Spo16374 (gene) | SpinachBase

Overview


Name	Spo16374
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	4Fe-4S ferredoxin, iron-sulfur binding protein
Location	SpoScf_02527 : 14687 .. 16555 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTAGTTAGGTTTCGATTAATAGAACAAAGTTGAACTAAAAAGCAATAAAAATAAAACGTATCCTCTTCTTAGATGTTTTCAGAATAATTGCTTTCAGTGAAGTGTATTTATGGTCAATTTCTGTTGCTGTTTTTTTTCTTTTTTCTATCTTCTTTTGTTTCTATCAATAGTTAACTTTCACAGAATTTCTTCAATTGGCTGGTGGCACAAATGCTCACACAATAAAAGGGTTGAAGGAACACAGACTTTTTCAAACCAGGAGTAATATTCTGACCCTTTAAGTGTCCTTATTGGTCTAGTTTTGTCTTTAATTGAAATACTTAACCCTTTATTAAACCCGTTTTGCAGGGAACCCGGATGATGAGATCTTGACCGGTGAAATATCTAGTTCATGCAATTCTCTAATTGGTGGTATGGCATATGGTGGCTATGCTAGGAAGGTACGTACAACTATATTACTGAAATGGCAAGGTGGCCAAATAATGTAAATTTGTGTATAAATTTCTATTTGGAGAAACATATTCTTAATAAGACTTTAAAATGGAAAGATGGCTAAAGAAAACCAGCTTCTACTTATCATCTTGTAGCTTCTTTCTTGTTGTCATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTGCTCTAGAGAATTTTTACATAACCTGTCTGTCTGCACCTCCCTGCTATAAACTGAAAATTCTACATTTATTGGATCCAAAATGAAATGTCAAGTGAACATACAGACATACTGACATAGTCATGTCTGAATATAGAAGAGCTAGGGTTATCTTAAGTAAATGAGCCTGAGTAACTTAGCAGAAAAGCAGTGGGTGAACTTTTCAGACTGCAGCAAATCAAAGTACACATGTATCATAGTGAATCTTTTCAGACTTTTTCCCTATTTCCTAGATCTTCCCTTGTCCCTAAGCATGTTAACCTTCATTACTACTGAAGTCATTCATTCTCCATTAGGTGTACTTATGCTAGATATGACGCGACTACATTATATACACAGAGTACATGGTAATGATGATTACTAAGGCATTTATTGAGTCTGCTTGACATAAGTCATGAGTCTGCTTACTGAGTCTGCTTACTAAGGCATTTGCTGAGTCTGCTTGGCATTTACATAAGTCAGAGTACATGGTAATGATGATTACTAAGGCATTTACATACGACTTGGGTGTTGTAGTTGACATGCTGACCCACTTGGAGGTAATTCTCTTGGGTTTGCTTGAATATTTAAAGGTCTCCGCTAATTGTTTGACAAACGTTAGAATTTCTGTTGCTTTAATCTTTAGTATGCTATGGGATTGCGTTGTAACAGATCAATCCAAGATTTTGTAGGCTTATTGATAAACATTTTCATTGCTCTAACTATAGTGAGAATGTGAATTTGATGGTACTGAAATTTTATCCCTGTACCGTGCTGCAATTATTTGCTTATATTGTCGAGTAATAGCAAAGATTTGCTTCCATTCCGGTTACTCTTTCTTGATATGTACACAGCGTGACTCTCTACTCAGGTACAAAGGTGTGAAGACTCTAGACCTCTAAAAATTGAGTTGAGAGGAAAATGAACAGTGTGATTCGGATGCATACTGTTTGTGCTAGAGCTTTTGGACCTGGTAAAAGTTACAGATACTCAATTTATCTATCTTGGGGTCAATTTGACAGTAACTGGCATATTAGGGAAATGTGTATCTCATGGAGTCATAAGTGTCGTAGGAAAGTTAGACGCATTGAATTTAATTTGAATGCATCTCATACTGAGCCTATGGCCTTTGTAGCAGATAGAGGAAGGTTTAATGACAAGTTCAATAAAAG

mRNA sequence

ATGTTAACTTTCACAGAATTTCTTCAATTGGCTGGTGGCACAAATGCTCACACAATAAAAGGGTTGAAGGAACACAGACTTTTTCAAACCAGGAGGAACCCGGATGATGAGATCTTGACCGGTGAAATATCTAGTTCATGCAATTCTCTAATTGGTGGTATGGCATATGGTGGCTATGCTAGGAAGGTACAAAGGTGTGAAGACTCTAGACCTCTAAAAATTGAGTTGAGAGGAAAATGAACAGTGTGATTCGGATGCATACTGTTTGTGCTAGAGCTTTTGGACCTGATAGAGGAAGGTTTAATGACAAGTTCAATAAAAG

Coding sequence (CDS)

ATGTTAACTTTCACAGAATTTCTTCAATTGGCTGGTGGCACAAATGCTCACACAATAAAAGGGTTGAAGGAACACAGACTTTTTCAAACCAGGAGGAACCCGGATGATGAGATCTTGACCGGTGAAATATCTAGTTCATGCAATTCTCTAATTGGTGGTATGGCATATGGTGGCTATGCTAGGAAGGTACAAAGGTGTGAAGACTCTAGACCTCTAAAAATTGAGTTGAGAGGAAAATGA

Protein sequence

MLTFTEFLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKVQRCEDSRPLKIELRGK

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo16374.1	Spo16374.1	mRNA

Homology

[Close]

BLAST of Spo16374.1 vs. NCBI nr
Match: gi|902086902|gb|KNA03235.1| (hypothetical protein SOVF_211140 [Spinacia oleracea])

HSP 1 Score: 108.6 bits (270), Expect = 5.000e-21
Identity = 50/57 (87.72%), Postives = 54/57 (94.74%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNA T+KGLKEH LFQTRRNPDD ILTGEIS+SCNSLIGG+AYGGYARK+
Sbjct: 309 FLQLAGGTNAQTVKGLKEHGLFQTRRNPDDGILTGEISNSCNSLIGGIAYGGYARKI 365

[Close]

BLAST of Spo16374.1 vs. NCBI nr
Match: gi|731322144|ref|XP_010671730.1| (PREDICTED: uncharacterized protein LOC104888450 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 88.6 bits (218), Expect = 5.400e-15
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLKE  LFQ RRN +D IL  ++S SCNSLI G+AYGGYARK+
Sbjct: 309 FLQLAGGTNAHTVNGLKEKGLFQIRRNLEDGILAEQMSGSCNSLISGIAYGGYARKI 365

[Close]

BLAST of Spo16374.1 vs. NCBI nr
Match: gi|870865205|gb|KMT16272.1| (hypothetical protein BVRB_3g054230 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 88.6 bits (218), Expect = 5.400e-15
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLKE  LFQ RRN +D IL  ++S SCNSLI G+AYGGYARK+
Sbjct: 325 FLQLAGGTNAHTVNGLKEKGLFQIRRNLEDGILAEQMSGSCNSLISGIAYGGYARKI 381

[Close]

BLAST of Spo16374.1 vs. NCBI nr
Match: gi|1021052077|gb|KZN09855.1| (hypothetical protein DCAR_002511 [Daucus carota subsp. sativus])

HSP 1 Score: 75.9 bits (185), Expect = 3.600e-11
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLK+HRLFQT     D+ +    S S N+LIGG+AYGGYARK+
Sbjct: 311 FLQLAGGTNAHTVDGLKKHRLFQTTTTSKDKNVHSS-SPSSNALIGGVAYGGYARKI 366

[Close]

BLAST of Spo16374.1 vs. NCBI nr
Match: gi|596298266|ref|XP_007227463.1| (hypothetical protein PRUPE_ppa006159mg [Prunus persica])

HSP 1 Score: 75.5 bits (184), Expect = 4.700e-11
Identity = 40/60 (66.67%), Postives = 47/60 (78.33%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQT---RRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLK+  LFQT    RN  DE LTG  SSS +++IGG+AYGGYARK+
Sbjct: 320 FLQLAGGTNAHTVDGLKKVGLFQTTSFHRNSRDETLTG-TSSSVHAIIGGVAYGGYARKI 378

[Close]

BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q7L3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_211140 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.500e-21
Identity = 50/57 (87.72%), Postives = 54/57 (94.74%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNA T+KGLKEH LFQTRRNPDD ILTGEIS+SCNSLIGG+AYGGYARK+
Sbjct: 309 FLQLAGGTNAQTVKGLKEHGLFQTRRNPDDGILTGEISNSCNSLIGGIAYGGYARKI 365

[Close]

BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CRP9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g054230 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.700e-15
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLKE  LFQ RRN +D IL  ++S SCNSLI G+AYGGYARK+
Sbjct: 325 FLQLAGGTNAHTVNGLKEKGLFQIRRNLEDGILAEQMSGSCNSLISGIAYGGYARKI 381

[Close]

BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
Match: A0A169WT74_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_002511 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.500e-11
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQTRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLK+HRLFQT     D+ +    S S N+LIGG+AYGGYARK+
Sbjct: 311 FLQLAGGTNAHTVDGLKKHRLFQTTTTSKDKNVHSS-SPSSNALIGGVAYGGYARKI 366

[Close]

BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
Match: M5XRG4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006159mg PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.300e-11
Identity = 40/60 (66.67%), Postives = 47/60 (78.33%), Query Frame = 1


		  
Query: 7   FLQLAGGTNAHTIKGLKEHRLFQT---RRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
           FLQLAGGTNAHT+ GLK+  LFQT    RN  DE LTG  SSS +++IGG+AYGGYARK+
Sbjct: 320 FLQLAGGTNAHTVDGLKKVGLFQTTSFHRNSRDETLTG-TSSSVHAIIGGVAYGGYARKI 378

[Close]

BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
Match: G8E4B6_9ROSI (4Fe-4S ferredoxin iron-sulfur binding protein (Fragment) OS=Dimocarpus longan GN=4Fe-4S FD PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 7.300e-11
Identity = 38/60 (63.33%), Postives = 46/60 (76.67%), Query Frame = 1


		  
Query: 7  FLQLAGGTNAHTIKGLKEHRLFQ---TRRNPDDEILTGEISSSCNSLIGGMAYGGYARKV 64
          FLQLAGGTNAHT+ GLK+  LFQ   T+ + D E LT  + SS +SLIGG+AYGGYARK+
Sbjct: 2  FLQLAGGTNAHTVDGLKKEGLFQTSFTQHSRDAEELTAILLSSSSSLIGGIAYGGYARKI 61

The following BLAST results are available for this feature:

BLAST of Spo16374.1 vs. NCBI nr
BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
BLAST of Spo16374.1 vs. ExPASy Swiss-Prot
BLAST of Spo16374.1 vs. TAIR (Arabidopsis)

BLAST of Spo16374.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902086902\|gb\|KNA03235.1\|	5.0e-21	87.7	hypothetical protein SOVF_2111...	[more]
gi\|731322144\|ref\|XP_010671730.1\|	5.4e-15	71.9	PREDICTED: uncharacterized pro...	[more]
gi\|870865205\|gb\|KMT16272.1\|	5.4e-15	71.9	hypothetical protein BVRB_3g05...	[more]
gi\|1021052077\|gb\|KZN09855.1\|	3.6e-11	64.9	hypothetical protein DCAR_0025...	[more]
gi\|596298266\|ref\|XP_007227463.1\|	4.7e-11	66.6	hypothetical protein PRUPE_ppa...	[more]

BLAST of Spo16374.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q7L3_SPIOL	3.5e-21	87.7	Uncharacterized protein OS=Spi...	[more]
A0A0J8CRP9_BETVU	3.7e-15	71.9	Uncharacterized protein OS=Bet...	[more]
A0A169WT74_DAUCA	2.5e-11	64.9	Uncharacterized protein OS=Dau...	[more]
M5XRG4_PRUPE	3.3e-11	66.6	Uncharacterized protein OS=Pru...	[more]
G8E4B6_9ROSI	7.3e-11	63.3	4Fe-4S ferredoxin iron-sulfur ...	[more]

BLAST of Spo16374.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo16374.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021039	Iron-sulphur binding protein LdpA, C-terminal	PFAM	PF12617	LdpA_C	coord: 5..63 score: 5.3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0009113	purine nucleobase biosynthetic process
biological_process	GO:0001944	vasculature development
biological_process	GO:0000003	reproduction
biological_process	GO:0006189	'de novo' IMP biosynthetic process
biological_process	GO:0009116	nucleoside metabolic process
biological_process	GO:0019509	L-methionine salvage from methylthioadenosine
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0008152	metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0031124	mRNA 3'-end processing
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation
biological_process	GO:0006366	transcription by RNA polymerase II
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0006857	oligopeptide transport
biological_process	GO:0000165	MAPK cascade
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
biological_process	GO:0032259	methylation
cellular_component	GO:0005849	mRNA cleavage factor complex
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0005774	vacuolar membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004637	phosphoribosylamine-glycine ligase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0022857	transmembrane transporter activity
molecular_function	GO:0019905	syntaxin binding
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008930	methylthioadenosine nucleosidase activity
molecular_function	GO:0008782	adenosylhomocysteine nucleosidase activity
molecular_function	GO:0004601	peroxidase activity
molecular_function	GO:0035673	oligopeptide transmembrane transporter activity
molecular_function	GO:0046982	protein heterodimerization activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo17386	0.71	Barchart \| Table
Spo22894	0.71	Barchart \| Table
Spo06933	0.70	Barchart \| Table
Spo03002	0.70	Barchart \| Table
Spo17220	0.69	Barchart \| Table
Spo09684	0.69	Barchart \| Table
Spo04985	0.69	Barchart \| Table
Spo06465	0.69	Barchart \| Table
Spo14942	0.68	Barchart \| Table
Spo01841	0.68	Barchart \| Table
Spo06962	0.68	Barchart \| Table
Spo21465	0.68	Barchart \| Table
Spo01840	0.67	Barchart \| Table
Spo02280	0.67	Barchart \| Table
Spo05306	0.67	Barchart \| Table
Spo12155	0.67	Barchart \| Table
Spo16421	0.67	Barchart \| Table
Spo17391	0.67	Barchart \| Table
Spo17755	0.67	Barchart \| Table
Spo19558	0.67	Barchart \| Table
Spo23346	0.67	Barchart \| Table
Spo12107	0.66	Barchart \| Table
Spo08772	0.66	Barchart \| Table
Spo09120	0.66	Barchart \| Table
Spo15426	0.66	Barchart \| Table
Spo07586	0.66	Barchart \| Table
Spo05127	0.66	Barchart \| Table
Spo16680	0.66	Barchart \| Table
Spo18549	0.66	Barchart \| Table
Spo25851	0.66	Barchart \| Table
Spo17211	0.66	Barchart \| Table
Spo16218	0.65	Barchart \| Table
Spo26833	0.65	Barchart \| Table
Spo09214	0.65	Barchart \| Table
Spo21431	0.65	Barchart \| Table
Spo05603	0.65	Barchart \| Table