Spo06391.1 (mRNA) | SpinachBase

Overview


Name	Spo06391.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Tonoplast intrinsic protein
Location	SpoScf_00953 : 190642 .. 191617 (-)
Sequence length	432

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGTTCACCGGCTCGCGAGAAAGAGTAACAGCGGCGCTGGAGGTTGGGGCTGGCGGCGCTGGGGTACCGTGCGCGGCTGGGCTGGGTGGTCGTGCAATCTGGCCGAGTCGGGGGGTTAGGGGGTGGGGGCAACCGACTGCGTCGGGGGAGGAGGGGAGGTTCACCGGCTGCGTCGGGGGAGGGGGGTGGTTCACCGGCTGCGTCGGGGGAGGGGGGGTGGCTCTTCAACTTTCTATTTTTAATTTCTCTAAATTGGTCGAAGGAGGAGGAAGGCAGACAAGCTTTTTTTCTTCTTTTAATTTAGTGACCGTTTTTTGGTCATTGATTATTTAATTTTTTTTTAACTTTTTTTTAAAGTTTAGCGACCGCTTTTTTGGTCGGACATATTATATAGGCGGTTATTAAAATTTTAAATTTATTTTGAAAATAGTCGGAATTTACCGACTGCTCATTAGTCGGAATGGAAGTTTATGCAGTCGATAAATTTACGACTGTTATGAAAGCGGTTGGTAAATTTTAAATTTACCAACCGCTCTACATTCGGTCGGTAAATTTAGCGACCGTTTCTGATTCGGTTGGTAAATTTATTTTTACTGACTACTTTCACGTCGGTCGGTAAAATCAGTTGGTAATCAAGCGCTTTCTTGTAGTGATAGTCATGACCTTCGGACTAGTGTACACCGTTTACGCCACCGCAGTCGACCCTAAGAAGGGTGACATTGGTACCATTGCTCCCTTAGCAATTGGTCTCATTGTTGCTGCCAACATCCTTGTCGGTGGTGCCTTCACTGGTGCATCAATGAACCCCGCCGTGTCCTTCGGCCCAGCCTTGATTAGCTGGAACTGGAGCTCCCACTGGGTTTACTGGGCTGGACCACTTATTGGTGGTGGACTAGCTGGTCTCTTCTACGAGTTTATCTTCATTGGTGGTCAAGAGGCTCCCGCTGCTGATTACCAGAGACTCTCCGCTTAA

mRNA sequence

ATGTGGTTCACCGGCTCGCGAGAAAGAGTAACAGCGGCGCTGGAGGTTGGGGCTGGCGGCGCTGGGGTACCGTGCGCGGCTGGGCTGGGTGGTCGTGCAATCTGGCCGATGATAGTCATGACCTTCGGACTAGTGTACACCGTTTACGCCACCGCAGTCGACCCTAAGAAGGGTGACATTGGTACCATTGCTCCCTTAGCAATTGGTCTCATTGTTGCTGCCAACATCCTTGTCGGTGGTGCCTTCACTGGTGCATCAATGAACCCCGCCGTGTCCTTCGGCCCAGCCTTGATTAGCTGGAACTGGAGCTCCCACTGGGTTTACTGGGCTGGACCACTTATTGGTGGTGGACTAGCTGGTCTCTTCTACGAGTTTATCTTCATTGGTGGTCAAGAGGCTCCCGCTGCTGATTACCAGAGACTCTCCGCTTAA

Coding sequence (CDS)

ATGTGGTTCACCGGCTCGCGAGAAAGAGTAACAGCGGCGCTGGAGGTTGGGGCTGGCGGCGCTGGGGTACCGTGCGCGGCTGGGCTGGGTGGTCGTGCAATCTGGCCGATGATAGTCATGACCTTCGGACTAGTGTACACCGTTTACGCCACCGCAGTCGACCCTAAGAAGGGTGACATTGGTACCATTGCTCCCTTAGCAATTGGTCTCATTGTTGCTGCCAACATCCTTGTCGGTGGTGCCTTCACTGGTGCATCAATGAACCCCGCCGTGTCCTTCGGCCCAGCCTTGATTAGCTGGAACTGGAGCTCCCACTGGGTTTACTGGGCTGGACCACTTATTGGTGGTGGACTAGCTGGTCTCTTCTACGAGTTTATCTTCATTGGTGGTCAAGAGGCTCCCGCTGCTGATTACCAGAGACTCTCCGCTTAA

Protein sequence

MWFTGSRERVTAALEVGAGGAGVPCAAGLGGRAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo06391	Spo06391	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo06391.1	Spo06391.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06391.1.CDS.2	Spo06391.1.CDS.2	CDS
Spo06391.1.CDS.1	Spo06391.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06391.1.exon.2	Spo06391.1.exon.2	exon
Spo06391.1.exon.1	Spo06391.1.exon.1	exon

Homology

[Close]

BLAST of Spo06391.1 vs. NCBI nr
Match: gi|902081699|gb|KNA03133.1| (hypothetical protein SOVF_212060 [Spinacia oleracea])

HSP 1 Score: 216.1 bits (549), Expect = 4.000e-53
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1


		  
Query: 38  IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL 97
           IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL
Sbjct: 149 IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL 208

Query: 98  ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA 144
           ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA
Sbjct: 209 ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA 254

[Close]

BLAST of Spo06391.1 vs. NCBI nr
Match: gi|731368735|ref|XP_010696310.1| (PREDICTED: probable aquaporin TIP1-2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 184.5 bits (467), Expect = 1.300e-43
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 1


		  
Query: 12  AALEVGAGG---AGVPCAAGLGG-RAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLA 71
           A L+   GG   A   CA  +    A+   IVMTFGLVYTVYATA+DPKKG+IG IAPLA
Sbjct: 119 ALLKFATGGLETAAFACATDVSAWNALVMEIVMTFGLVYTVYATAIDPKKGNIGIIAPLA 178

Query: 72  IGLIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIF 131
           IGLIV ANIL GGAF GASMNPAVSFGPA++SWNW++HW+YW GPLIG G+AGL YEFIF
Sbjct: 179 IGLIVGANILAGGAFDGASMNPAVSFGPAVMSWNWTNHWIYWVGPLIGAGIAGLIYEFIF 238

Query: 132 IGGQEAPAADYQRLSA 144
           IG QE  +ADYQRLSA
Sbjct: 239 IGHQEPASADYQRLSA 254

[Close]

BLAST of Spo06391.1 vs. NCBI nr
Match: gi|145572859|gb|AAT65835.2| (tonoplast intrinsic protein gamma [Salicornia herbacea])

HSP 1 Score: 183.7 bits (465), Expect = 2.200e-43
Identity = 91/134 (67.91%), Postives = 104/134 (77.61%), Query Frame = 1


		  
Query: 14  LEVGAGGAGVPCAAGLGGRAIWPM----IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIG 73
           L+   GG      A   G  +W      IVMTFGLVYTVYATA+DPKKG+IG IAPLAIG
Sbjct: 121 LKFSTGGLQTSAFALCEGETVWGAFVMEIVMTFGLVYTVYATALDPKKGNIGIIAPLAIG 180

Query: 74  LIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIG 133
           LIV ANIL GGAF GASMNPAVSFGPAL+SWNW++HW+YWAGPLIG G+AGL YEFIF+G
Sbjct: 181 LIVGANILAGGAFDGASMNPAVSFGPALVSWNWANHWIYWAGPLIGAGIAGLIYEFIFMG 240

Query: 134 GQEAPAADYQRLSA 144
             ++ + DYQRLSA
Sbjct: 241 STDSTSTDYQRLSA 254

[Close]

BLAST of Spo06391.1 vs. NCBI nr
Match: gi|756179870|gb|AJK93570.1| (tonoplast intrinsic protein [Suaeda glauca])

HSP 1 Score: 177.9 bits (450), Expect = 1.200e-41
Identity = 90/133 (67.67%), Postives = 103/133 (77.44%), Query Frame = 1


		  
Query: 11  TAALEVGAGGAGVPCAAGLGGRAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGL 70
           T  +E  A      C+      A+   IVMTFGLVYTVYATA+DPKKG+IG IAPLAIGL
Sbjct: 125 TGGMETSAFALATDCSTW---NALVMEIVMTFGLVYTVYATAIDPKKGNIGIIAPLAIGL 184

Query: 71  IVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGG 130
           IVAANIL GGAF GASMNPAV+FGPAL+SWN+ +HW+YW GPL+GG +AGL YEFIFIG 
Sbjct: 185 IVAANILAGGAFDGASMNPAVAFGPALVSWNFVNHWIYWVGPLVGGAIAGLVYEFIFIGH 244

Query: 131 QEAPAADYQRLSA 144
           QE  +ADYQRL A
Sbjct: 245 QEPASADYQRLFA 254

[Close]

BLAST of Spo06391.1 vs. NCBI nr
Match: gi|1021047506|gb|KZN05286.1| (hypothetical protein DCAR_006123 [Daucus carota subsp. sativus])

HSP 1 Score: 175.6 bits (444), Expect = 6.000e-41
Identity = 88/124 (70.97%), Postives = 100/124 (80.65%), Query Frame = 1


		  
Query: 22  GVPCAA-GLGGRAIWPM----IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANI 81
           G+P +A  L G +IW      IVMTFGLVYTVYA AVDPKKGD+G IAP+AIG IV ANI
Sbjct: 127 GMPTSAFALSGVSIWSAFVFEIVMTFGLVYTVYAVAVDPKKGDLGIIAPIAIGFIVGANI 186

Query: 82  LVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGG--QEAP 139
           L GGAFTGASMNPAVSFGPAL+SW+W++HWVYWAGPL+GGGLAG+ YE IFIGG  +  P
Sbjct: 187 LAGGAFTGASMNPAVSFGPALVSWDWTNHWVYWAGPLVGGGLAGIIYELIFIGGSHEPLP 246

[Close]

BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q8W8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_212060 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.800e-53
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1


		  
Query: 38  IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL 97
           IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL
Sbjct: 149 IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL 208

Query: 98  ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA 144
           ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA
Sbjct: 209 ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAPAADYQRLSA 254

[Close]

BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E1T9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g179210 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 9.100e-44
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 1


		  
Query: 12  AALEVGAGG---AGVPCAAGLGG-RAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLA 71
           A L+   GG   A   CA  +    A+   IVMTFGLVYTVYATA+DPKKG+IG IAPLA
Sbjct: 119 ALLKFATGGLETAAFACATDVSAWNALVMEIVMTFGLVYTVYATAIDPKKGNIGIIAPLA 178

Query: 72  IGLIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIF 131
           IGLIV ANIL GGAF GASMNPAVSFGPA++SWNW++HW+YW GPLIG G+AGL YEFIF
Sbjct: 179 IGLIVGANILAGGAFDGASMNPAVSFGPAVMSWNWTNHWIYWVGPLIGAGIAGLIYEFIF 238

Query: 132 IGGQEAPAADYQRLSA 144
           IG QE  +ADYQRLSA
Sbjct: 239 IGHQEPASADYQRLSA 254

[Close]

BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
Match: Q6DW15_9CARY (Tonoplast intrinsic protein gamma OS=Salicornia herbacea PE=2 SV=2)

HSP 1 Score: 183.7 bits (465), Expect = 1.500e-43
Identity = 91/134 (67.91%), Postives = 104/134 (77.61%), Query Frame = 1


		  
Query: 14  LEVGAGGAGVPCAAGLGGRAIWPM----IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIG 73
           L+   GG      A   G  +W      IVMTFGLVYTVYATA+DPKKG+IG IAPLAIG
Sbjct: 121 LKFSTGGLQTSAFALCEGETVWGAFVMEIVMTFGLVYTVYATALDPKKGNIGIIAPLAIG 180

Query: 74  LIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIG 133
           LIV ANIL GGAF GASMNPAVSFGPAL+SWNW++HW+YWAGPLIG G+AGL YEFIF+G
Sbjct: 181 LIVGANILAGGAFDGASMNPAVSFGPALVSWNWANHWIYWAGPLIGAGIAGLIYEFIFMG 240

Query: 134 GQEAPAADYQRLSA 144
             ++ + DYQRLSA
Sbjct: 241 STDSTSTDYQRLSA 254

[Close]

BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
Match: A0A0C5AZ88_9CARY (Suaeda glauca tonoplast intrinsic protein mRNA OS=Suaeda glauca PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 8.500e-42
Identity = 90/133 (67.67%), Postives = 103/133 (77.44%), Query Frame = 1


		  
Query: 11  TAALEVGAGGAGVPCAAGLGGRAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGL 70
           T  +E  A      C+      A+   IVMTFGLVYTVYATA+DPKKG+IG IAPLAIGL
Sbjct: 125 TGGMETSAFALATDCSTW---NALVMEIVMTFGLVYTVYATAIDPKKGNIGIIAPLAIGL 184

Query: 71  IVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGG 130
           IVAANIL GGAF GASMNPAV+FGPAL+SWN+ +HW+YW GPL+GG +AGL YEFIFIG 
Sbjct: 185 IVAANILAGGAFDGASMNPAVAFGPALVSWNFVNHWIYWVGPLVGGAIAGLVYEFIFIGH 244

Query: 131 QEAPAADYQRLSA 144
           QE  +ADYQRL A
Sbjct: 245 QEPASADYQRLFA 254

[Close]

BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
Match: A0A166DHW7_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006123 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.200e-41
Identity = 88/124 (70.97%), Postives = 100/124 (80.65%), Query Frame = 1


		  
Query: 22  GVPCAA-GLGGRAIWPM----IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANI 81
           G+P +A  L G +IW      IVMTFGLVYTVYA AVDPKKGD+G IAP+AIG IV ANI
Sbjct: 127 GMPTSAFALSGVSIWSAFVFEIVMTFGLVYTVYAVAVDPKKGDLGIIAPIAIGFIVGANI 186

Query: 82  LVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGG--QEAP 139
           L GGAFTGASMNPAVSFGPAL+SW+W++HWVYWAGPL+GGGLAG+ YE IFIGG  +  P
Sbjct: 187 LAGGAFTGASMNPAVSFGPALVSWDWTNHWVYWAGPLVGGGLAGIIYELIFIGGSHEPLP 246

[Close]

BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
Match: TIP1_MEDTR (Probable aquaporin TIP-type OS=Medicago truncatula GN=AQP1 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 7.100e-42
Identity = 91/135 (67.41%), Postives = 106/135 (78.52%), Query Frame = 1


		  
Query: 10  VTAALEVGAGGAGVPC---AAGLG-GRAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAP 69
           V +AL V    + VP    + G+G G A+   IVMTFGLVYTVYATAVDPKKG+IG IAP
Sbjct: 116 VASALLVFVTASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAP 175

Query: 70  LAIGLIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEF 129
           +AIG IV ANILVGGAFTGASMNPAVSFGPA++SW+WS+HWVYWAGPLIGGG+AGL YE 
Sbjct: 176 IAIGFIVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEV 235

Query: 130 IFIGG--QEAPAADY 139
           +FI    ++ P  DY
Sbjct: 236 LFINSTHEQLPTTDY 250

[Close]

BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
Match: TIP1_MEDSA (Probable aquaporin TIP-type OS=Medicago sativa GN=MCP1 PE=2 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 9.300e-42
Identity = 91/135 (67.41%), Postives = 106/135 (78.52%), Query Frame = 1


		  
Query: 10  VTAALEVGAGGAGVPC---AAGLG-GRAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAP 69
           V +AL V    + VP    + G+G G A+   IVMTFGLVYTVYATAVDPKKG+IG IAP
Sbjct: 115 VCSALLVFVTASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAP 174

Query: 70  LAIGLIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEF 129
           +AIG IV ANILVGGAFTGASMNPAVSFGPA++SW+WS+HWVYWAGPLIGGG+AGL YE 
Sbjct: 175 IAIGFIVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEV 234

Query: 130 IFIGG--QEAPAADY 139
           +FI    ++ P  DY
Sbjct: 235 LFINSTHEQLPTTDY 249

[Close]

BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
Match: TIP11_ORYSJ (Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.300e-40
Identity = 83/117 (70.94%), Postives = 93/117 (79.49%), Query Frame = 1


		  
Query: 28  GLGGRAIWPM----IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFT 87
           GL G ++W      IVMTFGLVYTVYATAVDPKKG +GTIAP+AIG IV ANILVGGAF 
Sbjct: 134 GLTGVSVWEALVLEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGANILVGGAFD 193

Query: 88  GASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIG--GQEAPAADY 139
           GASMNPAVSFGPAL+SW+W S WVYW GPLIGGGLAG+ YE +FI    ++ P  DY
Sbjct: 194 GASMNPAVSFGPALVSWSWESQWVYWVGPLIGGGLAGVIYEVLFISHTHEQLPTTDY 250

[Close]

BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
Match: TIP12_ARATH (Aquaporin TIP1-2 OS=Arabidopsis thaliana GN=TIP1-2 PE=1 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 1.800e-40
Identity = 84/132 (63.64%), Postives = 101/132 (76.52%), Query Frame = 1


		  
Query: 14  LEVGAGGAGVPC---AAGLGG-RAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIG 73
           L    GG  +P    +AG+G   A+   IVMTFGLVYTVYATAVDPK G +GTIAP+AIG
Sbjct: 122 LSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIG 181

Query: 74  LIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFI- 133
            IV ANIL GGAF+GASMNPAV+FGPA++SW W++HWVYWAGPLIGGGLAG+ Y+F+FI 
Sbjct: 182 FIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLIGGGLAGIIYDFVFID 241

Query: 134 --GGQEAPAADY 139
               ++ P  DY
Sbjct: 242 ENAHEQLPTTDY 253

[Close]

BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
Match: TIP11_MAIZE (Aquaporin TIP1-1 OS=Zea mays GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 5.100e-40
Identity = 82/117 (70.09%), Postives = 92/117 (78.63%), Query Frame = 1


		  
Query: 28  GLGGRAIWPM----IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFT 87
           GL G ++W      IVMTFGLVYTVYATAVDPKKG +GTIAP+AIG IV ANILVGGAF 
Sbjct: 134 GLTGVSVWEALVLEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGANILVGGAFD 193

Query: 88  GASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIG--GQEAPAADY 139
           GASMNPAVSFGPAL+SW W   WVYW GPLIGGGLAG+ YE +FI    ++ P+ DY
Sbjct: 194 GASMNPAVSFGPALVSWEWGYQWVYWVGPLIGGGLAGVIYELLFISHTHEQLPSTDY 250

[Close]

BLAST of Spo06391.1 vs. TAIR (Arabidopsis)
Match: AT3G26520.1 (tonoplast intrinsic protein 2)

HSP 1 Score: 166.8 bits (421), Expect = 9.900e-42
Identity = 84/132 (63.64%), Postives = 101/132 (76.52%), Query Frame = 1


		  
Query: 14  LEVGAGGAGVPC---AAGLGG-RAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIG 73
           L    GG  +P    +AG+G   A+   IVMTFGLVYTVYATAVDPK G +GTIAP+AIG
Sbjct: 122 LSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIG 181

Query: 74  LIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFI- 133
            IV ANIL GGAF+GASMNPAV+FGPA++SW W++HWVYWAGPLIGGGLAG+ Y+F+FI 
Sbjct: 182 FIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLIGGGLAGIIYDFVFID 241

Query: 134 --GGQEAPAADY 139
               ++ P  DY
Sbjct: 242 ENAHEQLPTTDY 253

[Close]

BLAST of Spo06391.1 vs. TAIR (Arabidopsis)
Match: AT2G36830.1 (gamma tonoplast intrinsic protein)

HSP 1 Score: 163.3 bits (412), Expect = 1.100e-40
Identity = 83/131 (63.36%), Postives = 99/131 (75.57%), Query Frame = 1


		  
Query: 14  LEVGAGGAGVPC---AAGLGG-RAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIG 73
           L+   GG  VP    +AG+G   A    IVMTFGLVYTVYATA+DPK G +GTIAP+AIG
Sbjct: 121 LKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSLGTIAPIAIG 180

Query: 74  LIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIG 133
            IV ANIL GGAF+GASMNPAV+FGPA++SW W++HWVYWAGPL+GGG+AGL YE  FI 
Sbjct: 181 FIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVFFIN 240

Query: 134 --GQEAPAADY 139
              ++ P  DY
Sbjct: 241 TTHEQLPTTDY 251

[Close]

BLAST of Spo06391.1 vs. TAIR (Arabidopsis)
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3)

HSP 1 Score: 156.8 bits (395), Expect = 1.000e-38
Identity = 76/104 (73.08%), Postives = 86/104 (82.69%), Query Frame = 1


		  
Query: 38  IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL 97
           IVMTFGLVYTVYATAVDPKKGDIG IAPLAIGLIV ANILVGGAF GASMNPAVSFGPA+
Sbjct: 149 IVMTFGLVYTVYATAVDPKKGDIGIIAPLAIGLIVGANILVGGAFDGASMNPAVSFGPAV 208

Query: 98  ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGG---QEAPAADY 139
           +SW W++HWVYW GP IG  +A + Y+ IFIG    +  P+ D+
Sbjct: 209 VSWIWTNHWVYWVGPFIGAAIAAIVYDTIFIGSNGHEPLPSNDF 252

[Close]

BLAST of Spo06391.1 vs. TAIR (Arabidopsis)
Match: AT1G17810.1 (beta-tonoplast intrinsic protein)

HSP 1 Score: 143.7 bits (361), Expect = 9.000e-35
Identity = 65/97 (67.01%), Postives = 76/97 (78.35%), Query Frame = 1


		  
Query: 38  IVMTFGLVYTVYATAVDPKKGDIGTIAPLAIGLIVAANILVGGAFTGASMNPAVSFGPAL 97
           I++TF LVY VY+TA+DPK+G IG IAPLAIGLIV ANILVGG F GASMNPA +FGPAL
Sbjct: 157 IILTFALVYVVYSTAIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPAL 216

Query: 98  ISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIGGQEAP 135
           + W WS+HW+YW GP IGG LA L YE++ I     P
Sbjct: 217 VGWRWSNHWIYWVGPFIGGALAALIYEYMIIPSVNEP 253

[Close]

BLAST of Spo06391.1 vs. TAIR (Arabidopsis)
Match: AT3G16240.1 (delta tonoplast integral protein)

HSP 1 Score: 143.7 bits (361), Expect = 9.000e-35
Identity = 77/123 (62.60%), Postives = 89/123 (72.36%), Query Frame = 1


		  
Query: 14  LEVGAGGAGVPC---AAGLGG-RAIWPMIVMTFGLVYTVYATAVDPKKGDIGTIAPLAIG 73
           L+   GG  VP    AAGLG    +   I++TF LVYTVYATA DPKKG +GTIAPLAIG
Sbjct: 119 LKYVTGGLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIAPLAIG 178

Query: 74  LIVAANILVGGAFTGASMNPAVSFGPALISWNWSSHWVYWAGPLIGGGLAGLFYEFIFIG 133
           LIV ANIL  G F+G SMNPA SFGPA+ + ++S HWVYW GPLIGGGLAGL Y  +F+G
Sbjct: 179 LIVGANILAAGPFSGGSMNPARSFGPAVAAGDFSGHWVYWVGPLIGGGLAGLIYGNVFMG 238

The following BLAST results are available for this feature:

BLAST of Spo06391.1 vs. NCBI nr
BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
BLAST of Spo06391.1 vs. TAIR (Arabidopsis)

BLAST of Spo06391.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902081699\|gb\|KNA03133.1\|	4.0e-53	100.	hypothetical protein SOVF_2120...	[more]
gi\|731368735\|ref\|XP_010696310.1\|	1.3e-43	69.8	PREDICTED: probable aquaporin ...	[more]
gi\|145572859\|gb\|AAT65835.2\|	2.2e-43	67.9	tonoplast intrinsic protein ga...	[more]
gi\|756179870\|gb\|AJK93570.1\|	1.2e-41	67.6	tonoplast intrinsic protein [S...	[more]
gi\|1021047506\|gb\|KZN05286.1\|	6.0e-41	70.9	hypothetical protein DCAR_0061...	[more]

BLAST of Spo06391.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q8W8_SPIOL	2.8e-53	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8E1T9_BETVU	9.1e-44	69.8	Uncharacterized protein OS=Bet...	[more]
Q6DW15_9CARY	1.5e-43	67.9	Tonoplast intrinsic protein ga...	[more]
A0A0C5AZ88_9CARY	8.5e-42	67.6	Suaeda glauca tonoplast intrin...	[more]
A0A166DHW7_DAUCA	4.2e-41	70.9	Uncharacterized protein OS=Dau...	[more]

BLAST of Spo06391.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
TIP1_MEDTR	7.1e-42	67.4	Probable aquaporin TIP-type OS...	[more]
TIP1_MEDSA	9.3e-42	67.4	Probable aquaporin TIP-type OS...	[more]
TIP11_ORYSJ	1.3e-40	70.9	Probable aquaporin TIP1-1 OS=O...	[more]
TIP12_ARATH	1.8e-40	63.6	Aquaporin TIP1-2 OS=Arabidopsi...	[more]
TIP11_MAIZE	5.1e-40	70.0	Aquaporin TIP1-1 OS=Zea mays G...	[more]

BLAST of Spo06391.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G26520.1	9.9e-42	63.6	tonoplast intrinsic protein 2	[more]
AT2G36830.1	1.1e-40	63.3	gamma tonoplast intrinsic prot...	[more]
AT4G01470.1	1.0e-38	73.0	tonoplast intrinsic protein 1;...	[more]
AT1G17810.1	9.0e-35	67.0	beta-tonoplast intrinsic prote...	[more]
AT3G16240.1	9.0e-35	62.6	delta tonoplast integral prote...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 69..91 score: 6.5E-20coord: 36..54 score: 6.5E-20coord: 106..126 score: 6.5
IPR000425	Major intrinsic protein	PANTHER	PTHR19139	AQUAPORIN TRANSPORTER	coord: 38..132 score: 2.3
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 16..123 score: 1.5
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10		coord: 37..134 score: 4.5
IPR023271	Aquaporin-like	unknown	SSF81338	Aquaporin-like	coord: 33..135 score: 4.97
None	No IPR available	PANTHER	PTHR19139:SF52	AQUAPORIN TIP1-1	coord: 38..132 score: 2.3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005215	transporter activity