Spo27157.1 (mRNA) | SpinachBase

Overview


Name	Spo27157.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	L-Galactono-1,4-lactone dehydrogenase
Location	SpoScf_11178 : 3 .. 2122 (-)
Sequence length	888

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACCGAGTAACCAATCTCCGACGATTTATTCCCTCTCTCCTCCACCACCATCACCACCGCCATTTACACCAAAACACCAACCCGATCAAATCCCTCACCTCCGCAAACTCAACCATCCTCCAAAACCCCGCCTTACACCCCCGCTCATTCTCTTCTCTCTCCTCTGACCCTACGGCCTCCACCACCGCCGAAATCCGCAAATACATCGGATACTCCCTCCTCCTCCTCGGTTGCGGCGTTGGAACCTACTATTCCTTCCCTTTCCCGGAAAACGCCAAGCACAAAAAAGCCCAACTCTTCAAATACGCCCCTCTCCCCGAAGATCTTCATACCATCTCTAATTGGAGTGGTACCCATGAGGTCCATACCCGCAATTTCATTCAACCGGAATCGGTTTCGGACCTCGAAAAACTTGTCCAAGAAGCCCATCAAAAGTCCCAGAAGATTCGGCCTGTTGGGTCGGGTCTTTCTCCAAATGGGGTTGGGCTTTCTCGGGCTGGCATGGTGAATTTGGGCTTGTTGGATAAGGTATTGGAGGTTGATAAGGAGAAGAAGACTGTTAGAGTTGAAGCTGGTATTAGAGTTCAGCAGCTTGTTGATCAAATTAAGGAGTACGGACTTACTTTGCAAAATTTTGCTTCTATTAGGGAGCAGCAGATTGGTGGCATTCTTCAGGTCGATTGCATATTAATTAATTAGTTTTCAGCTTTATGGGTTCCATTTGATATTGCTGAATGTTGTTGGGTTTGAATGGTTTCAAATTTTGTTTGTTTTCCCTTTTGTGTGAGAATGTTAGAATGTTGATTGTAGAATGTAGGAGTTACTTTATGTGGGTATGGTGTGTAGATGAAGGTCATCGATGTTGGCGGGATTTTCCTAATTCCCCAATCACTTGTGAACCAATAAACGGAGATTTTAACATGAAAGTCGTACTTTTTGGGCTTAATTTAACAAATTAGATAGTGAATTGTGAACCTGTATCCACTGTCTGGGACTCAAACTAGCGAGTTAGGTAACACTAATCTTATGTGACATACATTTGTTCTTAGATGATGAAACTATGACTTAACTAGAAAGTTTAGCAGGAGAACTCTAGGATAAGTTTCAGCTTTCACTGGTTTTTGAATCTAGCTTTAGGTGTCTGGGAGAAAGAAGTCGAAGCTACAAATGCCGCTAAGAATATATTATTGGATAATGTGCTAGAAATCAGAGGATGTTTGGTTCTAATTGTTAACTACATGGAACTTTTTTTGTTTTTAGCATTGTTAAGATTTGTGTTTTCAAAAATGGAGATTACTTGTCCAATCATGATAGAATTTGATGAAACAAGAACTAAATTCTCAAGCTCAGTTATTTCAATGAATGTATGATTCTTTGCTGATTAAACTACCTTCACTCCTTCAGTTTTGATGTTCAAAGTGAATGCCCATATATATATATATATATATAGCTTACATTCCAATATTCCATGTATATGCTTTTTAATGATTGTTTTATATTTACTAGGCCACCTTTATATCAATCTACTTTTGCCAAAGGTGTGATAGTTGTCTTTTGACAAATGTTAAGGACCACAGTATGATCTAACTATGAAGGGAATACGAAAATACTTCTTTCTCAATACAGTTGTTTCTTTAGGAGGTTGTCCGTAGATGTATTTTCTGTCCTCTTGAAATGCAAGGCAAAGAAATGACCTCTTTCCCGGTTTGCATAGAGAAAAAAATCTGGTATATGAGAGGCGAGGAAAATCATAAGGATTTTTATAATTATATTTGGAAAGTTATGGTGTTCTAGGTTTGTTTCATCATACTGTGTCTTAGAAGAATACATAACAGAATAAGTACTGTAATATTGTATAAGCATTTTTCTCCTCCCTAGCTTTGTTTCTAACACCAAAATGCTTTTAGGTTGGTGCTCATGGCACTGGTGCAAGATTGCCACCTATTGATGAGCAAGTTATTTCCGTGAAACTGGTCACTCCTGCCAAGGGAACTATAGAGCTATCTAAAGAGAAAGATCCGGAGCTATTTTATCTTGCTCGTGTTGGTCTTGGAGGACTCGGAGTAGTGGCTGAAGTCACCCTTCAGTGTGTTGATAGGCAGGAGCTTGTAGAGCAC

mRNA sequence

ATGCACCGAGTAACCAATCTCCGACGATTTATTCCCTCTCTCCTCCACCACCATCACCACCGCCATTTACACCAAAACACCAACCCGATCAAATCCCTCACCTCCGCAAACTCAACCATCCTCCAAAACCCCGCCTTACACCCCCGCTCATTCTCTTCTCTCTCCTCTGACCCTACGGCCTCCACCACCGCCGAAATCCGCAAATACATCGGATACTCCCTCCTCCTCCTCGGTTGCGGCGTTGGAACCTACTATTCCTTCCCTTTCCCGGAAAACGCCAAGCACAAAAAAGCCCAACTCTTCAAATACGCCCCTCTCCCCGAAGATCTTCATACCATCTCTAATTGGAGTGGTACCCATGAGGTCCATACCCGCAATTTCATTCAACCGGAATCGGTTTCGGACCTCGAAAAACTTGTCCAAGAAGCCCATCAAAAGTCCCAGAAGATTCGGCCTGTTGGGTCGGGTCTTTCTCCAAATGGGGTTGGGCTTTCTCGGGCTGGCATGGTGAATTTGGGCTTGTTGGATAAGGTATTGGAGGTTGATAAGGAGAAGAAGACTGTTAGAGTTGAAGCTGGTATTAGAGTTCAGCAGCTTGTTGATCAAATTAAGGAGTACGGACTTACTTTGCAAAATTTTGCTTCTATTAGGGAGCAGCAGATTGGTGGCATTCTTCAGGTTGGTGCTCATGGCACTGGTGCAAGATTGCCACCTATTGATGAGCAAGTTATTTCCGTGAAACTGGTCACTCCTGCCAAGGGAACTATAGAGCTATCTAAAGAGAAAGATCCGGAGCTATTTTATCTTGCTCGTGTTGGTCTTGGAGGACTCGGAGTAGTGGCTGAAGTCACCCTTCAGTGTGTTGATAGGCAGGAGCTTGTAGAGCAC

Coding sequence (CDS)

ATGCACCGAGTAACCAATCTCCGACGATTTATTCCCTCTCTCCTCCACCACCATCACCACCGCCATTTACACCAAAACACCAACCCGATCAAATCCCTCACCTCCGCAAACTCAACCATCCTCCAAAACCCCGCCTTACACCCCCGCTCATTCTCTTCTCTCTCCTCTGACCCTACGGCCTCCACCACCGCCGAAATCCGCAAATACATCGGATACTCCCTCCTCCTCCTCGGTTGCGGCGTTGGAACCTACTATTCCTTCCCTTTCCCGGAAAACGCCAAGCACAAAAAAGCCCAACTCTTCAAATACGCCCCTCTCCCCGAAGATCTTCATACCATCTCTAATTGGAGTGGTACCCATGAGGTCCATACCCGCAATTTCATTCAACCGGAATCGGTTTCGGACCTCGAAAAACTTGTCCAAGAAGCCCATCAAAAGTCCCAGAAGATTCGGCCTGTTGGGTCGGGTCTTTCTCCAAATGGGGTTGGGCTTTCTCGGGCTGGCATGGTGAATTTGGGCTTGTTGGATAAGGTATTGGAGGTTGATAAGGAGAAGAAGACTGTTAGAGTTGAAGCTGGTATTAGAGTTCAGCAGCTTGTTGATCAAATTAAGGAGTACGGACTTACTTTGCAAAATTTTGCTTCTATTAGGGAGCAGCAGATTGGTGGCATTCTTCAGGTTGGTGCTCATGGCACTGGTGCAAGATTGCCACCTATTGATGAGCAAGTTATTTCCGTGAAACTGGTCACTCCTGCCAAGGGAACTATAGAGCTATCTAAAGAGAAAGATCCGGAGCTATTTTATCTTGCTCGTGTTGGTCTTGGAGGACTCGGAGTAGTGGCTGAAGTCACCCTTCAGTGTGTTGATAGGCAGGAGCTTGTAGAGCAC

Protein sequence

MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTHEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPIDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo27157	Spo27157	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo27157.1	Spo27157.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo27157.1.CDS.2	Spo27157.1.CDS.2	CDS
Spo27157.1.CDS.1	Spo27157.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo27157.1.exon.2	Spo27157.1.exon.2	exon
Spo27157.1.exon.1	Spo27157.1.exon.1	exon

Homology

[Close]

BLAST of Spo27157.1 vs. NCBI nr
Match: gi|902150727|gb|KNA05447.1| (hypothetical protein SOVF_190280 [Spinacia oleracea])

HSP 1 Score: 582.4 bits (1500), Expect = 4.400e-163
Identity = 293/296 (98.99%), Postives = 296/296 (100.00%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTA 60
           MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPA++PRSFSSLSSDPTA
Sbjct: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPAINPRSFSSLSSDPTA 60

Query: 61  STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120
           STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH
Sbjct: 61  STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120

Query: 121 EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180
           EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE
Sbjct: 121 EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180

Query: 181 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID 240
           VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID
Sbjct: 181 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID 240

Query: 241 EQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           EQVIS+KLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH
Sbjct: 241 EQVISMKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 296

[Close]

BLAST of Spo27157.1 vs. NCBI nr
Match: gi|731324445|ref|XP_010672973.1| (PREDICTED: L-galactono-1,4-lactone dehydrogenase, mitochondrial [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 474.6 bits (1220), Expect = 1.300e-130
Identity = 243/296 (82.09%), Postives = 259/296 (87.50%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTA 60
           M+R T+ RR I  L     HRH HQ  NP            QNP L+PR FSSLSSDP +
Sbjct: 1   MYRATHFRRSISLL-----HRHYHQTPNP------------QNPFLNPRQFSSLSSDPPS 60

Query: 61  STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120
           S+++E RKYIGY LLLLGCG GTYYSFP PENAKHKKAQLFKYAPLPEDLHTISNWSGTH
Sbjct: 61  SSSSETRKYIGYFLLLLGCGAGTYYSFPLPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120

Query: 121 EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180
           EVHTRN+ QPES+ +LE+LV+EAHQK  KIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE
Sbjct: 121 EVHTRNYFQPESLPELERLVKEAHQKGIKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180

Query: 181 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID 240
           VDKEKK VRV+AGIRVQQLVDQIKE+GLTLQNFASIREQQ+GGILQVGAHGTGARLPPID
Sbjct: 181 VDKEKKIVRVQAGIRVQQLVDQIKEFGLTLQNFASIREQQVGGILQVGAHGTGARLPPID 240

Query: 241 EQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           EQVIS+KLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH
Sbjct: 241 EQVISMKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 279

[Close]

BLAST of Spo27157.1 vs. NCBI nr
Match: gi|559162935|gb|AHB11182.1| (L-galactono-1,4-lactone dehydrogenase [Camellia sinensis])

HSP 1 Score: 433.3 bits (1113), Expect = 3.300e-118
Identity = 221/303 (72.94%), Postives = 253/303 (83.50%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQN-------TNPIKSLTSANSTILQNPALHPRSFSS 60
           M R  N+RR +   LHHHHH H H +       + P+K+L+S      QNP L     SS
Sbjct: 1   MFRALNIRRSLYQALHHHHHHHHHHHHHHHHPLSQPLKTLSST-----QNPNLTRPFCSS 60

Query: 61  LSSDPTASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTI 120
            SS   +S+ AE RKY+GY  LL+GCG+ TYYSFPFPENAKHKKAQLF+YAPLP+DLHT+
Sbjct: 61  TSS---SSSEAEFRKYVGYFALLVGCGIATYYSFPFPENAKHKKAQLFRYAPLPDDLHTV 120

Query: 121 SNWSGTHEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLG 180
           SNWSGTHEV TR F+QPES+ +LE +V++A+ + QKIRPVGSGLSPNG+GL+R GMVNL 
Sbjct: 121 SNWSGTHEVQTRVFLQPESIQELETIVRDANVQKQKIRPVGSGLSPNGIGLTRLGMVNLA 180

Query: 181 LLDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTG 240
           LLD+VLEVDKEKKTVRVEAGIRVQQLVD IK+YGLTLQNFASIREQQIGGI+QVGAHGTG
Sbjct: 181 LLDRVLEVDKEKKTVRVEAGIRVQQLVDGIKDYGLTLQNFASIREQQIGGIVQVGAHGTG 240

Query: 241 ARLPPIDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQEL 297
           ARLPPIDEQV+S+KLVTPAKGTIE+SKEKDPELFYLAR GLG LGVVAEVTLQCV+RQEL
Sbjct: 241 ARLPPIDEQVVSMKLVTPAKGTIEVSKEKDPELFYLARCGLGALGVVAEVTLQCVERQEL 295

[Close]

BLAST of Spo27157.1 vs. NCBI nr
Match: gi|224589061|gb|ACN59217.1| (putative L-galactono-1,4-lactone dehydrogenase [Vitis vinifera])

HSP 1 Score: 432.2 bits (1110), Expect = 7.400e-118
Identity = 222/298 (74.50%), Postives = 254/298 (85.23%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSA-NSTILQNPALH-PRSFSSLSSDP 60
           M R  +LRR + +  H+HHH H  Q TNP  +L  A +ST   NP  +  R FSSLSS P
Sbjct: 1   MLRALSLRRSLQAFHHYHHHHHQQQPTNPHSNLLKALSSTTSPNPNPNLTRPFSSLSSSP 60

Query: 61  TASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSG 120
             S+ AE RKY+GY  LLLGCG+ TYYSFPF ++AKHKKAQLF+YAPLP+DLHT+SNWSG
Sbjct: 61  --SSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFRYAPLPDDLHTVSNWSG 120

Query: 121 THEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKV 180
           THEV TR F QPES+ +LE++V+EA++K QKIRPVGSGLSPNG+GL+RAGMVNL LLD V
Sbjct: 121 THEVQTRVFHQPESLEELEQIVKEANEKKQKIRPVGSGLSPNGIGLTRAGMVNLALLDNV 180

Query: 181 LEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPP 240
           L+VD EKK VRV+AGIRVQQLVD IK+YG+TLQNFASIREQQIGGI+QVGAHGTGARLPP
Sbjct: 181 LDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQVGAHGTGARLPP 240

Query: 241 IDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           IDEQVIS+KLVTPAKGTIE+SKEKDPELFYLAR GLGGLGVVAEVTLQCV+RQELVEH
Sbjct: 241 IDEQVISMKLVTPAKGTIEVSKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEH 296

[Close]

BLAST of Spo27157.1 vs. NCBI nr
Match: gi|359482204|ref|XP_002274214.2| (PREDICTED: L-galactono-1,4-lactone dehydrogenase, mitochondrial [Vitis vinifera])

HSP 1 Score: 426.0 bits (1094), Expect = 5.300e-116
Identity = 220/298 (73.83%), Postives = 254/298 (85.23%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSA-NSTILQNPALH-PRSFSSLSSDP 60
           M R  +LRR + +  H+HHH H  Q TNP  +L  A +ST   NP  +  R FSSLSS P
Sbjct: 1   MLRALSLRRSLRAFHHYHHHHH--QPTNPHSNLLKALSSTTSPNPNPNLTRPFSSLSSSP 60

Query: 61  TASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSG 120
             S+ AE RKY+GY  LLLGCG+ TYYSFPF ++AKHKKAQLF+YAPLP+DLHT+SNWSG
Sbjct: 61  --SSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFRYAPLPDDLHTVSNWSG 120

Query: 121 THEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKV 180
           THEV TR F QPES+ +LE++V+EA++K QKIRPVGSGLSPNG+GL+RAGMVNL L+D V
Sbjct: 121 THEVQTRVFHQPESLEELEQIVKEANEKKQKIRPVGSGLSPNGIGLTRAGMVNLALMDNV 180

Query: 181 LEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPP 240
           L+VD EKK VRV+AGIRVQQLVD IK+YG+TLQNFASIREQQIGGI+QVGAHGTGARLPP
Sbjct: 181 LDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQVGAHGTGARLPP 240

Query: 241 IDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           IDEQVIS+KLVTPAKGTIE+SKE+DPELFYLAR GLGGLGVVAEVTLQCV+RQELVEH
Sbjct: 241 IDEQVISMKLVTPAKGTIEVSKERDPELFYLARCGLGGLGVVAEVTLQCVERQELVEH 294

[Close]

BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QDV4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_190280 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 3.100e-163
Identity = 293/296 (98.99%), Postives = 296/296 (100.00%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTA 60
           MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPA++PRSFSSLSSDPTA
Sbjct: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPAINPRSFSSLSSDPTA 60

Query: 61  STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120
           STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH
Sbjct: 61  STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120

Query: 121 EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180
           EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE
Sbjct: 121 EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180

Query: 181 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID 240
           VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID
Sbjct: 181 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID 240

Query: 241 EQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           EQVIS+KLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH
Sbjct: 241 EQVISMKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 296

[Close]

BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CN19_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g062480 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 9.100e-131
Identity = 243/296 (82.09%), Postives = 259/296 (87.50%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTA 60
           M+R T+ RR I  L     HRH HQ  NP            QNP L+PR FSSLSSDP +
Sbjct: 1   MYRATHFRRSISLL-----HRHYHQTPNP------------QNPFLNPRQFSSLSSDPPS 60

Query: 61  STTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120
           S+++E RKYIGY LLLLGCG GTYYSFP PENAKHKKAQLFKYAPLPEDLHTISNWSGTH
Sbjct: 61  SSSSETRKYIGYFLLLLGCGAGTYYSFPLPENAKHKKAQLFKYAPLPEDLHTISNWSGTH 120

Query: 121 EVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180
           EVHTRN+ QPES+ +LE+LV+EAHQK  KIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE
Sbjct: 121 EVHTRNYFQPESLPELERLVKEAHQKGIKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLE 180

Query: 181 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPID 240
           VDKEKK VRV+AGIRVQQLVDQIKE+GLTLQNFASIREQQ+GGILQVGAHGTGARLPPID
Sbjct: 181 VDKEKKIVRVQAGIRVQQLVDQIKEFGLTLQNFASIREQQVGGILQVGAHGTGARLPPID 240

Query: 241 EQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           EQVIS+KLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH
Sbjct: 241 EQVISMKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 279

[Close]

BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
Match: V5Q782_CAMSI (L-galactono-1,4-lactone dehydrogenase OS=Camellia sinensis GN=GalLDH PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.300e-118
Identity = 221/303 (72.94%), Postives = 253/303 (83.50%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQN-------TNPIKSLTSANSTILQNPALHPRSFSS 60
           M R  N+RR +   LHHHHH H H +       + P+K+L+S      QNP L     SS
Sbjct: 1   MFRALNIRRSLYQALHHHHHHHHHHHHHHHHPLSQPLKTLSST-----QNPNLTRPFCSS 60

Query: 61  LSSDPTASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTI 120
            SS   +S+ AE RKY+GY  LL+GCG+ TYYSFPFPENAKHKKAQLF+YAPLP+DLHT+
Sbjct: 61  TSS---SSSEAEFRKYVGYFALLVGCGIATYYSFPFPENAKHKKAQLFRYAPLPDDLHTV 120

Query: 121 SNWSGTHEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLG 180
           SNWSGTHEV TR F+QPES+ +LE +V++A+ + QKIRPVGSGLSPNG+GL+R GMVNL 
Sbjct: 121 SNWSGTHEVQTRVFLQPESIQELETIVRDANVQKQKIRPVGSGLSPNGIGLTRLGMVNLA 180

Query: 181 LLDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTG 240
           LLD+VLEVDKEKKTVRVEAGIRVQQLVD IK+YGLTLQNFASIREQQIGGI+QVGAHGTG
Sbjct: 181 LLDRVLEVDKEKKTVRVEAGIRVQQLVDGIKDYGLTLQNFASIREQQIGGIVQVGAHGTG 240

Query: 241 ARLPPIDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQEL 297
           ARLPPIDEQV+S+KLVTPAKGTIE+SKEKDPELFYLAR GLG LGVVAEVTLQCV+RQEL
Sbjct: 241 ARLPPIDEQVVSMKLVTPAKGTIEVSKEKDPELFYLARCGLGALGVVAEVTLQCVERQEL 295

[Close]

BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
Match: C0LVB5_VITVI (Putative L-galactono-1,4-lactone dehydrogenase (Fragment) OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 5.200e-118
Identity = 222/298 (74.50%), Postives = 254/298 (85.23%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSA-NSTILQNPALH-PRSFSSLSSDP 60
           M R  +LRR + +  H+HHH H  Q TNP  +L  A +ST   NP  +  R FSSLSS P
Sbjct: 1   MLRALSLRRSLQAFHHYHHHHHQQQPTNPHSNLLKALSSTTSPNPNPNLTRPFSSLSSSP 60

