Spo20378.1 (mRNA)

Overview
NameSpo20378.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionNADPH:protochlorophyllide oxidoreductase
LocationSpoScf_03747 : 93 .. 350 (-)
Sequence length150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTACAAGCAGCTTCTTTGGTACCTTCTTCAGTCTCATTTCATAAAGAGGTATGTTTATGATAATCTTGCAATTTTATCATTAAATTAATGTTAATTTTGGTTATCTGTTTAATTTTTTTTATGAAATGTTAAAAGTTTTGGTTGGAATGATATCAGGGGAAATGCATTGGTTTTAAAGAGACATCCTTTTTTGGGGTTTCATTTTCTGATCATTTGAAGGCTGAATTTCATGCTCCATCTTTGAGAATCAAG

mRNA sequence

ATGGCGCTACAAGCAGCTTCTTTGGTACCTTCTTCAGTCTCATTTCATAAAGAGGGGAAATGCATTGGTTTTAAAGAGACATCCTTTTTTGGGGTTTCATTTTCTGATCATTTGAAGGCTGAATTTCATGCTCCATCTTTGAGAATCAAG

Coding sequence (CDS)

ATGGCGCTACAAGCAGCTTCTTTGGTACCTTCTTCAGTCTCATTTCATAAAGAGGGGAAATGCATTGGTTTTAAAGAGACATCCTTTTTTGGGGTTTCATTTTCTGATCATTTGAAGGCTGAATTTCATGCTCCATCTTTGAGAATCAAG

Protein sequence

MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo20378Spo20378gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo20378.1Spo20378.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20378.1.CDS.2Spo20378.1.CDS.2CDS
Spo20378.1.CDS.1Spo20378.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20378.1.exon.2Spo20378.1.exon.2exon
Spo20378.1.exon.1Spo20378.1.exon.1exon


Homology
[Close]
BLAST of Spo20378.1 vs. NCBI nr
Match: gi|902169173|gb|KNA07626.1| (hypothetical protein SOVF_170120 isoform A [Spinacia oleracea])

HSP 1 Score: 103.2 bits (256), Expect = 1.300e-19
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK
Sbjct: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 50
[Close]
BLAST of Spo20378.1 vs. NCBI nr
Match: gi|902169174|gb|KNA07627.1| (hypothetical protein SOVF_170120 isoform B [Spinacia oleracea])

HSP 1 Score: 103.2 bits (256), Expect = 1.300e-19
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK
Sbjct: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 50
[Close]
BLAST of Spo20378.1 vs. NCBI nr
Match: gi|731356449|ref|XP_010689680.1| (PREDICTED: protochlorophyllide reductase-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 86.3 bits (212), Expect = 1.700e-14
Identity = 41/50 (82.00%), Postives = 45/50 (90.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLVPSSV+FHKEGK IGFKETSF G+S S+H KAEFH P+LRIK
Sbjct: 1  MALQAASLVPSSVAFHKEGKHIGFKETSFLGISISEHFKAEFHPPTLRIK 50
[Close]
BLAST of Spo20378.1 vs. NCBI nr
Match: gi|702364656|ref|XP_010060532.1| (PREDICTED: protochlorophyllide reductase, chloroplastic [Eucalyptus grandis])

HSP 1 Score: 60.1 bits (144), Expect = 1.300e-6
Identity = 30/51 (58.82%), Postives = 41/51 (80.39%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC-IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAA+LVPS+ +  KEGK    FK++S FGVSFS+H+KA+F++ +LR K
Sbjct: 1  MALQAAALVPSAFTIPKEGKSSASFKDSSLFGVSFSEHVKADFNSSALRCK 51
[Close]
BLAST of Spo20378.1 vs. NCBI nr
Match: gi|823222044|ref|XP_012443722.1| (PREDICTED: protochlorophyllide reductase, chloroplastic [Gossypium raimondii])

HSP 1 Score: 59.3 bits (142), Expect = 2.200e-6
Identity = 31/51 (60.78%), Postives = 39/51 (76.47%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC-IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAA+LVPS+ S  KEGK    FK++S FGVS SDH+KA+F + +LR K
Sbjct: 1  MALQAAALVPSTFSVPKEGKSGASFKDSSLFGVSLSDHIKADFTSSALRCK 51
[Close]
BLAST of Spo20378.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QK60_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_170120 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 9.300e-20
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK
Sbjct: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 50
[Close]
BLAST of Spo20378.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QM45_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_170120 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 9.300e-20
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK
Sbjct: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 50
[Close]
BLAST of Spo20378.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EG80_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g208630 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.200e-14
Identity = 41/50 (82.00%), Postives = 45/50 (90.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLVPSSV+FHKEGK IGFKETSF G+S S+H KAEFH P+LRIK
Sbjct: 1  MALQAASLVPSSVAFHKEGKHIGFKETSFLGISISEHFKAEFHPPTLRIK 50
[Close]
BLAST of Spo20378.1 vs. UniProtKB/TrEMBL
Match: G5DVX7_SILLA (Protochlorophyllide reductase B (Fragment) OS=Silene latifolia PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.000e-11
Identity = 38/50 (76.00%), Postives = 39/50 (78.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          M+LQAA LVPSSV  HKEGK   FKETSF GV  SDHLKAE H PSLRIK
Sbjct: 1  MSLQAACLVPSSVLLHKEGKYGSFKETSFLGVPMSDHLKAEAHFPSLRIK 50
[Close]
BLAST of Spo20378.1 vs. UniProtKB/TrEMBL
Match: G5DVX6_SILLA (Protochlorophyllide reductase B (Fragment) OS=Silene latifolia PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.300e-10
Identity = 38/50 (76.00%), Postives = 39/50 (78.00%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKCIGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          M+LQAA LVPSSVS  KEGK   FKETSF GV  SDHLKAE H PSLRIK
Sbjct: 1  MSLQAACLVPSSVSLLKEGKYGSFKETSFLGVPMSDHLKAEAHFPSLRIK 50
[Close]
BLAST of Spo20378.1 vs. ExPASy Swiss-Prot
Match: PORA_CUCSA (Protochlorophyllide reductase, chloroplastic OS=Cucumis sativus GN=PORA PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.000e-8
Identity = 30/51 (58.82%), Postives = 39/51 (76.47%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC-IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLV  ++S  KEGK  +  K++S FG+SFSDHLK+EF + +LR K
Sbjct: 1  MALQAASLVSPALSIPKEGKSSVCLKDSSLFGISFSDHLKSEFSSSTLRCK 51
[Close]
BLAST of Spo20378.1 vs. ExPASy Swiss-Prot
Match: POR_DAUCA (Protochlorophyllide reductase, chloroplastic OS=Daucus carota GN=POR1 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 7.600e-7
Identity = 27/43 (62.79%), Postives = 33/43 (76.74%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC-IGFKETSFFGVSFSDHLKAEF 43
          MALQAAS +PSS S +KEGK  +  KETS FGV+FSD L+ +F
Sbjct: 1  MALQAASFLPSSFSINKEGKANVSLKETSLFGVTFSDSLRTDF 43
[Close]
BLAST of Spo20378.1 vs. ExPASy Swiss-Prot
Match: PORA_ARATH (Protochlorophyllide reductase A, chloroplastic OS=Arabidopsis thaliana GN=PORA PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 4.900e-6
Identity = 30/55 (54.55%), Postives = 36/55 (65.45%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC-----IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLV S+ S  K+GK        FKE+S FGVS S+  KA+F + SLR K
Sbjct: 1  MALQAASLVSSAFSVRKDGKLNASASSSFKESSLFGVSLSEQSKADFVSSSLRCK 55
[Close]
BLAST of Spo20378.1 vs. TAIR (Arabidopsis)
Match: AT5G54190.1 (protochlorophyllide oxidoreductase A)

HSP 1 Score: 50.8 bits (120), Expect = 2.800e-7
Identity = 30/55 (54.55%), Postives = 36/55 (65.45%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC-----IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLV S+ S  K+GK        FKE+S FGVS S+  KA+F + SLR K
Sbjct: 1  MALQAASLVSSAFSVRKDGKLNASASSSFKESSLFGVSLSEQSKADFVSSSLRCK 55
[Close]
BLAST of Spo20378.1 vs. TAIR (Arabidopsis)
Match: AT1G03630.1 (protochlorophyllide oxidoreductase C)

HSP 1 Score: 48.9 bits (115), Expect = 1.100e-6
Identity = 30/52 (57.69%), Postives = 37/52 (71.15%), Query Frame = 1 

		  

Query: 1  MALQAA-SLVPSSVSFHKEGKC-IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAA SL+PS++S  KEGK     KET+F G SFS+HL+AE  +  L IK
Sbjct: 1  MALQAAYSLLPSTISIQKEGKFNASLKETTFTGSSFSNHLRAEKISTLLTIK 52
[Close]
BLAST of Spo20378.1 vs. TAIR (Arabidopsis)
Match: AT4G27440.1 (protochlorophyllide oxidoreductase B)

HSP 1 Score: 46.2 bits (108), Expect = 6.800e-6
Identity = 26/54 (48.15%), Postives = 34/54 (62.96%), Query Frame = 1 

		  

Query: 1  MALQAASLVPSSVSFHKEGKC----IGFKETSFFGVSFSDHLKAEFHAPSLRIK 51
          MALQAASLV S+ S  K+ K       FK++S FG S +D +K+E  + SLR K
Sbjct: 1  MALQAASLVSSAFSVRKDAKLNASSSSFKDSSLFGASITDQIKSEHGSSSLRFK 54
The following BLAST results are available for this feature:
BLAST of Spo20378.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902169173|gb|KNA07626.1|1.3e-19100.hypothetical protein SOVF_1701... [more]
gi|902169174|gb|KNA07627.1|1.3e-19100.hypothetical protein SOVF_1701... [more]
gi|731356449|ref|XP_010689680.1|1.7e-1482.0PREDICTED: protochlorophyllide... [more]
gi|702364656|ref|XP_010060532.1|1.3e-658.8PREDICTED: protochlorophyllide... [more]
gi|823222044|ref|XP_012443722.1|2.2e-660.7PREDICTED: protochlorophyllide... [more]
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BLAST of Spo20378.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QK60_SPIOL9.3e-20100.Uncharacterized protein OS=Spi... [more]
A0A0K9QM45_SPIOL9.3e-20100.Uncharacterized protein OS=Spi... [more]
A0A0J8EG80_BETVU1.2e-1482.0Uncharacterized protein OS=Bet... [more]
G5DVX7_SILLA6.0e-1176.0Protochlorophyllide reductase ... [more]
G5DVX6_SILLA2.3e-1076.0Protochlorophyllide reductase ... [more]
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BLAST of Spo20378.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 3
Match NameE-valueIdentityDescription
PORA_CUCSA4.0e-858.8Protochlorophyllide reductase,... [more]
POR_DAUCA7.6e-762.7Protochlorophyllide reductase,... [more]
PORA_ARATH4.9e-654.5Protochlorophyllide reductase ... [more]
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BLAST of Spo20378.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3
Match NameE-valueIdentityDescription
AT5G54190.12.8e-754.5protochlorophyllide oxidoreduc... [more]
AT1G03630.11.1e-657.6protochlorophyllide oxidoreduc... [more]
AT4G27440.16.8e-648.1protochlorophyllide oxidoreduc... [more]
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GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0019685 photosynthesis, dark reaction
cellular_component GO:0009536 plastid
molecular_function GO:0016630 protochlorophyllide reductase activity