Spo26994.1 (mRNA) | SpinachBase

Overview


Name	Spo26994.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Mitochondrial import receptor subunit TOM9-2 (Mitochondrial import receptor subunit TOM22 homolog 2) (Translocase of outer membrane 22 kDa subunit homolog 2) (Translocase of outer membrane 9 kDa subunit TOM9-2)
Location	SpoScf_03596 : 19903 .. 20205 (-)
Sequence length	303

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCAAACAGAAAGAGAATAGGATCAAGCAGCGACAATGGAGGCGGAGTACTAGCAAGAGTGACAAGCACAGTTTCAGATTCACCAATCTTCCAAAAAGGGGTACAATCTGCTAGCGATGTGGCCGGTGTCACCAAGAAGCTGCTCAAGAGCACCGGCAAAGCTGCTTGGATCGTCGGCACCACTTTCCTCGTCCTTGCTCTCCCGCTCATCATCGAGCTCGAGCGCGATGGTCAGTTGGATGCTATAGAGCTTCAGCAGGCTAGTCTTCTCGGCACCCCTCCCCCTGCCGGCCATTAA

mRNA sequence

ATGGCGTCAAACAGAAAGAGAATAGGATCAAGCAGCGACAATGGAGGCGGAGTACTAGCAAGAGTGACAAGCACAGTTTCAGATTCACCAATCTTCCAAAAAGGGGTACAATCTGCTAGCGATGTGGCCGGTGTCACCAAGAAGCTGCTCAAGAGCACCGGCAAAGCTGCTTGGATCGTCGGCACCACTTTCCTCGTCCTTGCTCTCCCGCTCATCATCGAGCTCGAGCGCGATGGTCAGTTGGATGCTATAGAGCTTCAGCAGGCTAGTCTTCTCGGCACCCCTCCCCCTGCCGGCCATTAA

Coding sequence (CDS)

ATGGCGTCAAACAGAAAGAGAATAGGATCAAGCAGCGACAATGGAGGCGGAGTACTAGCAAGAGTGACAAGCACAGTTTCAGATTCACCAATCTTCCAAAAAGGGGTACAATCTGCTAGCGATGTGGCCGGTGTCACCAAGAAGCTGCTCAAGAGCACCGGCAAAGCTGCTTGGATCGTCGGCACCACTTTCCTCGTCCTTGCTCTCCCGCTCATCATCGAGCTCGAGCGCGATGGTCAGTTGGATGCTATAGAGCTTCAGCAGGCTAGTCTTCTCGGCACCCCTCCCCCTGCCGGCCATTAA

Protein sequence

MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo26994	Spo26994	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo26994.1	Spo26994.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo26994.1.CDS.1	Spo26994.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo26994.1.exon.1	Spo26994.1.exon.1	exon

Homology

[Close]

BLAST of Spo26994.1 vs. NCBI nr
Match: gi|902184558|gb|KNA10312.1| (hypothetical protein SOVF_145450 [Spinacia oleracea])

HSP 1 Score: 189.9 bits (481), Expect = 2.200e-45
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1


		  
Query: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 100

[Close]

BLAST of Spo26994.1 vs. NCBI nr
Match: gi|902183133|gb|KNA10063.1| (hypothetical protein SOVF_147920 [Spinacia oleracea])

HSP 1 Score: 184.1 bits (466), Expect = 1.200e-43
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 1


		  
Query: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MAS RKRIGSSSDNGGGVLARVT+TVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA H
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 100

[Close]

BLAST of Spo26994.1 vs. NCBI nr
Match: gi|731372562|ref|XP_010666386.1| (PREDICTED: mitochondrial import receptor subunit TOM9-2-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 165.2 bits (417), Expect = 5.700e-38
Identity = 89/98 (90.82%), Postives = 93/98 (94.90%), Query Frame = 1


		  
Query: 2   ASNRKRIGSSSDNG-GGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 61
           +S RKRIGSSSDNG GGV+ARVTSTVSDSPIFQKGVQSAS  A +TKKLLKSTGKAAWIV
Sbjct: 4   SSGRKRIGSSSDNGGGGVIARVTSTVSDSPIFQKGVQSASGAADITKKLLKSTGKAAWIV 63

Query: 62  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA 99
           GTTFLVLALPLIIELERDGQLDA+ELQQASLLGTPPPA
Sbjct: 64  GTTFLVLALPLIIELERDGQLDALELQQASLLGTPPPA 101

[Close]

BLAST of Spo26994.1 vs. NCBI nr
Match: gi|460389672|ref|XP_004240474.1| (PREDICTED: mitochondrial import receptor subunit TOM9-2-like [Solanum lycopersicum])

HSP 1 Score: 116.7 bits (291), Expect = 2.300e-23
Identity = 58/93 (62.37%), Postives = 75/93 (80.65%), Query Frame = 1


		  
Query: 7   RIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLV 66
           R G+ +  G G+L+R +S++S+SPI  +G ++ASDVA V KKL KSTGKAAWI GTTFL+
Sbjct: 13  RPGAGAGAGDGILSRFSSSISESPIVYQGKRAASDVAFVAKKLFKSTGKAAWIAGTTFLI 72

Query: 67  LALPLIIELERDGQLDAIELQQASLLGTPPPAG 100
           L +PLIIE++RD QL  +E+QQASLLG PPPAG
Sbjct: 73  LVVPLIIEMDRDAQLTELEMQQASLLGAPPPAG 105

[Close]

BLAST of Spo26994.1 vs. NCBI nr
Match: gi|698513962|ref|XP_009801898.1| (PREDICTED: mitochondrial import receptor subunit TOM9-2-like [Nicotiana sylvestris])

HSP 1 Score: 115.2 bits (287), Expect = 6.800e-23
Identity = 56/84 (66.67%), Postives = 71/84 (84.52%), Query Frame = 1


		  
Query: 15  GGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLVLALPLIIE 74
           G G+++RV+S++S SPI  +G ++ASD A + KKLLKSTGKAAWI GTTFL+L +PLIIE
Sbjct: 19  GDGIISRVSSSISQSPIVYQGKRAASDAALIAKKLLKSTGKAAWIAGTTFLILVVPLIIE 78

Query: 75  LERDGQLDAIELQQASLLGTPPPA 99
           ++RD QL  +ELQQASLLGTPPPA
Sbjct: 79  MDRDAQLTELELQQASLLGTPPPA 102

[Close]

BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QSN9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_145450 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.500e-45
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1


		  
Query: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 100

[Close]

BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QTM4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_147920 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 8.300e-44
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 1


		  
Query: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MAS RKRIGSSSDNGGGVLARVT+TVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA H
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 100

[Close]

BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B4L3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_001700 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 4.000e-38
Identity = 89/98 (90.82%), Postives = 93/98 (94.90%), Query Frame = 1


		  
Query: 2   ASNRKRIGSSSDNG-GGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 61
           +S RKRIGSSSDNG GGV+ARVTSTVSDSPIFQKGVQSAS  A +TKKLLKSTGKAAWIV
Sbjct: 4   SSGRKRIGSSSDNGGGGVIARVTSTVSDSPIFQKGVQSASGAADITKKLLKSTGKAAWIV 63

Query: 62  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA 99
           GTTFLVLALPLIIELERDGQLDA+ELQQASLLGTPPPA
Sbjct: 64  GTTFLVLALPLIIELERDGQLDALELQQASLLGTPPPA 101

[Close]

BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
Match: K4C3J2_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.600e-23
Identity = 58/93 (62.37%), Postives = 75/93 (80.65%), Query Frame = 1


		  
Query: 7   RIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLV 66
           R G+ +  G G+L+R +S++S+SPI  +G ++ASDVA V KKL KSTGKAAWI GTTFL+
Sbjct: 13  RPGAGAGAGDGILSRFSSSISESPIVYQGKRAASDVAFVAKKLFKSTGKAAWIAGTTFLI 72

Query: 67  LALPLIIELERDGQLDAIELQQASLLGTPPPAG 100
           L +PLIIE++RD QL  +E+QQASLLG PPPAG
Sbjct: 73  LVVPLIIEMDRDAQLTELEMQQASLLGAPPPAG 105

[Close]

BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
Match: A0A0V0GW57_SOLCH (Putative mitochondrial import receptor subunit TOM9-2-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 8.100e-23
Identity = 55/85 (64.71%), Postives = 71/85 (83.53%), Query Frame = 1


		  
Query: 15  GGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLVLALPLIIE 74
           G G+++R +S++S+SPI  +G ++ASDVA V KKL KSTGKAAWI GTTFL+L +PLIIE
Sbjct: 17  GDGIISRFSSSISESPIVYQGKRAASDVAFVAKKLFKSTGKAAWIAGTTFLILVVPLIIE 76

Query: 75  LERDGQLDAIELQQASLLGTPPPAG 100
           ++RD QL  +E+QQASLLG PPPAG
Sbjct: 77  MDRDAQLTELEMQQASLLGAPPPAG 101

[Close]

BLAST of Spo26994.1 vs. ExPASy Swiss-Prot
Match: TOM92_ARATH (Mitochondrial import receptor subunit TOM9-2 OS=Arabidopsis thaliana GN=TOM9-2 PE=1 SV=3)

HSP 1 Score: 98.2 bits (243), Expect = 5.400e-20
Identity = 53/93 (56.99%), Postives = 69/93 (74.19%), Query Frame = 1


		  
Query: 6  KRIGSSSDNGG--GVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTT 65
          KRIG+    GG   +LAR+    S+S I  +G ++A D   V+KKLL+STGKAAWI GTT
Sbjct: 4  KRIGAGKSGGGDPNILARI----SNSEIVSQGRRAAGDAVEVSKKLLRSTGKAAWIAGTT 63

Query: 66 FLVLALPLIIELERDGQLDAIELQQASLLGTPP 97
          FL+L +PLIIE++R+ Q++ IELQQASLLG PP
Sbjct: 64 FLILVVPLIIEMDREAQINEIELQQASLLGAPP 92

[Close]

BLAST of Spo26994.1 vs. ExPASy Swiss-Prot
Match: TOM91_ARATH (Mitochondrial import receptor subunit TOM9-1 OS=Arabidopsis thaliana GN=TOM9-1 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.600e-17
Identity = 49/91 (53.85%), Postives = 67/91 (73.63%), Query Frame = 1


		  
Query: 6  KRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFL 65
          K+IG+   +   +LA++    S+  I  +G ++A D   V+KKLLKSTGKAAWI GTTFL
Sbjct: 4  KKIGAGKGDSS-ILAKI----SNYDIVSQGRRAACDAVYVSKKLLKSTGKAAWIAGTTFL 63

Query: 66 VLALPLIIELERDGQLDAIELQQASLLGTPP 97
          +LA+PLI+ELE+D +L  I+ +QASLLGTPP
Sbjct: 64 ILAVPLILELEQDHRLGEIDFEQASLLGTPP 89

[Close]

BLAST of Spo26994.1 vs. TAIR (Arabidopsis)
Match: AT5G43970.1 (translocase of outer membrane 22-V)

HSP 1 Score: 98.2 bits (243), Expect = 3.000e-21
Identity = 53/93 (56.99%), Postives = 69/93 (74.19%), Query Frame = 1


		  
Query: 6  KRIGSSSDNGG--GVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTT 65
          KRIG+    GG   +LAR+    S+S I  +G ++A D   V+KKLL+STGKAAWI GTT
Sbjct: 4  KRIGAGKSGGGDPNILARI----SNSEIVSQGRRAAGDAVEVSKKLLRSTGKAAWIAGTT 63

