Spo05480 (gene) | SpinachBase

Overview


Name	Spo05480
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Thioesterase family protein
Location	SpoScf_00642 : 155481 .. 156745 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAGCAAAACAGGTTCTACAGTTGAGCGACGACGAGTTTGGGAGCGTTTCGCGACTCGTTGTTAAGCCGCACGAGCCCCAAATAGGACCTAGTTTCTACGAGGATTTTGCCCTCAGAGGCATCCAAGTTGACCGAGTCGAACCAGGTCTGATCTTCTGCACCTTCAAGGTCCCTCCTCGCCTCATTGTAAGATTCTTTCTAAACTCATTTCACTCCCTTTTCTTATTTTACGGAATTACAGTGATTTATTTGATTGCTTCCTGTATAATTTTTGACCTAATTATGCTTATGATTCTATCGTAATTTTGAGGCAGCTGTGAAATTAAATTATTGGAATTTATAATAATCAAAAGAAAGGGTGTCAATTTGTGCTTAAAATTAACCCAGAAATTATACTATTGGGAATTTTTATACTCCGTAATCAAATAGAAAGGTGTTTTTTTTTCCTATTGGTGTCGAAATGAGATGGGGAAATGATTTCTTAGCTCTATCCGTAATCCTTAAGCTTGAGTTTGTGGTTGTATTGGAACTCTGTTGAAATTTGCTTAGAATTTGGGGTTTGAATAAATGCTCAATTAGTGGTTTATGATTTTCTAGTGCATATATTGGTTAAGGAAATGTGGGTCTTTGTGTGACGGATACGGATGAAGTTGGGATACGTTTGGACCTCTTGTGATTTGCCCACAGCGGATTCTGATTTTGTGATGATACTGATATAGTGATATATTCTGATAGTGATATATGATGTCTAATCCTTTGGAGCCAAAGAAATATATTTTACGTCCGTTACTGTTTTTGTTCAGCTATGATGGTGGTGGTATGTGTATATTTTGATGATTATGAGAGTGGTATGTAATACTTGAAACTTTGTAATACTTTGAATATTATCCAGAATGAAAGAGTTCTACACTTCTACAAGTGGCTGCTTCAGCTCAGGTTGAAGTATGAAATTAGAATATACCATCTCTATTTAAAAATACTCCATATTAGGCCACGTTTTAATTTTTGCTTTGACTTGTTAGTTGTTACTTGTAAGAATATATAACGATGCACTGATCTATAAGGTGAGACTTGAGAGTCATTATTTAATTGCGTAACAAAGTTTGTTAAAAGTAATTATTTTGAGACAAATGGAGTACATTTTTATAAAGCAATCATATTTTGAAGTAGAATGGTTCATTTTAAGTATGATGTTTTGAGGCTATAGGATGAGCATGAAGGCTGCGATTGGCTGCATCAGGCCATCTGCTAAGAATTAG

mRNA sequence

ATGGAGGGAGCAAAACAGGTTCTACAGTTGAGCGACGACGAGTTTGGGAGCGTTTCGCGACTCGTTGTTAAGCCGCACGAGCCCCAAATAGGACCTAGTTTCTACGAGGATTTTGCCCTCAGAGGCATCCAAGTTGACCGAGTCGAACCAGGTCTGATCTTCTGCACCTTCAAGGTCCCTCCTCGCCTCATTAATGAAAGAGTTCTACACTTCTACAAGTGGCTGCTTCAGCTCAGGTTGAAGATGAGCATGAAGGCTGCGATTGGCTGCATCAGGCCATCTGCTAAGAATTAG

Coding sequence (CDS)

ATGGAGGGAGCAAAACAGGTTCTACAGTTGAGCGACGACGAGTTTGGGAGCGTTTCGCGACTCGTTGTTAAGCCGCACGAGCCCCAAATAGGACCTAGTTTCTACGAGGATTTTGCCCTCAGAGGCATCCAAGTTGACCGAGTCGAACCAGGTCTGATCTTCTGCACCTTCAAGGTCCCTCCTCGCCTCATTAATGAAAGAGTTCTACACTTCTACAAGTGGCTGCTTCAGCTCAGGTTGAAGATGAGCATGAAGGCTGCGATTGGCTGCATCAGGCCATCTGCTAAGAATTAG

Protein sequence

MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVPPRLINERVLHFYKWLLQLRLKMSMKAAIGCIRPSAKN

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo05480.1	Spo05480.1	mRNA

Homology

[Close]

BLAST of Spo05480.1 vs. NCBI nr
Match: gi|902238470|gb|KNA25205.1| (hypothetical protein SOVF_008570 [Spinacia oleracea])

HSP 1 Score: 135.6 bits (340), Expect = 4.700e-29
Identity = 64/65 (98.46%), Postives = 65/65 (100.00%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP
Sbjct: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60

Query: 61 PRLIN 66
          PRLI+
Sbjct: 61 PRLID 65

[Close]

BLAST of Spo05480.1 vs. NCBI nr
Match: gi|731348708|ref|XP_010685625.1| (PREDICTED: putative esterase C31F10.02 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 100.9 bits (250), Expect = 1.300e-18
Identity = 49/63 (77.78%), Postives = 52/63 (82.54%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK VL LS+DE  SVSRL V PHE Q+GPSFYEDFALRGI+VDRVEP  I CTFKVP
Sbjct: 1  MERAKHVLDLSEDESESVSRLSVNPHEFQVGPSFYEDFALRGIRVDRVEPEFISCTFKVP 60

Query: 61 PRL 64
          PRL
Sbjct: 61 PRL 63

[Close]

BLAST of Spo05480.1 vs. NCBI nr
Match: gi|870853242|gb|KMT05123.1| (hypothetical protein BVRB_7g172740 isoform A [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 100.9 bits (250), Expect = 1.300e-18
Identity = 49/63 (77.78%), Postives = 52/63 (82.54%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK VL LS+DE  SVSRL V PHE Q+GPSFYEDFALRGI+VDRVEP  I CTFKVP
Sbjct: 1  MERAKHVLDLSEDESESVSRLSVNPHEFQVGPSFYEDFALRGIRVDRVEPEFISCTFKVP 60

Query: 61 PRL 64
          PRL
Sbjct: 61 PRL 63

[Close]

BLAST of Spo05480.1 vs. NCBI nr
Match: gi|645216274|ref|XP_008220367.1| (PREDICTED: acyl-coenzyme A thioesterase 13-like [Prunus mume])

HSP 1 Score: 96.3 bits (238), Expect = 3.200e-17
Identity = 45/63 (71.43%), Postives = 51/63 (80.95%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK+ L+L+DDE  +VSRL V+PH    GPSFYE FALRGIQVDRVEPGL+ CTFKVP
Sbjct: 11 MEKAKRSLELTDDESEAVSRLAVRPHRAGAGPSFYEYFALRGIQVDRVEPGLVVCTFKVP 70

Query: 61 PRL 64
          P L
Sbjct: 71 PHL 73

[Close]

BLAST of Spo05480.1 vs. NCBI nr
Match: gi|595843536|ref|XP_007208636.1| (hypothetical protein PRUPE_ppa019870mg [Prunus persica])

HSP 1 Score: 92.0 bits (227), Expect = 6.000e-16
Identity = 44/63 (69.84%), Postives = 49/63 (77.78%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK+ L+L+DDE  +VSRL V PH    GPSFYE FALRGIQVDRVEP L+ CTFKVP
Sbjct: 1  MEKAKRSLELTDDESEAVSRLAVPPHRAGAGPSFYEYFALRGIQVDRVEPRLVVCTFKVP 60

Query: 61 PRL 64
          P L
Sbjct: 61 PHL 63

[Close]

BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S0C4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_008570 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 3.300e-29
Identity = 64/65 (98.46%), Postives = 65/65 (100.00%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP
Sbjct: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60

Query: 61 PRLIN 66
          PRLI+
Sbjct: 61 PRLID 65

[Close]

BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BYU6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g172740 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.900e-19
Identity = 49/63 (77.78%), Postives = 52/63 (82.54%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK VL LS+DE  SVSRL V PHE Q+GPSFYEDFALRGI+VDRVEP  I CTFKVP
Sbjct: 1  MERAKHVLDLSEDESESVSRLSVNPHEFQVGPSFYEDFALRGIRVDRVEPEFISCTFKVP 60

Query: 61 PRL 64
          PRL
Sbjct: 61 PRL 63

[Close]

BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BYQ9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g172740 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.900e-19
Identity = 49/63 (77.78%), Postives = 52/63 (82.54%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK VL LS+DE  SVSRL V PHE Q+GPSFYEDFALRGI+VDRVEP  I CTFKVP
Sbjct: 1  MERAKHVLDLSEDESESVSRLSVNPHEFQVGPSFYEDFALRGIRVDRVEPEFISCTFKVP 60

Query: 61 PRL 64
          PRL
Sbjct: 61 PRL 63

[Close]

BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
Match: M5WSQ0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019870mg PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 4.200e-16
Identity = 44/63 (69.84%), Postives = 49/63 (77.78%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK+ L+L+DDE  +VSRL V PH    GPSFYE FALRGIQVDRVEP L+ CTFKVP
Sbjct: 1  MEKAKRSLELTDDESEAVSRLAVPPHRAGAGPSFYEYFALRGIQVDRVEPRLVVCTFKVP 60

Query: 61 PRL 64
          P L
Sbjct: 61 PHL 63

[Close]

BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BZW0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g168180 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 9.300e-16
Identity = 42/63 (66.67%), Postives = 51/63 (80.95%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          ME AK VL+LS+ E  +VSR++V PHE  +  SFYE FA+RGI+VDRV+PGLI CTFKVP
Sbjct: 1  METAKHVLELSEKELENVSRVIVNPHECHVESSFYEIFAIRGIRVDRVQPGLISCTFKVP 60

Query: 61 PRL 64
          PRL
Sbjct: 61 PRL 63

[Close]

BLAST of Spo05480.1 vs. TAIR (Arabidopsis)
Match: AT2G29590.1 (Thioesterase superfamily protein)

HSP 1 Score: 58.9 bits (141), Expect = 2.000e-9
Identity = 34/65 (52.31%), Postives = 43/65 (66.15%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDD--EFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFK 60
          ME   + LQLSD+  + G   R+     E   G SFYE+F+LRGI+V+RVEPG I C+FK
Sbjct: 2  MEKIMEYLQLSDEVVDDGEAPRVEEFLGE---GKSFYENFSLRGIRVNRVEPGFISCSFK 61

Query: 61 VPPRL 64
          VP RL
Sbjct: 62 VPLRL 63

[Close]

BLAST of Spo05480.1 vs. TAIR (Arabidopsis)
Match: AT1G04290.1 (Thioesterase superfamily protein)

HSP 1 Score: 50.1 bits (118), Expect = 9.200e-7
Identity = 23/65 (35.38%), Postives = 34/65 (52.31%), Query Frame = 1


		  
Query: 1  MEGAKQVLQLSDDEFGSVSRLVVKPHEPQIGPSFYEDFALRGIQVDRVEPGLIFCTFKVP 60
          +E  K+ L+  +DE      +   PH       F E F   G++VD +EPG I C+ K+P
Sbjct: 3  LESVKKYLEGDEDEKAKEPMVAKLPHR------FLERFVTNGLKVDLIEPGRIVCSMKIP 61

Query: 61 PRLIN 66
          P L+N
Sbjct: 63 PHLLN 61

The following BLAST results are available for this feature:

BLAST of Spo05480.1 vs. NCBI nr
BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
BLAST of Spo05480.1 vs. ExPASy Swiss-Prot
BLAST of Spo05480.1 vs. TAIR (Arabidopsis)

BLAST of Spo05480.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902238470\|gb\|KNA25205.1\|	4.7e-29	98.4	hypothetical protein SOVF_0085...	[more]
gi\|731348708\|ref\|XP_010685625.1\|	1.3e-18	77.7	PREDICTED: putative esterase C...	[more]
gi\|870853242\|gb\|KMT05123.1\|	1.3e-18	77.7	hypothetical protein BVRB_7g17...	[more]
gi\|645216274\|ref\|XP_008220367.1\|	3.2e-17	71.4	PREDICTED: acyl-coenzyme A thi...	[more]
gi\|595843536\|ref\|XP_007208636.1\|	6.0e-16	69.8	hypothetical protein PRUPE_ppa...	[more]

BLAST of Spo05480.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9S0C4_SPIOL	3.3e-29	98.4	Uncharacterized protein OS=Spi...	[more]
A0A0J8BYU6_BETVU	8.9e-19	77.7	Uncharacterized protein OS=Bet...	[more]
A0A0J8BYQ9_BETVU	8.9e-19	77.7	Uncharacterized protein OS=Bet...	[more]
M5WSQ0_PRUPE	4.2e-16	69.8	Uncharacterized protein OS=Pru...	[more]
A0A0J8BZW0_BETVU	9.3e-16	66.6	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo05480.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo05480.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G29590.1	2.0e-9	52.3	Thioesterase superfamily prote...	[more]
AT1G04290.1	9.2e-7	35.3	Thioesterase superfamily prote...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR21660	THIOESTERASE SUPERFAMILY MEMBER-RELATED	coord: 25..66 score: 1.8
None	No IPR available	PANTHER	PTHR21660:SF8	SUBFAMILY NOT NAMED	coord: 25..66 score: 1.8

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030003	cellular cation homeostasis
biological_process	GO:0019344	cysteine biosynthetic process
biological_process	GO:0070838	divalent metal ion transport
biological_process	GO:0009773	photosynthetic electron transport in photosystem I
biological_process	GO:0009780	photosynthetic NADP+ reduction
biological_process	GO:0042550	photosystem I stabilization
biological_process	GO:0050821	protein stabilization
cellular_component	GO:0005777	peroxisome
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0030093	chloroplast photosystem I
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016168	chlorophyll binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo03279	0.77	Barchart \| Table
Spo03847	0.72	Barchart \| Table
Spo15340	0.65	Barchart \| Table