Spo28091.1 (mRNA) | SpinachBase

Overview


Name	Spo28091.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	RING finger family protein
Location	chr6 : 35917627 .. 35918118 (+)
Sequence length	492

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCCATACTTCCTCATTAACACTCTATCCCTCCTTAGTTTCTCAAGACCTTTCATCCTTACACTCTTAAGACTCTTGGGGCTCTCCCACATTCTCGAATCCTCGATCAACTGGCCCGAACCGGAACCGGAATCCAAGCCCGCATCGGCCTCCATAATCCAAGAGATGCTGCCTGTTATGAAGTTCACCGACCTCGAATGTGGCACCACACCAGAACAGTGTGCCGTTTGCTTGAACGAGTTTAAAAGTCATGAGGAAATCAGAGTGCTAACAAACTGCAACCATGTATTTCACAGGGCCTGCGTTGACAGTTGGATGAACCATGATCAGATCAGTTGCCCGTTATGCAGGACACACATCGTCCCTAATGAGCTTTTAGAGGTTTTCCATGAAAAGGTTGAAGCTTTAGCTGCAATTTCTGGTGTTCTTGAATTTGGTGGGGAGTTTTCTGTAATTAATAGTTTCTTCTTAGAATGTCCTCTTAGATAA

mRNA sequence

ATGCTCCCATACTTCCTCATTAACACTCTATCCCTCCTTAGTTTCTCAAGACCTTTCATCCTTACACTCTTAAGACTCTTGGGGCTCTCCCACATTCTCGAATCCTCGATCAACTGGCCCGAACCGGAACCGGAATCCAAGCCCGCATCGGCCTCCATAATCCAAGAGATGCTGCCTGTTATGAAGTTCACCGACCTCGAATGTGGCACCACACCAGAACAGTGTGCCGTTTGCTTGAACGAGTTTAAAAGTCATGAGGAAATCAGAGTGCTAACAAACTGCAACCATGTATTTCACAGGGCCTGCGTTGACAGTTGGATGAACCATGATCAGATCAGTTGCCCGTTATGCAGGACACACATCGTCCCTAATGAGCTTTTAGAGGTTTTCCATGAAAAGGTTGAAGCTTTAGCTGCAATTTCTGGTGTTCTTGAATTTGGTGGGGAGTTTTCTGTAATTAATAGTTTCTTCTTAGAATGTCCTCTTAGATAA

Coding sequence (CDS)

ATGCTCCCATACTTCCTCATTAACACTCTATCCCTCCTTAGTTTCTCAAGACCTTTCATCCTTACACTCTTAAGACTCTTGGGGCTCTCCCACATTCTCGAATCCTCGATCAACTGGCCCGAACCGGAACCGGAATCCAAGCCCGCATCGGCCTCCATAATCCAAGAGATGCTGCCTGTTATGAAGTTCACCGACCTCGAATGTGGCACCACACCAGAACAGTGTGCCGTTTGCTTGAACGAGTTTAAAAGTCATGAGGAAATCAGAGTGCTAACAAACTGCAACCATGTATTTCACAGGGCCTGCGTTGACAGTTGGATGAACCATGATCAGATCAGTTGCCCGTTATGCAGGACACACATCGTCCCTAATGAGCTTTTAGAGGTTTTCCATGAAAAGGTTGAAGCTTTAGCTGCAATTTCTGGTGTTCTTGAATTTGGTGGGGAGTTTTCTGTAATTAATAGTTTCTTCTTAGAATGTCCTCTTAGATAA

Protein sequence

MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEPESKPASASIIQEMLPVMKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo28091	Spo28091	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo28091.1	Spo28091.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo28091.1.exon.1	Spo28091.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo28091.1.CDS.1	Spo28091.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo28091.1 vs. NCBI nr
Match: gi|902239639|gb|KNA25665.1| (hypothetical protein SOVF_004480 [Spinacia oleracea])

HSP 1 Score: 332.8 bits (852), Expect = 3.400e-88
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEPESKPASASIIQEMLPV 60
           MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEP SKPASASIIQEMLPV
Sbjct: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEPVSKPASASIIQEMLPV 60

Query: 61  MKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTH 120
           MKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTH
Sbjct: 61  MKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTH 120

Query: 121 IVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR 164
           IVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR
Sbjct: 121 IVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR 163

[Close]

BLAST of Spo28091.1 vs. NCBI nr
Match: gi|731331031|ref|XP_010676446.1| (PREDICTED: E3 ubiquitin-protein ligase RHA1B [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 223.8 bits (569), Expect = 2.200e-55
Identity = 110/158 (69.62%), Postives = 126/158 (79.75%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEPESKPASASIIQEMLPV 60
           MLP    NTLSLL+F R FIL  LRLLGLSHILE  I WPEPEPE +PASASIIQEMLP+
Sbjct: 11  MLPKLFTNTLSLLNFLRDFILASLRLLGLSHILEPQIFWPEPEPEFRPASASIIQEMLPI 70

Query: 61  MKFTDLECG--TTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCR 120
           MKF D+  G  T PEQCAVCL EF+ HEEIR L NC H+FHR C+D WM+HDQI+CPLCR
Sbjct: 71  MKFADVVHGPDTGPEQCAVCLYEFRDHEEIRRLVNCMHIFHRGCLDRWMDHDQITCPLCR 130

Query: 121 THIVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSF 157
           T IVP+EL+E FH+K+ A  AIS + EFGGEFS+IN+F
Sbjct: 131 TLIVPDELMETFHKKLGAATAISTIPEFGGEFSIINNF 168

[Close]

BLAST of Spo28091.1 vs. NCBI nr
Match: gi|703162027|ref|XP_010112947.1| (E3 ubiquitin-protein ligase RHA1B [Morus notabilis])

HSP 1 Score: 157.9 bits (398), Expect = 1.500e-35
Identity = 80/159 (50.31%), Postives = 106/159 (66.67%), Query Frame = 1


		  
Query: 3   PYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPE------PESKPASASIIQE 62
           P   ++TLSLL F R  I +L   LGLS  LE+ + WP+        PE+ P SA +I+E
Sbjct: 13  PNLFLHTLSLLGFIRNLIFSLFHFLGLSDFLETDVVWPQSSSPPAQSPENPPVSALLIRE 72

Query: 63  MLPVMKFTDLE----CGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQI 122
           +LPV+KF+DL+     G +PE CAVCL EF   EEIR LTNC H+FHRAC+D WM+HDQ 
Sbjct: 73  LLPVIKFSDLDSVAGAGNSPESCAVCLYEFDGEEEIRWLTNCRHIFHRACLDRWMDHDQK 132

Query: 123 SCPLCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFS 152
           +CPLCRT  VP+E+ + F+++   L A SGV +F  E+S
Sbjct: 133 TCPLCRTPFVPDEMKDEFNQR---LWAASGVADFYSEYS 168

[Close]

BLAST of Spo28091.1 vs. NCBI nr
Match: gi|567904996|ref|XP_006444986.1| (hypothetical protein CICLE_v10024594mg [Citrus clementina])

HSP 1 Score: 157.1 bits (396), Expect = 2.500e-35
Identity = 82/158 (51.90%), Postives = 106/158 (67.09%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEP----ESKPASASIIQEM 61
           LP F ++TLS L F R  I+ L R LGLS  LE+ + WP+  P    E+ P SA +I+E+
Sbjct: 12  LPKFFVHTLSFLGFIRNIIICLFRYLGLSDFLETDVIWPDNSPTRIPENAPVSARLIREI 71

Query: 62  LPVMKFTDLEC--GTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCP 121
           LPV+KF DLE   G  PE CAVCL EF+  EEIR L NC H+FHRAC+D WM+HDQ +CP
Sbjct: 72  LPVIKFQDLEMVNGDPPENCAVCLYEFEGGEEIRWLRNCKHIFHRACLDPWMDHDQKTCP 131

Query: 122 LCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFSVI 154
           LCRT  VP+E+ E F+++   L A SGV +   E+S +
Sbjct: 132 LCRTPFVPDEMQEEFNQR---LWAASGVDDLFSEYSSV 166

[Close]

BLAST of Spo28091.1 vs. NCBI nr
Match: gi|255537543|ref|XP_002509838.1| (PREDICTED: E3 ubiquitin-protein ligase RHA1B [Ricinus communis])

HSP 1 Score: 156.8 bits (395), Expect = 3.300e-35
Identity = 80/158 (50.63%), Postives = 110/158 (69.62%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPE---PESKPASASIIQEM 60
           +LP  LI+TLS+L F R  I TL R LGL   LE  I+WPE     PE   ASA +I+E+
Sbjct: 11  LLPKLLIHTLSILGFIRKLINTLFRYLGLPDFLEPDISWPETPSRVPEFYSASAVLIREI 70

Query: 61  LPVMKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLC 120
           LPV+KF+DL     P+ CAVCL EF+ H+EIR LTNC H+FH+AC+D W+ +DQ +CPLC
Sbjct: 71  LPVVKFSDLV--DPPDSCAVCLYEFEDHDEIRRLTNCRHIFHKACLDRWVGYDQKTCPLC 130

Query: 121 RTHIVPNELLEVFHEKVEALAAISGVLEFGGEFSVINS 156
           RT ++P+++ E F+E+   L A SG+ +F G++S I +
Sbjct: 131 RTPVIPDDMQEAFNER---LWAASGIPDFYGDYSQIGA 163

[Close]

BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S1P2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_004480 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 2.400e-88
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEPESKPASASIIQEMLPV 60
           MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEP SKPASASIIQEMLPV
Sbjct: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEPVSKPASASIIQEMLPV 60

Query: 61  MKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTH 120
           MKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTH
Sbjct: 61  MKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLCRTH 120

Query: 121 IVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR 164
           IVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR
Sbjct: 121 IVPNELLEVFHEKVEALAAISGVLEFGGEFSVINSFFLECPLR 163

[Close]

BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
Match: W9SMG3_9ROSA (E3 ubiquitin-protein ligase RHA1B OS=Morus notabilis GN=L484_021468 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.000e-35
Identity = 80/159 (50.31%), Postives = 106/159 (66.67%), Query Frame = 1


		  
Query: 3   PYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPE------PESKPASASIIQE 62
           P   ++TLSLL F R  I +L   LGLS  LE+ + WP+        PE+ P SA +I+E
Sbjct: 13  PNLFLHTLSLLGFIRNLIFSLFHFLGLSDFLETDVVWPQSSSPPAQSPENPPVSALLIRE 72

Query: 63  MLPVMKFTDLE----CGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQI 122
           +LPV+KF+DL+     G +PE CAVCL EF   EEIR LTNC H+FHRAC+D WM+HDQ 
Sbjct: 73  LLPVIKFSDLDSVAGAGNSPESCAVCLYEFDGEEEIRWLTNCRHIFHRACLDRWMDHDQK 132

Query: 123 SCPLCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFS 152
           +CPLCRT  VP+E+ + F+++   L A SGV +F  E+S
Sbjct: 133 TCPLCRTPFVPDEMKDEFNQR---LWAASGVADFYSEYS 168

[Close]

BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
Match: A0A067H5S5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g030902mg PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.800e-35
Identity = 82/158 (51.90%), Postives = 106/158 (67.09%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEP----ESKPASASIIQEM 61
           LP F ++TLS L F R  I+ L R LGLS  LE+ + WP+  P    E+ P SA +I+E+
Sbjct: 12  LPKFFVHTLSFLGFIRNIIICLFRYLGLSDFLETDVIWPDNSPTRIPENAPVSARLIREI 71

Query: 62  LPVMKFTDLEC--GTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCP 121
           LPV+KF DLE   G  PE CAVCL EF+  EEIR L NC H+FHRAC+D WM+HDQ +CP
Sbjct: 72  LPVIKFQDLEMVNGDPPENCAVCLYEFEGGEEIRWLRNCKHIFHRACLDPWMDHDQKTCP 131

Query: 122 LCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFSVI 154
           LCRT  VP+E+ E F+++   L A SGV +   E+S +
Sbjct: 132 LCRTPFVPDEMQEEFNQR---LWAASGVDDLFSEYSSV 166

[Close]

BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
Match: V4U7S5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024594mg PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.800e-35
Identity = 82/158 (51.90%), Postives = 106/158 (67.09%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPEP----ESKPASASIIQEM 61
           LP F ++TLS L F R  I+ L R LGLS  LE+ + WP+  P    E+ P SA +I+E+
Sbjct: 12  LPKFFVHTLSFLGFIRNIIICLFRYLGLSDFLETDVIWPDNSPTRIPENAPVSARLIREI 71

Query: 62  LPVMKFTDLEC--GTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCP 121
           LPV+KF DLE   G  PE CAVCL EF+  EEIR L NC H+FHRAC+D WM+HDQ +CP
Sbjct: 72  LPVIKFQDLEMVNGDPPENCAVCLYEFEGGEEIRWLRNCKHIFHRACLDPWMDHDQKTCP 131

Query: 122 LCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFSVI 154
           LCRT  VP+E+ E F+++   L A SGV +   E+S +
Sbjct: 132 LCRTPFVPDEMQEEFNQR---LWAASGVDDLFSEYSSV 166

[Close]

BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
Match: B9RCF4_RICCO (Zinc finger protein, putative OS=Ricinus communis GN=RCOM_1687660 PE=4 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.300e-35
Identity = 80/158 (50.63%), Postives = 110/158 (69.62%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPEPE---PESKPASASIIQEM 60
           +LP  LI+TLS+L F R  I TL R LGL   LE  I+WPE     PE   ASA +I+E+
Sbjct: 11  LLPKLLIHTLSILGFIRKLINTLFRYLGLPDFLEPDISWPETPSRVPEFYSASAVLIREI 70

Query: 61  LPVMKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQISCPLC 120
           LPV+KF+DL     P+ CAVCL EF+ H+EIR LTNC H+FH+AC+D W+ +DQ +CPLC
Sbjct: 71  LPVVKFSDLV--DPPDSCAVCLYEFEDHDEIRRLTNCRHIFHKACLDRWVGYDQKTCPLC 130

Query: 121 RTHIVPNELLEVFHEKVEALAAISGVLEFGGEFSVINS 156
           RT ++P+++ E F+E+   L A SG+ +F G++S I +
Sbjct: 131 RTPVIPDDMQEAFNER---LWAASGIPDFYGDYSQIGA 163

[Close]

BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
Match: RHA1A_ARATH (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana GN=RHA1A PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.500e-16
Identity = 43/127 (33.86%), Postives = 77/127 (60.63%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILE-SSINWPEPEPESKPASASIIQEMLPV 61
           +P +++  L ++ F R  +  L   +GL   L+ +  + P+    +   SAS+  E++PV
Sbjct: 13  IPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALSTSASLANELIPV 72

Query: 62  MKFTDLECGTTPEQ-CAVCLNEFKSHEEIRVLTNCNHVFHRACVDSW-MNHDQISCPLCR 121
           ++F+DL   T PE  C VCL++F+S +++R L  C HVFH  C+D W ++++++ CP+CR
Sbjct: 73  VRFSDLP--TDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWIVDYNKMKCPVCR 132

Query: 122 THIVPNE 126
              +P E
Sbjct: 133 HRFLPKE 137

[Close]

BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
Match: RHA1B_ARATH (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana GN=RHA1B PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.700e-16
Identity = 45/127 (35.43%), Postives = 77/127 (60.63%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILE-SSINWPEPEPESKPASASIIQEMLPV 61
           +P +++ TL ++ F R  +  L   +GL   L+ +  +  +P   +   SA++  E++PV
Sbjct: 12  IPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALSTSATLANELIPV 71

Query: 62  MKFTDLECGTTPEQ-CAVCLNEFKSHEEIRVLTNCNHVFHRACVDSW-MNHDQISCPLCR 121
           ++F+DL   T PE  C VCL++F S ++IR L  C HVFH  C+D W ++ ++I+CP+CR
Sbjct: 72  VRFSDLL--TDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDCNKITCPICR 131

Query: 122 THIVPNE 126
              +P E
Sbjct: 132 NRFLPEE 136

[Close]

BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
Match: ATL59_ARATH (E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana GN=ATL59 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.000e-12
Identity = 31/98 (31.63%), Postives = 59/98 (60.20%), Query Frame = 1


		  
Query: 54  IQEMLPVMKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQIS 113
           I+EMLP++ + +        QC+VCL ++++ E+++ + +C H FH  C+D W+     +
Sbjct: 77  IREMLPIVIYKE-SFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLWLT-SHTT 136

Query: 114 CPLCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFS 152
           CPLCR  ++P   +++ H+ +E +++I      GGE S
Sbjct: 137 CPLCRLSLIPKPSVDLSHQSIEIVSSIENT--NGGEAS 170

[Close]

BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
Match: ATL7_ARATH (RING-H2 finger protein ATL7 OS=Arabidopsis thaliana GN=ATL7 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.800e-12
Identity = 33/98 (33.67%), Postives = 58/98 (59.18%), Query Frame = 1


		  
Query: 54  IQEMLPVMKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQIS 113
           I+EMLPV+ + +        QC+VCL ++++ E+++ + +C H FH  C+D W+     +
Sbjct: 91  IREMLPVVIYKESFI-VKDSQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLWLT-SHTT 150

Query: 114 CPLCRTHIVPNELLEVFHEKVEALAAISGVLEFGGEFS 152
           CPLCR  ++P   L++ H+  E +++I      GGE S
Sbjct: 151 CPLCRLSLIPKPSLDLSHQSTEIVSSIEN--SNGGEAS 184

[Close]

BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
Match: ATL15_ARATH (E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana GN=ATL15 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.300e-12
Identity = 32/89 (35.96%), Postives = 49/89 (55.06%), Query Frame = 1


		  
Query: 38  NWPEPEPESKPASASIIQEMLPVMKFTD---LECGTTPEQCAVCLNEFKSHEEIRVLTNC 97
           NW      ++P   + + E  P   ++    L  G    +C VCLNEF+  E +R++  C
Sbjct: 78  NWLNVRQTTEPGLDASVIETFPTFPYSTVKTLRIGKEALECPVCLNEFEDDETLRLIPQC 137

Query: 98  NHVFHRACVDSWMNHDQISCPLCRTHIVP 124
            HVFH  C+D+W+   Q +CPLCR ++VP
Sbjct: 138 CHVFHPGCIDAWL-RSQTTCPLCRANLVP 165

[Close]

BLAST of Spo28091.1 vs. TAIR (Arabidopsis)
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2)

HSP 1 Score: 137.5 bits (345), Expect = 7.300e-33
Identity = 74/155 (47.74%), Postives = 98/155 (63.23%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILESSINWPE--------PEPESKPASASI 61
           LP   + TLS+L F R  + ++ R LGLS  LE    WP+        PE  S P SA +
Sbjct: 12  LPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIPETRS-PFSALL 71

Query: 62  IQEMLPVMKFTDLECG--TTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSWMNHDQ 121
           I+E+LPV+KF +L       PE CAVCL EF+  +EIR L NC H+FHR+C+D WM+HDQ
Sbjct: 72  IREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDHDQ 131

Query: 122 ISCPLCRTHIVPNELLEVFHEKVEALAAISGVLEF 147
            +CPLCRT  VP+E+ E F+++   L A SGV +F
Sbjct: 132 KTCPLCRTPFVPDEMQEEFNQR---LWAASGVHDF 162

[Close]

BLAST of Spo28091.1 vs. TAIR (Arabidopsis)
Match: AT1G63840.1 (RING/U-box superfamily protein)

HSP 1 Score: 115.5 bits (288), Expect = 3.000e-26
Identity = 61/140 (43.57%), Postives = 92/140 (65.71%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILES---SINWPEPEPE-SKPASASIIQ- 60
           +LP      LS L   R  I T+ +++GL   LE    S +WP+P P  +KP SA+I+  
Sbjct: 11  LLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPEPVSTSWPDPPPTLTKPDSAAILAG 70

Query: 61  EMLPVMKFTDLECGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHRACVDSW-MNHDQISC 120
           EMLPV++F+D+    + E CAVCL +F++ +EIR LTNC H+FHR C+D W M ++Q++C
Sbjct: 71  EMLPVVRFSDINRPES-ECCAVCLYDFENDDEIRRLTNCRHIFHRGCLDRWMMGYNQMTC 130

Query: 121 PLCRTHIVPNELLEVFHEKV 135
           PLCRT  +P+ L   F++++
Sbjct: 131 PLCRTQFIPDHLQLEFNQRL 149

[Close]

BLAST of Spo28091.1 vs. TAIR (Arabidopsis)
Match: AT5G41400.1 (RING/U-box superfamily protein)

HSP 1 Score: 108.6 bits (270), Expect = 3.600e-24
Identity = 63/155 (40.65%), Postives = 93/155 (60.00%), Query Frame = 1


		  
Query: 1   MLPYFLINTLSLLSFSRPFILTLLRLLGLSHILES-----SINWPEPEPESK-------- 60
           +LP   ++ LSLL   R  I T  R+LGL   LES     S +W EP   S         
Sbjct: 11  LLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYMSTAAHHHQES 70

Query: 61  ----PASASIIQEMLPVMKFTDLE---CGTTPEQCAVCLNEFKSHEEIRVLTNCNHVFHR 120
               P +A +  E+LPV++F++L     G+  + CAVCL+EF++ +EIR LTNC H+FHR
Sbjct: 71  SFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRRLTNCQHIFHR 130

Query: 121 ACVDSW-MNHDQISCPLCRTHIVPNELLEVFHEKV 135
           +C+D W M ++Q++CPLCRT  + +EL   F+++V
Sbjct: 131 SCLDRWMMGYNQMTCPLCRTPFISDELQVAFNQRV 165

[Close]

BLAST of Spo28091.1 vs. TAIR (Arabidopsis)
Match: AT4G11370.1 (RING-H2 finger A1A)

HSP 1 Score: 85.9 bits (211), Expect = 2.500e-17
Identity = 43/127 (33.86%), Postives = 77/127 (60.63%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILE-SSINWPEPEPESKPASASIIQEMLPV 61
           +P +++  L ++ F R  +  L   +GL   L+ +  + P+    +   SAS+  E++PV
Sbjct: 13  IPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALSTSASLANELIPV 72

Query: 62  MKFTDLECGTTPEQ-CAVCLNEFKSHEEIRVLTNCNHVFHRACVDSW-MNHDQISCPLCR 121
           ++F+DL   T PE  C VCL++F+S +++R L  C HVFH  C+D W ++++++ CP+CR
Sbjct: 73  VRFSDLP--TDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWIVDYNKMKCPVCR 132

Query: 122 THIVPNE 126
              +P E
Sbjct: 133 HRFLPKE 137

[Close]

BLAST of Spo28091.1 vs. TAIR (Arabidopsis)
Match: AT4G11360.1 (RING-H2 finger A1B)

HSP 1 Score: 85.1 bits (209), Expect = 4.300e-17
Identity = 45/127 (35.43%), Postives = 77/127 (60.63%), Query Frame = 1


		  
Query: 2   LPYFLINTLSLLSFSRPFILTLLRLLGLSHILE-SSINWPEPEPESKPASASIIQEMLPV 61
           +P +++ TL ++ F R  +  L   +GL   L+ +  +  +P   +   SA++  E++PV
Sbjct: 12  IPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALSTSATLANELIPV 71

Query: 62  MKFTDLECGTTPEQ-CAVCLNEFKSHEEIRVLTNCNHVFHRACVDSW-MNHDQISCPLCR 121
           ++F+DL   T PE  C VCL++F S ++IR L  C HVFH  C+D W ++ ++I+CP+CR
Sbjct: 72  VRFSDLL--TDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDCNKITCPICR 131

Query: 122 THIVPNE 126
              +P E
Sbjct: 132 NRFLPEE 136

The following BLAST results are available for this feature:

BLAST of Spo28091.1 vs. NCBI nr
BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
BLAST of Spo28091.1 vs. TAIR (Arabidopsis)

BLAST of Spo28091.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902239639\|gb\|KNA25665.1\|	3.4e-88	99.3	hypothetical protein SOVF_0044...	[more]
gi\|731331031\|ref\|XP_010676446.1\|	2.2e-55	69.6	PREDICTED: E3 ubiquitin-protei...	[more]
gi\|703162027\|ref\|XP_010112947.1\|	1.5e-35	50.3	E3 ubiquitin-protein ligase RH...	[more]
gi\|567904996\|ref\|XP_006444986.1\|	2.5e-35	51.9	hypothetical protein CICLE_v10...	[more]
gi\|255537543\|ref\|XP_002509838.1\|	3.3e-35	50.6	PREDICTED: E3 ubiquitin-protei...	[more]

BLAST of Spo28091.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9S1P2_SPIOL	2.4e-88	99.3	Uncharacterized protein OS=Spi...	[more]
W9SMG3_9ROSA	1.0e-35	50.3	E3 ubiquitin-protein ligase RH...	[more]
A0A067H5S5_CITSI	1.8e-35	51.9	Uncharacterized protein OS=Cit...	[more]
V4U7S5_9ROSI	1.8e-35	51.9	Uncharacterized protein OS=Cit...	[more]
B9RCF4_RICCO	2.3e-35	50.6	Zinc finger protein, putative ...	[more]

BLAST of Spo28091.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RHA1A_ARATH	4.5e-16	33.8	Probable E3 ubiquitin-protein ...	[more]
RHA1B_ARATH	7.7e-16	35.4	E3 ubiquitin-protein ligase RH...	[more]
ATL59_ARATH	1.0e-12	31.6	E3 ubiquitin-protein ligase AT...	[more]
ATL7_ARATH	1.8e-12	33.6	RING-H2 finger protein ATL7 OS...	[more]
ATL15_ARATH	2.3e-12	35.9	E3 ubiquitin-protein ligase AT...	[more]

BLAST of Spo28091.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G61460.1	7.3e-33	47.7	brassinosteroid-responsive RIN...	[more]
AT1G63840.1	3.0e-26	43.5	RING/U-box superfamily protein	[more]
AT5G41400.1	3.6e-24	40.6	RING/U-box superfamily protein	[more]
AT4G11370.1	2.5e-17	33.8	RING-H2 finger A1A	[more]
AT4G11360.1	4.3e-17	35.4	RING-H2 finger A1B	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 74..118 score: 5.8
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 75..117 score: 3.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 75..118 score: 13
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10		coord: 72..121 score: 2.8
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 40..143 score: 1.1
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 74..124 score: 6.83

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding