Spo10685.1 (mRNA) | SpinachBase

Overview


Name	Spo10685.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	RING/U-box superfamily protein, putative
Location	chr1 : 41597752 .. 41598462 (+)
Sequence length	711

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCAGAACCAGACGTTTCCGCAGAAGAGATGCAATCGTTTTTAGAAAGGCTAATAAGTTCAAGAAACAGAGACCTCTCTTCGTTCCTCCCATTTCTTTTAGGGTTCCCAATTTCTGACCCAACTCAGACTCGGTCTAACTCGGGTGAAAACTCGCCGAATCATCCGACCCCAGAAGGCCAACCACGGGAGAGGATCGTTCTGATCAACCCTTTGACGCAAGGGATGGTGGTGATTGAAGGGACAGGGCTTGATTCGCTGTTTCACGGCGTACAGAGTATTAAAGATGGGCAGCCACCGGCGTCGAAATCGGCGATTGAAGCCATGCCATGTGTCGAGATCAGAGAGGACGATGAGGAGAGAGAGTGTGTGATTTGTTTGGAAGTGTGTGGACCCAGTGTCGGGGTTGTGAAGGAGATGCCATGTAAGCATAGATTTCACGGCGAGTGTATTGAGAAGTGGTTGGGGATTCATGGTTCGTGCCCGGTTTGCCGGTTCAAGATGCCAGTCGAGGAGGAAGATGGCGGGAAGAAAGGAGAAGAGGAGGAGAGTGGAGAGAGAAGGACGGAGATTTGGGTTAGTGTTTCGTTTCGGCGTGGTAGGAGAAGAACAGAGGAAGAGGCGGTTGAGGAGAGAGAAAGTGGCAGTAATGATGAGTTGAATACAGGAGATTCCGATTCTGGTAACGAGAATGAAGGAGAATCGTAG

mRNA sequence

ATGGCGTCAGAACCAGACGTTTCCGCAGAAGAGATGCAATCGTTTTTAGAAAGGCTAATAAGTTCAAGAAACAGAGACCTCTCTTCGTTCCTCCCATTTCTTTTAGGGTTCCCAATTTCTGACCCAACTCAGACTCGGTCTAACTCGGGTGAAAACTCGCCGAATCATCCGACCCCAGAAGGCCAACCACGGGAGAGGATCGTTCTGATCAACCCTTTGACGCAAGGGATGGTGGTGATTGAAGGGACAGGGCTTGATTCGCTGTTTCACGGCGTACAGAGTATTAAAGATGGGCAGCCACCGGCGTCGAAATCGGCGATTGAAGCCATGCCATGTGTCGAGATCAGAGAGGACGATGAGGAGAGAGAGTGTGTGATTTGTTTGGAAGTGTGTGGACCCAGTGTCGGGGTTGTGAAGGAGATGCCATGTAAGCATAGATTTCACGGCGAGTGTATTGAGAAGTGGTTGGGGATTCATGGTTCGTGCCCGGTTTGCCGGTTCAAGATGCCAGTCGAGGAGGAAGATGGCGGGAAGAAAGGAGAAGAGGAGGAGAGTGGAGAGAGAAGGACGGAGATTTGGGTTAGTGTTTCGTTTCGGCGTGGTAGGAGAAGAACAGAGGAAGAGGCGGTTGAGGAGAGAGAAAGTGGCAGTAATGATGAGTTGAATACAGGAGATTCCGATTCTGGTAACGAGAATGAAGGAGAATCGTAG

Coding sequence (CDS)

ATGGCGTCAGAACCAGACGTTTCCGCAGAAGAGATGCAATCGTTTTTAGAAAGGCTAATAAGTTCAAGAAACAGAGACCTCTCTTCGTTCCTCCCATTTCTTTTAGGGTTCCCAATTTCTGACCCAACTCAGACTCGGTCTAACTCGGGTGAAAACTCGCCGAATCATCCGACCCCAGAAGGCCAACCACGGGAGAGGATCGTTCTGATCAACCCTTTGACGCAAGGGATGGTGGTGATTGAAGGGACAGGGCTTGATTCGCTGTTTCACGGCGTACAGAGTATTAAAGATGGGCAGCCACCGGCGTCGAAATCGGCGATTGAAGCCATGCCATGTGTCGAGATCAGAGAGGACGATGAGGAGAGAGAGTGTGTGATTTGTTTGGAAGTGTGTGGACCCAGTGTCGGGGTTGTGAAGGAGATGCCATGTAAGCATAGATTTCACGGCGAGTGTATTGAGAAGTGGTTGGGGATTCATGGTTCGTGCCCGGTTTGCCGGTTCAAGATGCCAGTCGAGGAGGAAGATGGCGGGAAGAAAGGAGAAGAGGAGGAGAGTGGAGAGAGAAGGACGGAGATTTGGGTTAGTGTTTCGTTTCGGCGTGGTAGGAGAAGAACAGAGGAAGAGGCGGTTGAGGAGAGAGAAAGTGGCAGTAATGATGAGTTGAATACAGGAGATTCCGATTCTGGTAACGAGAATGAAGGAGAATCGTAG

Protein sequence

MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPEGQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDEERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKGEEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo10685	Spo10685	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo10685.1	Spo10685.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10685.1.exon.1	Spo10685.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10685.1.CDS.1	Spo10685.1.CDS.1	CDS

Homology

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BLAST of Spo10685.1 vs. NCBI nr
Match: gi|902232948|gb|KNA22859.1| (hypothetical protein SOVF_029680 [Spinacia oleracea])

HSP 1 Score: 470.7 bits (1210), Expect = 1.500e-129
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE
Sbjct: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60

Query: 61  GQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDE 120
           GQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDE
Sbjct: 61  GQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDE 120

Query: 121 ERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKG 180
           ERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKG
Sbjct: 121 ERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKG 180

Query: 181 EEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES 237
           EEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES
Sbjct: 181 EEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES 236

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BLAST of Spo10685.1 vs. NCBI nr
Match: gi|731343984|ref|XP_010683168.1| (PREDICTED: E3 ubiquitin-protein ligase RNF181 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 245.4 bits (625), Expect = 1.000e-61
Identity = 142/229 (62.01%), Postives = 166/229 (72.49%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQ-----TRSNSG--ENS 60
           MASE   SAEEMQS LERLI+SRNRDLS F+PFLLGF  +DPTQ     T S S   +NS
Sbjct: 1   MASE---SAEEMQSILERLINSRNRDLSMFIPFLLGFTSTDPTQNPTQETESGSSPDDNS 60

Query: 61  PNHPTPEGQPR-ERIVLINPLTQGMVVIEGT-----GLDSLFHGVQSIKDGQPPASKSAI 120
            N   P+ + + ERIVLINPLTQGMVVIEGT     G DSLF G    K+GQPPASKSAI
Sbjct: 61  RNQQNPDEENQGERIVLINPLTQGMVVIEGTTTTVEGFDSLFRG----KEGQPPASKSAI 120

Query: 121 EAMPCVEIREDDEERECVICLEVCGPSVG-VVKEMPCKHRFHGECIEKWLGIHGSCPVCR 180
           E++P VEIRE+++E +CVICLE C   +G +VKEMPC+HRFHG CIEKWLGIHG+CPVCR
Sbjct: 121 ESLPGVEIREEEDEDDCVICLEEC--RIGEIVKEMPCEHRFHGGCIEKWLGIHGTCPVCR 180

Query: 181 FKMPVEEEDGGKKGEEEESGERRTEIWVSVSFRRGRRRTEEEAVEERES 216
           FKMPV+EE   K  E E +   + +IWVS  F RGRRRT  +  E  +S
Sbjct: 181 FKMPVDEEGKKKDEERENNNTGQRQIWVSFVFGRGRRRTNGDNSENLDS 220

[Close]

BLAST of Spo10685.1 vs. NCBI nr
Match: gi|1009154331|ref|XP_015895111.1| (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Ziziphus jujuba])

HSP 1 Score: 213.8 bits (543), Expect = 3.300e-52
Identity = 125/228 (54.82%), Postives = 150/228 (65.79%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           MASE D S  E+ +  ERL+  RNRDLS FLPF+ GF  S P     +  E + N  T  
Sbjct: 1   MASEADAS--ELSTMFERLV--RNRDLSLFLPFIFGFANSLPRANPQDPDEENENRATS- 60

Query: 61  GQPRERIVLINPLTQGMVVIEG-TGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDD 120
              R+R++LINP TQGMVVI+G + LDS+   +   KDGQPPASK++IEAMP VE  E  
Sbjct: 61  ---RDRVILINPFTQGMVVIDGFSSLDSVLRDLGG-KDGQPPASKASIEAMPGVETEE-- 120

Query: 121 EERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKK 180
           E  ECVICLE      GV KEMPCKH+FHG+CIEKWLGIHGSCPVCR  MPV+EE+ GKK
Sbjct: 121 EGLECVICLEEFEVG-GVAKEMPCKHKFHGDCIEKWLGIHGSCPVCRHNMPVDEEEMGKK 180

Query: 181 GEEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSD 228
            EEE    RR E+WVS SF  GRR  +   V   +S  ND  ++ D+D
Sbjct: 181 REEEGRERRRGEVWVSFSFNSGRRSEDSNQVSSADSDVNDSTSSPDAD 216

[Close]

BLAST of Spo10685.1 vs. NCBI nr
Match: gi|819320825|gb|AKG50124.1| (E3 ubiquitin-protein ligase, partial [Betula luminifera])

HSP 1 Score: 208.8 bits (530), Expect = 1.100e-50
Identity = 131/224 (58.48%), Postives = 154/224 (68.75%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGF--PISDPTQTRSNSGENSPNHPT 60
           MASE D+S  E+ S  ER++  R+RDLS F+PF+LGF  P  DP Q+     +   N PT
Sbjct: 1   MASEADLS--EISSMFERVV--RDRDLSLFIPFILGFAGPRRDP-QSEDVPDQEPENTPT 60

Query: 61  PEGQPRERIVLINPLTQGMVVIEGTG-LDSLFHGVQSIKDGQPPASKSAIEAMPCVEIRE 120
               PR+RI+LINP TQGMVVI+GT  LDSL    Q  KDGQPPASK++IEA+  VE+ E
Sbjct: 61  T---PRDRIILINPFTQGMVVIDGTSSLDSLLRE-QGGKDGQPPASKASIEALLNVEVDE 120

Query: 121 DDEERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGG 180
           D   RECVICL+  G   GV KEMPCKHRFH  CIEKWLGIHGSCPVCR+KMPVEEE+ G
Sbjct: 121 DG--RECVICLDELGVG-GVAKEMPCKHRFHANCIEKWLGIHGSCPVCRYKMPVEEEEVG 180

Query: 181 KKGEEEESGERRT--EIWVSVSFRRGRRRTEEEAVEERESGSND 220
           KK  +EE  ERR   EIWVS SF  G  RT  ++ +   S SND
Sbjct: 181 KK-RDEEGRERRVEREIWVSFSFNSG--RTSGDSSQTPTSDSND 209

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BLAST of Spo10685.1 vs. NCBI nr
Match: gi|947122535|gb|KRH70741.1| (hypothetical protein GLYMA_02G108300 [Glycine max])

HSP 1 Score: 206.8 bits (525), Expect = 4.000e-50
Identity = 126/241 (52.28%), Postives = 162/241 (67.22%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           MASE +V  + + S  ER    +NRD+S FLPFL GF  SD T  R++   +    PT E
Sbjct: 73  MASETEVP-DIISSVFERF--QQNRDMSLFLPFLFGF--SDATPGRNSDDPDHETAPT-E 132

Query: 61  GQPRERIVLINPLTQGMVVIEG-TGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDD 120
              R+RI+L+NPLTQGMVVI+G + L++LF    + K G+PPASK +IEA+P VEI E +
Sbjct: 133 APQRQRIILVNPLTQGMVVIDGGSSLEALFREFTNGKGGRPPASKESIEALPSVEIGEGN 192

Query: 121 EERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKK 180
           E+ ECV+CLE  G   GV KEMPCKHRFHG CIEKWLG+HGSCPVCR++MPVEE D GKK
Sbjct: 193 EDSECVVCLEEFGVG-GVAKEMPCKHRFHGNCIEKWLGMHGSCPVCRYEMPVEEIDWGKK 252

Query: 181 GEEEESGERRT----EIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGE 237
             EEE GERR     E+WVS SF R  RR ++   ++ +SG +++ +   S  G+    E
Sbjct: 253 -REEEGGERRRSDGGEVWVSFSFNRSSRRNQDH--DQADSGDSNDDDGSSSSHGDGAAEE 303

[Close]

BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RVB4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_029680 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.000e-129
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE
Sbjct: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60

Query: 61  GQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDE 120
           GQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDE
Sbjct: 61  GQPRERIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDDE 120

Query: 121 ERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKG 180
           ERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKG
Sbjct: 121 ERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKG 180

Query: 181 EEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES 237
           EEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES
Sbjct: 181 EEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGES 236

[Close]

BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
Match: A0A0F7G5V7_9ROSI (E3 ubiquitin-protein ligase (Fragment) OS=Betula luminifera GN=RING1 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 7.400e-51
Identity = 131/224 (58.48%), Postives = 154/224 (68.75%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGF--PISDPTQTRSNSGENSPNHPT 60
           MASE D+S  E+ S  ER++  R+RDLS F+PF+LGF  P  DP Q+     +   N PT
Sbjct: 1   MASEADLS--EISSMFERVV--RDRDLSLFIPFILGFAGPRRDP-QSEDVPDQEPENTPT 60

Query: 61  PEGQPRERIVLINPLTQGMVVIEGTG-LDSLFHGVQSIKDGQPPASKSAIEAMPCVEIRE 120
               PR+RI+LINP TQGMVVI+GT  LDSL    Q  KDGQPPASK++IEA+  VE+ E
Sbjct: 61  T---PRDRIILINPFTQGMVVIDGTSSLDSLLRE-QGGKDGQPPASKASIEALLNVEVDE 120

Query: 121 DDEERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGG 180
           D   RECVICL+  G   GV KEMPCKHRFH  CIEKWLGIHGSCPVCR+KMPVEEE+ G
Sbjct: 121 DG--RECVICLDELGVG-GVAKEMPCKHRFHANCIEKWLGIHGSCPVCRYKMPVEEEEVG 180

Query: 181 KKGEEEESGERRT--EIWVSVSFRRGRRRTEEEAVEERESGSND 220
           KK  +EE  ERR   EIWVS SF  G  RT  ++ +   S SND
Sbjct: 181 KK-RDEEGRERRVEREIWVSFSFNSG--RTSGDSSQTPTSDSND 209

[Close]

BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
Match: A0A0B2PS65_GLYSO (E3 ubiquitin-protein ligase RING1 OS=Glycine soja GN=glysoja_031549 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.800e-50
Identity = 126/241 (52.28%), Postives = 162/241 (67.22%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           MASE +V  + + S  ER    +NRD+S FLPFL GF  SD T  R++   +    PT E
Sbjct: 1   MASETEVP-DIISSVFERF--QQNRDMSLFLPFLFGF--SDATPGRNSDDPDHETAPT-E 60

Query: 61  GQPRERIVLINPLTQGMVVIEG-TGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDD 120
              R+RI+L+NPLTQGMVVI+G   L++LF  + + K G+PPASK +IEA+P VEI E +
Sbjct: 61  APQRQRIILVNPLTQGMVVIDGGLSLEALFRELANGKGGRPPASKESIEALPSVEIGEGN 120

Query: 121 EERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKK 180
           E+ ECV+CLE  G   GV KEMPCKHRFHG CIEKWLG+HGSCPVCR++MPVEE D GKK
Sbjct: 121 EDSECVVCLEEFGVG-GVAKEMPCKHRFHGNCIEKWLGMHGSCPVCRYEMPVEEIDWGKK 180

Query: 181 GEEEESGERRT----EIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGE 237
             EEE GERR     E+WVS SF R  RR ++   ++ +SG +++ +   S  G+    E
Sbjct: 181 -REEEGGERRRSDGGEVWVSFSFNRSSRRNQDH--DQADSGDSNDDDGSSSSHGDGAAEE 231

[Close]

BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
Match: I1JE53_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G108300 PE=4 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 2.800e-50
Identity = 126/241 (52.28%), Postives = 162/241 (67.22%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           MASE +V  + + S  ER    +NRD+S FLPFL GF  SD T  R++   +    PT E
Sbjct: 73  MASETEVP-DIISSVFERF--QQNRDMSLFLPFLFGF--SDATPGRNSDDPDHETAPT-E 132

Query: 61  GQPRERIVLINPLTQGMVVIEG-TGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDD 120
              R+RI+L+NPLTQGMVVI+G + L++LF    + K G+PPASK +IEA+P VEI E +
Sbjct: 133 APQRQRIILVNPLTQGMVVIDGGSSLEALFREFTNGKGGRPPASKESIEALPSVEIGEGN 192

Query: 121 EERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKK 180
           E+ ECV+CLE  G   GV KEMPCKHRFHG CIEKWLG+HGSCPVCR++MPVEE D GKK
Sbjct: 193 EDSECVVCLEEFGVG-GVAKEMPCKHRFHGNCIEKWLGMHGSCPVCRYEMPVEEIDWGKK 252

Query: 181 GEEEESGERRT----EIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEGE 237
             EEE GERR     E+WVS SF R  RR ++   ++ +SG +++ +   S  G+    E
Sbjct: 253 -REEEGGERRRSDGGEVWVSFSFNRSSRRNQDH--DQADSGDSNDDDGSSSSHGDGAAEE 303

[Close]

BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
Match: A0A151RS88_CAJCA (E3 ubiquitin-protein ligase rnf181 family OS=Cajanus cajan GN=KK1_033057 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.400e-49
Identity = 119/229 (51.97%), Postives = 150/229 (65.50%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           M+SE +V   ++ S  +R    RNRD+S FLPFL GF  SDP +    SG+     P  E
Sbjct: 1   MSSEAEVP--DISSLFQRF--QRNRDMSLFLPFLFGF--SDPGRA---SGDPDRETPPAE 60

Query: 61  GQPRERIVLINPLTQGMVVIEG-TGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDD 120
              R+RI+L+NPLTQGMVVI+G + L++LF  +   K G+PPASK +IEAMP VEI E  
Sbjct: 61  ASQRQRIILVNPLTQGMVVIDGASSLEALFRELGGGKGGRPPASKESIEAMPSVEIGEGC 120

Query: 121 EERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKK 180
            + ECV+CLE  G   GV KEMPCKH+FH  CIEKWLGIHG+CPVCR++MPVEE D GKK
Sbjct: 121 GDLECVVCLEEFGVG-GVAKEMPCKHKFHSSCIEKWLGIHGNCPVCRYEMPVEERDDGKK 180

Query: 181 GEEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESG-SNDELNTGDSD 228
            EEE  G    E+WVS SF R  RR  +   ++  S  SND+ ++   D
Sbjct: 181 REEEGGG----EVWVSFSFNRSTRRGHDHDHDQPASAESNDDSSSSHGD 215

[Close]

BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
Match: RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.200e-17
Identity = 52/125 (41.60%), Postives = 69/125 (55.20%), Query Frame = 1


		  
Query: 79  VIEGTGLDSLFHGVQSI--------KDGQPPASKSAIEAMPCVEIRE--DDEERECVICL 138
           ++ G+G + L   +  I        + G PPASKSAIE++P VEI +     E  C +C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 139 EVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKGEEEESGER 194
           EV    +   +EMPCKH FHG+CI  WL I  SCPVCRF++P    D  ++  EEE    
Sbjct: 220 EVFEAGIEG-REMPCKHIFHGDCIVPWLSIRNSCPVCRFELP---SDPIQRSNEEEHAVG 279

[Close]

BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
Match: RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.000e-17
Identity = 46/117 (39.32%), Postives = 63/117 (53.85%), Query Frame = 1


		  
Query: 79  VIEGTGLDSLFHGVQSI--------KDGQPPASKSAIEAMPCVEIREDD--EERECVICL 138
           ++ G+G + L   +  I        + G PPASKSAIE++P VEI +     E  C +C 
Sbjct: 144 ILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCT 203

Query: 139 EVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKGEEEES 186
           E+        +EMPCKH FH +CI  WL I  SCPVCRF++P E        EE+ +
Sbjct: 204 EIFETETEA-REMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNA 259

[Close]

BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.300e-16
Identity = 50/136 (36.76%), Postives = 72/136 (52.94%), Query Frame = 1


		  
Query: 82  GTGLDSLFHGVQSI---KDGQPPASKSAIEAMPCVEIRED---DEERECVICLEVCGPSV 141
           G GL+ L   +      + G PPASKSAI+A+P V++ +D    E  +C +C++      
Sbjct: 168 GPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGS 227

Query: 142 GVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEED-----------GGKKGEEEE 201
            V K+MPCKH FH +C+  WL +H SCPVCRF++P ++ D           G  +G  E 
Sbjct: 228 DV-KQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEG 287

[Close]

BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.900e-16
Identity = 71/229 (31.00%), Postives = 100/229 (43.67%), Query Frame = 1


		  
Query: 28  SSFLPFLLGFPISDPTQTRSNSGENSPNHPTPEGQPR----------------------- 87
           +S LP L  FP S  T T S+S    PN+P+  G  R                       
Sbjct: 89  ASLLPLL--FPSSSSTTT-SSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLNDLR 148

Query: 88  ------ERIVLINPLTQGMVV-------IEGTGLDSLFHGVQSI---KDGQPPASKSAIE 147
                 E ++  NP  QG  +         G GL+ L   +      + G PPASKSAIE
Sbjct: 149 SSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIE 208

Query: 148 AMPCVEIREDD---EERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVC 200
           A+P V I + +   E  +C +C++         K+MPCKH +H +C+  WL +H SCPVC
Sbjct: 209 ALPLVNITKSNLNSEFNQCAVCMDDFEEGTEA-KQMPCKHLYHKDCLLPWLELHNSCPVC 268

[Close]

BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
Match: RHC2A_ARATH (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana GN=RHC2A PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.700e-14
Identity = 53/149 (35.57%), Postives = 68/149 (45.64%), Query Frame = 1


		  
Query: 70  INPLTQGMV-VIEGTGLDSLFHGVQSIKDG---------QPPASKSAIEAMPCVEIRE-- 129
           + PL   M   + G+G D L   +  I+            PPASKSAIEA+P +EI    
Sbjct: 133 LRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKSAIEALPLIEIDPTH 192

Query: 130 --DDEERECVICLE--VCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEE 189
              D +  C +C E  V   S    +EMPC H +H +CI  WL I  SCPVCR ++P E+
Sbjct: 193 LLSDSQSHCAVCKENFVLKSSA---REMPCNHIYHPDCILPWLAIRNSCPVCRHELPAED 252

Query: 190 EDGG---------KKGEEEESGERRTEIW 194
              G            EEEE       IW
Sbjct: 253 LTDGTGAALTAVTATAEEEEDSAAGLTIW 278

[Close]

BLAST of Spo10685.1 vs. TAIR (Arabidopsis)
Match: AT1G26800.1 (RING/U-box superfamily protein)

HSP 1 Score: 170.6 bits (431), Expect = 1.100e-42
Identity = 103/199 (51.76%), Postives = 131/199 (65.83%), Query Frame = 1


		  
Query: 23  RNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPEGQPRERIVLINPLTQGMVVIEG 82
           RNRDL  FLPFLLGF  SD  Q  SN  ++           RERI+L+NP TQGM+V+EG
Sbjct: 22  RNRDLYLFLPFLLGF--SD--QESSNGDDDDV------ASSRERIILVNPFTQGMIVLEG 81

Query: 83  -TGLDSLFHG-VQSIKDGQPPASKSAIEAMPCVEIREDDEERECVICLEVCGPSVGVVKE 142
            +G++ L    ++S ++G+PPASK++I+AMP VEI  D  E ECVICLE    S   VKE
Sbjct: 82  SSGMNPLLRSLLESREEGRPPASKASIDAMPIVEI--DGCEGECVICLEE-WKSEETVKE 141

Query: 143 MPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKGEEEESGERRTEIWVSVSFRR 202
           MPCKHRFHG CIEKWLG HGSCPVCR++MPV+ ++ GKK  +        EIWV  SF  
Sbjct: 142 MPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRND------GNEIWVRFSFND 198

Query: 203 GRRRTEEEAVEERESGSND 220
           GRR  +  A   ++ G++D
Sbjct: 202 GRRIRDFSA---QDGGNSD 198

[Close]

BLAST of Spo10685.1 vs. TAIR (Arabidopsis)
Match: AT1G14200.1 (RING/U-box superfamily protein)

HSP 1 Score: 113.2 bits (282), Expect = 2.100e-25
Identity = 77/207 (37.20%), Postives = 103/207 (49.76%), Query Frame = 1


		  
Query: 1   MASEPDVSAEEMQSFLERLISSRNRDLSSFLPFLLGFPISDPTQTRSNSGENSPNHPTPE 60
           M+SE D    E  S  ER+I  R   LS FLP ++     +      +  + S +  T  
Sbjct: 1   MSSENDFV--EFSSMFERIIQGRGDGLSRFLPVIVALAARE-----DDDDQGSTDQTTRR 60

Query: 61  GQPRE-RIVLINPLTQGMVVIEGTGLDSLFHGVQSIKDGQPPASKSAIEAMPCVEIREDD 120
           G P   R V+I            +GLD  F      K G+ PA KS +E MP V I ED 
Sbjct: 61  GDPLSPRFVMIG---------SRSGLDDFFS--DGGKQGRSPALKSEVENMPRVVIGEDK 120

Query: 121 EER--ECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGG 180
           E+    C ICL+       V  EMPCKH+FH +C+E+WLG H +CP+CR++MPVEE +  
Sbjct: 121 EKYGGSCAICLDEWSKG-DVAAEMPCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVE-- 178

Query: 181 KKGEEEESGERRTEIWVSVSFRRGRRR 205
              EE++ G     IW+  S   G RR
Sbjct: 181 ---EEKKIG-----IWIGFSINAGDRR 178

[Close]

BLAST of Spo10685.1 vs. TAIR (Arabidopsis)
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 91.7 bits (226), Expect = 6.700e-19
Identity = 52/125 (41.60%), Postives = 69/125 (55.20%), Query Frame = 1


		  
Query: 79  VIEGTGLDSLFHGVQSI--------KDGQPPASKSAIEAMPCVEIRE--DDEERECVICL 138
           ++ G+G + L   +  I        + G PPASKSAIE++P VEI +     E  C +C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 139 EVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKGEEEESGER 194
           EV    +   +EMPCKH FHG+CI  WL I  SCPVCRF++P    D  ++  EEE    
Sbjct: 220 EVFEAGIEG-REMPCKHIFHGDCIVPWLSIRNSCPVCRFELP---SDPIQRSNEEEHAVG 279

[Close]

BLAST of Spo10685.1 vs. TAIR (Arabidopsis)
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 90.9 bits (224), Expect = 1.100e-18
Identity = 46/117 (39.32%), Postives = 63/117 (53.85%), Query Frame = 1


		  
Query: 79  VIEGTGLDSLFHGVQSI--------KDGQPPASKSAIEAMPCVEIREDD--EERECVICL 138
           ++ G+G + L   +  I        + G PPASKSAIE++P VEI +     E  C +C 
Sbjct: 144 ILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCT 203

Query: 139 EVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEEDGGKKGEEEES 186
           E+        +EMPCKH FH +CI  WL I  SCPVCRF++P E        EE+ +
Sbjct: 204 EIFETETEA-REMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNA 259

[Close]

BLAST of Spo10685.1 vs. TAIR (Arabidopsis)
Match: AT5G56340.1 (RING/U-box superfamily protein)

HSP 1 Score: 89.7 bits (221), Expect = 2.500e-18
Identity = 74/242 (30.58%), Postives = 108/242 (44.63%), Query Frame = 1


		  
Query: 17  ERLISSRNRDLSSFLPFLLGFP--ISDPTQTRSNSGENSPNHPTPEGQPRERIVLINPLT 76
           E ++  R R   + L  L G    I+   ++  N+ +NS        + R+R+++INP  
Sbjct: 141 ESILRRRRRSSGNILQLLQGIRAGIASEYESSDNNWDNS--------RERDRVIMINPYN 200

Query: 77  QGMVVIE-----------------GTGLDSLFHGVQSI---KDGQPPASKSAIEAMPCVE 136
           Q +VV                   G GLD L   +      + G PPA K A+EA+P V+
Sbjct: 201 QSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQGTPPARKEAVEALPTVK 260

Query: 137 IREDDEERECVICLEVCGPSVGVVKEMPCKHRFHGECIEKWLGIHGSCPVCRFKMPVEEE 196
           I E     +C +CL+         KEMPCKH+FH  CI  WL +H SCPVCRF++P   +
Sbjct: 261 IMEP---LQCSVCLDDFEKGTEA-KEMPCKHKFHVRCIVPWLELHSSCPVCRFELPSSAD 320

Query: 197 DGGKKGEEEESGERRTEIWVSVSFRRGRRRTEEEAVEERESGSNDELNTGDSDSGNENEG 237
           D  +   + E                  R      V E  +G+  E N G++D G E+E 
Sbjct: 321 DDDETKTDSE------------------RVLRTRNVRETSNGNVVE-NVGNADRGREDEV 351

The following BLAST results are available for this feature:

BLAST of Spo10685.1 vs. NCBI nr
BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
BLAST of Spo10685.1 vs. TAIR (Arabidopsis)

BLAST of Spo10685.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902232948\|gb\|KNA22859.1\|	1.5e-129	100.	hypothetical protein SOVF_0296...	[more]
gi\|731343984\|ref\|XP_010683168.1\|	1.0e-61	62.0	PREDICTED: E3 ubiquitin-protei...	[more]
gi\|1009154331\|ref\|XP_015895111.1\|	3.3e-52	54.8	PREDICTED: E3 ubiquitin-protei...	[more]
gi\|819320825\|gb\|AKG50124.1\|	1.1e-50	58.4	E3 ubiquitin-protein ligase, p...	[more]
gi\|947122535\|gb\|KRH70741.1\|	4.0e-50	52.2	hypothetical protein GLYMA_02G...	[more]

BLAST of Spo10685.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RVB4_SPIOL	1.0e-129	100.	Uncharacterized protein OS=Spi...	[more]
A0A0F7G5V7_9ROSI	7.4e-51	58.4	E3 ubiquitin-protein ligase (F...	[more]
A0A0B2PS65_GLYSO	2.8e-50	52.2	E3 ubiquitin-protein ligase RI...	[more]
I1JE53_SOYBN	2.8e-50	52.2	Uncharacterized protein OS=Gly...	[more]
A0A151RS88_CAJCA	2.4e-49	51.9	E3 ubiquitin-protein ligase rn...	[more]

BLAST of Spo10685.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RDUF1_ARATH	1.2e-17	41.6	E3 ubiquitin-protein ligase RD...	[more]
RDUF2_ARATH	2.0e-17	39.3	E3 ubiquitin-protein ligase RD...	[more]
RNG1L_ARATH	1.3e-16	36.7	E3 ubiquitin-protein ligase RI...	[more]
RING1_GOSHI	2.9e-16	31.0	E3 ubiquitin-protein ligase RI...	[more]
RHC2A_ARATH	2.7e-14	35.5	Probable E3 ubiquitin-protein ...	[more]

BLAST of Spo10685.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G26800.1	1.1e-42	51.7	RING/U-box superfamily protein	[more]
AT1G14200.1	2.1e-25	37.2	RING/U-box superfamily protein	[more]
AT3G46620.1	6.7e-19	41.6	zinc finger (C3HC4-type RING f...	[more]
AT5G59550.1	1.1e-18	39.3	zinc finger (C3HC4-type RING f...	[more]
AT5G56340.1	2.5e-18	30.5	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 123..166 score: 2.0
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 124..165 score: 3.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 124..166 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10		coord: 119..173 score: 4.7
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 1..236 score: 1.6
None	No IPR available	PANTHER	PTHR22763:SF77	SUBFAMILY NOT NAMED	coord: 1..236 score: 1.6
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 118..170 score: 1.37

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding