Spo08314.1 (mRNA) | SpinachBase

Overview


Name	Spo08314.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Kinase family protein
Location	SpoScf_02868 : 1502 .. 2017 (+)
Sequence length	516

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTTTTGTGGCTTTGAAGATTCAAAAAAGTGCACCGCAGTTTGCTCAGGCTGCCCTTCATGAAATTGAAGTCCTTTCAGCGATTGCTGATGGAGAACCAACTCATTCCAAGTATGTAGTTCGATTGATCGATAACTTCAAGCATACGGGGCCAAATGGGCAGCACCTGTGCATGGTTCTTGAGTTTCTAGGGGATAGCTTGCTTCGTTTAATTAGATACAACCGTCACAAAGGTATGGATCTGAACAAAGTTAGAGAAATTTGCAAATGTGTTTTGATGGGCTTGGATTATTTGCATAGGGAGCTTAATATAATCCACACAGACCTAAAGCCGGAAAACATTCTTCTTGTCTCTCCCATTGACCCCTCTAAAGATCCAGTAAAATCTGGATTTACCCCAATTCTTGAGAAGCCTGAAGGAGGAAACCATAATGGTGGAGGAGTTTCAATGAGTTTNTTACCCCAATTCTTGAGAAGCCTGAAGGAGGAAACCATAATGGTGGAGGAGTTTCAATGA

mRNA sequence

AGTTTTGTGGCTTTGAAGATTCAAAAAAGTGCACCGCAGTTTGCTCAGGCTGCCCTTCATGAAATTGAAGTCCTTTCAGCGATTGCTGATGGAGAACCAACTCATTCCAAGTATGTAGTTCGATTGATCGATAACTTCAAGCATACGGGGCCAAATGGGCAGCACCTGTGCATGGTTCTTGAGTTTCTAGGGGATAGCTTGCTTCGTTTAATTAGATACAACCGTCACAAAGGTATGGATCTGAACAAAGTTAGAGAAATTTGCAAATGTGTTTTGATGGGCTTGGATTATTTGCATAGGGAGCTTAATATAATCCACACAGACCTAAAGCCGGAAAACATTCTTCTTGTCTCTCCCATTGACCCCTCTAAAGATCCAGTAAAATCTGGATTTACCCCAATTCTTGAGAAGCCTGAAGGAGGAAACCATAATGGTGGAGGAGTTTCAATGAGTTTNTTACCCCAATTCTTGAGAAGCCTGAAGGAGGAAACCATAATGGTGGAGGAGTTTCAATGA

Coding sequence (CDS)

AGTTTTGTGGCTTTGAAGATTCAAAAAAGTGCACCGCAGTTTGCTCAGGCTGCCCTTCATGAAATTGAAGTCCTTTCAGCGATTGCTGATGGAGAACCAACTCATTCCAAGTATGTAGTTCGATTGATCGATAACTTCAAGCATACGGGGCCAAATGGGCAGCACCTGTGCATGGTTCTTGAGTTTCTAGGGGATAGCTTGCTTCGTTTAATTAGATACAACCGTCACAAAGGTATGGATCTGAACAAAGTTAGAGAAATTTGCAAATGTGTTTTGATGGGCTTGGATTATTTGCATAGGGAGCTTAATATAATCCACACAGACCTAAAGCCGGAAAACATTCTTCTTGTCTCTCCCATTGACCCCTCTAAAGATCCAGTAAAATCTGGATTTACCCCAATTCTTGAGAAGCCTGAAGGAGGAAACCATAATGGTGGAGGAGTTTCAATGAGTTTNTTACCCCAATTCTTGAGAAGCCTGAAGGAGGAAACCATAATGGTGGAGGAGTTTCAATGA

Protein sequence

SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLEFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPIDPSKDPVKSGFTPILEKPEGGNHNGGGVSMSXLPQFLRSLKEETIMVEEFQ

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08314	Spo08314	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08314.1	Spo08314.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08314.1.exon.1	Spo08314.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08314.1.CDS.1	Spo08314.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo08314.1 vs. NCBI nr
Match: gi|902225590|gb|KNA20111.1| (hypothetical protein SOVF_055320 [Spinacia oleracea])

HSP 1 Score: 312.4 bits (799), Expect = 4.900e-82
Identity = 152/158 (96.20%), Postives = 155/158 (98.10%), Query Frame = 1


		  
Query: 1   SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL 60
           SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL
Sbjct: 61  SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL 120

Query: 61  EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI 120
           EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI
Sbjct: 121 EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI 180

Query: 121 DPSKDPVKSGFTPILEKPEGGNHNGGGVSMSXLPQFLR 159
           DPSKDPVKSGFTPILEKPEGGNHNGGGVSMS + + L+
Sbjct: 181 DPSKDPVKSGFTPILEKPEGGNHNGGGVSMSLIEKKLK 218

[Close]

BLAST of Spo08314.1 vs. NCBI nr
Match: gi|731339000|ref|XP_010680616.1| (PREDICTED: SRSF protein kinase 1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 287.7 bits (735), Expect = 1.300e-74
Identity = 138/157 (87.90%), Postives = 150/157 (95.54%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           FVALKIQKSAPQFAQAALHEIE+LSAIADG+ THSK VVRLID+FKHTGPNGQHLCMVLE
Sbjct: 63  FVALKIQKSAPQFAQAALHEIEILSAIADGDLTHSKCVVRLIDHFKHTGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           +LGDSLLRLIRYNRHKGM+LNKVREIC+C+L+GLDYLHRE NIIHTDLKPENILL SPID
Sbjct: 123 YLGDSLLRLIRYNRHKGMNLNKVREICRCILIGLDYLHRERNIIHTDLKPENILLNSPID 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGGGVSMSXLPQFLR 159
           PSKDPVKSGFTPILE+PEGGNHNGGGVSMS + + L+
Sbjct: 183 PSKDPVKSGFTPILERPEGGNHNGGGVSMSLIEKKLK 219

[Close]

BLAST of Spo08314.1 vs. NCBI nr
Match: gi|590701820|ref|XP_007046486.1| (Kinase superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 253.8 bits (647), Expect = 2.100e-64
Identity = 120/145 (82.76%), Postives = 136/145 (93.79%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSA QFAQAALHEIEVLS+IADG+P++SK +VRLID+FKHTGPNGQHLCMVLE
Sbjct: 63  YVALKIQKSAQQFAQAALHEIEVLSSIADGDPSNSKCIVRLIDHFKHTGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           FLGDSLLRLIR+NR+KG++LNKVREICKC+LMGLDYLHREL IIHTDLKPENILL S ID
Sbjct: 123 FLGDSLLRLIRFNRYKGIELNKVREICKCILMGLDYLHRELGIIHTDLKPENILLFSTID 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGG 147
           P+KDP++SG TP LE+PEGG  NGG
Sbjct: 183 PTKDPIRSGLTPTLERPEGGTLNGG 207

[Close]

BLAST of Spo08314.1 vs. NCBI nr
Match: gi|661894782|emb|CDP01781.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 253.8 bits (647), Expect = 2.100e-64
Identity = 118/149 (79.19%), Postives = 137/149 (91.95%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSAPQFAQAALHEIE+LSAIADG+ ++SKYV+RL+D+FKH GPNGQHLCMVLE
Sbjct: 63  YVALKIQKSAPQFAQAALHEIEILSAIADGDASNSKYVIRLVDHFKHAGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           FLGDSLLRLI+YNR+KG++LNKVREICKC+L GLDYLHREL IIHTDLKPENILL S I+
Sbjct: 123 FLGDSLLRLIKYNRYKGLELNKVREICKCILTGLDYLHRELGIIHTDLKPENILLCSTIN 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGGGVSM 151
           PSKDPVKSG TPILE+PEG  + G  +++
Sbjct: 183 PSKDPVKSGMTPILERPEGNQNGGAAINL 211

