Spo25287.1 (mRNA) | SpinachBase

Overview


Name	Spo25287.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Actin depolymerizing factor) (Actin depolymerizing factor 8)
Location	SpoScf_02273 : 8605 .. 11573 (+)
Sequence length	812

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTCTTGGTTCCAACAAATATATACCCCACAGAAAAAATACACAAATTTTATTTTCTTTAAAATAAAACAAAAGAATAAAAAAACAGTCTCCATTTTATGCACCCCTTATTTCTCATTCTCTCTCTATCTGCGTCTTTCTCTCTCCATCAAAGCTCTAGATTCTCTCCATTTTCTCTCTCTCCTACTCCCACAGGTGCTGCTACCACTTCACTGAGAACAAGAACAAAAAATGGTATAATACTTTTTTAAAAGTTTTTGTTGATTTTTAGTTTTGGATTCTAGGGTTAGTTCTCTTGATTATGATTTGTGTTTGATTTTTTTTTGGAATTATAGTCGGATTTTTTTTTTGTCTTTTGCTTTCGATCTGGAAATTTTGTGAACAGAACGATAGATTTAGTTTGAAAATTTTGGATATGCGTGGATTTGAGCGTGTTTTCGGAGATGTTTCGTGAACAATTATGCTTTTTTCTGATCTGAAAGTTATTTATTCGTTTTGTTTTTAATTTTTTTGAACTTTTCTGTTGTTACAATATCGAGCTCTGGAATCAATAGCATCTTCTGCGCTGGGATTTTAGAAACTTTAGTTATTTTTGGTACTTATTACGAATTTTATGAATTTGTTTCTGGCTTGCATGATTTTGGAATTAGTGGAACACAACTGAGGATCTGTAATTTGGCTGAGGAATCAGGTTGGTTAGAAATGGATAGCTGCAGTAGCTCGAATTCAGTGTTTTATTCTAAATAAGCATGAGCGAATAGTATAATTTGTTTGATTGGATGTGGTGAGTTTGATTAGACAAAAAATTGTGATGAATTTGATAACACTAATTTGTTTCGTGCTATTGAGTATATGGTTTTCGGCCAGATGCAATGATGATTGGTAATGTGTCTTGTGTTTTGATGTGTGGTTGAATGTCTTGAAGAAGATTGTGTGATTCGTGCTAAGGAAATTCATCGCATAGAATTAGCAAATCCTTATTTTGTGGATATTATCATCTTGAGGATCCTATTTTAGAATGTTGTTGATGGTAGAGAACAATGTTACCTGGTTCGCTAGTATGAGATTGGGTACGGGTTCGCAATTCGCTAGCTAATTTGCTAAATTAGACCAAAATTATACCATTTTGATGTTGTAATTTGCTTTTCCGGTTCGCGGTTCGGGGGCTGATTGGAGGATTAGGTAACACCGGTAGAGAATGAACCTAAGGGTAGACACTTCCCGAGGAAATCTTGTGTTGTGTTGGATTTTGTTAAACGATTGTTAGAATTGTCTGTTCAATTTATTTTTTTGACGCGCTGGAGAGTGTGTTCATGGTTTTCTTGGTGTATAAGTTAGGCTACTGATGACCTCTCTTAAGGTTTTGAACTTCTTTTTGTTCGTTGTAAATTTACAGTATGTTTTACAATCGAGGCCCAAAATCTAGAGGGAAAAGGAGCATTTTACTATGTGCTGTCTCCATTTCTCATTGGAGTCTCGGTTTTCATCTGTTTTGCTACCAAAAGACTTCAGAAATCAGTTGTAAAAAGAACCTCAGTTCATTGAGCGTTGACACTGGGTTATAATTCGTCAAGAGTTTAATTGTTTTTTGGTGCCATAGAGCCATTTTCTTTATGCATATAGTTTCTTGTTTATTTGAGATGTTGGGTTTCCCTAATTAAGTGGAGTAAGTGTTCTGGATAACCATGAACTTCATTGGGAACTTCATTGGAAGGATTGTATTTGTGACCTGTTGAGTGAAAGAAGATGGAGAGAGCTTTAGTTTACCAAAAAGTTACTGAGTGCTGTGATGTGAAAACTTTAACCAGTACCTAGTATGCTTGGTAATAGAAGAGCTTGTAATTGTATTATAGTAATCCACGGGTATGAAGATGCAGGATTGAAATTGATTTCTCCTTTTATTGCGCTTCTTTTTAATTCGAAGTGCAGCTAAAAGCTTTTAACTTATTTTTACCAAAAAAAATCTCATGTGATGTTTCACTTAATTACTCCAGTACAGCATGTGCTTTTTTCTAATATTTCTATTGAGACCCATCCAAAAACCATGCTTCCAAATTCAGTGGTCTCACCTTGGTTCCTCAAAAAAATGTCTTTGAATATTACCCCCTTCTGTTTTATGCCAATTGTAACTTGTGAGATTATAGCAGCTCCTTGTTGATGCCACAAATATAGCTTAAAGGAAAATTAGGCTTTGGAATGACTTTTCTATGAAACTTTTGACATAGTAGTTAGTAGTATATCTTGTCATATGATTGTGACATATGTTGACATTATTGTATAGGCCAACGCTGCATCAGGAATGGCTGTTGATGATCAGTGCAAGCTCAAATTTTTAGAGCTGAAGGCGAAAAGGACATACCGCTTCATTGTATTTAAGATTGAAGAAAAGCAAAAGCAGGTTATTGTGGAAAAGGTTGGTGAGCCTAACGAAAGCTACGAGGATTTCACTAAGAACATTCCTGCTGATGAGTGCAGATATGCTGTGTATGATTTTGATTATGTGACTGCTGAGAATTGCCAAAAGAGCAGGATCTTTTTCATTGCATGGTAATTTCTTATGAACCAAAACAAAAGCATTCACTCAGTAAAGTGTTATGTTTCTGTGATTGATTAGATTATATTATATTAACCCCTTGAATTGCTTTGAAAGGTCCCCTGACAATGCAAAGGTGAGAAGCAAGATGATATACGCAAGCTCAAAGGACAGGTTCAAGAGAGAGCTGGATGGTATTCAAGTTGAATTGCAAGCGACCGATCCTTCTGAGATGGATTTGGATGTTATTAAAAGCCGTGTCGTGTAATGCTTCACGCGATGCTTCACTGCCTTGATTGTGCGTTATCTATACAGTCCACTTTACCTATGTTCAAATGTTAATACCTTCCTAAGGGGCAGTAGAATTTGCTTACATATAATATTTGATGGTTGTTGCAGTGTTATACTGATGTCTTTTTTTCTTCTGGTTCGGTAA

mRNA sequence

AACTCTTGGTTCCAACAAATATATACCCCACAGAAAAAATACACAAATTTTATTTTCTTTAAAATAAAACAAAAGAATAAAAAAACAGTCTCCATTTTATGCACCCCTTATTTCTCATTCTCTCTCTATCTGCGTCTTTCTCTCTCCATCAAAGCTCTAGATTCTCTCCATTTTCTCTCTCTCCTACTCCCACAGGTGCTGCTACCACTTCACTGAGAACAAGAACAAAAAATGTACCTAGTATGCTTGGTAATAGAAGAGCTTGTAATTGTATTATAGCCAACGCTGCATCAGGAATGGCTGTTGATGATCAGTGCAAGCTCAAATTTTTAGAGCTGAAGGCGAAAAGGACATACCGCTTCATTGTATTTAAGATTGAAGAAAAGCAAAAGCAGGTTATTGTGGAAAAGGTTGGTGAGCCTAACGAAAGCTACGAGGATTTCACTAAGAACATTCCTGCTGATGAGTGCAGATATGCTGTGTATGATTTTGATTATGTGACTGCTGAGAATTGCCAAAAGAGCAGGATCTTTTTCATTGCATGGTCCCCTGACAATGCAAAGGTGAGAAGCAAGATGATATACGCAAGCTCAAAGGACAGGTTCAAGAGAGAGCTGGATGGTATTCAAGTTGAATTGCAAGCGACCGATCCTTCTGAGATGGATTTGGATGTTATTAAAAGCCGTGTCGTTCCACTTTACCTATGTTCAAATGTTAATACCTTCCTAAGGGGCAGTAGAATTTGCTTACATATAATATTTGATGGTTGTTGCAGTGTTATACTGATGTCTTTTTTTCTTCTGGTTCGGTAA

Coding sequence (CDS)

ATGCACCCCTTATTTCTCATTCTCTCTCTATCTGCGTCTTTCTCTCTCCATCAAAGCTCTAGATTCTCTCCATTTTCTCTCTCTCCTACTCCCACAGGTGCTGCTACCACTTCACTGAGAACAAGAACAAAAAATGTACCTAGTATGCTTGGTAATAGAAGAGCTTGTAATTGTATTATAGCCAACGCTGCATCAGGAATGGCTGTTGATGATCAGTGCAAGCTCAAATTTTTAGAGCTGAAGGCGAAAAGGACATACCGCTTCATTGTATTTAAGATTGAAGAAAAGCAAAAGCAGGTTATTGTGGAAAAGGTTGGTGAGCCTAACGAAAGCTACGAGGATTTCACTAAGAACATTCCTGCTGATGAGTGCAGATATGCTGTGTATGATTTTGATTATGTGACTGCTGAGAATTGCCAAAAGAGCAGGATCTTTTTCATTGCATGGTCCCCTGACAATGCAAAGGTGAGAAGCAAGATGATATACGCAAGCTCAAAGGACAGGTTCAAGAGAGAGCTGGATGGTATTCAAGTTGAATTGCAAGCGACCGATCCTTCTGAGATGGATTTGGATGTTATTAAAAGCCGTGTCGTTCCACTTTACCTATGTTCAAATGTTAATACCTTCCTAAGGGGCAGTAGAATTTGCTTACATATAATATTTGATGGTTGTTGCAGTGTTATACTGATGTCTTTTTTTCTTCTGGTTCGGTAA

Protein sequence

MHPLFLILSLSASFSLHQSSRFSPFSLSPTPTGAATTSLRTRTKNVPSMLGNRRACNCIIANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNIPADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVELQATDPSEMDLDVIKSRVVPLYLCSNVNTFLRGSRICLHIIFDGCCSVILMSFFLLVR

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo25287	Spo25287	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo25287.1	Spo25287.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25287.1.exon.1	Spo25287.1.exon.1	exon
Spo25287.1.exon.2	Spo25287.1.exon.2	exon
Spo25287.1.exon.3	Spo25287.1.exon.3	exon
Spo25287.1.exon.4	Spo25287.1.exon.4	exon
Spo25287.1.exon.5	Spo25287.1.exon.5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25287.1.utr5p.1	Spo25287.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25287.1.CDS.1	Spo25287.1.CDS.1	CDS
Spo25287.1.CDS.2	Spo25287.1.CDS.2	CDS
Spo25287.1.CDS.3	Spo25287.1.CDS.3	CDS
Spo25287.1.CDS.4	Spo25287.1.CDS.4	CDS
Spo25287.1.CDS.5	Spo25287.1.CDS.5	CDS

Homology

[Close]

BLAST of Spo25287.1 vs. NCBI nr
Match: gi|902111342|gb|KNA03751.1| (hypothetical protein SOVF_206070 [Spinacia oleracea])

HSP 1 Score: 274.2 bits (700), Expect = 2.100e-70
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRVV 199
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25287.1 vs. NCBI nr
Match: gi|902197140|gb|KNA13337.1| (hypothetical protein SOVF_117910 [Spinacia oleracea])

HSP 1 Score: 273.9 bits (699), Expect = 2.700e-70
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQV+VEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRVV 199
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25287.1 vs. NCBI nr
Match: gi|731318670|ref|XP_010669850.1| (PREDICTED: actin-depolymerizing factor 4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 257.7 bits (657), Expect = 2.000e-65
Identity = 128/137 (93.43%), Postives = 133/137 (97.08%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQVIVEKVGEP+ESYEDF  +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFAASI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD+VTAENCQKSRIFFIAWSPD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWSPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25287.1 vs. NCBI nr
Match: gi|731318668|ref|XP_010669849.1| (PREDICTED: actin-depolymerizing factor 4-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 257.3 bits (656), Expect = 2.600e-65
Identity = 127/137 (92.70%), Postives = 134/137 (97.81%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQVIVEKVGEP+ESYEDFT +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFTASI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD+VTAENCQKSRIFFIAW+PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWAPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDP+EMDLDVIKSR
Sbjct: 121 LQATDPTEMDLDVIKSR 137

[Close]

BLAST of Spo25287.1 vs. NCBI nr
Match: gi|763811997|gb|KJB78849.1| (hypothetical protein B456_013G022900 [Gossypium raimondii])

HSP 1 Score: 247.7 bits (631), Expect = 2.100e-62
Identity = 123/137 (89.78%), Postives = 130/137 (94.89%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKLKFLELKAKRTYRFIVFKIEEKQKQV+VEKVGEP +SYE FT ++
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTDSYEAFTASL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD+VT ENCQKSRIFFIAWSPD +KVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTDENCQKSRIFFIAWSPDTSKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QB58_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_206070 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.400e-70
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRVV 199
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R3F8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_117910 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.900e-70
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQV+VEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRVV 199
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D098_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g037400 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.400e-65
Identity = 128/137 (93.43%), Postives = 133/137 (97.08%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQVIVEKVGEP+ESYEDF  +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFAASI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD+VTAENCQKSRIFFIAWSPD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWSPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CVB6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g037390 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.800e-65
Identity = 127/137 (92.70%), Postives = 134/137 (97.81%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQVIVEKVGEP+ESYEDFT +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFTASI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD+VTAENCQKSRIFFIAW+PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWAPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDP+EMDLDVIKSR
Sbjct: 121 LQATDPTEMDLDVIKSR 137

[Close]

BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
Match: A0A0D2V9F2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G022900 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.400e-62
Identity = 123/137 (89.78%), Postives = 130/137 (94.89%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKLKFLELKAKRTYRFIVFKIEEKQKQV+VEKVGEP +SYE FT ++
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTDSYEAFTASL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD+VT ENCQKSRIFFIAWSPD +KVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTDENCQKSRIFFIAWSPDTSKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
Match: ADF4_ARATH (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana GN=ADF4 PE=1 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 4.600e-62
Identity = 118/138 (85.51%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQVIVEKVGEP  +YEDF  ++
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRV 198
           LQATDP+EMDLDV+KSRV
Sbjct: 121 LQATDPTEMDLDVLKSRV 138

[Close]

BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
Match: ADF2_PETHY (Actin-depolymerizing factor 2 OS=Petunia hybrida GN=ADF2 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 5.100e-61
Identity = 115/137 (83.94%), Postives = 127/137 (92.70%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKLKFLELKAKRTYRFI++KIEEKQK+V+VEK+GEP ESYEDFT  +
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYAVYDFD++T EN QKSRIFFIAWSPD A+VRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDP+EM LDV +SR
Sbjct: 121 LQATDPTEMGLDVFRSR 137

[Close]

BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
Match: ADF1_PETHY (Actin-depolymerizing factor 1 OS=Petunia hybrida GN=ADF1 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.500e-60
Identity = 114/137 (83.21%), Postives = 127/137 (92.70%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQV+VEK+GEP ESYEDF  ++
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           P +ECRYAVYDFD+VTAENCQKSRIFFIAW PD A+VRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQA DP+EM LDVI+SR
Sbjct: 121 LQACDPTEMGLDVIQSR 137

[Close]

BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
Match: ADF1_ARATH (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana GN=ADF1 PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 3.300e-60
Identity = 113/137 (82.48%), Postives = 128/137 (93.43%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVG+P ++YE+F   +
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDP+EMDLDV +SR
Sbjct: 121 LQATDPTEMDLDVFRSR 137

[Close]

BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
Match: ADF10_ARATH (Actin-depolymerizing factor 10 OS=Arabidopsis thaliana GN=ADF10 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.100e-58
Identity = 107/138 (77.54%), Postives = 126/138 (91.30%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +AN+ASGM V D+CKLKFLELKAKR YRFIVFKI+EK +QV+++K+G P E+YEDFT++I
Sbjct: 1   MANSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           P DECRYAVYD+D+ T ENCQKS+IFFIAWSPD ++VRSKM+YASSKDRFKRELDGIQVE
Sbjct: 61  PEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRV 198
           LQATDPSEM LD+IK RV
Sbjct: 121 LQATDPSEMSLDIIKGRV 138

[Close]

BLAST of Spo25287.1 vs. TAIR (Arabidopsis)
Match: AT5G59890.1 (actin depolymerizing factor 4)

HSP 1 Score: 239.2 bits (609), Expect = 2.600e-63
Identity = 118/138 (85.51%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQVIVEKVGEP  +YEDF  ++
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRV 198
           LQATDP+EMDLDV+KSRV
Sbjct: 121 LQATDPTEMDLDVLKSRV 138

[Close]

BLAST of Spo25287.1 vs. TAIR (Arabidopsis)
Match: AT3G46010.2 (actin depolymerizing factor 1)

HSP 1 Score: 232.6 bits (592), Expect = 2.400e-61
Identity = 113/136 (83.09%), Postives = 127/136 (93.38%), Query Frame = 1


		  
Query: 61  ANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNIP 120
           ANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVG+P ++YE+F   +P
Sbjct: 13  ANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP 72

Query: 121 ADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVEL 180
           ADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVEL
Sbjct: 73  ADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVEL 132

Query: 181 QATDPSEMDLDVIKSR 197
           QATDP+EMDLDV +SR
Sbjct: 133 QATDPTEMDLDVFRSR 148

[Close]

BLAST of Spo25287.1 vs. TAIR (Arabidopsis)
Match: AT1G01750.1 (actin depolymerizing factor 11)

HSP 1 Score: 228.0 bits (580), Expect = 6.000e-60
Identity = 107/138 (77.54%), Postives = 126/138 (91.30%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +AN+ASGM V D+CKLKFLELKAKR YRFIVFKI+EK +QV+++K+G P E+YEDFT++I
Sbjct: 1   MANSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           P DECRYAVYD+D+ T ENCQKS+IFFIAWSPD ++VRSKM+YASSKDRFKRELDGIQVE
Sbjct: 61  PEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRV 198
           LQATDPSEM LD+IK RV
Sbjct: 121 LQATDPSEMSLDIIKGRV 138

[Close]

BLAST of Spo25287.1 vs. TAIR (Arabidopsis)
Match: AT4G00680.1 (actin depolymerizing factor 8)

HSP 1 Score: 223.4 bits (568), Expect = 1.500e-58
Identity = 104/138 (75.36%), Postives = 126/138 (91.30%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +AN+ASGM V+D+CK+KFLELKAKRTYRFIVFKI+EK +QV +EK+G P E+Y+DFT +I
Sbjct: 1   MANSASGMHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSI 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           P DECRYAVYDFD+ T +NCQKS+IFFIAWSPD ++VRSKM+YASSKDRFKRE++GIQVE
Sbjct: 61  PDDECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVE 120

Query: 180 LQATDPSEMDLDVIKSRV 198
           LQATDPSEM LD+IK R+
Sbjct: 121 LQATDPSEMSLDIIKGRL 138

[Close]

BLAST of Spo25287.1 vs. TAIR (Arabidopsis)
Match: AT3G46000.1 (actin depolymerizing factor 2)

HSP 1 Score: 223.0 bits (567), Expect = 1.900e-58
Identity = 109/137 (79.56%), Postives = 124/137 (90.51%), Query Frame = 1


		  
Query: 60  IANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 119
           +ANAASGMAV D CKLKF+ELKAKRT+R IV+KIE+  KQVIVEK+GEP +SY+DF  ++
Sbjct: 1   MANAASGMAVHDDCKLKFMELKAKRTFRTIVYKIED--KQVIVEKLGEPEQSYDDFAASL 60

Query: 120 PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 179
           PAD+CRY +YDFD+VTAENCQKS+IFFIAWSPD AKVR KMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADDCRYCIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGIQVE 120

Query: 180 LQATDPSEMDLDVIKSR 197
           LQATDP+EM LDV KSR
Sbjct: 121 LQATDPTEMGLDVFKSR 135

The following BLAST results are available for this feature:

BLAST of Spo25287.1 vs. NCBI nr
BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
BLAST of Spo25287.1 vs. TAIR (Arabidopsis)

BLAST of Spo25287.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902111342\|gb\|KNA03751.1\|	2.1e-70	99.2	hypothetical protein SOVF_2060...	[more]
gi\|902197140\|gb\|KNA13337.1\|	2.7e-70	98.5	hypothetical protein SOVF_1179...	[more]
gi\|731318670\|ref\|XP_010669850.1\|	2.0e-65	93.4	PREDICTED: actin-depolymerizin...	[more]
gi\|731318668\|ref\|XP_010669849.1\|	2.6e-65	92.7	PREDICTED: actin-depolymerizin...	[more]
gi\|763811997\|gb\|KJB78849.1\|	2.1e-62	89.7	hypothetical protein B456_013G...	[more]

BLAST of Spo25287.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QB58_SPIOL	1.4e-70	99.2	Uncharacterized protein OS=Spi...	[more]
A0A0K9R3F8_SPIOL	1.9e-70	98.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8D098_BETVU	1.4e-65	93.4	Uncharacterized protein OS=Bet...	[more]
A0A0J8CVB6_BETVU	1.8e-65	92.7	Uncharacterized protein OS=Bet...	[more]
A0A0D2V9F2_GOSRA	1.4e-62	89.7	Uncharacterized protein OS=Gos...	[more]

BLAST of Spo25287.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ADF4_ARATH	4.6e-62	85.5	Actin-depolymerizing factor 4 ...	[more]
ADF2_PETHY	5.1e-61	83.9	Actin-depolymerizing factor 2 ...	[more]
ADF1_PETHY	1.5e-60	83.2	Actin-depolymerizing factor 1 ...	[more]
ADF1_ARATH	3.3e-60	82.4	Actin-depolymerizing factor 1 ...	[more]
ADF10_ARATH	1.1e-58	77.5	Actin-depolymerizing factor 10...	[more]

BLAST of Spo25287.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G59890.1	2.6e-63	85.5	actin depolymerizing factor 4	[more]
AT3G46010.2	2.4e-61	83.0	actin depolymerizing factor 1	[more]
AT1G01750.1	6.0e-60	77.5	actin depolymerizing factor 11	[more]
AT4G00680.1	1.5e-58	75.3	actin depolymerizing factor 8	[more]
AT3G46000.1	1.9e-58	79.5	actin depolymerizing factor 2	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 73..195 score: 4.9
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 71..198 score: 6.6
IPR002108	Actin-depolymerising factor homology domain	PROFILE	PS51263	ADF_H	coord: 66..198 score: 40
IPR017904	ADF/Cofilin/Destrin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 61..203 score: 1.6E
None	No IPR available	PANTHER	PTHR11913:SF30	ACTIN-DEPOLYMERIZING FACTOR 10-RELATED	coord: 61..203 score: 1.6E
None	No IPR available	unknown	SSF55753	Actin depolymerizing proteins	coord: 62..199 score: 6.84

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003779	actin binding