Spo03383.1 (mRNA) | SpinachBase

Overview


Name	Spo03383.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Polynucleotidyl transferase, ribonuclease H-like superfamily protein
Location	chr3 : 93776670 .. 93777116 (-)
Sequence length	447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAATAAGGTGGTCGAGCCCGCTAATACGGTGAGTTATGCAGTTAAGGTGAGCCTTGAGGTCCGGGAGGAGCTGGAGAGAGCGAGGTCGAGGCACAATTCAGCGGTCACGTCGCTGCCCACCACTTGGTCGAAACCGGATGTTGGTTGGACGAAAGTGAATGTGGATGCGGGTGTTCTGGGAGATACTGGCACAGGTATTGGCATGGTGATGAGGGACAGTGAAGGAGTGATTGTGGCTTGCTCTTCATGGCAGGGTACGGAGAAGTGGGAAACTAGGGTGGCGGAAGCCAAGGCCGTGGCTATGGGCCTGCAGCTAGCTCAGGAGATGGGAGTAAGGGATGTGGAAATGGAAAGTGATTGTCTGATGGTAATACAACCGTTGCGTGGGGGAAATGATAAATCCAAGGAAGAATTTCACTTCTTCCAAGGAAATCACCTTTAA

mRNA sequence

ATGGAGAATAAGGTGGTCGAGCCCGCTAATACGGTGAGTTATGCAGTTAAGGTGAGCCTTGAGGTCCGGGAGGAGCTGGAGAGAGCGAGGTCGAGGCACAATTCAGCGGTCACGTCGCTGCCCACCACTTGGTCGAAACCGGATGTTGGTTGGACGAAAGTGAATGTGGATGCGGGTGTTCTGGGAGATACTGGCACAGGTATTGGCATGGTGATGAGGGACAGTGAAGGAGTGATTGTGGCTTGCTCTTCATGGCAGGGTACGGAGAAGTGGGAAACTAGGGTGGCGGAAGCCAAGGCCGTGGCTATGGGCCTGCAGCTAGCTCAGGAGATGGGAGTAAGGGATGTGGAAATGGAAAGTGATTGTCTGATGGTAATACAACCGTTGCGTGGGGGAAATGATAAATCCAAGGAAGAATTTCACTTCTTCCAAGGAAATCACCTTTAA

Coding sequence (CDS)

ATGGAGAATAAGGTGGTCGAGCCCGCTAATACGGTGAGTTATGCAGTTAAGGTGAGCCTTGAGGTCCGGGAGGAGCTGGAGAGAGCGAGGTCGAGGCACAATTCAGCGGTCACGTCGCTGCCCACCACTTGGTCGAAACCGGATGTTGGTTGGACGAAAGTGAATGTGGATGCGGGTGTTCTGGGAGATACTGGCACAGGTATTGGCATGGTGATGAGGGACAGTGAAGGAGTGATTGTGGCTTGCTCTTCATGGCAGGGTACGGAGAAGTGGGAAACTAGGGTGGCGGAAGCCAAGGCCGTGGCTATGGGCCTGCAGCTAGCTCAGGAGATGGGAGTAAGGGATGTGGAAATGGAAAGTGATTGTCTGATGGTAATACAACCGTTGCGTGGGGGAAATGATAAATCCAAGGAAGAATTTCACTTCTTCCAAGGAAATCACCTTTAA

Protein sequence

MENKVVEPANTVSYAVKVSLEVREELERARSRHNSAVTSLPTTWSKPDVGWTKVNVDAGVLGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEMESDCLMVIQPLRGGNDKSKEEFHFFQGNHL

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo03383	Spo03383	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo03383.1	Spo03383.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03383.1.CDS.1	Spo03383.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03383.1.exon.1	Spo03383.1.exon.1	exon

Homology

[Close]

BLAST of Spo03383.1 vs. NCBI nr
Match: gi|731358317|ref|XP_010690674.1| (PREDICTED: uncharacterized protein LOC104904177 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 117.1 bits (292), Expect = 2.600e-23
Identity = 67/144 (46.53%), Postives = 93/144 (64.58%), Query Frame = 1

		  
Query: 1   MENKVVEPANTVSYAVKVSLEVREELERARSRH---NSAVTSLPTTWSKPDVGWTKVNVD 60
           M N+  +P +T+ YA+ +S E  E    AR  H   +S +++ PT+W+KP   W KVNVD
Sbjct: 80  MANEKSDPVDTLGYAMMISSETLE----ARQIHVAASSEISAHPTSWAKPAPHWMKVNVD 139

Query: 61  AGVLGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVE 120
           AG++G  G GIG+V  D  G ++A S++Q  E WETRVAEAKA+  GL++A E+G   VE
Sbjct: 140 AGLVGSLGCGIGVVCWDYMGEVLAASTFQFHETWETRVAEAKAIFYGLKVALELGYAHVE 199

Query: 121 MESDCLMVIQPLRGGNDKSKEEFH 142
           +ESD L+ IQ LR G   S  +FH
Sbjct: 200 VESDSLLAIQALRSGKGGS-SDFH 218

[Close]

BLAST of Spo03383.1 vs. NCBI nr
Match: gi|731319132|ref|XP_010670097.1| (PREDICTED: uncharacterized protein LOC104887199 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 103.2 bits (256), Expect = 3.900e-19
Identity = 52/114 (45.61%), Postives = 73/114 (64.04%), Query Frame = 1

		  
Query: 25  ELERARSRHNSAVTSLPTTWSKPDVGWTKVNVDAGVLGDTGTGIGMVMRDSEGVIVACSS 84
           E  +AR + +  V   P+ WSKP  G+ KVNVDAG +G+ G G+G+V RD  G ++A  +
Sbjct: 3   EAWQARPKQDVVVAPHPSEWSKPSSGFIKVNVDAGFVGEMGCGLGVVFRDEYGEVLAAGT 62

Query: 85  WQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEMESDCLMVIQPLRGGNDKSKE 139
           +Q    WE R+AEAKA+  G++LA+EM    +E+ESD L+ IQ LR G   S E
Sbjct: 63  YQFHVAWEPRIAEAKAIFHGVKLAKEMSYSHIEVESDSLLAIQALRSGKGGSSE 116

[Close]

BLAST of Spo03383.1 vs. NCBI nr
Match: gi|731349781|ref|XP_010686173.1| (PREDICTED: uncharacterized protein LOC104900455 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 101.7 bits (252), Expect = 1.100e-18
Identity = 60/138 (43.48%), Postives = 80/138 (57.97%), Query Frame = 1

		  
Query: 1   MENKVVEPANTVSYAVKVSLEVREELERARSRHNSAVTSLPTTWSKPDVGWTKVNVDAGV 60
           ME    EP  T+SYA+K++ EV      A +     +   PT WSKP   + KVN DAG 
Sbjct: 25  MEGDQREPTETLSYALKINNEVSSASPIANTS-GVVLLPFPTGWSKPKASYVKVNTDAGQ 84

Query: 61  LGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEMES 120
           +G  G GIG+V RD  G ++A  +++   + E RVAEAKA+  G++LA E+G   VE+ES
Sbjct: 85  IGSLGCGIGVVCRDEFGEVLATGTFEHQVESEPRVAEAKAIFYGMKLALELGYSCVEVES 144

Query: 121 DCLMVIQPLRGGNDKSKE 139
           D LM IQ LR     S E
Sbjct: 145 DSLMAIQALRSDKGGSSE 161

[Close]

BLAST of Spo03383.1 vs. NCBI nr
Match: gi|731342264|ref|XP_010682311.1| (PREDICTED: uncharacterized protein LOC104897185 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 100.9 bits (250), Expect = 2.000e-18
Identity = 54/130 (41.54%), Postives = 75/130 (57.69%), Query Frame = 1

		  
Query: 1   MENKVVEPANTVSYAVKVSLEVREELERARSRHNSAVTSLPTTWSKPDVGWTKVNVDAGV 60
           ME+   +PA+T  YA+K   E ++         +  V      WS P  GW K NVDAG+
Sbjct: 144 MESNFAKPADTCYYALKTWQEAKDASLHNNPASSKMVAPHLDAWSLPQGGWVKCNVDAGM 203

Query: 61  LGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEMES 120
           + D G G+G V RD  G ++ C+  Q    WE RVAEA+AV  G++LA+ +GVR + +ES
Sbjct: 204 VADFGVGVGAVCRDEVGEVLGCAVLQQHVGWEMRVAEARAVFEGVRLAKNLGVRKLIVES 263

Query: 121 DCLMVIQPLR 131
           DCL VI  L+
Sbjct: 264 DCLQVISALK 273

[Close]

BLAST of Spo03383.1 vs. NCBI nr
Match: gi|731349717|ref|XP_010686140.1| (PREDICTED: uncharacterized protein LOC104900422 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 97.4 bits (241), Expect = 2.200e-17
Identity = 53/132 (40.15%), Postives = 82/132 (62.12%), Query Frame = 1

		  
Query: 1   MENKVVEPANTVSYAVKVSLEVREELE--RARSRHNSAVTSLPTTWSKPDVGWTKVNVDA 60
           +E +V  P  T +YA K+  E RE ++  +   +   +VTS    W  P  GW K+NVDA
Sbjct: 120 IEGEVSPPNRTAAYAAKICAEAREVVDVVQVAGKSVGSVTS-QVRWQAPSDGWMKINVDA 179

Query: 61  GVLGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEM 120
           G  G   +G+G+V RD+EG I++C++ Q    WET +AEA+AV  G+ +A+ +G++ V +
Sbjct: 180 GQAGGGLSGLGVVCRDTEGSILSCAAVQIRANWETPIAEARAVLEGVVMAKTLGLQKVII 239

Query: 121 ESDCLMVIQPLR 131
           ESDC  V+Q L+
Sbjct: 240 ESDCQRVVQALQ 250

[Close]

BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QAP7_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_206000 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.000e-11
Identity = 38/89 (42.70%), Postives = 54/89 (60.67%), Query Frame = 1

		  
Query: 44  WSKPDVGWTKVNVDAGVLGDTGTGIGMVMRDSEGVI--VACSSWQGTEKWETRVAEAKAV 103
           W  P VGW KVN DA + GD G G+G+V+ D EG    VACS  Q    W   + EAKA+
Sbjct: 117 WELPPVGWLKVNTDAAIFGDLGCGLGVVISDYEGSARRVACS--QVRHSWSVEITEAKAI 176

Query: 104 AMGLQLAQEMGVRDVEMESDCLMVIQPLR 131
            +GLQ+A +   R++ +E D  ++I+ L+
Sbjct: 177 VLGLQVANQCHERNIIVECDNSLIIKKLK 203

[Close]

BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
Match: F4NCI4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.400e-9
Identity = 48/133 (36.09%), Postives = 69/133 (51.88%), Query Frame = 1

		  
Query: 2    ENKVVEPANTVSYAVKVSLEVREELERARSRHNSAVTSLPTT---WSKPDVGWTKVNVDA 61
            E K +     V  AV+  +E  EE       H S V +L T    WS P VG  K+NVDA
Sbjct: 1170 EKKKLAFQEVVERAVRGVMEFEEECA-----HTSPVETLNTHENGWSVPPVGMVKLNVDA 1229

Query: 62   GVLGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEM 121
             V    G G+G V+RD+EG ++  +   G    +  +AEA ++  GL++A E G R++ +
Sbjct: 1230 AVFKHVGIGMGGVVRDAEGDVLLATCCGGWAMEDPAMAEACSLRYGLKVAYEAGFRNLVV 1289

Query: 122  ESDCLMVIQPLRG 132
            E DC  +   LRG
Sbjct: 1290 EMDCKKLFLQLRG 1297

[Close]

BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q8C1_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_208330 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.300e-8
Identity = 39/128 (30.47%), Postives = 62/128 (48.44%), Query Frame = 1

		  
Query: 2   ENKVVEPANTVSYAVKVSLEVREELERARSRHNSAVTSLPTTWSKPDVGWTKVNVDAGVL 61
           + +VV P    +   K+  +  E  +R      +   + P   S P  G+ KVN DA ++
Sbjct: 1   DGRVVCPTLQAASFTKLVADYIEYSQRVFKPRPNFTRTSPARLSSPSPGYIKVNTDANIV 60

Query: 62  GDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEMESD 121
            + G G+G ++RD  G  VA    +   +W    AE +AV  G+QLA  +G   V ME D
Sbjct: 61  SEAGVGLGALLRDDSGAPVAAKVKRLKARWSVEQAEVEAVKYGMQLANRLGFTKVLMECD 120

Query: 122 CLMVIQPL 130
            + V+Q +
Sbjct: 121 AVTVVQAI 128

[Close]

BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
Match: D9ZHD0_HYPPE (Retrotransposon protein OS=Hypericum perforatum GN=RT3 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.700e-8
Identity = 43/138 (31.16%), Postives = 70/138 (50.72%), Query Frame = 1

		  
Query: 10  NTVSYAVKVSLEVREELERA--------RSRHNSAVTSLPTT--WSKPDVGWTKVNVDAG 69
           N++ +     + + +ELE+          +  N  V   P T  W+ P  G+ K+NVDAG
Sbjct: 392 NSIVFRTGEEIVICKELEKGFRFWQDFMDTEGNPTVRGAPRTSKWNAPTAGFYKINVDAG 451

Query: 70  VLGDTGTGIGMVMRDSEGVIVACSSWQGTEKWETRVAEAKAVAMGLQLAQEMGVRDVEME 129
           +  + G  +G+V+RD  G  V  ++          + E +AV  GL+ A  +G+  VE+E
Sbjct: 452 LRAERGGQVGIVVRDDTGAFVMATTRSFPNLVHPTLLEGQAVYTGLEFANALGLERVELE 511

Query: 130 SDCLMVIQPL-RGGNDKS 137
           SDCL V+  L +G  D+S
Sbjct: 512 SDCLPVVMQLSKGYTDRS 529

[Close]

BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
Match: A2Q2Z8_MEDTR (Polynucleotidyl transferase, Ribonuclease H fold OS=Medicago truncatula GN=MtrDRAFT_AC153128g17v2 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.400e-8
Identity = 40/121 (33.06%), Postives = 62/121 (51.24%), Query Frame = 1

		  
Query: 30  RSRHNSAVTSLPTTWSKPDVGWTKVNVDAGVLGDTGTGIGMVMRDSEGVIVACSSWQGTE 89
           RS+H  A     T W +P++G  KVN DA +      G+G+++RD  G+++A S+W+   
Sbjct: 33  RSQHRPAKN---TKWKRPNLGLVKVNTDANLQNHGKWGLGIIIRDEVGLVMAASTWETDG 92

Query: 90  KWETRVAEAKAVAMGLQLAQEMGVRDVEMESD----CLMVIQPLRGGNDKSKEEFHFFQG 147
                 AEA A+  G++ A++ G R V  E D      M I   + G D  + +F F  G
Sbjct: 93  NDRALEAEAYALLTGMRFAKDCGFRKVVFEGDNEKLMKMEIHKAKSGFDICQFQFTFRSG 150

The following BLAST results are available for this feature:

BLAST of Spo03383.1 vs. NCBI nr
BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
BLAST of Spo03383.1 vs. ExPASy Swiss-Prot
BLAST of Spo03383.1 vs. TAIR (Arabidopsis)

BLAST of Spo03383.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
gi\|731358317\|ref\|XP_010690674.1\|	2.6e-23	46.5	PREDICTED: uncharacterized pro...	[more]
gi\|731319132\|ref\|XP_010670097.1\|	3.9e-19	45.6	PREDICTED: uncharacterized pro...	[more]
gi\|731349781\|ref\|XP_010686173.1\|	1.1e-18	43.4	PREDICTED: uncharacterized pro...	[more]
gi\|731342264\|ref\|XP_010682311.1\|	2.0e-18	41.5	PREDICTED: uncharacterized pro...	[more]
gi\|731349717\|ref\|XP_010686140.1\|	2.2e-17	40.1	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo03383.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
A0A0K9QAP7_SPIOL	8.0e-11	42.7	Uncharacterized protein (Fragm...	[more]
F4NCI4_BETVU	4.4e-9	36.0	Uncharacterized protein OS=Bet...	[more]
A0A0K9Q8C1_SPIOL	1.3e-8	30.4	Uncharacterized protein (Fragm...	[more]
D9ZHD0_HYPPE	1.7e-8	31.1	Retrotransposon protein OS=Hyp...	[more]
A2Q2Z8_MEDTR	6.4e-8	33.0	Polynucleotidyl transferase, R...	[more]

BLAST of Spo03383.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description

BLAST of Spo03383.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012337	Ribonuclease H-like domain	GENE3D	3.30.420.10		coord: 46..138 score: 1.
IPR012337	Ribonuclease H-like domain	unknown	SSF53098	Ribonuclease H-like	coord: 50..137 score: 1.0
None	No IPR available	PANTHER	PTHR33033	FAMILY NOT NAMED	coord: 14..131 score: 7.3
None	No IPR available	PFAM	PF13456	RVT_3	coord: 55..138 score: 1.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding