Spo27270 (gene) | SpinachBase

Overview


Name	Spo27270
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	B12D-like protein
Location	chr5 : 8357190 .. 8357777 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTCTGTGAATCGGTGGTTGAAACCAGAGGTTTATCCTCTGTTTGCTGCAGTTGGAGTTGCTGTTGGTATCTGTGGTTTTCAGCTCGTCCGTAACATCTGCATCAACCCTGAAGTCAGGTACTTTTTATATTATTCATTCCTACGTTTTTATTTTCTTGTTAATTGATCGTAGTGTAACAGTTTTGCTGATGTTGTTTTGGTTAAACCAGAGTTAGCAAGGAGAACAGAGCAGCTGGAGTTCTGGATAATTTTTCAGAGGGGGAGAAATACACTGAGCATTTCCTCAGGAAATATGTTCGTAACAAGACACCGGAGATCATGCCTTCTATCAACAGCTTTTTCGCAGATCCAAAATCATCGTAGCTGATCTGATTCTTGAAAGCTAGCTAAGCTGCCTTCAGAGTTCAGTCCCGTTTTTTTCTGTTGTAATATGTGATCATTAGACTACAGCGTTACCCCATGATTTGATAAACAAACTTTTCTCCATTGTATCCAATCAAGTTGGAATTTAAACTCACTTGCTGTTTGTTTTGGTTTCTTTTTTCTCTATACTGGACAATAATTGAAAAAATCCACATTGTAG

mRNA sequence

ATGGGCTCTGTGAATCGGTGGTTGAAACCAGAGGTTTATCCTCTGTTTGCTGCAGTTGGAGTTGCTGTTGGTATCTGTGGTTTTCAGCTCGTCCGTAACATCTGCATCAACCCTGAAGTCAGAGTTAGCAAGGAGAACAGAGCAGCTGGAGTTCTGGATAATTTTTCAGAGGGGGAGAAATACACTGAGCATTTCCTCAGGAAATATGTTCGTAACAAGACACCGGAGATCATGCCTTCTATCAACAGCTTTTTCGCAGATCCAAAATCATCGTAGCTGATCTGATTCTTGAAAGCTAGCTAAGCTGCCTTCAGAGTTCAGTCCCGTTTTTTTCTGTTGTAATATGTGATCATTAGACTACAGCGTTACCCCATGATTTGATAAACAAACTTTTCTCCATTGTATCCAATCAAGTTGGAATTTAAACTCACTTGCTGTTTGTTTTGGTTTCTTTTTTCTCTATACTGGACAATAATTGAAAAAATCCACATTGTAG

Coding sequence (CDS)

ATGGGCTCTGTGAATCGGTGGTTGAAACCAGAGGTTTATCCTCTGTTTGCTGCAGTTGGAGTTGCTGTTGGTATCTGTGGTTTTCAGCTCGTCCGTAACATCTGCATCAACCCTGAAGTCAGAGTTAGCAAGGAGAACAGAGCAGCTGGAGTTCTGGATAATTTTTCAGAGGGGGAGAAATACACTGAGCATTTCCTCAGGAAATATGTTCGTAACAAGACACCGGAGATCATGCCTTCTATCAACAGCTTTTTCGCAGATCCAAAATCATCGTAG

Protein sequence

MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEHFLRKYVRNKTPEIMPSINSFFADPKSS

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo27270.1	Spo27270.1	mRNA

Homology

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BLAST of Spo27270.1 vs. NCBI nr
Match: gi|902205077|gb|KNA15084.1| (hypothetical protein SOVF_101540 [Spinacia oleracea])

HSP 1 Score: 190.7 bits (483), Expect = 1.200e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1

		  
Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK
Sbjct: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 92
          YTEHFLRKYVRNKTPEIMPSINSFFADPKSS
Sbjct: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 91

[Close]

BLAST of Spo27270.1 vs. NCBI nr
Match: gi|731335322|ref|XP_010678668.1| (PREDICTED: uncharacterized protein LOC104894188 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 175.3 bits (443), Expect = 5.000e-41
Identity = 82/88 (93.18%), Postives = 85/88 (96.59%), Query Frame = 1

		  
Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADP 89
          Y EHFLRKYVRNKTPEIMPSINSFFADP
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFADP 88

[Close]

BLAST of Spo27270.1 vs. NCBI nr
Match: gi|823121374|ref|XP_012466012.1| (PREDICTED: uncharacterized protein LOC105784633 [Gossypium raimondii])

HSP 1 Score: 174.1 bits (440), Expect = 1.100e-40
Identity = 80/85 (94.12%), Postives = 84/85 (98.82%), Query Frame = 1

		  
Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          NRWL+PEVYPLFAAVGVAVGICGFQLVRNICINPEVRV+KENRAAGVLDNF+EGEKY+EH
Sbjct: 3  NRWLRPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVTKENRAAGVLDNFAEGEKYSEH 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADPK 90
          FLRKYVRNKTPEIMPSINSFF DPK
Sbjct: 63 FLRKYVRNKTPEIMPSINSFFTDPK 87

[Close]

BLAST of Spo27270.1 vs. NCBI nr
Match: gi|99646724|emb|CAK22419.1| (B12D-like protein [Beta vulgaris])

HSP 1 Score: 172.6 bits (436), Expect = 3.300e-40
Identity = 81/87 (93.10%), Postives = 84/87 (96.55%), Query Frame = 1

		  
Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFAD 88
          Y EHFLRKYVRNKTPEIMPSINSFFAD
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFAD 87

[Close]

BLAST of Spo27270.1 vs. NCBI nr
Match: gi|225457158|ref|XP_002283743.1| (PREDICTED: uncharacterized protein LOC100263922 [Vitis vinifera])

HSP 1 Score: 168.3 bits (425), Expect = 6.100e-39
Identity = 75/89 (84.27%), Postives = 88/89 (98.88%), Query Frame = 1

		  
Query: 3  SVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYT 62
          ++NRWL+PEVYPLFAA+GVAVGICGFQLVRNICINP+VRVSKENRAAGVL+NF+EG+KY+
Sbjct: 5  TMNRWLRPEVYPLFAAMGVAVGICGFQLVRNICINPDVRVSKENRAAGVLENFTEGQKYS 64

Query: 63 EHFLRKYVRNKTPEIMPSINSFFADPKSS 92
          EHFLRKYVRNK+PEIMPSINSFF+DP+ +
Sbjct: 65 EHFLRKYVRNKSPEIMPSINSFFSDPEKN 93

[Close]

BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R7X1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_101540 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 8.100e-46
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1

		  
Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK
Sbjct: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 92
          YTEHFLRKYVRNKTPEIMPSINSFFADPKSS
Sbjct: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 91

[Close]

BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CE95_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g116080 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 3.500e-41
Identity = 82/88 (93.18%), Postives = 85/88 (96.59%), Query Frame = 1

		  
Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADP 89
          Y EHFLRKYVRNKTPEIMPSINSFFADP
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFADP 88

[Close]

BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0B0PNM1_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_04740 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 7.800e-41
Identity = 80/85 (94.12%), Postives = 84/85 (98.82%), Query Frame = 1

		  
Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          NRWL+PEVYPLFAAVGVAVGICGFQLVRNICINPEVRV+KENRAAGVLDNF+EGEKY+EH
Sbjct: 3  NRWLRPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVTKENRAAGVLDNFAEGEKYSEH 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADPK 90
          FLRKYVRNKTPEIMPSINSFF DPK
Sbjct: 63 FLRKYVRNKTPEIMPSINSFFTDPK 87

[Close]

BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0D2N5K0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G047700 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 7.800e-41
Identity = 80/85 (94.12%), Postives = 84/85 (98.82%), Query Frame = 1

		  
Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          NRWL+PEVYPLFAAVGVAVGICGFQLVRNICINPEVRV+KENRAAGVLDNF+EGEKY+EH
Sbjct: 3  NRWLRPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVTKENRAAGVLDNFAEGEKYSEH 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADPK 90
          FLRKYVRNKTPEIMPSINSFF DPK
Sbjct: 63 FLRKYVRNKTPEIMPSINSFFTDPK 87

[Close]

BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: Q1H8M8_BETVU (B12D-like protein OS=Beta vulgaris GN=b12D PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.300e-40
Identity = 81/87 (93.10%), Postives = 84/87 (96.55%), Query Frame = 1

		  
Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFAD 88
          Y EHFLRKYVRNKTPEIMPSINSFFAD
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFAD 87

[Close]

BLAST of Spo27270.1 vs. TAIR (Arabidopsis)
Match: AT3G48140.1 (B12D protein)

HSP 1 Score: 141.0 bits (354), Expect = 3.700e-34
Identity = 63/84 (75.00%), Postives = 72/84 (85.71%), Query Frame = 1

		  
Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          +RWL+PEVYPLFAA GVAVGIC F L+RNI  NPEVR +KENRAAG+LDN +EGEKY E+
Sbjct: 3  SRWLRPEVYPLFAATGVAVGICAFSLIRNITGNPEVRCTKENRAAGILDNHAEGEKYKEN 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADP 89
          FLRK+VRNK PEIMP +N FF DP
Sbjct: 63 FLRKFVRNKKPEIMPGLNKFFTDP 86

[Close]

BLAST of Spo27270.1 vs. TAIR (Arabidopsis)
Match: AT3G29970.1 (B12D protein)

HSP 1 Score: 102.1 bits (253), Expect = 1.900e-22
Identity = 41/83 (49.40%), Postives = 60/83 (72.29%), Query Frame = 1

		  
Query: 4  VNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTE 63
          + RW++PEVYPL  A+G    +  FQL RN  +NP+ R++KE+R  G+L+N  EGEKY +
Sbjct: 1  MGRWVRPEVYPLLGAMGFVTSMVVFQLTRNAFLNPDCRINKEHRKMGILENKDEGEKYAQ 60

Query: 64 HFLRKYVRNKTPEIMPSINSFFA 87
          H  RKY+R + P++MPS+N FF+
Sbjct: 61 HNFRKYLRTRQPQVMPSLNRFFS 83

The following BLAST results are available for this feature:

BLAST of Spo27270.1 vs. NCBI nr
BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
BLAST of Spo27270.1 vs. ExPASy Swiss-Prot
BLAST of Spo27270.1 vs. TAIR (Arabidopsis)

BLAST of Spo27270.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|902205077\|gb\|KNA15084.1\|	1.2e-45	100.	hypothetical protein SOVF_1015...	[more]
gi\|731335322\|ref\|XP_010678668.1\|	5.0e-41	93.1	PREDICTED: uncharacterized pro...	[more]
gi\|823121374\|ref\|XP_012466012.1\|	1.1e-40	94.1	PREDICTED: uncharacterized pro...	[more]
gi\|99646724\|emb\|CAK22419.1\|	3.3e-40	93.1	B12D-like protein [Beta vulgar...	[more]
gi\|225457158\|ref\|XP_002283743.1\|	6.1e-39	84.2	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
A0A0K9R7X1_SPIOL	8.1e-46	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CE95_BETVU	3.5e-41	93.1	Uncharacterized protein OS=Bet...	[more]
A0A0B0PNM1_GOSAR	7.8e-41	94.1	Uncharacterized protein OS=Gos...	[more]
A0A0D2N5K0_GOSRA	7.8e-41	94.1	Uncharacterized protein OS=Gos...	[more]
Q1H8M8_BETVU	2.3e-40	93.1	B12D-like protein OS=Beta vulg...	[more]

BLAST of Spo27270.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description

BLAST of Spo27270.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
AT3G48140.1	3.7e-34	75.0	B12D protein	[more]
AT3G29970.1	1.9e-22	49.4	B12D protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010530	NADH-ubiquinone reductase complex 1 MLRQ subunit	PFAM	PF06522	B12D	coord: 9..76 score: 8.4
None	No IPR available	PANTHER	PTHR33417	FAMILY NOT NAMED	coord: 4..89 score: 2.2
None	No IPR available	PANTHER	PTHR33417:SF2	SUBFAMILY NOT NAMED	coord: 4..89 score: 2.2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0006098	pentose-phosphate shunt
biological_process	GO:0006783	heme biosynthetic process
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009156	ribonucleoside monophosphate biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0009165	nucleotide biosynthetic process
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0032259	methylation
biological_process	GO:0006694	steroid biosynthetic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0044424	intracellular part
molecular_function	GO:0004749	ribose phosphate diphosphokinase activity
molecular_function	GO:0016757	transferase activity, transferring glycosyl groups
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0016901	oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor
molecular_function	GO:0048038	quinone binding
molecular_function	GO:0016773	phosphotransferase activity, alcohol group as acceptor
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0019904	protein domain specific binding
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0038023	signaling receptor activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008168	methyltransferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo04884	0.76	Barchart \| Table
Spo09393	0.73	Barchart \| Table
Spo20259	0.71	Barchart \| Table
Spo04870	0.71	Barchart \| Table
Spo03507	0.70	Barchart \| Table
Spo00371	0.67	Barchart \| Table
Spo23325	0.67	Barchart \| Table
Spo11493	0.66	Barchart \| Table
Spo23832	0.66	Barchart \| Table
Spo18333	0.65	Barchart \| Table
Spo02581	0.65	Barchart \| Table
Spo08326	0.65	Barchart \| Table