Spo25701 (gene) | SpinachBase

Overview


Name	Spo25701
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	(1-aminocyclopropane-1-carboxylate oxidase-like protein) (Gibberellin 20 oxidase)
Location	Super_scaffold_205 : 835614 .. 837869 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAATCCGCGATCCTTGGCATGAACCACGGGTCTCAGTTGTTGTGTTCTTCAACCCTGGCATGATGGAGAACTTGTATGGAACGATACCAGAGATAGTATCACCTGAAGAGCCTGCACAACCTTCAAATTATTCTAATGAGCTGTTAGAGCATGAGCTCTTTCTTGCTTCCTCGGTGCAAGATTTCAACGAAGGTATTCATTTACAAATGATTCTAGCTTGTTAAACTTGATTTCCCCATAACAATGAAGAATAGAGTGCCTTGATCTTGAAAGATCGAGGGTTATGTGCAGTATATGTGTATAGCATCTGTCACGTATGAGACACATATCTTTTTCCCTCCCCAATAGGTGTATAGTATCGATCTTGTATCTAGTCACTGCTCAGGTTAAGACTAAGACTTTAAACATCAGGATCATATTTATAAGATGAGGCCAGAGTAACAGAAATTATTCAAATAAAAAACAATTATAAACAGAGTGAAAAGTACATTTTCAGAAGGCATAAGGAAAGGAGAGAGAGAACAAGAGTAGGCTGTAGAGAGTAGGGAGTAATAGGGTGTGCAGAACCCTACATATAGTACAATAGGAATTATCAGGAGAATGTAGTTAAAAAATTGAAACAGAATTACTGATTTCTGACTAGCTAGTGATGATGTGACGTTGACGGGGTCTGATAGTGTATGGATCAAATGTATTACTGCAGTCTGTAGTCTGTAATCTGTTTATACAATACGTGTTCTTGTAGATTTTTCTCTTATGTGAATAAAATTTGATTGGTTGCCTTATTTTAGTCTTCGTTCTTGTTATGCAGAATGAATTAAATGGTTAATGCACTAAACATCGAGTTGTGAGGATGAAAATTGTTGTTCAAAAGCTGGAGTTGAAACAGGTGCAAGTGGGTATTAATTCCTGTCCGGCACCTGTTTCATCAGTAATCGACATTGGTTAAGTTTACAAATTTGGGTACTTCCTGAAAGTTCAATATTCACTCATTTTCTGAAAAACAGTGCATTGAATCCCTGGTCCAATGTGTTTATGTGGGTTATGTATGATCTCAAGTGTTCCTAAAGAATCTTAACCAGAAAACTCGCTAATGATCCTCTTTAGTTTCTATGGTACAGGCTTCTATTAGAATACGGTTGAATGAAATATCACTTGCTGATTTTTTTATACATGTTACATGTCTTTGCGAGGTTACATACAGCTTTTGTAGCACCTAGCTCTCGTGTCTTTGGAAGAGGTGAACTTGTATTAGATAGGAAGGAGATTGCTATGAGGTATATCAGATCAGGTTTCTTCATTGATCTCATTGCAGCTTTACCTTTTCCTCATATACAACTAATTCTTAGTTTTTTATTACCAAACATTAACATTTTAATGTCTACTACTTGTTATGGAAAGAAAATTGTTATGTTTTTTCGCTGCAGATTGTGATATTGTTTATAATTCCAGCAATTAAGCTGTCTAAATCAAGAATTGAAGCACCTATAATGCACTCCAATTGATTGTTTTCTTCCAATATATTCCACGGTCTTATCAGATATTCCCTTTGAGTGTTGAGATTATAAAGTCGGCTGGTGTTGTCACGAAAACAGCCTGGGCAGGAGCTGCTTATAATCTCATGTTCTACCAAGTGGATACAGATACAAGTATGTCCTATGATTTATGTTTATTGCAATTTTTATTTGTTTTTTAAAGTTTTCAAAAGTAATTGATTGTATTTTTCCTGTGTAGTGTCCTCGGCTAAAGAATGTCATGGATTCTTGATATTATGTCACGAGATACATGAAAGAGATCATCGACCATAAGCAAACTCGAATATCGGAAGATGTATGTATTTTTCTTTATTCAATTAAGCCTAGACATTAGTATATGCATTGTCACTAATTATGTTAATTTTATCATTTTGTAGTACTTTCATGATTGCAATATTCCGAATTACGATCAAGGGCAAATTGATGAAGTCATAGCTCAATGGACGCGTCATGTTCTTCTCGTGTGAATATTGTGATGTATTAATTATGTTTTTCGACTAGAATTTCTCTAGAACGTTTTAATTTTATTTTATTGGTTTGAAATTAAAAGAAGTACTATATGACAACGATACTTCTTTCTGTTTTCTAGTGTGTTTTTAGTCTACTTGTATTTAGGCATATATGATTTTAAGTTATGCACGAGACATCGAGACGTTTATTGATATGATAGAAATGACGTTGGATCTTATATATATTCAATTTGATTACTTTGTGTAG

mRNA sequence

ATGCTAATCCGCGATCCTTGGCATGAACCACGGGTCTCAGTTGTTGTGTTCTTCAACCCTGGCATGATGGAGAACTTGTATGGAACGATACCAGAGATAGTATCACCTGAAGAGCCTGCACAACCTTCAAATTATTCTAATGAGCTGTTAGAGCATGAGCTCTTTCTTGCTTCCTCGGTGCAAGATTTCAACGAAGAATGAATTAAATGGTTAATGCACTAAACATCGAGTTGTGAGGATGAAAATTGTTGTTCAAAAGCTGGAGTTGAAACAGGTGCAAGTGGGTATTAATTCCTGTCCGGCACCTGTTTCATCAGTAATCGACATTGGTTAAGTTTACAAATTTGGCTTTTGTAGCACCTAGCTCTCGTGTCTTTGGAAGAGGTGAACTTGTATTAGATAGGAAGGAGATTGCTATGAGATTGTGATATTGTTTATAATTCCAGCAATTAAGCTGTCTAAATCAAGAATTGAAGCACCTATAATGCACTCCAATTGATTGTTTTCTTCCAATATATTCCACGGTCTTATCAGATATTCCCTTTGAGTGTTGAGATTATAAAGTCGGCTGGTGTTGTCACGAAAACAGCCTGGGCAGGAGCTGCTTATAATCTCATGTTCTACCAAGTGGATACAGATACAATGTCCTCGGCTAAAGAATGTCATGGATTCTTGATATTATGTCACGAGATACATGAAAGAGATCATCGACCATAAGCAAACTCGAATATCGGAAGATTACTTTCATGATTGCAATATTCCGAATTACGATCAAGGGCAAATTGATGAAGTCATAGCTCAATGGACGCGTCATGTTCTTCTCGTGTGAATATTGTGATGTATTAATTATGTTTTTCGACTAGAATTTCTCTAGAACGTTTTAATTTTATTTTATTGGTTTGAAATTAAAAGAAGTACTATATGACAACGATACTTCTTTCTGTTTTCTAGTGTGTTTTTAGTCTACTTGTATTTAGGCATATATGATTTTAAGTTATGCACGAGACATCGAGACGTTTATTGATATGATAGAAATGACGTTGGATCTTATATATATTCAATTTGATTACTTTGTGTAG

Coding sequence (CDS)

ATGCTAATCCGCGATCCTTGGCATGAACCACGGGTCTCAGTTGTTGTGTTCTTCAACCCTGGCATGATGGAGAACTTGTATGGAACGATACCAGAGATAGTATCACCTGAAGAGCCTGCACAACCTTCAAATTATTCTAATGAGCTGTTAGAGCATGAGCTCTTTCTTGCTTCCTCGGTGCAAGATTTCAACGAAGAATGA

Protein sequence

MLIRDPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQPSNYSNELLEHELFLASSVQDFNEE

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo25701.1	Spo25701.1	mRNA

Homology

[Close]

BLAST of Spo25701.1 vs. NCBI nr
Match: gi|902209559|gb|KNA16231.1| (hypothetical protein SOVF_090750 [Spinacia oleracea])

HSP 1 Score: 117.5 bits (293), Expect = 9.000e-24
Identity = 55/61 (90.16%), Postives = 57/61 (93.44%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQPSNYSNELLEHELFLASSVQDFN 64
           +PWHEPRVSVVVFFNP MMENLYG IPEIVSPEEPAQPSNYSNE LE ELFLASSVQDFN
Sbjct: 91  NPWHEPRVSVVVFFNPSMMENLYGPIPEIVSPEEPAQPSNYSNEQLERELFLASSVQDFN 150

Query: 65  E 66
           +
Sbjct: 151 Q 151

[Close]

BLAST of Spo25701.1 vs. NCBI nr
Match: gi|902237995|gb|KNA25000.1| (hypothetical protein SOVF_010500 [Spinacia oleracea])

HSP 1 Score: 63.2 bits (152), Expect = 2.000e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVS+ VFFNP M +NLYG IPE VSPEEPA
Sbjct: 301 NPQHEPRVSIAVFFNPSMRDNLYGPIPETVSPEEPA 336

[Close]

BLAST of Spo25701.1 vs. NCBI nr
Match: gi|731361633|ref|XP_010692464.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 4-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.8 bits (151), Expect = 2.600e-7
Identity = 34/67 (50.75%), Postives = 42/67 (62.69%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQP-----SNYSNELLEHELFLASS 64
           +P HEPRVS+ VFFNPG+ ENLYG +PEIVS EEPA       +N+     + EL   S 
Sbjct: 276 NPLHEPRVSIAVFFNPGVRENLYGPLPEIVSAEEPAHYQQFTFTNFITRFFKKELDGKSL 335

Query: 65  VQDFNEE 67
           V  F+ E
Sbjct: 336 VNYFSLE 342

[Close]

BLAST of Spo25701.1 vs. NCBI nr
Match: gi|870847771|gb|KMT00119.1| (hypothetical protein BVRB_1g019420 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.8 bits (151), Expect = 2.600e-7
Identity = 34/67 (50.75%), Postives = 42/67 (62.69%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQP-----SNYSNELLEHELFLASS 64
           +P HEPRVS+ VFFNPG+ ENLYG +PEIVS EEPA       +N+     + EL   S 
Sbjct: 301 NPLHEPRVSIAVFFNPGVRENLYGPLPEIVSAEEPAHYQQFTFTNFITRFFKKELDGKSL 360

Query: 65  VQDFNEE 67
           V  F+ E
Sbjct: 361 VNYFSLE 367

[Close]

BLAST of Spo25701.1 vs. NCBI nr
Match: gi|731361299|ref|XP_010692279.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 4-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.4 bits (150), Expect = 3.400e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVSV VFFNP M ENLYG +PE+VS EEPA
Sbjct: 296 NPLHEPRVSVAVFFNPSMRENLYGPLPEVVSAEEPA 331

[Close]

BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R9P7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_090750 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 6.300e-24
Identity = 55/61 (90.16%), Postives = 57/61 (93.44%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQPSNYSNELLEHELFLASSVQDFN 64
           +PWHEPRVSVVVFFNP MMENLYG IPEIVSPEEPAQPSNYSNE LE ELFLASSVQDFN
Sbjct: 91  NPWHEPRVSVVVFFNPSMMENLYGPIPEIVSPEEPAQPSNYSNEQLERELFLASSVQDFN 150

Query: 65  E 66
           +
Sbjct: 151 Q 151

[Close]

BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RZS0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_010500 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.400e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVS+ VFFNP M +NLYG IPE VSPEEPA
Sbjct: 301 NPQHEPRVSIAVFFNPSMRDNLYGPIPETVSPEEPA 336

[Close]

BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BJ88_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g019420 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.800e-7
Identity = 34/67 (50.75%), Postives = 42/67 (62.69%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQP-----SNYSNELLEHELFLASS 64
           +P HEPRVS+ VFFNPG+ ENLYG +PEIVS EEPA       +N+     + EL   S 
Sbjct: 301 NPLHEPRVSIAVFFNPGVRENLYGPLPEIVSAEEPAHYQQFTFTNFITRFFKKELDGKSL 360

Query: 65  VQDFNEE 67
           V  F+ E
Sbjct: 361 VNYFSLE 367

[Close]

BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BJ89_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g019410 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.400e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1

		  
Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVSV VFFNP M ENLYG +PE+VS EEPA
Sbjct: 296 NPLHEPRVSVAVFFNPSMRENLYGPLPEVVSAEEPA 331

[Close]

BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HU63_SOLCH (Putative 1-aminocyclopropane-1-carboxylate oxidase-like OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.500e-6
Identity = 22/39 (56.41%), Postives = 32/39 (82.05%), Query Frame = 1

		  
Query: 2   LIRDPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           ++ +P+HEPRVSV +FFNPG  EN +G +PE++S E+PA
Sbjct: 172 VLANPFHEPRVSVAIFFNPGQRENSFGPLPELISDEKPA 210

The following BLAST results are available for this feature:

BLAST of Spo25701.1 vs. NCBI nr
BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
BLAST of Spo25701.1 vs. ExPASy Swiss-Prot
BLAST of Spo25701.1 vs. TAIR (Arabidopsis)

BLAST of Spo25701.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
gi\|902209559\|gb\|KNA16231.1\|	9.0e-24	90.1	hypothetical protein SOVF_0907...	[more]
gi\|902237995\|gb\|KNA25000.1\|	2.0e-7	75.0	hypothetical protein SOVF_0105...	[more]
gi\|731361633\|ref\|XP_010692464.1\|	2.6e-7	50.7	PREDICTED: 1-aminocyclopropane...	[more]
gi\|870847771\|gb\|KMT00119.1\|	2.6e-7	50.7	hypothetical protein BVRB_1g01...	[more]
gi\|731361299\|ref\|XP_010692279.1\|	3.4e-7	75.0	PREDICTED: 1-aminocyclopropane...	[more]

BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
A0A0K9R9P7_SPIOL	6.3e-24	90.1	Uncharacterized protein OS=Spi...	[more]
A0A0K9RZS0_SPIOL	1.4e-7	75.0	Uncharacterized protein OS=Spi...	[more]
A0A0J8BJ88_BETVU	1.8e-7	50.7	Uncharacterized protein OS=Bet...	[more]
A0A0J8BJ89_BETVU	2.4e-7	75.0	Uncharacterized protein OS=Bet...	[more]
A0A0V0HU63_SOLCH	4.5e-6	56.4	Putative 1-aminocyclopropane-1...	[more]

BLAST of Spo25701.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description

BLAST of Spo25701.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001101	response to acid chemical
biological_process	GO:0071806	protein transmembrane transport
biological_process	GO:0006950	response to stress
biological_process	GO:0006189	'de novo' IMP biosynthetic process
biological_process	GO:0006810	transport
biological_process	GO:0048868	pollen tube development
biological_process	GO:0009628	response to abiotic stimulus
biological_process	GO:0034655	nucleobase-containing compound catabolic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006260	DNA replication
biological_process	GO:0006281	DNA repair
biological_process	GO:0044265	cellular macromolecule catabolic process
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0016192	vesicle-mediated transport
biological_process	GO:0009113	purine nucleobase biosynthetic process
biological_process	GO:1901700	response to oxygen-containing compound
cellular_component	GO:0005744	mitochondrial inner membrane presequence translocase complex
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009527	plastid outer membrane
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0031981	nuclear lumen
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0015450	P-P-bond-hydrolysis-driven protein transmembrane transporter activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004527	exonuclease activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0004637	phosphoribosylamine-glycine ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0005515	protein binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo14240	0.69	Barchart \| Table
Spo14942	0.69	Barchart \| Table
Spo02749	0.69	Barchart \| Table
Spo24369	0.67	Barchart \| Table
Spo22436	0.67	Barchart \| Table
Spo07685	0.67	Barchart \| Table
Spo00716	0.67	Barchart \| Table
Spo24736	0.67	Barchart \| Table
Spo24886	0.66	Barchart \| Table
Spo09214	0.66	Barchart \| Table
Spo11285	0.66	Barchart \| Table
Spo16186	0.66	Barchart \| Table
Spo24018	0.65	Barchart \| Table
Spo22694	0.65	Barchart \| Table