCTTAATAAAACACCGGCCGACACGCCGTTCACGGCGTGGAGGGTGGCGTTTTTCATCCCCGCCGGCCTACAAATGTTTTCGGCTTATGCAGTCATGCTTTTCGGGCAGGATCTTCCTGACGGAAACTTCCTGCAAATGCAGAAATCTGGTGCAATGCACAAAGACAAAGCTTCCAAGATTGTATACCATGCAGTCACAAATTACAGGAGCTGGATTACTGCTATAGGGTACGGGTTCTGTTTCGGGGTTGAGCTAGCCGTAACAAACGTTGTTGTTTACTACTTCTATGACAGATTCAACCTGAACCTGAACCTAGCAGGGATTGTTGGTGCTCTTTTCGGGATGGTTAACATCTTCTCCAGGTCTTCCGGTGGTTGGTTTTCCGATTTCATGGCGAAGAAGTTCGGGATGAGGGGTAGATTATGGGGTTGGTGGACTGTGCAGACACTAAGTGGTGTAATGTGTATCGTTATGGGACGCATGAACACTTTAAGCTCCACCATTGCTGTTATGATGATCTTCTCCTTTTTAGTTCAAGCAGCTGAAGGACTCACATTCGGGGTCGTTCCTTTTGTCTCCAGAAGATCATTGGGCGTGGTTTCGGGATTGTCTGGAGCAGGTGGAAATGCGGGTGCAGTGATATGTCAAGTGATTTTCTTTAGAGGATCACAATTCAAAATAGAACACGGGTTAACTTACATGGGTATTGTGATCATAGCATGTACCCTTAGCTTAGGTACCATAAACTTCCCACAGTGGGGCGGTATGTTTTGCGGTCCAAAACCAAATGTCACAGAAGAAGACTACTACATGAGGGAATGGGATGCTGAAGAGCAGGAGCAAGGGCTTCATAGGGTAAGCATGAAGTTCGCGGAAGGCAGCCGGAATGAGAGAAGCTCGAAAGTAATTTCTGTTACAGAAAAAGAAGATAGCACACCAACACCAGTTAATAATGTCTAG

mRNA sequence

ATGCCGTTTCTTTATAGCTTTATCCTTAATAAAACACCGGCCGACACGCCGTTCACGGCGTGGAGGGTGGCGTTTTTCATCCCCGCCGGCCTACAAATGTTTTCGGCTTATGCAGTCATGCTTTTCGGGCAGGATCTTCCTGACGGAAACTTCCTGCAAATGCAGAAATCTGGTGCAATGCACAAAGACAAAGCTTCCAAGATTGTATACCATGCAGTCACAAATTACAGGAGCTGGATTACTGCTATAGGGTACGGGTTCTGTTTCGGGGTTGAGCTAGCCGTAACAAACGTTGTTGTTTACTACTTCTATGACAGATTCAACCTGAACCTGAACCTAGCAGGGATTGTTGGTGCTCTTTTCGGGATGGTTAACATCTTCTCCAGGTCTTCCGGTGGTTGGTTTTCCGATTTCATGGCGAAGAAGTTCGGGATGAGGGGTAGATTATGGGGTTGGTGGACTGTGCAGACACTAAGTGGTGTAATGTGTATCGTTATGGGACGCATGAACACTTTAAGCTCCACCATTGCTGTTATGATGATCTTCTCCTTTTTAGTTCAAGCAGCTGAAGGACTCACATTCGGGGTCGTTCCTTTTGTCTCCAGAAGATCATTGGGCGTGGTTTCGGGATTGTCTGGAGCAGGTGGAAATGCGGGTGCAGTGATATGTCAAGTGATTTTCTTTAGAGGATCACAATTCAAAATAGAACACGGGTTAACTTACATGGGTATTGTGATCATAGCATGTACCCTTAGCTTAGGTACCATAAACTTCCCACAGTGGGGCGGTATGTTTTGCGGTCCAAAACCAAATGTCACAGAAGAAGACTACTACATGAGGGAATGGGATGCTGAAGAGCAGGAGCAAGGGCTTCATAGGGTAAGCATGAAGTTCGCGGAAGGCAGCCGGAATGAGAGAAGCTCGAAAGTAATTTCTGTTACAGAAAAAGAAGATAGCACACCAACACCAGTTAATAATGTCTAG

Coding sequence (CDS)

ATGCCGTTTCTTTATAGCTTTATCCTTAATAAAACACCGGCCGACACGCCGTTCACGGCGTGGAGGGTGGCGTTTTTCATCCCCGCCGGCCTACAAATGTTTTCGGCTTATGCAGTCATGCTTTTCGGGCAGGATCTTCCTGACGGAAACTTCCTGCAAATGCAGAAATCTGGTGCAATGCACAAAGACAAAGCTTCCAAGATTGTATACCATGCAGTCACAAATTACAGGAGCTGGATTACTGCTATAGGGTACGGGTTCTGTTTCGGGGTTGAGCTAGCCGTAACAAACGTTGTTGTTTACTACTTCTATGACAGATTCAACCTGAACCTGAACCTAGCAGGGATTGTTGGTGCTCTTTTCGGGATGGTTAACATCTTCTCCAGGTCTTCCGGTGGTTGGTTTTCCGATTTCATGGCGAAGAAGTTCGGGATGAGGGGTAGATTATGGGGTTGGTGGACTGTGCAGACACTAAGTGGTGTAATGTGTATCGTTATGGGACGCATGAACACTTTAAGCTCCACCATTGCTGTTATGATGATCTTCTCCTTTTTAGTTCAAGCAGCTGAAGGACTCACATTCGGGGTCGTTCCTTTTGTCTCCAGAAGATCATTGGGCGTGGTTTCGGGATTGTCTGGAGCAGGTGGAAATGCGGGTGCAGTGATATGTCAAGTGATTTTCTTTAGAGGATCACAATTCAAAATAGAACACGGGTTAACTTACATGGGTATTGTGATCATAGCATGTACCCTTAGCTTAGGTACCATAAACTTCCCACAGTGGGGCGGTATGTTTTGCGGTCCAAAACCAAATGTCACAGAAGAAGACTACTACATGAGGGAATGGGATGCTGAAGAGCAGGAGCAAGGGCTTCATAGGGTAAGCATGAAGTTCGCGGAAGGCAGCCGGAATGAGAGAAGCTCGAAAGTAATTTCTGTTACAGAAAAAGAAGATAGCACACCAACACCAGTTAATAATGTCTAG

Protein sequence

MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAMHKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGALFGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMMIFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLTYMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAEGSRNERSSKVISVTEKEDSTPTPVNNV

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo25105	Spo25105	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo25105.1	Spo25105.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25105.1.exon.1	Spo25105.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25105.1.CDS.1	Spo25105.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo25105.1 vs. NCBI nr
Match: gi|902038373|gb|KNA02718.1| (hypothetical protein SOVF_216030 [Spinacia oleracea])

HSP 1 Score: 653.3 bits (1684), Expect = 2.300e-184
Identity = 326/327 (99.69%), Postives = 327/327 (100.00%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM
Sbjct: 157 MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 216

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL
Sbjct: 217 HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 276

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM
Sbjct: 277 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 336

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT
Sbjct: 337 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 396

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE
Sbjct: 397 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 456

Query: 301 GSRNERSSKVISVTEKEDSTPTPVNNV 328
           GSRNERSSKVISVTE+EDSTPTPVNNV
Sbjct: 457 GSRNERSSKVISVTEEEDSTPTPVNNV 483

[Close]

BLAST of Spo25105.1 vs. NCBI nr
Match: gi|166209891|gb|ABL89187.3| (high affinity nitrate transporter [Spinacia oleracea])

HSP 1 Score: 651.4 bits (1679), Expect = 8.600e-184
Identity = 325/327 (99.39%), Postives = 326/327 (99.69%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM
Sbjct: 182 MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 241

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL
Sbjct: 242 HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 301

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSST AVMM
Sbjct: 302 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTTAVMM 361

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT
Sbjct: 362 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 421

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE
Sbjct: 422 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 481

Query: 301 GSRNERSSKVISVTEKEDSTPTPVNNV 328
           GSRNERSSKVISVTE+EDSTPTPVNNV
Sbjct: 482 GSRNERSSKVISVTEEEDSTPTPVNNV 508

[Close]

BLAST of Spo25105.1 vs. NCBI nr
Match: gi|902216966|gb|KNA17572.1| (hypothetical protein SOVF_078330 [Spinacia oleracea])

HSP 1 Score: 577.8 bits (1488), Expect = 1.200e-161
Identity = 287/322 (89.13%), Postives = 305/322 (94.72%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP LYS ILNKTPA TPFTAWRVAFFIPA LQMFSA+AVMLFGQDLPDGNFLQMQKSG M
Sbjct: 190 MPLLYSLILNKTPASTPFTAWRVAFFIPAFLQMFSAFAVMLFGQDLPDGNFLQMQKSGEM 249

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDKASK+ YHAVTNYRSWITAIGYGFCFGVELAVTN++V+YFYDRFNLNLNLAGIVGAL
Sbjct: 250 HKDKASKVFYHAVTNYRSWITAIGYGFCFGVELAVTNIIVFYFYDRFNLNLNLAGIVGAL 309

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVNIFSRSSGGWFSDFMAKK+G+RGRLWGWW VQTLSGVMCIVMGR++TLS+TIAVMM
Sbjct: 310 FGMVNIFSRSSGGWFSDFMAKKYGIRGRLWGWWGVQTLSGVMCIVMGRLDTLSATIAVMM 369

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIE GLT
Sbjct: 370 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEDGLT 429

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGI+IIACTLSLGTINFPQWGGMF GPKPNVTEEDYY++EWDA EQE GLH+VSMKFAE
Sbjct: 430 YMGILIIACTLSLGTINFPQWGGMFFGPKPNVTEEDYYIKEWDAAEQELGLHKVSMKFAE 489

Query: 301 GSRNERSSKVISVTEKEDSTPT 323
           GSRNERSSK   V+E++ +  T
Sbjct: 490 GSRNERSSKGKYVSEEDGTRST 511

[Close]

BLAST of Spo25105.1 vs. NCBI nr
Match: gi|731367824|ref|XP_010695819.1| (PREDICTED: high affinity nitrate transporter 2.5-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 491.1 bits (1263), Expect = 1.500e-135
Identity = 242/320 (75.62%), Postives = 274/320 (85.62%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y+ I  +    T F+AWR+AFFIP   QMFSA+AVMLFGQDLPDGNF Q+QKSG M
Sbjct: 182 MPLVYNAI--RKSGSTNFSAWRIAFFIPGLFQMFSAFAVMLFGQDLPDGNFHQLQKSGEM 241

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKD ASK++YH VTNYRSWITA+GYGFCFGVELAV N+V YYFYDRFNLNLNLAGIVGA 
Sbjct: 242 HKDNASKVLYHGVTNYRSWITALGYGFCFGVELAVNNIVAYYFYDRFNLNLNLAGIVGAT 301

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FG+VNIFSR  GG  SDFMAKK+GMRGRLWGWW VQTLSGVMCI MG ++TLS+TI VM+
Sbjct: 302 FGLVNIFSRPIGGGLSDFMAKKYGMRGRLWGWWIVQTLSGVMCIAMGLLDTLSATIVVMI 361

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFS  VQAAEGLTFGVVPFVSRRS+GVVSG++GAGGN GAVI QVIFFRGSQF  E G+T
Sbjct: 362 IFSIFVQAAEGLTFGVVPFVSRRSMGVVSGMTGAGGNVGAVITQVIFFRGSQFTTERGIT 421

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMG++IIACTL + +I FPQWGGMF GPK NVTEEDYY+REWD++EQE GLHR+SMKFAE
Sbjct: 422 YMGVMIIACTLGITSIYFPQWGGMFSGPKANVTEEDYYLREWDSKEQELGLHRMSMKFAE 481

Query: 301 GSRNERSSKVISVTEKEDST 321
           GSRNERS+K  S  E++  T
Sbjct: 482 GSRNERSTKAKSDPEEDSKT 499

[Close]

BLAST of Spo25105.1 vs. NCBI nr
Match: gi|731367917|ref|XP_010695865.1| (PREDICTED: high affinity nitrate transporter 2.5-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 486.5 bits (1251), Expect = 3.700e-134
Identity = 239/317 (75.39%), Postives = 274/317 (86.44%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +YS +L KT A TPF AWR+AFF PA LQM SA+AVM+FGQDLPDGNF ++QKSG M
Sbjct: 181 MPLVYSIVL-KTGA-TPFAAWRIAFFFPALLQMSSAFAVMIFGQDLPDGNFQELQKSGDM 240

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKD ASKI+YH +TNYRSWITA+GYGFCFGVELAV N+V YYFYDRFNLNLN++GI+ A 
Sbjct: 241 HKDNASKILYHGITNYRSWITALGYGFCFGVELAVNNIVAYYFYDRFNLNLNISGIIAAS 300

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVN+FSR  GG  SDFMAKKFGMRGRLW WW VQTLSGV+CI +G ++TLS++IAVM+
Sbjct: 301 FGMVNLFSRPIGGGLSDFMAKKFGMRGRLWSWWIVQTLSGVLCIGLGLIDTLSASIAVMI 360

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFS  VQAAEGLTFGVVPFVSRRS+GVVSG++GAGGN GAVI QVIFFRGSQF  E G+T
Sbjct: 361 IFSVFVQAAEGLTFGVVPFVSRRSMGVVSGMTGAGGNVGAVITQVIFFRGSQFATEKGIT 420

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMG +IIACTL + +I FPQWGGMFCGPKP+ TEE+YY+REWDA+EQEQGLH +SMKFAE
Sbjct: 421 YMGFMIIACTLGITSIYFPQWGGMFCGPKPHFTEEEYYLREWDAKEQEQGLHEISMKFAE 480

Query: 301 GSRNERSSKVISVTEKE 318
           GSRNERS K +SV   E
Sbjct: 481 GSRNERSRKAMSVPTVE 495

[Close]

BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q7Q1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_216030 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 1.600e-184
Identity = 326/327 (99.69%), Postives = 327/327 (100.00%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM
Sbjct: 157 MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 216

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL
Sbjct: 217 HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 276

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM
Sbjct: 277 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 336

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT
Sbjct: 337 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 396

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE
Sbjct: 397 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 456

Query: 301 GSRNERSSKVISVTEKEDSTPTPVNNV 328
           GSRNERSSKVISVTE+EDSTPTPVNNV
Sbjct: 457 GSRNERSSKVISVTEEEDSTPTPVNNV 483

[Close]

BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
Match: A1YYP6_SPIOL (High affinity nitrate transporter OS=Spinacia oleracea GN=NRT2 PE=2 SV=3)

HSP 1 Score: 651.4 bits (1679), Expect = 6.000e-184
Identity = 325/327 (99.39%), Postives = 326/327 (99.69%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM
Sbjct: 182 MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 241

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL
Sbjct: 242 HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 301

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSST AVMM
Sbjct: 302 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTTAVMM 361

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT
Sbjct: 362 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 421

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE
Sbjct: 422 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 481

Query: 301 GSRNERSSKVISVTEKEDSTPTPVNNV 328
           GSRNERSSKVISVTE+EDSTPTPVNNV
Sbjct: 482 GSRNERSSKVISVTEEEDSTPTPVNNV 508

[Close]

BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RFD7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_078330 PE=4 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 8.400e-162
Identity = 287/322 (89.13%), Postives = 305/322 (94.72%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP LYS ILNKTPA TPFTAWRVAFFIPA LQMFSA+AVMLFGQDLPDGNFLQMQKSG M
Sbjct: 190 MPLLYSLILNKTPASTPFTAWRVAFFIPAFLQMFSAFAVMLFGQDLPDGNFLQMQKSGEM 249

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDKASK+ YHAVTNYRSWITAIGYGFCFGVELAVTN++V+YFYDRFNLNLNLAGIVGAL
Sbjct: 250 HKDKASKVFYHAVTNYRSWITAIGYGFCFGVELAVTNIIVFYFYDRFNLNLNLAGIVGAL 309

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVNIFSRSSGGWFSDFMAKK+G+RGRLWGWW VQTLSGVMCIVMGR++TLS+TIAVMM
Sbjct: 310 FGMVNIFSRSSGGWFSDFMAKKYGIRGRLWGWWGVQTLSGVMCIVMGRLDTLSATIAVMM 369

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIE GLT
Sbjct: 370 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEDGLT 429

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGI+IIACTLSLGTINFPQWGGMF GPKPNVTEEDYY++EWDA EQE GLH+VSMKFAE
Sbjct: 430 YMGILIIACTLSLGTINFPQWGGMFFGPKPNVTEEDYYIKEWDAAEQELGLHKVSMKFAE 489

Query: 301 GSRNERSSKVISVTEKEDSTPT 323
           GSRNERSSK   V+E++ +  T
Sbjct: 490 GSRNERSSKGKYVSEEDGTRST 511

[Close]

BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BBK4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g155830 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.600e-134
Identity = 239/317 (75.39%), Postives = 274/317 (86.44%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +YS +L KT A TPF AWR+AFF PA LQM SA+AVM+FGQDLPDGNF ++QKSG M
Sbjct: 181 MPLVYSIVL-KTGA-TPFAAWRIAFFFPALLQMSSAFAVMIFGQDLPDGNFQELQKSGDM 240

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKD ASKI+YH +TNYRSWITA+GYGFCFGVELAV N+V YYFYDRFNLNLN++GI+ A 
Sbjct: 241 HKDNASKILYHGITNYRSWITALGYGFCFGVELAVNNIVAYYFYDRFNLNLNISGIIAAS 300

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGMVN+FSR  GG  SDFMAKKFGMRGRLW WW VQTLSGV+CI +G ++TLS++IAVM+
Sbjct: 301 FGMVNLFSRPIGGGLSDFMAKKFGMRGRLWSWWIVQTLSGVLCIGLGLIDTLSASIAVMI 360

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           IFS  VQAAEGLTFGVVPFVSRRS+GVVSG++GAGGN GAVI QVIFFRGSQF  E G+T
Sbjct: 361 IFSVFVQAAEGLTFGVVPFVSRRSMGVVSGMTGAGGNVGAVITQVIFFRGSQFATEKGIT 420

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMG +IIACTL + +I FPQWGGMFCGPKP+ TEE+YY+REWDA+EQEQGLH +SMKFAE
Sbjct: 421 YMGFMIIACTLGITSIYFPQWGGMFCGPKPHFTEEEYYLREWDAKEQEQGLHEISMKFAE 480

Query: 301 GSRNERSSKVISVTEKE 318
           GSRNERS K +SV   E
Sbjct: 481 GSRNERSRKAMSVPTVE 495

[Close]

BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RQX8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044530 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.500e-113
Identity = 192/309 (62.14%), Postives = 248/309 (80.26%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y+ IL    A+  FTAWR+AFFIPA LQ   A+AV++FGQD+PDGNF Q+ KSG  
Sbjct: 183 MPLVYTIILRMGSAN--FTAWRIAFFIPAVLQTVLAFAVLIFGQDMPDGNFQQLHKSGTK 242

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKDK SK+VYH +TNYR WITA+ YG+CFGVEL V N++  YF+DRFNLNL+ AG++ A 
Sbjct: 243 HKDKFSKVVYHGLTNYRGWITALCYGYCFGVELTVDNIIAEYFFDRFNLNLHTAGLIAAC 302

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FG+ N F+R  GG  SD +AKKFGMRGRLWGWW +QT+ G++CI++G MN+LS +IAVM+
Sbjct: 303 FGLANFFARPGGGLVSDLLAKKFGMRGRLWGWWIIQTVGGLLCILLGLMNSLSGSIAVML 362

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS  VQAA GL FGVVPFVSRR+LGV+SG++GAGGN GAV+ Q+IFF+GSQ+  E G+T
Sbjct: 363 VFSVFVQAACGLAFGVVPFVSRRALGVISGMTGAGGNVGAVLTQLIFFKGSQYTTERGIT 422

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMG +I+ACTL +  I FPQWGGMFCGPK   +E+DYY  EWD++E+++G H+ S++FAE
Sbjct: 423 YMGAMIVACTLPISAIYFPQWGGMFCGPKSEFSEDDYYKEEWDSKEKQEGHHQASLRFAE 482

Query: 301 GSRNERSSK 310
            S++ERS K
Sbjct: 483 SSKSERSKK 489

[Close]

BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
Match: NRT25_ARATH (High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana GN=NRT2.5 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 4.100e-101
Identity = 179/307 (58.31%), Postives = 230/307 (74.92%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP ++S I N     T FTAWR+AFFIP   Q  SA+AV+LFGQDLPDG++  M KSG  
Sbjct: 188 MPIVFSLIRNM--GATKFTAWRIAFFIPGLFQTLSAFAVLLFGQDLPDGDYWAMHKSGER 247

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KD   K++ + + NYR WITA+ YG+CFGVEL + N++  YF+DRF+L L  AGI+ A 
Sbjct: 248 EKDDVGKVISNGIKNYRGWITALAYGYCFGVELTIDNIIAEYFFDRFHLKLQTAGIIAAS 307

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FG+ N F+R  GG FSDFM+++FGMRGRLW WW VQT  GV+C  +G++++L+ +I VM+
Sbjct: 308 FGLANFFARPGGGIFSDFMSRRFGMRGRLWAWWIVQTSGGVLCACLGQISSLTVSIIVML 367

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS  VQAA GLTFGVVPF+SRRSLGVVSG++GAGGN GAV+ Q+IFF+GS +  E G+T
Sbjct: 368 VFSVFVQAACGLTFGVVPFISRRSLGVVSGMTGAGGNVGAVLTQLIFFKGSTYTRETGIT 427

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGP-KPNVTEEDYYMREWDAEEQEQGLHRVSMKFA 300
            MG++ IAC+L +  I FPQWGGMFCGP    VTEEDYY+ EW+ EE+E+ LH  S KFA
Sbjct: 428 LMGVMSIACSLPICLIYFPQWGGMFCGPSSKKVTEEDYYLAEWNDEEKEKNLHIGSQKFA 487

Query: 301 EGSRNER 307
           E S +ER
Sbjct: 488 ETSISER 492

[Close]

BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
Match: NRT23_ORYSJ (High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica GN=NRT2.3 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 3.600e-97
Identity = 171/306 (55.88%), Postives = 228/306 (74.51%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  I       TPFTAWR+AFFIP  +Q FSA AV+ FGQD+P GN+ ++ K+G M
Sbjct: 189 MPLVYEAI--HKIGSTPFTAWRIAFFIPGLMQTFSAIAVLAFGQDMPGGNYGKLHKTGDM 248

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           HKD    ++ HA+TNYR WI A+ YG+ FGVEL + NVV  YFYDRF++NL  AG++ A 
Sbjct: 249 HKDSFGNVLRHALTNYRGWILALTYGYSFGVELTIDNVVHQYFYDRFDVNLQTAGLIAAS 308

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMN-TLSSTIAVM 180
           FGM NI SR  GG  SD+++ ++GMRGRLWG WTVQT+ GV+C+V+G ++ + ++++AVM
Sbjct: 309 FGMANIISRPGGGLLSDWLSSRYGMRGRLWGLWTVQTIGGVLCVVLGIVDFSFAASVAVM 368

Query: 181 MIFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGL 240
           ++FSF VQAA GLTFG+VPFVSRRSLG++SG++G GGN GAV+ Q IFF G+++K E G+
Sbjct: 369 VLFSFFVQAACGLTFGIVPFVSRRSLGLISGMTGGGGNVGAVLTQYIFFHGTKYKTETGI 428

Query: 241 TYMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFA 300
            YMG++IIACTL +  I FPQWGGM  GP+   T E+YY REW   E+E+G +  S++FA
Sbjct: 429 KYMGLMIIACTLPVMLIYFPQWGGMLVGPRKGATAEEYYSREWSDHEREKGFNAASVRFA 488

Query: 301 EGSRNE 306
           E S  E
Sbjct: 489 ENSVRE 492

[Close]

BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
Match: NRT21_ORYSJ (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.600e-87
Identity = 161/326 (49.39%), Postives = 222/326 (68.10%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  ++ K  A TPFTAWR+A+F+P  L +     V+  GQDLPDGN   +QK G +
Sbjct: 207 MPLVYD-VIRKCGA-TPFTAWRLAYFVPGTLHVVMGVLVLTLGQDLPDGNLRSLQKKGDV 266

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           ++D  S+++++AVTNYR+WI  + YG+  GVEL   NV+  YFYDRF+L+L +AGI+ A 
Sbjct: 267 NRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEYFYDRFDLDLRVAGIIAAS 326

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM NI +R +GG  SD  A+ FGMR RLW  W +QT  G  C+++GR +TL +++  M+
Sbjct: 327 FGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFCLLLGRASTLPTSVVCMV 386

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FSF  QAA G  FGV+PFVSRRSLG++SG++GAGGN GA + Q++FF  S++    GL 
Sbjct: 387 LFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGLTQLLFFTSSRYSTGTGLE 446

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGI+I+ACTL +  ++FPQWG MF  P     EE YY  EW  +E+ +GLH  S+KFAE
Sbjct: 447 YMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAEEEHYYGSEWSEQEKSKGLHGASLKFAE 506

Query: 301 GSRNERSSKVISVTEKEDSTPTPVNN 327
            SR+ER  +  +V     +  TP NN
Sbjct: 507 NSRSERGRR--NVINAAAAAATPPNN 528

[Close]

BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
Match: NRT22_ORYSJ (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.600e-87
Identity = 161/326 (49.39%), Postives = 222/326 (68.10%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  ++ K  A TPFTAWR+A+F+P  L +     V+  GQDLPDGN   +QK G +
Sbjct: 207 MPLVYD-VIRKCGA-TPFTAWRLAYFVPGTLHVVMGVLVLTLGQDLPDGNLRSLQKKGDV 266

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
           ++D  S+++++AVTNYR+WI  + YG+  GVEL   NV+  YFYDRF+L+L +AGI+ A 
Sbjct: 267 NRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEYFYDRFDLDLRVAGIIAAS 326

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM NI +R +GG  SD  A+ FGMR RLW  W +QT  G  C+++GR +TL +++  M+
Sbjct: 327 FGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFCLLLGRASTLPTSVVCMV 386

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FSF  QAA G  FGV+PFVSRRSLG++SG++GAGGN GA + Q++FF  S++    GL 
Sbjct: 387 LFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGLTQLLFFTSSRYSTGTGLE 446

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTEEDYYMREWDAEEQEQGLHRVSMKFAE 300
           YMGI+I+ACTL +  ++FPQWG MF  P     EE YY  EW  +E+ +GLH  S+KFAE
Sbjct: 447 YMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAEEEHYYGSEWSEQEKSKGLHGASLKFAE 506

Query: 301 GSRNERSSKVISVTEKEDSTPTPVNN 327
            SR+ER  +  +V     +  TP NN
Sbjct: 507 NSRSERGRR--NVINAAAAAATPPNN 528

[Close]

BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
Match: NRT24_ARATH (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 9.800e-87
Identity = 156/315 (49.52%), Postives = 215/315 (68.25%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  I  +    T FTAWR+AFF+P  + +     V+  GQDLPDGN   ++K GA+
Sbjct: 206 MPMVYEII--RRLGSTSFTAWRMAFFVPGWMHIIMGILVLTLGQDLPDGNRSTLEKKGAV 265

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KDK SK++++A+TNYR+W+  + YG+  GVEL   NV+  YF+DRF+L L+ AGI+ A 
Sbjct: 266 TKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEYFFDRFHLKLHTAGIIAAS 325

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM N F+R  GGW SD  A++FGMRGRLW  W +QTL G  C+ +GR  TL + +  M+
Sbjct: 326 FGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFFCLWLGRATTLPTAVVFMI 385

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS   QAA G TF ++PF+SRRSLG++SGL+GAGGN G+ + Q++FF  S F  E GLT
Sbjct: 386 LFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTSTFSTEQGLT 445

Query: 241 YMGIVIIACTLSLGTINFPQWGGMF---CGPKPNVTEEDYYMREWDAEEQEQGLHRVSMK 300
           +MG++I+ACTL +  ++FPQWG MF      +   TEE YYM+EW   E+ +G+H  S+K
Sbjct: 446 WMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEEYYYMKEWTETEKRKGMHEGSLK 505

Query: 301 FAEGSRNERSSKVIS 313
           FA  SR+ER  +V S
Sbjct: 506 FAVNSRSERGRRVAS 518

[Close]

BLAST of Spo25105.1 vs. TAIR (Arabidopsis)
Match: AT1G12940.1 (nitrate transporter2.5)

HSP 1 Score: 369.4 bits (947), Expect = 2.300e-102
Identity = 179/307 (58.31%), Postives = 230/307 (74.92%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP ++S I N     T FTAWR+AFFIP   Q  SA+AV+LFGQDLPDG++  M KSG  
Sbjct: 188 MPIVFSLIRNM--GATKFTAWRIAFFIPGLFQTLSAFAVLLFGQDLPDGDYWAMHKSGER 247

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KD   K++ + + NYR WITA+ YG+CFGVEL + N++  YF+DRF+L L  AGI+ A 
Sbjct: 248 EKDDVGKVISNGIKNYRGWITALAYGYCFGVELTIDNIIAEYFFDRFHLKLQTAGIIAAS 307

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FG+ N F+R  GG FSDFM+++FGMRGRLW WW VQT  GV+C  +G++++L+ +I VM+
Sbjct: 308 FGLANFFARPGGGIFSDFMSRRFGMRGRLWAWWIVQTSGGVLCACLGQISSLTVSIIVML 367

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS  VQAA GLTFGVVPF+SRRSLGVVSG++GAGGN GAV+ Q+IFF+GS +  E G+T
Sbjct: 368 VFSVFVQAACGLTFGVVPFISRRSLGVVSGMTGAGGNVGAVLTQLIFFKGSTYTRETGIT 427

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGP-KPNVTEEDYYMREWDAEEQEQGLHRVSMKFA 300
            MG++ IAC+L +  I FPQWGGMFCGP    VTEEDYY+ EW+ EE+E+ LH  S KFA
Sbjct: 428 LMGVMSIACSLPICLIYFPQWGGMFCGPSSKKVTEEDYYLAEWNDEEKEKNLHIGSQKFA 487

Query: 301 EGSRNER 307
           E S +ER
Sbjct: 488 ETSISER 492

[Close]

BLAST of Spo25105.1 vs. TAIR (Arabidopsis)
Match: AT5G60770.1 (nitrate transporter 2.4)

HSP 1 Score: 321.6 bits (823), Expect = 5.500e-88
Identity = 156/315 (49.52%), Postives = 215/315 (68.25%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  I  +    T FTAWR+AFF+P  + +     V+  GQDLPDGN   ++K GA+
Sbjct: 206 MPMVYEII--RRLGSTSFTAWRMAFFVPGWMHIIMGILVLTLGQDLPDGNRSTLEKKGAV 265

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KDK SK++++A+TNYR+W+  + YG+  GVEL   NV+  YF+DRF+L L+ AGI+ A 
Sbjct: 266 TKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEYFFDRFHLKLHTAGIIAAS 325

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM N F+R  GGW SD  A++FGMRGRLW  W +QTL G  C+ +GR  TL + +  M+
Sbjct: 326 FGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFFCLWLGRATTLPTAVVFMI 385

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS   QAA G TF ++PF+SRRSLG++SGL+GAGGN G+ + Q++FF  S F  E GLT
Sbjct: 386 LFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTSTFSTEQGLT 445

Query: 241 YMGIVIIACTLSLGTINFPQWGGMF---CGPKPNVTEEDYYMREWDAEEQEQGLHRVSMK 300
           +MG++I+ACTL +  ++FPQWG MF      +   TEE YYM+EW   E+ +G+H  S+K
Sbjct: 446 WMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEEYYYMKEWTETEKRKGMHEGSLK 505

Query: 301 FAEGSRNERSSKVIS 313
           FA  SR+ER  +V S
Sbjct: 506 FAVNSRSERGRRVAS 518

[Close]

BLAST of Spo25105.1 vs. TAIR (Arabidopsis)
Match: AT1G08090.1 (nitrate transporter 2:1)

HSP 1 Score: 319.7 bits (818), Expect = 2.100e-87
Identity = 157/327 (48.01%), Postives = 223/327 (68.20%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  I  +    T FTAWR+AFF+P  L +     V+  GQDLPDGN   ++K+G +
Sbjct: 206 MPIVYEII--RRCGSTAFTAWRIAFFVPGWLHIIMGILVLNLGQDLPDGNRATLEKAGEV 265

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KDK  KI+++AVTNYR+WI  + YG+  GVEL+  NV+  YF+DRF+L L+ AG++ A 
Sbjct: 266 AKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGLIAAC 325

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM N F+R +GG+ SDF AK FGMRGRLW  W +QT  G+ C+ +GR NTL + +  M+
Sbjct: 326 FGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTAGGLFCVWLGRANTLVTAVVAMV 385

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS   QAA G TF +VPFVSRR+LG++SGL+GAGGN G+ + Q++FF  S F  E GLT
Sbjct: 386 LFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQLLFFSTSHFTTEQGLT 445

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNV---TEEDYYMREWDAEEQEQGLHRVSMK 300
           +MG++I+ACTL +  ++FPQWG MF  P  +    TE  YY  EW+ +E+++ +H+ S++
Sbjct: 446 WMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEAHYYGSEWNEQEKQKNMHQGSLR 505

Query: 301 FAEGSRNERSSKVISVTEKEDSTPTPV 325
           FAE +++E   +V S     ++TP  V
Sbjct: 506 FAENAKSEGGRRVRSAATPPENTPNNV 530

[Close]

BLAST of Spo25105.1 vs. TAIR (Arabidopsis)
Match: AT1G08100.1 (nitrate transporter 2.2)

HSP 1 Score: 316.6 bits (810), Expect = 1.800e-86
Identity = 157/312 (50.32%), Postives = 214/312 (68.59%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP +Y  I  +    T FTAWR+AFF+P  L +     V+  GQDLP GN   M+K+G +
Sbjct: 204 MPIVYEII--RRCGSTAFTAWRIAFFVPGWLHIIMGILVLTLGQDLPGGNRAAMEKAGEV 263

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KDK  KI+++AVTNYR+WI  + YG+  GVEL+  NV+  YF+DRF+L L+ AGI+ A 
Sbjct: 264 AKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGIIAAC 323

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM N F+R +GGW SD  AK+FGMRGRLW  W +QT  G+ C+ +GR NTL + +  M+
Sbjct: 324 FGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSGGLFCVWLGRANTLVTAVVSMV 383

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS   QAA G TF +VPFVSRR+LG++SGL+GAGGN G+ + Q++FF  S+F  E GLT
Sbjct: 384 LFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQLVFFSTSRFTTEEGLT 443

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPNVTE--EDYYMREWDAEEQEQGLHRVSMKF 300
           +MG++I+ACTL +  I+FPQWG MF  P  +  +  E YY+ E+  EEQ+ G+H  S  F
Sbjct: 444 WMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHYYVGEYSKEEQQIGMHLKSKLF 503

Query: 301 AEGSRNERSSKV 311
           A+G++ E  S V
Sbjct: 504 ADGAKTEGGSSV 513

[Close]

BLAST of Spo25105.1 vs. TAIR (Arabidopsis)
Match: AT3G45060.1 (high affinity nitrate transporter 2.6)

HSP 1 Score: 310.5 bits (794), Expect = 1.300e-84
Identity = 165/328 (50.30%), Postives = 211/328 (64.33%), Query Frame = 1


		  
Query: 1   MPFLYSFILNKTPADTPFTAWRVAFFIPAGLQMFSAYAVMLFGQDLPDGNFLQMQKSGAM 60
           MP ++  I  K    TPFTAWR AFFIP  LQ+     V+  GQDLPDGN   +QKSG +
Sbjct: 216 MPMVFHVI--KLTGATPFTAWRFAFFIPGILQIVMGILVLTLGQDLPDGNLSTLQKSGQV 275

Query: 61  HKDKASKIVYHAVTNYRSWITAIGYGFCFGVELAVTNVVVYYFYDRFNLNLNLAGIVGAL 120
            KDK SK+ + AV NYR+WI  + YGF  GVEL + NV+  YFYDRFNL L+ AGI+ A 
Sbjct: 276 SKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTINNVISGYFYDRFNLTLHTAGIIAAS 335

Query: 121 FGMVNIFSRSSGGWFSDFMAKKFGMRGRLWGWWTVQTLSGVMCIVMGRMNTLSSTIAVMM 180
           FGM N F+R  GG+ SD  A+ FGMRGRLW  W +QT+  + CI +GR ++L   I  MM
Sbjct: 336 FGMANFFARPFGGYASDVAARLFGMRGRLWILWILQTVGALFCIWLGRASSLPIAILAMM 395

Query: 181 IFSFLVQAAEGLTFGVVPFVSRRSLGVVSGLSGAGGNAGAVICQVIFFRGSQFKIEHGLT 240
           +FS   QAA G  FGV PFVSRRSLG++SGL+GAGGN G+ + Q++FF  S+F    GL+
Sbjct: 396 LFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNFGSGVTQLLFFSSSRFSTAEGLS 455

Query: 241 YMGIVIIACTLSLGTINFPQWGGMFCGPKPN---VTEEDYYMREWDAEEQEQGLHRVSMK 300
            MG++ + C+L +  I+FPQWG MF  P  +     EE YY  EW  EE+  GLH  S+K
Sbjct: 456 LMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKSKEEHYYGAEWTEEEKSLGLHEGSIK 515

Query: 301 FAEGSRNERSSKVISVTEKEDSTPTPVN 326
           FAE SR+ER  K +        T +P +
Sbjct: 516 FAENSRSERGRKAMLADIPTPETGSPAH 541

The following BLAST results are available for this feature:

BLAST of Spo25105.1 vs. NCBI nr
BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
BLAST of Spo25105.1 vs. TAIR (Arabidopsis)

BLAST of Spo25105.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902038373\|gb\|KNA02718.1\|	2.3e-184	99.6	hypothetical protein SOVF_2160...	[more]
gi\|166209891\|gb\|ABL89187.3\|	8.6e-184	99.3	high affinity nitrate transpor...	[more]
gi\|902216966\|gb\|KNA17572.1\|	1.2e-161	89.1	hypothetical protein SOVF_0783...	[more]
gi\|731367824\|ref\|XP_010695819.1\|	1.5e-135	75.6	PREDICTED: high affinity nitra...	[more]
gi\|731367917\|ref\|XP_010695865.1\|	3.7e-134	75.3	PREDICTED: high affinity nitra...	[more]

BLAST of Spo25105.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q7Q1_SPIOL	1.6e-184	99.6	Uncharacterized protein OS=Spi...	[more]
A1YYP6_SPIOL	6.0e-184	99.3	High affinity nitrate transpor...	[more]
A0A0K9RFD7_SPIOL	8.4e-162	89.1	Uncharacterized protein OS=Spi...	[more]
A0A0J8BBK4_BETVU	2.6e-134	75.3	Uncharacterized protein OS=Bet...	[more]
A0A0K9RQX8_SPIOL	2.5e-113	62.1	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo25105.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
NRT25_ARATH	4.1e-101	58.3	High affinity nitrate transpor...	[more]
NRT23_ORYSJ	3.6e-97	55.8	High-affinity nitrate transpor...	[more]
NRT21_ORYSJ	2.6e-87	49.3	High-affinity nitrate transpor...	[more]
NRT22_ORYSJ	2.6e-87	49.3	High-affinity nitrate transpor...	[more]
NRT24_ARATH	9.8e-87	49.5	High affinity nitrate transpor...	[more]

BLAST of Spo25105.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G12940.1	2.3e-102	58.3	nitrate transporter2.5	[more]
AT5G60770.1	5.5e-88	49.5	nitrate transporter 2.4	[more]
AT1G08090.1	2.1e-87	48.0	nitrate transporter 2:1	[more]
AT1G08100.1	1.8e-86	50.3	nitrate transporter 2.2	[more]
AT3G45060.1	1.3e-84	50.3	high affinity nitrate transpor...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020846	Major facilitator superfamily domain	unknown	SSF103473	MFS general substrate transporter	coord: 6..253 score: 1.14
None	No IPR available	GENE3D	1.20.1250.20		coord: 72..251 score: 4.3
None	No IPR available	PANTHER	PTHR23515	FAMILY NOT NAMED	coord: 1..324 score: 1.5E
None	No IPR available	PANTHER	PTHR23515:SF3	HIGH AFFINITY NITRATE TRANSPORTER 2.5	coord: 1..324 score: 1.5E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane