Spo27270 (gene)

Overview
NameSpo27270
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionB12D-like protein
Locationchr5 : 8357190 .. 8357777 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTCTGTGAATCGGTGGTTGAAACCAGAGGTTTATCCTCTGTTTGCTGCAGTTGGAGTTGCTGTTGGTATCTGTGGTTTTCAGCTCGTCCGTAACATCTGCATCAACCCTGAAGTCAGGTACTTTTTATATTATTCATTCCTACGTTTTTATTTTCTTGTTAATTGATCGTAGTGTAACAGTTTTGCTGATGTTGTTTTGGTTAAACCAGAGTTAGCAAGGAGAACAGAGCAGCTGGAGTTCTGGATAATTTTTCAGAGGGGGAGAAATACACTGAGCATTTCCTCAGGAAATATGTTCGTAACAAGACACCGGAGATCATGCCTTCTATCAACAGCTTTTTCGCAGATCCAAAATCATCGTAGCTGATCTGATTCTTGAAAGCTAGCTAAGCTGCCTTCAGAGTTCAGTCCCGTTTTTTTCTGTTGTAATATGTGATCATTAGACTACAGCGTTACCCCATGATTTGATAAACAAACTTTTCTCCATTGTATCCAATCAAGTTGGAATTTAAACTCACTTGCTGTTTGTTTTGGTTTCTTTTTTCTCTATACTGGACAATAATTGAAAAAATCCACATTGTAG

mRNA sequence

ATGGGCTCTGTGAATCGGTGGTTGAAACCAGAGGTTTATCCTCTGTTTGCTGCAGTTGGAGTTGCTGTTGGTATCTGTGGTTTTCAGCTCGTCCGTAACATCTGCATCAACCCTGAAGTCAGAGTTAGCAAGGAGAACAGAGCAGCTGGAGTTCTGGATAATTTTTCAGAGGGGGAGAAATACACTGAGCATTTCCTCAGGAAATATGTTCGTAACAAGACACCGGAGATCATGCCTTCTATCAACAGCTTTTTCGCAGATCCAAAATCATCGTAGCTGATCTGATTCTTGAAAGCTAGCTAAGCTGCCTTCAGAGTTCAGTCCCGTTTTTTTCTGTTGTAATATGTGATCATTAGACTACAGCGTTACCCCATGATTTGATAAACAAACTTTTCTCCATTGTATCCAATCAAGTTGGAATTTAAACTCACTTGCTGTTTGTTTTGGTTTCTTTTTTCTCTATACTGGACAATAATTGAAAAAATCCACATTGTAG

Coding sequence (CDS)

ATGGGCTCTGTGAATCGGTGGTTGAAACCAGAGGTTTATCCTCTGTTTGCTGCAGTTGGAGTTGCTGTTGGTATCTGTGGTTTTCAGCTCGTCCGTAACATCTGCATCAACCCTGAAGTCAGAGTTAGCAAGGAGAACAGAGCAGCTGGAGTTCTGGATAATTTTTCAGAGGGGGAGAAATACACTGAGCATTTCCTCAGGAAATATGTTCGTAACAAGACACCGGAGATCATGCCTTCTATCAACAGCTTTTTCGCAGATCCAAAATCATCGTAG

Protein sequence

MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEHFLRKYVRNKTPEIMPSINSFFADPKSS
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo27270.1Spo27270.1mRNA


Homology
[Close]
BLAST of Spo27270.1 vs. NCBI nr
Match: gi|902205077|gb|KNA15084.1| (hypothetical protein SOVF_101540 [Spinacia oleracea])

HSP 1 Score: 190.7 bits (483), Expect = 1.200e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1 

		  

Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK
Sbjct: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 92
          YTEHFLRKYVRNKTPEIMPSINSFFADPKSS
Sbjct: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 91
[Close]
BLAST of Spo27270.1 vs. NCBI nr
Match: gi|731335322|ref|XP_010678668.1| (PREDICTED: uncharacterized protein LOC104894188 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 175.3 bits (443), Expect = 5.000e-41
Identity = 82/88 (93.18%), Postives = 85/88 (96.59%), Query Frame = 1 

		  

Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADP 89
          Y EHFLRKYVRNKTPEIMPSINSFFADP
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFADP 88
[Close]
BLAST of Spo27270.1 vs. NCBI nr
Match: gi|823121374|ref|XP_012466012.1| (PREDICTED: uncharacterized protein LOC105784633 [Gossypium raimondii])

