Spo21191.1 (mRNA) | SpinachBase

Overview


Name	Spo21191.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Cytochrome b5
Location	SpoScf_01238 : 150159 .. 153423 (-)
Sequence length	1342

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACAAATGTTGTGAATTTGCAAGGGCGTGTGGTTGGTTGATAAGCACATCAATTAAAAAGGGCAACAAAATTCAGGTTCACCTAACCCCTCTAAATAAACCCCATTCAAGATTCAACTTCCTTTGTTCTTTCTTGTTCGGCCCACCAACCAATTTTCAATTCTTTTTCCCCAACTTTCAAATCTCACTAACCCACCCACTATTTTGCTCTTCTCCTTCGAAATCAAAGCTTACCCAGATTGAGCTGAATATTAGTTTTATTCGGATCTGTTTTTCTGGGCAAAAGGGCTGTTTTCTTTTTCACGATTTGTTGAACAAAATCAAATCGAATGGGTAATGAAGGAAAAGTGTTAACTTTGGCCGATGTTTCTGAACACAACTCTTCTAAAGATTGCTGGCTTGTTATCAGTGGAAAGGTTTAATCTCTTTCTCTTCTGTTGATTTAATATCTGAACTTTTAGCTGGTTTTATTGAACTGATCACTTGTTACAATTAGAATTTTCATGGTTTGATTATTTAGTTGATTATTAATTGTGGGTCAGATTTATTTTTCAGCAATCGTATTGAGCTAAGTTATGTGTATTTTCTCTTTTTTATTCAAATATGATGATGTAAAGTTGATCACTTGTTGAAATTTGCATGGTTTGGCTATTTGTTGGATTTTCAAGTTAGGTTTGACTGTTCTGGATAATATGTTAAATGTATGCTAGTGGGTTGAATTTGGAAATTTGATTTGATTTATACTCTTGATATTTATACTCTTGATAAAGGGATCCTGATTCAAAGTTGTTAGAACAATGAGAAATTATGGTATGCTTGTGATTTGCATGCATGTGGTTGGCATTGTGGAGCTGTGGTGGGTATGATTCGATTTCGAATGTGGGGTTATTAGTATTACCTAAATTTAAAAGAACAATGATCAAAGATTTTACTTTCTTTTAGGGCATGCTTGGATTGAGAGTAATGTATTAAAGGGGTAATAAAAGTCAACTAAGGTAATTGAAGGTGATGATGAGGGGATGAAGTGGTGGCAAAGGGAACACGCTAGTGTTGGCAAGAAGAAAATAAAAGGGAAGGGGGAACAAATACCCTCATCATGGGGGGAATAGTTACCCACCATCCCACTAGGGTGATTATTACCCTCATTTGGGGGGTAATTACTTCCCCCCTTCCTCCCTTCTCCTCACAACACCACCACTACCACAACTTCTCATCACAATACCACCACACCACCCTCCTTGCAACCACCTCAATTCACCTTCATTGTCCTTTTATTATGGTGGTTTGACTTTTATTACCCCCATAATCCATTACACCCAATCCAAGCGTGCCCTTATAAACAAAGAAGTATCAGTGGTGAAGTTGGGGGATATGCATCTTTGTTCCTGGATTTTGTCTGATATGAAAGGGCGATGTGTTACCTTATACATCCGGGTGTACCATAGGTTGACATATAGAATACGAATTGTATCAGGGCTATTGTTGTCACTAGCCTTTTTTGAGGCCAAATGATAGTTCAAATGGATAAACTCATCAACTATTTCGTCTAAAGTCACTGAAAAACCTCTTGGTAAATTGTCTGTCTAATTGGTTTTAACTTTCACTAACCTATCACATTCCTCTTGTTGGTTTGATAAATGCTGGTGTAGAAATTGTTACAAAATGTTGAATGTGGAAGTTCTTTAAAAGGAGTAATGAATGGTTTTAACGAGAGATCAAAGCCAGCATCTCGGGGAAATAGTTGAGAGTCTTGAGATCATCCTTGGGTTGTTTGAACTGTTAACGGAGGCTTGGTTTAGTTTAATCATTTCAGATTTTCTTCCCTTTTTGTTGACTTTTGGAGGTCTATTTTATTGGCCGTTGGAAGAAGGCATAAGGTGTGCCGGGCATGCATTACTGAATTACATTTGACTTAGGAGTCGCTTGACAATGGGGTATCTATGGGGTATCTATGTCTAACATGTAACAGATGATTTCTTTACCCTTTGGCATCGACTGTTTTTTATTTGATGTATCTAAGGTTTTAACATATGCTATAACAGGTATATGACGTGACCAAGTTTTTGGAAGACCATCCTGGTGGCGATGAGGTGCTGTTGTCTGCCACAGGTAGTAAAGTGTCACTTTATGCACAATTATTATGAACCCAACTTTTTTTAAATCAAACTTTGATTTGAGGGGGTTGATTTCCTTGTAAGATGTCTGAATGTTGGAACTGAAAGCAAATCAGAGTGTAAGATGCTCTTATTCAACTTGTATAAGGACATTAGGAGTATTACGTTAATCAGAGTGAAAATTTGATCGGCATCAAAGCCACAAGTTAATACTTTTATAACCGTTGCATCATTATGTTGTAGCTTCTGACTGCTAATACAGATCATAACTATGGATTAATGGTATATGCAGGAAAAGATGCAACGGATGATTTTGAGGATGTGGGTCACAGCAGCACTGCTAGAGCTATGATGGACGAGATGTACGTTGGTGACATTGATACTGCTACTATCCCATCCAAGGTTAAATACACACCACCAAAACAACCACAGTACAACCACGACAAGACTCCAGAGTTCGTAATCAAGATCCTTCAGTTCCTAGTCCCCCTCTTAATTTTGGGTTTCGCTGTTGCCATGCGCTATTACACCAAGCAATCATCCGAATAAACTATAAGGCGTACTCCTTCTGAGAAAACAAAGATGATCTTCTATAAGAAAGTAGTATATATGGATAATGGAGCCACTTCCTACCAATTAACAAATGTCTCTTCACAGAGATTAGCAGGATATGTACTTTAATTTCTTCAGCTTCTTATTTTGTGTTTCTCATCTCCTTTCCTTCCCCCCTTTCCCCCAATTTTTCTGTGTGATTTGAAAGTATAAGTAATTTGTATTCCGGAATCTATTACCACACCGTCTGAGCTATAAACTTCAGGTCCTTGGAAACATCAATTTTCTGTCATTGCTTTTTATTAGCAGCAAATTTGTTTAGACCAATCTGGTCTTCTTTCTTTGTTTACTTCCCCTCAATCTTAAGTCTTAACCATTAGTTATGAATTTATCATGCATAGTGAAACGTTGTGCAAAACTAGCTGAAAACGGAAATTTTCAGCTGAAACATATGGTATCATGTAAAAAGAACTTTTCTTTCTTTTATTGAATGAGGGCCTCATTTTGAGAAATTGCACAGGTGCCCCATAAATATCATGGCGTCATCGTAACCCCGACAATGCAGTGGTGAATGAAAGGGGATGGCGTCATCGTAACCCC

mRNA sequence

CACAAATGTTGTGAATTTGCAAGGGCGTGTGGTTGGTTGATAAGCACATCAATTAAAAAGGGCAACAAAATTCAGGTTCACCTAACCCCTCTAAATAAACCCCATTCAAGATTCAACTTCCTTTGTTCTTTCTTGTTCGGCCCACCAACCAATTTTCAATTCTTTTTCCCCAACTTTCAAATCTCACTAACCCACCCACTATTTTGCTCTTCTCCTTCGAAATCAAAGCTTACCCAGATTGAGCTGAATATTAGTTTTATTCGGATCTGTTTTTCTGGGCAAAAGGGCTGTTTTCTTTTTCACGATTTGTTGAACAAAATCAAATCGAATGGGTAATGAAGGAAAAGTGTTAACTTTGGCCGATGTTTCTGAACACAACTCTTCTAAAGATTGCTGGCTTGTTATCAGTGGAAAGGTATATGACGTGACCAAGTTTTTGGAAGACCATCCTGGTGGCGATGAGGTGCTGTTGTCTGCCACAGGAAAAGATGCAACGGATGATTTTGAGGATGTGGGTCACAGCAGCACTGCTAGAGCTATGATGGACGAGATGTACGTTGGTGACATTGATACTGCTACTATCCCATCCAAGGTTAAATACACACCACCAAAACAACCACAGTACAACCACGACAAGACTCCAGAGTTCGTAATCAAGATCCTTCAGTTCCTAGTCCCCCTCTTAATTTTGGGTTTCGCTGTTGCCATGCGCTATTACACCAAGCAATCATCCGAATAAACTATAAGGCGTACTCCTTCTGAGAAAACAAAGATGATCTTCTATAAGAAAGTAGTATATATGGATAATGGAGCCACTTCCTACCAATTAACAAATGTCTCTTCACAGAGATTAGCAGGATATGTACTTTAATTTCTTCAGCTTCTTATTTTGTGTTTCTCATCTCCTTTCCTTCCCCCCTTTCCCCCAATTTTTCTGTGTGATTTGAAAGTATAAGTAATTTGTATTCCGGAATCTATTACCACACCGTCTGAGCTATAAACTTCAGGTCCTTGGAAACATCAATTTTCTGTCATTGCTTTTTATTAGCAGCAAATTTGTTTAGACCAATCTGGTCTTCTTTCTTTGTTTACTTCCCCTCAATCTTAAGTCTTAACCATTAGTTATGAATTTATCATGCATAGTGAAACGTTGTGCAAAACTAGCTGAAAACGGAAATTTTCAGCTGAAACATATGGTATCATGTAAAAAGAACTTTTCTTTCTTTTATTGAATGAGGGCCTCATTTTGAGAAATTGCACAGGTGCCCCATAAATATCATGGCGTCATCGTAACCCCGACAATGCAGTGGTGAATGAAAGGGGATGGCGTCATCGTAACCCC

Coding sequence (CDS)

ATGGGTAATGAAGGAAAAGTGTTAACTTTGGCCGATGTTTCTGAACACAACTCTTCTAAAGATTGCTGGCTTGTTATCAGTGGAAAGGTATATGACGTGACCAAGTTTTTGGAAGACCATCCTGGTGGCGATGAGGTGCTGTTGTCTGCCACAGGAAAAGATGCAACGGATGATTTTGAGGATGTGGGTCACAGCAGCACTGCTAGAGCTATGATGGACGAGATGTACGTTGGTGACATTGATACTGCTACTATCCCATCCAAGGTTAAATACACACCACCAAAACAACCACAGTACAACCACGACAAGACTCCAGAGTTCGTAATCAAGATCCTTCAGTTCCTAGTCCCCCTCTTAATTTTGGGTTTCGCTGTTGCCATGCGCTATTACACCAAGCAATCATCCGAATAA

Protein sequence

MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFEDVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLILGFAVAMRYYTKQSSE

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo21191	Spo21191	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo21191.1	Spo21191.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21191.1.utr5p.1	Spo21191.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21191.1.CDS.3	Spo21191.1.CDS.3	CDS
Spo21191.1.CDS.2	Spo21191.1.CDS.2	CDS
Spo21191.1.CDS.1	Spo21191.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21191.1.utr3p.1	Spo21191.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21191.1.exon.3	Spo21191.1.exon.3	exon
Spo21191.1.exon.2	Spo21191.1.exon.2	exon
Spo21191.1.exon.1	Spo21191.1.exon.1	exon

Homology

[Close]

BLAST of Spo21191.1 vs. NCBI nr
Match: gi|902146676|gb|KNA05090.1| (hypothetical protein SOVF_193610 [Spinacia oleracea])

HSP 1 Score: 278.9 bits (712), Expect = 4.800e-72
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI
Sbjct: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120

Query: 121 LGFAVAMRYYTKQSSE 137
           LGFAVAMRYYTKQSSE
Sbjct: 121 LGFAVAMRYYTKQSSE 136

[Close]

BLAST of Spo21191.1 vs. NCBI nr
Match: gi|731347475|ref|XP_010685005.1| (PREDICTED: cytochrome b5 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 256.9 bits (655), Expect = 2.000e-65
Identity = 121/136 (88.97%), Postives = 132/136 (97.06%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG EGKV +L++V+EHNSSKDCWLVI GKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGEGKVFSLSEVAEHNSSKDCWLVIGGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSSTARAMMDEMYVGDI+TATIP+KV+YTPPKQPQYNHDKT EF+IKILQFLVP+LI
Sbjct: 61  DVGHSSTARAMMDEMYVGDINTATIPTKVRYTPPKQPQYNHDKTSEFIIKILQFLVPILI 120

Query: 121 LGFAVAMRYYTKQSSE 137
           LGFAVAMR+YTKQS+E
Sbjct: 121 LGFAVAMRFYTKQSTE 136

[Close]

BLAST of Spo21191.1 vs. NCBI nr
Match: gi|255560157|ref|XP_002521096.1| (PREDICTED: cytochrome b5 [Ricinus communis])

HSP 1 Score: 236.9 bits (603), Expect = 2.100e-59
Identity = 111/134 (82.84%), Postives = 122/134 (91.04%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG +GKV TLADVSEHNS KDCWLVI GKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGQGKVYTLADVSEHNSPKDCWLVIEGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAMMDE YVG+IDT+TIP+K  YTPPKQP YN DKTPEF+IK+LQF+VPLLI
Sbjct: 61  DVGHSSSARAMMDEFYVGEIDTSTIPTKKAYTPPKQPHYNQDKTPEFIIKLLQFVVPLLI 120

Query: 121 LGFAVAMRYYTKQS 135
           LG AV +R+YTK +
Sbjct: 121 LGLAVGIRFYTKSA 134

[Close]

BLAST of Spo21191.1 vs. NCBI nr
Match: gi|702434464|ref|XP_010069657.1| (PREDICTED: cytochrome b5 [Eucalyptus grandis])

HSP 1 Score: 234.2 bits (596), Expect = 1.400e-58
Identity = 109/134 (81.34%), Postives = 122/134 (91.04%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG EGKV TLA VSEHN+SKDCWLVI GKVYDVTKF+EDHPGGDEVLLS+TGKDATDDFE
Sbjct: 1   MGGEGKVYTLAQVSEHNTSKDCWLVIDGKVYDVTKFMEDHPGGDEVLLSSTGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAMMD+ YVG+ID +TIP++ KYTPPKQPQYN DKTPEFVIK+LQFLVPLLI
Sbjct: 61  DVGHSSSARAMMDDFYVGEIDASTIPARTKYTPPKQPQYNPDKTPEFVIKLLQFLVPLLI 120

Query: 121 LGFAVAMRYYTKQS 135
           LG A  +R+YTK +
Sbjct: 121 LGLAFGVRFYTKSA 134

[Close]

BLAST of Spo21191.1 vs. NCBI nr
Match: gi|928193496|gb|ALE65998.1| (cytochrome b5 [Camellia sinensis var. sinensis])

HSP 1 Score: 234.2 bits (596), Expect = 1.400e-58
Identity = 110/134 (82.09%), Postives = 122/134 (91.04%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG +GKV TLA+VSEHN+ KDCWLVI GKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGDGKVFTLAEVSEHNTPKDCWLVIDGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAMMDE YVGDID+A+IPSK KYTPPKQP YN DKT EF+IK+LQFLVPL+I
Sbjct: 61  DVGHSSSARAMMDEFYVGDIDSASIPSKKKYTPPKQPHYNQDKTTEFIIKLLQFLVPLVI 120

Query: 121 LGFAVAMRYYTKQS 135
           LG AV +R+YTK +
Sbjct: 121 LGVAVGIRFYTKSA 134

[Close]

BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QCV8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_193610 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 3.400e-72
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI
Sbjct: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120

Query: 121 LGFAVAMRYYTKQSSE 137
           LGFAVAMRYYTKQSSE
Sbjct: 121 LGFAVAMRYYTKQSSE 136

[Close]

BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BVI8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g167560 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 1.400e-65
Identity = 121/136 (88.97%), Postives = 132/136 (97.06%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG EGKV +L++V+EHNSSKDCWLVI GKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGEGKVFSLSEVAEHNSSKDCWLVIGGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSSTARAMMDEMYVGDI+TATIP+KV+YTPPKQPQYNHDKT EF+IKILQFLVP+LI
Sbjct: 61  DVGHSSTARAMMDEMYVGDINTATIPTKVRYTPPKQPQYNHDKTSEFIIKILQFLVPILI 120

Query: 121 LGFAVAMRYYTKQSSE 137
           LGFAVAMR+YTKQS+E
Sbjct: 121 LGFAVAMRFYTKQSTE 136

[Close]

BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
Match: B9S527_RICCO (Cytochrome B5 isoform 1, putative OS=Ricinus communis GN=RCOM_1720370 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.500e-59
Identity = 111/134 (82.84%), Postives = 122/134 (91.04%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG +GKV TLADVSEHNS KDCWLVI GKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGQGKVYTLADVSEHNSPKDCWLVIEGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAMMDE YVG+IDT+TIP+K  YTPPKQP YN DKTPEF+IK+LQF+VPLLI
Sbjct: 61  DVGHSSSARAMMDEFYVGEIDTSTIPTKKAYTPPKQPHYNQDKTPEFIIKLLQFVVPLLI 120

Query: 121 LGFAVAMRYYTKQS 135
           LG AV +R+YTK +
Sbjct: 121 LGLAVGIRFYTKSA 134

[Close]

BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
Match: A0A059AW33_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00800 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 9.500e-59
Identity = 109/134 (81.34%), Postives = 122/134 (91.04%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG EGKV TLA VSEHN+SKDCWLVI GKVYDVTKF+EDHPGGDEVLLS+TGKDATDDFE
Sbjct: 1   MGGEGKVYTLAQVSEHNTSKDCWLVIDGKVYDVTKFMEDHPGGDEVLLSSTGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAMMD+ YVG+ID +TIP++ KYTPPKQPQYN DKTPEFVIK+LQFLVPLLI
Sbjct: 61  DVGHSSSARAMMDDFYVGEIDASTIPARTKYTPPKQPQYNPDKTPEFVIKLLQFLVPLLI 120

Query: 121 LGFAVAMRYYTKQS 135
           LG A  +R+YTK +
Sbjct: 121 LGLAFGVRFYTKSA 134

[Close]

BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
Match: A0A0M4P3X0_CAMSI (Cytochrome b5 OS=Camellia sinensis var. sinensis PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 9.500e-59
Identity = 110/134 (82.09%), Postives = 122/134 (91.04%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG +GKV TLA+VSEHN+ KDCWLVI GKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGDGKVFTLAEVSEHNTPKDCWLVIDGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAMMDE YVGDID+A+IPSK KYTPPKQP YN DKT EF+IK+LQFLVPL+I
Sbjct: 61  DVGHSSSARAMMDEFYVGDIDSASIPSKKKYTPPKQPHYNQDKTTEFIIKLLQFLVPLVI 120

Query: 121 LGFAVAMRYYTKQS 135
           LG AV +R+YTK +
Sbjct: 121 LGVAVGIRFYTKSA 134

[Close]

BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.900e-60
Identity = 109/135 (80.74%), Postives = 123/135 (91.11%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG E KV TLA+VS+HN++KDCWLVISGKVYDVTKFL+DHPGGDEVLLSATGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHSS+ARAM+DE YVGDID+ATIP+K KYTPP QP YN DKT EFV+K+LQFLVPL+I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGFAVAMRYYTKQSS 136
           LG A  +R+YTKQSS
Sbjct: 121 LGVAFGIRFYTKQSS 135

[Close]

BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.800e-58
Identity = 103/135 (76.30%), Postives = 122/135 (90.37%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG + KV TLA+VS HN++KDCWL+ISGKVY+VTKFLEDHPGG EVLLSATGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           D+GHSS+ARAM+DE YVGDID++TIP+KVKYTPPKQP YN DKT EF++K+LQFLVPL+I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGFAVAMRYYTKQSS 136
           LG A  + +YTKQS+
Sbjct: 121 LGVAFGVHFYTKQSA 135

[Close]

BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
Match: CYB5_BOROF (Cytochrome b5 OS=Borago officinalis PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.300e-56
Identity = 100/131 (76.34%), Postives = 122/131 (93.13%), Query Frame = 1

		  
Query: 5   GKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFEDVGH 64
           GK+ TLA+V++HN+SKDCWL+I+GKVYDVTKFLEDHPGGD+VLLSATGKDATDDFED+GH
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

Query: 65  SSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLILGFA 124
           SS+A+AM+DE YVGDID+++IPS+VKYTPPKQP YN DKT EFVIK+LQFLVPL+IL  A
Sbjct: 62  SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGA 121

Query: 125 VAMRYYTKQSS 136
           + +R+YTK S+
Sbjct: 122 IGIRFYTKSSA 132

[Close]

BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 218.4 bits (555), Expect = 4.800e-56
Identity = 103/136 (75.74%), Postives = 118/136 (86.76%), Query Frame = 1

		  
Query: 1   MGNEGK-VLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDF 60
           M N+ K V TL +V++HNS  DCWL+I GKVY+V+KFLEDHPGGD+VLLS+TGKDATDDF
Sbjct: 1   MSNDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDF 60

Query: 61  EDVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLL 120
           EDVGHS+TARAMMDE YVGDIDT+TIP++ KY PPKQP YN DKTPEF+IKILQFLVPL 
Sbjct: 61  EDVGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLA 120

Query: 121 ILGFAVAMRYYTKQSS 136
           ILG AVA+R YTK  S
Sbjct: 121 ILGLAVAIRIYTKSES 136

[Close]

BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.400e-55
Identity = 98/132 (74.24%), Postives = 115/132 (87.12%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG+E K+ TL++VSEHN + DCW+VI+GKVY+VTKFLEDHPGGD+VLLS+TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHS +AR MM++ YVG+ID  TIP KVKYTPPKQP YN DKT EF+IK+LQFLVPL I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGFAVAMRYYTK 133
           LG AV +R YTK
Sbjct: 121 LGLAVGIRIYTK 132

[Close]

BLAST of Spo21191.1 vs. TAIR (Arabidopsis)
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 214.9 bits (546), Expect = 3.000e-56
Identity = 98/132 (74.24%), Postives = 115/132 (87.12%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG+E K+ TL++VSEHN + DCW+VI+GKVY+VTKFLEDHPGGD+VLLS+TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHS +AR MM++ YVG+ID  TIP KVKYTPPKQP YN DKT EF+IK+LQFLVPL I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGFAVAMRYYTK 133
           LG AV +R YTK
Sbjct: 121 LGLAVGIRIYTK 132

[Close]

BLAST of Spo21191.1 vs. TAIR (Arabidopsis)
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 204.1 bits (518), Expect = 5.300e-53
Identity = 95/134 (70.90%), Postives = 115/134 (85.82%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           MG +GKV TL++VS+H+S+KDCW+VI GKVYDVTKFL+DHPGGDEV+L++TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQ--YNHDKTPEFVIKILQFLVPL 120
           DVGHSSTA+AM+DE YVGDIDTAT+P K K+ PP   +     DK+ +FVIK+LQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LILGFAVAMRYYTK 133
           LILG A  +RYYTK
Sbjct: 121 LILGLAFGIRYYTK 134

[Close]

BLAST of Spo21191.1 vs. TAIR (Arabidopsis)
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 199.1 bits (505), Expect = 1.700e-51
Identity = 88/133 (66.17%), Postives = 115/133 (86.47%), Query Frame = 1

		  
Query: 1   MGNEGKVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60
           M ++ KVL+  +VS+HN +KDCWL+ISGKVYDVT F++DHPGGDEVLLS+TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSSTARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLI 120
           DVGHS TAR MMD+ ++G+ID++++P+   Y  P+QP YN DKTPEF+IKILQFLVP+LI
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGFAVAMRYYTKQ 134
           LG A+ +R+YTK+
Sbjct: 121 LGLALVVRHYTKK 133

[Close]

BLAST of Spo21191.1 vs. TAIR (Arabidopsis)
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 128.6 bits (322), Expect = 2.800e-30
Identity = 58/129 (44.96%), Postives = 89/129 (68.99%), Query Frame = 1

		  
Query: 7   VLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFEDVGHSS 66
           +++  DV++H    DCW++I GKVYD++ F+++HPGGD VLL+ TGKDA+ DFEDV HS 
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  TARAMMDEMYVGDIDTATIPSKVKYTPPKQPQYNHDKT--PEFVIKILQFLVPLLILGFA 126
            A+ +M +  +GD+D +T+P   +Y PP + +    +T   E   K+L +L+PLLILG A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VAMRYYTKQ 134
            A+R+Y  +
Sbjct: 124 FALRFYNNK 132

[Close]

BLAST of Spo21191.1 vs. TAIR (Arabidopsis)
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 113.2 bits (282), Expect = 1.200e-25
Identity = 52/124 (41.94%), Postives = 85/124 (68.55%), Query Frame = 1

		  
Query: 6   KVLTLADVSEHNSSKDCWLVISGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFEDVGHS 65
           K+ ++ + + HN   DCW+VI GKVYDV+ ++++HPGGD+VLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  STARAMMDEMYVGDIDTATIPS--KVKYTPPKQPQYNHDKTPEFVIKILQFLVPLLILGF 125
             AR +M++ ++G++D +++P   ++K     QPQ +  K   F +    ++VP+ I+  
Sbjct: 66  KDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKL--FDLTKQYWVVPVSIITI 125

Query: 126 AVAM 128
           +VA+
Sbjct: 126 SVAV 127

The following BLAST results are available for this feature:

BLAST of Spo21191.1 vs. NCBI nr
BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
BLAST of Spo21191.1 vs. TAIR (Arabidopsis)

BLAST of Spo21191.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|902146676\|gb\|KNA05090.1\|	4.8e-72	100.	hypothetical protein SOVF_1936...	[more]
gi\|731347475\|ref\|XP_010685005.1\|	2.0e-65	88.9	PREDICTED: cytochrome b5 [Beta...	[more]
gi\|255560157\|ref\|XP_002521096.1\|	2.1e-59	82.8	PREDICTED: cytochrome b5 [Rici...	[more]
gi\|702434464\|ref\|XP_010069657.1\|	1.4e-58	81.3	PREDICTED: cytochrome b5 [Euca...	[more]
gi\|928193496\|gb\|ALE65998.1\|	1.4e-58	82.0	cytochrome b5 [Camellia sinens...	[more]

BLAST of Spo21191.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
A0A0K9QCV8_SPIOL	3.4e-72	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8BVI8_BETVU	1.4e-65	88.9	Uncharacterized protein OS=Bet...	[more]
B9S527_RICCO	1.5e-59	82.8	Cytochrome B5 isoform 1, putat...	[more]
A0A059AW33_EUCGR	9.5e-59	81.3	Uncharacterized protein OS=Euc...	[more]
A0A0M4P3X0_CAMSI	9.5e-59	82.0	Cytochrome b5 OS=Camellia sine...	[more]

BLAST of Spo21191.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
CYB5_TOBAC	1.9e-60	80.7	Cytochrome b5 OS=Nicotiana tab...	[more]
CYB5S_TOBAC	1.8e-58	76.3	Cytochrome b5, seed isoform OS...	[more]
CYB5_BOROF	1.3e-56	76.3	Cytochrome b5 OS=Borago offici...	[more]
CYB5_ORYSJ	4.8e-56	75.7	Cytochrome b5 OS=Oryza sativa ...	[more]
CYB5B_ARATH	5.4e-55	74.2	Cytochrome b5 isoform B OS=Ara...	[more]

BLAST of Spo21191.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
AT2G32720.1	3.0e-56	74.2	cytochrome B5 isoform B	[more]
AT5G48810.1	5.3e-53	70.9	cytochrome B5 isoform D	[more]
AT5G53560.1	1.7e-51	66.1	cytochrome B5 isoform E	[more]
AT2G46650.1	2.8e-30	44.9	cytochrome B5 isoform C	[more]
AT1G26340.1	1.2e-25	41.9	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 1.2E-15coord: 55..62 score: 1.2E-15coord: 68..80 score: 1.2E-15coord: 30..40 score: 1.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	3.10.120.10		coord: 5..88 score: 6.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..80 score: 8.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 score: 5.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..89 score: 1.06
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..136 score: 7.2
None	No IPR available	PANTHER	PTHR19359:SF26	CYTOCHROME B5 ISOFORM B	coord: 1..136 score: 7.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019745	pentacyclic triterpenoid biosynthetic process
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009325	nitrate reductase complex
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0009703	nitrate reductase (NADH) activity