Spo06556 (gene) | SpinachBase

Overview


Name	Spo06556
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	(Isoleucyl tRNA synthetase, putative) (6.1.1.5)
Location	SpoScf_12524 : 129 .. 659 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGAGAACTGAGGTAAATAAGGTACTGGAATTTGCACGAACTGAGAAGTTAATTGGGTCGAGCTTAGAAGCAAAGGTTTATATTCACACCCCGGATACAAGCCTTTCCAACAGATTACATGAGATGTGTGAAGCAGAGATCGATGCTGATTCACTTCATCATCTCTTTATTACCTCTCAGGTATTTTCACATCTTTATAGGGTAGATATTCTTTTCTCTTGTTTTGTTGTGTAATTTAAAGTTTCAAATTTATTTACTCAGTTAATAATTGGATTATATATACAGGCCGAGATTATTTCGTGTATACAGGACATTCCTAGTGGGAAAATACCATATACCGGGGAGTATGTTGTTCAAGGAAGTGATAAAAAGGTCTGGATTGGTGTGTCAAGAGCACAAGGCTCAAAATGTGAAAGATGCTGGAATTACTCAACACAAGTTGGTTCCTTCACCGAGCATCCGACTCTTTGTCCTCGCTGCTATAACGTTGTTGGCATTCAGCCGATTCCTGAACTAGCTGCTGCCACTTGA

mRNA sequence

TTGAGAACTGAGGTAAATAAGGTACTGGAATTTGCACGAACTGAGAAGTTAATTGGGTCGAGCTTAGAAGCAAAGGTTTATATTCACACCCCGGATACAAGCCTTTCCAACAGATTACATGAGATGTGTGAAGCAGAGATCGATGCTGATTCACTTCATCATCTCTTTATTACCTCTCAGGCCGAGATTATTTCGTGTATACAGGACATTCCTAGTGGGAAAATACCATATACCGGGGAGTATGTTGTTCAAGGAAGTGATAAAAAGGTCTGGATTGGTGTGTCAAGAGCACAAGGCTCAAAATGTGAAAGATGCTGGAATTACTCAACACAAGTTGGTTCCTTCACCGAGCATCCGACTCTTTGTCCTCGCTGCTATAACGTTGTTGGCATTCAGCCGATTCCTGAACTAGCTGCTGCCACTTGA

Coding sequence (CDS)

TTGAGAACTGAGGTAAATAAGGTACTGGAATTTGCACGAACTGAGAAGTTAATTGGGTCGAGCTTAGAAGCAAAGGTTTATATTCACACCCCGGATACAAGCCTTTCCAACAGATTACATGAGATGTGTGAAGCAGAGATCGATGCTGATTCACTTCATCATCTCTTTATTACCTCTCAGGCCGAGATTATTTCGTGTATACAGGACATTCCTAGTGGGAAAATACCATATACCGGGGAGTATGTTGTTCAAGGAAGTGATAAAAAGGTCTGGATTGGTGTGTCAAGAGCACAAGGCTCAAAATGTGAAAGATGCTGGAATTACTCAACACAAGTTGGTTCCTTCACCGAGCATCCGACTCTTTGTCCTCGCTGCTATAACGTTGTTGGCATTCAGCCGATTCCTGAACTAGCTGCTGCCACTTGA

Protein sequence

LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQAEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCYNVVGIQPIPELAAAT

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo06556.1	Spo06556.1	mRNA

Homology

[Close]

BLAST of Spo06556.1 vs. NCBI nr
Match: gi|902211036|gb|KNA16599.1| (hypothetical protein SOVF_087650 [Spinacia oleracea])

HSP 1 Score: 275.4 bits (703), Expect = 5.500e-71
Identity = 130/135 (96.30%), Postives = 134/135 (99.26%), Query Frame = 1


		  
Query: 7    KVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQAEIISC 66
            +VLEFARTEKLIGSSLEAKVYIHTPD SLSNRLHEMCEAEIDADSLHHLFITSQAEIISC
Sbjct: 930  EVLEFARTEKLIGSSLEAKVYIHTPDASLSNRLHEMCEAEIDADSLHHLFITSQAEIISC 989

Query: 67   IQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCY 126
            ++DIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCY
Sbjct: 990  MEDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCY 1049

Query: 127  NVVGIQPIPELAAAT 142
            NVVG+QPIPELAAAT
Sbjct: 1050 NVVGVQPIPELAAAT 1064

[Close]

BLAST of Spo06556.1 vs. NCBI nr
Match: gi|731355411|ref|XP_010689126.1| (PREDICTED: isoleucine--tRNA ligase, mitochondrial isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 248.1 bits (632), Expect = 9.500e-63
Identity = 116/140 (82.86%), Postives = 129/140 (92.14%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNK+LE ARTEKLIGSSLEAKVYIHTPD+ L+NRLH+MCEAE DAD LH +FITSQ
Sbjct: 931  LRTEVNKILEVARTEKLIGSSLEAKVYIHTPDSRLANRLHQMCEAENDADLLHRIFITSQ 990

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
            AEIIS +++IPS KI Y+GEY++QGSD K WIGVSRA+GSKCERCWNYSTQVGSFT+HPT
Sbjct: 991  AEIISSLENIPSEKISYSGEYMIQGSDHKAWIGVSRAEGSKCERCWNYSTQVGSFTDHPT 1050

Query: 121  LCPRCYNVVGIQPIPELAAA 141
            LCPRCYNVVG QPIPELAAA
Sbjct: 1051 LCPRCYNVVGTQPIPELAAA 1070

[Close]

BLAST of Spo06556.1 vs. NCBI nr
Match: gi|731355409|ref|XP_010689125.1| (PREDICTED: isoleucine--tRNA ligase, mitochondrial isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 248.1 bits (632), Expect = 9.500e-63
Identity = 116/140 (82.86%), Postives = 129/140 (92.14%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNK+LE ARTEKLIGSSLEAKVYIHTPD+ L+NRLH+MCEAE DAD LH +FITSQ
Sbjct: 932  LRTEVNKILEVARTEKLIGSSLEAKVYIHTPDSRLANRLHQMCEAENDADLLHRIFITSQ 991

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
            AEIIS +++IPS KI Y+GEY++QGSD K WIGVSRA+GSKCERCWNYSTQVGSFT+HPT
Sbjct: 992  AEIISSLENIPSEKISYSGEYMIQGSDHKAWIGVSRAEGSKCERCWNYSTQVGSFTDHPT 1051

Query: 121  LCPRCYNVVGIQPIPELAAA 141
            LCPRCYNVVG QPIPELAAA
Sbjct: 1052 LCPRCYNVVGTQPIPELAAA 1071

[Close]

BLAST of Spo06556.1 vs. NCBI nr
Match: gi|731355413|ref|XP_010689127.1| (PREDICTED: probable isoleucine--tRNA ligase, mitochondrial isoform X3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 248.1 bits (632), Expect = 9.500e-63
Identity = 116/140 (82.86%), Postives = 129/140 (92.14%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
           LRTEVNK+LE ARTEKLIGSSLEAKVYIHTPD+ L+NRLH+MCEAE DAD LH +FITSQ
Sbjct: 839 LRTEVNKILEVARTEKLIGSSLEAKVYIHTPDSRLANRLHQMCEAENDADLLHRIFITSQ 898

Query: 61  AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
           AEIIS +++IPS KI Y+GEY++QGSD K WIGVSRA+GSKCERCWNYSTQVGSFT+HPT
Sbjct: 899 AEIISSLENIPSEKISYSGEYMIQGSDHKAWIGVSRAEGSKCERCWNYSTQVGSFTDHPT 958

Query: 121 LCPRCYNVVGIQPIPELAAA 141
           LCPRCYNVVG QPIPELAAA
Sbjct: 959 LCPRCYNVVGTQPIPELAAA 978

[Close]

BLAST of Spo06556.1 vs. NCBI nr
Match: gi|976903807|gb|KVH91571.1| (Aminoacyl-tRNA synthetase, class 1a, anticodon-binding [Cynara cardunculus var. scolymus])

HSP 1 Score: 206.5 bits (524), Expect = 3.200e-50
Identity = 95/141 (67.38%), Postives = 117/141 (82.98%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNK LE ART KLIGSSLEAKVY+H+ D  L+ RL  MCEA IDADSLH +FITSQ
Sbjct: 941  LRTEVNKALESARTGKLIGSSLEAKVYLHSSDDILAARLTRMCEANIDADSLHRIFITSQ 1000

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
             EI+S ++D P+  IPY+GEY+++G +KK+WIG+SRA+GSKCERCWN+S QVGSF +HPT
Sbjct: 1001 VEILSSLEDAPTANIPYSGEYLIEG-EKKIWIGISRAEGSKCERCWNFSPQVGSFVDHPT 1060

Query: 121  LCPRCYNVVGIQPIPELAAAT 142
            LC RCY+V+  QP+P LAA +
Sbjct: 1061 LCRRCYDVIADQPLPALAAVS 1080

[Close]

BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RC93_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_087650 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 3.900e-71
Identity = 130/135 (96.30%), Postives = 134/135 (99.26%), Query Frame = 1


		  
Query: 7    KVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQAEIISC 66
            +VLEFARTEKLIGSSLEAKVYIHTPD SLSNRLHEMCEAEIDADSLHHLFITSQAEIISC
Sbjct: 930  EVLEFARTEKLIGSSLEAKVYIHTPDASLSNRLHEMCEAEIDADSLHHLFITSQAEIISC 989

Query: 67   IQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCY 126
            ++DIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCY
Sbjct: 990  MEDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPTLCPRCY 1049

Query: 127  NVVGIQPIPELAAAT 142
            NVVG+QPIPELAAAT
Sbjct: 1050 NVVGVQPIPELAAAT 1064

[Close]

BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EH55_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g205610 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 6.600e-63
Identity = 116/140 (82.86%), Postives = 129/140 (92.14%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
           LRTEVNK+LE ARTEKLIGSSLEAKVYIHTPD+ L+NRLH+MCEAE DAD LH +FITSQ
Sbjct: 839 LRTEVNKILEVARTEKLIGSSLEAKVYIHTPDSRLANRLHQMCEAENDADLLHRIFITSQ 898

Query: 61  AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
           AEIIS +++IPS KI Y+GEY++QGSD K WIGVSRA+GSKCERCWNYSTQVGSFT+HPT
Sbjct: 899 AEIISSLENIPSEKISYSGEYMIQGSDHKAWIGVSRAEGSKCERCWNYSTQVGSFTDHPT 958

Query: 121 LCPRCYNVVGIQPIPELAAA 141
           LCPRCYNVVG QPIPELAAA
Sbjct: 959 LCPRCYNVVGTQPIPELAAA 978

[Close]

BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
Match: A0A118JU75_CYNCS (Aminoacyl-tRNA synthetase, class 1a, anticodon-binding OS=Cynara cardunculus var. scolymus GN=Ccrd_006397 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.200e-50
Identity = 95/141 (67.38%), Postives = 117/141 (82.98%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNK LE ART KLIGSSLEAKVY+H+ D  L+ RL  MCEA IDADSLH +FITSQ
Sbjct: 941  LRTEVNKALESARTGKLIGSSLEAKVYLHSSDDILAARLTRMCEANIDADSLHRIFITSQ 1000

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
             EI+S ++D P+  IPY+GEY+++G +KK+WIG+SRA+GSKCERCWN+S QVGSF +HPT
Sbjct: 1001 VEILSSLEDAPTANIPYSGEYLIEG-EKKIWIGISRAEGSKCERCWNFSPQVGSFVDHPT 1060

Query: 121  LCPRCYNVVGIQPIPELAAAT 142
            LC RCY+V+  QP+P LAA +
Sbjct: 1061 LCRRCYDVIADQPLPALAAVS 1080

[Close]

BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
Match: B9HXV6_POPTR (tRNA synthetase class I family protein OS=Populus trichocarpa GN=POPTR_0010s01830g PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 5.400e-49
Identity = 96/140 (68.57%), Postives = 115/140 (82.14%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
           LRTEVNKVLE ART KLIGSSLEAKVY+H  D SL++RLHEMC A  DAD+LH +FITSQ
Sbjct: 842 LRTEVNKVLEVARTGKLIGSSLEAKVYLHASDASLASRLHEMCTASNDADALHRIFITSQ 901

Query: 61  AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
           AE++  ++D     IPY+GEY++QGS+ +VWIGVSRA+G KCERCWNY+ QVGSF EHPT
Sbjct: 902 AEMLHHVEDEVLKAIPYSGEYLIQGSE-RVWIGVSRAEGLKCERCWNYTPQVGSFVEHPT 961

Query: 121 LCPRCYNVVGIQPIPELAAA 141
           LC RCY VV +QP P ++AA
Sbjct: 962 LCGRCYKVVAVQPEPAVSAA 980

[Close]

BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
Match: A0A061E5T1_THECC (TRNA synthetase class I (I, L, M and V) family protein isoform 1 OS=Theobroma cacao GN=TCM_009231 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.200e-48
Identity = 98/141 (69.50%), Postives = 116/141 (82.27%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNKVLE ART KLIGSSLEAKVY+HT D +L++ L EMC A  DAD+LH +F+TSQ
Sbjct: 956  LRTEVNKVLEVARTGKLIGSSLEAKVYLHTSDATLASTLLEMCSANNDADTLHRIFLTSQ 1015

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
             E+++ + +     IPYTGEY+VQ  + KVWIGVSRA+GSKCERCWNYSTQVGSF EHPT
Sbjct: 1016 VEVVASLGN-ELQNIPYTGEYLVQ--EDKVWIGVSRAEGSKCERCWNYSTQVGSFMEHPT 1075

Query: 121  LCPRCYNVVGIQPIPELAAAT 142
            LC RC++VVGIQP PE+AA T
Sbjct: 1076 LCGRCFSVVGIQPTPEMAAVT 1093

[Close]

BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
Match: SYIM_ARATH (Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=OVA2 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 3.200e-42
Identity = 85/140 (60.71%), Postives = 107/140 (76.43%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNKVLE AR EK+IGSSLEAKVY+HT D  ++ +L EM EA+ +AD+L  +FITSQ
Sbjct: 951  LRTEVNKVLELARNEKMIGSSLEAKVYLHTADAGMAAKLLEMSEAKNEADTLQRIFITSQ 1010

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
             E++S ++      + +TGEYV    + KVWIGVSRA+GSKCERCWNYS QVGSF++HPT
Sbjct: 1011 VEVLSSMEKEMISSVQHTGEYV--EGENKVWIGVSRAEGSKCERCWNYSGQVGSFSDHPT 1070

Query: 121  LCPRCYNVVGIQPIPELAAA 141
            LC RC++V+   P PE A A
Sbjct: 1071 LCGRCFSVIVANP-PEPAVA 1087

[Close]

BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
Match: SYI_MICAN (Isoleucine--tRNA ligase OS=Microcystis aeruginosa (strain NIES-843) GN=ileS PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 6.000e-25
Identity = 62/129 (48.06%), Postives = 82/129 (63.57%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLH--EMCEAEID--ADSLHHLF 60
           LRTEVNKV+E AR +K IG+SLEAKV ++ PD SL   L     C++      D L +LF
Sbjct: 824 LRTEVNKVMETARNDKAIGASLEAKVLLYVPDESLQQELELFNNCDSLTGNKVDELRYLF 883

Query: 61  ITSQAEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFT 120
           ++SQ E++S I  I       T EY  +G    V +G+ +A+G KC+RCWNYSTQVG F 
Sbjct: 884 LSSQVELVSDISAIQ------TAEY--KGESDFVSVGIVKAEGEKCDRCWNYSTQVGKFA 943

Query: 121 EHPTLCPRC 126
           + PT+C RC
Sbjct: 944 DDPTICERC 944

[Close]

BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
Match: SYI_NOSS1 (Isoleucine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / UTEX 2576) GN=ileS PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 9.600e-23
Identity = 60/126 (47.62%), Postives = 74/126 (58.73%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMC-EAEIDADSLHHLFITS 60
           LRT+VNKVLE AR EK+IGSSLEAK  I+  D +    +  +  E     D L +LF+TS
Sbjct: 835 LRTDVNKVLEQARVEKMIGSSLEAKALIYVKDANSRKAIATLNPEVGNGVDELRYLFLTS 894

Query: 61  QAEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHP 120
           Q E++     +  GK  YT +    G      IGV  A+G KC+RCWNYST VG   EHP
Sbjct: 895 QVELLDSADKLQDGK--YTSQSDSWG------IGVVNAEGQKCDRCWNYSTHVGESAEHP 952

Query: 121 TLCPRC 126
            LC RC
Sbjct: 955 LLCERC 952

[Close]

BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
Match: SYI_THEEB (Isoleucine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) GN=ileS PE=3 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 1.200e-22
Identity = 61/129 (47.29%), Postives = 76/129 (58.91%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEI----DADSLHHLF 60
           LR EVNKVLE AR EKLIGSSLEAKV+++  D+   +RL +M   +       D L +LF
Sbjct: 826 LRLEVNKVLEKARAEKLIGSSLEAKVWLYVADSEWRDRLAQMNPRDALSGNGVDELRYLF 885

Query: 61  ITSQAEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFT 120
           + SQ E++   Q +    +PY  E       + +WIGV  AQG KC RCWNYS  VG   
Sbjct: 886 LVSQVELMPQPQAVD---VPYVLEA------EGLWIGVGHAQGEKCVRCWNYSESVGQSA 945

Query: 121 EHPTLCPRC 126
            HP LC RC
Sbjct: 946 LHPQLCDRC 945

[Close]

BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
Match: SYI_CYAP8 (Isoleucine--tRNA ligase OS=Cyanothece sp. (strain PCC 8801) GN=ileS PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.400e-21
Identity = 55/129 (42.64%), Postives = 76/129 (58.91%), Query Frame = 1


		  
Query: 1   LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEI----DADSLHHLF 60
           +RTEVNKVLE AR EK IGSSL+AKV ++  D     +L      +       D L +L 
Sbjct: 831 IRTEVNKVLEQARNEKAIGSSLDAKVLLYVSDQDFKKQLESFNPNDSLKGNQVDELRYLV 890

Query: 61  ITSQAEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFT 120
           + SQ E++  ++ I   K  Y      Q   + V +GV +A+G KC+RCWNYST+VG F+
Sbjct: 891 LASQVELVDSLEAIK--KADY------QSESELVSVGVVKAEGQKCDRCWNYSTKVGEFS 950

Query: 121 EHPTLCPRC 126
           + PT+C RC
Sbjct: 951 DDPTICERC 951

[Close]

BLAST of Spo06556.1 vs. TAIR (Arabidopsis)
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein)

HSP 1 Score: 172.6 bits (436), Expect = 1.800e-43
Identity = 85/140 (60.71%), Postives = 107/140 (76.43%), Query Frame = 1


		  
Query: 1    LRTEVNKVLEFARTEKLIGSSLEAKVYIHTPDTSLSNRLHEMCEAEIDADSLHHLFITSQ 60
            LRTEVNKVLE AR EK+IGSSLEAKVY+HT D  ++ +L EM EA+ +AD+L  +FITSQ
Sbjct: 951  LRTEVNKVLELARNEKMIGSSLEAKVYLHTADAGMAAKLLEMSEAKNEADTLQRIFITSQ 1010

Query: 61   AEIISCIQDIPSGKIPYTGEYVVQGSDKKVWIGVSRAQGSKCERCWNYSTQVGSFTEHPT 120
             E++S ++      + +TGEYV    + KVWIGVSRA+GSKCERCWNYS QVGSF++HPT
Sbjct: 1011 VEVLSSMEKEMISSVQHTGEYV--EGENKVWIGVSRAEGSKCERCWNYSGQVGSFSDHPT 1070

Query: 121  LCPRCYNVVGIQPIPELAAA 141
            LC RC++V+   P PE A A
Sbjct: 1071 LCGRCFSVIVANP-PEPAVA 1087

The following BLAST results are available for this feature:

BLAST of Spo06556.1 vs. NCBI nr
BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
BLAST of Spo06556.1 vs. TAIR (Arabidopsis)

BLAST of Spo06556.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902211036\|gb\|KNA16599.1\|	5.5e-71	96.3	hypothetical protein SOVF_0876...	[more]
gi\|731355411\|ref\|XP_010689126.1\|	9.5e-63	82.8	PREDICTED: isoleucine--tRNA li...	[more]
gi\|731355409\|ref\|XP_010689125.1\|	9.5e-63	82.8	PREDICTED: isoleucine--tRNA li...	[more]
gi\|731355413\|ref\|XP_010689127.1\|	9.5e-63	82.8	PREDICTED: probable isoleucine...	[more]
gi\|976903807\|gb\|KVH91571.1\|	3.2e-50	67.3	Aminoacyl-tRNA synthetase, cla...	[more]

BLAST of Spo06556.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RC93_SPIOL	3.9e-71	96.3	Uncharacterized protein OS=Spi...	[more]
A0A0J8EH55_BETVU	6.6e-63	82.8	Uncharacterized protein OS=Bet...	[more]
A0A118JU75_CYNCS	2.2e-50	67.3	Aminoacyl-tRNA synthetase, cla...	[more]
B9HXV6_POPTR	5.4e-49	68.5	tRNA synthetase class I family...	[more]
A0A061E5T1_THECC	1.2e-48	69.5	TRNA synthetase class I (I, L,...	[more]

BLAST of Spo06556.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
SYIM_ARATH	3.2e-42	60.7	Isoleucine--tRNA ligase, chlor...	[more]
SYI_MICAN	6.0e-25	48.0	Isoleucine--tRNA ligase OS=Mic...	[more]
SYI_NOSS1	9.6e-23	47.6	Isoleucine--tRNA ligase OS=Nos...	[more]
SYI_THEEB	1.2e-22	47.2	Isoleucine--tRNA ligase OS=The...	[more]
SYI_CYAP8	2.4e-21	42.6	Isoleucine--tRNA ligase OS=Cya...	[more]

BLAST of Spo06556.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G49030.1	1.8e-43	60.7	tRNA synthetase class I (I, L,...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding	unknown	SSF47323	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	coord: 2..129 score: 1.77
IPR010663	Zinc finger, FPG/IleRS-type	PFAM	PF06827	zf-FPG_IleRS	coord: 99..127 score: 2.

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016043	cellular component organization
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0006428	isoleucyl-tRNA aminoacylation
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0009791	post-embryonic development
biological_process	GO:0006450	regulation of translational fidelity
biological_process	GO:0022414	reproductive process
biological_process	GO:0048608	reproductive structure development
biological_process	GO:0009099	valine biosynthetic process
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004822	isoleucine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo17016	0.68	Barchart \| Table