Query: 61  TASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSG 120
             S+ AE RKY+GY  LLLGCG+ TYYSFPF ++AKHKKAQLF+YAPLP+DLHT+SNWSG
Sbjct: 61  --SSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFRYAPLPDDLHTVSNWSG 120

Query: 121 THEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKV 180
           THEV TR F QPES+ +LE++V+EA++K QKIRPVGSGLSPNG+GL+RAGMVNL LLD V
Sbjct: 121 THEVQTRVFHQPESLEELEQIVKEANEKKQKIRPVGSGLSPNGIGLTRAGMVNLALLDNV 180

Query: 181 LEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPP 240
           L+VD EKK VRV+AGIRVQQLVD IK+YG+TLQNFASIREQQIGGI+QVGAHGTGARLPP
Sbjct: 181 LDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQVGAHGTGARLPP 240

Query: 241 IDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           IDEQVIS+KLVTPAKGTIE+SKEKDPELFYLAR GLGGLGVVAEVTLQCV+RQELVEH
Sbjct: 241 IDEQVISMKLVTPAKGTIEVSKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEH 296

[Close]

BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
Match: F6HLP5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g05710 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.700e-116
Identity = 220/298 (73.83%), Postives = 254/298 (85.23%), Query Frame = 1


		  
Query: 1   MHRVTNLRRFIPSLLHHHHHRHLHQNTNPIKSLTSA-NSTILQNPALH-PRSFSSLSSDP 60
           M R  +LRR + +  H+HHH H  Q TNP  +L  A +ST   NP  +  R FSSLSS P
Sbjct: 1   MLRALSLRRSLRAFHHYHHHHH--QPTNPHSNLLKALSSTTSPNPNPNLTRPFSSLSSSP 60

Query: 61  TASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSG 120
             S+ AE RKY+GY  LLLGCG+ TYYSFPF ++AKHKKAQLF+YAPLP+DLHT+SNWSG
Sbjct: 61  --SSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFRYAPLPDDLHTVSNWSG 120

Query: 121 THEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKV 180
           THEV TR F QPES+ +LE++V+EA++K QKIRPVGSGLSPNG+GL+RAGMVNL L+D V
Sbjct: 121 THEVQTRVFHQPESLEELEQIVKEANEKKQKIRPVGSGLSPNGIGLTRAGMVNLALMDNV 180

Query: 181 LEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPP 240
           L+VD EKK VRV+AGIRVQQLVD IK+YG+TLQNFASIREQQIGGI+QVGAHGTGARLPP
Sbjct: 181 LDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQVGAHGTGARLPP 240

Query: 241 IDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           IDEQVIS+KLVTPAKGTIE+SKE+DPELFYLAR GLGGLGVVAEVTLQCV+RQELVEH
Sbjct: 241 IDEQVISMKLVTPAKGTIEVSKERDPELFYLARCGLGGLGVVAEVTLQCVERQELVEH 294

[Close]

BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
Match: GLDH_BRAOL (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 5.700e-110
Identity = 202/283 (71.38%), Postives = 235/283 (83.04%), Query Frame = 1


		  
Query: 14  LLHHHHHRHLHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTASTTAEIRKYIGYS 73
           LL   + R L     P+++L ++  T+   P   P     +SS   +++  E RKY GY+
Sbjct: 6   LLRRSNARSLRPPFPPLRTLCTSGQTLTPAPPPPPPPPPPISS---SASEKEFRKYAGYA 65

Query: 74  LLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTHEVHTRNFIQPESV 133
            L L  G  TY+SFPFPENAKHKKAQ+F+YAPLPEDLHT+SNWSGTHEV TRNF QPE++
Sbjct: 66  ALALFSGAATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQPETL 125

Query: 134 SDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLEVDKEKKTVRVEAG 193
           +DLE LV+EAH+K  +IRPVGSGLSPNG+GLSR+GMVNL L+DKVLEVDKEKK VRV+AG
Sbjct: 126 ADLEALVKEAHEKKNRIRPVGSGLSPNGIGLSRSGMVNLALMDKVLEVDKEKKRVRVQAG 185

Query: 194 IRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPIDEQVISVKLVTPAK 253
           IRVQQLVD I+EYGLTLQNFASIREQQIGGI+QVGAHGTGARLPPIDEQVI +KLVTPAK
Sbjct: 186 IRVQQLVDAIQEYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPIDEQVIGMKLVTPAK 245

Query: 254 GTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           GTIELSK+ DPELF+LAR GLGGLGVVAEVTLQCV+RQEL+EH
Sbjct: 246 GTIELSKDNDPELFHLARCGLGGLGVVAEVTLQCVERQELLEH 285

[Close]

BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
Match: GLDH_ARATH (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana GN=GLDH PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 7.400e-110
Identity = 198/274 (72.26%), Postives = 230/274 (83.94%), Query Frame = 1


		  
Query: 23  LHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTASTTAEIRKYIGYSLLLLGCGVG 82
           +  + +P ++L +   T+   P   PR        P  ++ A+ RKY GY+ L +  GV 
Sbjct: 25  IRSSFSPHRTLCTTGQTLTPPPPPPPRP---PPPPPATASEAQFRKYAGYAALAIFSGVA 84

Query: 83  TYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTHEVHTRNFIQPESVSDLEKLVQE 142
           TY+SFPFPENAKHKKAQ+F+YAPLPEDLHT+SNWSGTHEV TRNF QPE+++DLE LV+E
Sbjct: 85  TYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKE 144

Query: 143 AHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLEVDKEKKTVRVEAGIRVQQLVDQ 202
           +H+K  +IRPVGSGLSPNG+GLSR+GMVNL L+DKVLEVDKEKK V V+AGIRVQQLVD 
Sbjct: 145 SHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDA 204

Query: 203 IKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPIDEQVISVKLVTPAKGTIELSKEK 262
           IK+YGLTLQNFASIREQQIGGI+QVGAHGTGARLPPIDEQVIS+KLVTPAKGTIELS+EK
Sbjct: 205 IKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREK 264

Query: 263 DPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           DPELF+LAR GLGGLGVVAEVTLQCV R ELVEH
Sbjct: 265 DPELFHLARCGLGGLGVVAEVTLQCVARHELVEH 295

[Close]

BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
Match: GLDH2_ORYSJ (L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica GN=GLDH2 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.400e-100
Identity = 175/242 (72.31%), Postives = 209/242 (86.36%), Query Frame = 1


		  
Query: 55  SSDPTASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTIS 114
           +S P  ++ A++RKY GY+LLLLGCG  TYYSFP P +A HKKA  FKYAPLP+DLH +S
Sbjct: 31  ASSPLPASDADLRKYAGYALLLLGCGAATYYSFPLPPDALHKKAVPFKYAPLPDDLHAVS 90

Query: 115 NWSGTHEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGL 174
           NWS THEVHTR  +QP+S+  L   +  AH + +K+RP+GSGLSPNG+ LSRAGMVNL L
Sbjct: 91  NWSATHEVHTRVLLQPDSLPVLHDALAAAHGERRKLRPLGSGLSPNGLALSRAGMVNLAL 150

Query: 175 LDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGA 234
           +DKVL+VD +KKTV V+AGIRV +LVD ++E+GLTLQNFASIREQQ+GGI+QVGAHGTGA
Sbjct: 151 MDKVLDVDAKKKTVTVQAGIRVAELVDTLREHGLTLQNFASIREQQVGGIIQVGAHGTGA 210

Query: 235 RLPPIDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELV 294
           RLPPIDEQVIS+KLVTPAKGTIELS+EKDP+LFYLAR GLGGLGVVAEVTLQCV+R +L+
Sbjct: 211 RLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYLARCGLGGLGVVAEVTLQCVERHQLI 270

Query: 295 EH 297
           EH
Sbjct: 271 EH 272

[Close]

BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
Match: GLDH1_ORYSJ (L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica GN=GLDH1 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.100e-100
Identity = 175/242 (72.31%), Postives = 208/242 (85.95%), Query Frame = 1


		  
Query: 55  SSDPTASTTAEIRKYIGYSLLLLGCGVGTYYSFPFPENAKHKKAQLFKYAPLPEDLHTIS 114
           +S P  ++ A++RKY GY+LLLLGCG  TYYSFP P +A HKKA  FKYAPLP+DLH +S
Sbjct: 31  ASSPLPASDADLRKYAGYALLLLGCGAATYYSFPLPPDALHKKAVPFKYAPLPDDLHAVS 90

Query: 115 NWSGTHEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGL 174
           NWS THEVHTR  +QP+S+  L   +  AH + +K+RP+GSGLSPNG+ LSRAGMVNL L
Sbjct: 91  NWSATHEVHTRVLLQPDSLPALHDALAAAHGECRKLRPLGSGLSPNGLALSRAGMVNLAL 150

Query: 175 LDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGA 234
           +DKVL VD +KKTV V+AGIRV +LVD ++E+GLTLQNFASIREQQ+GGI+QVGAHGTGA
Sbjct: 151 MDKVLGVDAKKKTVTVQAGIRVAELVDALREHGLTLQNFASIREQQVGGIIQVGAHGTGA 210

Query: 235 RLPPIDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELV 294
           RLPPIDEQVIS+KLVTPAKGTIELS+EKDP+LFYLAR GLGGLGVVAEVTLQCV+R +L+
Sbjct: 211 RLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYLARCGLGGLGVVAEVTLQCVERHQLI 270

Query: 295 EH 297
           EH
Sbjct: 271 EH 272

[Close]

BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
Match: GGLO_MOUSE (L-gulonolactone oxidase OS=Mus musculus GN=Gulo PE=1 SV=3)

HSP 1 Score: 131.3 bits (329), Expect = 1.700e-29
Identity = 65/181 (35.91%), Postives = 112/181 (61.88%), Query Frame = 1


		  
Query: 115 NWSGTHEVHTRNFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGL 174
           NW+ T+      + QP SV ++ +++  A Q+++K++ VG G SP+ +  +   M+++G 
Sbjct: 12  NWAKTYGCSPEMYYQPTSVGEVREVLALARQQNKKVKVVGGGHSPSDIACTDGFMIHMGK 71

Query: 175 LDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTGA 234
           +++VL+VDKEKK V VEAGI +  L  Q+ ++GL L N  ++ +  +GG++  G H TG 
Sbjct: 72  MNRVLQVDKEKKQVTVEAGILLTDLHPQLDKHGLALSNLGAVSDVTVGGVIGSGTHNTGI 131

Query: 235 RLPPIDEQVISVKLVTPAKGTIELSKEKDPELFYLARVGLGGLGVVAEVTLQCVDRQELV 294
           +   +  QV+++ L+      +E S+  + ++F  ARV LG LGV+  VTLQCV +  L+
Sbjct: 132 KHGILATQVVALTLMKADGTVLECSESSNADVFQAARVHLGCLGVILTVTLQCVPQFHLL 191

Query: 295 E 296
           E
Sbjct: 192 E 192

[Close]

BLAST of Spo27157.1 vs. TAIR (Arabidopsis)
Match: AT3G47930.1 (L-galactono-1,4-lactone dehydrogenase)

HSP 1 Score: 398.3 bits (1022), Expect = 4.200e-111
Identity = 198/274 (72.26%), Postives = 230/274 (83.94%), Query Frame = 1


		  
Query: 23  LHQNTNPIKSLTSANSTILQNPALHPRSFSSLSSDPTASTTAEIRKYIGYSLLLLGCGVG 82
           +  + +P ++L +   T+   P   PR        P  ++ A+ RKY GY+ L +  GV 
Sbjct: 25  IRSSFSPHRTLCTTGQTLTPPPPPPPRP---PPPPPATASEAQFRKYAGYAALAIFSGVA 84

Query: 83  TYYSFPFPENAKHKKAQLFKYAPLPEDLHTISNWSGTHEVHTRNFIQPESVSDLEKLVQE 142
           TY+SFPFPENAKHKKAQ+F+YAPLPEDLHT+SNWSGTHEV TRNF QPE+++DLE LV+E
Sbjct: 85  TYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKE 144

Query: 143 AHQKSQKIRPVGSGLSPNGVGLSRAGMVNLGLLDKVLEVDKEKKTVRVEAGIRVQQLVDQ 202
           +H+K  +IRPVGSGLSPNG+GLSR+GMVNL L+DKVLEVDKEKK V V+AGIRVQQLVD 
Sbjct: 145 SHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDA 204

Query: 203 IKEYGLTLQNFASIREQQIGGILQVGAHGTGARLPPIDEQVISVKLVTPAKGTIELSKEK 262
           IK+YGLTLQNFASIREQQIGGI+QVGAHGTGARLPPIDEQVIS+KLVTPAKGTIELS+EK
Sbjct: 205 IKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREK 264

Query: 263 DPELFYLARVGLGGLGVVAEVTLQCVDRQELVEH 297
           DPELF+LAR GLGGLGVVAEVTLQCV R ELVEH
Sbjct: 265 DPELFHLARCGLGGLGVVAEVTLQCVARHELVEH 295

[Close]

BLAST of Spo27157.1 vs. TAIR (Arabidopsis)
Match: AT1G32300.1 (D-arabinono-1,4-lactone oxidase family protein)

HSP 1 Score: 65.5 bits (158), Expect = 6.400e-11
Identity = 47/169 (27.81%), Postives = 90/169 (53.25%), Query Frame = 1


		  
Query: 130 PESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGLSRAG----MVNLGLLDKVLEVDKEK 189
           P++ ++L  +V  A +  QK+R V   +      +   G    +++   L+ V+  + E 
Sbjct: 57  PKTEAELVSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEA 116

Query: 190 KTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTG--ARLPPIDEQV 249
           KT+ VE+G+ ++QL+ +  E  L L +        +GG++  GAHG+    +   + + V
Sbjct: 117 KTLTVESGVTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYV 176

Query: 250 ISVKLVTP---AKGTIE---LSKEKDPELFYLARVGLGGLGVVAEVTLQ 287
             +++V+P   + G ++   LS+  DP+ F  A+V LG LGV+++VT Q
Sbjct: 177 SEIRMVSPGLASDGYVKVRVLSETIDPDEFRAAKVSLGVLGVISQVTFQ 225

[Close]

BLAST of Spo27157.1 vs. TAIR (Arabidopsis)
Match: AT5G56490.1 (D-arabinono-1,4-lactone oxidase family protein)

HSP 1 Score: 65.1 bits (157), Expect = 8.400e-11
Identity = 49/169 (28.99%), Postives = 90/169 (53.25%), Query Frame = 1


		  
Query: 130 PESVSDLEKLVQEAHQKSQKIRPVG--SGLSPNGVGLSRAG--MVNLGLLDKVLEVDKEK 189
           P + ++L  +V  A +  QK+R V   S   P  V        +++   L+ V+  + E 
Sbjct: 56  PRTEAELVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEA 115

Query: 190 KTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTG--ARLPPIDEQV 249
           KT+ VE+G+ ++QL+++  ++ L L          +GG++  GAHG+    +   + + V
Sbjct: 116 KTLTVESGVTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYV 175

Query: 250 ISVKLVTP---AKGTIE---LSKEKDPELFYLARVGLGGLGVVAEVTLQ 287
             ++LV+P   + G ++   LS+  DPE F  A+V LG LGV+++VT +
Sbjct: 176 TEIRLVSPGLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFE 224

[Close]

BLAST of Spo27157.1 vs. TAIR (Arabidopsis)
Match: AT2G46740.1 (D-arabinono-1,4-lactone oxidase family protein)

HSP 1 Score: 55.5 bits (132), Expect = 6.700e-8
Identity = 34/127 (26.77%), Postives = 67/127 (52.76%), Query Frame = 1


		  
Query: 170 VNLGLLDKVLEVDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGA 229
           ++   L+  ++ D +  T+ VE+G+ ++QL+ +  + GL L          +GG++  GA
Sbjct: 114 ISTKFLNHTVQADAKAMTLTVESGVTLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGA 173

Query: 230 HGTG--ARLPPIDEQVISVKLVTPAKGTIE--------LSKEKDPELFYLARVGLGGLGV 287
           HG+    +   + + V  +++V+P  G++         LS+   P  F  A+V LG LGV
Sbjct: 174 HGSSLWGKGSAVHDYVTEIRMVSP--GSVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGV 233

[Close]

BLAST of Spo27157.1 vs. TAIR (Arabidopsis)
Match: AT2G46750.1 (D-arabinono-1,4-lactone oxidase family protein)

HSP 1 Score: 55.1 bits (131), Expect = 8.700e-8
Identity = 45/176 (25.57%), Postives = 87/176 (49.43%), Query Frame = 1


		  
Query: 126 NFIQPESVSDLEKLVQEAHQKSQKIRPVGSGLSPNGVGL-----SRAGMVNLGLLDKVLE 185
           N   P++ ++L  +V  A Q  +K+R V +  S +   L     +    ++   L+  ++
Sbjct: 62  NVAYPKTEAELVSVVAAATQAGRKMR-VTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQ 121

Query: 186 VDKEKKTVRVEAGIRVQQLVDQIKEYGLTLQNFASIREQQIGGILQVGAHGTG--ARLPP 245
            D    T+ VE+G+ ++QL+ +  + GL L          +GG++  GAHG+    +   
Sbjct: 122 ADATAMTMTVESGMTLRQLIVEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSA 181

Query: 246 IDEQVISVKLVTPAKGTIE--------LSKEKDPELFYLARVGLGGLGVVAEVTLQ 287
           + + V  +++V+P  G++         LS+   P  F  A+V LG LGV+++VT +
Sbjct: 182 VHDYVTEIRMVSP--GSVNEGFAKIRILSETTTPNEFNAAKVSLGVLGVISQVTFE 234

The following BLAST results are available for this feature:

BLAST of Spo27157.1 vs. NCBI nr
BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
BLAST of Spo27157.1 vs. TAIR (Arabidopsis)

BLAST of Spo27157.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902150727\|gb\|KNA05447.1\|	4.4e-163	98.9	hypothetical protein SOVF_1902...	[more]
gi\|731324445\|ref\|XP_010672973.1\|	1.3e-130	82.0	PREDICTED: L-galactono-1,4-lac...	[more]
gi\|559162935\|gb\|AHB11182.1\|	3.3e-118	72.9	L-galactono-1,4-lactone dehydr...	[more]
gi\|224589061\|gb\|ACN59217.1\|	7.4e-118	74.5	putative L-galactono-1,4-lacto...	[more]
gi\|359482204\|ref\|XP_002274214.2\|	5.3e-116	73.8	PREDICTED: L-galactono-1,4-lac...	[more]

BLAST of Spo27157.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QDV4_SPIOL	3.1e-163	98.9	Uncharacterized protein OS=Spi...	[more]
A0A0J8CN19_BETVU	9.1e-131	82.0	Uncharacterized protein OS=Bet...	[more]
V5Q782_CAMSI	2.3e-118	72.9	L-galactono-1,4-lactone dehydr...	[more]
C0LVB5_VITVI	5.2e-118	74.5	Putative L-galactono-1,4-lacto...	[more]
F6HLP5_VITVI	3.7e-116	73.8	Putative uncharacterized prote...	[more]

BLAST of Spo27157.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
GLDH_BRAOL	5.7e-110	71.3	L-galactono-1,4-lactone dehydr...	[more]
GLDH_ARATH	7.4e-110	72.2	L-galactono-1,4-lactone dehydr...	[more]
GLDH2_ORYSJ	1.4e-100	72.3	L-galactono-1,4-lactone dehydr...	[more]
GLDH1_ORYSJ	3.1e-100	72.3	L-galactono-1,4-lactone dehydr...	[more]
GGLO_MOUSE	1.7e-29	35.9	L-gulonolactone oxidase OS=Mus...	[more]

BLAST of Spo27157.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G47930.1	4.2e-111	72.2	L-galactono-1,4-lactone dehydr...	[more]
AT1G32300.1	6.4e-11	27.8	D-arabinono-1,4-lactone oxidas...	[more]
AT5G56490.1	8.4e-11	28.9	D-arabinono-1,4-lactone oxidas...	[more]
AT2G46740.1	6.7e-8	26.7	D-arabinono-1,4-lactone oxidas...	[more]
AT2G46750.1	8.7e-8	25.5	D-arabinono-1,4-lactone oxidas...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 127..256 score: 1.1
IPR010029	Galactonolactone dehydrogenase	TIGRFAMs	TIGR01676	TIGR01676	coord: 64..296 score: 3.6E
IPR016166	FAD-binding, type 2	PROFILE	PS51387	FAD_PCMH	coord: 120..290 score: 17
IPR016166	FAD-binding, type 2	unknown	SSF56176	FAD-binding/transporter-associated domain-like	coord: 107..288 score: 7.01
IPR016167	FAD-binding, type 2, subdomain 1	GENE3D	3.30.43.10		coord: 112..180 score: 2.0
IPR016169	CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2	GENE3D	3.30.465.10		coord: 181..288 score: 4.2
None	No IPR available	PANTHER	PTHR13878	GULONOLACTONE OXIDASE	coord: 24..296 score: 4.4E
None	No IPR available	PANTHER	PTHR13878:SF37	L-GALACTONO-1,4-LACTONE DEHYDROGENASE, MITOCHONDRIAL	coord: 24..296 score: 4.4E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006040	amino sugar metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019852	L-ascorbic acid metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016633	galactonolactone dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors
molecular_function	GO:0003885	D-arabinono-1,4-lactone oxidase activity
molecular_function	GO:0008762	UDP-N-acetylmuramate dehydrogenase activity