Spo21282.1 (mRNA)

Overview
NameSpo21282.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionPeroxisomal (S)-2-hydroxy-acid oxidase
LocationSpoScf_01013 : 108665 .. 113711 (+)
Sequence length965
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTACTCTTCCATGGCAGACATTAAAACTCTCTACTCTAATTAATCAAATGTTCATCTTCTCGCTAAACGTAATTTCTGAATTGATGTCCAATAATTCCCCAGCAAGAAGGTATTTTGTTCTTTTTGTTGTGATCAATTAGGCTTTAATTTTGAAATTAGGCTTTCGCAATTATTGAACCAATTACAAATTGAACCAACAAGTAAATTTTGATTGCGAAATTAGTAAATTTTGATTGCAAAATTGTTAAATTTTGATTCCGAAATTGTTAAACTTGGATTTGCTCATGTAGAATTTCGTGGGGGTTTAAGCCCTGTTGTTGTTGTATTATTTTGGTTTGTTAATTTTAGGAAATCAAAGTGATTTTTCAGTGTAAAAATGGCTAATTTTCTTGCTTTTTGTTTTGGGTTTTTTTTTCCTGGTAATTTCTTGCATAGTTGTTCACCTGTGAATTTGAAAGCATTTGGTCTCTGGTGGTGCTCACACTGTCGTCTTTTTAATTGTGAATGGAGCAATTTTAGTACAGAAATCAGTAGGTGTAGTATTTTAAAAACTGATGCATCAAGTTTGTAAGTAATCCAAGATCTGTGTGAATTATCTAAACTAATGCCTGTGATTTACCTTTTATAATGTTTGATCATTTATGGTTATATATTGTACCCTTCAGCTCCAGGTCTTGTTAATTGCACATTAGCAATGGAGCTGTAAAGCATAAACTTCACGCAATTAAAGAAACTCCCACAAATGTATCTCATGTCAATTGTGGATCATTGGCCAAACCCTTGAAGTTATATTGTTCTTCTGTTGCGGAGTAAGCTAGTTCTCTTTTAGTCTTTCCTATTCAAAATGGAAATATATCATTTGGATCAGTTGGTTTTTGGTTTTTTCGTTGATTGTGGAGTAAGCCCTGTTGTTGTAATATATCAAATGTATCTCATGTCAATTGTGGATCAATGGCCAAACCCATGATGTTTTTGGTTGATGATGCTGTTTGGCTGATTGTTTTTTCCCCCTGGTGTTGTGGTCTGATATGTCTGCTTGTTGTAGCAAAGTTTCTGATCTAAAATGAAGAAGTTGTTATTGCCGCCGCTGCTGCCGAAGTAGTTGCATTGGCAAGGGCAACAGCCAAGCTTGCAAAGGATGCCGCACTGATGGCCGTTAATAGTCAGCCGACAAAGCACTTGTGAGTACCCATTCACGGTGGGTGAAAGCAGTAGCAGCAGATACTTGCGCACCCATCGTTAGGATGCTTTGTGAGCTGATTCCGAGCTGTCGTTATTCGTTTCTTTTAAGGTATTAATATTGCCTATTAGCTCAACTGGTAGACCTTTGTGCGATTGCACAATGGTGTAGGTTCGAATCCTACATAGGCAACTCTTTGTATTTTGTTATGATATCAAAATGAGAAAATTTTTAAAAACATTTGCTCATTTTTGTAAAATATCTAAATGAAATAAAAAATTTTAAGTTAAGAAATTAAAAATTTTAAAAATATTTCCTCATTTGTGTAAACAATAAAAAAATGAGCAAATTTTTTGAATGTTGAAATATGAGATTTTAGAATATTTGTTCATTAAGACACAGTGAGCAAAGTTTTGAATATTTAGAAACCCGAACTTTAAAATAAAAGTAATGAGCAAAAATATTTGCTCATTTGTGTAAAGTATAAAAAAATGAGCAATTGTTTTGAGTATTAAGTAATCTTAGATATTAAAATATTTGCTCATTTAGACATAATGAGCAAAAGTTTTGAATATTAACTTTAAATTTTTAAAAAAACTATAAAAATTGAACAATTTTTTTGAATATTAAGTAATCTGAGATTTTAAAATATTAGCTCTGATAAGCGTGTTTTCCGTCGTTGATAGAAAACCACCTATGCAAACAATATTTATAAAACCACTTCAACTACCGGCAAGCGGTAAGCAAAGGGATCGTCCCAAAGAAACGGTGAATTATTGAGTTGGAAGTCAATCTAGTTCGATCAAGGATTTGTTTTGAATTGTCAATCGTGCCCAACAACCGTGCGATCGCACGACTGGAACTTGGCTAATTCTCATAAAATCCTCGTGTGCTTGCATAGGAATTCAGTGCGAGCGCACAGAAATTCCGTGCGAGCGCACACTTCTGTGGTGCGAACGCACAACATCCCTTGGAGTTCCTGCATCAAATTTCCGGATTCGTGCGAACGCACACTTTGGGGCACGAATGCACATATTGCCTTGACCTTGGAGACAACTTTGTAGACCCGTGCGAACGCACGTAAATTCCGTGCGATCGCACAGGACCTGTTCGAGCAATTCTCAGTTATTTTCCATCATTTTCAACATTTTTACCTGAAAAGTGCAAAGATGGTATAAGGGGATAAAAATGCAGCAAAACTATATAAAACTATCAAAAAAGCATAATAAAACTCTATAAAATCCTAAGCCTAGAGCGACATAAATTCCGCTCATCATGCTCATTTAGACACAATAAGCAAAAGTTTTGAATATTAACAAATACGAACTTCAAAAATTTTAAAAATGAGCAAAAATATTTGCTCATTTGTGTAAACTATAAGAAATGAGCAAAAATATTTGCTCATTTGTGTAAATTAAAAAAAATGAGCAAATTTTTTGAATGTTAAGTAATCTGATATTTTAAAATATTTTCTCATTTAGACACAATGAGCAAAATCTTTGAACATTACCAAACATGAACTTTAAAATTTTAAAAATGAGCAAAAATATTTGCTCATTTGGGTAAACTATAAAAAATAAGCAAATTTATTTGCCCATTTAGACACGATGAGCAAAAGTTTTCAATATTTGCAAATCTGAACTTTAAAATTTTAAAAATGAGCAAAAGTATTTGCTCATTTGTGTAAACTATAAAAAATGAGCAAATTTATTTGCTCATTTAGTAACGATGAGCAAAAGTTTTCAATATTTACAAATCCGAACTTTAAAGTATTTGTAGGGGGGGGGGGTTTAAATATTTTAGCAGAAGATGGAGATCATGAATGTGAACGAGAATGACGCCAATGCAAAGCAAAAATTGCCAAAGATGGTGTATGACTACTATGCATCTGGTGCAGAAGACCAATGGACTCTGGCTGAGAACAGAAATGCTTTCTCCAAGATCCTATAAGACTGCCTTACAAGTTTGTTTCCCTTTATGCATATGTTTCTGTTTCTGTTTCTGTTTGTTCGTAGTGGAGTAATAAATATCCGCATTAATACAGGGTTCACCCACGTATCCTTATTGATGTAACCAACATCGACATGACGACAACTATCTTGGGATTCAAGATCTCAATGCCTATCATGATAGCCCCAACTGTGGATGGCCTTTTGACATTTCTTAGTGCACTGCTGGGGTATAGTGCTTACCATGTTACCATGCTTGTTATCCCTATCTCAATTATTTTTCGGTGGACCTTGTACTTGGATCACACTCTTAAACGCAACGCCAAATTGGCCTTTATCTTCAATTTTTTTGTTGGCTAGGTCTCTTTGAATTCCATGTCAGAGCTTCTGAGGTTAACCAAAATTGTTCTCAGTAATTCTTTCATGGAATAGATCAGTGATTATTTTCAGCTGTTGTAGGTGTTGTGACTCGGATATAGTCATGTATGTATAACCATTTACCCTGCAGTTGGCATTTTTTTTCCCTTTTACTTGTTAACGAATAGAGACTGTTCGGGTGGTTTCACCGTCCATCATACAGTACTCTTGATGAGTTTACATTAGAGTTTCAGCATTTACTTGTGTAGAATGTAAACTGGTGTCACAATACTTGGTATAATCTAAGTAGTCTTATAAGCTTACAGAATCCAGCATGTACTCTTATTAGCTGTTACTCAATTAAGCATGCTTTTGAGATTGAATTTTCTTGATGTTTCTATATTCAGGTATACAAGGACAGGAATGTAGTTGCTCAACTTATGAGAAGAGCTGAGAGGGCTGGCTTCAAGGCCATTGCCCTTACTGTTGACACACCCCGTTTGGGACGTAGAGAAGCTGATATCAAGAACAGGTATGAAACACATTCTCCTGTTTCCAAAGGAATGAGTCAGGGGTCTTTTGTGATGGTTTATTTATATTTGGTTTTTTAATTTTCAGGTTTGTTTTACCACCTTTTCTAACATTGAAGAACTTTGAGGGTATTGACCTGGGCAAGATGGACAAAGTAAATGTTTAACAATACCAAGATGATTGGAACCAAAGAACTCTTATGTTATTATGTAAACTATATCTAATCTATTTGATATTATTATGCAGGCAAATGACTCTGGCCTTGAATCATATGTCGCTGGGCAGATTGATCGATCCCTGAGCTGGAGGGTAATGTGGGATGGTATTTGAACTTTGTTTGTACTTCTCTGTGGTTGGAAACTATTTCTAGGAGACAGATTAATTTCAAGATAACTTGAGATTAGTCATCTGATCAACGTGTTTTTACTTGGAATTAAACAGGATGTTACGTGGCTTCAGACAATCACTAGCCTCCCCATCCTTGTGAAGGGTGTAATTACATCTGAGGATGGTAAGATTATTGGGCTTAATTTTTCATGAATTTATACAAATCTTGGTCAGTTATCTTATATTTCATATCTTTTTAGCAAGACTGGCCGTTCAACAGGGGCAGTTGGATCCATTGTATCCAACCATAGAGCTCGCCAACTTGATTATGTTCCTGCTACTATAATGGCTCTTGAAGAGGTAAACTGTCTTTTTTTTTCTCAATATATAATCCTAGGGAGGTTGTAATATAAGTGTTTAATTGGTGAAAACTACCTGAACTCTTCAGGTTGTGAAAGCAACACAAGGTCGCATTCTTGTTTTCTGGGATGGTGGTGTTCATCGTGGAACTGACATTTTCAAAGCATTGGCATTGGGTGCCGCTGGAGTATTTGTAAGTAGAATCATATTGCATTGAGATTTTTATTTGGGTTTTTTTGGTACTGATTCAATGATGGTGTTGTGTACTCAGATTGGAAGGCCCGTGGTGTTTTCCCTGGCTCTAGAAGGAGAGGCTAGTGTTAAGAAAGTATAAGAAATAAG

mRNA sequence

ACTACTCTTCCATGGCAGACATTAAAACTCTCTACTCTAATTAATCAAATGTTCATCTTCTCGCTAAACGTAATTTCTGAATTGATGTCCAATAATTCCCCAGCAAGAAGCTCCAGGTCTTAAGTTGTTATTGCCGCCGCTGCTGCCGAAGTAGTTGCATTGGCAAGGGCAACAGCCAAGCTTGCAAAGGATGCCGCACTGATGGCCGTTAATAGTCAGCCGACAAAGCACTTGGTTCACCCACGTATCCTTATTGATGTAACCAACATCGACATGACGACAACTATCTTGGGATTCAAGATCTCAATGCCTATCATGATAGCCCCAACTGTGGATGGCCTTTTGACATTTCTTAGTGCACTGCTGGGGTATAGTGCTTACCATGTATACAAGGACAGGAATGTAGTTGCTCAACTTATGAGAAGAGCTGAGAGGGCTGGCTTCAAGGCCATTGCCCTTACTGTTGACACACCCCGTTTGGGACGTAGAGAAGCTGATATCAAGAACAGGTTTGTTTTACCACCTTTTCTAACATTGAAGAACTTTGAGGGTATTGACCTGGGCAAGATGGACAAAGCAAATGACTCTGGCCTTGAATCATATGTCGCTGGGCAGATTGATCGATCCCTGAGCTGGAGGGATGTTACGTGGCTTCAGACAATCACTAGCCTCCCCATCCTTGTGAAGGGTGTAATTACATCTGAGGATGGGGCAGTTGGATCCATTGTATCCAACCATAGAGCTCGCCAACTTGATTATGTTCCTGCTACTATAATGGCTCTTGAAGAGGTTGTGAAAGCAACACAAGGTCGCATTCTTGTTTTCTGGGATGGTGGTGTTCATCGTGGAACTGACATTTTCAAAGCATTGGCATTGGGTGCCGCTGGAGTATTTATTGGAAGGCCCGTGGTGTTTTCCCTGGCTCTAGAAGGAGAGGCTAGTGTTAAGAAAGTATAAGAAATAAG

Coding sequence (CDS)

ATGGCCGTTAATAGTCAGCCGACAAAGCACTTGGTTCACCCACGTATCCTTATTGATGTAACCAACATCGACATGACGACAACTATCTTGGGATTCAAGATCTCAATGCCTATCATGATAGCCCCAACTGTGGATGGCCTTTTGACATTTCTTAGTGCACTGCTGGGGTATAGTGCTTACCATGTATACAAGGACAGGAATGTAGTTGCTCAACTTATGAGAAGAGCTGAGAGGGCTGGCTTCAAGGCCATTGCCCTTACTGTTGACACACCCCGTTTGGGACGTAGAGAAGCTGATATCAAGAACAGGTTTGTTTTACCACCTTTTCTAACATTGAAGAACTTTGAGGGTATTGACCTGGGCAAGATGGACAAAGCAAATGACTCTGGCCTTGAATCATATGTCGCTGGGCAGATTGATCGATCCCTGAGCTGGAGGGATGTTACGTGGCTTCAGACAATCACTAGCCTCCCCATCCTTGTGAAGGGTGTAATTACATCTGAGGATGGGGCAGTTGGATCCATTGTATCCAACCATAGAGCTCGCCAACTTGATTATGTTCCTGCTACTATAATGGCTCTTGAAGAGGTTGTGAAAGCAACACAAGGTCGCATTCTTGTTTTCTGGGATGGTGGTGTTCATCGTGGAACTGACATTTTCAAAGCATTGGCATTGGGTGCCGCTGGAGTATTTATTGGAAGGCCCGTGGTGTTTTCCCTGGCTCTAGAAGGAGAGGCTAGTGTTAAGAAAGTATAA

Protein sequence

MAVNSQPTKHLVHPRILIDVTNIDMTTTILGFKISMPIMIAPTVDGLLTFLSALLGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo21282Spo21282gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo21282.1Spo21282.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21282.1.exon.1Spo21282.1.exon.1exon
Spo21282.1.exon.2Spo21282.1.exon.2exon
Spo21282.1.exon.3Spo21282.1.exon.3exon
Spo21282.1.exon.4Spo21282.1.exon.4exon
Spo21282.1.exon.5Spo21282.1.exon.5exon
Spo21282.1.exon.6Spo21282.1.exon.6exon
Spo21282.1.exon.7Spo21282.1.exon.7exon
Spo21282.1.exon.8Spo21282.1.exon.8exon
Spo21282.1.exon.9Spo21282.1.exon.9exon
Spo21282.1.exon.10Spo21282.1.exon.10exon
Spo21282.1.exon.11Spo21282.1.exon.11exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21282.1.utr5p.1Spo21282.1.utr5p.1five_prime_UTR
Spo21282.1.utr5p.2Spo21282.1.utr5p.2five_prime_UTR
Spo21282.1.utr5p.3Spo21282.1.utr5p.3five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21282.1.CDS.1Spo21282.1.CDS.1CDS
Spo21282.1.CDS.2Spo21282.1.CDS.2CDS
Spo21282.1.CDS.3Spo21282.1.CDS.3CDS
Spo21282.1.CDS.4Spo21282.1.CDS.4CDS
Spo21282.1.CDS.5Spo21282.1.CDS.5CDS
Spo21282.1.CDS.6Spo21282.1.CDS.6CDS
Spo21282.1.CDS.7Spo21282.1.CDS.7CDS
Spo21282.1.CDS.8Spo21282.1.CDS.8CDS
Spo21282.1.CDS.9Spo21282.1.CDS.9CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21282.1.utr3p.1Spo21282.1.utr3p.1three_prime_UTR


Homology
[Close]
BLAST of Spo21282.1 vs. NCBI nr
Match: gi|641831439|gb|KDO50497.1| (hypothetical protein CISIN_1g017492mg [Citrus sinensis])

HSP 1 Score: 362.8 bits (930), Expect = 4.700e-97
Identity = 192/255 (75.29%), Postives = 209/255 (81.96%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------DGLLTFLSALLGYSAYHVY 70
           L  PRILIDV+ IDM TT+LGFKISMPIMIAPT        +G      A        VY
Sbjct: 47  LFRPRILIDVSKIDMNTTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMVY 106

Query: 71  KDRNVVAQLMRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKM 130
           KDRNVVAQL+RRAERAGFKAIALTVDTPRLGRREADIKNRF LPPFLTLKNF+G+DLGKM
Sbjct: 107 KDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFQGLDLGKM 166

Query: 131 DKANDSGLESYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSED-------GAVGSIV 190
           D+ANDSGL +YVAGQIDRSLSW+DV WLQTIT LPILVKGV+T+ED       GA G IV
Sbjct: 167 DEANDSGLAAYVAGQIDRSLSWKDVKWLQTITKLPILVKGVLTAEDARIAVQAGAAGIIV 226

Query: 191 SNHRARQLDYVPATIMALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPV 250
           SNH ARQLDYVPATIMALEEVVKATQGRI VF DGGV RGTD+FKALALGA+G+FIGRPV
Sbjct: 227 SNHGARQLDYVPATIMALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPV 286

Query: 251 VFSLALEGEASVKKV 252
           V+SLA EGE  V++V
Sbjct: 287 VYSLAAEGEKGVRRV 301
[Close]
BLAST of Spo21282.1 vs. NCBI nr
Match: gi|902183035|gb|KNA10032.1| (hypothetical protein SOVF_148120 [Spinacia oleracea])

HSP 1 Score: 352.4 bits (903), Expect = 6.300e-94
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 1 

		  

Query: 73  MRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLE 132
           MRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLE
Sbjct: 1   MRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLE 60

Query: 133 SYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIM 192
           SYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIM
Sbjct: 61  SYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIM 120

Query: 193 ALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           ALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV
Sbjct: 121 ALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 179
[Close]
BLAST of Spo21282.1 vs. NCBI nr
Match: gi|7431428|pir||T10242 ((S)-2-hydroxy-acid oxidase (EC 1.1.3.15) - cucurbit)

HSP 1 Score: 351.7 bits (901), Expect = 1.100e-93
Identity = 192/281 (68.33%), Postives = 214/281 (76.16%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPT-------------------VDGLLTFL 70
           L  PRILIDV+ IDM+TT+LGFKISMPIMIAPT                     G  T L
Sbjct: 46  LFRPRILIDVSKIDMSTTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTTTTL 105

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYKDRNVVAQL+RRAE+AGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKNRF LPPFLTLKNFEG+DLGKMD+A+DSGL SYVAGQIDR+LSW+DV WLQTIT L
Sbjct: 166 ADIKNRFTLPPFLTLKNFEGLDLGKMDQADDSGLASYVAGQIDRTLSWQDVKWLQTITKL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGV+T+ED       GA G IVSNH ARQLDYVPATIMALEEVVKA +G + VF D
Sbjct: 226 PILVKGVLTAEDTRIAVQSGAAGIIVSNHGARQLDYVPATIMALEEVVKAARGEVPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+G+FIGRPVVFSLA EGEA V+KV
Sbjct: 286 GGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKV 326
[Close]
BLAST of Spo21282.1 vs. NCBI nr
Match: gi|189418957|gb|ACD93720.1| (glycolate oxidase [Mikania micrantha])

HSP 1 Score: 351.7 bits (901), Expect = 1.100e-93
Identity = 195/281 (69.40%), Postives = 211/281 (75.09%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------------------DGLLTFL 70
           L  PRILIDV+ I MTTTILGFKISMPIM+APT                     G +  L
Sbjct: 46  LFRPRILIDVSKIVMTTTILGFKISMPIMVAPTAMQKMAHPEGEYATARAASSAGTIMTL 105

Query: 71  SALLGYSA--------------YHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+    S                +VYKDRNVVAQL+RRAERAGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEAASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKNRF LPPFLTLKNFEG+DLGKMD+ANDSGL SYVAGQIDR+LSW+DV WLQTIT +
Sbjct: 166 ADIKNRFTLPPFLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKM 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGVIT+ED       GA G IVSNH ARQLDYVPATI ALEEVVKA QGR+ VF D
Sbjct: 226 PILVKGVITAEDTRLAIQAGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRVPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGAAG+FIGRPVV SLA EGEA V+KV
Sbjct: 286 GGVRRGTDVFKALALGAAGIFIGRPVVLSLAAEGEAGVRKV 326
[Close]
BLAST of Spo21282.1 vs. NCBI nr
Match: gi|734350507|gb|KHN12454.1| (Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Glycine soja])

HSP 1 Score: 350.5 bits (898), Expect = 2.400e-93
Identity = 185/255 (72.55%), Postives = 206/255 (80.78%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------DGLLTFLSALLGYSAYHVY 70
           L  PRIL+DV+ ID+T T+LGFKISMPIMIAPT        +G L    A        V+
Sbjct: 47  LFRPRILVDVSKIDLTATVLGFKISMPIMIAPTAMQKMAHPEGELATARAASAAGTIMVF 106

Query: 71  KDRNVVAQLMRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKM 130
           KDRNVVAQL+RRAERAGFKAIALTVDTP LGRREADIKNRF LPP L LKNFEG+DLGK+
Sbjct: 107 KDRNVVAQLVRRAERAGFKAIALTVDTPILGRREADIKNRFTLPPNLVLKNFEGLDLGKL 166

Query: 131 DKANDSGLESYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSED-------GAVGSIV 190
           DK +DSGL SYVAGQIDRSL+W+D+ WLQ+ITSLPILVKGV+T+ED       GA G IV
Sbjct: 167 DKTSDSGLASYVAGQIDRSLNWKDIKWLQSITSLPILVKGVLTAEDTRLAIQAGAAGIIV 226

Query: 191 SNHRARQLDYVPATIMALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPV 250
           SNH ARQLDYVPATIMALEEVVKA QG+I VF DGG+ RGTD+FKALALGAAGVFIGRPV
Sbjct: 227 SNHGARQLDYVPATIMALEEVVKAAQGKIPVFLDGGIRRGTDVFKALALGAAGVFIGRPV 286

Query: 251 VFSLALEGEASVKKV 252
           VFSLA +GE  V+KV
Sbjct: 287 VFSLAADGETGVRKV 301
[Close]
BLAST of Spo21282.1 vs. UniProtKB/TrEMBL
Match: A0A067EH02_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017492mg PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.300e-97
Identity = 192/255 (75.29%), Postives = 209/255 (81.96%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------DGLLTFLSALLGYSAYHVY 70
           L  PRILIDV+ IDM TT+LGFKISMPIMIAPT        +G      A        VY
Sbjct: 47  LFRPRILIDVSKIDMNTTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMVY 106

Query: 71  KDRNVVAQLMRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKM 130
           KDRNVVAQL+RRAERAGFKAIALTVDTPRLGRREADIKNRF LPPFLTLKNF+G+DLGKM
Sbjct: 107 KDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFQGLDLGKM 166

Query: 131 DKANDSGLESYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSED-------GAVGSIV 190
           D+ANDSGL +YVAGQIDRSLSW+DV WLQTIT LPILVKGV+T+ED       GA G IV
Sbjct: 167 DEANDSGLAAYVAGQIDRSLSWKDVKWLQTITKLPILVKGVLTAEDARIAVQAGAAGIIV 226

Query: 191 SNHRARQLDYVPATIMALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPV 250
           SNH ARQLDYVPATIMALEEVVKATQGRI VF DGGV RGTD+FKALALGA+G+FIGRPV
Sbjct: 227 SNHGARQLDYVPATIMALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPV 286

Query: 251 VFSLALEGEASVKKV 252
           V+SLA EGE  V++V
Sbjct: 287 VYSLAAEGEKGVRRV 301
[Close]
BLAST of Spo21282.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QRV9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_148120 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.400e-94
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 1 

		  

Query: 73  MRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLE 132
           MRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLE
Sbjct: 1   MRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLE 60

Query: 133 SYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIM 192
           SYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIM
Sbjct: 61  SYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSEDGAVGSIVSNHRARQLDYVPATIM 120

Query: 193 ALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           ALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV
Sbjct: 121 ALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 179
[Close]
BLAST of Spo21282.1 vs. UniProtKB/TrEMBL
Match: A0A0E0Q440_ORYRU (Uncharacterized protein OS=Oryza rufipogon PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.400e-94
Identity = 189/255 (74.12%), Postives = 206/255 (80.78%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------DGLLTFLSALLGYSAYHVY 70
           L  PRILIDV+ IDM TT+LGFKISMPIMIAP+        DG      A        VY
Sbjct: 47  LFRPRILIDVSKIDMATTVLGFKISMPIMIAPSAMQKMAHPDGEYATARAASAAGTIMVY 106

Query: 71  KDRNVVAQLMRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKM 130
           KDR VV QL+RRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEG++LGKM
Sbjct: 107 KDRRVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGLELGKM 166

Query: 131 DKANDSGLESYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSED-------GAVGSIV 190
           D+A+DSGL SYVAGQIDR+LSW+DV WLQTIT+LPILVKGVIT+ED       GA G IV
Sbjct: 167 DQASDSGLASYVAGQIDRTLSWKDVKWLQTITTLPILVKGVITAEDTRLAVENGAAGIIV 226

Query: 191 SNHRARQLDYVPATIMALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPV 250
           SNH ARQLDYVPATI ALEEVVKA +G++ VF DGGV RGTD+FKALALGAAGVFIGRPV
Sbjct: 227 SNHGARQLDYVPATISALEEVVKAARGQLPVFLDGGVRRGTDVFKALALGAAGVFIGRPV 286

Query: 251 VFSLALEGEASVKKV 252
           VFSLA  GEA V+ V
Sbjct: 287 VFSLAAAGEAGVRNV 301
[Close]
BLAST of Spo21282.1 vs. UniProtKB/TrEMBL
Match: A0A0E0HWI0_ORYNI (Uncharacterized protein OS=Oryza nivara PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.400e-94
Identity = 189/255 (74.12%), Postives = 206/255 (80.78%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------DGLLTFLSALLGYSAYHVY 70
           L  PRILIDV+ IDM TT+LGFKISMPIMIAP+        DG      A        VY
Sbjct: 47  LFRPRILIDVSKIDMATTVLGFKISMPIMIAPSAMQKMAHPDGEYATARAASSAGTIMVY 106

Query: 71  KDRNVVAQLMRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKM 130
           KDR VV QL+RRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEG++LGKM
Sbjct: 107 KDRRVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGLELGKM 166

Query: 131 DKANDSGLESYVAGQIDRSLSWRDVTWLQTITSLPILVKGVITSED-------GAVGSIV 190
           D+A+DSGL SYVAGQIDR+LSW+DV WLQTIT+LPILVKGVIT+ED       GA G IV
Sbjct: 167 DQASDSGLASYVAGQIDRTLSWKDVKWLQTITTLPILVKGVITAEDTRLAVENGAAGIIV 226

Query: 191 SNHRARQLDYVPATIMALEEVVKATQGRILVFWDGGVHRGTDIFKALALGAAGVFIGRPV 250
           SNH ARQLDYVPATI ALEEVVKA +G++ VF DGGV RGTD+FKALALGAAGVFIGRPV
Sbjct: 227 SNHGARQLDYVPATISALEEVVKAARGQLPVFLDGGVRRGTDVFKALALGAAGVFIGRPV 286

Query: 251 VFSLALEGEASVKKV 252
           VFSLA  GEA V+ V
Sbjct: 287 VFSLAAAGEAGVRNV 301
[Close]
BLAST of Spo21282.1 vs. UniProtKB/TrEMBL
Match: Q39640_9ROSI (Glycolate oxidase OS=Cucurbita cv. Kurokawa Amakuri PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 7.500e-94
Identity = 192/281 (68.33%), Postives = 214/281 (76.16%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPT-------------------VDGLLTFL 70
           L  PRILIDV+ IDM+TT+LGFKISMPIMIAPT                     G  T L
Sbjct: 46  LFRPRILIDVSKIDMSTTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTTTTL 105

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYKDRNVVAQL+RRAE+AGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKNRF LPPFLTLKNFEG+DLGKMD+A+DSGL SYVAGQIDR+LSW+DV WLQTIT L
Sbjct: 166 ADIKNRFTLPPFLTLKNFEGLDLGKMDQADDSGLASYVAGQIDRTLSWQDVKWLQTITKL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGV+T+ED       GA G IVSNH ARQLDYVPATIMALEEVVKA +G + VF D
Sbjct: 226 PILVKGVLTAEDTRIAVQSGAAGIIVSNHGARQLDYVPATIMALEEVVKAARGEVPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+G+FIGRPVVFSLA EGEA V+KV
Sbjct: 286 GGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKV 326
[Close]
BLAST of Spo21282.1 vs. ExPASy Swiss-Prot
Match: GOX_SPIOL (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.000e-95
Identity = 200/281 (71.17%), Postives = 212/281 (75.44%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILG--------------FKISMP-----IMIAPTVDGLLTFL 70
           L  PRILIDVTNIDMTTTILG               K++ P        A +  G +  L
Sbjct: 46  LFRPRILIDVTNIDMTTTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTL 105

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYKDRNVVAQL+RRAERAGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGL SYVAGQIDRSLSW+DV WLQTITSL
Sbjct: 166 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLSSYVAGQIDRSLSWKDVAWLQTITSL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGVIT+ED       GA G IVSNH ARQLDYVPATIMALEEVVKA QGRI VF D
Sbjct: 226 PILVKGVITAEDARLAVQHGAAGIIVSNHGARQLDYVPATIMALEEVVKAAQGRIPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGAAGVFIGRPVVFSLA EGEA VKKV
Sbjct: 286 GGVRRGTDVFKALALGAAGVFIGRPVVFSLAAEGEAGVKKV 326
[Close]
BLAST of Spo21282.1 vs. ExPASy Swiss-Prot
Match: GLO5_ARATH (Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Arabidopsis thaliana GN=GLO5 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 3.600e-89
Identity = 181/281 (64.41%), Postives = 207/281 (73.67%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPT-------------------VDGLLTFL 70
           L  PRILIDV+ ID++TT+LGF ISMPIMIAPT                     G +  L
Sbjct: 46  LFRPRILIDVSKIDVSTTVLGFNISMPIMIAPTAMQKMAHPDGELATARATSAAGTIMTL 105

Query: 71  SALLGYSAYHV--------------YKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+    S   V              YKDRNVV QL++RAE AGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATCSVEEVASTGPGIRFFQLYVYKDRNVVIQLVKRAEEAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           +DIKNRF LP  LTLKNFEG+DLGK+DK NDSGL SYVAGQ+D+SLSW+D+ WLQ+ITSL
Sbjct: 166 SDIKNRFALPRGLTLKNFEGLDLGKIDKTNDSGLASYVAGQVDQSLSWKDIKWLQSITSL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGVIT+ED       GA G IVSNH ARQLDYVPATI+ALEEVVKA +GRI VF D
Sbjct: 226 PILVKGVITAEDARIAVEYGAAGIIVSNHGARQLDYVPATIVALEEVVKAVEGRIPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+GVF+GRP +FSLA +GEA V+K+
Sbjct: 286 GGVRRGTDVFKALALGASGVFVGRPSLFSLAADGEAGVRKM 326
[Close]
BLAST of Spo21282.1 vs. ExPASy Swiss-Prot
Match: GLO1_ARATH (Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 OS=Arabidopsis thaliana GN=GLO1 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 4.000e-88
Identity = 184/281 (65.48%), Postives = 206/281 (73.31%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILG--------------FKISMP-----IMIAPTVDGLLTFL 70
           L  PRILIDV+ IDMTTT+LG               K++ P        A +  G +  L
Sbjct: 46  LFRPRILIDVSKIDMTTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTL 105

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYK+RNVV QL+RRAERAGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           +DIKNRF LPP LTLKNFEG+DLGKMD+ANDSGL SYVAGQIDR+LSW+DV WLQTIT L
Sbjct: 166 SDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGV+T ED       GA G IVSNH ARQLDYVPATI ALEEVVKATQGRI VF D
Sbjct: 226 PILVKGVLTGEDARIAIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+G+FIGRPVVFSLA EGEA V+KV
Sbjct: 286 GGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKV 326
[Close]
BLAST of Spo21282.1 vs. ExPASy Swiss-Prot
Match: GLO3_ORYSI (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Oryza sativa subsp. indica GN=GLO3 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 6.800e-88
Identity = 179/281 (63.70%), Postives = 203/281 (72.24%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAP-------------------TVDGLLTFL 70
           L  PRILIDV+ I+M T +LGF ISMPIMIAP                   +  G +  L
Sbjct: 47  LFRPRILIDVSRINMATNVLGFNISMPIMIAPSAMQKMAHPEGELATARAASAAGTIMTL 106

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYKDRN+V QL+RRAE AGFKAIALTVDTPRLGRRE
Sbjct: 107 SSWSTSSVEEVNSAAPGIRFFQLYVYKDRNIVRQLVRRAELAGFKAIALTVDTPRLGRRE 166

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKNRF LPP L LKNFE +DLGKMDK NDSGL SYVA Q+DRSLSW DV WLQTITSL
Sbjct: 167 ADIKNRFNLPPHLVLKNFEALDLGKMDKTNDSGLASYVASQVDRSLSWTDVKWLQTITSL 226

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGV+T+ED       GA G IVSNH ARQLDYVPATI  LEEVV+  +GR+ VF D
Sbjct: 227 PILVKGVMTAEDTRLAVESGAAGIIVSNHGARQLDYVPATISCLEEVVREAKGRLPVFLD 286

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+GVFIGRPV+FSLA++GEA V+KV
Sbjct: 287 GGVRRGTDVFKALALGASGVFIGRPVLFSLAVDGEAGVRKV 327
[Close]
BLAST of Spo21282.1 vs. ExPASy Swiss-Prot
Match: GLO3_ORYSJ (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Oryza sativa subsp. japonica GN=GLO3 PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 6.800e-88
Identity = 179/281 (63.70%), Postives = 203/281 (72.24%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAP-------------------TVDGLLTFL 70
           L  PRILIDV+ I+M T +LGF ISMPIMIAP                   +  G +  L
Sbjct: 47  LFRPRILIDVSRINMATNVLGFNISMPIMIAPSAMQKMAHPEGELATARAASAAGTIMTL 106

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYKDRN+V QL+RRAE AGFKAIALTVDTPRLGRRE
Sbjct: 107 SSWSTSSVEEVNSAAPGIRFFQLYVYKDRNIVRQLVRRAELAGFKAIALTVDTPRLGRRE 166

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKNRF LPP L LKNFE +DLGKMDK NDSGL SYVA Q+DRSLSW DV WLQTITSL
Sbjct: 167 ADIKNRFNLPPHLVLKNFEALDLGKMDKTNDSGLASYVASQVDRSLSWTDVKWLQTITSL 226

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGV+T+ED       GA G IVSNH ARQLDYVPATI  LEEVV+  +GR+ VF D
Sbjct: 227 PILVKGVMTAEDTRLAVESGAAGIIVSNHGARQLDYVPATISCLEEVVREAKGRLPVFLD 286

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+GVFIGRPV+FSLA++GEA V+KV
Sbjct: 287 GGVRRGTDVFKALALGASGVFIGRPVLFSLAVDGEAGVRKV 327
[Close]
BLAST of Spo21282.1 vs. TAIR (Arabidopsis)
Match: AT4G18360.1 (Aldolase-type TIM barrel family protein)

HSP 1 Score: 329.3 bits (843), Expect = 2.000e-90
Identity = 181/281 (64.41%), Postives = 207/281 (73.67%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPT-------------------VDGLLTFL 70
           L  PRILIDV+ ID++TT+LGF ISMPIMIAPT                     G +  L
Sbjct: 46  LFRPRILIDVSKIDVSTTVLGFNISMPIMIAPTAMQKMAHPDGELATARATSAAGTIMTL 105

Query: 71  SALLGYSAYHV--------------YKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+    S   V              YKDRNVV QL++RAE AGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATCSVEEVASTGPGIRFFQLYVYKDRNVVIQLVKRAEEAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           +DIKNRF LP  LTLKNFEG+DLGK+DK NDSGL SYVAGQ+D+SLSW+D+ WLQ+ITSL
Sbjct: 166 SDIKNRFALPRGLTLKNFEGLDLGKIDKTNDSGLASYVAGQVDQSLSWKDIKWLQSITSL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGVIT+ED       GA G IVSNH ARQLDYVPATI+ALEEVVKA +GRI VF D
Sbjct: 226 PILVKGVITAEDARIAVEYGAAGIIVSNHGARQLDYVPATIVALEEVVKAVEGRIPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+GVF+GRP +FSLA +GEA V+K+
Sbjct: 286 GGVRRGTDVFKALALGASGVFVGRPSLFSLAADGEAGVRKM 326
[Close]
BLAST of Spo21282.1 vs. TAIR (Arabidopsis)
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein)

HSP 1 Score: 325.9 bits (834), Expect = 2.200e-89
Identity = 184/281 (65.48%), Postives = 206/281 (73.31%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILG--------------FKISMP-----IMIAPTVDGLLTFL 70
           L  PRILIDV+ IDMTTT+LG               K++ P        A +  G +  L
Sbjct: 46  LFRPRILIDVSKIDMTTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTL 105

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYK+RNVV QL+RRAERAGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           +DIKNRF LPP LTLKNFEG+DLGKMD+ANDSGL SYVAGQIDR+LSW+DV WLQTIT L
Sbjct: 166 SDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKL 225

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKGV+T ED       GA G IVSNH ARQLDYVPATI ALEEVVKATQGRI VF D
Sbjct: 226 PILVKGVLTGEDARIAIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLD 285

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA+G+FIGRPVVFSLA EGEA V+KV
Sbjct: 286 GGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKV 326
[Close]
BLAST of Spo21282.1 vs. TAIR (Arabidopsis)
Match: AT3G14415.2 (Aldolase-type TIM barrel family protein)

HSP 1 Score: 312.4 bits (799), Expect = 2.600e-85
Identity = 178/287 (62.02%), Postives = 204/287 (71.08%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILG--------------FKISMP-----IMIAPTVDGLLTFL 70
           L  PRILIDV  IDM TT+LG               K++ P        A +  G +  L
Sbjct: 46  LFRPRILIDVNKIDMATTVLGFKISMPIMVAPTAFQKMAHPDGEYATARAASAAGTIMTL 105

Query: 71  SAL--------------LGYSAYHVYKDRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S+               + +   +VYK+R VV QL+RRAE+AGFKAIALTVDTPRLGRRE
Sbjct: 106 SSWATSSVEEVASTGPGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRE 165

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKA------NDSGLESYVAGQIDRSLSWRDVTWL 190
           +DIKNRF LPP LTLKNFEG+DLGKMD+A      NDSGL SYVAGQIDR+LSW+D+ WL
Sbjct: 166 SDIKNRFTLPPNLTLKNFEGLDLGKMDEASIDQIANDSGLASYVAGQIDRTLSWKDIQWL 225

Query: 191 QTITSLPILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGR 250
           QTIT++PILVKGV+T ED       GA G IVSNH ARQLDYVPATI ALEEVVKATQGR
Sbjct: 226 QTITNMPILVKGVLTGEDARIAIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGR 285

Query: 251 ILVFWDGGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           + VF DGGV RGTD+FKALALGA+G+FIGRPVVF+LA EGEA VKKV
Sbjct: 286 VPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFALAAEGEAGVKKV 332
[Close]
BLAST of Spo21282.1 vs. TAIR (Arabidopsis)
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein)

HSP 1 Score: 242.3 bits (617), Expect = 3.300e-64
Identity = 138/281 (49.11%), Postives = 175/281 (62.28%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTV-------DGLLTFLSALLGYSAYHVY 70
           +  PR+L+DV+NIDM+T++LG+ IS PIMIAPT         G +    A    +   + 
Sbjct: 47  MFRPRVLVDVSNIDMSTSMLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIV 106

Query: 71  K--------------------------DRNVVAQLMRRAERAGFKAIALTVDTPRLGRRE 130
                                       R+V AQ+++RAE+AGFKAI LTVD PRLGRRE
Sbjct: 107 SFMSTCTIEEVASSCNAVRFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRRE 166

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKN+ + P    LKNFEG+   ++     SG+E++ +   D SLSW+D+ WL++IT L
Sbjct: 167 ADIKNKMISP---QLKNFEGLVSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKL 226

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKG++T ED       G  G +VSNH ARQLDY PATI  LEEVV A +GRI V  D
Sbjct: 227 PILVKGLLTREDALKAVEAGVDGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLD 286

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA  V IGRP+V+ LA +GE  VKKV
Sbjct: 287 GGVRRGTDVFKALALGAQAVLIGRPIVYGLAAKGEDGVKKV 324
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BLAST of Spo21282.1 vs. TAIR (Arabidopsis)
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein)

HSP 1 Score: 234.2 bits (596), Expect = 8.900e-62
Identity = 133/281 (47.33%), Postives = 173/281 (61.57%), Query Frame = 1 

		  

Query: 11  LVHPRILIDVTNIDMTTTILGFKISMPIMIAPTVD-------------------GLLTFL 70
           +  PR+L+DV+ IDM+T ILG+ IS PIMIAPT +                     +  +
Sbjct: 47  MFRPRVLVDVSKIDMSTKILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIV 106

Query: 71  SALLGYSAYHVYKDRNVV--------------AQLMRRAERAGFKAIALTVDTPRLGRRE 130
           S +   +   +    N V              AQ+++RAE+AGFKAI LTVD PRLGRRE
Sbjct: 107 SYMSSCTFEEIASSCNAVRFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRRE 166

Query: 131 ADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLESYVAGQIDRSLSWRDVTWLQTITSL 190
           ADIKN+ + P    LKNFEG+   ++  +  SG++++ +   D S SW+D+ WL++IT L
Sbjct: 167 ADIKNKMISP---QLKNFEGLFSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITEL 226

Query: 191 PILVKGVITSED-------GAVGSIVSNHRARQLDYVPATIMALEEVVKATQGRILVFWD 250
           PILVKG++T ED       G  G IVSNH  RQLDY PATI  LEEVV+  +GRI V  D
Sbjct: 227 PILVKGILTREDALKAVEAGVDGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLD 286

Query: 251 GGVHRGTDIFKALALGAAGVFIGRPVVFSLALEGEASVKKV 252
           GGV RGTD+FKALALGA  V IGRP+++ LA +GE  VKKV
Sbjct: 287 GGVRRGTDVFKALALGAQAVLIGRPIIYGLAAKGEDGVKKV 324
The following BLAST results are available for this feature:
BLAST of Spo21282.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|641831439|gb|KDO50497.1|4.7e-9775.2hypothetical protein CISIN_1g0... [more]
gi|902183035|gb|KNA10032.1|6.3e-94100.hypothetical protein SOVF_1481... [more]
gi|7431428|pir||T102421.1e-9368.3(S)-2-hydroxy-acid oxidase (EC... [more]
gi|189418957|gb|ACD93720.1|1.1e-9369.4glycolate oxidase [Mikania mic... [more]
gi|734350507|gb|KHN12454.1|2.4e-9372.5Peroxisomal (S)-2-hydroxy-acid... [more]
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BLAST of Spo21282.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A067EH02_CITSI3.3e-9775.2Uncharacterized protein OS=Cit... [more]
A0A0K9QRV9_SPIOL4.4e-94100.Uncharacterized protein OS=Spi... [more]
A0A0E0Q440_ORYRU4.4e-9474.1Uncharacterized protein OS=Ory... [more]
A0A0E0HWI0_ORYNI4.4e-9474.1Uncharacterized protein OS=Ory... [more]
Q39640_9ROSI7.5e-9468.3Glycolate oxidase OS=Cucurbita... [more]
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BLAST of Spo21282.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
GOX_SPIOL2.0e-9571.1Peroxisomal (S)-2-hydroxy-acid... [more]
GLO5_ARATH3.6e-8964.4Peroxisomal (S)-2-hydroxy-acid... [more]
GLO1_ARATH4.0e-8865.4Peroxisomal (S)-2-hydroxy-acid... [more]
GLO3_ORYSI6.8e-8863.7Peroxisomal (S)-2-hydroxy-acid... [more]
GLO3_ORYSJ6.8e-8863.7Peroxisomal (S)-2-hydroxy-acid... [more]
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BLAST of Spo21282.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT4G18360.12.0e-9064.4Aldolase-type TIM barrel famil... [more]
AT3G14420.12.2e-8965.4Aldolase-type TIM barrel famil... [more]
AT3G14415.22.6e-8562.0Aldolase-type TIM barrel famil... [more]
AT3G14130.13.3e-6449.1Aldolase-type TIM barrel famil... [more]
AT3G14150.18.9e-6247.3Aldolase-type TIM barrel famil... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000262FMN-dependent dehydrogenasePFAMPF01070FMN_dhcoord: 61..251
score: 4.4E-59coord: 11..43
score: 7.
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentPROFILEPS51349FMN_HYDROXY_ACID_DH_2coord: 1..251
score: 49
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70coord: 11..43
score: 6.3E-8coord: 62..251
score: 1.0
NoneNo IPR availablePANTHERPTHR10578S -2-HYDROXY-ACID OXIDASE-RELATEDcoord: 11..251
score: 1.3E
NoneNo IPR availablePANTHERPTHR10578:SF70PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO5coord: 11..251
score: 1.3E
NoneNo IPR availableunknownSSF51395FMN-linked oxidoreductasescoord: 11..250
score: 4.1

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0046487 glyoxylate metabolic process
biological_process GO:0009853 photorespiration
molecular_function GO:0003824 catalytic activity
molecular_function GO:0010181 FMN binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003973 (S)-2-hydroxy-acid oxidase activity