Spo16424.1 (mRNA) | SpinachBase

Overview


Name	Spo16424.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	2-oxoglutarate/malate translocator, chloroplastic
Location	SpoScf_28364 : 139 .. 969 (-)
Sequence length	831

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATACTATATGCCAAGAACATTCTTCCGTCGATAACAACAACGATCCAAATACAAATTCCCTCAACACCCCTATCCTCCCAACCCGAACAGCCTCCCGGTTCAGAACCCGGTTTCCAAATTTCCACTGGGACGGAGTTAAACCAATCCCATTCGTAATATCTATCACCACCGGTCTAATTATCCGGTTCGCAATCCCTAAACCGGACAACGTCTCACCCCAAGCATGGACTCTTCTATCCATTTTCATGAGCACAATATCCGGTCTAGTTTTCGCTCCTTTACCGGTGGGAGCATGGTCTTTCGTGTGCTTAACAGCAACCTTGATCACGAATACGCTGTCGTTTAAAGAGGCATTCAGTGCGATGACAAATGAAGTGATATGGTTAATAGTAGTGGCTTTCTTCTTCTCACGTGGGTTCGTGAAAACCGGTCTAGGAGACCGGGTTGCGACGTGTTTTATAAAGTGGATGGGAAGGAGAACAGTCGGTTTGGCTTACGGTTTGGTGCTGAGTGAGGCATTGATTTGCCCCGCGATACCGAGCTCGACGGCTCGAGCCGGTGGAGTGTTTAGCCCGGTGATAAAATCGGTTGCGGTGAATAATGGGAGTATGCCGTTTGATAACAAGTCGGGTAAGAAGATTGGATCTTATCTAGTTATGGCACAATTGCAGGTATGTGTATATGTAGTTGTTACTTTCCTTGTTCTATCACTAGTAGAGTGTATTGGAAATGAGTGTCATAGTGCAAAGACCACGTATGTGACCTTATTTGATGAGGTGGAAATGTTTTGGATAAGGGATGTTTTCATGTTTGAGATTGTGGTATAG

mRNA sequence

ATGGATACTATATGCCAAGAACATTCTTCCGTCGATAACAACAACGATCCAAATACAAATTCCCTCAACACCCCTATCCTCCCAACCCGAACAGCCTCCCGGTTCAGAACCCGGTTTCCAAATTTCCACTGGGACGGAGTTAAACCAATCCCATTCGTAATATCTATCACCACCGGTCTAATTATCCGGTTCGCAATCCCTAAACCGGACAACGTCTCACCCCAAGCATGGACTCTTCTATCCATTTTCATGAGCACAATATCCGGTCTAGTTTTCGCTCCTTTACCGGTGGGAGCATGGTCTTTCGTGTGCTTAACAGCAACCTTGATCACGAATACGCTGTCGTTTAAAGAGGCATTCAGTGCGATGACAAATGAAGTGATATGGTTAATAGTAGTGGCTTTCTTCTTCTCACGTGGGTTCGTGAAAACCGGTCTAGGAGACCGGGTTGCGACGTGTTTTATAAAGTGGATGGGAAGGAGAACAGTCGGTTTGGCTTACGGTTTGGTGCTGAGTGAGGCATTGATTTGCCCCGCGATACCGAGCTCGACGGCTCGAGCCGGTGGAGTGTTTAGCCCGGTGATAAAATCGGTTGCGGTGAATAATGGGAGTATGCCGTTTGATAACAAGTCGGGTAAGAAGATTGGATCTTATCTAGTTATGGCACAATTGCAGGTATGTGTATATGTAGTTGTTACTTTCCTTGTTCTATCACTAGTAGAGTGTATTGGAAATGAGTGTCATAGTGCAAAGACCACGTATGTGACCTTATTTGATGAGGTGGAAATGTTTTGGATAAGGGATGTTTTCATGTTTGAGATTGTGGTATAG

Coding sequence (CDS)

ATGGATACTATATGCCAAGAACATTCTTCCGTCGATAACAACAACGATCCAAATACAAATTCCCTCAACACCCCTATCCTCCCAACCCGAACAGCCTCCCGGTTCAGAACCCGGTTTCCAAATTTCCACTGGGACGGAGTTAAACCAATCCCATTCGTAATATCTATCACCACCGGTCTAATTATCCGGTTCGCAATCCCTAAACCGGACAACGTCTCACCCCAAGCATGGACTCTTCTATCCATTTTCATGAGCACAATATCCGGTCTAGTTTTCGCTCCTTTACCGGTGGGAGCATGGTCTTTCGTGTGCTTAACAGCAACCTTGATCACGAATACGCTGTCGTTTAAAGAGGCATTCAGTGCGATGACAAATGAAGTGATATGGTTAATAGTAGTGGCTTTCTTCTTCTCACGTGGGTTCGTGAAAACCGGTCTAGGAGACCGGGTTGCGACGTGTTTTATAAAGTGGATGGGAAGGAGAACAGTCGGTTTGGCTTACGGTTTGGTGCTGAGTGAGGCATTGATTTGCCCCGCGATACCGAGCTCGACGGCTCGAGCCGGTGGAGTGTTTAGCCCGGTGATAAAATCGGTTGCGGTGAATAATGGGAGTATGCCGTTTGATAACAAGTCGGGTAAGAAGATTGGATCTTATCTAGTTATGGCACAATTGCAGGTATGTGTATATGTAGTTGTTACTTTCCTTGTTCTATCACTAGTAGAGTGTATTGGAAATGAGTGTCATAGTGCAAAGACCACGTATGTGACCTTATTTGATGAGGTGGAAATGTTTTGGATAAGGGATGTTTTCATGTTTGAGATTGTGGTATAG

Protein sequence

MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSLVECIGNECHSAKTTYVTLFDEVEMFWIRDVFMFEIVV

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo16424	Spo16424	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo16424.1	Spo16424.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16424.1.CDS.1	Spo16424.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16424.1.exon.1	Spo16424.1.exon.1	exon

Homology

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BLAST of Spo16424.1 vs. NCBI nr
Match: gi|902233151|gb|KNA22944.1| (hypothetical protein SOVF_029340 [Spinacia oleracea])

HSP 1 Score: 478.4 bits (1230), Expect = 8.400e-132
Identity = 242/243 (99.59%), Postives = 243/243 (100.00%), Query Frame = 1


		  
Query: 1   MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGL 60
           MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGL
Sbjct: 1   MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGL 60

Query: 61  IIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAF 120
           IIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAF
Sbjct: 61  IIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAF 120

Query: 121 SAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAI 180
           SAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAI
Sbjct: 121 SAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAI 180

Query: 181 PSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSLV 240
           PSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLS+V
Sbjct: 181 PSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSVV 240

Query: 241 ECI 244
           ECI
Sbjct: 241 ECI 243

[Close]

BLAST of Spo16424.1 vs. NCBI nr
Match: gi|870849289|gb|KMT01552.1| (hypothetical protein BVRB_9g215530 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 317.8 bits (813), Expect = 1.900e-83
Identity = 165/237 (69.62%), Postives = 192/237 (81.01%), Query Frame = 1


		  
Query: 7   EHSSVDNNNDPNTNSLNTPILPTRTA----SRFRTRFPNFHWDGVKPIPFVISITTGLII 66
           EH     +   + NS+NTPILPTRT     SR +TRF N HW GVKPIP + +  TGL+I
Sbjct: 12  EHGHEHEHIITDPNSINTPILPTRTTTTTTSRVKTRFTNLHWHGVKPIPLLFATITGLLI 71

Query: 67  RFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAFSA 126
           RFA+PKP  VS +AWTLLSIF++T++GLV  PLPVGAW+FVCLT +LIT TLSF+E FSA
Sbjct: 72  RFAVPKPAKVSTRAWTLLSIFVTTVAGLVLDPLPVGAWAFVCLTLSLITKTLSFEEGFSA 131

Query: 127 MTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAIPS 186
           MTNEVIWLIVVAFFFSRGFVKTGLGDR+A  FIKW+GR+TVGL+YGLVLSEALI  AIPS
Sbjct: 132 MTNEVIWLIVVAFFFSRGFVKTGLGDRLAMYFIKWLGRKTVGLSYGLVLSEALISAAIPS 191

Query: 187 STARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSL 240
           +TARAGGVFSPVIKSVAV+NGS+ FD KS KKIG+YL M+QLQV + +V     LSL
Sbjct: 192 TTARAGGVFSPVIKSVAVSNGSVAFDKKSAKKIGAYLAMSQLQVSINIVFCTFSLSL 248

[Close]

BLAST of Spo16424.1 vs. NCBI nr
Match: gi|698569022|ref|XP_009774220.1| (PREDICTED: dicarboxylate transporter 2.1, chloroplastic-like [Nicotiana sylvestris])

HSP 1 Score: 257.3 bits (656), Expect = 3.100e-65
Identity = 124/198 (62.63%), Postives = 155/198 (78.28%), Query Frame = 1


		  
Query: 28  PTRTASRFRTRFPNFHWDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTI 87
           PT  A+R R R     W G KPIP  ISI  G++ RFA+PKP  +S +AW LL+IF++TI
Sbjct: 11  PTSNANRHRRRISLPPWKGAKPIPLAISILIGILFRFAVPKPHQLSTKAWQLLAIFLTTI 70

Query: 88  SGLVFAPLPVGAWSFVCLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLG 147
           SGL+  PLPVGAW+F+CLT T++T TL+F  AF+A TNEVIWLIV +FFFSRGF+KTGLG
Sbjct: 71  SGLILGPLPVGAWAFLCLTLTVVTKTLTFAAAFAAFTNEVIWLIVASFFFSRGFIKTGLG 130

Query: 148 DRVATCFIKWMGRRTVGLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPF 207
           DRVA CF+ W+G+ T+GL+YGL LSEA I PAIPS+TARAGG+F P+IKS+AV   S P 
Sbjct: 131 DRVALCFVSWLGKNTLGLSYGLALSEATISPAIPSTTARAGGIFLPIIKSLAVTADSHPK 190

Query: 208 DNKSGKKIGSYLVMAQLQ 226
           D+ S KK+G+YLV +QLQ
Sbjct: 191 DD-SAKKLGAYLVQSQLQ 207

[Close]

BLAST of Spo16424.1 vs. NCBI nr
Match: gi|697135458|ref|XP_009621784.1| (PREDICTED: dicarboxylate transporter 2.1, chloroplastic-like [Nicotiana tomentosiformis])

HSP 1 Score: 255.8 bits (652), Expect = 8.900e-65
Identity = 124/198 (62.63%), Postives = 154/198 (77.78%), Query Frame = 1


		  
Query: 28  PTRTASRFRTRFPNFHWDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTI 87
           PT   SR R R     W G KPIP  ISI  G++ RFA+PKP  +S +AW LL+IF++TI
Sbjct: 14  PTSNTSRHRRRISLPTWKGAKPIPLAISILIGILFRFAVPKPHQLSTKAWQLLAIFLTTI 73

Query: 88  SGLVFAPLPVGAWSFVCLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLG 147
           SGL+  PLPVGAW+F+CLT T++T TL+F  AF+A TNEVIWLIV +FFFSRGF+KTGLG
Sbjct: 74  SGLILGPLPVGAWAFLCLTLTVVTKTLTFAAAFAAFTNEVIWLIVASFFFSRGFIKTGLG 133

Query: 148 DRVATCFIKWMGRRTVGLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPF 207
           DRVA CF+ W+G+ T+GL+YGL LSEA I PAIPS+TARAGG+F P+IKS+AV   S P 
Sbjct: 134 DRVALCFVCWLGKNTLGLSYGLALSEAAISPAIPSTTARAGGIFLPIIKSLAVTADSHPK 193

Query: 208 DNKSGKKIGSYLVMAQLQ 226
           D+ S KK+G+YLV +QLQ
Sbjct: 194 DD-SAKKLGAYLVQSQLQ 210

[Close]

BLAST of Spo16424.1 vs. NCBI nr
Match: gi|658022225|ref|XP_008346519.1| (PREDICTED: dicarboxylate transporter 2, chloroplastic-like [Malus domestica])

HSP 1 Score: 252.7 bits (644), Expect = 7.500e-64
Identity = 119/182 (65.38%), Postives = 156/182 (85.71%), Query Frame = 1


		  
Query: 45  DGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVC 104
           +G KPIPF+ISI  G+++RF +PKP  V+PQAW LL+IF+STI+GLV +PLPVGAW+F+ 
Sbjct: 93  EGAKPIPFIISIAIGIVVRFFVPKPVEVTPQAWQLLAIFLSTIAGLVLSPLPVGAWAFLG 152

Query: 105 LTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVG 164
           LT +++T TLSF  AFSA TNEVIWLIV++FFF+RGFVKTGLGDR+AT F+KW+G+ T+G
Sbjct: 153 LTTSVVTGTLSFPTAFSAFTNEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLG 212

Query: 165 LAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQL 224
           L+YGL +SEALI PA+PS+TARAGGVF P+IKS++++ GS P D  S +K+GSYL+M+Q 
Sbjct: 213 LSYGLTISEALIAPAMPSTTARAGGVFLPIIKSLSLSGGSKPGD-PSKRKLGSYLIMSQF 272

Query: 225 QV 227
           QV
Sbjct: 273 QV 273

[Close]

BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RVK3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_029340 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 5.900e-132
Identity = 242/243 (99.59%), Postives = 243/243 (100.00%), Query Frame = 1


		  
Query: 1   MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGL 60
           MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGL
Sbjct: 1   MDTICQEHSSVDNNNDPNTNSLNTPILPTRTASRFRTRFPNFHWDGVKPIPFVISITTGL 60

Query: 61  IIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAF 120
           IIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAF
Sbjct: 61  IIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAF 120

Query: 121 SAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAI 180
           SAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAI
Sbjct: 121 SAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAI 180

Query: 181 PSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSLV 240
           PSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLS+V
Sbjct: 181 PSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSVV 240

Query: 241 ECI 244
           ECI
Sbjct: 241 ECI 243

[Close]

BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BKM9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g215530 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.300e-83
Identity = 165/237 (69.62%), Postives = 192/237 (81.01%), Query Frame = 1


		  
Query: 7   EHSSVDNNNDPNTNSLNTPILPTRTA----SRFRTRFPNFHWDGVKPIPFVISITTGLII 66
           EH     +   + NS+NTPILPTRT     SR +TRF N HW GVKPIP + +  TGL+I
Sbjct: 12  EHGHEHEHIITDPNSINTPILPTRTTTTTTSRVKTRFTNLHWHGVKPIPLLFATITGLLI 71

Query: 67  RFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCLTATLITNTLSFKEAFSA 126
           RFA+PKP  VS +AWTLLSIF++T++GLV  PLPVGAW+FVCLT +LIT TLSF+E FSA
Sbjct: 72  RFAVPKPAKVSTRAWTLLSIFVTTVAGLVLDPLPVGAWAFVCLTLSLITKTLSFEEGFSA 131

Query: 127 MTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGLAYGLVLSEALICPAIPS 186
           MTNEVIWLIVVAFFFSRGFVKTGLGDR+A  FIKW+GR+TVGL+YGLVLSEALI  AIPS
Sbjct: 132 MTNEVIWLIVVAFFFSRGFVKTGLGDRLAMYFIKWLGRKTVGLSYGLVLSEALISAAIPS 191

Query: 187 STARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQVCVYVVVTFLVLSL 240
           +TARAGGVFSPVIKSVAV+NGS+ FD KS KKIG+YL M+QLQV + +V     LSL
Sbjct: 192 TTARAGGVFSPVIKSVAVSNGSVAFDKKSAKKIGAYLAMSQLQVSINIVFCTFSLSL 248

[Close]

BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
Match: A0A0C9S8S9_9SPER (TSA: Wollemia nobilis Ref_Wollemi_Transcript_3696_2163 transcribed RNA sequence OS=Wollemia nobilis PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.600e-66
Identity = 122/184 (66.30%), Postives = 154/184 (83.70%), Query Frame = 1


		  
Query: 44  WDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFV 103
           W G KP+P VIS+  GLI+RF +PKP  V+ QAW LLSIF+STI+GLV +PLPVGAW+F+
Sbjct: 101 WQGAKPLPLVISVAVGLIVRFLVPKPVEVTAQAWQLLSIFLSTIAGLVLSPLPVGAWAFI 160

Query: 104 CLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTV 163
            LT  +IT TLSF  AFSA TNEVIWLIV++FFF+RGFVKTGLGDR+AT F+KWMG+ T+
Sbjct: 161 GLTTAVITRTLSFTAAFSAFTNEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTL 220

Query: 164 GLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQ 223
           GL+YGL +SEALI PA+PS+TARAGGVF P+IKS++++ GS+P DN S KK+G+YL+ +Q
Sbjct: 221 GLSYGLTVSEALIAPAMPSTTARAGGVFLPIIKSLSLSRGSLPNDN-SSKKLGAYLIQSQ 280

Query: 224 LQVC 228
            Q C
Sbjct: 281 FQAC 283

[Close]

BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
Match: M5VJG8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003588mg PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.000e-63
Identity = 119/180 (66.11%), Postives = 154/180 (85.56%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G KPIPF+ISI  GL++RF +PKP  VSPQAW LL+IF+STI+GLV +PLPVGAW+F+ L
Sbjct: 93  GAKPIPFIISIAIGLVVRFLVPKPVEVSPQAWQLLAIFLSTIAGLVLSPLPVGAWAFLGL 152

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           T++++T TL F  AFSA TNEVIWLIV++FFF+RGFVKTGLGDR+AT F+KW+G+ T+GL
Sbjct: 153 TSSIVTGTLPFTTAFSAFTNEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGL 212

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL +SEALI PA+PS+TARAGGVF P+IKS++++ GS P D+ S  K+GSYL+M+Q Q
Sbjct: 213 SYGLTISEALIAPAMPSTTARAGGVFLPIIKSLSLSAGSKP-DHPSKGKLGSYLIMSQFQ 271

[Close]

BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
Match: M1AXS7_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400012543 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.800e-63
Identity = 118/182 (64.84%), Postives = 154/182 (84.62%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G K IP +IS++ GLI+RF +P+P  VSPQAW LLSIF+STI+GLV +PLPVGAW+F+ L
Sbjct: 114 GAKLIPLIISVSIGLIVRFLVPRPPEVSPQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGL 173

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           T +++T TL+F  AFSA TNEVIWLIV++FFF+RGFVKTGLGDR+AT F+KW+G+ T+GL
Sbjct: 174 TTSVLTKTLTFSSAFSAFTNEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGL 233

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL L+EALI PA+PS+TARAGGVF P+IKS++++ GS P D  S +K+GSYL+ +Q Q
Sbjct: 234 SYGLTLAEALIAPAMPSTTARAGGVFLPIIKSLSLSAGSKPGD-PSSRKLGSYLIQSQFQ 293

Query: 226 VC 228
           VC
Sbjct: 294 VC 294

[Close]

BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
Match: DIT21_ARATH (Dicarboxylate transporter 2.1, chloroplastic OS=Arabidopsis thaliana GN=DIT2-1 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.400e-64
Identity = 116/180 (64.44%), Postives = 154/180 (85.56%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G K IP ++SI+ GLI+RFA+P P+ V+PQ W LLSIF+STI+GLV +PLPVGAW+F+ L
Sbjct: 93  GAKLIPLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVLSPLPVGAWAFIGL 152

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           TA+++T TLSF  AFSA T+EVIWLIV++FFF+RGFVKTGLGDR+AT F+KW+G+ T+GL
Sbjct: 153 TASIVTKTLSFSAAFSAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGL 212

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL LSEALI PA+PS+TARAGG+F P+IKS++++ GS P D+ S +K+GSYL+ +Q Q
Sbjct: 213 SYGLTLSEALIAPAMPSTTARAGGIFLPIIKSLSLSAGSKPNDS-SSRKLGSYLIQSQFQ 271

[Close]

BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
Match: DIT2_SPIOL (Dicarboxylate transporter 2, chloroplastic OS=Spinacia oleracea GN=DIT2 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 8.300e-63
Identity = 109/180 (60.56%), Postives = 154/180 (85.56%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G K IP ++S++ GL++RFA+PKP  ++PQAW LL+IF+ST++GLV +PLPVGAW+F+ +
Sbjct: 94  GAKLIPLILSVSVGLLLRFAVPKPAELTPQAWQLLAIFLSTVAGLVLSPLPVGAWAFLGV 153

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           TA+++T TL F  AF A TNEVIWLIV++FFF+RGFVKTGLGDR+AT F+KW+G+ T+GL
Sbjct: 154 TASVVTKTLPFPTAFCAFTNEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGL 213

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL +SEAL+ PA+PS+TARAGG+F P+IKS+++++GS+P   +S KK+G+YL+M Q Q
Sbjct: 214 SYGLTISEALVAPAMPSTTARAGGIFLPIIKSLSISSGSLP-GGESRKKLGTYLIMTQFQ 272

[Close]

BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
Match: DIT22_ARATH (Dicarboxylate transporter 2.2, chloroplastic OS=Arabidopsis thaliana GN=DIT2-2 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.000e-57
Identity = 107/180 (59.44%), Postives = 141/180 (78.33%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G K IP  IS++ GLI+RF IP+P+ V+ Q W LLSIF+ TISGLV  PLPVGAW+F+ L
Sbjct: 79  GAKLIPLAISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTISGLVLGPLPVGAWAFIGL 138

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           TA+++T TL F  AF+A TNE+IWLI ++FFF+RGF+KTGLGDR+AT F+KW+G+ T+GL
Sbjct: 139 TASIVTKTLPFSTAFAAFTNELIWLIAISFFFARGFIKTGLGDRIATYFVKWLGKSTLGL 198

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL   E L+   +PS+ ARAGGVF PVIKS+A++ GS P D  S +K+GS+L+  QLQ
Sbjct: 199 SYGLAFCETLMGLIMPSTMARAGGVFLPVIKSLAISAGSYPGD-PSSRKLGSFLIQTQLQ 257

[Close]

BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
Match: DIT1_SPIOL (Dicarboxylate transporter 1, chloroplastic OS=Spinacia oleracea GN=DIT1 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 6.800e-41
Identity = 100/229 (43.67%), Postives = 144/229 (62.88%), Query Frame = 1


		  
Query: 44  WDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFV 103
           W G    P + SI TG+II F IP P+ VS  AW LL+IF+STI G++  PLP+GA + +
Sbjct: 99  WQGASIKPLLASILTGVIIWF-IPTPEGVSRNAWQLLAIFLSTIVGIITQPLPLGAVALM 158

Query: 104 CLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTV 163
            L A+++T TL+F  AFSA  + + WLI +AFFF+RGF+KTGLG+R+A  F+K  G  ++
Sbjct: 159 GLGASVLTKTLTFSAAFSAFGDPIPWLIALAFFFARGFIKTGLGNRIAYQFVKLFGSSSL 218

Query: 164 GLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQ 223
           GL Y LV SEAL+ PAIPS +ARAGG+F P++KS+ +  GS   D  + +K+G++L++  
Sbjct: 219 GLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCIACGSNVGDG-TERKLGAWLMLTC 278

Query: 224 LQVCVYVVVTFLVLSLVECIGNECHSAKTTYVTLFDEVE-MFWIRDVFM 272
            Q  V     FL       +     SA  T+ T+   +  M W +  F+
Sbjct: 279 FQTSVISSSMFLTAMAANPL-----SATLTFNTIGKAIGWMDWAKAAFV 320

[Close]

BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
Match: DIT1_ARATH (Dicarboxylate transporter 1, chloroplastic OS=Arabidopsis thaliana GN=DIT1 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.400e-40
Identity = 91/192 (47.40%), Postives = 130/192 (67.71%), Query Frame = 1


		  
Query: 44  WDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFV 103
           W G    P + SI TGLI+ F +P P+ V+  AW LL+IF++TI G++  PLP+GA + +
Sbjct: 87  WQGAAIKPLLASIATGLILWF-VPVPEGVTRNAWQLLAIFLATIVGIITQPLPLGAVALM 146

Query: 104 CLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTV 163
            L A+++T TL+F  AFSA  + + WLI +AFFF+RGF+KTGLG+RVA  F++  G  ++
Sbjct: 147 GLGASVLTKTLTFAAAFSAFGDPIPWLIALAFFFARGFIKTGLGNRVAYQFVRLFGSSSL 206

Query: 164 GLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQ 223
           GL Y LV SEAL+ PAIPS +ARAGG+F P++KS+ V  GS   D  +  ++GS+L++  
Sbjct: 207 GLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCVACGSNVGDG-TEHRLGSWLMLTC 266

Query: 224 LQVCVYVVVTFL 236
            Q  V     FL
Sbjct: 267 FQTSVISSSMFL 276

[Close]

BLAST of Spo16424.1 vs. TAIR (Arabidopsis)
Match: AT5G64290.1 (dicarboxylate transport 2.1)

HSP 1 Score: 246.5 bits (628), Expect = 1.900e-65
Identity = 116/180 (64.44%), Postives = 154/180 (85.56%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G K IP ++SI+ GLI+RFA+P P+ V+PQ W LLSIF+STI+GLV +PLPVGAW+F+ L
Sbjct: 93  GAKLIPLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVLSPLPVGAWAFIGL 152

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           TA+++T TLSF  AFSA T+EVIWLIV++FFF+RGFVKTGLGDR+AT F+KW+G+ T+GL
Sbjct: 153 TASIVTKTLSFSAAFSAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGL 212

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL LSEALI PA+PS+TARAGG+F P+IKS++++ GS P D+ S +K+GSYL+ +Q Q
Sbjct: 213 SYGLTLSEALIAPAMPSTTARAGGIFLPIIKSLSLSAGSKPNDS-SSRKLGSYLIQSQFQ 271

[Close]

BLAST of Spo16424.1 vs. TAIR (Arabidopsis)
Match: AT5G64280.1 (dicarboxylate transporter 2.2)

HSP 1 Score: 223.0 bits (567), Expect = 2.200e-58
Identity = 107/180 (59.44%), Postives = 141/180 (78.33%), Query Frame = 1


		  
Query: 46  GVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFVCL 105
           G K IP  IS++ GLI+RF IP+P+ V+ Q W LLSIF+ TISGLV  PLPVGAW+F+ L
Sbjct: 79  GAKLIPLAISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTISGLVLGPLPVGAWAFIGL 138

Query: 106 TATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTVGL 165
           TA+++T TL F  AF+A TNE+IWLI ++FFF+RGF+KTGLGDR+AT F+KW+G+ T+GL
Sbjct: 139 TASIVTKTLPFSTAFAAFTNELIWLIAISFFFARGFIKTGLGDRIATYFVKWLGKSTLGL 198

Query: 166 AYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQLQ 225
           +YGL   E L+   +PS+ ARAGGVF PVIKS+A++ GS P D  S +K+GS+L+  QLQ
Sbjct: 199 SYGLAFCETLMGLIMPSTMARAGGVFLPVIKSLAISAGSYPGD-PSSRKLGSFLIQTQLQ 257

[Close]

BLAST of Spo16424.1 vs. TAIR (Arabidopsis)
Match: AT5G12860.1 (dicarboxylate transporter 1)

HSP 1 Score: 166.8 bits (421), Expect = 1.900e-41
Identity = 91/192 (47.40%), Postives = 130/192 (67.71%), Query Frame = 1


		  
Query: 44  WDGVKPIPFVISITTGLIIRFAIPKPDNVSPQAWTLLSIFMSTISGLVFAPLPVGAWSFV 103
           W G    P + SI TGLI+ F +P P+ V+  AW LL+IF++TI G++  PLP+GA + +
Sbjct: 87  WQGAAIKPLLASIATGLILWF-VPVPEGVTRNAWQLLAIFLATIVGIITQPLPLGAVALM 146

Query: 104 CLTATLITNTLSFKEAFSAMTNEVIWLIVVAFFFSRGFVKTGLGDRVATCFIKWMGRRTV 163
            L A+++T TL+F  AFSA  + + WLI +AFFF+RGF+KTGLG+RVA  F++  G  ++
Sbjct: 147 GLGASVLTKTLTFAAAFSAFGDPIPWLIALAFFFARGFIKTGLGNRVAYQFVRLFGSSSL 206

Query: 164 GLAYGLVLSEALICPAIPSSTARAGGVFSPVIKSVAVNNGSMPFDNKSGKKIGSYLVMAQ 223
           GL Y LV SEAL+ PAIPS +ARAGG+F P++KS+ V  GS   D  +  ++GS+L++  
Sbjct: 207 GLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCVACGSNVGDG-TEHRLGSWLMLTC 266

Query: 224 LQVCVYVVVTFL 236
            Q  V     FL
Sbjct: 267 FQTSVISSSMFL 276

The following BLAST results are available for this feature:

BLAST of Spo16424.1 vs. NCBI nr
BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
BLAST of Spo16424.1 vs. TAIR (Arabidopsis)

BLAST of Spo16424.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902233151\|gb\|KNA22944.1\|	8.4e-132	99.5	hypothetical protein SOVF_0293...	[more]
gi\|870849289\|gb\|KMT01552.1\|	1.9e-83	69.6	hypothetical protein BVRB_9g21...	[more]
gi\|698569022\|ref\|XP_009774220.1\|	3.1e-65	62.6	PREDICTED: dicarboxylate trans...	[more]
gi\|697135458\|ref\|XP_009621784.1\|	8.9e-65	62.6	PREDICTED: dicarboxylate trans...	[more]
gi\|658022225\|ref\|XP_008346519.1\|	7.5e-64	65.3	PREDICTED: dicarboxylate trans...	[more]

BLAST of Spo16424.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RVK3_SPIOL	5.9e-132	99.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8BKM9_BETVU	1.3e-83	69.6	Uncharacterized protein OS=Bet...	[more]
A0A0C9S8S9_9SPER	5.6e-66	66.3	TSA: Wollemia nobilis Ref_Woll...	[more]
M5VJG8_PRUPE	2.0e-63	66.1	Uncharacterized protein OS=Pru...	[more]
M1AXS7_SOLTU	5.8e-63	64.8	Uncharacterized protein OS=Sol...	[more]

BLAST of Spo16424.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
DIT21_ARATH	3.4e-64	64.4	Dicarboxylate transporter 2.1,...	[more]
DIT2_SPIOL	8.3e-63	60.5	Dicarboxylate transporter 2, c...	[more]
DIT22_ARATH	4.0e-57	59.4	Dicarboxylate transporter 2.2,...	[more]
DIT1_SPIOL	6.8e-41	43.6	Dicarboxylate transporter 1, c...	[more]
DIT1_ARATH	3.4e-40	47.4	Dicarboxylate transporter 1, c...	[more]

BLAST of Spo16424.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G64290.1	1.9e-65	64.4	dicarboxylate transport 2.1	[more]
AT5G64280.1	2.2e-58	59.4	dicarboxylate transporter 2.2	[more]
AT5G12860.1	1.9e-41	47.4	dicarboxylate transporter 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001898	Sodium/sulphate symporter	PFAM	PF00939	Na_sulph_symp	coord: 55..233 score: 7.2
None	No IPR available	PANTHER	PTHR10283	SOLUTE CARRIER FAMILY 13 MEMBER	coord: 43..225 score: 2.9
None	No IPR available	PANTHER	PTHR10283:SF106	SUBFAMILY NOT NAMED	coord: 43..225 score: 2.9

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006814	sodium ion transport
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0019676	ammonia assimilation cycle
biological_process	GO:0015813	L-glutamate transmembrane transport
biological_process	GO:0071423	malate transmembrane transport
biological_process	GO:1902356	oxaloacetate(2-) transmembrane transport
biological_process	GO:0009624	response to nematode
cellular_component	GO:0016020	membrane
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0009534	chloroplast thylakoid
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0005313	L-glutamate transmembrane transporter activity
molecular_function	GO:0015131	oxaloacetate transmembrane transporter activity
molecular_function	GO:0015367	oxoglutarate:malate antiporter activity