HSP 1 Score: 174.1 bits (440), Expect = 1.100e-40
Identity = 80/85 (94.12%), Postives = 84/85 (98.82%), Query Frame = 1 

		  

Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          NRWL+PEVYPLFAAVGVAVGICGFQLVRNICINPEVRV+KENRAAGVLDNF+EGEKY+EH
Sbjct: 3  NRWLRPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVTKENRAAGVLDNFAEGEKYSEH 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADPK 90
          FLRKYVRNKTPEIMPSINSFF DPK
Sbjct: 63 FLRKYVRNKTPEIMPSINSFFTDPK 87
[Close]
BLAST of Spo27270.1 vs. NCBI nr
Match: gi|99646724|emb|CAK22419.1| (B12D-like protein [Beta vulgaris])

HSP 1 Score: 172.6 bits (436), Expect = 3.300e-40
Identity = 81/87 (93.10%), Postives = 84/87 (96.55%), Query Frame = 1 

		  

Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFAD 88
          Y EHFLRKYVRNKTPEIMPSINSFFAD
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFAD 87
[Close]
BLAST of Spo27270.1 vs. NCBI nr
Match: gi|225457158|ref|XP_002283743.1| (PREDICTED: uncharacterized protein LOC100263922 [Vitis vinifera])

HSP 1 Score: 168.3 bits (425), Expect = 6.100e-39
Identity = 75/89 (84.27%), Postives = 88/89 (98.88%), Query Frame = 1 

		  

Query: 3  SVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYT 62
          ++NRWL+PEVYPLFAA+GVAVGICGFQLVRNICINP+VRVSKENRAAGVL+NF+EG+KY+
Sbjct: 5  TMNRWLRPEVYPLFAAMGVAVGICGFQLVRNICINPDVRVSKENRAAGVLENFTEGQKYS 64

Query: 63 EHFLRKYVRNKTPEIMPSINSFFADPKSS 92
          EHFLRKYVRNK+PEIMPSINSFF+DP+ +
Sbjct: 65 EHFLRKYVRNKSPEIMPSINSFFSDPEKN 93
[Close]
BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R7X1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_101540 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 8.100e-46
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1 

		  

Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK
Sbjct: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 92
          YTEHFLRKYVRNKTPEIMPSINSFFADPKSS
Sbjct: 61 YTEHFLRKYVRNKTPEIMPSINSFFADPKSS 91
[Close]
BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CE95_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g116080 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 3.500e-41
Identity = 82/88 (93.18%), Postives = 85/88 (96.59%), Query Frame = 1 

		  

Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFADP 89
          Y EHFLRKYVRNKTPEIMPSINSFFADP
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFADP 88
[Close]
BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0B0PNM1_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_04740 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 7.800e-41
Identity = 80/85 (94.12%), Postives = 84/85 (98.82%), Query Frame = 1 

		  

Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          NRWL+PEVYPLFAAVGVAVGICGFQLVRNICINPEVRV+KENRAAGVLDNF+EGEKY+EH
Sbjct: 3  NRWLRPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVTKENRAAGVLDNFAEGEKYSEH 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADPK 90
          FLRKYVRNKTPEIMPSINSFF DPK
Sbjct: 63 FLRKYVRNKTPEIMPSINSFFTDPK 87
[Close]
BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: A0A0D2N5K0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G047700 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 7.800e-41
Identity = 80/85 (94.12%), Postives = 84/85 (98.82%), Query Frame = 1 

		  

Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          NRWL+PEVYPLFAAVGVAVGICGFQLVRNICINPEVRV+KENRAAGVLDNF+EGEKY+EH
Sbjct: 3  NRWLRPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVTKENRAAGVLDNFAEGEKYSEH 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADPK 90
          FLRKYVRNKTPEIMPSINSFF DPK
Sbjct: 63 FLRKYVRNKTPEIMPSINSFFTDPK 87
[Close]
BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Match: Q1H8M8_BETVU (B12D-like protein OS=Beta vulgaris GN=b12D PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.300e-40
Identity = 81/87 (93.10%), Postives = 84/87 (96.55%), Query Frame = 1 

		  

Query: 1  MGSVNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEK 60
          M SVNRW++PEVYPLFAAVGVAVGICG QLVRNICINPEVRVSKENRAAGVL+NFSEGEK
Sbjct: 1  MASVNRWVRPEVYPLFAAVGVAVGICGVQLVRNICINPEVRVSKENRAAGVLENFSEGEK 60

Query: 61 YTEHFLRKYVRNKTPEIMPSINSFFAD 88
          Y EHFLRKYVRNKTPEIMPSINSFFAD
Sbjct: 61 YAEHFLRKYVRNKTPEIMPSINSFFAD 87
[Close]
BLAST of Spo27270.1 vs. TAIR (Arabidopsis)
Match: AT3G48140.1 (B12D protein)

HSP 1 Score: 141.0 bits (354), Expect = 3.700e-34
Identity = 63/84 (75.00%), Postives = 72/84 (85.71%), Query Frame = 1 

		  

Query: 5  NRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTEH 64
          +RWL+PEVYPLFAA GVAVGIC F L+RNI  NPEVR +KENRAAG+LDN +EGEKY E+
Sbjct: 3  SRWLRPEVYPLFAATGVAVGICAFSLIRNITGNPEVRCTKENRAAGILDNHAEGEKYKEN 62

Query: 65 FLRKYVRNKTPEIMPSINSFFADP 89
          FLRK+VRNK PEIMP +N FF DP
Sbjct: 63 FLRKFVRNKKPEIMPGLNKFFTDP 86
[Close]
BLAST of Spo27270.1 vs. TAIR (Arabidopsis)
Match: AT3G29970.1 (B12D protein)

HSP 1 Score: 102.1 bits (253), Expect = 1.900e-22
Identity = 41/83 (49.40%), Postives = 60/83 (72.29%), Query Frame = 1 

		  

Query: 4  VNRWLKPEVYPLFAAVGVAVGICGFQLVRNICINPEVRVSKENRAAGVLDNFSEGEKYTE 63
          + RW++PEVYPL  A+G    +  FQL RN  +NP+ R++KE+R  G+L+N  EGEKY +
Sbjct: 1  MGRWVRPEVYPLLGAMGFVTSMVVFQLTRNAFLNPDCRINKEHRKMGILENKDEGEKYAQ 60

Query: 64 HFLRKYVRNKTPEIMPSINSFFA 87
          H  RKY+R + P++MPS+N FF+
Sbjct: 61 HNFRKYLRTRQPQVMPSLNRFFS 83
The following BLAST results are available for this feature:
BLAST of Spo27270.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902205077|gb|KNA15084.1|1.2e-45100.hypothetical protein SOVF_1015... [more]
gi|731335322|ref|XP_010678668.1|5.0e-4193.1PREDICTED: uncharacterized pro... [more]
gi|823121374|ref|XP_012466012.1|1.1e-4094.1PREDICTED: uncharacterized pro... [more]
gi|99646724|emb|CAK22419.1|3.3e-4093.1B12D-like protein [Beta vulgar... [more]
gi|225457158|ref|XP_002283743.1|6.1e-3984.2PREDICTED: uncharacterized pro... [more]
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BLAST of Spo27270.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9R7X1_SPIOL8.1e-46100.Uncharacterized protein OS=Spi... [more]
A0A0J8CE95_BETVU3.5e-4193.1Uncharacterized protein OS=Bet... [more]
A0A0B0PNM1_GOSAR7.8e-4194.1Uncharacterized protein OS=Gos... [more]
A0A0D2N5K0_GOSRA7.8e-4194.1Uncharacterized protein OS=Gos... [more]
Q1H8M8_BETVU2.3e-4093.1B12D-like protein OS=Beta vulg... [more]
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BLAST of Spo27270.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo27270.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT3G48140.13.7e-3475.0B12D protein[more]
AT3G29970.11.9e-2249.4B12D protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010530NADH-ubiquinone reductase complex 1 MLRQ subunitPFAMPF06522B12Dcoord: 9..76
score: 8.4
NoneNo IPR availablePANTHERPTHR33417FAMILY NOT NAMEDcoord: 4..89
score: 2.2
NoneNo IPR availablePANTHERPTHR33417:SF2SUBFAMILY NOT NAMEDcoord: 4..89
score: 2.2

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0006098 pentose-phosphate shunt
biological_process GO:0006783 heme biosynthetic process
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009156 ribonucleoside monophosphate biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009165 nucleotide biosynthetic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0032259 methylation
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0044424 intracellular part
molecular_function GO:0004749 ribose phosphate diphosphokinase activity
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0016901 oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor
molecular_function GO:0048038 quinone binding
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor
molecular_function GO:0016301 kinase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0019904 protein domain specific binding
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0038023 signaling receptor activity
molecular_function GO:0005515 protein binding
molecular_function GO:0008168 methyltransferase activity
RNA-Seq Expression