Spo28189.1 (mRNA) | SpinachBase

Overview


Name	Spo28189.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Sigma factor binding protein 1, chloroplastic (AtsibI) (Sigma factor binding protein I) (SigA binding protein) (Precursor)
Location	SpoScf_00309 : 417333 .. 417917 (+)
Sequence length	585

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGATGTTAGCCAAAACCCTAGTTTTCACCTCTCAAAACCTCCCCATAAGCTAAAAACTAAACCCACCAAAAGAACAACAAGATCTAGAAACAAGAATAACAACACGGCTACCACGGCCGCCTCCACCACCAACAACAACCACAACAAGCCACTCAAGGTGGTTCACATCTCTAACCCTATGAAGTTCCAAGTCGCCGCAGCTGGGTTTCGAGCCCTTGTCCAAGGGCTCACCGGCCGCGACGCCGTAATCGACTGGCCTGAGGAACACCAACCCGACTTCGAAGTTGTGGCGGCTGCTGCTGGCGTTGCTGAAGATGATACTGCTGAGGATGATAGTAGTGCTAACAGTACGGATGATCAGGTAAATGATCACCACCATCATCAGCATCGTGATGAGGTTGTTAATATGTCTGCCTGTTTCGGGCCGTCCGATCATTATAATCTGATGAGCTTTGATTATTACGGGGCCCACTTTAATGGTGACGTCATTGTGGATGACGATGTTGAACAAAGCATGGTTGTTATGGATGATTTCCGCGCTTTTTTGGGGGATGTTAATCAATCATTACTTCATTATTAA

mRNA sequence

ATGTCAGATGTTAGCCAAAACCCTAGTTTTCACCTCTCAAAACCTCCCCATAAGCTAAAAACTAAACCCACCAAAAGAACAACAAGATCTAGAAACAAGAATAACAACACGGCTACCACGGCCGCCTCCACCACCAACAACAACCACAACAAGCCACTCAAGGTGGTTCACATCTCTAACCCTATGAAGTTCCAAGTCGCCGCAGCTGGGTTTCGAGCCCTTGTCCAAGGGCTCACCGGCCGCGACGCCGTAATCGACTGGCCTGAGGAACACCAACCCGACTTCGAAGTTGTGGCGGCTGCTGCTGGCGTTGCTGAAGATGATACTGCTGAGGATGATAGTAGTGCTAACAGTACGGATGATCAGGTAAATGATCACCACCATCATCAGCATCGTGATGAGGTTGTTAATATGTCTGCCTGTTTCGGGCCGTCCGATCATTATAATCTGATGAGCTTTGATTATTACGGGGCCCACTTTAATGGTGACGTCATTGTGGATGACGATGTTGAACAAAGCATGGTTGTTATGGATGATTTCCGCGCTTTTTTGGGGGATGTTAATCAATCATTACTTCATTATTAA

Coding sequence (CDS)

ATGTCAGATGTTAGCCAAAACCCTAGTTTTCACCTCTCAAAACCTCCCCATAAGCTAAAAACTAAACCCACCAAAAGAACAACAAGATCTAGAAACAAGAATAACAACACGGCTACCACGGCCGCCTCCACCACCAACAACAACCACAACAAGCCACTCAAGGTGGTTCACATCTCTAACCCTATGAAGTTCCAAGTCGCCGCAGCTGGGTTTCGAGCCCTTGTCCAAGGGCTCACCGGCCGCGACGCCGTAATCGACTGGCCTGAGGAACACCAACCCGACTTCGAAGTTGTGGCGGCTGCTGCTGGCGTTGCTGAAGATGATACTGCTGAGGATGATAGTAGTGCTAACAGTACGGATGATCAGGTAAATGATCACCACCATCATCAGCATCGTGATGAGGTTGTTAATATGTCTGCCTGTTTCGGGCCGTCCGATCATTATAATCTGATGAGCTTTGATTATTACGGGGCCCACTTTAATGGTGACGTCATTGTGGATGACGATGTTGAACAAAGCATGGTTGTTATGGATGATTTCCGCGCTTTTTTGGGGGATGTTAATCAATCATTACTTCATTATTAA

Protein sequence

MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISNPMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTDDQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDFRAFLGDVNQSLLHY

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo28189	Spo28189	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo28189.1	Spo28189.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo28189.1.exon.1	Spo28189.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo28189.1.CDS.1	Spo28189.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo28189.1 vs. NCBI nr
Match: gi|902170544|gb|KNA07853.1| (hypothetical protein SOVF_167920 [Spinacia oleracea])

HSP 1 Score: 383.6 bits (984), Expect = 2.000e-103
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 1


		  
Query: 1   MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISN 60
           MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISN
Sbjct: 1   MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISN 60

Query: 61  PMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTD 120
           PMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTD
Sbjct: 61  PMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTD 120

Query: 121 DQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDF 180
           DQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDF
Sbjct: 121 DQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDF 180

Query: 181 RAFLGDVNQSLLHY 195
           RAFLGDVNQSLLHY
Sbjct: 181 RAFLGDVNQSLLHY 194

[Close]

BLAST of Spo28189.1 vs. NCBI nr
Match: gi|731324534|ref|XP_010673020.1| (PREDICTED: uncharacterized protein LOC104889490 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 136.3 bits (342), Expect = 5.500e-29
Identity = 93/199 (46.73%), Postives = 117/199 (58.79%), Query Frame = 1


		  
Query: 1   MSDVSQNPSFHLSKPPHKL-------KTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPL 60
           MS+V+QNP+FHLSKPPHKL       K KPT + +     NNNT+T+  + TN N NKPL
Sbjct: 1   MSEVTQNPNFHLSKPPHKLTNKQTMFKNKPTTKRSGKPKYNNNTSTSTNTNTNTN-NKPL 60

Query: 61  KVVHISNPMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDD 120
           KVVHISNPMKFQ  AA FR LVQGLTGRDAV  WP+E   + + V A           +D
Sbjct: 61  KVVHISNPMKFQATAAEFRDLVQGLTGRDAVFKWPDEQLYEVQQVNAEI---------ED 120

Query: 121 SSANSTDDQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQS 180
                 DD +    H Q   + V++SACFGP+DH  L  +DY+         VD   E  
Sbjct: 121 CGVTINDDLI---LHEQVVHKEVDLSACFGPNDH--LGGYDYF---------VD---EHH 172

Query: 181 MVVMDDFRAFLGDVNQSLL 193
           +VVM+D RAF+G  +  L+
Sbjct: 181 LVVMEDLRAFIGADDDDLM 172

[Close]

BLAST of Spo28189.1 vs. NCBI nr
Match: gi|870864049|gb|KMT15182.1| (hypothetical protein BVRB_3g062950 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 74.7 bits (182), Expect = 2.000e-10
Identity = 53/131 (40.46%), Postives = 69/131 (52.67%), Query Frame = 1


		  
Query: 62  MKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTDD 121
           MKFQ  AA FR LVQGLTGRDAV  WP+E   + + V A           +D      DD
Sbjct: 1   MKFQATAAEFRDLVQGLTGRDAVFKWPDEQLYEVQQVNAEI---------EDCGVTINDD 60

Query: 122 QVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDFR 181
            +    H Q   + V++SACFGP+DH  L  +DY+         VD   E  +VVM+D R
Sbjct: 61  LI---LHEQVVHKEVDLSACFGPNDH--LGGYDYF---------VD---EHHLVVMEDLR 105

Query: 182 AFLGDVNQSLL 193
           AF+G  +  L+
Sbjct: 121 AFIGADDDDLM 105

[Close]

BLAST of Spo28189.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QME1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_167920 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 1.400e-103
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 1


		  
Query: 1   MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISN 60
           MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISN
Sbjct: 1   MSDVSQNPSFHLSKPPHKLKTKPTKRTTRSRNKNNNTATTAASTTNNNHNKPLKVVHISN 60

Query: 61  PMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTD 120
           PMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTD
Sbjct: 61  PMKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTD 120

Query: 121 DQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDF 180
           DQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDF
Sbjct: 121 DQVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDF 180

Query: 181 RAFLGDVNQSLLHY 195
           RAFLGDVNQSLLHY
Sbjct: 181 RAFLGDVNQSLLHY 194

[Close]

BLAST of Spo28189.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CNT4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g062950 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.400e-10
Identity = 53/131 (40.46%), Postives = 69/131 (52.67%), Query Frame = 1


		  
Query: 62  MKFQVAAAGFRALVQGLTGRDAVIDWPEEHQPDFEVVAAAAGVAEDDTAEDDSSANSTDD 121
           MKFQ  AA FR LVQGLTGRDAV  WP+E   + + V A           +D      DD
Sbjct: 1   MKFQATAAEFRDLVQGLTGRDAVFKWPDEQLYEVQQVNAEI---------EDCGVTINDD 60

Query: 122 QVNDHHHHQHRDEVVNMSACFGPSDHYNLMSFDYYGAHFNGDVIVDDDVEQSMVVMDDFR 181
            +    H Q   + V++SACFGP+DH  L  +DY+         VD   E  +VVM+D R
Sbjct: 61  LI---LHEQVVHKEVDLSACFGPNDH--LGGYDYF---------VD---EHHLVVMEDLR 105

Query: 182 AFLGDVNQSLL 193
           AF+G  +  L+
Sbjct: 121 AFIGADDDDLM 105

The following BLAST results are available for this feature:

BLAST of Spo28189.1 vs. NCBI nr
BLAST of Spo28189.1 vs. UniProtKB/TrEMBL
BLAST of Spo28189.1 vs. ExPASy Swiss-Prot
BLAST of Spo28189.1 vs. TAIR (Arabidopsis)

BLAST of Spo28189.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 3


Match Name	E-value	Identity	Description
gi\|902170544\|gb\|KNA07853.1\|	2.0e-103	100.	hypothetical protein SOVF_1679...	[more]
gi\|731324534\|ref\|XP_010673020.1\|	5.5e-29	46.7	PREDICTED: uncharacterized pro...	[more]
gi\|870864049\|gb\|KMT15182.1\|	2.0e-10	40.4	hypothetical protein BVRB_3g06...	[more]

BLAST of Spo28189.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 2


Match Name	E-value	Identity	Description
A0A0K9QME1_SPIOL	1.4e-103	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CNT4_BETVU	1.4e-10	40.4	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo28189.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo28189.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008889	VQ	PFAM	PF05678	VQ	coord: 58..83 score: 3.
None	No IPR available	PANTHER	PTHR33624	FAMILY NOT NAMED	coord: 27..155 score: 2.4
None	No IPR available	PANTHER	PTHR33624:SF2	SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTIC-RELATED	coord: 27..155 score: 2.4