Spo23965 (gene)

BLAST of Spo23965.1 vs. NCBI nr
Match: gi|902185582|gb|KNA10518.1| (hypothetical protein SOVF_143700 [Spinacia oleracea])

HSP 1 Score: 913.7 bits (2360), Expect = 1.300e-262
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 1

		  

Query: 7   MPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVH 66
           MPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVH
Sbjct: 1   MPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVH 60

Query: 67  KSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDL 126
           KSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDL
Sbjct: 61  KSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDL 120

Query: 127 SAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAG 186
           SAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAG
Sbjct: 121 SAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAG 180

Query: 187 TIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLV 246
           TIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLV
Sbjct: 181 TIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLV 240

Query: 247 IVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSW 306
           IVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSW
Sbjct: 241 IVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSW 300

Query: 307 GVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPR 366
           GVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPR
Sbjct: 301 GVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPR 360

Query: 367 LSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVD 426
           LSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVD
Sbjct: 361 LSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVD 420

Query: 427 YPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS 466
           YPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS
Sbjct: 421 YPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS 459

		  

BLAST of Spo23965.1 vs. NCBI nr
Match: gi|731328706|ref|XP_010675198.1| (PREDICTED: diphthamide biosynthesis protein 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 790.4 bits (2040), Expect = 1.700e-225
Identity = 399/470 (84.89%), Postives = 429/470 (91.28%), Query Frame = 1

		  

Query: 1   MEKNTEMPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPN 60
           MEKN E P+P    PP +TITTT  TT K+QPKRFIRNQIP+SILNNPSLNAAISLLPPN
Sbjct: 1   MEKNPETPSP----PPSSTITTTT-TTPKAQPKRFIRNQIPASILNNPSLNAAISLLPPN 60

Query: 61  YNFEVHKSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGA 120
           YNFE+HKS+HRI SLSSPSPR+ALQLPEGLLM+SLSLSDIFRTFAS SDVF+LADVTYGA
Sbjct: 61  YNFEIHKSIHRINSLSSPSPRIALQLPEGLLMFSLSLSDIFRTFASVSDVFILADVTYGA 120

Query: 121 CCVDDLSAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSR 180
           CCVDDLSAAALGADLLIHYGHSCLVPVD+TVIPCLYVFVEIG+DV KLV+T++ NDLGSR
Sbjct: 121 CCVDDLSAAALGADLLIHYGHSCLVPVDSTVIPCLYVFVEIGVDVKKLVETVKINDLGSR 180

Query: 181 SLAVAGTIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDD 240
           SLA+AGTIQF GAIRGAK+ELE+LGFRVLVPQ+KPLS GEVLGCTAPRVSK+DFG    +
Sbjct: 181 SLAIAGTIQFGGAIRGAKVELEKLGFRVLVPQSKPLSGGEVLGCTAPRVSKRDFGDGGGE 240

Query: 241 EGEDLVIVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKA 300
           E EDLVI+FVADGRFHLEAFMIANP VKA+RYDPYLGRLFLEEYDHKGMKSSRMKAVLKA
Sbjct: 241 E-EDLVILFVADGRFHLEAFMIANPGVKAYRYDPYLGRLFLEEYDHKGMKSSRMKAVLKA 300

Query: 301 REAKSWGVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWI 360
           +EAKSWG+VLGTLGRQGNPK LERLERKMEEKGMSWTVVLMSEISPTRI LFEDS+DAWI
Sbjct: 301 KEAKSWGIVLGTLGRQGNPKILERLERKMEEKGMSWTVVLMSEISPTRIGLFEDSVDAWI 360

Query: 361 QIACPRLSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEA 420
           QIACPRLSIDWGEAF RPLL PFEAEIALGLIRGWWERD S VG DCG N GSCGCD+  
Sbjct: 361 QIACPRLSIDWGEAFVRPLLTPFEAEIALGLIRGWWERDGSGVGGDCGGNGGSCGCDDGT 420

Query: 421 NKGGV-----DYPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS 466
           NKGGV     DYPMDYYAQDGGEWNSSY KG+NRP+RRNLR +VA+G VS
Sbjct: 421 NKGGVEQNGGDYPMDYYAQDGGEWNSSYMKGRNRPTRRNLRPSVANGTVS 464

		  

BLAST of Spo23965.1 vs. NCBI nr
Match: gi|593787988|ref|XP_007157033.1| (hypothetical protein PHAVU_002G038000g [Phaseolus vulgaris])

HSP 1 Score: 601.7 bits (1550), Expect = 1.100e-168
Identity = 303/439 (69.02%), Postives = 355/439 (80.87%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K +PKRF++NQ+P SILN+P LNAAIS+LP NYNFE+HKSV R+LS  +   RVALQ PE
Sbjct: 27  KPKPKRFVKNQVPDSILNDPLLNAAISVLPSNYNFEIHKSVWRVLSTGAK--RVALQFPE 86

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMYSLSLSDIF TFA+ +  ++L DVTYGACCVDD +AAAL ADLLIHYGHSCLVP+D
Sbjct: 87  GLLMYSLSLSDIFTTFAAVTHCYILGDVTYGACCVDDFAAAALDADLLIHYGHSCLVPID 146

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETN-DLGSRSLAVAGTIQFSGAIRGAKIELERLGFR 208
            T IPCLYVFV+I IDV   V T+  N +   ++L VAGTIQF+ AIR AK +LE LGFR
Sbjct: 147 ATTIPCLYVFVDIKIDVPHFVDTVRLNLESRPQTLVVAGTIQFASAIRAAKPQLEELGFR 206

