Spo25347.1 (mRNA) | SpinachBase

Overview


Name	Spo25347.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Pleckstrin (PH) domain superfamily protein
Location	chr1 : 23647963 .. 23648412 (-)
Sequence length	450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAGCCTCTGGCGATCAGCTCTAAACCGGGTAATAGGAACCGACACATCCGGATCAACCGGTCCAGAGTACGACGGGGTAGAGTTCTGGACCGAACCGGAACGATCGGGGTGGTTAACCAAACAAGGGGAGTATATCAAAACATGGCGTCGCCGCTGGTTCGTGTTAAAGAAAGGCAAGCTTTTCTGGTTCAAGGAGTCAACCGTGAACCGGTCCTCTAAACCGCGTGGCGTTATTCCGGTTTCGTCGTGTTTAACCGTGAAAGGTGCTGAGGATGTGTTGAACCGGCAGTTTGCTTTTGAGCTTTCAACCCGGTCTGATACCATGTATTTTATTGCTGATTCTGAGAAGGAGAAAGAGGATTGGATTAATTCCATTGGTCGGTCTATTGTTCAGCATTCCCGGTCTGTTACGGACCGAGAGATTGTTGATTATGATAATAAGTGA

mRNA sequence

ATGGCAAGCCTCTGGCGATCAGCTCTAAACCGGGTAATAGGAACCGACACATCCGGATCAACCGGTCCAGAGTACGACGGGGTAGAGTTCTGGACCGAACCGGAACGATCGGGGTGGTTAACCAAACAAGGGGAGTATATCAAAACATGGCGTCGCCGCTGGTTCGTGTTAAAGAAAGGCAAGCTTTTCTGGTTCAAGGAGTCAACCGTGAACCGGTCCTCTAAACCGCGTGGCGTTATTCCGGTTTCGTCGTGTTTAACCGTGAAAGGTGCTGAGGATGTGTTGAACCGGCAGTTTGCTTTTGAGCTTTCAACCCGGTCTGATACCATGTATTTTATTGCTGATTCTGAGAAGGAGAAAGAGGATTGGATTAATTCCATTGGTCGGTCTATTGTTCAGCATTCCCGGTCTGTTACGGACCGAGAGATTGTTGATTATGATAATAAGTGA

Coding sequence (CDS)

ATGGCAAGCCTCTGGCGATCAGCTCTAAACCGGGTAATAGGAACCGACACATCCGGATCAACCGGTCCAGAGTACGACGGGGTAGAGTTCTGGACCGAACCGGAACGATCGGGGTGGTTAACCAAACAAGGGGAGTATATCAAAACATGGCGTCGCCGCTGGTTCGTGTTAAAGAAAGGCAAGCTTTTCTGGTTCAAGGAGTCAACCGTGAACCGGTCCTCTAAACCGCGTGGCGTTATTCCGGTTTCGTCGTGTTTAACCGTGAAAGGTGCTGAGGATGTGTTGAACCGGCAGTTTGCTTTTGAGCTTTCAACCCGGTCTGATACCATGTATTTTATTGCTGATTCTGAGAAGGAGAAAGAGGATTGGATTAATTCCATTGGTCGGTCTATTGTTCAGCATTCCCGGTCTGTTACGGACCGAGAGATTGTTGATTATGATAATAAGTGA

Protein sequence

MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKGKLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEKEDWINSIGRSIVQHSRSVTDREIVDYDNK

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo25347	Spo25347	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo25347.1	Spo25347.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25347.1.CDS.1	Spo25347.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25347.1.exon.1	Spo25347.1.exon.1	exon

Homology

[Close]

BLAST of Spo25347.1 vs. NCBI nr
Match: gi|902227819|gb|KNA20843.1| (hypothetical protein SOVF_048510 [Spinacia oleracea])

HSP 1 Score: 308.9 bits (790), Expect = 4.800e-81
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG
Sbjct: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK
Sbjct: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTDREIVDYDNK
Sbjct: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 149

[Close]

BLAST of Spo25347.1 vs. NCBI nr
Match: gi|731315086|ref|XP_010689844.1| (PREDICTED: pleckstrin homology domain-containing protein 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 287.7 bits (735), Expect = 1.100e-74
Identity = 136/152 (89.47%), Postives = 147/152 (96.71%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGST---GPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVL 60
           M+SLWR+ALNRVIGT+T+GST   GP+YDG EFWTEPER+GWLTKQGEYIKTWRRRWF+L
Sbjct: 1   MSSLWRAALNRVIGTETTGSTTSPGPDYDGTEFWTEPERAGWLTKQGEYIKTWRRRWFIL 60

Query: 61  KKGKLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSE 120
           K+GKLFWFKES VNR+SKPRG+IPVS+CLTVKGAEDVLNRQFAFELSTRSDTMYFIADSE
Sbjct: 61  KQGKLFWFKESIVNRTSKPRGIIPVSTCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSE 120

Query: 121 KEKEDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           KEKEDWINSIGRSIVQHSRSVTDREIVDYDNK
Sbjct: 121 KEKEDWINSIGRSIVQHSRSVTDREIVDYDNK 152

[Close]

BLAST of Spo25347.1 vs. NCBI nr
Match: gi|802546931|ref|XP_012087668.1| (PREDICTED: pleckstrin homology domain-containing protein 1 [Jatropha curcas])

HSP 1 Score: 252.7 bits (644), Expect = 4.100e-64
Identity = 120/149 (80.54%), Postives = 136/149 (91.28%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWR+A   +  T T+ +   +YDGVEFW+ PER+GWLTKQGEYIKTWRRRWFVLK+G
Sbjct: 1   MASLWRAATTALTQTQTNHT---DYDGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFKESTV R+SKPRGVIPV++CLTVKGAEDVLN+Q+AFELSTR++TMYFIADSEKEK
Sbjct: 61  KLFWFKESTVTRASKPRGVIPVATCLTVKGAEDVLNKQYAFELSTRTETMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTD EIVDYD+K
Sbjct: 121 EDWINSIGRSIVQHSRSVTDSEIVDYDSK 146

[Close]

BLAST of Spo25347.1 vs. NCBI nr
Match: gi|747060497|ref|XP_011076681.1| (PREDICTED: pleckstrin homology domain-containing protein 1 [Sesamum indicum])

HSP 1 Score: 251.1 bits (640), Expect = 1.200e-63
Identity = 120/149 (80.54%), Postives = 134/149 (89.93%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWR+A+       ++ +   +YDGVEFW  PER+GWLTKQGEYIKTWRRRWFVLK+G
Sbjct: 1   MASLWRAAMG------STQANADDYDGVEFWGTPERAGWLTKQGEYIKTWRRRWFVLKQG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFKESTV R+SKPRGVIPV+SCLTVKGAEDVLNRQFAFELSTR+DTMYFIADSEKEK
Sbjct: 61  KLFWFKESTVTRASKPRGVIPVASCLTVKGAEDVLNRQFAFELSTRNDTMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTD EI+DYD++
Sbjct: 121 EDWINSIGRSIVQHSRSVTDNEILDYDSR 143

[Close]

BLAST of Spo25347.1 vs. NCBI nr
Match: gi|694320313|ref|XP_009350807.1| (PREDICTED: pleckstrin homology domain-containing protein 1-like [Pyrus x bretschneideri])

HSP 1 Score: 248.1 bits (632), Expect = 1.000e-62
Identity = 117/149 (78.52%), Postives = 133/149 (89.26%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWR+A+ +   T+       +Y+GVEFW+ PER+GWLTKQGEYIKTWRRRWFVLK+G
Sbjct: 1   MASLWRAAMGQTEQTN-------DYEGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFK+S VNR S PRGVIPV+SCLTVKGAEDVLN+Q+AFELSTRS+TMYFIADSEKEK
Sbjct: 61  KLFWFKDSAVNRGSSPRGVIPVASCLTVKGAEDVLNKQYAFELSTRSETMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTD E+VDYD+K
Sbjct: 121 EDWINSIGRSIVQHSRSVTDSEVVDYDSK 142

[Close]

BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RMY1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_048510 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 3.300e-81
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG
Sbjct: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK
Sbjct: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTDREIVDYDNK
Sbjct: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 149

[Close]

BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CWW3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g023980 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 7.900e-75
Identity = 136/152 (89.47%), Postives = 147/152 (96.71%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGST---GPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVL 60
           M+SLWR+ALNRVIGT+T+GST   GP+YDG EFWTEPER+GWLTKQGEYIKTWRRRWF+L
Sbjct: 1   MSSLWRAALNRVIGTETTGSTTSPGPDYDGTEFWTEPERAGWLTKQGEYIKTWRRRWFIL 60

Query: 61  KKGKLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSE 120
           K+GKLFWFKES VNR+SKPRG+IPVS+CLTVKGAEDVLNRQFAFELSTRSDTMYFIADSE
Sbjct: 61  KQGKLFWFKESIVNRTSKPRGIIPVSTCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSE 120

Query: 121 KEKEDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           KEKEDWINSIGRSIVQHSRSVTDREIVDYDNK
Sbjct: 121 KEKEDWINSIGRSIVQHSRSVTDREIVDYDNK 152

[Close]

BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
Match: A0A067LC64_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01298 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 2.800e-64
Identity = 120/149 (80.54%), Postives = 136/149 (91.28%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWR+A   +  T T+ +   +YDGVEFW+ PER+GWLTKQGEYIKTWRRRWFVLK+G
Sbjct: 1   MASLWRAATTALTQTQTNHT---DYDGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFKESTV R+SKPRGVIPV++CLTVKGAEDVLN+Q+AFELSTR++TMYFIADSEKEK
Sbjct: 61  KLFWFKESTVTRASKPRGVIPVATCLTVKGAEDVLNKQYAFELSTRTETMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTD EIVDYD+K
Sbjct: 121 EDWINSIGRSIVQHSRSVTDSEIVDYDSK 146

[Close]

BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
Match: A0A061FL74_THECC (Pleckstrin (PH) domain superfamily protein OS=Theobroma cacao GN=TCM_042546 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 5.900e-62
Identity = 118/150 (78.67%), Postives = 134/150 (89.33%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGP-EYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKK 60
           MASLWR+A        T+ +  P +YDGVEFW+ PER+GWLTKQGEYIKTWRRRWFVLK+
Sbjct: 1   MASLWRAA--------TALTEKPNDYDGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQ 60

Query: 61  GKLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKE 120
           GKLFWFKEST+ R S+PRGVIPV+SCLTVKGAED+LN+Q+AFELSTR++TMYFIADSEKE
Sbjct: 61  GKLFWFKESTITRGSRPRGVIPVASCLTVKGAEDILNKQYAFELSTRNETMYFIADSEKE 120

Query: 121 KEDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           KEDWINSIGRSIVQHSRSVTD EIVDYD+K
Sbjct: 121 KEDWINSIGRSIVQHSRSVTDSEIVDYDSK 142

[Close]

BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
Match: F6HCE3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g00450 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 5.900e-62
Identity = 117/149 (78.52%), Postives = 131/149 (87.92%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           M SLWR+A      +D       +Y GVEFW+ PER+GWLTKQGEYIKTWRRRWFVLK+G
Sbjct: 1   MWSLWRAATGTADDSD-------DYGGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFKEST+ R+S+PRGVIPV+SCLTVKGAEDVLN+QFAFELSTR++TMYFIADSEKEK
Sbjct: 61  KLFWFKESTITRASRPRGVIPVASCLTVKGAEDVLNKQFAFELSTRTETMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQHSRSVTD EIVDYD+K
Sbjct: 121 EDWINSIGRSIVQHSRSVTDSEIVDYDSK 142

[Close]

BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
Match: PH1_ARATH (Pleckstrin homology domain-containing protein 1 OS=Arabidopsis thaliana GN=PH1 PE=2 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 7.200e-53
Identity = 102/151 (67.55%), Postives = 120/151 (79.47%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           M S+WR A     G D S     +Y+G+EFW+ PERSGWLTKQG+YIKTWRRRWFVLK+G
Sbjct: 1   MESIWRIAT----GQDPSRE---DYEGIEFWSNPERSGWLTKQGDYIKTWRRRWFVLKRG 60

Query: 61  KLFWFKESTVN--RSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEK 120
           KL WFK+      R S PRGVI V  CLTVKGAEDV+N+ FAFELS+ S TM+FIAD+EK
Sbjct: 61  KLLWFKDQAAAGIRGSTPRGVISVGDCLTVKGAEDVVNKPFAFELSSGSYTMFFIADNEK 120

Query: 121 EKEDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EKE+WINSIGRSIVQHSRSVTD E++DYD++
Sbjct: 121 EKEEWINSIGRSIVQHSRSVTDSEVLDYDHR 144

[Close]

BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
Match: Y4775_DICDI (PH domain-containing protein DDB_G0274775 OS=Dictyostelium discoideum GN=DDB_G0274775 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 4.700e-12
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 1


		  
Query: 35  ERSGWLTKQGEYIKTWRRRWFVLKKGKLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDV 94
           +R GWLTKQG  I+TWRRRWFVLK  KLF+FK        +  G+I +     VK  +D 
Sbjct: 17  DREGWLTKQGGSIRTWRRRWFVLKGKKLFYFKS---KGDIEATGLIELEQNSFVKEEKDK 76

Query: 95  -LNRQFAFELSTRSDTMYFIADSEKEKEDWINSIGRSI 132
              +++ F + T     Y  A++E + + W+ SI R++
Sbjct: 77  DKKKKYMFTVGTSKRVFYIFAETETDMKQWMESIKRNL 111

[Close]

BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
Match: CYH3_MOUSE (Cytohesin-3 OS=Mus musculus GN=Cyth3 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.400e-10
Identity = 47/134 (35.07%), Postives = 70/134 (52.24%), Query Frame = 1


		  
Query: 23  PEYDGVEF---WTEPERSGWLTKQGEYIKTWRRRWFVLKKGKLFWFKESTVNRSSKPRGV 82
           PE DG +    +  P+R GWL K G  +KTW+RRWF+L    L++F+ +T     +PRG+
Sbjct: 251 PEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTT---DKEPRGI 310

Query: 83  IPVSSCLTVKGAEDVLNRQFAFEL-------------STRSD--------TMYFI-ADSE 132
           IP+ + L+++  ED   +   FEL              T +D         +Y I A S 
Sbjct: 311 IPLEN-LSIREVEDP-RKPNCFELYNPSHKGQVIKACKTEADGRVVEGNHVVYRISAPSP 370

[Close]

BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
Match: PKHH1_HUMAN (Pleckstrin homology domain-containing family H member 1 OS=Homo sapiens GN=PLEKHH1 PE=2 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 7.500e-10
Identity = 35/91 (38.46%), Postives = 54/91 (59.34%), Query Frame = 1


		  
Query: 35  ERSGWLTKQGEYIKTWRRRWFVLKKGKLFWFKESTVNRSSKPRGVIPVSS-CLTVKGAED 94
           E+SG+L K G  +KTW+RRWFVL++G++ ++K S  +   KP+G + ++S C  V+G   
Sbjct: 580 EKSGYLLKMGSQVKTWKRRWFVLRQGQIMYYK-SPSDVIRKPQGQVDLNSRCQIVRG--- 639

Query: 95  VLNRQFAFELSTRSDTMYFIADSEKEKEDWI 125
                  F+L +   T Y  ADS    E+WI
Sbjct: 640 --EGSQTFQLISEKKTYYLTADSPSLLEEWI 664

[Close]

BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
Match: PKHH2_MOUSE (Pleckstrin homology domain-containing family H member 2 OS=Mus musculus GN=Plekhh2 PE=1 SV=3)

HSP 1 Score: 64.3 bits (155), Expect = 1.300e-9
Identity = 39/91 (42.86%), Postives = 55/91 (60.44%), Query Frame = 1


		  
Query: 35  ERSGWLTKQGEYIKTWRRRWFVLKKGKLFWFKESTVNRSSKPRGVIPVS-SCLTVKGAED 94
           E+SG+L K    +KTW+RRWFVLK G+L ++K S  +   KP+G I +S SC  ++G   
Sbjct: 704 EKSGYLLKMSVRVKTWKRRWFVLKGGELLYYK-SPSDVIRKPQGHIELSASCSILRGD-- 763

Query: 95  VLNRQFAFELSTRSDTMYFIADSEKEKEDWI 125
             N+Q   +L+T   T Y  ADS    E+WI
Sbjct: 764 --NKQ-TVQLATEKHTYYLTADSPNILEEWI 788

[Close]

BLAST of Spo25347.1 vs. TAIR (Arabidopsis)
Match: AT5G05710.1 (Pleckstrin homology (PH) domain superfamily protein)

HSP 1 Score: 236.5 bits (602), Expect = 1.100e-62
Identity = 116/149 (77.85%), Postives = 129/149 (86.58%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           MASLWR+    VIG   + S   +Y GVEFW+ PER+GWLTKQGEYIKTWRRRWFVLK+G
Sbjct: 1   MASLWRA----VIGGQNNNSE--DYGGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQG 60

Query: 61  KLFWFKESTVNRSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEKEK 120
           KLFWFK+S V R S+PRGV+PV SCLT KGAEDVLN+Q AFELSTR++TMYFIADSEKEK
Sbjct: 61  KLFWFKDSDVTRVSRPRGVVPVESCLTAKGAEDVLNKQNAFELSTRNETMYFIADSEKEK 120

Query: 121 EDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EDWINSIGRSIVQ+SRSVTD EIVDYDNK
Sbjct: 121 EDWINSIGRSIVQNSRSVTDSEIVDYDNK 143

[Close]

BLAST of Spo25347.1 vs. TAIR (Arabidopsis)
Match: AT2G29700.1 (pleckstrin homologue 1)

HSP 1 Score: 208.0 bits (528), Expect = 4.000e-54
Identity = 102/151 (67.55%), Postives = 120/151 (79.47%), Query Frame = 1


		  
Query: 1   MASLWRSALNRVIGTDTSGSTGPEYDGVEFWTEPERSGWLTKQGEYIKTWRRRWFVLKKG 60
           M S+WR A     G D S     +Y+G+EFW+ PERSGWLTKQG+YIKTWRRRWFVLK+G
Sbjct: 1   MESIWRIAT----GQDPSRE---DYEGIEFWSNPERSGWLTKQGDYIKTWRRRWFVLKRG 60

Query: 61  KLFWFKESTVN--RSSKPRGVIPVSSCLTVKGAEDVLNRQFAFELSTRSDTMYFIADSEK 120
           KL WFK+      R S PRGVI V  CLTVKGAEDV+N+ FAFELS+ S TM+FIAD+EK
Sbjct: 61  KLLWFKDQAAAGIRGSTPRGVISVGDCLTVKGAEDVVNKPFAFELSSGSYTMFFIADNEK 120

Query: 121 EKEDWINSIGRSIVQHSRSVTDREIVDYDNK 150
           EKE+WINSIGRSIVQHSRSVTD E++DYD++
Sbjct: 121 EKEEWINSIGRSIVQHSRSVTDSEVLDYDHR 144

The following BLAST results are available for this feature:

BLAST of Spo25347.1 vs. NCBI nr
BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
BLAST of Spo25347.1 vs. TAIR (Arabidopsis)

BLAST of Spo25347.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902227819\|gb\|KNA20843.1\|	4.8e-81	100.	hypothetical protein SOVF_0485...	[more]
gi\|731315086\|ref\|XP_010689844.1\|	1.1e-74	89.4	PREDICTED: pleckstrin homology...	[more]
gi\|802546931\|ref\|XP_012087668.1\|	4.1e-64	80.5	PREDICTED: pleckstrin homology...	[more]
gi\|747060497\|ref\|XP_011076681.1\|	1.2e-63	80.5	PREDICTED: pleckstrin homology...	[more]
gi\|694320313\|ref\|XP_009350807.1\|	1.0e-62	78.5	PREDICTED: pleckstrin homology...	[more]

BLAST of Spo25347.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RMY1_SPIOL	3.3e-81	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CWW3_BETVU	7.9e-75	89.4	Uncharacterized protein OS=Bet...	[more]
A0A067LC64_JATCU	2.8e-64	80.5	Uncharacterized protein OS=Jat...	[more]
A0A061FL74_THECC	5.9e-62	78.6	Pleckstrin (PH) domain superfa...	[more]
F6HCE3_VITVI	5.9e-62	78.5	Putative uncharacterized prote...	[more]

BLAST of Spo25347.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
PH1_ARATH	7.2e-53	67.5	Pleckstrin homology domain-con...	[more]
Y4775_DICDI	4.7e-12	38.7	PH domain-containing protein D...	[more]
CYH3_MOUSE	3.4e-10	35.0	Cytohesin-3 OS=Mus musculus GN...	[more]
PKHH1_HUMAN	7.5e-10	38.4	Pleckstrin homology domain-con...	[more]
PKHH2_MOUSE	1.3e-9	42.8	Pleckstrin homology domain-con...	[more]

BLAST of Spo25347.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G05710.1	1.1e-62	77.8	Pleckstrin homology (PH) domai...	[more]
AT2G29700.1	4.0e-54	67.5	pleckstrin homologue 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001849	Pleckstrin homology domain	PFAM	PF00169	PH	coord: 35..130 score: 1.3
IPR001849	Pleckstrin homology domain	SMART	SM00233	PH_update	coord: 34..134 score: 6.1
IPR001849	Pleckstrin homology domain	PROFILE	PS50003	PH_DOMAIN	coord: 33..132 score: 19
IPR011993	PH domain-like	GENE3D	2.30.29.30		coord: 33..139 score: 1.9
IPR011993	PH domain-like	unknown	SSF50729	PH domain-like	coord: 30..132 score: 9.82
None	No IPR available	PANTHER	PTHR22903	PLEKHH PROTEIN	coord: 1..149 score: 1.1
None	No IPR available	PANTHER	PTHR22903:SF18	SUBFAMILY NOT NAMED	coord: 1..149 score: 1.1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005543	phospholipid binding