Query: 66 FLVLALPLIIELERDGQLDAIELQQASLLGTPP 97
          FL+L +PLIIE++R+ Q++ IELQQASLLG PP
Sbjct: 64 FLILVVPLIIEMDREAQINEIELQQASLLGAPP 92

[Close]

BLAST of Spo26994.1 vs. TAIR (Arabidopsis)
Match: AT1G04070.1 (translocase of outer membrane 22-I)

HSP 1 Score: 88.2 bits (217), Expect = 3.100e-18
Identity = 49/91 (53.85%), Postives = 67/91 (73.63%), Query Frame = 1


		  
Query: 6  KRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFL 65
          K+IG+   +   +LA++    S+  I  +G ++A D   V+KKLLKSTGKAAWI GTTFL
Sbjct: 4  KKIGAGKGDSS-ILAKI----SNYDIVSQGRRAACDAVYVSKKLLKSTGKAAWIAGTTFL 63

Query: 66 VLALPLIIELERDGQLDAIELQQASLLGTPP 97
          +LA+PLI+ELE+D +L  I+ +QASLLGTPP
Sbjct: 64 ILAVPLILELEQDHRLGEIDFEQASLLGTPP 89

The following BLAST results are available for this feature:

BLAST of Spo26994.1 vs. NCBI nr
BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
BLAST of Spo26994.1 vs. ExPASy Swiss-Prot
BLAST of Spo26994.1 vs. TAIR (Arabidopsis)

BLAST of Spo26994.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902184558\|gb\|KNA10312.1\|	2.2e-45	100.	hypothetical protein SOVF_1454...	[more]
gi\|902183133\|gb\|KNA10063.1\|	1.2e-43	97.0	hypothetical protein SOVF_1479...	[more]
gi\|731372562\|ref\|XP_010666386.1\|	5.7e-38	90.8	PREDICTED: mitochondrial impor...	[more]
gi\|460389672\|ref\|XP_004240474.1\|	2.3e-23	62.3	PREDICTED: mitochondrial impor...	[more]
gi\|698513962\|ref\|XP_009801898.1\|	6.8e-23	66.6	PREDICTED: mitochondrial impor...	[more]

BLAST of Spo26994.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QSN9_SPIOL	1.5e-45	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QTM4_SPIOL	8.3e-44	97.0	Uncharacterized protein OS=Spi...	[more]
A0A0J8B4L3_BETVU	4.0e-38	90.8	Uncharacterized protein OS=Bet...	[more]
K4C3J2_SOLLC	1.6e-23	62.3	Uncharacterized protein OS=Sol...	[more]
A0A0V0GW57_SOLCH	8.1e-23	64.7	Putative mitochondrial import ...	[more]

BLAST of Spo26994.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2


Match Name	E-value	Identity	Description
TOM92_ARATH	5.4e-20	56.9	Mitochondrial import receptor ...	[more]
TOM91_ARATH	5.6e-17	53.8	Mitochondrial import receptor ...	[more]

BLAST of Spo26994.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G43970.1	3.0e-21	56.9	translocase of outer membrane ...	[more]
AT1G04070.1	3.1e-18	53.8	translocase of outer membrane ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005683	Mitochondrial import receptor subunit Tom22	PFAM	PF04281	Tom22	coord: 37..88 score: 5.
IPR017411	Mitochondrial outer membrane translocase complex, subunit Tom22, plant	PIR	PIRSF038151	TOM9-2_plant	coord: 1..100 score: 2.7
None	No IPR available	PANTHER	PTHR12504	MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22	coord: 37..98 score: 4.9

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0006626	protein targeting to mitochondrion
biological_process	GO:0071806	protein transmembrane transport
cellular_component	GO:0005741	mitochondrial outer membrane
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0005742	mitochondrial outer membrane translocase complex
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0015450	P-P-bond-hydrolysis-driven protein transmembrane transporter activity