[Close]

BLAST of Spo08314.1 vs. NCBI nr
Match: gi|590701824|ref|XP_007046487.1| (Kinesin motor family protein isoform 2 [Theobroma cacao])

HSP 1 Score: 253.8 bits (647), Expect = 2.100e-64
Identity = 120/145 (82.76%), Postives = 136/145 (93.79%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSA QFAQAALHEIEVLS+IADG+P++SK +VRLID+FKHTGPNGQHLCMVLE
Sbjct: 63  YVALKIQKSAQQFAQAALHEIEVLSSIADGDPSNSKCIVRLIDHFKHTGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           FLGDSLLRLIR+NR+KG++LNKVREICKC+LMGLDYLHREL IIHTDLKPENILL S ID
Sbjct: 123 FLGDSLLRLIRFNRYKGIELNKVREICKCILMGLDYLHRELGIIHTDLKPENILLFSTID 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGG 147
           P+KDP++SG TP LE+PEGG  NGG
Sbjct: 183 PTKDPIRSGLTPTLERPEGGTLNGG 207

[Close]

BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RKT6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_055320 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 3.400e-82
Identity = 152/158 (96.20%), Postives = 155/158 (98.10%), Query Frame = 1


		  
Query: 1   SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL 60
           SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL
Sbjct: 61  SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL 120

Query: 61  EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI 120
           EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI
Sbjct: 121 EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI 180

Query: 121 DPSKDPVKSGFTPILEKPEGGNHNGGGVSMSXLPQFLR 159
           DPSKDPVKSGFTPILEKPEGGNHNGGGVSMS + + L+
Sbjct: 181 DPSKDPVKSGFTPILEKPEGGNHNGGGVSMSLIEKKLK 218

[Close]

BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CA01_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g135760 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 9.100e-75
Identity = 138/157 (87.90%), Postives = 150/157 (95.54%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           FVALKIQKSAPQFAQAALHEIE+LSAIADG+ THSK VVRLID+FKHTGPNGQHLCMVLE
Sbjct: 63  FVALKIQKSAPQFAQAALHEIEILSAIADGDLTHSKCVVRLIDHFKHTGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           +LGDSLLRLIRYNRHKGM+LNKVREIC+C+L+GLDYLHRE NIIHTDLKPENILL SPID
Sbjct: 123 YLGDSLLRLIRYNRHKGMNLNKVREICRCILIGLDYLHRERNIIHTDLKPENILLNSPID 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGGGVSMSXLPQFLR 159
           PSKDPVKSGFTPILE+PEGGNHNGGGVSMS + + L+
Sbjct: 183 PSKDPVKSGFTPILERPEGGNHNGGGVSMSLIEKKLK 219

[Close]

BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
Match: A0A061DKZ9_THECC (Kinase superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_000057 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.500e-64
Identity = 120/145 (82.76%), Postives = 136/145 (93.79%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSA QFAQAALHEIEVLS+IADG+P++SK +VRLID+FKHTGPNGQHLCMVLE
Sbjct: 63  YVALKIQKSAQQFAQAALHEIEVLSSIADGDPSNSKCIVRLIDHFKHTGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           FLGDSLLRLIR+NR+KG++LNKVREICKC+LMGLDYLHREL IIHTDLKPENILL S ID
Sbjct: 123 FLGDSLLRLIRFNRYKGIELNKVREICKCILMGLDYLHRELGIIHTDLKPENILLFSTID 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGG 147
           P+KDP++SG TP LE+PEGG  NGG
Sbjct: 183 PTKDPIRSGLTPTLERPEGGTLNGG 207

[Close]

BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
Match: A0A061DF19_THECC (Kinesin motor family protein isoform 2 OS=Theobroma cacao GN=TCM_000057 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.500e-64
Identity = 120/145 (82.76%), Postives = 136/145 (93.79%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSA QFAQAALHEIEVLS+IADG+P++SK +VRLID+FKHTGPNGQHLCMVLE
Sbjct: 63  YVALKIQKSAQQFAQAALHEIEVLSSIADGDPSNSKCIVRLIDHFKHTGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           FLGDSLLRLIR+NR+KG++LNKVREICKC+LMGLDYLHREL IIHTDLKPENILL S ID
Sbjct: 123 FLGDSLLRLIRFNRYKGIELNKVREICKCILMGLDYLHRELGIIHTDLKPENILLFSTID 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGG 147
           P+KDP++SG TP LE+PEGG  NGG
Sbjct: 183 PTKDPIRSGLTPTLERPEGGTLNGG 207

[Close]

BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
Match: A0A068TZW5_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00036940001 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.500e-64
Identity = 118/149 (79.19%), Postives = 137/149 (91.95%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSAPQFAQAALHEIE+LSAIADG+ ++SKYV+RL+D+FKH GPNGQHLCMVLE
Sbjct: 63  YVALKIQKSAPQFAQAALHEIEILSAIADGDASNSKYVIRLVDHFKHAGPNGQHLCMVLE 122

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           FLGDSLLRLI+YNR+KG++LNKVREICKC+L GLDYLHREL IIHTDLKPENILL S I+
Sbjct: 123 FLGDSLLRLIKYNRYKGLELNKVREICKCILTGLDYLHRELGIIHTDLKPENILLCSTIN 182

Query: 122 PSKDPVKSGFTPILEKPEGGNHNGGGVSM 151
           PSKDPVKSG TPILE+PEG  + G  +++
Sbjct: 183 PSKDPVKSGMTPILERPEGNQNGGAAINL 211

[Close]

BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
Match: SRPK_PHYPO (Serine/threonine-protein kinase SRPK OS=Physarum polycephalum PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.100e-28
Identity = 61/114 (53.51%), Postives = 84/114 (73.68%), Query Frame = 1


		  
Query: 3   VALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLEF 62
           VALKI KSA  + +AA  EI +L  I++G+P     VV+L+D+F HTGP+G+H+CMV E 
Sbjct: 83  VALKIVKSASHYREAAEDEIHLLQTISEGDPESKYCVVKLLDSFLHTGPHGKHICMVFEK 142

Query: 63  LGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILL 117
           LG +LL LI+ + +KG+ L  V+ + K +L+GLDYLH +  IIHTDLKPEN+LL
Sbjct: 143 LGSNLLDLIKLHNYKGIPLPLVKCMTKQILIGLDYLHTKCKIIHTDLKPENVLL 196

[Close]

BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
Match: DSK1_SCHPO (Protein kinase dsk1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=dsk1 PE=3 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 3.800e-26
Identity = 55/116 (47.41%), Postives = 81/116 (69.83%), Query Frame = 1


		  
Query: 3   VALKIQKSAPQFAQAALHEIEVLSAIADGEPTH--SKYVVRLIDNFKHTGPNGQHLCMVL 62
           VALK+ +SA  + + ++ EI +L  I +G+  H   K+++ L+D F H GPNG H+CMV 
Sbjct: 107 VALKVVRSAEHYRETSIDEIRILQKIREGDEKHLGKKHIISLLDYFVHRGPNGAHVCMVF 166

Query: 63  EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILL 117
           E LG++LL LI+   H+G+ +  V++I   +L+ LDYLHRE  IIHTDLKPEN+L+
Sbjct: 167 EVLGENLLSLIQSYGHRGVPVGIVKQIAYQLLIALDYLHRECGIIHTDLKPENVLI 222

[Close]

BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
Match: SKY1_DICDI (Probable serine/threonine-protein kinase sky1 OS=Dictyostelium discoideum GN=sky1 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.000e-26
Identity = 58/119 (48.74%), Postives = 83/119 (69.75%), Query Frame = 1


		  
Query: 3   VALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLEF 62
           VALKI +SA  +++ A  EI++L+AI+       K V RL+D+F H GPNG+H CMV E 
Sbjct: 194 VALKIVRSARTYSETAEDEIKILNAISKYN-AQDKCVARLLDHFTHRGPNGRHYCMVFEL 253

Query: 63  LGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 122
           LG++LL LI+++R++GM +  V+ + K  L+ LDY+H +  IIHTDLKPEN+LL    D
Sbjct: 254 LGNNLLDLIKHHRYRGMPITLVKTLMKQTLIALDYIHTKCKIIHTDLKPENVLLEKSFD 311

[Close]

BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
Match: SPK1_CAEBR (Serine/threonine-protein kinase spk-1 OS=Caenorhabditis briggsae GN=spk-1 PE=3 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 1.200e-24
Identity = 58/117 (49.57%), Postives = 82/117 (70.09%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAI--ADGEPTHSKYVVRLIDNFKHTGPNGQHLCMV 61
           F A+KI KSA  + +AAL EI++L ++  AD E T    VV+L+D F  TG NGQH+ MV
Sbjct: 520 FTAMKIVKSAEHYTEAALDEIKLLLSVRGADPEDTGCHKVVQLLDEFTVTGINGQHVAMV 579

Query: 62  LEFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILL 117
            E LG +LL+LI  + ++G+ L +VR+ICK +L  L Y+H +  IIHTD+KPEN+L+
Sbjct: 580 FEVLGCNLLKLIIRSNYRGLHLEQVRKICKQILEALRYMHEKCGIIHTDIKPENVLI 636

[Close]

BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
Match: SRPK3_MOUSE (SRSF protein kinase 3 OS=Mus musculus GN=Srpk3 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.100e-24
Identity = 57/117 (48.72%), Postives = 82/117 (70.09%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKY--VVRLIDNFKHTGPNGQHLCMV 61
           FVALK+ KSA  + + A+ EI++L  + D +P+  K   +V+LID+F+ +G NG H+CMV
Sbjct: 103 FVALKVVKSAGHYTETAVDEIKLLKCVRDSDPSDPKRETIVQLIDDFRISGVNGVHVCMV 162

Query: 62  LEFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILL 117
           LE LG  LL+ I  + ++G+ +  V+ I + VL GLDYLH +  IIHTD+KPENILL
Sbjct: 163 LEVLGHQLLKWIIKSNYQGLPVPCVKSIVRQVLHGLDYLHTKCKIIHTDIKPENILL 219

[Close]

BLAST of Spo08314.1 vs. TAIR (Arabidopsis)
Match: AT2G17530.1 (Protein kinase superfamily protein)

HSP 1 Score: 238.8 bits (608), Expect = 2.500e-63
Identity = 114/145 (78.62%), Postives = 132/145 (91.03%), Query Frame = 1


		  
Query: 1   SFVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVL 60
           ++VALKIQKSA QFAQAALHEIE+L A ADG+P ++K V+RLID+FKH GPNGQHLCMVL
Sbjct: 62  NYVALKIQKSALQFAQAALHEIELLQAAADGDPENTKCVIRLIDDFKHAGPNGQHLCMVL 121

Query: 61  EFLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPI 120
           EFLGDSLLRLI+YNR+KGM+L+KVREICKC+L GLDYLHREL +IH+DLKPENILL S I
Sbjct: 122 EFLGDSLLRLIKYNRYKGMELSKVREICKCILTGLDYLHRELGMIHSDLKPENILLCSTI 181

Query: 121 DPSKDPVKSGFTPILEKPEGGNHNG 146
           DP+KDP++SG TPILEKPE GN NG
Sbjct: 182 DPAKDPIRSGLTPILEKPE-GNQNG 205

[Close]

BLAST of Spo08314.1 vs. TAIR (Arabidopsis)
Match: AT4G35500.2 (Protein kinase superfamily protein)

HSP 1 Score: 224.9 bits (572), Expect = 3.700e-59
Identity = 109/148 (73.65%), Postives = 128/148 (86.49%), Query Frame = 1


		  
Query: 11  APQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLEFLGDSLLRL 70
           A QFAQAALHEIE LSA ADG+   +K VVRLID+FKH+GPNGQHLCMVLEFLGDSLLRL
Sbjct: 73  AQQFAQAALHEIEFLSAAADGDLDKTKCVVRLIDHFKHSGPNGQHLCMVLEFLGDSLLRL 132

Query: 71  IRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPIDPSKDPVKSG 130
           IRYN++KG+ LNKVREIC+C+L GLDYLHREL +IH+DLKPENILL S IDP+KDPV+SG
Sbjct: 133 IRYNQYKGLKLNKVREICRCILTGLDYLHRELGMIHSDLKPENILLCSTIDPAKDPVRSG 192

Query: 131 FTPILEKPEGGNHNGGGVSMSXLPQFLR 159
            TP+LEKPE GN NGG  +M+ + + L+
Sbjct: 193 LTPLLEKPE-GNANGGASTMNLIEKKLK 219

[Close]

BLAST of Spo08314.1 vs. TAIR (Arabidopsis)
Match: AT3G53030.1 (ser/arg-rich protein kinase 4)

HSP 1 Score: 172.6 bits (436), Expect = 2.200e-43
Identity = 79/134 (58.96%), Postives = 109/134 (81.34%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALK+QKSA  + +AA+ EI +L  IA+G+   +K VV+L+D+FKH+GPNGQH+CMV E
Sbjct: 67  YVALKVQKSAQHYTEAAMDEITILQQIAEGDTDDTKCVVKLLDHFKHSGPNGQHVCMVFE 126

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           +LGD+LL LI+Y+ ++G+ +  V+EIC  +L+GLDYLH++L+IIHTDLKPEN+LL S ID
Sbjct: 127 YLGDNLLTLIKYSDYRGLPIPMVKEICYHMLVGLDYLHKQLSIIHTDLKPENVLLPSTID 186

Query: 122 PSKDPVKSGFTPIL 136
           PSKDP KSG   +L
Sbjct: 187 PSKDPRKSGAPLVL 200

[Close]

BLAST of Spo08314.1 vs. TAIR (Arabidopsis)
Match: AT3G44850.1 (Protein kinase superfamily protein)

HSP 1 Score: 170.6 bits (431), Expect = 8.200e-43
Identity = 77/134 (57.46%), Postives = 110/134 (82.09%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALK+QKSA  + +AA+ EI++L  IA+G+    K VV+L+D+FKHTGPNG+H+CMV E
Sbjct: 70  YVALKVQKSAQHYTEAAMDEIKILKQIAEGDSGDKKCVVKLLDHFKHTGPNGKHVCMVFE 129

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           +LGD+LL +I+Y+ ++G+ L+ V+E+C  +L+GLDYLHREL+IIHTDLKPEN+LL+S ID
Sbjct: 130 YLGDNLLSVIKYSDYRGVPLHMVKELCFHILVGLDYLHRELSIIHTDLKPENVLLLSTID 189

Query: 122 PSKDPVKSGFTPIL 136
           PS+D  +SG   +L
Sbjct: 190 PSRDVRRSGVPLVL 203

[Close]

BLAST of Spo08314.1 vs. TAIR (Arabidopsis)
Match: AT5G22840.1 (Protein kinase superfamily protein)

HSP 1 Score: 169.9 bits (429), Expect = 1.400e-42
Identity = 79/134 (58.96%), Postives = 106/134 (79.10%), Query Frame = 1


		  
Query: 2   FVALKIQKSAPQFAQAALHEIEVLSAIADGEPTHSKYVVRLIDNFKHTGPNGQHLCMVLE 61
           +VALKIQKSA  + +AA+ EI++L  IA+G+    K VV+L+D+FKH GPNGQH+CMV E
Sbjct: 70  YVALKIQKSAQHYTEAAMDEIKILKQIAEGDAEDKKCVVKLLDHFKHAGPNGQHVCMVFE 129