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VLVPQ+KPLSAGEVLGCTAPRVS    G  V +     V+VFVADGRFHLEAFMIANP +
Sbjct: 207 VLVPQSKPLSAGEVLGCTAPRVSSNSLGCGVGES----VLVFVADGRFHLEAFMIANPGI 266

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           +AFRYDPY+G+LFLEEYDH GMK SR  A+LKAREA+SWGVVLGTLGRQGNP+ LERLE+
Sbjct: 267 RAFRYDPYMGKLFLEEYDHVGMKRSRKNAILKAREARSWGVVLGTLGRQGNPRILERLEK 326

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
           KM E+G+ +TVVLMSE+SP R+ALFEDS+D+WIQIACPRLSIDWGEAF +P+L  FEA++
Sbjct: 327 KMRERGLDYTVVLMSELSPGRVALFEDSVDSWIQIACPRLSIDWGEAFVKPVLTSFEADV 386

Query: 389 ALGLIRGWWERDN-SDVGVDCGRNSGSCGCDNEANKGGVDYPMDYYAQDGGEWNSSYTKG 448
           ALGLI GWWE++   D  V CG+ SGSC C+ +A   G DYPMDYYAQDGGEWNSSY K 
Sbjct: 387 ALGLIPGWWEKNEVCDESVGCGKRSGSC-CEGDAKDFGGDYPMDYYAQDGGEWNSSYMKN 446

Query: 449 KNRPSRRNLRSNVADGRVS 466
             RP+RR   S+VA   +S
Sbjct: 447 STRPARRLSVSSVAATAIS 458

		  

BLAST of Spo23965.1 vs. NCBI nr
Match: gi|965615082|dbj|BAU00715.1| (hypothetical protein VIGAN_10233100 [Vigna angularis var. angularis])

HSP 1 Score: 601.3 bits (1549), Expect = 1.500e-168
Identity = 306/442 (69.23%), Postives = 358/442 (81.00%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K +PKRF++NQIP SILN+P LNAAIS+LP NYNFE+HKSV R+LS  +   RVALQ PE
Sbjct: 33  KPKPKRFVKNQIPDSILNDPLLNAAISVLPSNYNFEIHKSVWRVLSTGAK--RVALQFPE 92

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMYSLSLSDIF TFA+ +  ++L DVTYGACCVDD SAAALGADLLIHYGHSCLVP+D
Sbjct: 93  GLLMYSLSLSDIFTTFAAVTHCYILGDVTYGACCVDDFSAAALGADLLIHYGHSCLVPID 152

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETN-DLGSRSLAVAGTIQFSGAIRGAKIELERLGFR 208
            T IPCLYVFV+I IDV   V T+  N +   ++L VAGTIQF+ AIR AK +LE LGFR
Sbjct: 153 ATTIPCLYVFVDIKIDVPHFVDTVRLNLESRPQTLVVAGTIQFASAIRAAKPQLEELGFR 212

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VLVPQ+KPLSAGEVLGCTAPRVS    G  V +     V+VFVADGRFHLEAFMIANP +
Sbjct: 213 VLVPQSKPLSAGEVLGCTAPRVSANSLGCGVGES----VLVFVADGRFHLEAFMIANPGI 272

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           +AFRYDPY+G+LFLEEYDH GMK SR  A+LKAREA+SWGVVLGTLGRQGNP+ LERLE+
Sbjct: 273 RAFRYDPYMGKLFLEEYDHVGMKRSRKNAILKAREARSWGVVLGTLGRQGNPRILERLEK 332

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
           KM+E+G+ +TVVLMSE+SP R+ALFEDS+DAWIQIACPRLSIDWGEAF +P+L  FEAE+
Sbjct: 333 KMKERGLDYTVVLMSELSPGRVALFEDSVDAWIQIACPRLSIDWGEAFVKPVLTSFEAEV 392

Query: 389 ALGLIRGWWERDN-SDVGVDCGRNSGSCGCDNEANKG---GVDYPMDYYAQDGGEWNSSY 448
           ALG+I GWWE++   + GV C + SG+C C+ +A  G   G DYPMDYYAQDGGEWNSSY
Sbjct: 393 ALGVIPGWWEKNEVCEDGVGCCKRSGAC-CEGDAKGGEDFGGDYPMDYYAQDGGEWNSSY 452

Query: 449 TKGKNRPSRRNLRSNVADGRVS 466
            K   RP+RR   S+VA   +S
Sbjct: 453 VKKSTRPARRVSVSSVAGAAIS 467

		  

BLAST of Spo23965.1 vs. NCBI nr
Match: gi|947075511|gb|KRH24351.1| (hypothetical protein GLYMA_12G035900 [Glycine max])

HSP 1 Score: 599.7 bits (1545), Expect = 4.200e-168
Identity = 308/440 (70.00%), Postives = 355/440 (80.68%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K +PKRF++NQIP SILNNP L+AAIS+LP NYNFEVHK V R+LS  +   RVALQ PE
Sbjct: 23  KPKPKRFVKNQIPDSILNNPLLSAAISVLPSNYNFEVHKCVWRVLSTGAK--RVALQFPE 82

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMYSL LSDIF TFA  +  FVLADVTYGACCVDDL+A+ALGADLLIHYGHSCLVP+D
Sbjct: 83  GLLMYSLPLSDIFTTFAGVTHCFVLADVTYGACCVDDLAASALGADLLIHYGHSCLVPID 142

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETNDLG-SRSLAVAGTIQFSGAIRGAKIELERLGFR 208
            T IPCLYVFV+I IDV   V T+  N L  +++L VAGTIQF+ AIR AK +LE LGFR
Sbjct: 143 ATTIPCLYVFVDIKIDVPHFVDTLSLNLLAQTKTLVVAGTIQFASAIRAAKPQLEELGFR 202

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VL+PQ+KPLSAGEVLGCTAP+VS     G   D GE  V+VFVADGRFHLEAFMIANP +
Sbjct: 203 VLIPQSKPLSAGEVLGCTAPKVSSSKLLGGGGDIGE-CVLVFVADGRFHLEAFMIANPGI 262

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           +AFRYDPY+G+LFLEEYDH GMK SR  A+ KAREA+SWG+VLGTLGRQGNP+ LERLER
Sbjct: 263 RAFRYDPYMGKLFLEEYDHLGMKRSRKNAIFKAREARSWGLVLGTLGRQGNPRILERLER 322

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
            M E+G+ +TVVLMSE+SPTRIALFEDS+DAWIQIACPRLSIDWGEAF +P+L PFEAE+
Sbjct: 323 MMRERGLDYTVVLMSEMSPTRIALFEDSLDAWIQIACPRLSIDWGEAFVKPVLTPFEAEV 382

Query: 389 ALGLIRGWWERDN-SDVGVDCGRNSGSCGCDNEANKG-GVDYPMDYYAQDGGEWNSSYTK 448
           ALG+I GWWE++   + G  C   SGSC  D +  +  G DYPMDYYAQDGGEWNSSY K
Sbjct: 383 ALGVIPGWWEKNEVCEEGEGCCNKSGSCCEDAKGGEDFGGDYPMDYYAQDGGEWNSSYVK 442

Query: 449 GKNRPSRRNLRSNVADGRVS 466
              RP+RR   S+VAD  +S
Sbjct: 443 KSTRPARRVSVSSVADTAIS 459

		  

BLAST of Spo23965.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QUY2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_143700 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 9.200e-263
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 1

		  

Query: 7   MPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVH 66
           MPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVH
Sbjct: 1   MPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVH 60

Query: 67  KSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDL 126
           KSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDL
Sbjct: 61  KSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDL 120

Query: 127 SAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAG 186
           SAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAG
Sbjct: 121 SAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAG 180

Query: 187 TIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLV 246
           TIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLV
Sbjct: 181 TIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLV 240

Query: 247 IVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSW 306
           IVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSW
Sbjct: 241 IVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSW 300

Query: 307 GVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPR 366
           GVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPR
Sbjct: 301 GVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPR 360

Query: 367 LSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVD 426
           LSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVD
Sbjct: 361 LSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVD 420

Query: 427 YPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS 466
           YPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS
Sbjct: 421 YPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS 459

		  

BLAST of Spo23965.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CMV5_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g084450 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.200e-225
Identity = 399/470 (84.89%), Postives = 429/470 (91.28%), Query Frame = 1

		  

Query: 1   MEKNTEMPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPN 60
           MEKN E P+P    PP +TITTT  TT K+QPKRFIRNQIP+SILNNPSLNAAISLLPPN
Sbjct: 1   MEKNPETPSP----PPSSTITTTT-TTPKAQPKRFIRNQIPASILNNPSLNAAISLLPPN 60

Query: 61  YNFEVHKSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGA 120
           YNFE+HKS+HRI SLSSPSPR+ALQLPEGLLM+SLSLSDIFRTFAS SDVF+LADVTYGA
Sbjct: 61  YNFEIHKSIHRINSLSSPSPRIALQLPEGLLMFSLSLSDIFRTFASVSDVFILADVTYGA 120

Query: 121 CCVDDLSAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSR 180
           CCVDDLSAAALGADLLIHYGHSCLVPVD+TVIPCLYVFVEIG+DV KLV+T++ NDLGSR
Sbjct: 121 CCVDDLSAAALGADLLIHYGHSCLVPVDSTVIPCLYVFVEIGVDVKKLVETVKINDLGSR 180

Query: 181 SLAVAGTIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDD 240
           SLA+AGTIQF GAIRGAK+ELE+LGFRVLVPQ+KPLS GEVLGCTAPRVSK+DFG    +
Sbjct: 181 SLAIAGTIQFGGAIRGAKVELEKLGFRVLVPQSKPLSGGEVLGCTAPRVSKRDFGDGGGE 240

Query: 241 EGEDLVIVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKA 300
           E EDLVI+FVADGRFHLEAFMIANP VKA+RYDPYLGRLFLEEYDHKGMKSSRMKAVLKA
Sbjct: 241 E-EDLVILFVADGRFHLEAFMIANPGVKAYRYDPYLGRLFLEEYDHKGMKSSRMKAVLKA 300

Query: 301 REAKSWGVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWI 360
           +EAKSWG+VLGTLGRQGNPK LERLERKMEEKGMSWTVVLMSEISPTRI LFEDS+DAWI
Sbjct: 301 KEAKSWGIVLGTLGRQGNPKILERLERKMEEKGMSWTVVLMSEISPTRIGLFEDSVDAWI 360

Query: 361 QIACPRLSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEA 420
           QIACPRLSIDWGEAF RPLL PFEAEIALGLIRGWWERD S VG DCG N GSCGCD+  
Sbjct: 361 QIACPRLSIDWGEAFVRPLLTPFEAEIALGLIRGWWERDGSGVGGDCGGNGGSCGCDDGT 420

Query: 421 NKGGV-----DYPMDYYAQDGGEWNSSYTKGKNRPSRRNLRSNVADGRVS 466
           NKGGV     DYPMDYYAQDGGEWNSSY KG+NRP+RRNLR +VA+G VS
Sbjct: 421 NKGGVEQNGGDYPMDYYAQDGGEWNSSYMKGRNRPTRRNLRPSVANGTVS 464

		  

BLAST of Spo23965.1 vs. UniProtKB/TrEMBL
Match: V7CFP8_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G038000g PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 7.700e-169
Identity = 303/439 (69.02%), Postives = 355/439 (80.87%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K +PKRF++NQ+P SILN+P LNAAIS+LP NYNFE+HKSV R+LS  +   RVALQ PE
Sbjct: 27  KPKPKRFVKNQVPDSILNDPLLNAAISVLPSNYNFEIHKSVWRVLSTGAK--RVALQFPE 86

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMYSLSLSDIF TFA+ +  ++L DVTYGACCVDD +AAAL ADLLIHYGHSCLVP+D
Sbjct: 87  GLLMYSLSLSDIFTTFAAVTHCYILGDVTYGACCVDDFAAAALDADLLIHYGHSCLVPID 146

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETN-DLGSRSLAVAGTIQFSGAIRGAKIELERLGFR 208
            T IPCLYVFV+I IDV   V T+  N +   ++L VAGTIQF+ AIR AK +LE LGFR
Sbjct: 147 ATTIPCLYVFVDIKIDVPHFVDTVRLNLESRPQTLVVAGTIQFASAIRAAKPQLEELGFR 206

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VLVPQ+KPLSAGEVLGCTAPRVS    G  V +     V+VFVADGRFHLEAFMIANP +
Sbjct: 207 VLVPQSKPLSAGEVLGCTAPRVSSNSLGCGVGES----VLVFVADGRFHLEAFMIANPGI 266

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           +AFRYDPY+G+LFLEEYDH GMK SR  A+LKAREA+SWGVVLGTLGRQGNP+ LERLE+
Sbjct: 267 RAFRYDPYMGKLFLEEYDHVGMKRSRKNAILKAREARSWGVVLGTLGRQGNPRILERLEK 326

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
           KM E+G+ +TVVLMSE+SP R+ALFEDS+D+WIQIACPRLSIDWGEAF +P+L  FEA++
Sbjct: 327 KMRERGLDYTVVLMSELSPGRVALFEDSVDSWIQIACPRLSIDWGEAFVKPVLTSFEADV 386

Query: 389 ALGLIRGWWERDN-SDVGVDCGRNSGSCGCDNEANKGGVDYPMDYYAQDGGEWNSSYTKG 448
           ALGLI GWWE++   D  V CG+ SGSC C+ +A   G DYPMDYYAQDGGEWNSSY K 
Sbjct: 387 ALGLIPGWWEKNEVCDESVGCGKRSGSC-CEGDAKDFGGDYPMDYYAQDGGEWNSSYMKN 446

Query: 449 KNRPSRRNLRSNVADGRVS 466
             RP+RR   S+VA   +S
Sbjct: 447 STRPARRLSVSSVAATAIS 458

		  

BLAST of Spo23965.1 vs. UniProtKB/TrEMBL
Match: A0A0S3T635_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.10G233100 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.000e-168
Identity = 306/442 (69.23%), Postives = 358/442 (81.00%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K +PKRF++NQIP SILN+P LNAAIS+LP NYNFE+HKSV R+LS  +   RVALQ PE
Sbjct: 33  KPKPKRFVKNQIPDSILNDPLLNAAISVLPSNYNFEIHKSVWRVLSTGAK--RVALQFPE 92

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMYSLSLSDIF TFA+ +  ++L DVTYGACCVDD SAAALGADLLIHYGHSCLVP+D
Sbjct: 93  GLLMYSLSLSDIFTTFAAVTHCYILGDVTYGACCVDDFSAAALGADLLIHYGHSCLVPID 152

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETN-DLGSRSLAVAGTIQFSGAIRGAKIELERLGFR 208
            T IPCLYVFV+I IDV   V T+  N +   ++L VAGTIQF+ AIR AK +LE LGFR
Sbjct: 153 ATTIPCLYVFVDIKIDVPHFVDTVRLNLESRPQTLVVAGTIQFASAIRAAKPQLEELGFR 212

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VLVPQ+KPLSAGEVLGCTAPRVS    G  V +     V+VFVADGRFHLEAFMIANP +
Sbjct: 213 VLVPQSKPLSAGEVLGCTAPRVSANSLGCGVGES----VLVFVADGRFHLEAFMIANPGI 272

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           +AFRYDPY+G+LFLEEYDH GMK SR  A+LKAREA+SWGVVLGTLGRQGNP+ LERLE+
Sbjct: 273 RAFRYDPYMGKLFLEEYDHVGMKRSRKNAILKAREARSWGVVLGTLGRQGNPRILERLEK 332

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
           KM+E+G+ +TVVLMSE+SP R+ALFEDS+DAWIQIACPRLSIDWGEAF +P+L  FEAE+
Sbjct: 333 KMKERGLDYTVVLMSELSPGRVALFEDSVDAWIQIACPRLSIDWGEAFVKPVLTSFEAEV 392

Query: 389 ALGLIRGWWERDN-SDVGVDCGRNSGSCGCDNEANKG---GVDYPMDYYAQDGGEWNSSY 448
           ALG+I GWWE++   + GV C + SG+C C+ +A  G   G DYPMDYYAQDGGEWNSSY
Sbjct: 393 ALGVIPGWWEKNEVCEDGVGCCKRSGAC-CEGDAKGGEDFGGDYPMDYYAQDGGEWNSSY 452

Query: 449 TKGKNRPSRRNLRSNVADGRVS 466
            K   RP+RR   S+VA   +S
Sbjct: 453 VKKSTRPARRVSVSSVAGAAIS 467

		  

BLAST of Spo23965.1 vs. UniProtKB/TrEMBL
Match: I1LPW0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G035900 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.900e-168
Identity = 308/440 (70.00%), Postives = 355/440 (80.68%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K +PKRF++NQIP SILNNP L+AAIS+LP NYNFEVHK V R+LS  +   RVALQ PE
Sbjct: 23  KPKPKRFVKNQIPDSILNNPLLSAAISVLPSNYNFEVHKCVWRVLSTGAK--RVALQFPE 82

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMYSL LSDIF TFA  +  FVLADVTYGACCVDDL+A+ALGADLLIHYGHSCLVP+D
Sbjct: 83  GLLMYSLPLSDIFTTFAGVTHCFVLADVTYGACCVDDLAASALGADLLIHYGHSCLVPID 142

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETNDLG-SRSLAVAGTIQFSGAIRGAKIELERLGFR 208
            T IPCLYVFV+I IDV   V T+  N L  +++L VAGTIQF+ AIR AK +LE LGFR
Sbjct: 143 ATTIPCLYVFVDIKIDVPHFVDTLSLNLLAQTKTLVVAGTIQFASAIRAAKPQLEELGFR 202

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VL+PQ+KPLSAGEVLGCTAP+VS     G   D GE  V+VFVADGRFHLEAFMIANP +
Sbjct: 203 VLIPQSKPLSAGEVLGCTAPKVSSSKLLGGGGDIGE-CVLVFVADGRFHLEAFMIANPGI 262

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           +AFRYDPY+G+LFLEEYDH GMK SR  A+ KAREA+SWG+VLGTLGRQGNP+ LERLER
Sbjct: 263 RAFRYDPYMGKLFLEEYDHLGMKRSRKNAIFKAREARSWGLVLGTLGRQGNPRILERLER 322

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
            M E+G+ +TVVLMSE+SPTRIALFEDS+DAWIQIACPRLSIDWGEAF +P+L PFEAE+
Sbjct: 323 MMRERGLDYTVVLMSEMSPTRIALFEDSLDAWIQIACPRLSIDWGEAFVKPVLTPFEAEV 382

Query: 389 ALGLIRGWWERDN-SDVGVDCGRNSGSCGCDNEANKG-GVDYPMDYYAQDGGEWNSSYTK 448
           ALG+I GWWE++   + G  C   SGSC  D +  +  G DYPMDYYAQDGGEWNSSY K
Sbjct: 383 ALGVIPGWWEKNEVCEEGEGCCNKSGSCCEDAKGGEDFGGDYPMDYYAQDGGEWNSSYVK 442

Query: 449 GKNRPSRRNLRSNVADGRVS 466
              RP+RR   S+VAD  +S
Sbjct: 443 KSTRPARRVSVSSVADTAIS 459

		  

BLAST of Spo23965.1 vs. ExPASy Swiss-Prot
Match: DPH1_GIBZE (Diphthamide biosynthesis protein 1 OS=Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) GN=DPH1 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 6.400e-108
Identity = 203/409 (49.63%), Postives = 270/409 (66.01%), Query Frame = 1

		  

Query: 30  SQPKRFIR--NQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLP 89
           ++P+R  R  NQ+P  IL NP L AAISLLP NYNFE+ K++HRI +  S S RVALQ+P
Sbjct: 82  AKPRRAPRLLNQVPKEILENPDLKAAISLLPANYNFEIPKTIHRIQT--SGSKRVALQMP 141

Query: 90  EGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPV 149
           EGLL+++ ++SDI   F    +  ++ DVTYGACC+DD +A ALG DLL+HY HSCL+PV
Sbjct: 142 EGLLLFATTISDILTQFCPGIETLIMGDVTYGACCIDDYTARALGCDLLVHYAHSCLIPV 201

Query: 150 DNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAGTIQFSGAIRGAKIELERLGFR 209
           D T I  LYVFV+I ID + L+ ++E N    +++AV GTIQF+  I G K  LER GFR
Sbjct: 202 DVTTIKTLYVFVDISIDATHLLASLEHNFASGKTIAVVGTIQFNATIHGVKSSLERGGFR 261

Query: 210 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 269
           VLVPQ  PLS GE+LGCT+PR        + +D+  DL I+++ DGRFHLE+ MI NP +
Sbjct: 262 VLVPQIAPLSKGEILGCTSPR--------LTEDDKIDL-ILYLGDGRFHLESIMIHNPTI 321

Query: 270 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 329
            A+RYDPY  +L  E Y H+ M+S R  A+  AR A+ WG++LG LGRQGNP +L  +E+
Sbjct: 322 PAYRYDPYSRKLTRETYGHEEMQSLRRTAIHSARSARKWGLILGALGRQGNPHTLGLIEK 381

Query: 330 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 389
           +++ +G+    +L+SEI P ++AL  D I+ W+Q+ACPRLSIDWG AF RPLL P+EA +
Sbjct: 382 ELKARGIPIVHLLLSEIFPGKLALMSD-IECWVQVACPRLSIDWGYAFSRPLLTPYEALV 441

Query: 390 ALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVDYPMDYYAQDG 437
           ALG  + W                           G   YPMDYY +DG
Sbjct: 442 ALGEKKDW---------------------------GDGVYPMDYYGKDG 451

		  

BLAST of Spo23965.1 vs. ExPASy Swiss-Prot
Match: DPH1_ASPFU (Diphthamide biosynthesis protein 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN=dph1 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 5.600e-104
Identity = 191/416 (45.91%), Postives = 269/416 (64.66%), Query Frame = 1

		  

Query: 21  TTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSP 80
           T+ Q+   +  P+    NQ+P  IL++P + AAI LLP NY+FE+ K++HRI +  S + 
Sbjct: 55  TSVQRVAPRRNPRTL--NQVPPEILDDPEIQAAIELLPKNYSFEIPKTIHRIRT--SGAK 114

Query: 81  RVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYG 140
           RVALQ PEGLL+++ ++SDI   F   ++  ++ DVTYGACC+DD +A ALG DLL+HY 
Sbjct: 115 RVALQFPEGLLIFATTISDILTQFCPGTETLIMGDVTYGACCIDDYTARALGCDLLVHYA 174

Query: 141 HSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAGTIQFSGAIRGAKIE 200
           HSCL+PVD T I  LY+FV+I ID S L+ T+E N    +++A  GTIQF+  + G K  
Sbjct: 175 HSCLIPVDVTQIKTLYIFVDISIDTSHLIATLERNFQPGKTIATVGTIQFNATLHGLKPV 234

Query: 201 LERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAF 260
           LER GF+V++PQ  PLS GE+LGCT+P++S  +            +++++ DGRFHLE+ 
Sbjct: 235 LERAGFKVVIPQIAPLSKGEILGCTSPQLSPTEID----------ILLYLGDGRFHLESA 294

Query: 261 MIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPK 320
           MI NP + A+RYDPY   L  E Y H  M + R  A+  A+ AK WG++LG+LGRQGNP 
Sbjct: 295 MIHNPTIPAYRYDPYSRTLSRETYSHDEMHALRRDAINTAKSAKKWGIILGSLGRQGNPN 354

Query: 321 SLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLL 380
           ++  +E  + E+G+ +  +L+SEI P ++A   D ++ W+QIACPRLSIDWG AF RPLL
Sbjct: 355 TMAMIENHLNERGIPFVNLLLSEIFPGKLASMSD-VECWVQIACPRLSIDWGYAFPRPLL 414

Query: 381 NPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVDYPMDYYAQDG 437
            P+EA IALG +R  W+  NS V                       YPMD+YA++G
Sbjct: 415 TPYEALIALG-VREHWDSANSGV-----------------------YPMDFYAKEG 431

		  

BLAST of Spo23965.1 vs. ExPASy Swiss-Prot
Match: DPH1_NEUCR (Diphthamide biosynthesis protein 1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=dph-1 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 4.800e-103
Identity = 190/410 (46.34%), Postives = 269/410 (65.61%), Query Frame = 1

		  

Query: 30  SQPKRFIR--NQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLP 89
           +QP+R  R  NQ+P SIL++ +LN AI+LLP NY+FE+HK++HRI +L +   RVALQ+P
Sbjct: 74  AQPRRAPRMLNQVPDSILHDAALNEAIALLPSNYSFEIHKTIHRIRTLDAK--RVALQMP 133

Query: 90  EGLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPV 149
           EGLL+++ ++SDI   F    +  ++ DVTYGACC+DD +A A+G DLL+HY HSCL+PV
Sbjct: 134 EGLLLFATTISDILTQFCPGIETLIMGDVTYGACCIDDYTARAMGCDLLVHYAHSCLIPV 193

Query: 150 DNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSLAVAGTIQFSGAIRGAKIELERLGFR 209
           D T I  LYVFV+I ID S L+ T+E N    +S+A+ GTIQF+  I G +  L++ G+ 
Sbjct: 194 DVTKIKTLYVFVDISIDTSHLLATLERNFAPGKSIAMVGTIQFNATIHGVRSTLQKAGYE 253

Query: 210 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDL-VIVFVADGRFHLEAFMIANPE 269
           V++PQ  PLS GE+LGCT+P +S+      +   G+ + +I+++ DGRFHLE+ MI NP 
Sbjct: 254 VIIPQIAPLSKGEILGCTSPNLSQ-----FLTSSGKQVDMILYLGDGRFHLESIMIHNPS 313

Query: 270 VKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLE 329
           + A+RYDPY  +L  E Y H+ M+  R  A+ +A+ AK WG++LG+LGRQGNP +L  +E
Sbjct: 314 IPAYRYDPYSRKLTHETYGHEEMQDVRRSAIRQAKTAKKWGLILGSLGRQGNPNTLGLIE 373

Query: 330 RKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAE 389
            K++  G  +    +SEI P ++A+  D ++ W+Q+ACPRLSIDWG AF RPLL P+EA 
Sbjct: 374 EKLKANGTPFVNFCLSEIFPGKLAMMSD-VECWVQVACPRLSIDWGYAFPRPLLTPYEAL 433

Query: 390 IALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANKGGVDYPMDYYAQDG 437
           IAL     W                          KG   YPMDYY ++G
Sbjct: 434 IALEEKEDW-------------------------GKGA--YPMDYYGREG 448

		  

BLAST of Spo23965.1 vs. ExPASy Swiss-Prot
Match: DPH1_EMENI (Diphthamide biosynthesis protein 1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN=dph1 PE=3 SV=2)

HSP 1 Score: 375.9 bits (964), Expect = 6.200e-103
Identity = 194/434 (44.70%), Postives = 272/434 (62.67%), Query Frame = 1

		  

Query: 3   KNTEMPAPENTAPPPTTITTTQQTTTKSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYN 62
           +  +  A  +T+      T+ Q+ T +  P+    NQ+P  I  +P + AAI LLP NY+
Sbjct: 25  RTADTQAQSSTSVQDVESTSIQKATPRRTPRTL--NQVPPEISQDPDILAAIDLLPKNYS 84

Query: 63  FEVHKSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADVTYGACC 122
           FE+ K++HRI +  S + R+ALQ PEGLL+++ ++SDI   F   ++  ++ DVTYGACC
Sbjct: 85  FEIPKTIHRIRT--SGAKRIALQFPEGLLLFATTISDILTQFCPGTETLIMGDVTYGACC 144

Query: 123 VDDLSAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETNDLGSRSL 182
           +DD +A ALG DLL+HY HSCL+PVD T I  LY+FV+I ID S L+ T+E N    +++
Sbjct: 145 IDDYTARALGCDLLVHYAHSCLIPVDVTKIKTLYIFVDISIDTSHLIATLERNFQPGKTI 204

Query: 183 AVAGTIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEG 242
           A  GTIQF+  + G K  LER GF V++PQ  PLS GE+LGCT+P +S +          
Sbjct: 205 ATVGTIQFNATLHGLKPVLERAGFNVVIPQITPLSKGEILGCTSPSLSAQQID------- 264

Query: 243 EDLVIVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKARE 302
               ++++ DGRFHLE+ MI NP + A+RYDPY   L  E YDH  M + R  A+  AR 
Sbjct: 265 ---YLLYLGDGRFHLESAMIHNPSIPAYRYDPYSRTLSRESYDHTEMHTLRRDAIAAART 324

Query: 303 AKSWGVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQI 362
           AK WG++LG+LGRQGNP ++  +E  + E+G+ +  +L+SEI P ++A   D ++ W+QI
Sbjct: 325 AKKWGIILGSLGRQGNPHTMAMIESHLNERGIPFVNLLLSEIFPGKLAAMSD-VECWVQI 384

Query: 363 ACPRLSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGCDNEANK 422
           ACPRLSIDWG AF RPLL P+EA IALG +R  WE+ +  V                   
Sbjct: 385 ACPRLSIDWGYAFPRPLLTPYEALIALG-VRDDWEKTHEGV------------------- 419

Query: 423 GGVDYPMDYYAQDG 437
               YPMD+YA+DG
Sbjct: 445 ----YPMDFYAKDG 419

		  

BLAST of Spo23965.1 vs. ExPASy Swiss-Prot
Match: DPH1_DICDI (Diphthamide biosynthesis protein 1 OS=Dictyostelium discoideum GN=dph1 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 8.400e-100
Identity = 198/449 (44.10%), Postives = 280/449 (62.36%), Query Frame = 1

		  

Query: 11  ENTAPPPTTITTTQQTT--------TKSQPKRFIRN------QIPSSILNNPSLNAAISL 70
           +N     TT TTT  TT        T+ + K F R       QIP  I+N+  LN A+ +
Sbjct: 61  DNVTTTKTTTTTTTTTTPITKDIVTTEKKVKVFGRGLAMAAQQIPDEIMNDKELNLAVKI 120

Query: 71  LPPNYNFEVHKSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRTFASASDVFVLADV 130
           LP NYNFE+ K++ RI   S+   RVALQ PEGLLMYS  +SDI   FAS   + ++ DV
Sbjct: 121 LPSNYNFEIFKTIWRIKQASAK--RVALQFPEGLLMYSCIISDIIEKFASVETI-IMGDV 180

Query: 131 TYGACCVDDLSAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVKTIETND 190
           TYGACCVDD +A +LGAD ++HYGHSCL+P+D + I  LYVFV+I  D+   ++T++ N 
Sbjct: 181 TYGACCVDDYTARSLGADFMVHYGHSCLIPIDVSEIKMLYVFVDIQFDLQHFIETLKFNF 240

Query: 191 LGSRSLAVAGTIQFSGAIRGAKIELERLGFRVLVPQAKPLSAGEVLGCTAPRVSKKDFGG 250
             ++ L +  TIQFS +++ ++  L      + +PQ KPLS GE+LGCT+P++    F  
Sbjct: 241 KQTQKLIMVSTIQFSASLQSSREPLSEYFSNIFIPQEKPLSPGEILGCTSPKIK---FTS 300

Query: 251 IVDDEGEDLVIVFVADGRFHLEAFMIANPEVKAFRYDPYLGRLFLEEYDHKGMKSSRMKA 310
              DE  + +++++ DGRFHLE+ MI+NP VK++RYDPY     LE+YD + M   R  A
Sbjct: 301 PDGDEENNEIVIYLGDGRFHLESIMISNPHVKSYRYDPYSKVFSLEKYDFQEMYKIRRDA 360

Query: 311 VLKAREAKSWGVVLGTLGRQGNPKSLERLERKMEEKGMSWTVVLMSEISPTRIALFEDSI 370
           +  A +A  +G++LGTLGRQG+PK L+ LE+ ++  G  +T VL+SEI P ++ +F D I
Sbjct: 361 IETASKATKFGIILGTLGRQGSPKILDHLEQLLKSNGKHYTTVLLSEIFPAKLDMFSD-I 420

Query: 371 DAWIQIACPRLSIDWGEAFHRPLLNPFEAEIALGLIRGWWERDNSDVGVDCGRNSGSCGC 430
           ++WIQIACPRLSIDWG AF  PLLNP+EAE+ LG I   W+                   
Sbjct: 421 ESWIQIACPRLSIDWGYAFTTPLLNPYEAEVCLGGIN--WQS------------------ 472

Query: 431 DNEANKGGVDYPMDYYAQDGGEWNSSYTK 446
                     YPMD+Y+++GG+W ++Y+K
Sbjct: 481 ---------VYPMDFYSKEGGKW-TNYSK 472

		  

BLAST of Spo23965.1 vs. TAIR (Arabidopsis)
Match: AT5G62030.1 (diphthamide synthesis DPH2 family protein)

HSP 1 Score: 576.6 bits (1485), Expect = 1.300e-164
Identity = 299/441 (67.80%), Postives = 345/441 (78.23%), Query Frame = 1

		  

Query: 29  KSQPKRFIRNQIPSSILNNPSLNAAISLLPPNYNFEVHKSVHRILSLSSPSPRVALQLPE 88
           K  PKRFI+NQIP SIL + SLNAAIS+LP  Y+FEVHK V RI +  S + R+ALQLPE
Sbjct: 15  KQPPKRFIKNQIPDSILGDASLNAAISILPSIYHFEVHKCVWRIKT--SNAKRIALQLPE 74

Query: 89  GLLMYSLSLSDIFRTFASASDVFVLADVTYGACCVDDLSAAALGADLLIHYGHSCLVPVD 148
           GLLMY+L+LSDIF +FA AS  FVL DVTYGACCVDD SA ALGADLLIHYGHSCLVP+D
Sbjct: 75  GLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPID 134

Query: 149 NTVIPCLYVFVEIGIDVSKLVKTIETNDLGS-RSLAVAGTIQFSGAIRGAKIELERLGFR 208
           +T IPCLYVFVEI IDV  L+ TI  N     +++ +AGTIQF+ AIR  K ELE+ GF 
Sbjct: 135 STKIPCLYVFVEIQIDVKCLLNTIHLNLASDVKNIILAGTIQFTSAIRAVKPELEKQGFN 194

Query: 209 VLVPQAKPLSAGEVLGCTAPRVSKKDFGGIVDDEGEDLVIVFVADGRFHLEAFMIANPEV 268
           VL+PQ+KPLSAGEVLGCTAP+V   +       + +D V+VFVADGRFHLEAFMIANP++
Sbjct: 195 VLIPQSKPLSAGEVLGCTAPKVKTVE-------DCKDQVLVFVADGRFHLEAFMIANPKI 254

Query: 269 KAFRYDPYLGRLFLEEYDHKGMKSSRMKAVLKAREAKSWGVVLGTLGRQGNPKSLERLER 328
           KAFRYDPYLG+LFLEEYDHKGM+ +RM+A+ +AREAK+WG+VLGTLGRQGNPK LERLE+
Sbjct: 255 KAFRYDPYLGKLFLEEYDHKGMRETRMRAIARAREAKTWGIVLGTLGRQGNPKILERLEK 314

Query: 329 KMEEKGMSWTVVLMSEISPTRIALFEDSIDAWIQIACPRLSIDWGEAFHRPLLNPFEAEI 388
           KM EKG+  TVVLMSE+SPTR+ALFEDS+DAW+QIACPRLSIDWGEAF +PLL  FEAEI
Sbjct: 315 KMMEKGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEI 374

Query: 389 ALGLIRGWWERD-----NSDVGVDCGRNSGSCGCDNE-------ANKGGV--DYPMDYYA 448
           ALG IRGWWE D     NS  G        SC C NE        N G +  DYPMDYYA
Sbjct: 375 ALGFIRGWWENDSSSRVNSSSGCCKEDKETSCACKNENVKDDKKDNDGALDGDYPMDYYA 434

Query: 449 QDGGEWNSSYTKGKNRPSRRN 455
           Q+GGEWNSSY K  +RP RRN
Sbjct: 435 QEGGEWNSSYLKKSSRPIRRN 446

		  

BLAST of Spo23965.1 vs. TAIR (Arabidopsis)
Match: AT3G59630.1 (diphthamide synthesis DPH2 family protein)

HSP 1 Score: 67.4 bits (163), Expect = 2.700e-11
Identity = 39/113 (34.51%), Postives = 68/113 (60.18%), Query Frame = 1

		  

Query: 63  FEVHKSVHRILSLSSPSPRVALQLPEGLLMYSLSLSDIFRT---FASASDV--FVLADVT 122
           +E++++   I+S S    R+ALQ P+ LL  S  +    ++     +  +V  FV+AD T
Sbjct: 9   YEINRTAEFIISKSFT--RIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTT 68

Query: 123 YGACCVDDLSAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVEIGIDVSKLVK 171
           YG+CC+D++ A  + ++ ++HYG +CL P   +V+P  +VF +  I+VS  VK
Sbjct: 69  YGSCCIDEVGALHIDSECVVHYGQTCLSP--TSVLPAFFVFGKASINVSSCVK 117