Spo26697.1 (mRNA) | SpinachBase

Overview


Name	Spo26697.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Iron-sulfur cluster assembly protein IscA
Location	chr3 : 49792952 .. 49795670 (-)
Sequence length	1273

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTATTACGTACGGTAACAAATAGCAACCACAAAGCTTTAGTTTGCGAAATCAAAAGACAATCAGATTCCAAAGCCTTGGAAAATAGCGTCGGACTGCAAATCCCCAAAAAACTCTAAAATAATGGCGTCTTCTTCATTGACACTTCGGTGTTCATCAACAATTTGCAATTTACCTAGTAATTCTTCTGCTTCATTGAGCTTCAATCGTAGCCCATTTTCAGTTTCTTTCCCATCAAATCTCAAACGCCTTAATCGTCTCTCTATTCGATCATCTTCTTCAGCTTCTGGTACTCTCTTCATCTTTGTATTTACTCGATGTTGTTAGGATGTTATTGTGAATATAATGATACGGCTCACACTAATACGGCTGTTTGACTCTCATATACCCTTCATATCCTTTAGGAGTTATTCTTGTATTTAATTTTGTTTCGTGGGTTATATTGAAATTCTTGTGGAGTGTTCTATAGATGGAACTTTTTGATTAATTTGATACTGCTTGGCTACTATATGATGTTATTTTGATTGAATTTCGGTAAATTTAATTGATTATATTTTGGATTACTCAGCTAATTTGAGCATGTAGGTCTTTGATTTTGATTATATTTGCTGATGAGCAACTTTTTGGTTTCGAACCGATTTGATCTTGAGCTTGAATAACCCCCTTATATGTTGAGATGTTCTTAGAATTCTGATTACGGTAGGTTTAGGGTTCATGACTCTTTGATTCGTGTGGACTAGTTTCCCCCCAAATATACGAAATGCAAACTGATTAGCCGGAAGCCCTCGAAATTGTTACGGTCCATTGCAAGGTATGGGACAGTCCCTTATTGGTCATACGGGAGGCTGATGTGGGGTTTATATAGGATTATGGGCTCTCTTCCCCTTGAGCTAGCTTTTGCAGATGGCTCTCCTATGGTCTTTTGTCCTTTATCAATGGTATCAGAGCCGACCACCGCTCCTAGCACCACATGTGACTGATGGTGGTGACGCTGGTGCTCGTCCGGGGCTAGGAACCTTCTCTTCTAAGCTAGCTTTTGGGGATGACTCTCCTATGGTCCTGTACCACAAATCGTTAATTTCCAAACATAAGTTAGTCTATAAGGATGGGTCTAGGTGATTTATCCCATATCACCCCTAAGAGAGGGAAAAGAAAATCAATTTCAAGCACATGCTTGAGCGGAAGCGGAACAATTGTGGGAAAAGCACAAAAACAAAGCAATTTGCTTCTTGATTTAAGACAATTTAATAAACAAATGTGGATGTCCATGATTGTAGACAATATTATGATCAAATGTGATGTTTTCCCCTAGGGTTGGCTATTGACAAATCCAACAACAAACTAATCAGTACAAATAGAGGTTGTTTGTGTCCAAAACAAGCTGATATTGTCCAAGACTTCCTCGTTCTCCTCCACTCTTGTAAATTGTGTCTTTTCTGAATGGTCTTTTTAACCCATGTCCACATGCATTTGCTGGTAATTAGTTGTCACTTGAAAAATCTATTGGGTTAGTGACACTGATATTCTGTTCTTCACCGCAGTATCTCTTACTATTTCTAACTTACTTTTGGTATGCATTGTTAAGTTTCCACAGCACCAGTTGAGGGTTTGGCTCCGGCAATATCTCTCACTGACAATGCAATGAAGCATCTGAAAAGAATGATGTCAGATCGGAATGAGGAGTTGTGCTTAAGAGTCGGTGTTAAACAAGGTGGATGCTCTGGCATGTCTTACGTAATGGATTTTGAGAGTACAGATAACACAAAACCAGATGATTCTGTCATCGAGATCCATGGATTTATAATTGGTAAGTTGTTAAAAGTTTAAAAAGCATCAACTCAATCTTCTCCTCTATTCCTATACAAGTGAAAGGAGAGAAATATGTGTGAAGAAATTGCTTGTAGTCTTGATCCATGCATTTAGTTTCTCATCTACTGTAGTTGTGATTCATTTGCAGTTTGTGATCCGAAAAGTCTCCTATTCATATATGGAATGCAGCTTGACTATAGTGATGCGCTTATCGGTGGAGGCTTCTCCTTCCAAAACCCAAATGCATCAAAGACTTGCAGCTGTGGAAAATCGTTTGCAGCGTAGGCTTGAAAATATTATATAGACAAAAACATGAGCAAATCATGTACGGTGGTGTAGCTTGGATTTGTTGGGATTGTGGATAAAAATGGGCATCGTGTTAGAAGGATATATAGTCTTTTATAGTGAATTCTGTCAAATCGTCCTCAATTCTTAACATGATATTGTTCTATTTTTTCTCACACTTTGCATTTTTCATCCACTAACATTGAGTGCTAGGTTTCACACATCCATATCCACTGTCCCCACCATGGTTTGCAGTTTTATTAAAGAACTGGTCATCTGTTTTGCTGAACATTGAATCCATCCAGATCCAGCCATGTTTATATGACTTTGATGCTCCGTTCTGATTGTGCAGTCTCTAAATAAGTGTTCTCGTTCTTCACAGAGCTTGCACATTGAATCCACAACAAATTGATCTTCCATATCCATTTATTATTCATCTTACCCTTCCAAATTATCTTCCTTATATCCCTGCTTTCTGCTGTCCCCGTTACTGACTCTTCATTCTGAATTTTATACCCACTCCTCAAAGAGTAGTTCCCATGTTTTTCATATTTCCACCTAATAGTGTCCTTAGTGCCAATATCCAGGGGGGCAATAGACAAGTCAGACTATCATGATAATGAGC

mRNA sequence

GAGTATTACGTACGGTAACAAATAGCAACCACAAAGCTTTAGTTTGCGAAATCAAAAGACAATCAGATTCCAAAGCCTTGGAAAATAGCGTCGGACTGCAAATCCCCAAAAAACTCTAAAATAATGGCGTCTTCTTCATTGACACTTCGGTGTTCATCAACAATTTGCAATTTACCTAGTAATTCTTCTGCTTCATTGAGCTTCAATCGTAGCCCATTTTCAGTTTCTTTCCCATCAAATCTCAAACGCCTTAATCGTCTCTCTATTCGATCATCTTCTTCAGCTTCTGTTTCCACAGCACCAGTTGAGGGTTTGGCTCCGGCAATATCTCTCACTGACAATGCAATGAAGCATCTGAAAAGAATGATGTCAGATCGGAATGAGGAGTTGTGCTTAAGAGTCGGTGTTAAACAAGGTGGATGCTCTGGCATGTCTTACGTAATGGATTTTGAGAGTACAGATAACACAAAACCAGATGATTCTGTCATCGAGATCCATGGATTTATAATTGTTTGTGATCCGAAAAGTCTCCTATTCATATATGGAATGCAGCTTGACTATAGTGATGCGCTTATCGGTGGAGGCTTCTCCTTCCAAAACCCAAATGCATCAAAGACTTGCAGCTGTGGAAAATCGTTTGCAGCGTAGGCTTGAAAATATTATATAGACAAAAACATGAGCAAATCATGTACGGTGGTGTAGCTTGGATTTGTTGGGATTGTGGATAAAAATGGGCATCGTGTTAGAAGGATATATAGTCTTTTATAGTGAATTCTGTCAAATCGTCCTCAATTCTTAACATGATATTGTTCTATTTTTTCTCACACTTTGCATTTTTCATCCACTAACATTGAGTGCTAGGTTTCACACATCCATATCCACTGTCCCCACCATGGTTTGCAGTTTTATTAAAGAACTGGTCATCTGTTTTGCTGAACATTGAATCCATCCAGATCCAGCCATGTTTATATGACTTTGATGCTCCGTTCTGATTGTGCAGTCTCTAAATAAGTGTTCTCGTTCTTCACAGAGCTTGCACATTGAATCCACAACAAATTGATCTTCCATATCCATTTATTATTCATCTTACCCTTCCAAATTATCTTCCTTATATCCCTGCTTTCTGCTGTCCCCGTTACTGACTCTTCATTCTGAATTTTATACCCACTCCTCAAAGAGTAGTTCCCATGTTTTTCATATTTCCACCTAATAGTGTCCTTAGTGCCAATATCCAGGGGGGCAATAGACAAGTCAGACTATCATGATAATGAGC

Coding sequence (CDS)

ATGGCGTCTTCTTCATTGACACTTCGGTGTTCATCAACAATTTGCAATTTACCTAGTAATTCTTCTGCTTCATTGAGCTTCAATCGTAGCCCATTTTCAGTTTCTTTCCCATCAAATCTCAAACGCCTTAATCGTCTCTCTATTCGATCATCTTCTTCAGCTTCTGTTTCCACAGCACCAGTTGAGGGTTTGGCTCCGGCAATATCTCTCACTGACAATGCAATGAAGCATCTGAAAAGAATGATGTCAGATCGGAATGAGGAGTTGTGCTTAAGAGTCGGTGTTAAACAAGGTGGATGCTCTGGCATGTCTTACGTAATGGATTTTGAGAGTACAGATAACACAAAACCAGATGATTCTGTCATCGAGATCCATGGATTTATAATTGTTTGTGATCCGAAAAGTCTCCTATTCATATATGGAATGCAGCTTGACTATAGTGATGCGCTTATCGGTGGAGGCTTCTCCTTCCAAAACCCAAATGCATCAAAGACTTGCAGCTGTGGAAAATCGTTTGCAGCGTAG

Protein sequence

MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo26697	Spo26697	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo26697.1	Spo26697.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo26697.1.utr5p.1	Spo26697.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo26697.1.CDS.3	Spo26697.1.CDS.3	CDS
Spo26697.1.CDS.2	Spo26697.1.CDS.2	CDS
Spo26697.1.CDS.1	Spo26697.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo26697.1.utr3p.1	Spo26697.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo26697.1.exon.3	Spo26697.1.exon.3	exon
Spo26697.1.exon.2	Spo26697.1.exon.2	exon
Spo26697.1.exon.1	Spo26697.1.exon.1	exon

Homology

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BLAST of Spo26697.1 vs. NCBI nr
Match: gi|902100019|gb|KNA03497.1| (hypothetical protein SOVF_208650 [Spinacia oleracea])

HSP 1 Score: 336.7 bits (862), Expect = 2.500e-89
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


		  
Query: 1   MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAP 60
           MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAP
Sbjct: 1   MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAP 60

Query: 61  VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS 120
           VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS
Sbjct: 61  VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS 120

Query: 121 VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
           VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA
Sbjct: 121 VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 174

[Close]

BLAST of Spo26697.1 vs. NCBI nr
Match: gi|731339822|ref|XP_010681065.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 266.2 bits (679), Expect = 4.100e-68
Identity = 135/171 (78.95%), Postives = 153/171 (89.47%), Query Frame = 1


		  
Query: 3   SSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVE 62
           +SS++LRCSSTIC LP+NSS SLS N   FS+SFP NLKR+ RLSI+SSS ASVS  PVE
Sbjct: 2   ASSVSLRCSSTICKLPTNSSPSLSLNHGAFSISFPLNLKRITRLSIQSSS-ASVSATPVE 61

Query: 63  GLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVI 122
           GLAPAISLTDNA+KHLKR+ +DRNE++CLRVGVKQGGCSGMSY MDFES +N KP+DSV+
Sbjct: 62  GLAPAISLTDNALKHLKRIRADRNEDVCLRVGVKQGGCSGMSYTMDFESKENMKPEDSVM 121

Query: 123 EIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFA 174
           E++GF IV DPKSLLF+YGMQLDYSDALIGGGFSF NPNASKTCSCGKSFA
Sbjct: 122 ELNGFTIVSDPKSLLFLYGMQLDYSDALIGGGFSFNNPNASKTCSCGKSFA 171

[Close]

BLAST of Spo26697.1 vs. NCBI nr
Match: gi|731339824|ref|XP_010681066.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 261.5 bits (667), Expect = 1.000e-66
Identity = 132/171 (77.19%), Postives = 151/171 (88.30%), Query Frame = 1


		  
Query: 3   SSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVE 62
           +SS++LRCSSTIC LP+NSS SLS N   FS+SFP NLKR+ RLSI+SSS+   S  PVE
Sbjct: 2   ASSVSLRCSSTICKLPTNSSPSLSLNHGAFSISFPLNLKRITRLSIQSSSA---SATPVE 61

Query: 63  GLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVI 122
           GLAPAISLTDNA+KHLKR+ +DRNE++CLRVGVKQGGCSGMSY MDFES +N KP+DSV+
Sbjct: 62  GLAPAISLTDNALKHLKRIRADRNEDVCLRVGVKQGGCSGMSYTMDFESKENMKPEDSVM 121

Query: 123 EIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFA 174
           E++GF IV DPKSLLF+YGMQLDYSDALIGGGFSF NPNASKTCSCGKSFA
Sbjct: 122 ELNGFTIVSDPKSLLFLYGMQLDYSDALIGGGFSFNNPNASKTCSCGKSFA 169

[Close]

BLAST of Spo26697.1 vs. NCBI nr
Match: gi|470103403|ref|XP_004288128.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Fragaria vesca subsp. vesca])

HSP 1 Score: 213.4 bits (542), Expect = 3.200e-52
Identity = 112/178 (62.92%), Postives = 141/178 (79.21%), Query Frame = 1


		  
Query: 1   MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSF---PSNLKRLNRLSIRSSSSASVS 60
           MA S++T  C S++  LP+ S ++ S + +  SVSF    ++L R  R+SIR   S S S
Sbjct: 2   MAFSAITTHCPSSLLRLPATSPSASSSSSARNSVSFRFSSTSLSRGRRISIR---SVSTS 61

Query: 61  TAP-VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTK 120
            AP  EG+APAIS+T+NA+KH  +M ++RNE+LCLR+GVKQGGCSGMSY MDFES +N +
Sbjct: 62  AAPKSEGIAPAISVTENALKHFNKMRAERNEDLCLRIGVKQGGCSGMSYTMDFESKENAR 121

Query: 121 PDDSVIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
           PDDS+IE +GF IVCDPKSLLF+YGMQLDYSDALIGGGFSF+NPNA++TC CGKSFAA
Sbjct: 122 PDDSIIEYNGFEIVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA 176

[Close]

BLAST of Spo26697.1 vs. NCBI nr
Match: gi|697118566|ref|XP_009613213.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic isoform X1 [Nicotiana tomentosiformis])

HSP 1 Score: 213.4 bits (542), Expect = 3.200e-52
Identity = 108/171 (63.16%), Postives = 134/171 (78.36%), Query Frame = 1


		  
Query: 5   SLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVEG- 64
           S T RCSS++C   +NS+ S +   S  S+SF S+    NR    S  SA++  AP  G 
Sbjct: 4   SATFRCSSSLCRPLNNSTISRALPHSSSSLSFRSSPSLFNRKKSFSIRSATLEAAPASGG 63

Query: 65  LAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIE 124
           +APAISLTDNA+KHL +M ++RNE+LCLR+GVKQGGCSGMSY M+FE  +N +PDDS+IE
Sbjct: 64  VAPAISLTDNALKHLNKMRNERNEDLCLRIGVKQGGCSGMSYTMEFEKRENARPDDSIIE 123

Query: 125 IHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
            +GF IVCDPKSLLF++GMQLDYSDALIGGGFSF+NPNA++TC CGKSFAA
Sbjct: 124 YNGFNIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA 174

[Close]

BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q894_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_208650 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.700e-89
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


		  
Query: 1   MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAP 60
           MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAP
Sbjct: 1   MASSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAP 60

Query: 61  VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS 120
           VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS
Sbjct: 61  VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS 120

Query: 121 VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
           VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA
Sbjct: 121 VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 174

[Close]

BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EZI1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g139130 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 2.900e-68
Identity = 135/171 (78.95%), Postives = 153/171 (89.47%), Query Frame = 1


		  
Query: 3   SSSLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVE 62
           +SS++LRCSSTIC LP+NSS SLS N   FS+SFP NLKR+ RLSI+SSS ASVS  PVE
Sbjct: 2   ASSVSLRCSSTICKLPTNSSPSLSLNHGAFSISFPLNLKRITRLSIQSSS-ASVSATPVE 61

Query: 63  GLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVI 122
           GLAPAISLTDNA+KHLKR+ +DRNE++CLRVGVKQGGCSGMSY MDFES +N KP+DSV+
Sbjct: 62  GLAPAISLTDNALKHLKRIRADRNEDVCLRVGVKQGGCSGMSYTMDFESKENMKPEDSVM 121

Query: 123 EIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFA 174
           E++GF IV DPKSLLF+YGMQLDYSDALIGGGFSF NPNASKTCSCGKSFA
Sbjct: 122 ELNGFTIVSDPKSLLFLYGMQLDYSDALIGGGFSFNNPNASKTCSCGKSFA 171

[Close]

BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
Match: K4C1K1_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.800e-52
Identity = 109/171 (63.74%), Postives = 132/171 (77.19%), Query Frame = 1


		  
Query: 5   SLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVEG- 64
           S TLRCSS++     NS+ S    +S  S+SF S+    NR    S  SA++  AP  G 
Sbjct: 4   SATLRCSSSLHRPSKNSTISSVLPQSSSSISFRSSPSFFNRKKSLSIRSATLEAAPASGG 63

Query: 65  LAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIE 124
           +APAISLTDNA+ HL +M SDRNE+LCLR+GVKQGGCSGMSY M+FE  +N +PDDS+IE
Sbjct: 64  VAPAISLTDNALMHLNKMRSDRNEDLCLRIGVKQGGCSGMSYTMEFEKRENARPDDSIIE 123

Query: 125 IHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
            +GF+IVCDPKSLLFI+GMQLDYSDALIGGGFSF+NPNA++TC CGKSF A
Sbjct: 124 YNGFVIVCDPKSLLFIFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFTA 174

[Close]

BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
Match: A0A0V0H9U7_SOLCH (Putative iron-sulfur assembly protein IscA, chloroplastic-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.200e-51
Identity = 108/171 (63.16%), Postives = 130/171 (76.02%), Query Frame = 1


		  
Query: 5   SLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVEG- 64
           S TLRCSS+      NS+ S    +S  S SF S+    NR    S  SA++  AP  G 
Sbjct: 4   SATLRCSSSQYRPSKNSTISSVLRQSSSSXSFRSSPSFFNRKKTLSIRSATLEAAPASGG 63

Query: 65  LAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIE 124
           +APAISLTDNA+ HL +M +DRNE+LCLR+GVKQGGCSGMSY M+FE  +N +PDDS+IE
Sbjct: 64  VAPAISLTDNALMHLNKMRNDRNEDLCLRIGVKQGGCSGMSYTMEFEKRENARPDDSIIE 123

Query: 125 IHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
            +GF+IVCDPKSLLFI+GMQLDYSDALIGGGFSF+NPNA++TC CGKSF A
Sbjct: 124 YNGFVIVCDPKSLLFIFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFTA 174

[Close]

BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
Match: M1CYC4_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400030100 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.200e-51
Identity = 107/171 (62.57%), Postives = 131/171 (76.61%), Query Frame = 1


		  
Query: 5   SLTLRCSSTICNLPSNSSASLSFNRSPFSVSFPSNLKRLNRLSIRSSSSASVSTAPVEG- 64
           S TLRCSS++     NS+ S    +S  S+SF S+    NR    S  SA++  AP  G 
Sbjct: 4   SATLRCSSSLYRPSKNSTISSVLRQSSSSLSFRSSPSFFNRKKTLSIRSATLEAAPASGG 63

Query: 65  LAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIE 124
           +APAISLTDNA+ HL +M +DRN +LCLR+GVKQGGCSGMSY M+FE  +N +PDDS+IE
Sbjct: 64  VAPAISLTDNALMHLNKMRNDRNVDLCLRIGVKQGGCSGMSYTMEFEKRENARPDDSIIE 123

Query: 125 IHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
            +GF+IVCDPKSLLFI+GMQLDYSDALIGGGFSF+NPNA++TC CGKSF A
Sbjct: 124 YNGFVIVCDPKSLLFIFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFTA 174

[Close]

BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
Match: ISCAP_ARATH (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana GN=ISCA PE=2 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 1.200e-51
Identity = 108/161 (67.08%), Postives = 126/161 (78.26%), Query Frame = 1


		  
Query: 21  SSASLSFNRSPFSVSFPS------NLKRLNRLSIRSSSSASVSTAP-VEGLAPAISLTDN 80
           S+ SL    S  S+SFPS      NL R NRLS+RS   ASV  AP +EGL PAISL++N
Sbjct: 21  SNTSLRSVVSCNSISFPSLSYVNLNLNRRNRLSVRS---ASVPAAPAMEGLKPAISLSEN 80

Query: 81  AMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIEIHGFIIVCDP 140
           A+KHL +M S+R E+LCLR+GVKQGGCSGMSY MDFE+  N +PDDS IE  GF IVCDP
Sbjct: 81  ALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDP 140

Query: 141 KSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
           KS+LF++GMQLDYSDALIGGGFSF NPNA++TC CGKSFAA
Sbjct: 141 KSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKSFAA 178

[Close]

BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
Match: Y1417_SYNY3 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1417 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.500e-33
Identity = 64/108 (59.26%), Postives = 81/108 (75.00%), Query Frame = 1


		  
Query: 65  APAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIEI 124
           A  I L+D A+KHL  +   + ++LCLRVGV+QGGCSGMSY+MDFE  +     D V + 
Sbjct: 9   AKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHDEVFDY 68

Query: 125 HGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSF 173
            GF I+CD KSLL++YG+ LDYS+ALIGGGF F NPNA++TC CGKSF
Sbjct: 69  EGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

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BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
Match: YCF83_PORPU (Uncharacterized protein ycf83 OS=Porphyra purpurea GN=ycf83 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.100e-32
Identity = 61/106 (57.55%), Postives = 82/106 (77.36%), Query Frame = 1


		  
Query: 68  ISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIEIHGF 127
           I+LT+ A + +K + S+ + E+CLR+GVKQGGCSGMSY M+FES  + +  D V+ +  F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 128 IIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFA 174
           ++ CDPKSLL++YG+ LDYS  LIGGGF F NPNAS+TC CGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
Match: YCF83_PYRYE (Uncharacterized protein ycf83 OS=Pyropia yezoensis GN=ycf83 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 5.800e-30
Identity = 59/113 (52.21%), Postives = 80/113 (70.80%), Query Frame = 1


		  
Query: 61  VEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDS 120
           +EG    I++T  A+K +  + +D   ++ LR+GV+QGGCSGMSY M+FE  D  K  D 
Sbjct: 1   MEGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDE 60

Query: 121 VIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFA 174
            + +  F +VCDPKSLL++YG+ LD+S  LIGGGF F NPNAS+TC CGKSF+
Sbjct: 61  RLRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
Match: YCF83_GALSU (Uncharacterized protein ycf83 OS=Galdieria sulphuraria GN=ycf83 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 8.700e-26
Identity = 53/107 (49.53%), Postives = 75/107 (70.09%), Query Frame = 1


		  
Query: 68  ISLTDNAMKHLKRMMSDRNEE-LCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIEIHG 127
           I++T +A+ HL  +    +E+ L +R+G+KQGGCSG+SY M +E   N   +D V +   
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 128 FIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFA 174
           F +VCD KS+L++YG+ LDYS +LI GGF F NPNA +TC CGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

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BLAST of Spo26697.1 vs. TAIR (Arabidopsis)
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein)

HSP 1 Score: 204.1 bits (518), Expect = 6.800e-53
Identity = 108/161 (67.08%), Postives = 126/161 (78.26%), Query Frame = 1


		  
Query: 21  SSASLSFNRSPFSVSFPS------NLKRLNRLSIRSSSSASVSTAP-VEGLAPAISLTDN 80
           S+ SL    S  S+SFPS      NL R NRLS+RS   ASV  AP +EGL PAISL++N
Sbjct: 21  SNTSLRSVVSCNSISFPSLSYVNLNLNRRNRLSVRS---ASVPAAPAMEGLKPAISLSEN 80

Query: 81  AMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDFESTDNTKPDDSVIEIHGFIIVCDP 140
           A+KHL +M S+R E+LCLR+GVKQGGCSGMSY MDFE+  N +PDDS IE  GF IVCDP
Sbjct: 81  ALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDP 140

Query: 141 KSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSFAA 175
           KS+LF++GMQLDYSDALIGGGFSF NPNA++TC CGKSFAA
Sbjct: 141 KSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKSFAA 178

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BLAST of Spo26697.1 vs. TAIR (Arabidopsis)
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 79.0 bits (193), Expect = 3.300e-15
Identity = 48/127 (37.80%), Postives = 69/127 (54.33%), Query Frame = 1


		  
Query: 47  SIRSSSSASVSTAPVEGLAPAISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYV 106
           +I+ +SS+S S+ P       + L+DN ++ +K + S   E+  LR+GV+ GGCSG  Y 
Sbjct: 31  AIKEASSSS-SSQPESSSNDVVHLSDNCIRRMKELQSSEPEKKMLRLGVETGGCSGFQY- 90

Query: 107 MDFESTDNTKPDDSVIEIHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQ-NPNASKT 166
             FE  + T PDD V E +G  +V D  S   + G  +DY + LI   F    NP+A   
Sbjct: 91  -KFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVKGATIDYEEELIRAAFVVAVNPSAVGG 150

Query: 167 CSCGKSF 173
           CSC  SF
Sbjct: 151 CSCKSSF 154

[Close]

BLAST of Spo26697.1 vs. TAIR (Arabidopsis)
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 70.9 bits (172), Expect = 9.000e-13
Identity = 35/109 (32.11%), Postives = 62/109 (56.88%), Query Frame = 1


		  
Query: 68  ISLTDNAMKHLKRMMSDRNEELCLRVGVKQGGCSGMSYVMDF----ESTDNTKPDDSVIE 127
           ++LTD A   +  ++  + ++  LR+GVK  GC+G+SY +++       D     D ++E
Sbjct: 23  LTLTDEAASRVHHLL-QQRQKPFLRLGVKARGCNGLSYTLNYAGKTSGNDEKGKFDELVE 82

Query: 128 IHGFIIVCDPKSLLFIYGMQLDYSDALIGGGFSFQNPNASKTCSCGKSF 173
             G  I+ +PK+L+ + G ++D+ D  +   F F NPN+   C CG+SF
Sbjct: 83  EKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130

The following BLAST results are available for this feature:

BLAST of Spo26697.1 vs. NCBI nr
BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
BLAST of Spo26697.1 vs. TAIR (Arabidopsis)

BLAST of Spo26697.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902100019\|gb\|KNA03497.1\|	2.5e-89	100.	hypothetical protein SOVF_2086...	[more]
gi\|731339822\|ref\|XP_010681065.1\|	4.1e-68	78.9	PREDICTED: iron-sulfur assembl...	[more]
gi\|731339824\|ref\|XP_010681066.1\|	1.0e-66	77.1	PREDICTED: iron-sulfur assembl...	[more]
gi\|470103403\|ref\|XP_004288128.1\|	3.2e-52	62.9	PREDICTED: iron-sulfur assembl...	[more]
gi\|697118566\|ref\|XP_009613213.1\|	3.2e-52	63.1	PREDICTED: iron-sulfur assembl...	[more]

BLAST of Spo26697.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q894_SPIOL	1.7e-89	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8EZI1_BETVU	2.9e-68	78.9	Uncharacterized protein OS=Bet...	[more]
K4C1K1_SOLLC	3.8e-52	63.7	Uncharacterized protein OS=Sol...	[more]
A0A0V0H9U7_SOLCH	3.2e-51	63.1	Putative iron-sulfur assembly ...	[more]
M1CYC4_SOLTU	3.2e-51	62.5	Uncharacterized protein OS=Sol...	[more]

BLAST of Spo26697.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ISCAP_ARATH	1.2e-51	67.0	Iron-sulfur assembly protein I...	[more]
Y1417_SYNY3	2.5e-33	59.2	Uncharacterized protein slr141...	[more]
YCF83_PORPU	2.1e-32	57.5	Uncharacterized protein ycf83 ...	[more]
YCF83_PYRYE	5.8e-30	52.2	Uncharacterized protein ycf83 ...	[more]
YCF83_GALSU	8.7e-26	49.5	Uncharacterized protein ycf83 ...	[more]

BLAST of Spo26697.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G10500.1	6.8e-53	67.0	chloroplast-localized ISCA-lik...	[more]
AT5G03905.1	3.3e-15	37.8	Iron-sulphur cluster biosynthe...	[more]
AT2G16710.3	9.0e-13	32.1	Iron-sulphur cluster biosynthe...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	GENE3D	2.60.300.12		coord: 68..163 score: 2.6
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 68..170 score: 2.0
IPR000361	FeS cluster biogenesis	unknown	SSF89360	HesB-like domain	coord: 67..162 score: 2.09
IPR016092	FeS cluster insertion protein	TIGRFAMs	TIGR00049	TIGR00049	coord: 68..173 score: 8.2
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 155..172 scor
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 46..173 score: 2.0

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0042744	hydrogen peroxide catabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity
molecular_function	GO:0046872	metal ion binding