Spo08120 (gene) | SpinachBase

Overview


Name	Spo08120
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Membrane steroid-binding protein 2
Location	chr5 : 11187834 .. 11191156 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTATCTGAAAGAGGGTGACTCATACAGTCATACTCCGAATTTTTACTTATTCCATTTCAGGTTAATTGCAACATTAGGTGGAGATAAAGTTGAAGGAAAAATTTTTATCAAAAAAAAAAGCAAAAACAAAAAGTTGAAGGAAAATTTTAAAATTGTGAACTGATGAAGTGGTTTTTTAACTATGAATGTTTAGTAGATTTTTTAACAAATTGGGTTTTTTAAACTTTTAGGGCGTGTTCGGCGGCAGCTTTTGAGGTAGCGGGAAGCGTTTTGATCTAGGGCGTGTTCGACGATAGCTTTTGAGGTAGCGGGTAGCGTTTTGATCTAGGGCGTGTTTAGCGGTAGCTTTTGAGGTAGCGGGTAGCGTTTTGACCTAGGGCGTGTTCGGCGGTAACTTTTGAGGTAGCGGGTAACGGTTTGACCTATTCAAGACGCTACTTGTAAAATTTGTGAGTGTTTGGCAAGGTAGCGATTTAGGTAGCGGTTAATGGTAGAGGTAGCTTTTGTCAATCGTTAAAATTAGTACTCGGGTTTCACAAATTTAAGTAAAGTTTTAAACAATATTCTAACTTTTATTTTATTTTACAATCACAAATTCTTATTTACAATGGGTGTACAATAAATATTGTACACCAGAGTAAAAGTTAACTCAAAATGCTTAAAAGTTAAGCATACATATGTAAACGTTATCTATTTTTCAATGATAAATTTTTTCATTTTAGTAAAACTTATTTCTTCAAAATAACTAATAATGTATAAAATTAATCATTTAACCCTTTAAAATATTTATCTATCAATTTTTTTTACTAATATAAAAGTTAATCAAAACTAGGTTAAAGTTATAAAAAAAAGGTTAAAGTTATCTTGGTGTACAATAAATTTATTGTACACCTTGTGCGCACAAGATCTTTTGTTTTACAATATCAACCGCTACTTTTATCAAACACTAATATTTTACCCGCTACTTTAATAGTTATCCGTTACCCACTACTATTCACCGCTACTCGGCACTCGCTACTCTAACCACTACTCGCTTATTTTTCCAAACAAGGCGTTATTAAGTAGGAAAAGGGATTGAATGTTAATTACTTCCTCCGTAGTTTATTGAGTGGTGAGTTGATTTTATTAAACAAAGAAATAAGTGGGGTTAGTGTAGATTAAACAATTGGAGAAAAAATAGTGAAAGTATTAAAATTTATTTTTATTTTTTTGAGGGGAAGGTGAAAGTATTAAATATGACAATTCTCATTAACCTATAATAAATACGGAAAAATACAGTAATGTTGTAATGTGAATCTTGTTAGATTCATTTCAATATATTTTCGTATATTTTGGAATAATATACTTGGTACGGAACTACGGACTATATTTCAATGTAATCCAAAAAAACACACACCAAATGAGACAAAAAGAAAGGTACGAAGGAACTCAGGAAGTATTCACTTACCTAACTTACGATCGTGCTGAGATTATTAGAAGGGAATAAACATCAGAAAAAACGTTTGTTGTCCTCTCGATCTTCTCTGCCAGGTAACAATTGTTTCAATATTTCATGACTTGGGTTAATTTTTCTTCATTTCCATTCTTTCGAATTTACTTACATTTGGGTAATTAGGGTTTATATTTGATCTAATATTGTTGAATTTCTGCTAAAATTCCAATCTTTCTTCTTAGTGCTTGCTCCACATAGCCATTTAGCTTATACTTGAAATTATCTATAACTGTTAATTTTTCTGTATAATAGTTTTCTTGCTTGGAAACTAGATTGCCTCTTTAGGTTTTATCATCCGAAAACTCCAAATTCTAGGTACTAAGGTCTATTAGTGTTGATTTTTAAGTCAAAGTTGTGATATTTGACAGATTTGAGGGATCCAATTAAACAAACAAAAATGGGGGTAATTTCCGAGAAGATAGTGGAAGTGATTTCAAAATACACAGGGCTATCACCAATGTGGTTCATGATCATGGTGGGGTTGACTATAATAAGTTACAAGATAGTTAGCGGCATGTTTGTGGCGGTGAATGATTACAAAGCAGTTGAAAAGATGAAGGAAATATTCGAGGAGGATATGAGGAGGGAGGTGGTGGAGCTGGGTCAGCTGACGGAGGAGCAGCTCAAGGAATACGACGGGTCAGACCCGAAGAAGCCTCTATTGATGGCAATTAAGGGTAATATATATGATGTTTCAAGGGGTAGGATATTTTACGGGCCTGGCGGGTCATACGCTATGTTTGCTGGGAGGGATGCTACTCGGGCTTTGGCCCTCATGTCTTTTGATCCTCAGGATCTTACTGCCAACACCGACGGTCTCAGTGAATCTGAGCTTGATGTTCTTCAAGATTGGGAGCTTAAGTTTCAAGAGAAATACCCCAAGGTCGGTGTTCTTGGAGGCCAGCCGCAGCTGCAGGAGGAGGTTGTAGAAACTAAAAAAGCTGTATAGCTTACTCTTTCTAATTTCTAATTTTCTTTGTTTCATGTTTGATGATATCTCTTAATGTCAGAGAGGACCTATTATTAATCAAGTAGCTTTTGATGATATATTGATCGAACTATGTCTTATTAATCTTTCATTGTAGCTTGTTGTGGTTGACATTGCACTGATTAGCACCAACTTGTGGTTTTGAGTTTATTTGATTCTGAGTTACTCAAACTAGTCATTGCACTGATTAGCACTACCAAAATGTGATTTAGTAGTTATGTGTATATTAAGGCAACTTTGTGCGATTGGTTGAGAAGTGGACAACTTTGTGTTATTTTTGTCATATTGACAAGTTTGTGTCAATGATTGCAAATGGCCCAAAGTACATGTCCATTGATGTAATTTTCTCATAACTCCCATACTGAAGTATGCATTATAAATTGGATGTCAGTATGTCAGACACAGAGTATTATACATTTTCCTTTGTTTTTACTTTTCTCTTTTCAGCAAAATGCTTATCATTTTAGCTATTATTGTGATTTGATAATCTGCCTGGCCTTCCCAATATTTGTGGAAATTAATATAAATCTTGACTACTGTGAACATCTAAAACAGCTAGTGGTGGCCTTGATCTTAGTCGTAGGTGCTCGTTCTATTTTGATTTGAACGGGCCGAATGTTATGACTTTTAATGTGGGTCTTTTTTATGAATAAGTAGAAATCAACTTTGGTAGGGATACTAAAGAACCTTGAAGAGATTTGATCTTTTCTAGTTTGTATTGGCTGGTAAGCATTATGGTGGAAAAGTTGTGGCCTGCCCTTCAATCCTGCAAGCATCAGGTATGATTTTATTTCCTTTGTGATCCGCTCATCAATGGCAGAGTCAGATTATTAGAAAC

mRNA sequence

ATGGGGTATCTGAAAGAGGAAGGGAATAAACATCAGAAAAAACGTTTGTTGTCCTCTCGATCTTCTCTGCCAGATTTGAGGGATCCAATTAAACAAACAAAAATGGGGGTAATTTCCGAGAAGATAGTGGAAGTGATTTCAAAATACACAGGGCTATCACCAATGTGGTTCATGATCATGGTGGGGTTGACTATAATAAGTTACAAGATAGTTAGCGGCATGTTTGTGGCGGTGAATGATTACAAAGCAGTTGAAAAGATGAAGGAAATATTCGAGGAGGATATGAGGAGGGAGGTGGTGGAGCTGGGTCAGCTGACGGAGGAGCAGCTCAAGGAATACGACGGGTCAGACCCGAAGAAGCCTCTATTGATGGCAATTAAGGGTAATATATATGATGTTTCAAGGGGTAGGATATTTTACGGGCCTGGCGGGTCATACGCTATGTTTGCTGGGAGGGATGCTACTCGGGCTTTGGCCCTCATGTCTTTTGATCCTCAGGATCTTACTGCCAACACCGACGGTCTCAGTGAATCTGAGCTTGATGTTCTTCAAGATTGGGAGCTTAAGTTTCAAGAGAAATACCCCAAGGTCGGTGTTCTTGGAGGCCAGCCGCAGCTGCAGGAGGAGGTTGTAGAAACTAAAAAAGCTGTATAGCTTACTCTTTCTAATTTCTAATTTTCTTTGTTTCATGTTTGATGATATCTCTTAATGTCAGAGAGGACCTATTATTAATCAAGTAGCTTTTGATGATATATTGATCGAACTATGTCTTATTAATCTTTCATTGTAGCTTGTTGTGGTTGACATTGCACTGATTAGCACCAACTTGTGGTTTTGAGTTTATTTGATTCTGAGTTACTCAAACTAGTCATTGCACTGATTAGCACTACCAAAATGTGATTTAGTAGTTATGTGTATATTAAGGCAACTTTGTGCGATTGGTTGAGAAGTGGACAACTTTGTGTTATTTTTGTCATATTGACAAGTTTGTGTCAATGATTGCAAATGGCCCAAAGTACATGTCCATTGATGTAATTTTCTCATAACTCCCATACTGAAGTATGCATTATAAATTGGATGTCAGTATGTCAGACACAGAGTATTATACATTTTCCTTTGTTTTTACTTTTCTCTTTTCAGCAAAATGCTTATCATTTTAGCTATTATTGTGATTTGATAATCTGCCTGGCCTTCCCAATATTTGTGGAAATTAATATAAATCTTGACTACTGTGAACATCTAAAACAGCTAGTGGTGGCCTTGATCTTAGTCGTAGGTGCTCGTTCTATTTTGATTTGAACGGGCCGAATGTTATGACTTTTAATGTGGGTCTTTTTTATGAATAAGTAGAAATCAACTTTGGTAGGGATACTAAAGAACCTTGAAGAGATTTGATCTTTTCTAGTTTGTATTGGCTGGTAAGCATTATGGTGGAAAAGTTGTGGCCTGCCCTTCAATCCTGCAAGCATCAGGTATGATTTTATTTCCTTTGTGATCCGCTCATCAATGGCAGAGTCAGATTATTAGAAAC

Coding sequence (CDS)

ATGGGGTATCTGAAAGAGGAAGGGAATAAACATCAGAAAAAACGTTTGTTGTCCTCTCGATCTTCTCTGCCAGATTTGAGGGATCCAATTAAACAAACAAAAATGGGGGTAATTTCCGAGAAGATAGTGGAAGTGATTTCAAAATACACAGGGCTATCACCAATGTGGTTCATGATCATGGTGGGGTTGACTATAATAAGTTACAAGATAGTTAGCGGCATGTTTGTGGCGGTGAATGATTACAAAGCAGTTGAAAAGATGAAGGAAATATTCGAGGAGGATATGAGGAGGGAGGTGGTGGAGCTGGGTCAGCTGACGGAGGAGCAGCTCAAGGAATACGACGGGTCAGACCCGAAGAAGCCTCTATTGATGGCAATTAAGGGTAATATATATGATGTTTCAAGGGGTAGGATATTTTACGGGCCTGGCGGGTCATACGCTATGTTTGCTGGGAGGGATGCTACTCGGGCTTTGGCCCTCATGTCTTTTGATCCTCAGGATCTTACTGCCAACACCGACGGTCTCAGTGAATCTGAGCTTGATGTTCTTCAAGATTGGGAGCTTAAGTTTCAAGAGAAATACCCCAAGGTCGGTGTTCTTGGAGGCCAGCCGCAGCTGCAGGAGGAGGTTGTAGAAACTAAAAAAGCTGTATAG

Protein sequence

MGYLKEEGNKHQKKRLLSSRSSLPDLRDPIKQTKMGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETKKAV

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo08120.1	Spo08120.1	mRNA

Homology

[Close]

BLAST of Spo08120.1 vs. NCBI nr
Match: gi|902106558|gb|KNA03627.1| (hypothetical protein SOVF_207300 [Spinacia oleracea])

HSP 1 Score: 356.3 bits (913), Expect = 3.800e-95
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1

		  
Query: 35  MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 94
           MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED
Sbjct: 1   MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 60

Query: 95  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 154
           MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA
Sbjct: 61  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 120

Query: 155 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 214
           TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK
Sbjct: 121 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 180

Query: 215 KAV 218
           KAV
Sbjct: 181 KAV 183

[Close]

BLAST of Spo08120.1 vs. NCBI nr
Match: gi|902191300|gb|KNA11814.1| (hypothetical protein SOVF_131630 [Spinacia oleracea])

HSP 1 Score: 255.4 bits (651), Expect = 9.100e-65
Identity = 131/144 (90.97%), Postives = 135/144 (93.75%), Query Frame = 1

		  
Query: 74  MFVAVNDYKAVEKMKEIFEEDMRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDV 133
           MFVAVNDYKAVEKMKEIFEEDMRRE VELGQLTEEQL+EYDG DPKKPLLMA+KGNIYDV
Sbjct: 1   MFVAVNDYKAVEKMKEIFEEDMRREAVELGQLTEEQLREYDGPDPKKPLLMAMKGNIYDV 60

Query: 134 SRGRIFYGPGGSYAMFAGRDATRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEK 193
           SRGRIFYGPGGSYAMF GRDATRALALMSFDPQDLTANTDGLSESELDVLQ+WELKFQE 
Sbjct: 61  SRGRIFYGPGGSYAMFDGRDATRALALMSFDPQDLTANTDGLSESELDVLQNWELKFQEN 120

Query: 194 YPKVGVLGGQPQLQEEVVETKKAV 218
            PK+GV GGQ QLQEEVVETK  V
Sbjct: 121 -PKIGVHGGQQQLQEEVVETKNVV 143

[Close]

BLAST of Spo08120.1 vs. NCBI nr
Match: gi|731316408|ref|XP_010695409.1| (PREDICTED: probable steroid-binding protein 3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 242.7 bits (618), Expect = 6.100e-61
Identity = 130/182 (71.43%), Postives = 149/182 (81.87%), Query Frame = 1

		  
Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           IVE I  YTGLSP+   I+  LTII+YKIV  MFVAVNDYK VE+MKEI+E+DM+RE V+
Sbjct: 12  IVEAIKNYTGLSPLSLTIITVLTIITYKIVCTMFVAVNDYKEVEEMKEIYEQDMKREPVQ 71

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG++TEE+L++YDGSD KKPLLMAIKG I+DVSRGR+FYG  G Y+MFAGRDATRALALM
Sbjct: 72  LGEVTEEELRKYDGSDLKKPLLMAIKGQIFDVSRGRMFYGRDGPYSMFAGRDATRALALM 131

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQE------EVVETKK 218
           SFD QDLT N DGLSESELDVLQDWELKF EKYPKVGVL  +    E      E++ETKK
Sbjct: 132 SFDVQDLTGNIDGLSESELDVLQDWELKFLEKYPKVGVLVYKENSAEAETETDELLETKK 191

[Close]

BLAST of Spo08120.1 vs. NCBI nr
Match: gi|747048057|ref|XP_011070022.1| (PREDICTED: membrane steroid-binding protein 2-like [Sesamum indicum])

HSP 1 Score: 203.4 bits (516), Expect = 4.100e-49
Identity = 103/161 (63.98%), Postives = 124/161 (77.02%), Query Frame = 1

		  
Query: 40  EKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREV 99
           E + E I+ YTGLSP  F  ++ L  ++YK+V GMFVA +DY AV++  E       RE 
Sbjct: 5   ETLTESINHYTGLSPAAFFTILALMYVTYKVVCGMFVAADDYAAVKRANEF----ALREP 64

Query: 100 VELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALA 159
           V++G +TEE+L+ YDGSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALA
Sbjct: 65  VQMGDVTEEELRAYDGSDPSKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALA 124

Query: 160 LMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL 201
           LMSFDP+DLT N +GLS SEL+VLQDWE KF EKY KVG L
Sbjct: 125 LMSFDPKDLTGNIEGLSASELEVLQDWEYKFMEKYVKVGQL 161

[Close]

BLAST of Spo08120.1 vs. NCBI nr
Match: gi|1021034218|gb|KZM92002.1| (hypothetical protein DCAR_020633 [Daucus carota subsp. sativus])

HSP 1 Score: 194.9 bits (494), Expect = 1.500e-46
Identity = 100/174 (57.47%), Postives = 125/174 (71.84%), Query Frame = 1

		  
Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           + + IS YTGLSP  F  ++ L  + YK+V GMFVA +DY+AV++  +     + RE V+
Sbjct: 7   VTDTISYYTGLSPAAFFTILALMYVVYKVVCGMFVAADDYRAVKRSNDF----VLREPVQ 66

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG +T E+L+ YDGSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALALM
Sbjct: 67  LGDVTVEELRAYDGSDPNKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALALM 126

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETKK 216
           SF+ +DLT N DGLS  EL+VLQDWE KF EKY KVG +  +    E   +  K
Sbjct: 127 SFESKDLTGNIDGLSPDELEVLQDWEYKFMEKYMKVGQIVSEKSKAEHTEDEDK 176

[Close]

BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q8N3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_207300 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.600e-95
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1

		  
Query: 35  MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 94
           MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED
Sbjct: 1   MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 60

Query: 95  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 154
           MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA
Sbjct: 61  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 120

Query: 155 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 214
           TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK
Sbjct: 121 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 180

Query: 215 KAV 218
           KAV
Sbjct: 181 KAV 183

[Close]

BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QX08_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_131630 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.400e-65
Identity = 131/144 (90.97%), Postives = 135/144 (93.75%), Query Frame = 1

		  
Query: 74  MFVAVNDYKAVEKMKEIFEEDMRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDV 133
           MFVAVNDYKAVEKMKEIFEEDMRRE VELGQLTEEQL+EYDG DPKKPLLMA+KGNIYDV
Sbjct: 1   MFVAVNDYKAVEKMKEIFEEDMRREAVELGQLTEEQLREYDGPDPKKPLLMAMKGNIYDV 60

Query: 134 SRGRIFYGPGGSYAMFAGRDATRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEK 193
           SRGRIFYGPGGSYAMF GRDATRALALMSFDPQDLTANTDGLSESELDVLQ+WELKFQE 
Sbjct: 61  SRGRIFYGPGGSYAMFDGRDATRALALMSFDPQDLTANTDGLSESELDVLQNWELKFQEN 120

Query: 194 YPKVGVLGGQPQLQEEVVETKKAV 218
            PK+GV GGQ QLQEEVVETK  V
Sbjct: 121 -PKIGVHGGQQQLQEEVVETKNVV 143

[Close]

BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CZ14_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g029570 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.300e-61
Identity = 130/182 (71.43%), Postives = 149/182 (81.87%), Query Frame = 1

		  
Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           IVE I  YTGLSP+   I+  LTII+YKIV  MFVAVNDYK VE+MKEI+E+DM+RE V+
Sbjct: 12  IVEAIKNYTGLSPLSLTIITVLTIITYKIVCTMFVAVNDYKEVEEMKEIYEQDMKREPVQ 71

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG++TEE+L++YDGSD KKPLLMAIKG I+DVSRGR+FYG  G Y+MFAGRDATRALALM
Sbjct: 72  LGEVTEEELRKYDGSDLKKPLLMAIKGQIFDVSRGRMFYGRDGPYSMFAGRDATRALALM 131

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQE------EVVETKK 218
           SFD QDLT N DGLSESELDVLQDWELKF EKYPKVGVL  +    E      E++ETKK
Sbjct: 132 SFDVQDLTGNIDGLSESELDVLQDWELKFLEKYPKVGVLVYKENSAEAETETDELLETKK 191

[Close]

BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A161ZVE4_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_020633 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.000e-46
Identity = 100/174 (57.47%), Postives = 125/174 (71.84%), Query Frame = 1

		  
Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           + + IS YTGLSP  F  ++ L  + YK+V GMFVA +DY+AV++  +     + RE V+
Sbjct: 7   VTDTISYYTGLSPAAFFTILALMYVVYKVVCGMFVAADDYRAVKRSNDF----VLREPVQ 66

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG +T E+L+ YDGSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALALM
Sbjct: 67  LGDVTVEELRAYDGSDPNKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALALM 126

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETKK 216
           SF+ +DLT N DGLS  EL+VLQDWE KF EKY KVG +  +    E   +  K
Sbjct: 127 SFESKDLTGNIDGLSPDELEVLQDWEYKFMEKYMKVGQIVSEKSKAEHTEDEDK 176

[Close]

BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A103XQH7_CYNCS (Cytochrome b5-like heme/steroid binding domain-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_002882 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 8.600e-46
Identity = 94/159 (59.12%), Postives = 123/159 (77.36%), Query Frame = 1

		  
Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           + + I++YTGLSP  F  +  + +++YK+V+ MFVA +DY AV++   I      RE V+
Sbjct: 7   VTDAINQYTGLSPAAFFTISAMMVVAYKVVTTMFVAADDYVAVKREHNI----SLREPVQ 66

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           +G +TEE+L+ Y+GSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALAL+
Sbjct: 67  VGSITEEELRPYNGSDPNKPLLMAIKGKIYDVSRSRMFYGPGGPYALFAGRDASRALALL 126

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL 201
           SF+P DLT N DGL  S+L+VL+DWE KF+EKY KVG L
Sbjct: 127 SFEPSDLTGNIDGLGASDLEVLEDWEAKFEEKYVKVGQL 161

[Close]

BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: MSBP2_ARATH (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.800e-40
Identity = 92/182 (50.55%), Postives = 120/182 (65.93%), Query Frame = 1

		  
Query: 38  ISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRR 97
           I E + E I+ YTGLSP  F  ++ L    Y++VSG FV+   ++    ++   + +   
Sbjct: 5   IWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRP-RSLEVQPQSEPLP 64

Query: 98  EVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRA 157
             V+LG++TEE+LK YDGSD KKPLLMAIKG IYDVS+ R+FYGPGG YA+FAG+DA+RA
Sbjct: 65  PPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRA 124

Query: 158 LALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL---GGQPQLQEEVVETK 217
           LA MSF+ QDLT +  GL   EL+ LQDWE KF  KY KVG +    G+ +   E  E K
Sbjct: 125 LAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPSEAK 184

[Close]

BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: MSBP1_ARATH (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 163.3 bits (412), Expect = 3.000e-39
Identity = 87/173 (50.29%), Postives = 118/173 (68.21%), Query Frame = 1

		  
Query: 40  EKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVA----VNDYKAVEKMKEIFEEDM 99
           + + E I  YTGLSP+ F   + L    Y+++SG F +    VN ++    + +  EE  
Sbjct: 7   QTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQE-EEPP 66

Query: 100 RREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDAT 159
             + V++G++TEE+LK+YDGSDP+KPLLMAIK  IYDV++ R+FYGPGG YA+FAG+DA+
Sbjct: 67  IPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDAS 126

Query: 160 RALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG---VLGGQPQ 206
           RALA MSF+ +DLT +  GL   ELD LQDWE KF  KY KVG   V G +P+
Sbjct: 127 RALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPE 178

[Close]

BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: SBP3_ARATH (Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.100e-24
Identity = 49/95 (51.58%), Postives = 72/95 (75.79%), Query Frame = 1

		  
Query: 104 QLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSF 163
           + T EQL +Y+G+D  KP+ +AIKG ++DV+ G+ FYG GG Y+MFAG+DA+RAL  MS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 164 DPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG 199
           + +D++ + +GL+E E++ L DWE KF+ KYP VG
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVG 96

[Close]

BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: NEUFC_CAEBR (Neuferricin homolog OS=Caenorhabditis briggsae GN=tag-131 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.200e-17
Identity = 52/120 (43.33%), Postives = 68/120 (56.67%), Query Frame = 1

		  
Query: 106 TEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDP 165
           T EQL  +DGS   KP  +AI G +YDV   + +YGPG SY  FAGRDATRA     F  
Sbjct: 102 TPEQLHFFDGSRDSKPCYLAILGRVYDVDGKKEYYGPGKSYHHFAGRDATRAFTTGDFTE 161

Query: 166 QDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLG-------GQP--QLQEEVVETKKA 217
             L A+T GLS  EL  ++DW   + ++YP VGV+        GQP  +L + +   +KA
Sbjct: 162 NGLVASTHGLSHDELLSIRDWVSFYDKEYPLVGVVADLYYDSDGQPTAELTDVLARVEKA 221

[Close]

BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: NENF_BOVIN (Neudesin OS=Bos taurus GN=NENF PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.200e-16
Identity = 43/94 (45.74%), Postives = 61/94 (64.89%), Query Frame = 1

		  
Query: 106 TEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDP 165
           TEE+L  Y G +  +P+ MA+KG ++DV+ G+ FYG G  Y    G+D+TR +A MS DP
Sbjct: 45  TEEELARYGGEEEDQPIYMAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDP 104

Query: 166 QDLTANTDGLSESELDVLQD-WELKFQEKYPKVG 199
            DLT +T GL+  EL+ L D +   ++ KYP VG
Sbjct: 105 ADLTHDTTGLTAEELESLDDVFTRVYKAKYPIVG 138

[Close]

BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3)

HSP 1 Score: 165.2 bits (417), Expect = 4.400e-41
Identity = 92/182 (50.55%), Postives = 120/182 (65.93%), Query Frame = 1

		  
Query: 38  ISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRR 97
           I E + E I+ YTGLSP  F  ++ L    Y++VSG FV+   ++    ++   + +   
Sbjct: 5   IWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRP-RSLEVQPQSEPLP 64

Query: 98  EVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRA 157
             V+LG++TEE+LK YDGSD KKPLLMAIKG IYDVS+ R+FYGPGG YA+FAG+DA+RA
Sbjct: 65  PPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRA 124

Query: 158 LALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL---GGQPQLQEEVVETK 217
           LA MSF+ QDLT +  GL   EL+ LQDWE KF  KY KVG +    G+ +   E  E K
Sbjct: 125 LAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPSEAK 184

[Close]

BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT5G52240.1 (membrane steroid binding protein 1)

HSP 1 Score: 163.3 bits (412), Expect = 1.700e-40
Identity = 87/173 (50.29%), Postives = 118/173 (68.21%), Query Frame = 1

		  
Query: 40  EKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVA----VNDYKAVEKMKEIFEEDM 99
           + + E I  YTGLSP+ F   + L    Y+++SG F +    VN ++    + +  EE  
Sbjct: 7   QTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQE-EEPP 66

Query: 100 RREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDAT 159
             + V++G++TEE+LK+YDGSDP+KPLLMAIK  IYDV++ R+FYGPGG YA+FAG+DA+
Sbjct: 67  IPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDAS 126

Query: 160 RALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG---VLGGQPQ 206
           RALA MSF+ +DLT +  GL   ELD LQDWE KF  KY KVG   V G +P+
Sbjct: 127 RALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPE 178

[Close]

BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2)

HSP 1 Score: 114.0 bits (284), Expect = 1.200e-25
Identity = 49/95 (51.58%), Postives = 72/95 (75.79%), Query Frame = 1

		  
Query: 104 QLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSF 163
           + T EQL +Y+G+D  KP+ +AIKG ++DV+ G+ FYG GG Y+MFAG+DA+RAL  MS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 164 DPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG 199
           + +D++ + +GL+E E++ L DWE KF+ KYP VG
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVG 96

[Close]

BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4)

HSP 1 Score: 82.0 bits (201), Expect = 4.900e-16
Identity = 40/96 (41.67%), Postives = 58/96 (60.42%), Query Frame = 1

		  
Query: 108 EQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDPQD 167
           E+L  Y+G+D   P+L+ I G+++DV++G+  YG GG Y  FAGRDA+RA    +F    
Sbjct: 45  EELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTGDG 104

Query: 168 LTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQ 204
           LT +  GLS SE+  + DW   +   Y  VG L G+
Sbjct: 105 LTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLVGR 140

The following BLAST results are available for this feature:

BLAST of Spo08120.1 vs. NCBI nr
BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
BLAST of Spo08120.1 vs. TAIR (Arabidopsis)

BLAST of Spo08120.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902106558\|gb\|KNA03627.1\|	3.8e-95	100.	hypothetical protein SOVF_2073...	[more]
gi\|902191300\|gb\|KNA11814.1\|	9.1e-65	90.9	hypothetical protein SOVF_1316...	[more]
gi\|731316408\|ref\|XP_010695409.1\|	6.1e-61	71.4	PREDICTED: probable steroid-bi...	[more]
gi\|747048057\|ref\|XP_011070022.1\|	4.1e-49	63.9	PREDICTED: membrane steroid-bi...	[more]
gi\|1021034218\|gb\|KZM92002.1\|	1.5e-46	57.4	hypothetical protein DCAR_0206...	[more]

BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9Q8N3_SPIOL	2.6e-95	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QX08_SPIOL	6.4e-65	90.9	Uncharacterized protein OS=Spi...	[more]
A0A0J8CZ14_BETVU	4.3e-61	71.4	Uncharacterized protein OS=Bet...	[more]
A0A161ZVE4_DAUCA	1.0e-46	57.4	Uncharacterized protein OS=Dau...	[more]
A0A103XQH7_CYNCS	8.6e-46	59.1	Cytochrome b5-like heme/steroi...	[more]

BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
MSBP2_ARATH	7.8e-40	50.5	Membrane steroid-binding prote...	[more]
MSBP1_ARATH	3.0e-39	50.2	Membrane steroid-binding prote...	[more]
SBP3_ARATH	2.1e-24	51.5	Probable steroid-binding prote...	[more]
NEUFC_CAEBR	3.2e-17	43.3	Neuferricin homolog OS=Caenorh...	[more]
NENF_BOVIN	1.2e-16	45.7	Neudesin OS=Bos taurus GN=NENF...	[more]

BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4

Position :

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Match Name	E-value	Identity	Description
AT3G48890.1	4.4e-41	50.5	membrane-associated progestero...	[more]
AT5G52240.1	1.7e-40	50.2	membrane steroid binding prote...	[more]
AT2G24940.1	1.2e-25	51.5	membrane-associated progestero...	[more]
AT4G14965.1	4.9e-16	41.6	membrane-associated progestero...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	3.10.120.10		coord: 104..200 score: 1.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 106..200 score: 2.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 105..201 score: 7.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 101..200 score: 1.13
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 35..200 score: 4.7
None	No IPR available	PANTHER	PTHR10281:SF40	SUBFAMILY NOT NAMED	coord: 35..200 score: 4.7

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation
biological_process	GO:0046777	protein autophosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
biological_process	GO:0006508	proteolysis
biological_process	GO:0006366	transcription by RNA polymerase II
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
molecular_function	GO:0020037	heme binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0005516	calmodulin binding
molecular_function	GO:0004683	calmodulin-dependent protein kinase activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0003723	RNA binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo11918	0.72	Barchart \| Table
Spo01578	0.71	Barchart \| Table
Spo01231	0.70	Barchart \| Table
Spo26394	0.69	Barchart \| Table
Spo23664	0.69	Barchart \| Table
Spo16911	0.68	Barchart \| Table
Spo21691	0.68	Barchart \| Table
Spo27004	0.67	Barchart \| Table
Spo13202	0.67	Barchart \| Table
Spo15639	0.67	Barchart \| Table
Spo18575	0.67	Barchart \| Table
Spo01184	0.67	Barchart \| Table
Spo17329	0.66	Barchart \| Table
Spo21623	0.66	Barchart \| Table
Spo05306	0.65	Barchart \| Table
Spo12176	0.65	Barchart \| Table
Spo17371	0.65	Barchart \| Table
Spo14225	0.65	Barchart \| Table
Spo19558	0.65	Barchart \| Table