Spo25288.1 (mRNA) | SpinachBase

Overview


Name	Spo25288.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Actin depolymerizing factor 4
Location	SpoScf_02273 : 36669 .. 40151 (+)
Sequence length	1397

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATACCCCACAGAAAGAAAAGAAAAGAAAAATACACACATAGAGAAATTTAAATTAAAAAAAGAAAAGAAAAAAAAGAAATCAGTCTCCATTTTATACCTCCCTTATTTCCCATTCTCTCTCTATCTGCGTCTTTCTCTCTCCATCAAAGCCCTATATTCTCTCCATTTTCTCTCTCTCCTACTCTCACAGGTGCTGCTACCACTTCACTCAGAACAAGAACAAAAACAAAAATGGTATAATTCTTTTTTAAAAGTTTTTTTTTGTTAATTTTTAGTTTTGGATTCTAGGGTTAGTTCTTTTGATTATGATTTGTGTTTGATTGTTTTGGAATTATGGTGGGATTTTTATTTTATTTTGGTCTTTTGCTTTTCGATCTGGAAAATTGTGAACAGGACGACAGATTTAGTTTGAAAATTTTGGATTAATTTCGGGAATTTGAGCGTTTTTTCGGGGATGTTTCATGAACAATTATGCTTTTTTTGTTTTCTCCCTGATCTGAAAGTTATTTATTCTTTTTTCTTAATCTTTATGAAATTTCTGCTGCTACAGAATCGAGCTCGGGAATCAATAGCATCTTATGCGCTGGGATTTTAGAAACTCTTGTTATTTTTGGTATTTATTACGAATTTTATGAATTTGTTTCTGACTTGGATAATTTTGGAGTTAGTGGAACACAACTGAGGATCTGTAATTTGGTTGAGGAATCATGTTGGTTAGAAATGGGTAGCTGCAGTAGCTCGAATTCAGTGTTATTCTAAATAAGCTTGAGCTAATAGTATAATTTGTTTGATTGGATGTGGTGAATTTTGTTAGAAAAAAATTGTGGTGAATTTGATAACACTTCATTTGTTTCGTGCTATTGAGTATATGGTTATTGGCCAGATGCAATGATGATCGGTAATGTGTCTATGTTTGGATGTGTGGTTGAATGTCTTGAAGAAGATTTTGTGATTCGTAATAAGGAAATCCAGTGCATAGAATTAGCAAATCTTATTTTGTGGATATTGTCCATCTTGAGGACCCTAATTTTAGAATGTTGTTGATGTTAGAGAGTGAACCCAAGTGTAGACACTGCGATTTCCCGGGGATATCTTGTGTTGTGTGGGGTTTTGTTAAACACTTGTTGGAATTGTCTGTTCAATTTTTTTGACGCGTTGGAGTGTGTGAACTTGGTTCGTGGTTTTCCTGGTGTATGAGTTTGGCTGCTGATGACCTCTCTTAAGTTTACAGTATGTTTTACAATTGAGGTCCCAAATCTAGAGGGGAAAGGAGCATTTTCGTATGTGCTGTCTCCACTTCTCATTTGAGCTTCGGTTTTTATTTGTTTTGCTACCAAAGCACTTCGGAAATCAATATAAAAAAAGAACCTCAGTTCATAGAGCATTGACGCTGGGTTATGAATCTTCAAGAGTTTAATTGTTTCTTTGGTGGCATAGAACCATTTTTTTTTTTGAAGGAAATAGAACCATTTTCTTTATGCATATAGTTTCTTGTTTATTTGAGATGTTGGGTTTCCCTAATTAAGTGGAGTAAGTTCTGGATAACCATGAACTTCATTGGAAGGATTGTATTTGTGACCTTTTGAGTGGAAGAGGATGGAGAGAGCTTTAGTTTACAGAAAAGTTACTAAGTGCTGTGGTGTCAAAACTTTAACCATTACCCAGTATGCTTGGTAATAGAAGAGCTTGTAATTGTAATATTATATTATAGTTATCCAAGGCTATGAAGATGCAAGATTGAAATTTATTTCTCCTTTGATTGCGCTTCTTTTTTTAATTCAAAGTGCAGCTAAAAGCTTTTAGCTAATTTTAACCAAAAAATTTCTCATGCAATGTTTCACTTAATTATTTCAGTACAACATGTGCTTTTTTCTAATATTTCTATTGAGACCCATCCAACAACCATGCTTCCAAATTCAGTGATCTCACCTTGGATCCTCAAAAAAATTTCTTTGAACATTACCCCTCATCCATTTGATGCCAATTGTAACTTGTGAGATTATAACAGTTCCTTGTTGATGGCACAAGTAGAGCTTAAAGGAAAATTAGGCTTTGGAATGACTTTTCTATGAAACTTTTGATCTCCAGTCATTGAGGACTATGTGGTGTTTCTGTCCACATAGTTTTGTCACTTTTTTGTTGACATGGTAGTATATTTTGTCATATGGTTGTGACGTATATTGACCTTATTGTATAGGCCAACGCTGCATCAGGAATGGCTGTTGATGATCAGTGCAAGCTCAAATTTTTAGAGCTGAAGGCGAAAAGGACTTACCGCTTCATTGTATTTAAGATTGAAGAAAAGCAAAAGCAGGTGGTTGTGGAAAAGGTTGGTGAGCCTAACGAAAGCTACGAGGACTTCACTAAGAACATTCCTGCTGATGAATGCAGATATGCTGTGTATGATTTTGATTATGTGACTGCTGAGAATTGCCAAAAGAGCAGGATCTTTTTCATTGCATGGTAATTTCTTGATCAGCCAAAACAAAAGCACTCAGTAAAGTGATTGATTATATTAACCCGTCCAATTGATGGTAGTCTCTGTGATTGATTAGATTATATTAACCCTTTGAATTGCTTTGAAAGGTCCCCCGACAATGCAAAGGTGAGAAGCAAGATGATATACGCAAGCTCAAAGGACAGGTTCAAGAGAGAGCTGGATGGTATTCAAGTTGAATTGCAAGCAACCGATCCATCTGAGATGGATTTGGATGTTATTAAAAGCCGTGTCGTGTAATGCTTCACACGATGCTTCACTGCCTTGATTGTGCGTTATCTATACGGTCCACTTTACCTATGTTCAAATGTTAATACCTTCCTAAGGGGCAGTAGAATTTGCTTACATATAATATTTGATGGTTGTTGCAGTGTTATACTGATGTCTTTTTTCTTCTGGTTCGGTAAATTCGGAGAATTACATTAAAGATGTGTTATTTGTGGGATTTCACTGGTGAGCCTAGCTTCAATGTGTATGCTTGGCTATCTGTTTTGTGTATTCATTTTCTGGGGTGAGAATATCCTCTGATATGAGATAAGAGGACCTCTTTTCTGTATCACTAATCCATGAAACTTTTTGGCTGTTGCTGACATATGGAGTCCTTGATCCTTCTAATCCATGAAACCTTTTGGCTATTGCTGATATATGGTGTCCTTGGTCTTCTCTTTAAATGTATGTGTACTGTTCCTGCTCTAACTCAGGTTTTTGTTTCTTTATTTCTCTTGTTCTTGTTACAACTTCCTGTTCTTGCTTCTTCAAGTTGTAACAGTTTGTTGTGATCATAAAAGATTAATTAACCTTTTCCAACTACTTCAGTTGATGTTTCTTGGTTTGAGTTTGGTAACAGAAAAGTACTTAAAATCTTGTGATTGTTTTTTTGGGCACAAATATCCTGACCTCTCTCAAGGTTCACCACCCACATGCCCAAACCTCTTTCGGTGATAATGAACTCGAACCACCCAGGTGGAAGAGCTTACCACCCTTTC

mRNA sequence

ATACCCCACAGAAAGAAAAGAAAAGAAAAATACACACATAGAGAAATTTAAATTAAAAAAAGAAAAGAAAAAAAAGAAATCAGTCTCCATTTTATACCTCCCTTATTTCCCATTCTCTCTCTATCTGCGTCTTTCTCTCTCCATCAAAGCCCTATATTCTCTCCATTTTCTCTCTCTCCTACTCTCACAGGTGCTGCTACCACTTCACTCAGAACAAGAACAAAAACAAAAATGGCCAACGCTGCATCAGGAATGGCTGTTGATGATCAGTGCAAGCTCAAATTTTTAGAGCTGAAGGCGAAAAGGACTTACCGCTTCATTGTATTTAAGATTGAAGAAAAGCAAAAGCAGGTGGTTGTGGAAAAGGTTGGTGAGCCTAACGAAAGCTACGAGGACTTCACTAAGAACATTCCTGCTGATGAATGCAGATATGCTGTGTATGATTTTGATTATGTGACTGCTGAGAATTGCCAAAAGAGCAGGATCTTTTTCATTGCATGGTCCCCCGACAATGCAAAGGTGAGAAGCAAGATGATATACGCAAGCTCAAAGGACAGGTTCAAGAGAGAGCTGGATGGTATTCAAGTTGAATTGCAAGCAACCGATCCATCTGAGATGGATTTGGATGTTATTAAAAGCCGTGTCGTGTAATGCTTCACACGATGCTTCACTGCCTTGATTGTGCGTTATCTATACGGTCCACTTTACCTATGTTCAAATGTTAATACCTTCCTAAGGGGCAGTAGAATTTGCTTACATATAATATTTGATGGTTGTTGCAGTGTTATACTGATGTCTTTTTTCTTCTGGTTCGGTAAATTCGGAGAATTACATTAAAGATGTGTTATTTGTGGGATTTCACTGGTGAGCCTAGCTTCAATGTGTATGCTTGGCTATCTGTTTTGTGTATTCATTTTCTGGGGTGAGAATATCCTCTGATATGAGATAAGAGGACCTCTTTTCTGTATCACTAATCCATGAAACTTTTTGGCTGTTGCTGACATATGGAGTCCTTGATCCTTCTAATCCATGAAACCTTTTGGCTATTGCTGATATATGGTGTCCTTGGTCTTCTCTTTAAATGTATGTGTACTGTTCCTGCTCTAACTCAGGTTTTTGTTTCTTTATTTCTCTTGTTCTTGTTACAACTTCCTGTTCTTGCTTCTTCAAGTTGTAACAGTTTGTTGTGATCATAAAAGATTAATTAACCTTTTCCAACTACTTCAGTTGATGTTTCTTGGTTTGAGTTTGGTAACAGAAAAGTACTTAAAATCTTGTGATTGTTTTTTTGGGCACAAATATCCTGACCTCTCTCAAGGTTCACCACCCACATGCCCAAACCTCTTTCGGTGATAATGAACTCGAACCACCCAGGTGGAAGAGCTTACCACCCTTTC

Coding sequence (CDS)

ATGGCCAACGCTGCATCAGGAATGGCTGTTGATGATCAGTGCAAGCTCAAATTTTTAGAGCTGAAGGCGAAAAGGACTTACCGCTTCATTGTATTTAAGATTGAAGAAAAGCAAAAGCAGGTGGTTGTGGAAAAGGTTGGTGAGCCTAACGAAAGCTACGAGGACTTCACTAAGAACATTCCTGCTGATGAATGCAGATATGCTGTGTATGATTTTGATTATGTGACTGCTGAGAATTGCCAAAAGAGCAGGATCTTTTTCATTGCATGGTCCCCCGACAATGCAAAGGTGAGAAGCAAGATGATATACGCAAGCTCAAAGGACAGGTTCAAGAGAGAGCTGGATGGTATTCAAGTTGAATTGCAAGCAACCGATCCATCTGAGATGGATTTGGATGTTATTAAAAGCCGTGTCGTGTAA

Protein sequence

MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNIPADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVELQATDPSEMDLDVIKSRVV

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo25288	Spo25288	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo25288.1	Spo25288.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25288.1.exon.1	Spo25288.1.exon.1	exon
Spo25288.1.exon.2	Spo25288.1.exon.2	exon
Spo25288.1.exon.3	Spo25288.1.exon.3	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25288.1.utr5p.1	Spo25288.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25288.1.CDS.1	Spo25288.1.CDS.1	CDS
Spo25288.1.CDS.2	Spo25288.1.CDS.2	CDS
Spo25288.1.CDS.3	Spo25288.1.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25288.1.utr3p.1	Spo25288.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo25288.1 vs. NCBI nr
Match: gi|902197140|gb|KNA13337.1| (hypothetical protein SOVF_117910 [Spinacia oleracea])

HSP 1 Score: 275.0 bits (702), Expect = 7.100e-71
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRVV 140
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25288.1 vs. NCBI nr
Match: gi|902111342|gb|KNA03751.1| (hypothetical protein SOVF_206070 [Spinacia oleracea])

HSP 1 Score: 274.6 bits (701), Expect = 9.300e-71
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQV+VEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRVV 140
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25288.1 vs. NCBI nr
Match: gi|731318670|ref|XP_010669850.1| (PREDICTED: actin-depolymerizing factor 4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 258.1 bits (658), Expect = 9.000e-66
Identity = 128/137 (93.43%), Postives = 133/137 (97.08%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQV+VEKVGEP+ESYEDF  +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFAASI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD+VTAENCQKSRIFFIAWSPD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWSPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25288.1 vs. NCBI nr
Match: gi|731318668|ref|XP_010669849.1| (PREDICTED: actin-depolymerizing factor 4-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 257.7 bits (657), Expect = 1.200e-65
Identity = 127/137 (92.70%), Postives = 134/137 (97.81%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQV+VEKVGEP+ESYEDFT +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFTASI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD+VTAENCQKSRIFFIAW+PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWAPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDP+EMDLDVIKSR
Sbjct: 121 LQATDPTEMDLDVIKSR 137

[Close]

BLAST of Spo25288.1 vs. NCBI nr
Match: gi|763811997|gb|KJB78849.1| (hypothetical protein B456_013G022900 [Gossypium raimondii])

HSP 1 Score: 248.8 bits (634), Expect = 5.500e-63
Identity = 125/137 (91.24%), Postives = 130/137 (94.89%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEP +SYE FT ++
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTDSYEAFTASL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD+VT ENCQKSRIFFIAWSPD +KVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTDENCQKSRIFFIAWSPDTSKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R3F8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_117910 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 5.000e-71
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRVV 140
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QB58_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_206070 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 6.500e-71
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQV+VEKVGEPNESYEDFTKNI
Sbjct: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVIVEKVGEPNESYEDFTKNI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRVV 140
           LQATDPSEMDLDVIKSRVV
Sbjct: 121 LQATDPSEMDLDVIKSRVV 139

[Close]

BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D098_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g037400 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 6.300e-66
Identity = 128/137 (93.43%), Postives = 133/137 (97.08%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQV+VEKVGEP+ESYEDF  +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFAASI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD+VTAENCQKSRIFFIAWSPD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWSPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CVB6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g037390 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 8.200e-66
Identity = 127/137 (92.70%), Postives = 134/137 (97.81%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAVDDQCKLKFLELK KRTYRFI+FKIEEKQKQV+VEKVGEP+ESYEDFT +I
Sbjct: 1   MANAASGMAVDDQCKLKFLELKTKRTYRFIIFKIEEKQKQVIVEKVGEPSESYEDFTASI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD+VTAENCQKSRIFFIAW+PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTAENCQKSRIFFIAWAPDTAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDP+EMDLDVIKSR
Sbjct: 121 LQATDPTEMDLDVIKSR 137

[Close]

BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
Match: A0A0D2V9F2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G022900 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 3.800e-63
Identity = 125/137 (91.24%), Postives = 130/137 (94.89%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEP +SYE FT ++
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTDSYEAFTASL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD+VT ENCQKSRIFFIAWSPD +KVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFVTDENCQKSRIFFIAWSPDTSKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDPSEMDLDVIKSR
Sbjct: 121 LQATDPSEMDLDVIKSR 137

[Close]

BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
Match: ADF4_ARATH (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana GN=ADF4 PE=1 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 2.100e-62
Identity = 118/138 (85.51%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDF  ++
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRV 139
           LQATDP+EMDLDV+KSRV
Sbjct: 121 LQATDPTEMDLDVLKSRV 138

[Close]

BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
Match: ADF2_PETHY (Actin-depolymerizing factor 2 OS=Petunia hybrida GN=ADF2 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.300e-61
Identity = 117/137 (85.40%), Postives = 127/137 (92.70%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKLKFLELKAKRTYRFI++KIEEKQK+VVVEK+GEP ESYEDFT  +
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYAVYDFD++T EN QKSRIFFIAWSPD A+VRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDP+EM LDV +SR
Sbjct: 121 LQATDPTEMGLDVFRSR 137

[Close]

BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
Match: ADF1_PETHY (Actin-depolymerizing factor 1 OS=Petunia hybrida GN=ADF1 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 3.900e-61
Identity = 116/137 (84.67%), Postives = 127/137 (92.70%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQVVVEK+GEP ESYEDF  ++
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           P +ECRYAVYDFD+VTAENCQKSRIFFIAW PD A+VRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQA DP+EM LDVI+SR
Sbjct: 121 LQACDPTEMGLDVIQSR 137

[Close]

BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
Match: ADF1_ARATH (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana GN=ADF1 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 8.700e-61
Identity = 115/137 (83.94%), Postives = 128/137 (93.43%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P ++YE+F   +
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDP+EMDLDV +SR
Sbjct: 121 LQATDPTEMDLDVFRSR 137

[Close]

BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
Match: ADF10_ARATH (Actin-depolymerizing factor 10 OS=Arabidopsis thaliana GN=ADF10 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 3.700e-59
Identity = 108/138 (78.26%), Postives = 126/138 (91.30%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MAN+ASGM V D+CKLKFLELKAKR YRFIVFKI+EK +QV+++K+G P E+YEDFT++I
Sbjct: 1   MANSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           P DECRYAVYD+D+ T ENCQKS+IFFIAWSPD ++VRSKM+YASSKDRFKRELDGIQVE
Sbjct: 61  PEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRV 139
           LQATDPSEM LD+IK RV
Sbjct: 121 LQATDPSEMSLDIIKGRV 138

[Close]

BLAST of Spo25288.1 vs. TAIR (Arabidopsis)
Match: AT5G59890.1 (actin depolymerizing factor 4)

HSP 1 Score: 239.6 bits (610), Expect = 1.200e-63
Identity = 118/138 (85.51%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDF  ++
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRV 139
           LQATDP+EMDLDV+KSRV
Sbjct: 121 LQATDPTEMDLDVLKSRV 138

[Close]

BLAST of Spo25288.1 vs. TAIR (Arabidopsis)
Match: AT3G46010.2 (actin depolymerizing factor 1)

HSP 1 Score: 232.3 bits (591), Expect = 1.900e-61
Identity = 114/136 (83.82%), Postives = 127/136 (93.38%), Query Frame = 1


		  
Query: 2   ANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNIP 61
           ANAASGMAV D CKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P ++YE+F   +P
Sbjct: 13  ANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP 72

Query: 62  ADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVEL 121
           ADECRYA+YDFD+VTAENCQKS+IFFIAW PD AKVRSKMIYASSKDRFKRELDGIQVEL
Sbjct: 73  ADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVEL 132

Query: 122 QATDPSEMDLDVIKSR 138
           QATDP+EMDLDV +SR
Sbjct: 133 QATDPTEMDLDVFRSR 148

[Close]

BLAST of Spo25288.1 vs. TAIR (Arabidopsis)
Match: AT1G01750.1 (actin depolymerizing factor 11)

HSP 1 Score: 228.8 bits (582), Expect = 2.100e-60
Identity = 108/138 (78.26%), Postives = 126/138 (91.30%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MAN+ASGM V D+CKLKFLELKAKR YRFIVFKI+EK +QV+++K+G P E+YEDFT++I
Sbjct: 1   MANSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           P DECRYAVYD+D+ T ENCQKS+IFFIAWSPD ++VRSKM+YASSKDRFKRELDGIQVE
Sbjct: 61  PEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRV 139
           LQATDPSEM LD+IK RV
Sbjct: 121 LQATDPSEMSLDIIKGRV 138

[Close]

BLAST of Spo25288.1 vs. TAIR (Arabidopsis)
Match: AT4G00680.1 (actin depolymerizing factor 8)

HSP 1 Score: 224.6 bits (571), Expect = 3.900e-59
Identity = 105/138 (76.09%), Postives = 126/138 (91.30%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MAN+ASGM V+D+CK+KFLELKAKRTYRFIVFKI+EK +QV +EK+G P E+Y+DFT +I
Sbjct: 1   MANSASGMHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSI 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           P DECRYAVYDFD+ T +NCQKS+IFFIAWSPD ++VRSKM+YASSKDRFKRE++GIQVE
Sbjct: 61  PDDECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVE 120

Query: 121 LQATDPSEMDLDVIKSRV 139
           LQATDPSEM LD+IK R+
Sbjct: 121 LQATDPSEMSLDIIKGRL 138

[Close]

BLAST of Spo25288.1 vs. TAIR (Arabidopsis)
Match: AT3G46000.1 (actin depolymerizing factor 2)

HSP 1 Score: 223.4 bits (568), Expect = 8.700e-59
Identity = 109/137 (79.56%), Postives = 124/137 (90.51%), Query Frame = 1


		  
Query: 1   MANAASGMAVDDQCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPNESYEDFTKNI 60
           MANAASGMAV D CKLKF+ELKAKRT+R IV+KIE+  KQV+VEK+GEP +SY+DF  ++
Sbjct: 1   MANAASGMAVHDDCKLKFMELKAKRTFRTIVYKIED--KQVIVEKLGEPEQSYDDFAASL 60

Query: 61  PADECRYAVYDFDYVTAENCQKSRIFFIAWSPDNAKVRSKMIYASSKDRFKRELDGIQVE 120
           PAD+CRY +YDFD+VTAENCQKS+IFFIAWSPD AKVR KMIYASSKDRFKRELDGIQVE
Sbjct: 61  PADDCRYCIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVIKSR 138
           LQATDP+EM LDV KSR
Sbjct: 121 LQATDPTEMGLDVFKSR 135

The following BLAST results are available for this feature:

BLAST of Spo25288.1 vs. NCBI nr
BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
BLAST of Spo25288.1 vs. TAIR (Arabidopsis)

BLAST of Spo25288.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902197140\|gb\|KNA13337.1\|	7.1e-71	100.	hypothetical protein SOVF_1179...	[more]
gi\|902111342\|gb\|KNA03751.1\|	9.3e-71	99.2	hypothetical protein SOVF_2060...	[more]
gi\|731318670\|ref\|XP_010669850.1\|	9.0e-66	93.4	PREDICTED: actin-depolymerizin...	[more]
gi\|731318668\|ref\|XP_010669849.1\|	1.2e-65	92.7	PREDICTED: actin-depolymerizin...	[more]
gi\|763811997\|gb\|KJB78849.1\|	5.5e-63	91.2	hypothetical protein B456_013G...	[more]

BLAST of Spo25288.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R3F8_SPIOL	5.0e-71	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QB58_SPIOL	6.5e-71	99.2	Uncharacterized protein OS=Spi...	[more]
A0A0J8D098_BETVU	6.3e-66	93.4	Uncharacterized protein OS=Bet...	[more]
A0A0J8CVB6_BETVU	8.2e-66	92.7	Uncharacterized protein OS=Bet...	[more]
A0A0D2V9F2_GOSRA	3.8e-63	91.2	Uncharacterized protein OS=Gos...	[more]

BLAST of Spo25288.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ADF4_ARATH	2.1e-62	85.5	Actin-depolymerizing factor 4 ...	[more]
ADF2_PETHY	1.3e-61	85.4	Actin-depolymerizing factor 2 ...	[more]
ADF1_PETHY	3.9e-61	84.6	Actin-depolymerizing factor 1 ...	[more]
ADF1_ARATH	8.7e-61	83.9	Actin-depolymerizing factor 1 ...	[more]
ADF10_ARATH	3.7e-59	78.2	Actin-depolymerizing factor 10...	[more]

BLAST of Spo25288.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G59890.1	1.2e-63	85.5	actin depolymerizing factor 4	[more]
AT3G46010.2	1.9e-61	83.8	actin depolymerizing factor 1	[more]
AT1G01750.1	2.1e-60	78.2	actin depolymerizing factor 11	[more]
AT4G00680.1	3.9e-59	76.0	actin depolymerizing factor 8	[more]
AT3G46000.1	8.7e-59	79.5	actin depolymerizing factor 2	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 14..136 score: 4.9
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 12..139 score: 4.5
IPR002108	Actin-depolymerising factor homology domain	PROFILE	PS51263	ADF_H	coord: 7..139 score: 40
IPR017904	ADF/Cofilin/Destrin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 1..138 score: 1.4E
None	No IPR available	PANTHER	PTHR11913:SF30	ACTIN-DEPOLYMERIZING FACTOR 10-RELATED	coord: 1..138 score: 1.4E
None	No IPR available	unknown	SSF55753	Actin depolymerizing proteins	coord: 3..138 score: 2.97

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003779	actin binding