Spo20357 (gene) | SpinachBase

Overview


Name	Spo20357
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Heavy metal transport/detoxification superfamily protein
Location	Super_scaffold_7 : 3524187 .. 3525926 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTACACTGAGGACAACCTTATGTCCTGTCCTTACTATACTACAAGTTCTTCATTAACTTTATCATATAGTTTTGTCATTCATTAACCAACTTATTCCACTCCTTAATAATATTATTTTCACAATTAAAATTATCGTAAAGTCCTCCTCGAGGTAGGACCATCAGTTAATTTATGATGTGCAAGAATTTAGCCAAGGTTACTACCAAATCTACCACTTCTAACTACAACTTCTTGTCTTATCAGTTGTCACTCTTTCTTTCTCCTCTTCCCTCAATAATTCTTCCCACTACCTTAGTTTATATATACCCTTTTCGTCACCAATAAGTTATTGTCTCATCAAAATAAATCATATAAATTAAATCAATTAGAGTAAAGACTATTTATAGAAGGGCATAAAGTGTTTTGTTACTATAAATAGGACAAAGTAATTAAGCAAGAGTAGTGGAATTAGAACGATACAAACAAGACTAGAATAACTCTTGAGCAGAAGAATTAAGAGACATAATGGGAGTTCAAGGAATTTTGGAGTATTTCTCAGATTTGATAAGGAGTGCTAAGAAAGGAAAGAAAAGGAAGCAATTAAACACGATTAATCTCAAGATTACAAGAATTGATTGTGAGGGTTGTGCTATGAAAATCAAGAAAGCGCTCTCTGGACTCAAAGGTACTTTATTTTCTTCTTATTGAAATTAATTATCCATTAACAACATGTATGTAAACTTTCCCCTAAAAAAAGCATGCGTGTAAACTTAATTTTATAAGTTTACTTGTCATATCTTGAGCTAATTTGTGCATTTTATTTTGATTTCCAAAATTTTATGTGTTGTTTGAAAAAAGAAACACTTAATGTTCATTATTTCATGCAACTTAATTAACTAGACTATCATAAAATTTTCTTTTTTTAAATAATCACTTCTCAAAAATGATTATGTTGTCATCATATCCTAGAAAGAACAAGTATATAGGCGCTTTGTTATTTAGCAAGTAGTACTTGGTGTAGTTAGTAGGGTTTCTTATGGTCCCACCAGCTATGTTCTTGGGGTACGCGGAAATTCTTTATCCACCCCTCCAAATCCCAAAAGTATTATAACTTGTGTAGCTGAGCTAGTTTAACCGTGAGATGTTAGGTCTATAAGGGTTACTTTTCTTAACACAAAAATAGGATACTCCAAGCTAACAAGAATTAAGTTGCTGCAAATAGCTTTAAAACTAATTATTTGGGGTTATTAATAAATAAGTAAAAATGATGTGGAAGCAGGGGTGAAATCAGTGAATGTGGATATGAAACAACAGAAGGTGACAGTGACAGGATTTGTGGAAGCAAAGAAAGTGTTGGAAGCAGCAAAATCAACAAAGAAGAAGGTTGAGTTATGGCCATATGTCCCTTACACTATGGTTGCACACCCTTATATTGCTGGTGCTTATGACAAAAAAGCCCCTCCTAATTTTGTAAGGAAAGTTGATGATTCTATGACTCCTTTGGAACAACAATATACACACATGTTTAGTGATGAAAATCCTGATGCTTGCTCTATTATGTAATCTTTTTTTTTTTTAATATCACATTTGTACGTAGTTTATGCAATGTGCATGTTTGTTTTGTATAAATTTGTGCTATTGTATTTGTAGATACGTGTAAGAGAGTAGGGAATATCAAATATGTTGGCTCGCTTCTAATTTTAAGGCATCCGATTTTTGTTCTGATTTTTTTGGTTATTCCCCTTGAACGGATAATATGT

mRNA sequence

TTTACACTGAGGACAACCTTATGTCCTGTCCTTACTATACTACAAGTTCTTCATTAACTTTATCATATAGTTTTGTCATTCATTAACCAACTTATTCCACTCCTTAATAATATTATTTTCACAATTAAAATTATCGTAAAGTCCTCCTCGAGGTAGGACCATCAGTTAATTTATGATGTGCAAGAATTTAGCCAAGGTTACTACCAAATCTACCACTTCTAACTACAACTTCTTGTCTTATCAGTTGTCACTCTTTCTTTCTCCTCTTCCCTCAATAATTCTTCCCACTACCTTAGTTTATATATACCCTTTTCGTCACCAATAAGTTATTGTCTCATCAAAATAAATCATATAAATTAAATCAATTAGAGTAAAGACTATTTATAGAAGGGCATAAAGTGTTTTGTTACTATAAATAGGACAAAGTAATTAAGCAAGAGTAGTGGAATTAGAACGATACAAACAAGACTAGAATAACTCTTGAGCAGAAGAATTAAGAGACATAATGGGAGTTCAAGGAATTTTGGAGTATTTCTCAGATTTGATAAGGAGTGCTAAGAAAGGAAAGAAAAGGAAGCAATTAAACACGATTAATCTCAAGATTACAAGAATTGATTGTGAGGGTTGTGCTATGAAAATCAAGAAAGCGCTCTCTGGACTCAAAGGGGTGAAATCAGTGAATGTGGATATGAAACAACAGAAGGTGACAGTGACAGGATTTGTGGAAGCAAAGAAAGTGTTGGAAGCAGCAAAATCAACAAAGAAGAAGGTTGAGTTATGGCCATATGTCCCTTACACTATGGTTGCACACCCTTATATTGCTGGTGCTTATGACAAAAAAGCCCCTCCTAATTTTGTAAGGAAAGTTGATGATTCTATGACTCCTTTGGAACAACAATATACACACATGTTTAGTGATGAAAATCCTGATGCTTGCTCTATTATGTAATCTTTTTTTTTTTTAATATCACATTTGTACGTAGTTTATGCAATGTGCATGTTTGTTTTGTATAAATTTGTGCTATTGTATTTGTAGATACGTGTAAGAGAGTAGGGAATATCAAATATGTTGGCTCGCTTCTAATTTTAAGGCATCCGATTTTTGTTCTGATTTTTTTGGTTATTCCCCTTGAACGGATAATATGT

Coding sequence (CDS)

ATGGGAGTTCAAGGAATTTTGGAGTATTTCTCAGATTTGATAAGGAGTGCTAAGAAAGGAAAGAAAAGGAAGCAATTAAACACGATTAATCTCAAGATTACAAGAATTGATTGTGAGGGTTGTGCTATGAAAATCAAGAAAGCGCTCTCTGGACTCAAAGGGGTGAAATCAGTGAATGTGGATATGAAACAACAGAAGGTGACAGTGACAGGATTTGTGGAAGCAAAGAAAGTGTTGGAAGCAGCAAAATCAACAAAGAAGAAGGTTGAGTTATGGCCATATGTCCCTTACACTATGGTTGCACACCCTTATATTGCTGGTGCTTATGACAAAAAAGCCCCTCCTAATTTTGTAAGGAAAGTTGATGATTCTATGACTCCTTTGGAACAACAATATACACACATGTTTAGTGATGAAAATCCTGATGCTTGCTCTATTATGTAA

Protein sequence

MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKVDDSMTPLEQQYTHMFSDENPDACSIM

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo20357.1	Spo20357.1	mRNA

Homology

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BLAST of Spo20357.1 vs. NCBI nr
Match: gi|731361867|ref|XP_010692589.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 254.6 bits (649), Expect = 1.100e-64
Identity = 124/147 (84.35%), Postives = 136/147 (92.52%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQG LEY SD+I SAKKGKK+KQ NT+NLK+TRIDCEGCAMKIKKALSGLKGVK+V+V
Sbjct: 1   MGVQGTLEYLSDMISSAKKGKKKKQFNTVNLKVTRIDCEGCAMKIKKALSGLKGVKTVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           DMKQQKVTVTG++EAKKVL AAKSTKKKVELWPYVPYTMVAHPY+AGAYDKKAP N VRK
Sbjct: 61  DMKQQKVTVTGYMEAKKVLAAAKSTKKKVELWPYVPYTMVAHPYMAGAYDKKAPANCVRK 120

Query: 121 VDDSMTPLEQQYTHMFSDENPDACSIM 148
           V+D MTP+E+QYT MFSDENP AC IM
Sbjct: 121 VEDPMTPMEEQYTTMFSDENPGACYIM 147

[Close]

BLAST of Spo20357.1 vs. NCBI nr
Match: gi|902165356|gb|KNA07098.1| (hypothetical protein SOVF_175040, partial [Spinacia oleracea])

HSP 1 Score: 232.6 bits (592), Expect = 4.300e-58
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV
Sbjct: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNF 118
           DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNF
Sbjct: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNF 117

[Close]

BLAST of Spo20357.1 vs. NCBI nr
Match: gi|502174981|ref|XP_004515645.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Cicer arietinum])

HSP 1 Score: 213.8 bits (543), Expect = 2.100e-52
Identity = 109/151 (72.19%), Postives = 124/151 (82.12%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQG LEY SDL+ S KK KKRKQ  T+ LKI R+DCEGCA K+K  LSG+KG KSV+V
Sbjct: 1   MGVQGTLEYLSDLLSSKKKKKKRKQTQTVALKI-RMDCEGCARKVKHVLSGVKGAKSVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           DMKQQKVTV+G+VEAKKVL+AA+STKKKVELWPYVPYTMVAHPYI+ AYDKKAPPN VRK
Sbjct: 61  DMKQQKVTVSGYVEAKKVLKAAQSTKKKVELWPYVPYTMVAHPYISQAYDKKAPPNMVRK 120

Query: 121 VDDSM----TPLEQQYTHMFSDENPDACSIM 148
           V D+     T  +  Y  MFSDENP+ACS+M
Sbjct: 121 VADTSNIKETTFDDTYVEMFSDENPNACSVM 150

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BLAST of Spo20357.1 vs. NCBI nr
Match: gi|593268065|ref|XP_007136210.1| (hypothetical protein PHAVU_009G027600g [Phaseolus vulgaris])

HSP 1 Score: 213.4 bits (542), Expect = 2.700e-52
Identity = 107/151 (70.86%), Postives = 126/151 (83.44%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQG LEYFSDL+ S KK KK+KQ+ T+ LKI R+DCEGCA K+K  LSG+KG KSV V
Sbjct: 1   MGVQGTLEYFSDLLSSTKKKKKKKQIQTVALKI-RMDCEGCARKVKHVLSGVKGAKSVEV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           D+KQQK TVTG+VEAKKVL+AA+STKKKVELWPY+PYTMVA+PY++ AYDKKAPPN VRK
Sbjct: 61  DLKQQKATVTGYVEAKKVLKAAQSTKKKVELWPYIPYTMVANPYVSQAYDKKAPPNMVRK 120

Query: 121 VDD----SMTPLEQQYTHMFSDENPDACSIM 148
           V D    S T ++ +Y  MFSDENP+ACSIM
Sbjct: 121 VADTANMSETTVDDRYIQMFSDENPNACSIM 150

[Close]

BLAST of Spo20357.1 vs. NCBI nr
Match: gi|729296598|ref|XP_010544234.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Tarenaya hassleriana])

HSP 1 Score: 210.3 bits (534), Expect = 2.300e-51
Identity = 105/151 (69.54%), Postives = 126/151 (83.44%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGV+G +EY S+L++  KK KK+KQ+ T+ L++ RIDCEGC  KIK  LSG+KGVK+V+V
Sbjct: 1   MGVEGTMEYISELLKKTKK-KKKKQMQTVALRVARIDCEGCERKIKHILSGVKGVKTVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           D KQQKVTVTG+VE KKVLEAAKSTKKKVELWPYVPYTMVAHPYI+ AYDKKAPPN VRK
Sbjct: 61  DAKQQKVTVTGYVEPKKVLEAAKSTKKKVELWPYVPYTMVAHPYISQAYDKKAPPNMVRK 120

Query: 121 VDD----SMTPLEQQYTHMFSDENPDACSIM 148
           V D    S T ++ +YT MFSDENP++CSI+
Sbjct: 121 VPDTAALSETTVDDRYTIMFSDENPNSCSIV 150

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BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BEB9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g021330 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 7.300e-65
Identity = 124/147 (84.35%), Postives = 136/147 (92.52%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQG LEY SD+I SAKKGKK+KQ NT+NLK+TRIDCEGCAMKIKKALSGLKGVK+V+V
Sbjct: 1   MGVQGTLEYLSDMISSAKKGKKKKQFNTVNLKVTRIDCEGCAMKIKKALSGLKGVKTVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           DMKQQKVTVTG++EAKKVL AAKSTKKKVELWPYVPYTMVAHPY+AGAYDKKAP N VRK
Sbjct: 61  DMKQQKVTVTGYMEAKKVLAAAKSTKKKVELWPYVPYTMVAHPYMAGAYDKKAPANCVRK 120

Query: 121 VDDSMTPLEQQYTHMFSDENPDACSIM 148
           V+D MTP+E+QYT MFSDENP AC IM
Sbjct: 121 VEDPMTPMEEQYTTMFSDENPGACYIM 147

[Close]

BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QK80_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_175040 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.000e-58
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV
Sbjct: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNF 118
           DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNF
Sbjct: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNF 117

[Close]

BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
Match: V7ARL8_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G027600g PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.900e-52
Identity = 107/151 (70.86%), Postives = 126/151 (83.44%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGVQG LEYFSDL+ S KK KK+KQ+ T+ LKI R+DCEGCA K+K  LSG+KG KSV V
Sbjct: 1   MGVQGTLEYFSDLLSSTKKKKKKKQIQTVALKI-RMDCEGCARKVKHVLSGVKGAKSVEV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           D+KQQK TVTG+VEAKKVL+AA+STKKKVELWPY+PYTMVA+PY++ AYDKKAPPN VRK
Sbjct: 61  DLKQQKATVTGYVEAKKVLKAAQSTKKKVELWPYIPYTMVANPYVSQAYDKKAPPNMVRK 120

Query: 121 VDD----SMTPLEQQYTHMFSDENPDACSIM 148
           V D    S T ++ +Y  MFSDENP+ACSIM
Sbjct: 121 VADTANMSETTVDDRYIQMFSDENPNACSIM 150

[Close]

BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
Match: A0A0B0MKC0_GOSAR (Superoxide dismutase 1 copper chaperone OS=Gossypium arboreum GN=F383_17649 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.600e-51
Identity = 105/151 (69.54%), Postives = 127/151 (84.11%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGV+G LEY SDL+ S KK KK+KQ+ T+ LKI R+DCEGCA K+KK LSG+KG KSV+V
Sbjct: 1   MGVEGTLEYISDLLSSVKKKKKKKQIQTVALKI-RMDCEGCARKVKKVLSGVKGAKSVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           D+KQQK TVTG+VEAKKVL AA+STKKKVELWPYVPYT+VA+PY+A AYDKKAPPN VRK
Sbjct: 61  DLKQQKATVTGYVEAKKVLAAAQSTKKKVELWPYVPYTLVANPYVAQAYDKKAPPNHVRK 120

Query: 121 VDDSM----TPLEQQYTHMFSDENPDACSIM 148
           V  S     T ++ +YT++FSD+NP+ACSIM
Sbjct: 121 VPASATVSETTIDNRYTNLFSDDNPNACSIM 150

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BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
Match: A0A0D2QGZ4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G219600 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 3.500e-51
Identity = 104/151 (68.87%), Postives = 127/151 (84.11%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGV+G LEY SDL+ S KK KK+KQ+ T+ LKI R+DCEGCA K+KK LSG+KG KSV+V
Sbjct: 1   MGVEGTLEYISDLLSSVKKKKKKKQIQTVALKI-RMDCEGCARKVKKVLSGVKGAKSVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           D+KQQK TVTG+VEAKKVL AA+STKKKVELWPYVPYT+VA+PY+A AYDKKAPPN VRK
Sbjct: 61  DLKQQKATVTGYVEAKKVLAAAQSTKKKVELWPYVPYTLVANPYVAQAYDKKAPPNHVRK 120

Query: 121 VDDSM----TPLEQQYTHMFSDENPDACSIM 148
           V  +     T ++ +YT++FSD+NP+ACSIM
Sbjct: 121 VPATATVTETTIDDRYTNLFSDDNPNACSIM 150

[Close]

BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
Match: HIP22_ARATH (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana GN=HIPP22 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 8.400e-38
Identity = 81/145 (55.86%), Postives = 107/145 (73.79%), Query Frame = 1

		  
Query: 8   EYFSD-LIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDMKQQK 67
           EYFS+    S +K KKRK + T+N+K+ +IDC+GC  KIK A+S +KG KSV V+ K  K
Sbjct: 9   EYFSNHFYVSIRKRKKRKVMQTVNIKV-KIDCDGCERKIKNAVSSIKGAKSVEVNRKMHK 68

Query: 68  VTVTGFVEAKKVLEAAKST-KKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKVDDSM 127
           VTV+G+V+ KKVL+  +ST KKK ELWPYVPYTMVA+PY AGAYDK+APP FVRK + + 
Sbjct: 69  VTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVRKSEQAQ 128

Query: 128 T---PLEQQYTHMFSDENPDACSIM 148
                 + +   +FSDENP+AC++M
Sbjct: 129 AQPGSTDDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
Match: HIP20_ARATH (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN=HIPP20 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 8.400e-38
Identity = 76/150 (50.67%), Postives = 104/150 (69.33%), Query Frame = 1

		  
Query: 3   VQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDM 62
           +  + EY SD  R  +K +KRK + T+N+K+ ++DC+GC  ++K A+S +KGVKSV V+ 
Sbjct: 4   LDSLSEYISDYFRVTRKRRKRKVMQTVNIKV-KMDCDGCERRVKNAVSSMKGVKSVEVNR 63

Query: 63  KQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKVD 122
           K  KVTV+G+VE KKVL+  + T KK E+WPYVPY MVA+PY  G YDKKAP  +VRK +
Sbjct: 64  KIHKVTVSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSE 123

Query: 123 DSMTPL-----EQQYTHMFSDENPDACSIM 148
            S   L     E  Y  +FSDENP+AC++M
Sbjct: 124 QSQLQLLPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
Match: HIP26_ARATH (Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana GN=HIPP26 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 9.600e-34
Identity = 75/153 (49.02%), Postives = 104/153 (67.97%), Query Frame = 1

		  
Query: 5   GILEYFSDLIRSAK--KGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDM 64
           G+L++ S++   +   K KKRKQL T+ +K+ ++DCEGC  K+++++ G+KGV SV ++ 
Sbjct: 2   GVLDHVSEMFDCSHGHKIKKRKQLQTVEIKV-KMDCEGCERKVRRSVEGMKGVSSVTLEP 61

Query: 65  KQQKVTVTGFVEAKKVL-EAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKV 124
           K  KVTV G+V+  KV+   +  T KKVELWPYVPY +VAHPY AG YDKKAP  +VR+V
Sbjct: 62  KAHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRRV 121

Query: 125 DD-------SMTPLEQQYTHMFSDENPDACSIM 148
           DD         +  E +YT  FSDENP AC +M
Sbjct: 122 DDPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

[Close]

BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
Match: HIP27_ARATH (Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN=HIPP27 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.700e-30
Identity = 70/139 (50.36%), Postives = 91/139 (65.47%), Query Frame = 1

		  
Query: 18  KKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDMKQQKVTVTGFVEAKK 77
           K  KK KQ   + +K+ ++DCEGC  +++K++ G+KGV  V VD KQ K+TV GFV+  K
Sbjct: 10  KHHKKLKQFQKVEIKV-KMDCEGCERRVRKSVEGMKGVSKVTVDPKQSKLTVEGFVQPSK 69

Query: 78  VLEAAKS-TKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK--VDDSMTPL------ 137
           V+      T KK ELWPYVPY +V HPY  GAYDKKAPP +VR    D  + PL      
Sbjct: 70  VVHRVMHRTGKKAELWPYVPYEVVPHPYAPGAYDKKAPPGYVRNALADPLVAPLARASSF 129

Query: 138 EQQYTHMFSDENPDACSIM 148
           E +YT  FSD+NP+AC+IM
Sbjct: 130 EVKYTSAFSDDNPNACTIM 147

[Close]

BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
Match: HIP25_ARATH (Heavy metal-associated isoprenylated plant protein 25 OS=Arabidopsis thaliana GN=HIPP25 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.300e-25
Identity = 61/153 (39.87%), Postives = 93/153 (60.78%), Query Frame = 1

		  
Query: 5   GILEYFSDLIRSAKKGKKR-KQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDMK 64
           G+L++ S+    +    KR K L T+++++  IDCEGC  K+++AL G++G++ V ++  
Sbjct: 2   GVLDHVSEYFDCSHGSSKRHKSLQTVDVRVL-IDCEGCERKVRRALEGMRGIRDVTIEPN 61

Query: 65  QQKVTVTGFVEAKKVL-EAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKVD 124
            QKVTV G+VE  KV+      T K+ EL+P+VPY +VAHPY +G YD +AP  +VR  +
Sbjct: 62  AQKVTVVGYVEPNKVVARIIHRTGKRAELYPFVPYDVVAHPYASGVYDNRAPTGYVRNTE 121

Query: 125 --------DSMTPLEQQYTHMFSDENPDACSIM 148
                      +  E +YT  FSDEN  AC +M
Sbjct: 122 YDPHVSRLARASSTEVRYTTAFSDENASACVVM 153

[Close]

BLAST of Spo20357.1 vs. TAIR (Arabidopsis)
Match: AT4G08570.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 3.400e-53
Identity = 101/151 (66.89%), Postives = 125/151 (82.78%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNV 60
           MGV+G +EY SDL++  K+ KK+KQ+ T+ L++ RIDCEGC  KIK  LSG+KGVKSV+V
Sbjct: 1   MGVEGTMEYISDLLKKRKR-KKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDV 60

Query: 61  DMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRK 120
           D+K QKVTVTG+++ KKVLEAAKSTKKKVELWPYVPYTMVA+PYI+ AYDKKAPPN VRK
Sbjct: 61  DVKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRK 120

Query: 121 VDDSM----TPLEQQYTHMFSDENPDACSIM 148
           V D+     T ++  YT MFSDENP++C+IM
Sbjct: 121 VPDTASVNETTVDDSYTIMFSDENPNSCAIM 150

[Close]

BLAST of Spo20357.1 vs. TAIR (Arabidopsis)
Match: AT4G39700.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 2.200e-44
Identity = 91/159 (57.23%), Postives = 116/159 (72.96%), Query Frame = 1

		  
Query: 1   MGVQGILEYFSDLI-----RSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGV 60
           MGV G LEY S+LI      S  K KK+KQ  T+ LK+ R+DC+GC +KIK +LS LKGV
Sbjct: 1   MGVGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKV-RMDCDGCVLKIKNSLSSLKGV 60

Query: 61  KSVNVDMKQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPP 120
           K+V ++ KQQKVTV+G+ +A KVL+ AK+T KK E+WPYVPY +VA PYIA AYDKKAPP
Sbjct: 61  KTVEINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPP 120

Query: 121 NFVRKVDDSMTP-------LEQQYTHMFSDENPDACSIM 148
            +VRKVD ++T         +  YT +FSD+NP+ACSIM
Sbjct: 121 GYVRKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of Spo20357.1 vs. TAIR (Arabidopsis)
Match: AT1G71050.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 157.9 bits (398), Expect = 4.700e-39
Identity = 76/150 (50.67%), Postives = 104/150 (69.33%), Query Frame = 1

		  
Query: 3   VQGILEYFSDLIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDM 62
           +  + EY SD  R  +K +KRK + T+N+K+ ++DC+GC  ++K A+S +KGVKSV V+ 
Sbjct: 4   LDSLSEYISDYFRVTRKRRKRKVMQTVNIKV-KMDCDGCERRVKNAVSSMKGVKSVEVNR 63

Query: 63  KQQKVTVTGFVEAKKVLEAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKVD 122
           K  KVTV+G+VE KKVL+  + T KK E+WPYVPY MVA+PY  G YDKKAP  +VRK +
Sbjct: 64  KIHKVTVSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSE 123

Query: 123 DSMTPL-----EQQYTHMFSDENPDACSIM 148
            S   L     E  Y  +FSDENP+AC++M
Sbjct: 124 QSQLQLLPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Spo20357.1 vs. TAIR (Arabidopsis)
Match: AT1G22990.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 157.9 bits (398), Expect = 4.700e-39
Identity = 81/145 (55.86%), Postives = 107/145 (73.79%), Query Frame = 1

		  
Query: 8   EYFSD-LIRSAKKGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDMKQQK 67
           EYFS+    S +K KKRK + T+N+K+ +IDC+GC  KIK A+S +KG KSV V+ K  K
Sbjct: 9   EYFSNHFYVSIRKRKKRKVMQTVNIKV-KIDCDGCERKIKNAVSSIKGAKSVEVNRKMHK 68

Query: 68  VTVTGFVEAKKVLEAAKST-KKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKVDDSM 127
           VTV+G+V+ KKVL+  +ST KKK ELWPYVPYTMVA+PY AGAYDK+APP FVRK + + 
Sbjct: 69  VTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVRKSEQAQ 128

Query: 128 T---PLEQQYTHMFSDENPDACSIM 148
                 + +   +FSDENP+AC++M
Sbjct: 129 AQPGSTDDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Spo20357.1 vs. TAIR (Arabidopsis)
Match: AT4G38580.1 (farnesylated protein 6)

HSP 1 Score: 144.4 bits (363), Expect = 5.400e-35
Identity = 75/153 (49.02%), Postives = 104/153 (67.97%), Query Frame = 1

		  
Query: 5   GILEYFSDLIRSAK--KGKKRKQLNTINLKITRIDCEGCAMKIKKALSGLKGVKSVNVDM 64
           G+L++ S++   +   K KKRKQL T+ +K+ ++DCEGC  K+++++ G+KGV SV ++ 
Sbjct: 2   GVLDHVSEMFDCSHGHKIKKRKQLQTVEIKV-KMDCEGCERKVRRSVEGMKGVSSVTLEP 61

Query: 65  KQQKVTVTGFVEAKKVL-EAAKSTKKKVELWPYVPYTMVAHPYIAGAYDKKAPPNFVRKV 124
           K  KVTV G+V+  KV+   +  T KKVELWPYVPY +VAHPY AG YDKKAP  +VR+V
Sbjct: 62  KAHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRRV 121

Query: 125 DD-------SMTPLEQQYTHMFSDENPDACSIM 148
           DD         +  E +YT  FSDENP AC +M
Sbjct: 122 DDPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

The following BLAST results are available for this feature:

BLAST of Spo20357.1 vs. NCBI nr
BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
BLAST of Spo20357.1 vs. TAIR (Arabidopsis)

BLAST of Spo20357.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|731361867\|ref\|XP_010692589.1\|	1.1e-64	84.3	PREDICTED: heavy metal-associa...	[more]
gi\|902165356\|gb\|KNA07098.1\|	4.3e-58	100.	hypothetical protein SOVF_1750...	[more]
gi\|502174981\|ref\|XP_004515645.1\|	2.1e-52	72.1	PREDICTED: heavy metal-associa...	[more]
gi\|593268065\|ref\|XP_007136210.1\|	2.7e-52	70.8	hypothetical protein PHAVU_009...	[more]
gi\|729296598\|ref\|XP_010544234.1\|	2.3e-51	69.5	PREDICTED: heavy metal-associa...	[more]

BLAST of Spo20357.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0J8BEB9_BETVU	7.3e-65	84.3	Uncharacterized protein OS=Bet...	[more]
A0A0K9QK80_SPIOL	3.0e-58	100.	Uncharacterized protein (Fragm...	[more]
V7ARL8_PHAVU	1.9e-52	70.8	Uncharacterized protein OS=Pha...	[more]
A0A0B0MKC0_GOSAR	1.6e-51	69.5	Superoxide dismutase 1 copper ...	[more]
A0A0D2QGZ4_GOSRA	3.5e-51	68.8	Uncharacterized protein OS=Gos...	[more]

BLAST of Spo20357.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
HIP22_ARATH	8.4e-38	55.8	Heavy metal-associated isopren...	[more]
HIP20_ARATH	8.4e-38	50.6	Heavy metal-associated isopren...	[more]
HIP26_ARATH	9.6e-34	49.0	Heavy metal-associated isopren...	[more]
HIP27_ARATH	1.7e-30	50.3	Heavy metal-associated isopren...	[more]
HIP25_ARATH	1.3e-25	39.8	Heavy metal-associated isopren...	[more]

BLAST of Spo20357.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
AT4G08570.1	3.4e-53	66.8	Heavy metal transport/detoxifi...	[more]
AT4G39700.1	2.2e-44	57.2	Heavy metal transport/detoxifi...	[more]
AT1G71050.1	4.7e-39	50.6	Heavy metal transport/detoxifi...	[more]
AT1G22990.1	4.7e-39	55.8	Heavy metal transport/detoxifi...	[more]
AT4G38580.1	5.4e-35	49.0	farnesylated protein 6	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 33..87 score: 8.5
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 28..91 score: 13
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 27..93 score: 4.32
None	No IPR available	GENE3D	3.30.70.100		coord: 26..93 score: 5.6
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 14..146 score: 1.6
None	No IPR available	PANTHER	PTHR22814:SF84	HEAVY-METAL-ASSOCIATED DOMAIN-CONTAINING PROTEIN	coord: 14..146 score: 1.6

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030001	metal ion transport
biological_process	GO:0019285	glycine betaine biosynthetic process from choline
biological_process	GO:0006810	transport
biological_process	GO:0009409	response to cold
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006012	galactose metabolic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0019745	pentacyclic triterpenoid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019439	aromatic compound catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005634	nucleus
cellular_component	GO:0000785	chromatin
cellular_component	GO:0005667	transcription factor complex
cellular_component	GO:0005622	intracellular
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016708	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of two atoms of oxygen into one donor
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008289	lipid binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0019133	choline monooxygenase activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003682	chromatin binding
molecular_function	GO:0047216	inositol 3-alpha-galactosyltransferase activity
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo10280	0.72	Barchart \| Table
Spo03551	0.69	Barchart \| Table
Spo27132	0.69	Barchart \| Table
Spo10957	0.68	Barchart \| Table
Spo01697	0.67	Barchart \| Table
Spo18174	0.67	Barchart \| Table
Spo13645	0.66	Barchart \| Table
Spo01230	0.66	Barchart \| Table
Spo17665	0.66	Barchart \| Table
Spo20891	0.66	Barchart \| Table
Spo26520	0.66	Barchart \| Table
Spo06012	0.65	Barchart \| Table
Spo24509	0.65	Barchart \| Table