Query: 62  FLGDSLLRLIRYNRHKGMDLNKVREICKCVLMGLDYLHRELNIIHTDLKPENILLVSPID 121
           +LGD+LL +I+Y+ ++G+ L+ V+EIC  +L+GLDYLHREL+IIHTD+KPENILL S ID
Sbjct: 130 YLGDNLLSVIKYSDYRGVPLHMVKEICFHILVGLDYLHRELSIIHTDIKPENILLCSTID 189

Query: 122 PSKDPVKSGFTPIL 136
           P  D  KSG   +L
Sbjct: 190 PEADARKSGIPLVL 203

The following BLAST results are available for this feature:

BLAST of Spo08314.1 vs. NCBI nr
BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
BLAST of Spo08314.1 vs. TAIR (Arabidopsis)

BLAST of Spo08314.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902225590\|gb\|KNA20111.1\|	4.9e-82	96.2	hypothetical protein SOVF_0553...	[more]
gi\|731339000\|ref\|XP_010680616.1\|	1.3e-74	87.9	PREDICTED: SRSF protein kinase...	[more]
gi\|590701820\|ref\|XP_007046486.1\|	2.1e-64	82.7	Kinase superfamily protein iso...	[more]
gi\|661894782\|emb\|CDP01781.1\|	2.1e-64	79.1	unnamed protein product [Coffe...	[more]
gi\|590701824\|ref\|XP_007046487.1\|	2.1e-64	82.7	Kinesin motor family protein i...	[more]

BLAST of Spo08314.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RKT6_SPIOL	3.4e-82	96.2	Uncharacterized protein OS=Spi...	[more]
A0A0J8CA01_BETVU	9.1e-75	87.9	Uncharacterized protein OS=Bet...	[more]
A0A061DKZ9_THECC	1.5e-64	82.7	Kinase superfamily protein iso...	[more]
A0A061DF19_THECC	1.5e-64	82.7	Kinesin motor family protein i...	[more]
A0A068TZW5_COFCA	1.5e-64	79.1	Uncharacterized protein OS=Cof...	[more]

BLAST of Spo08314.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
SRPK_PHYPO	3.1e-28	53.5	Serine/threonine-protein kinas...	[more]
DSK1_SCHPO	3.8e-26	47.4	Protein kinase dsk1 OS=Schizos...	[more]
SKY1_DICDI	5.0e-26	48.7	Probable serine/threonine-prot...	[more]
SPK1_CAEBR	1.2e-24	49.5	Serine/threonine-protein kinas...	[more]
SRPK3_MOUSE	2.1e-24	48.7	SRSF protein kinase 3 OS=Mus m...	[more]

BLAST of Spo08314.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT2G17530.1	2.5e-63	78.6	Protein kinase superfamily pro...	[more]
AT4G35500.2	3.7e-59	73.6	Protein kinase superfamily pro...	[more]
AT3G53030.1	2.2e-43	58.9	ser/arg-rich protein kinase 4	[more]
AT3G44850.1	8.2e-43	57.4	Protein kinase superfamily pro...	[more]
AT5G22840.1	1.4e-42	58.9	Protein kinase superfamily pro...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 4..117 score: 1.6
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..171 score: 1.
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..171 score: 1
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 104..116 scor
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 2..118 score: 2.63
None	No IPR available	GENE3D	1.10.510.10		coord: 58..118 score: 4.7
None	No IPR available	GENE3D	3.30.200.20		coord: 2..57 score: 4.3
None	No IPR available	PANTHER	PTHR24055	MITOGEN-ACTIVATED PROTEIN KINASE	coord: 1..125 score: 7.8
None	No IPR available	PANTHER	PTHR24055:SF138	PROTEIN KINASE FAMILY PROTEIN	coord: 1..125 score: 7.8

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity