Spo27282.1 (mRNA)

Overview
NameSpo27282.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
Description5-formyltetrahydrofolate cyclo-ligase (6.3.3.2)
Locationchr5 : 8673548 .. 8679337 (+)
Sequence length1101
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTGACCAGACTTTAACCAAACTCGTGCGCGACTAGTTCACGAATATACCATCTCACTTTTATTCTTACAAATCTACCGTAGACATCTCTACTCTCTAGGGCCTAAAATAGTGTAGTAAATGGGTATAGGGCTTAAGACTTAAAGATAATGAACACATTTGGGAGACTTTCAAGGCAAAAGGCCAAAGAAGCAAAGAAACTTATGACACTGTCCTTTTCCCTCCACCATCCGTGCACGTTAGCATCGCCACCCTTTTCCACCACCAATTTCCGCTTTCTTAACCCCCGCCAAAACTGTCCGCCACCTCACTCCGCCTCTCTCTCCTCCCTCAGGATGAGCTCCACCACTGTTGACGGTAGCAATATTAACAGTCCCGGTGATGTTGCCGCTACTGAGTTGGACCACATTTTCAAGCAGAAACGCACCATTCGATCCAAAGTCCGCAAATCCCTTAAGGAGATGGACCCTTCTCAGCGCGCAATTGAAGGTATAAGAAAATTTCTTTCTTCAATTCTAGTACTCCATTATTTTGATTATATTTGTGAACTGGGGTTTTGCCGTTTTGGCAGCATTGCTTAATTTGTTGGTATTTTGATTAAATTGGATAATTTGAGTAGATTTGTATTTTGATTTGATTAGATTAGCAATTTGAATAGATTGGATGAATTGAGTGGAGTAAACATTTGGGCAGCAATTGGGCATTTTGGTAGTTTGATCAAGTAGGTATTCAAATGAGGATGGAACTTGCCCTTGTTTCCTGAATGTTGGATCAAATTGTGAAGTGCCTTGTGTAGAGGTAATAATTTGATCTTGAAATAGTGAAAGAATGGGGACATTGATGATTCAAGTACTCCCTTCTTCCAGACCTGTTGTCTGGACTTTCTTAAGGTGAAAATGGGATAAATTAAGCTTTGAAACAGGTAGCCATTTTTCCCTTACATCCATTACTCATAAGTTATAGAGTTTGAAACAAAATGCTGCATACAATCTGCTTTATACTAATCAATACAGTAATTGCGGGGAAAGGGAAAAGGGACTAGTAAAGTTCGTGCAATCTGCAAAAGTCCTACACTTTGTTACCAACTTGTCAGCAAGCTGGGCTCCTCCTCTTTTGACAAAGATCATGCACATATTATGGAGTTATTGGCATGAGAACCTTGCAATCAGACGCCACATGAAATAGTAATCGCTACTTAACATAGGTTTTTGAAGCAGCTGAATAGCATCAGAACAATCCGAGCAGAGGATCAAGTCATCAATATTGAGACTAATAGCTAATTAGAGCCCCTCTTGTATACTATTCACCGCTGCTAGCAAAGGATCATGTCTGTGAAAATCTTGAATCCAATTCCTCTTCAATATCTGAAAACACCCGCTGATCCCAACATGAGATCTACTATTTATAAAGGATGCAGCCACTTGTAACATGACCATGTACTATTATGAACAATTCCACTACGAGAAAAAATGTCTGTAGATAAGGTTGGAATTTGTCCTAATGTGATTAGTGGAGATGAAATCCCAGCTGTTAGAAGAACCTAAGTGCAGAATAAAACTCTGGTTTCACTTGAAAGTTGGACTCAGTTTTTTATTTTCTGTACCTTTCCAGATTTCAAAAGCAAGGAAGGGAATATGCTCCAGTTAATTCCCTCCACCTAAAGATTCCCAGTCAAATTCATCCTTAACCAACCACGTGATGCAATCACCTTGGAGGAGGGAAGGCGGAATTGGAAGTGCGGTCCAAGTGACATGGGAATGGGCAAAGTCAGCGATAGCATGAAGGGAGTCTTCCAAGACGAGAAAAAATGGAGATTATGATCGGTTACGATTAAGCGATAAAAAGGAGACTTATGAGGTAGCCTGTTTATCCTTAGAAGCTAGAAGAAAAAAAATGAATTTTGAGCTTAACTTTTGCCTTCCTTAACCGGACAAGGTCATCCACAAGCTCTTTCCCTCCAATATCCTTATCATAGGCAAGACCTATGGAGAAACCCTTATAAGAATGAAAACAAGAGCGTGGGTATCTGGTTGAGGGGATATGCTTTTAATTCGACTAATAAGATCATGGTGGAGGTCGTACGGGGAGGGTAAGGTCCCATTGTCCATTAAGGACGAAGTCCTTAAGTGGTCTATCAACTTCACCTTGCTCGTGGGGACCCTGAATCATTTGTCGTGAAGGGCCTATCTCAAGCCGATATTCTTTCCTAAAAGATATTGCTTCCCCATTCCCAGAACCAAAGTATTGTTAGTAGAAAGCAAATCCCAATCCTTACAAAACTTTCCACATCGGAGACCTGTAGACGAACTTTGTGACATGAGTTTGTGAACATACTTGGGGAGTTGGGGGTGATGCATTCTTGTGCTAGGTTACTATCCCCTTGAGATAACCTCCATCGTAGCTCATGTGGGAATTGTTGATGGAGATTTGACTTCTTATTCCTAGTCCCCACTACATGCTTTGGTTTGAAGTTTTGAAAGTCATTTCCCTTGATACCATCAGTTTTTTGTGGCTTCCCTCCTCATCCGCCCATAGGAAGTTGTTGCTAATCCTATCTAGCTCCTTCGAAGTAGCAATTGGGAGAAAAGACGTTTGCATATAGTAGAGGAATGGTGAACAGGGTGTAATTATTTAGGCACAAGCAACCCCTTAGGAGAGGAACTTAGTTTTATAATACTCTAGGAGGTTTCTTACTTTGTCCATGGCAAATTGAACTTGAGCTATAGTGGACCTTTTGTGGGAGACATGCAACCATGGTCCAACCCTAGGTTATCTGACTCATGGATGCTGAGAGTATTTTGGAACAGAACACGTTGACCTTCAACAGTGTTATTTGAGAAAGTTAGACGGCTTTTCCTCTCATATTATCTTTTAGGATAATATATTTATATAAAGTTCTCAAATATATTATTATCCTCGTGTACAACACAAACTTGAATCTCATTGATCACTGCAACAATTCTTGAATTTTACCTATAACCTGCAAAACGTGGACATTCAATAGTCTGGTAATGTTAAACTCATTGTATTTTTACAGGAAAAATAATTGAATTTCTGGTGGACTGTTCCATTCTGATATAATTGACGTATTTTAAGACAAAAAATAGCTGAAGTGGTGGAGCTTGATAACAGCATATTCCACCACTATTTGCTTGTGATCTGCCGGCTGCCTTCTCTGAATTCCACAACTATAGAATGTCATCATTCTAATAGGTCACTGTTACCTTACTTATGTAACTTGTATCATTTTCTTTCTATTCAGATGATACAATTCAGCGTTTGGTTTTGGATGCTCCATGGTTTAAATCAAGCAGAAGACTATGTGCATATATAAGCTGCCGTGCCTTACGAGAAGTTGATACATCTAAAGTTTTGGCAGATACCCTACAGACTTCAGACAAAGGTTTGACTCAGTGCCTGAGCGTATGAATCTTTATTTCAAGGGTGCTAGTCTATGGGCAGTAAAATGATTTTTAACTTAGGTCACTGATGTATAGTTAATCAAGAAAGTTCTCTTGCATTAGATCAACTTGGTCTGTCTTATTAAACCTTGAAGTAACAACAGTCTTAAAATTCACCATTGGCATCAATGAAGATTACTAATGTGGAACTTTAACATGGGAGTTTATCATTTATTGGTGAATCTGCCCCTCTAGGCTTTGCCGAAGCTAGTAAAAAAAGTTTCCCGTATGGGAATTTGGGTTGACAACCTTCTTGAAAGAACTGGTCATGTACCTTCCTTTTCTTTAATTGTAAACTTTTAGTTTTGGCTTGCAGACAAACAGGATTTCGAAGAGAAAACCCTTTATGTGCCAAGGGTGGAGGACAAGAACAGTTATATGCGGATGCTTAACATCTCAAGCATGGATGATTTAATCGCAAACTCAATGGACATCCTGGAACCTGCTCCAGTAGATAGTGGAGGGAACCAACGTGAAAATGGTAGCTCATATTTCTAGTCAATTTTTCTGTATGGCATATGTTGTTTAGGTGACTTCTTTTTCGCAGTTAACAGAATGTGCTTATTGAATTTTTCTCATGAACCTTACTGAAACTTTGCTTACTAGACAGTAGACACGCTAGGGGTTTAACTGCTACTCGGTATCTTTTCGACAGTCCTGTATCGGAAATGTGTAAAATTGGTCTCTTATCACAACATAATTGTCCCTCTTGCTCATTTGTGTCAGCACTCAGTCCAAGTATGTTGTCACATTTACTTGGTTTCCTCATGTCTCAACTCCACTCTTTACTAAATATTCTGCTTTAAACTTGGTAGCAAGAAGTCAAATTTCCATTTATTGCATGTTTGGTTTATTTGAATAGGATTAATCGAGGGTTTTTCAAGTTATAGAATGTTGTAAAGGGGCAGGTGTCTGTATATTGTGTAGTATAAGTATGTCCGTTAAAAATTTTCTTTTTGTTTGGCTGTAGTTAGTAGACCATTTAGCATCCTACTTCTTGATTTTCTTGGATATGAATCTTGTTATGATGATTCGTCTCTTGTTGCAGTTATGCATGCTACTGAAGCAGTTGATCTCTTGCTCTTACCTGGTAATATAGCTTATCACCTGCTCAGTTCATGTCCACATTGCTGAAATAGAAAGTGTTATCTTTAGGTTGATTCATATTATCCCCTTCTCCAGGACTTGCGTTTGACAAATCTGGTAGACGCTTAGGCCGTGGCGGAGGGTATGTTCAAAAACTTTTAAGTCCTTACAGTTATATTTCTTGATTGGTGTATATTACAAATCAAATGACTGGGCATGGTTCCATAAGGTGTATCCCTCCATCACATGTCTGTCTGAGTATGCTCGCCTGTTAAACGTTCTTCCCCCTTCCTTACCTATTAAGGGAAAATATAAATAGAAAACAAACTAGCAGGTCTGTCTCTGCATAGCATGTATCAAGTTTAAAAGGCATTGGTTATCACCCAAAAAAAATAAAATTGCCCTATCTTTAAGTGGTGTTGTTGCTTTACTGATCTCCTAATCTTATATTTTGAAACATTTTGAAGTTACTATGACACTTTCCTGAGGAACTATCGACAGCTTGCTAACGAGAAAAACTGGAAACAACCACTGCTTGGTATGTTTTACATCCCTCCCCATATCAGTTATCTGTTTTTTTTTGGGACCAAACTCCCCATATCAGAATTATTAAAGATGGCCGGGCTCACGGGCTGGGGGCTAAACGTGGCGGGCCCATACCAAGCACACCTCTGTGACGGGCCGGGCTGGGTCGAAAAGTTCAAAACATGCCAATGGGTGTCGTGTTGGACCAGACCAGGCCGGGTTGTCGTGACTAAGCCTAAATTTACTGTATTTAGCGTGTTTTGTCGTGCCAGGTCGAGCCATGTCGTGCTTTTTCCGAAAAAAAATGTGGGTCAGGCCCAGCCCACTACTTCGTGCTCGTTCCGGACCGACGGGCCGTGCTTTTTTCGTGCCGGGTCGGGTTGGGTCTTGGACCGGCCTGGCCCACAGCCATCTTTAATATTATCAGTTATCTTATTTGTCAGAGATTTGGGTAAAATCAGATTTATTGATTGATTAATCTTGTTGTGTAGTTGCTCTGTCATATTCTGTACAAATCTTGGACGATGGAGTGATTCCAATCACCCCCAATGATGTCTTTGTGGATGCTTTAGTATCCCCAGCTGGTGTTATTCCCATAAACCCAGCTGCAATTCAAAGGTATCTGATTTAAAACTATCGCCACATTGCTGCTTCTTTATGGTAGCTATACATATATATAATTTTGTCCAATGTTTCAGGATGGAGCATGGAATATGA

mRNA sequence

CTTTTGACCAGACTTTAACCAAACTCGTGCGCGACTAGTTCACGAATATACCATCTCACTTTTATTCTTACAAATCTACCGTAGACATCTCTACTCTCTAGGGCCTAAAATAGTGTAGTAAATGGGTATAGGGCTTAAGACTTAAAGATAATGAACACATTTGGGAGACTTTCAAGGCAAAAGGCCAAAGAAGCAAAGAAACTTATGACACTGTCCTTTTCCCTCCACCATCCGTGCACGTTAGCATCGCCACCCTTTTCCACCACCAATTTCCGCTTTCTTAACCCCCGCCAAAACTGTCCGCCACCTCACTCCGCCTCTCTCTCCTCCCTCAGGATGAGCTCCACCACTGTTGACGGTAGCAATATTAACAGTCCCGGTGATGTTGCCGCTACTGAGTTGGACCACATTTTCAAGCAGAAACGCACCATTCGATCCAAAGTCCGCAAATCCCTTAAGGAGATGGACCCTTCTCAGCGCGCAATTGAAGATGATACAATTCAGCGTTTGGTTTTGGATGCTCCATGGTTTAAATCAAGCAGAAGACTATGTGCATATATAAGCTGCCGTGCCTTACGAGAAGTTGATACATCTAAAGTTTTGGCAGATACCCTACAGACTTCAGACAAAGACAAACAGGATTTCGAAGAGAAAACCCTTTATGTGCCAAGGGTGGAGGACAAGAACAGTTATATGCGGATGCTTAACATCTCAAGCATGGATGATTTAATCGCAAACTCAATGGACATCCTGGAACCTGCTCCAGTAGATAGTGGAGGGAACCAACGTGAAAATGTTATGCATGCTACTGAAGCAGTTGATCTCTTGCTCTTACCTGGACTTGCGTTTGACAAATCTGGTAGACGCTTAGGCCGTGGCGGAGGTTACTATGACACTTTCCTGAGGAACTATCGACAGCTTGCTAACGAGAAAAACTGGAAACAACCACTGCTTGTTGCTCTGTCATATTCTGTACAAATCTTGGACGATGGAGTGATTCCAATCACCCCCAATGATGTCTTTGTGGATGCTTTAGTATCCCCAGCTGGTGTTATTCCCATAAACCCAGCTGCAATTCAAAGGATGGAGCATGGAATATGA

Coding sequence (CDS)

ATGAACACATTTGGGAGACTTTCAAGGCAAAAGGCCAAAGAAGCAAAGAAACTTATGACACTGTCCTTTTCCCTCCACCATCCGTGCACGTTAGCATCGCCACCCTTTTCCACCACCAATTTCCGCTTTCTTAACCCCCGCCAAAACTGTCCGCCACCTCACTCCGCCTCTCTCTCCTCCCTCAGGATGAGCTCCACCACTGTTGACGGTAGCAATATTAACAGTCCCGGTGATGTTGCCGCTACTGAGTTGGACCACATTTTCAAGCAGAAACGCACCATTCGATCCAAAGTCCGCAAATCCCTTAAGGAGATGGACCCTTCTCAGCGCGCAATTGAAGATGATACAATTCAGCGTTTGGTTTTGGATGCTCCATGGTTTAAATCAAGCAGAAGACTATGTGCATATATAAGCTGCCGTGCCTTACGAGAAGTTGATACATCTAAAGTTTTGGCAGATACCCTACAGACTTCAGACAAAGACAAACAGGATTTCGAAGAGAAAACCCTTTATGTGCCAAGGGTGGAGGACAAGAACAGTTATATGCGGATGCTTAACATCTCAAGCATGGATGATTTAATCGCAAACTCAATGGACATCCTGGAACCTGCTCCAGTAGATAGTGGAGGGAACCAACGTGAAAATGTTATGCATGCTACTGAAGCAGTTGATCTCTTGCTCTTACCTGGACTTGCGTTTGACAAATCTGGTAGACGCTTAGGCCGTGGCGGAGGTTACTATGACACTTTCCTGAGGAACTATCGACAGCTTGCTAACGAGAAAAACTGGAAACAACCACTGCTTGTTGCTCTGTCATATTCTGTACAAATCTTGGACGATGGAGTGATTCCAATCACCCCCAATGATGTCTTTGTGGATGCTTTAGTATCCCCAGCTGGTGTTATTCCCATAAACCCAGCTGCAATTCAAAGGATGGAGCATGGAATATGA

Protein sequence

MNTFGRLSRQKAKEAKKLMTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo27282Spo27282gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo27282.1Spo27282.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo27282.1.exon.1Spo27282.1.exon.1exon
Spo27282.1.exon.2Spo27282.1.exon.2exon
Spo27282.1.exon.3Spo27282.1.exon.3exon
Spo27282.1.exon.4Spo27282.1.exon.4exon
Spo27282.1.exon.5Spo27282.1.exon.5exon
Spo27282.1.exon.6Spo27282.1.exon.6exon
Spo27282.1.exon.7Spo27282.1.exon.7exon
Spo27282.1.exon.8Spo27282.1.exon.8exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo27282.1.utr5p.1Spo27282.1.utr5p.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo27282.1.CDS.1Spo27282.1.CDS.1CDS
Spo27282.1.CDS.2Spo27282.1.CDS.2CDS
Spo27282.1.CDS.3Spo27282.1.CDS.3CDS
Spo27282.1.CDS.4Spo27282.1.CDS.4CDS
Spo27282.1.CDS.5Spo27282.1.CDS.5CDS
Spo27282.1.CDS.6Spo27282.1.CDS.6CDS
Spo27282.1.CDS.7Spo27282.1.CDS.7CDS
Spo27282.1.CDS.8Spo27282.1.CDS.8CDS


Homology
[Close]
BLAST of Spo27282.1 vs. NCBI nr
Match: gi|902204494|gb|KNA14888.1| (hypothetical protein SOVF_103230 [Spinacia oleracea])

HSP 1 Score: 588.2 bits (1515), Expect = 8.600e-165
Identity = 297/298 (99.66%), Postives = 298/298 (100.00%), Query Frame = 1 

		  

Query: 19  MTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGD 78
           MTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGD
Sbjct: 1   MTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGD 60

Query: 79  VAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYIS 138
           VAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYIS
Sbjct: 61  VAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYIS 120

Query: 139 CRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSM 198
           CRALREVDTSKVLADTLQTSDKDKQ+FEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSM
Sbjct: 121 CRALREVDTSKVLADTLQTSDKDKQNFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSM 180

Query: 199 DILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLA 258
           DILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLA
Sbjct: 181 DILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLA 240

Query: 259 NEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI 317
           NEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI
Sbjct: 241 NEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI 298
[Close]
BLAST of Spo27282.1 vs. NCBI nr
Match: gi|731335271|ref|XP_010678643.1| (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 493.8 bits (1270), Expect = 2.200e-136
Identity = 262/317 (82.65%), Postives = 283/317 (89.27%), Query Frame = 1 

		  

Query: 1   MNTFGRLSRQKAKEAKKLMTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSS 60
           MNTFGRLS QKAKE KKLMTLSFSLHH CTLASPP ST N RFLN R+  PP    SLSS
Sbjct: 1   MNTFGRLSSQKAKEVKKLMTLSFSLHHLCTLASPPPST-NSRFLNVRR--PPLFFNSLSS 60

Query: 61  LRMSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRL 120
           LRMSSTT++G+NI++  DV  TEL+ IFKQKR IRSKVRKSLKEMDPSQRA+EDD +QRL
Sbjct: 61  LRMSSTTIEGTNISNSTDVTDTELESIFKQKRVIRSKVRKSLKEMDPSQRALEDDALQRL 120

Query: 121 VLDAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDK-DKQDFEEKTLYVPRVEDKN 180
           VLDAPWFKSSRRLCAYISCRALREVDT+ +LA++LQ S K DKQD   KTLYVPRVEDKN
Sbjct: 121 VLDAPWFKSSRRLCAYISCRALREVDTTTILAESLQISGKGDKQDLGGKTLYVPRVEDKN 180

Query: 181 SYMRMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRR 240
           S+MRMLNI SMDDLIANSM+ILEPAPVDS GNQRE+VMHA+EAVDLLLLPGLAFDKSGRR
Sbjct: 181 SHMRMLNICSMDDLIANSMEILEPAPVDSRGNQREDVMHASEAVDLLLLPGLAFDKSGRR 240

Query: 241 LGRGGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPA 300
           LGRGGGYYDTFLRNYRQL N++NWK PLLVALSYSVQILDDGVIPITPNDVFVDAL+SPA
Sbjct: 241 LGRGGGYYDTFLRNYRQLVNKQNWKLPLLVALSYSVQILDDGVIPITPNDVFVDALISPA 300

Query: 301 GVIPINPAAIQRMEHGI 317
           GVIPI+ AA QRMEH I
Sbjct: 301 GVIPISSAARQRMEHDI 314
[Close]
BLAST of Spo27282.1 vs. NCBI nr
Match: gi|731335273|ref|XP_010678644.1| (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 492.7 bits (1267), Expect = 4.900e-136
Identity = 261/316 (82.59%), Postives = 282/316 (89.24%), Query Frame = 1 

		  

Query: 1   MNTFGRLSRQKAKEAKKLMTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSS 60
           MNTFGRLS QKAKE KKLMTLSFSLHH CTLASPP ST N RFLN R+  PP    SLSS
Sbjct: 1   MNTFGRLSSQKAKEVKKLMTLSFSLHHLCTLASPPPST-NSRFLNVRR--PPLFFNSLSS 60

Query: 61  LRMSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRL 120
           LRMSSTT++G+NI++  DV  TEL+ IFKQKR IRSKVRKSLKEMDPSQRA+EDD +QRL
Sbjct: 61  LRMSSTTIEGTNISNSTDVTDTELESIFKQKRVIRSKVRKSLKEMDPSQRALEDDALQRL 120

Query: 121 VLDAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNS 180
           VLDAPWFKSSRRLCAYISCRALREVDT+ +LA++LQ S  DKQD   KTLYVPRVEDKNS
Sbjct: 121 VLDAPWFKSSRRLCAYISCRALREVDTTTILAESLQIS--DKQDLGGKTLYVPRVEDKNS 180

Query: 181 YMRMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRL 240
           +MRMLNI SMDDLIANSM+ILEPAPVDS GNQRE+VMHA+EAVDLLLLPGLAFDKSGRRL
Sbjct: 181 HMRMLNICSMDDLIANSMEILEPAPVDSRGNQREDVMHASEAVDLLLLPGLAFDKSGRRL 240

Query: 241 GRGGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAG 300
           GRGGGYYDTFLRNYRQL N++NWK PLLVALSYSVQILDDGVIPITPNDVFVDAL+SPAG
Sbjct: 241 GRGGGYYDTFLRNYRQLVNKQNWKLPLLVALSYSVQILDDGVIPITPNDVFVDALISPAG 300

Query: 301 VIPINPAAIQRMEHGI 317
           VIPI+ AA QRMEH I
Sbjct: 301 VIPISSAARQRMEHDI 311
[Close]
BLAST of Spo27282.1 vs. NCBI nr
Match: gi|870858996|gb|KMT10460.1| (hypothetical protein BVRB_5g115860 isoform B [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 418.7 bits (1075), Expect = 9.100e-114
Identity = 214/255 (83.92%), Postives = 234/255 (91.76%), Query Frame = 1 

		  

Query: 63  MSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVL 122
           MSSTT++G+NI++  DV  TEL+ IFKQKR IRSKVRKSLKEMDPSQRA+EDD +QRLVL
Sbjct: 1   MSSTTIEGTNISNSTDVTDTELESIFKQKRVIRSKVRKSLKEMDPSQRALEDDALQRLVL 60

Query: 123 DAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDK-DKQDFEEKTLYVPRVEDKNSY 182
           DAPWFKSSRRLCAYISCRALREVDT+ +LA++LQ S K DKQD   KTLYVPRVEDKNS+
Sbjct: 61  DAPWFKSSRRLCAYISCRALREVDTTTILAESLQISGKGDKQDLGGKTLYVPRVEDKNSH 120

Query: 183 MRMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLG 242
           MRMLNI SMDDLIANSM+ILEPAPVDS GNQRE+VMHA+EAVDLLLLPGLAFDKSGRRLG
Sbjct: 121 MRMLNICSMDDLIANSMEILEPAPVDSRGNQREDVMHASEAVDLLLLPGLAFDKSGRRLG 180

Query: 243 RGGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGV 302
           RGGGYYDTFLRNYRQL N++NWK PLLVALSYSVQILDDGVIPITPNDVFVDAL+SPAGV
Sbjct: 181 RGGGYYDTFLRNYRQLVNKQNWKLPLLVALSYSVQILDDGVIPITPNDVFVDALISPAGV 240

Query: 303 IPINPAAIQRMEHGI 317
           IPI+ AA QRMEH I
Sbjct: 241 IPISSAARQRMEHDI 255
[Close]
BLAST of Spo27282.1 vs. NCBI nr
Match: gi|870858995|gb|KMT10459.1| (hypothetical protein BVRB_5g115860 isoform A [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 417.2 bits (1071), Expect = 2.600e-113
Identity = 213/254 (83.86%), Postives = 233/254 (91.73%), Query Frame = 1 

		  

Query: 63  MSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVL 122
           MSSTT++G+NI++  DV  TEL+ IFKQKR IRSKVRKSLKEMDPSQRA+EDD +QRLVL
Sbjct: 1   MSSTTIEGTNISNSTDVTDTELESIFKQKRVIRSKVRKSLKEMDPSQRALEDDALQRLVL 60

Query: 123 DAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYM 182
           DAPWFKSSRRLCAYISCRALREVDT+ +LA++LQ SDK  QD   KTLYVPRVEDKNS+M
Sbjct: 61  DAPWFKSSRRLCAYISCRALREVDTTTILAESLQISDK--QDLGGKTLYVPRVEDKNSHM 120

Query: 183 RMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGR 242
           RMLNI SMDDLIANSM+ILEPAPVDS GNQRE+VMHA+EAVDLLLLPGLAFDKSGRRLGR
Sbjct: 121 RMLNICSMDDLIANSMEILEPAPVDSRGNQREDVMHASEAVDLLLLPGLAFDKSGRRLGR 180

Query: 243 GGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVI 302
           GGGYYDTFLRNYRQL N++NWK PLLVALSYSVQILDDGVIPITPNDVFVDAL+SPAGVI
Sbjct: 181 GGGYYDTFLRNYRQLVNKQNWKLPLLVALSYSVQILDDGVIPITPNDVFVDALISPAGVI 240

Query: 303 PINPAAIQRMEHGI 317
           PI+ AA QRMEH I
Sbjct: 241 PISSAARQRMEHDI 252
[Close]
BLAST of Spo27282.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R5U6_SPIOL (5-formyltetrahydrofolate cyclo-ligase OS=Spinacia oleracea GN=SOVF_103230 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 6.000e-165
Identity = 297/298 (99.66%), Postives = 298/298 (100.00%), Query Frame = 1 

		  

Query: 19  MTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGD 78
           MTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGD
Sbjct: 1   MTLSFSLHHPCTLASPPFSTTNFRFLNPRQNCPPPHSASLSSLRMSSTTVDGSNINSPGD 60

Query: 79  VAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYIS 138
           VAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYIS
Sbjct: 61  VAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYIS 120

Query: 139 CRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSM 198
           CRALREVDTSKVLADTLQTSDKDKQ+FEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSM
Sbjct: 121 CRALREVDTSKVLADTLQTSDKDKQNFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSM 180

Query: 199 DILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLA 258
           DILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLA
Sbjct: 181 DILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLA 240

Query: 259 NEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI 317
           NEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI
Sbjct: 241 NEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRMEHGI 298
[Close]
BLAST of Spo27282.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C9Q0_BETVU (5-formyltetrahydrofolate cyclo-ligase OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g115860 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.300e-114
Identity = 214/255 (83.92%), Postives = 234/255 (91.76%), Query Frame = 1 

		  

Query: 63  MSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVL 122
           MSSTT++G+NI++  DV  TEL+ IFKQKR IRSKVRKSLKEMDPSQRA+EDD +QRLVL
Sbjct: 1   MSSTTIEGTNISNSTDVTDTELESIFKQKRVIRSKVRKSLKEMDPSQRALEDDALQRLVL 60

Query: 123 DAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDK-DKQDFEEKTLYVPRVEDKNSY 182
           DAPWFKSSRRLCAYISCRALREVDT+ +LA++LQ S K DKQD   KTLYVPRVEDKNS+
Sbjct: 61  DAPWFKSSRRLCAYISCRALREVDTTTILAESLQISGKGDKQDLGGKTLYVPRVEDKNSH 120

Query: 183 MRMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLG 242
           MRMLNI SMDDLIANSM+ILEPAPVDS GNQRE+VMHA+EAVDLLLLPGLAFDKSGRRLG
Sbjct: 121 MRMLNICSMDDLIANSMEILEPAPVDSRGNQREDVMHASEAVDLLLLPGLAFDKSGRRLG 180

Query: 243 RGGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGV 302
           RGGGYYDTFLRNYRQL N++NWK PLLVALSYSVQILDDGVIPITPNDVFVDAL+SPAGV
Sbjct: 181 RGGGYYDTFLRNYRQLVNKQNWKLPLLVALSYSVQILDDGVIPITPNDVFVDALISPAGV 240

Query: 303 IPINPAAIQRMEHGI 317
           IPI+ AA QRMEH I
Sbjct: 241 IPISSAARQRMEHDI 255
[Close]
BLAST of Spo27282.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CE54_BETVU (5-formyltetrahydrofolate cyclo-ligase OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g115860 PE=3 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.800e-113
Identity = 213/254 (83.86%), Postives = 233/254 (91.73%), Query Frame = 1 

		  

Query: 63  MSSTTVDGSNINSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVL 122
           MSSTT++G+NI++  DV  TEL+ IFKQKR IRSKVRKSLKEMDPSQRA+EDD +QRLVL
Sbjct: 1   MSSTTIEGTNISNSTDVTDTELESIFKQKRVIRSKVRKSLKEMDPSQRALEDDALQRLVL 60

Query: 123 DAPWFKSSRRLCAYISCRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYM 182
           DAPWFKSSRRLCAYISCRALREVDT+ +LA++LQ SDK  QD   KTLYVPRVEDKNS+M
Sbjct: 61  DAPWFKSSRRLCAYISCRALREVDTTTILAESLQISDK--QDLGGKTLYVPRVEDKNSHM 120

Query: 183 RMLNISSMDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGR 242
           RMLNI SMDDLIANSM+ILEPAPVDS GNQRE+VMHA+EAVDLLLLPGLAFDKSGRRLGR
Sbjct: 121 RMLNICSMDDLIANSMEILEPAPVDSRGNQREDVMHASEAVDLLLLPGLAFDKSGRRLGR 180

Query: 243 GGGYYDTFLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVI 302
           GGGYYDTFLRNYRQL N++NWK PLLVALSYSVQILDDGVIPITPNDVFVDAL+SPAGVI
Sbjct: 181 GGGYYDTFLRNYRQLVNKQNWKLPLLVALSYSVQILDDGVIPITPNDVFVDALISPAGVI 240

Query: 303 PINPAAIQRMEHGI 317
           PI+ AA QRMEH I
Sbjct: 241 PISSAARQRMEHDI 252
[Close]
BLAST of Spo27282.1 vs. UniProtKB/TrEMBL
Match: B9I331_POPTR (5-formyltetrahydrofolate cyclo-ligase OS=Populus trichocarpa GN=POPTR_0012s07480g PE=3 SV=2)

HSP 1 Score: 345.1 bits (884), Expect = 8.900e-92
Identity = 177/244 (72.54%), Postives = 205/244 (84.02%), Query Frame = 1 

		  

Query: 71  SNINSPGDVA-ATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKS 130
           +++ SPG ++ AT LD IFKQK+ +RSKVRKSLK MDPS R+ EDD IQ LVL+APWFKS
Sbjct: 46  ASLCSPGTMSTATNLDGIFKQKQVLRSKVRKSLKSMDPSLRSQEDDAIQSLVLEAPWFKS 105

Query: 131 SRRLCAYISCRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISS 190
           S+RLCAYI+C ALREVDTSK+L+  LQ   KD      K LYVPRVEDKNS+MRMLNISS
Sbjct: 106 SQRLCAYITCSALREVDTSKLLSQILQNPTKDGSVENAKKLYVPRVEDKNSHMRMLNISS 165

Query: 191 MDDLIANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDT 250
           MDDLIANSMDILEPAPVD  GN RE+VM A + VDL LLPGLAFDKSGRRLGRGGGYYDT
Sbjct: 166 MDDLIANSMDILEPAPVDQHGNAREDVMQANDPVDLFLLPGLAFDKSGRRLGRGGGYYDT 225

Query: 251 FLRNYRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAI 310
           FLR Y++LA E++W+QPLLVALSYS+QI+DDG IP+T ND+ VD LVSPAGVIPI+ AA+
Sbjct: 226 FLRKYQELAEERDWRQPLLVALSYSLQIIDDGAIPVTLNDLPVDVLVSPAGVIPISQAAL 285

Query: 311 QRME 314
            RM+
Sbjct: 286 NRMQ 289
[Close]
BLAST of Spo27282.1 vs. UniProtKB/TrEMBL
Match: D7SIJ5_VITVI (5-formyltetrahydrofolate cyclo-ligase OS=Vitis vinifera GN=VIT_17s0000g05350 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.400e-89
Identity = 168/238 (70.59%), Postives = 199/238 (83.61%), Query Frame = 1 

		  

Query: 74  NSPGDVAATELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRL 133
           N+    + T L+ +FKQK+ +RS VRK+LK MDPS R+ ED+ +Q ++L+APWF+SS+RL
Sbjct: 3   NNTHQSSNTHLEAVFKQKKILRSSVRKALKGMDPSSRSQEDNAVQNIILEAPWFRSSQRL 62

Query: 134 CAYISCRALREVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDL 193
           CAYISC +LREVDTSKVL++ LQ   KD      K LYVPRVEDKNSYMRMLNI+ MDDL
Sbjct: 63  CAYISCSSLREVDTSKVLSEILQNPAKDGHTQMGKKLYVPRVEDKNSYMRMLNITHMDDL 122

Query: 194 IANSMDILEPAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRN 253
           I+NSM+ILEPAPVD  GN+RE+VM AT+ VDLLLLPGLAFDKSGRRLGRGGGYYD FL+N
Sbjct: 123 ISNSMNILEPAPVDGDGNEREDVMQATDPVDLLLLPGLAFDKSGRRLGRGGGYYDAFLKN 182

Query: 254 YRQLANEKNWKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQR 312
           Y +L  E+NWKQPLLVALSYSVQI+D+GVIP+TPNDV VDALVSPAGVIPI  AA +R
Sbjct: 183 YLELVKERNWKQPLLVALSYSVQIMDEGVIPVTPNDVPVDALVSPAGVIPITTAAQER 240
[Close]
BLAST of Spo27282.1 vs. ExPASy Swiss-Prot
Match: 5FCL_ARATH (5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana GN=5FCL PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.100e-82
Identity = 152/230 (66.09%), Postives = 185/230 (80.43%), Query Frame = 1 

		  

Query: 83  ELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRAL 142
           ELD IFKQKR +RS VRKSLK MDPS R  +D+ IQ+ VL+APWFKS + LCAYISC++L
Sbjct: 52  ELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSL 111

Query: 143 REVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSMDILE 202
            EVDTSK+L++ LQ  D + Q    K LYVP VEDKNS MRML+IS M+DL+ANSM+ILE
Sbjct: 112 NEVDTSKILSEILQHPDSNTQ----KKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILE 171

Query: 203 PAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKN 262
           PAPVD+ GN RE+V+ A E +DL +LPGLAFD+ GRRLGRGGGYYDTFL+ Y+  A EK 
Sbjct: 172 PAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKG 231

Query: 263 WKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRM 313
           W+ PL+VALSYS QIL+DG IP+TPNDV +DALV+P+GV+PI P A + M
Sbjct: 232 WRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVPITPRATESM 277
[Close]
BLAST of Spo27282.1 vs. ExPASy Swiss-Prot
Match: MTHFS_HUMAN (5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens GN=MTHFS PE=1 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 1.000e-16
Identity = 60/209 (28.71%), Postives = 111/209 (53.11%), Query Frame = 1 

		  

Query: 91  KRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRALREVDTSKV 150
           KR++R ++++ L+ M   +R  +   + + V+    ++ S+R+  ++S +   E++T ++
Sbjct: 10  KRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQD--EIETEEI 69

Query: 151 LADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDD---LIANSMDILEPAPVD 210
           + D  Q           K  ++PR   ++++M M+ I S ++   L   S +I +P    
Sbjct: 70  IKDIFQRG---------KICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQPGE-- 129

Query: 211 SGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKNWKQPL 270
             G+ RE  + +T  +DL+ +PGL FDK G RLGRG GYYD +L+   Q    K    P 
Sbjct: 130 --GDVREEAL-STGGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEVK----PY 189

Query: 271 LVALSYSVQILDDGVIPITPNDVFVDALV 297
            +AL++  QI     +P+  ND+ VD ++
Sbjct: 190 TLALAFKEQICLQ--VPVNENDMKVDEVL 196
[Close]
BLAST of Spo27282.1 vs. ExPASy Swiss-Prot
Match: MTHFS_RABIT (5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus GN=MTHFS PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.300e-16
Identity = 59/209 (28.23%), Postives = 115/209 (55.02%), Query Frame = 1 

		  

Query: 91  KRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRALREVDTSKV 150
           KR++R+++++ L+ +   +R      + + V+    ++ S+R+  ++S     E++T ++
Sbjct: 10  KRSLRAELKQRLRAISAEERLRCQRLLTQKVIAHRQYQKSQRISIFLSMPD--EIETEEI 69

Query: 151 LADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIA---NSMDILEPAPVD 210
           + D  Q         + K  ++PR   ++++M M+ ++S D++ +    S +I +P+  D
Sbjct: 70  IKDIFQ---------QGKVCFIPRYRLQSNHMDMVKLASADEISSLPKTSWNIHQPSESD 129

Query: 211 SGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKNWKQPL 270
           +    RE  + AT  +DL+ +PGL FD++G RLGRG GYYDT+L+   Q    K    P 
Sbjct: 130 T----REEAL-ATGGLDLIFMPGLGFDRNGNRLGRGRGYYDTYLQRCLQQQGAK----PY 189

Query: 271 LVALSYSVQILDDGVIPITPNDVFVDALV 297
            +AL++  QI     +P+   DV VD ++
Sbjct: 190 TIALAFREQICPQ--VPVDDTDVSVDEVL 196
[Close]
BLAST of Spo27282.1 vs. ExPASy Swiss-Prot
Match: MTHFS_MOUSE (5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus GN=Mthfs PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 5.100e-16
Identity = 62/215 (28.84%), Postives = 118/215 (54.88%), Query Frame = 1 

		  

Query: 91  KRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRALREVDTSKV 150
           KR +R+++++ L+ +   +R  +   + + V+    +++S+R+  ++S +   EV+T  +
Sbjct: 10  KRGLRAELKQRLRALSAEERLRQSLLLTQKVIAHNQYQNSKRISIFLSMQD--EVETEVI 69

Query: 151 LADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIA---NSMDILEPAPVD 210
           + D  +         + K  ++PR + ++++M M+ ++S +++      S +I +P    
Sbjct: 70  IKDIFK---------QGKICFIPRYQFQSNHMDMVRLTSSEEIALLPKTSWNIHQPGE-- 129

Query: 211 SGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKNWKQPL 270
             G+ RE  + +T  +DL+ LPGL FDK G RLGRG GYYDT+L+   Q    K    P 
Sbjct: 130 --GDVREEAL-STGGLDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRCVQHQEVK----PY 189

Query: 271 LVALSYSVQILDDGVIPITPNDVFVDALV---SPA 300
            +AL++  QI     IP+  +D+ VD ++   SPA
Sbjct: 190 TMALAFKEQICPQ--IPVDEHDMKVDEVLYEDSPA 202
[Close]
BLAST of Spo27282.1 vs. ExPASy Swiss-Prot
Match: MTHFS_CAEEL (Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans GN=Y106G6E.4 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.000e-12
Identity = 52/209 (24.88%), Postives = 99/209 (47.37%), Query Frame = 1 

		  

Query: 91  KRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRALREVDTSKV 150
           K  +R  ++  L ++   +   + + +   ++++ WF+ S+RL  Y+S     E+ T  +
Sbjct: 8   KSELRQFMKTLLGKISKEETQRQTEAVFEKIIESKWFQESKRLSVYVSTSG--EIQTDSI 67

Query: 151 LADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNI---SSMDDLIANSMDILEPAPVD 210
           +   L+           K +++P+    ++ M M+ +   ++ D+L +    I +P P  
Sbjct: 68  IQKALEMG---------KEVFIPQFTKGSTAMDMVRVPDQTAFDNLPSTLWGIRQPEP-- 127

Query: 211 SGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKNWKQPL 270
                +    H T  +DL+L PG+AF   G R G G GYYD F   + +   E +   P 
Sbjct: 128 ---KWKWQSYHETGPLDLILAPGVAFSPYGLRCGHGKGYYDRFFSTHHKHFPENS---PK 187

Query: 271 LVALSYSVQILDDGVIPITPNDVFVDALV 297
            + L+   QI+  G IPI+  DV +D ++
Sbjct: 188 KIGLALREQII--GTIPISETDVELDEVI 195
[Close]
BLAST of Spo27282.1 vs. TAIR (Arabidopsis)
Match: AT5G13050.1 (5-formyltetrahydrofolate cycloligase)

HSP 1 Score: 308.1 bits (788), Expect = 6.100e-84
Identity = 152/230 (66.09%), Postives = 185/230 (80.43%), Query Frame = 1 

		  

Query: 83  ELDHIFKQKRTIRSKVRKSLKEMDPSQRAIEDDTIQRLVLDAPWFKSSRRLCAYISCRAL 142
           ELD IFKQKR +RS VRKSLK MDPS R  +D+ IQ+ VL+APWFKS + LCAYISC++L
Sbjct: 52  ELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSL 111

Query: 143 REVDTSKVLADTLQTSDKDKQDFEEKTLYVPRVEDKNSYMRMLNISSMDDLIANSMDILE 202
            EVDTSK+L++ LQ  D + Q    K LYVP VEDKNS MRML+IS M+DL+ANSM+ILE
Sbjct: 112 NEVDTSKILSEILQHPDSNTQ----KKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILE 171

Query: 203 PAPVDSGGNQRENVMHATEAVDLLLLPGLAFDKSGRRLGRGGGYYDTFLRNYRQLANEKN 262
           PAPVD+ GN RE+V+ A E +DL +LPGLAFD+ GRRLGRGGGYYDTFL+ Y+  A EK 
Sbjct: 172 PAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKG 231

Query: 263 WKQPLLVALSYSVQILDDGVIPITPNDVFVDALVSPAGVIPINPAAIQRM 313
           W+ PL+VALSYS QIL+DG IP+TPNDV +DALV+P+GV+PI P A + M
Sbjct: 232 WRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVPITPRATESM 277
The following BLAST results are available for this feature:
BLAST of Spo27282.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902204494|gb|KNA14888.1|8.6e-16599.6hypothetical protein SOVF_1032... [more]
gi|731335271|ref|XP_010678643.1|2.2e-13682.6PREDICTED: 5-formyltetrahydrof... [more]
gi|731335273|ref|XP_010678644.1|4.9e-13682.5PREDICTED: 5-formyltetrahydrof... [more]
gi|870858996|gb|KMT10460.1|9.1e-11483.9hypothetical protein BVRB_5g11... [more]
gi|870858995|gb|KMT10459.1|2.6e-11383.8hypothetical protein BVRB_5g11... [more]
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BLAST of Spo27282.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9R5U6_SPIOL6.0e-16599.65-formyltetrahydrofolate cyclo... [more]
A0A0J8C9Q0_BETVU6.3e-11483.95-formyltetrahydrofolate cyclo... [more]
A0A0J8CE54_BETVU1.8e-11383.85-formyltetrahydrofolate cyclo... [more]
B9I331_POPTR8.9e-9272.55-formyltetrahydrofolate cyclo... [more]
D7SIJ5_VITVI2.4e-8970.55-formyltetrahydrofolate cyclo... [more]
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BLAST of Spo27282.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
5FCL_ARATH1.1e-8266.05-formyltetrahydrofolate cyclo... [more]
MTHFS_HUMAN1.0e-1628.75-formyltetrahydrofolate cyclo... [more]
MTHFS_RABIT1.3e-1628.25-formyltetrahydrofolate cyclo... [more]
MTHFS_MOUSE5.1e-1628.85-formyltetrahydrofolate cyclo... [more]
MTHFS_CAEEL2.0e-1224.8Probable 5-formyltetrahydrofol... [more]
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BLAST of Spo27282.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G13050.16.1e-8466.05-formyltetrahydrofolate cyclo... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0026985-formyltetrahydrofolate cyclo-ligasePANTHERPTHR23407:SF15-FORMYLTETRAHYDROFOLATE CYCLO-LIGASEcoord: 82..316
score: 9.7
IPR0026985-formyltetrahydrofolate cyclo-ligasePFAMPF018125-FTHF_cyc-ligcoord: 91..298
score: 3.0
IPR0026985-formyltetrahydrofolate cyclo-ligaseTIGRFAMsTIGR02727TIGR02727coord: 91..297
score: 1.5
IPR0241855-formyltetrahydrofolate cyclo-ligase-like domainGENE3D3.40.50.10420coord: 90..304
score: 2.2
NoneNo IPR availablePANTHERPTHR23407ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASEcoord: 82..316
score: 9.7
NoneNo IPR availableunknownSSF100950NagB/RpiA/CoA transferase-likecoord: 90..306
score: 1.67

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009396 folic acid-containing compound biosynthetic process
biological_process GO:0046653 tetrahydrofolate metabolic process
molecular_function GO:0030272 5-formyltetrahydrofolate cyclo-ligase activity
molecular_function GO:0005524 ATP binding