Spo00257.1 (mRNA)

Overview
NameSpo00257.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionSterol methyltransferase 2
LocationSpoScf_01740 : 121846 .. 122931 (-)
Sequence length1086
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCATGACCCTGATTTGCACTGTTGGCCTCCTCTTCGTCGGCCTCTATTGGTTTGTCTGCATCCATGGCCCGGCGGAGCAAAAGGGTAAGCGCGCCGTAGATCTCTCCGGCGGCTCAATCTCTTCCGAGAAAGTCCAAGACAAATACGATCAATACTGGTCTTTTTTCCGCCGTCCCAAAGAGATTGAGACGGCAGAGAAGGTCCCCGACTTTGTCGACACCTTCTATAACCTCGTGACCGATATCTACGAGTGGGGATGGGGCCAAAGCTTCCATTTTTCTCCTTCAATCCCCGGGAAATCTCACCGAGATGCCACCCGTCTCCACGAGGAGATGGCTGTCGATCTTATCAAGGTTTCCCCGGGTCAGAAAATCCTCGACGTTGGTTGCGGTGTGGGCGGACCCATGCGGGCCATCGCCGCTCACTCCCGCGCCAAGGTAACCGGGATCACAATCAACGAGTACCAAGTGAAGCGCGCTAAGATGCACAACAAGAAAGCCGGATTAGATTCACTTTGTGAAGTTGTTTGCGGCAATTTCCTTGAGATGCCCTTCGAGAGCAACACCTTTGATGGAGCCTACTCCATTGAAGCCACCTGTCATGCACCCAAATTGGATGACGTGTATGCCGAGGTCTTCCGGGTTTTAAAGCCCGGATCTCTGTATATGTCTTACGAATGGGTAACCACCAGCAAATACAACGGAGATAACGAGGAACACCGCGATGTGATCCAAGGAATCGAGCGAGGGGACGCGCTTCCGGGTCTTAGGAGGTACGATGAGATCGCTGCGGTGGCGAAGAAGGTAGGGTTTGAAGTTGTGGAGGAGAGAGACTTGGCAGCGCCACCATCATTGCCGTGGTGGGACCGTCTTAAGATGGGAAGGATTGCTTACTGGAGGAATCACATTGTGGTCACGGTTCTTGCTGCTATTGGGGTTGCACCAAAAGGAACGGTGGATGTTCATGATATGCTGTTTAAAACCGCAGATTATCTTACACGTGGCGGTGAGACTGGGATATTTAGCCCGATGCATATGATCCTCTGCAGGAAGCCTGTTGATGCTAAGTCCGATTCTTGA

mRNA sequence

ATGGATTCCATGACCCTGATTTGCACTGTTGGCCTCCTCTTCGTCGGCCTCTATTGGTTTGTCTGCATCCATGGCCCGGCGGAGCAAAAGGGTAAGCGCGCCGTAGATCTCTCCGGCGGCTCAATCTCTTCCGAGAAAGTCCAAGACAAATACGATCAATACTGGTCTTTTTTCCGCCGTCCCAAAGAGATTGAGACGGCAGAGAAGGTCCCCGACTTTGTCGACACCTTCTATAACCTCGTGACCGATATCTACGAGTGGGGATGGGGCCAAAGCTTCCATTTTTCTCCTTCAATCCCCGGGAAATCTCACCGAGATGCCACCCGTCTCCACGAGGAGATGGCTGTCGATCTTATCAAGGTTTCCCCGGGTCAGAAAATCCTCGACGTTGGTTGCGGTGTGGGCGGACCCATGCGGGCCATCGCCGCTCACTCCCGCGCCAAGGTAACCGGGATCACAATCAACGAGTACCAAGTGAAGCGCGCTAAGATGCACAACAAGAAAGCCGGATTAGATTCACTTTGTGAAGTTGTTTGCGGCAATTTCCTTGAGATGCCCTTCGAGAGCAACACCTTTGATGGAGCCTACTCCATTGAAGCCACCTGTCATGCACCCAAATTGGATGACGTGTATGCCGAGGTCTTCCGGGTTTTAAAGCCCGGATCTCTGTATATGTCTTACGAATGGGTAACCACCAGCAAATACAACGGAGATAACGAGGAACACCGCGATGTGATCCAAGGAATCGAGCGAGGGGACGCGCTTCCGGGTCTTAGGAGGTACGATGAGATCGCTGCGGTGGCGAAGAAGGTAGGGTTTGAAGTTGTGGAGGAGAGAGACTTGGCAGCGCCACCATCATTGCCGTGGTGGGACCGTCTTAAGATGGGAAGGATTGCTTACTGGAGGAATCACATTGTGGTCACGGTTCTTGCTGCTATTGGGGTTGCACCAAAAGGAACGGTGGATGTTCATGATATGCTGTTTAAAACCGCAGATTATCTTACACGTGGCGGTGAGACTGGGATATTTAGCCCGATGCATATGATCCTCTGCAGGAAGCCTGTTGATGCTAAGTCCGATTCTTGA

Coding sequence (CDS)

ATGGATTCCATGACCCTGATTTGCACTGTTGGCCTCCTCTTCGTCGGCCTCTATTGGTTTGTCTGCATCCATGGCCCGGCGGAGCAAAAGGGTAAGCGCGCCGTAGATCTCTCCGGCGGCTCAATCTCTTCCGAGAAAGTCCAAGACAAATACGATCAATACTGGTCTTTTTTCCGCCGTCCCAAAGAGATTGAGACGGCAGAGAAGGTCCCCGACTTTGTCGACACCTTCTATAACCTCGTGACCGATATCTACGAGTGGGGATGGGGCCAAAGCTTCCATTTTTCTCCTTCAATCCCCGGGAAATCTCACCGAGATGCCACCCGTCTCCACGAGGAGATGGCTGTCGATCTTATCAAGGTTTCCCCGGGTCAGAAAATCCTCGACGTTGGTTGCGGTGTGGGCGGACCCATGCGGGCCATCGCCGCTCACTCCCGCGCCAAGGTAACCGGGATCACAATCAACGAGTACCAAGTGAAGCGCGCTAAGATGCACAACAAGAAAGCCGGATTAGATTCACTTTGTGAAGTTGTTTGCGGCAATTTCCTTGAGATGCCCTTCGAGAGCAACACCTTTGATGGAGCCTACTCCATTGAAGCCACCTGTCATGCACCCAAATTGGATGACGTGTATGCCGAGGTCTTCCGGGTTTTAAAGCCCGGATCTCTGTATATGTCTTACGAATGGGTAACCACCAGCAAATACAACGGAGATAACGAGGAACACCGCGATGTGATCCAAGGAATCGAGCGAGGGGACGCGCTTCCGGGTCTTAGGAGGTACGATGAGATCGCTGCGGTGGCGAAGAAGGTAGGGTTTGAAGTTGTGGAGGAGAGAGACTTGGCAGCGCCACCATCATTGCCGTGGTGGGACCGTCTTAAGATGGGAAGGATTGCTTACTGGAGGAATCACATTGTGGTCACGGTTCTTGCTGCTATTGGGGTTGCACCAAAAGGAACGGTGGATGTTCATGATATGCTGTTTAAAACCGCAGATTATCTTACACGTGGCGGTGAGACTGGGATATTTAGCCCGATGCATATGATCCTCTGCAGGAAGCCTGTTGATGCTAAGTCCGATTCTTGA

Protein sequence

MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIKVSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCGNFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNEEHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAYWRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSDS
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo00257Spo00257gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo00257.1Spo00257.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo00257.1.CDS.1Spo00257.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo00257.1.exon.1Spo00257.1.exon.1exon


Homology
[Close]
BLAST of Spo00257.1 vs. NCBI nr
Match: gi|902114975|gb|KNA03868.1| (hypothetical protein SOVF_204880 [Spinacia oleracea])

HSP 1 Score: 756.1 bits (1951), Expect = 2.700e-215
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR
Sbjct: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG
Sbjct: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE
Sbjct: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY
Sbjct: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD
Sbjct: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. NCBI nr
Match: gi|594553154|gb|AHM10393.1| (sterol methyltransferase 2 [Spinacia oleracea])

HSP 1 Score: 754.6 bits (1947), Expect = 8.000e-215
Identity = 360/361 (99.72%), Postives = 360/361 (99.72%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR
Sbjct: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG
Sbjct: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE
Sbjct: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY
Sbjct: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADY TRGGETGIFSPMHMILCRKPVDAKSD
Sbjct: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYFTRGGETGIFSPMHMILCRKPVDAKSD 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. NCBI nr
Match: gi|731376149|ref|XP_010667472.1| (PREDICTED: 24-methylenesterol C-methyltransferase 2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 695.3 bits (1793), Expect = 5.700e-197
Identity = 329/362 (90.88%), Postives = 344/362 (95.03%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+TLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSIS+EK+QDKY QYWSFFRR
Sbjct: 1   MDSLTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISAEKIQDKYHQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PG+KILDVGCGVGGPMRAIAAHSRA VTGITINEYQVKRAKMHNKKAGLDSLCEVVCG
Sbjct: 121 VGPGKKILDVGCGVGGPMRAIAAHSRANVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPFE NTFDGAYSIEATCHAPKL+DVYAE+ RVLKPGSLY+SYEWVTT KY  ++ 
Sbjct: 181 NFLEMPFEDNTFDGAYSIEATCHAPKLEDVYAEIIRVLKPGSLYVSYEWVTTDKYVNESS 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EHR+VIQGIERGDALPGLR Y+EIA  AKKVGFEVV+E DLAAPP+ PWWDRLKMGRIAY
Sbjct: 241 EHREVIQGIERGDALPGLRAYNEIAEAAKKVGFEVVKELDLAAPPAQPWWDRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDA-KS 360
           WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIF+PMHMILCRKP    +S
Sbjct: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFTPMHMILCRKPNSVPRS 360

Query: 361 DS 362
           DS
Sbjct: 361 DS 362
[Close]
BLAST of Spo00257.1 vs. NCBI nr
Match: gi|645228879|ref|XP_008221201.1| (PREDICTED: 24-methylenesterol C-methyltransferase 2 [Prunus mume])

HSP 1 Score: 659.4 bits (1700), Expect = 3.500e-186
Identity = 309/359 (86.07%), Postives = 332/359 (92.48%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+TL CT  LL  GLYWFVCI GPAEQKGKRAVDLSGGSIS+EKVQD YD+YWSFFRR
Sbjct: 1   MDSLTLFCTGALLAGGLYWFVCILGPAEQKGKRAVDLSGGSISAEKVQDNYDKYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPS+PGKSHRDATRLHEEMAVDLI 
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSLPGKSHRDATRLHEEMAVDLID 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PG +ILDVGCGVGGPMRAIAAHSRA V GITINEYQVKRA++HNKKAGLDSLCEVVCG
Sbjct: 121 VKPGDRILDVGCGVGGPMRAIAAHSRANVVGITINEYQVKRARLHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPF  N+FDGAYSIEATCHAPKL++VYAE+FRVLKPGSLY+SYEWVTT KY GD+ 
Sbjct: 181 NFLEMPFPENSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYKGDDA 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH +VIQGIERGDALPGLR Y +IA  A+KVGFEVV+ERDLA PP+ PWW RLKMGRIAY
Sbjct: 241 EHVEVIQGIERGDALPGLRSYVDIAETARKVGFEVVKERDLANPPAEPWWTRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKS 360
           WRNHI+VT+LAAIGVAPKGTVDVH+MLFKTADYLTRGGE+GIF+PMHMILCRKP  AKS
Sbjct: 301 WRNHILVTLLAAIGVAPKGTVDVHEMLFKTADYLTRGGESGIFTPMHMILCRKPETAKS 359
[Close]
BLAST of Spo00257.1 vs. NCBI nr
Match: gi|823227327|ref|XP_012446491.1| (PREDICTED: 24-methylenesterol C-methyltransferase 2 [Gossypium raimondii])

HSP 1 Score: 659.4 bits (1700), Expect = 3.500e-186
Identity = 309/361 (85.60%), Postives = 331/361 (91.69%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS++L CT  LL  GLYWFVC+ GPAEQKGKRAVDLSGGSIS+EKV+D Y QYWSFFRR
Sbjct: 1   MDSLSLFCTGALLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLI 
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLID 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PG +ILDVGCGVGGPMRAIAAHSRAKV GITIN+YQV RA+MHNKKAGLDSLCEVVCG
Sbjct: 121 VKPGDRILDVGCGVGGPMRAIAAHSRAKVVGITINDYQVNRARMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPF+ N+FDGAYSIEATCHAPKL+DVYAEVFRVLKPGSLY+SYEWVTT KY  DN 
Sbjct: 181 NFLEMPFQDNSFDGAYSIEATCHAPKLEDVYAEVFRVLKPGSLYVSYEWVTTEKYRADNP 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH +VIQGIERGDALPGLR Y +IA  AKKVGFE+V+E+DLA PPSLPWW RLKMGRIAY
Sbjct: 241 EHVEVIQGIERGDALPGLRSYSDIAEAAKKVGFEIVKEKDLAKPPSLPWWTRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHI+V VLAAIG+APKGTVDVHDMLFKTADYLTRGG++GIFSPMHMIL RKP +A S 
Sbjct: 301 WRNHILVIVLAAIGIAPKGTVDVHDMLFKTADYLTRGGDSGIFSPMHMILLRKPKEAPSK 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QAZ4_SPIOL (Methyltransferase OS=Spinacia oleracea GN=SOVF_204880 PE=3 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 1.900e-215
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR
Sbjct: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG
Sbjct: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE
Sbjct: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY
Sbjct: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD
Sbjct: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. UniProtKB/TrEMBL
Match: W8SBA5_SPIOL (Methyltransferase OS=Spinacia oleracea GN=SMT2 PE=2 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 5.500e-215
Identity = 360/361 (99.72%), Postives = 360/361 (99.72%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR
Sbjct: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG
Sbjct: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE
Sbjct: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY
Sbjct: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADY TRGGETGIFSPMHMILCRKPVDAKSD
Sbjct: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYFTRGGETGIFSPMHMILCRKPVDAKSD 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. UniProtKB/TrEMBL
Match: A0A0J8DWX1_BETVU (Methyltransferase OS=Beta vulgaris subsp. vulgaris GN=BVRB_009060 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 4.000e-197
Identity = 329/362 (90.88%), Postives = 344/362 (95.03%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+TLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSIS+EK+QDKY QYWSFFRR
Sbjct: 1   MDSLTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISAEKIQDKYHQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PG+KILDVGCGVGGPMRAIAAHSRA VTGITINEYQVKRAKMHNKKAGLDSLCEVVCG
Sbjct: 121 VGPGKKILDVGCGVGGPMRAIAAHSRANVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPFE NTFDGAYSIEATCHAPKL+DVYAE+ RVLKPGSLY+SYEWVTT KY  ++ 
Sbjct: 181 NFLEMPFEDNTFDGAYSIEATCHAPKLEDVYAEIIRVLKPGSLYVSYEWVTTDKYVNESS 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EHR+VIQGIERGDALPGLR Y+EIA  AKKVGFEVV+E DLAAPP+ PWWDRLKMGRIAY
Sbjct: 241 EHREVIQGIERGDALPGLRAYNEIAEAAKKVGFEVVKELDLAAPPAQPWWDRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDA-KS 360
           WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIF+PMHMILCRKP    +S
Sbjct: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFTPMHMILCRKPNSVPRS 360

Query: 361 DS 362
           DS
Sbjct: 361 DS 362
[Close]
BLAST of Spo00257.1 vs. UniProtKB/TrEMBL
Match: A0A0D2S8Y8_GOSRA (Methyltransferase OS=Gossypium raimondii GN=B456_009G268600 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 2.400e-186
Identity = 309/361 (85.60%), Postives = 331/361 (91.69%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS++L CT  LL  GLYWFVC+ GPAEQKGKRAVDLSGGSIS+EKV+D Y QYWSFFRR
Sbjct: 30  MDSLSLFCTGALLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFRR 89

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLI 
Sbjct: 90  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLID 149

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PG +ILDVGCGVGGPMRAIAAHSRAKV GITIN+YQV RA+MHNKKAGLDSLCEVVCG
Sbjct: 150 VKPGDRILDVGCGVGGPMRAIAAHSRAKVVGITINDYQVNRARMHNKKAGLDSLCEVVCG 209

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPF+ N+FDGAYSIEATCHAPKL+DVYAEVFRVLKPGSLY+SYEWVTT KY  DN 
Sbjct: 210 NFLEMPFQDNSFDGAYSIEATCHAPKLEDVYAEVFRVLKPGSLYVSYEWVTTEKYRADNP 269

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH +VIQGIERGDALPGLR Y +IA  AKKVGFE+V+E+DLA PPSLPWW RLKMGRIAY
Sbjct: 270 EHVEVIQGIERGDALPGLRSYSDIAEAAKKVGFEIVKEKDLAKPPSLPWWTRLKMGRIAY 329

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHI+V VLAAIG+APKGTVDVHDMLFKTADYLTRGG++GIFSPMHMIL RKP +A S 
Sbjct: 330 WRNHILVIVLAAIGIAPKGTVDVHDMLFKTADYLTRGGDSGIFSPMHMILLRKPKEAPSK 389

Query: 361 S 362
           S
Sbjct: 390 S 390
[Close]
BLAST of Spo00257.1 vs. UniProtKB/TrEMBL
Match: B0FDL7_GOSHI (Methyltransferase OS=Gossypium hirsutum GN=SMT2-1 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 5.400e-186
Identity = 309/361 (85.60%), Postives = 331/361 (91.69%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS++L CT  LL  GLYWFVC+ GPAEQKGKRAVDLSGGSIS+EKV+D Y QYWSFFRR
Sbjct: 1   MDSLSLFCTGALLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLI 
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLID 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PG +ILDVGCGVGGPMRAIAAHS+AKV GITIN+YQV RA+MHNKKAGLDSLCEVVCG
Sbjct: 121 VKPGDRILDVGCGVGGPMRAIAAHSQAKVVGITINDYQVNRARMHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFLEMPF+ N+FDGAYSIEATCHAPKL+DVYAEVFRVLKPGSLY+SYEWVTT KY  DN 
Sbjct: 181 NFLEMPFQDNSFDGAYSIEATCHAPKLEDVYAEVFRVLKPGSLYVSYEWVTTEKYRADNP 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH +VIQGIERGDALPGLR Y +IA  AKKVGFEVV+E+DLA PPSLPWW RLKMGRIAY
Sbjct: 241 EHVEVIQGIERGDALPGLRSYSDIAEAAKKVGFEVVKEKDLAKPPSLPWWTRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHI+V VLAAIG+APKGTVDVHDMLFKTADYLTRGG++GIFSPMHMIL RKP +A S 
Sbjct: 301 WRNHILVIVLAAIGIAPKGTVDVHDMLFKTADYLTRGGDSGIFSPMHMILLRKPKEAPSK 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. ExPASy Swiss-Prot
Match: SMT3B_ARATH (24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana GN=SMT3 PE=2 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 2.100e-183
Identity = 293/357 (82.07%), Postives = 329/357 (92.16%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+ L CT GL+   +YWF+C+ GPAE+KGKRA DLSGGSIS+EKV+D Y+QYWSFFR+
Sbjct: 1   MDSVALYCTAGLIAGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFRK 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIE+AEKVPDFVDTFYNLVTDIYEWGWGQSFHFSP +PGKS +DATR+HEEMAVDLIK
Sbjct: 61  PKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PGQKILD GCGVGGPMRAIAAHS+A+VTGITINEYQV+RAK+HNKKAGLDSLC VVCG
Sbjct: 121 VKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFL+MPF+ NTFDGAYSIEATCHAPKL++VY+E+FRV+KPGSL++SYEWVTT KY  D+E
Sbjct: 181 NFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDE 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH+DVIQGIERGDALPGLR Y +IA  AKKVGFEVV+E+DLA PPS PWW+RLKMGRIAY
Sbjct: 241 EHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDA 358
           WRNH+VV +L+AIGVAPKGTVDVH MLFKTADYLTRGGETGIFSPMHMILCRKP  A
Sbjct: 301 WRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGETGIFSPMHMILCRKPEKA 357
[Close]
BLAST of Spo00257.1 vs. ExPASy Swiss-Prot
Match: SMT2_ARATH (24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana GN=SMT2 PE=1 SV=2)

HSP 1 Score: 642.5 bits (1656), Expect = 2.800e-183
Identity = 293/361 (81.16%), Postives = 332/361 (91.97%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+TL  T  L+ VG+YWF+C+ GPAE+KGKRAVDLSGGSIS+EKVQD Y QYWSFFRR
Sbjct: 1   MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEEMAVDLI+
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PGQKILDVGCGVGGPMRAIA+HSRA V GITINEYQV RA++HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFL+MPF+ N+FDGAYSIEATCHAPKL++VYAE++RVLKPGS+Y+SYEWVTT K+  +++
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH +VIQGIERGDALPGLR Y +IA  AKKVGFE+V+E+DLA+PP+ PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHIVV +L+A+GVAPKGTVDVH+MLFKTADYLTRGGETGIFSPMHMILCRKP   +  
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPESPEES 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. ExPASy Swiss-Prot
Match: SMT2_ORYSJ (24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica GN=Smt2-1 PE=2 SV=2)

HSP 1 Score: 554.3 bits (1427), Expect = 9.900e-157
Identity = 258/363 (71.07%), Postives = 301/363 (82.92%), Query Frame = 1 

		  

Query: 1   MDSMTLICTV-GLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFR 60
           M++ T+  T  G+    +YWFV + G AE KGKRAVDL  GSI+++KV+DKY QYWSFFR
Sbjct: 1   MEAATMAWTAAGVGMALVYWFVWVMGAAEVKGKRAVDLKMGSITNDKVKDKYTQYWSFFR 60

Query: 61  RPKEIET----AEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMA 120
           RPKE  T    AEKVP FVDTFYNLVTDIYEWGWGQSFHFSPS+PG+SHR+ATR+HEE  
Sbjct: 61  RPKETATTEASAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHREATRVHEERV 120

Query: 121 VDLIKVSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLC 180
            DL++  PG ++LDVGCGVGGPMRAIAAHS + V GITINEYQV RA+ HN+KAGLDS C
Sbjct: 121 ADLLQAKPGHRLLDVGCGVGGPMRAIAAHSGSNVVGITINEYQVNRARAHNRKAGLDSRC 180

Query: 181 EVVCGNFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKY 240
           EVVCGNFL MPF   +FDGAYSIEATCHAP+L DVY EVFRVLKPG LY+SYEWVTTS Y
Sbjct: 181 EVVCGNFLSMPFSDASFDGAYSIEATCHAPRLQDVYGEVFRVLKPGGLYVSYEWVTTSLY 240

Query: 241 NGDNEEHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKM 300
             DN EH + I GIERGDALPGLRR DEIA++AK+VGFEV++E DLA PP+LPWW RLKM
Sbjct: 241 RADNPEHVEAIHGIERGDALPGLRRQDEIASIAKEVGFEVLKELDLALPPALPWWTRLKM 300

Query: 301 GRIAYWRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPV 359
           GRIAYWRN +VV VL  + +APKG  +VH+ML++TA +LTRGGETGIF+PMHM+L RKPV
Sbjct: 301 GRIAYWRNSLVVRVLTMLRIAPKGVCEVHEMLYETAQHLTRGGETGIFTPMHMVLLRKPV 360
[Close]
BLAST of Spo00257.1 vs. ExPASy Swiss-Prot
Match: SQMT1_BOTBR (Squalene methyltransferase 1 OS=Botryococcus braunii GN=TMT-1 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.300e-76
Identity = 145/339 (42.77%), Postives = 200/339 (59.00%), Query Frame = 1 

		  

Query: 26  PAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRRPKE----IETAEKVPDFVDTFYNLV 85
           P ++   + +DL  G IS EKV   YD Y   +         ++   KV    +TFY+ V
Sbjct: 41  PGQKSRPKLLDLKTGGISFEKVAAVYDDYDKSYGEGDHGELHVKDKNKVFQLANTFYDFV 100

Query: 86  TDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIKVSPGQKILDVGCGVGGPMRAI 145
           TD YEW WG SFHFS  +PG SH  +  LHE      +++ PG K LDVGCGVG P R +
Sbjct: 101 TDGYEWAWGSSFHFSQRMPGLSHAASQMLHESRMASFLRLKPGMKCLDVGCGVGNPGRTV 160

Query: 146 AAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCGNFLEMPFESNTFDGAYSIEAT 205
           A+ S A+VTGITINEYQ+KRA+ HNK+ GL    + V GNF  MPF+  TFD A+++++T
Sbjct: 161 ASCSGAEVTGITINEYQIKRAEYHNKRTGLVGYFKPVVGNFCAMPFKDKTFDAAFAMDST 220

Query: 206 CHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNEEHRDVIQGIERGDALPGLRRY 265
           CHAPKL+DVY+EVFRVLKPG L+ +YEWV+T  Y+ +N  H  V+  I  G+ LP +R +
Sbjct: 221 CHAPKLEDVYSEVFRVLKPGGLFATYEWVSTKDYDPNNSRHVKVMNSIIFGNGLPNIRSW 280

Query: 266 DEIAAVAKKVGFEVVEERDLAAPPSL--PWWD----RLKMGRIAYWRNHIVVTVLAAIGV 325
            +     K VGF++V   DLA  P +  PW+      +K G +   +   +V     +G+
Sbjct: 281 KQAEDAGKNVGFKLVTSFDLATAPPVGKPWYYVPELMVKYGLLTIQK--ALVRGACNVGL 340

Query: 326 APKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKP 355
            P     V +M+      L  GG T IF+PMH+++  KP
Sbjct: 341 LPNEGWKVCNMVADMVPNLVEGGATNIFTPMHLLIFEKP 377
[Close]
BLAST of Spo00257.1 vs. ExPASy Swiss-Prot
Match: SQMT2_BOTBR (Squalene methyltransferase 2 OS=Botryococcus braunii GN=TMT-2 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.400e-72
Identity = 141/362 (38.95%), Postives = 210/362 (58.01%), Query Frame = 1 

		  

Query: 4   MTLICTVGLLFVGLYWFVCIHGPAEQKGK-RAVDLSGGSISSEKVQDKYDQYWSFFRRPK 63
           +T+    GL+   +  ++ I     QK K + +DL+ G I  EKV + ++ Y   + +  
Sbjct: 18  LTVKGATGLIAALILGYIIITRLPGQKTKPKLLDLTAGGIPFEKVGEVFNDYDKSYGKGT 77

Query: 64  E----IETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDL 123
                ++   KV    +TFY+ VTD YEW WG SFHFS  +PG SH  +  LHE      
Sbjct: 78  HGELHVQDTNKVFQLANTFYDFVTDGYEWAWGSSFHFSQRMPGLSHAASQMLHESRMASY 137

Query: 124 IKVSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVV 183
           +++ PG   LDVGCGVG P R +AA S A VTGITIN+YQ++RA+ HN++ GL    +  
Sbjct: 138 LRLKPGMTCLDVGCGVGNPGRTVAACSGAVVTGITINKYQIQRAEYHNRRTGLVGFFKPT 197

Query: 184 CGNFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGD 243
            GNF  MPF++ +FD A++++ATCHAPKL+DVY EVFRVLKPG  + +YEWV+T  Y+  
Sbjct: 198 VGNFCNMPFDAKSFDAAFAMDATCHAPKLEDVYGEVFRVLKPGGFFATYEWVSTKNYDPT 257

Query: 244 NEEHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSL--PWWD----R 303
           N  H  V+  I  G+ LP +R + +     + VGF+++   DLA  P +  PW+      
Sbjct: 258 NTRHVKVMNSIIFGNGLPNIRSWKQAEEAGENVGFKLLTSFDLATAPPVGKPWYYVPELM 317

Query: 304 LKMGRIAYWRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCR 355
           +K G +   +   +V    ++G+ P  +  V +M+      L  GG T IF+PMH+++ +
Sbjct: 318 VKYGLLKIQK--ALVRGACSLGLLPDQSWKVCNMVADMVPNLVEGGATDIFTPMHLLIFQ 377
[Close]
BLAST of Spo00257.1 vs. TAIR (Arabidopsis)
Match: AT1G76090.1 (sterol methyltransferase 3)

HSP 1 Score: 642.9 bits (1657), Expect = 1.200e-184
Identity = 293/357 (82.07%), Postives = 329/357 (92.16%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+ L CT GL+   +YWF+C+ GPAE+KGKRA DLSGGSIS+EKV+D Y+QYWSFFR+
Sbjct: 1   MDSVALYCTAGLIAGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFRK 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIE+AEKVPDFVDTFYNLVTDIYEWGWGQSFHFSP +PGKS +DATR+HEEMAVDLIK
Sbjct: 61  PKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIK 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PGQKILD GCGVGGPMRAIAAHS+A+VTGITINEYQV+RAK+HNKKAGLDSLC VVCG
Sbjct: 121 VKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFL+MPF+ NTFDGAYSIEATCHAPKL++VY+E+FRV+KPGSL++SYEWVTT KY  D+E
Sbjct: 181 NFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDE 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH+DVIQGIERGDALPGLR Y +IA  AKKVGFEVV+E+DLA PPS PWW+RLKMGRIAY
Sbjct: 241 EHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDA 358
           WRNH+VV +L+AIGVAPKGTVDVH MLFKTADYLTRGGETGIFSPMHMILCRKP  A
Sbjct: 301 WRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGETGIFSPMHMILCRKPEKA 357
[Close]
BLAST of Spo00257.1 vs. TAIR (Arabidopsis)
Match: AT1G20330.1 (sterol methyltransferase 2)

HSP 1 Score: 642.5 bits (1656), Expect = 1.600e-184
Identity = 293/361 (81.16%), Postives = 332/361 (91.97%), Query Frame = 1 

		  

Query: 1   MDSMTLICTVGLLFVGLYWFVCIHGPAEQKGKRAVDLSGGSISSEKVQDKYDQYWSFFRR 60
           MDS+TL  T  L+ VG+YWF+C+ GPAE+KGKRAVDLSGGSIS+EKVQD Y QYWSFFRR
Sbjct: 1   MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIK 120
           PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEEMAVDLI+
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 121 VSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCG 180
           V PGQKILDVGCGVGGPMRAIA+HSRA V GITINEYQV RA++HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSKYNGDNE 240
           NFL+MPF+ N+FDGAYSIEATCHAPKL++VYAE++RVLKPGS+Y+SYEWVTT K+  +++
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 241 EHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEVVEERDLAAPPSLPWWDRLKMGRIAY 300
           EH +VIQGIERGDALPGLR Y +IA  AKKVGFE+V+E+DLA+PP+ PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 301 WRNHIVVTVLAAIGVAPKGTVDVHDMLFKTADYLTRGGETGIFSPMHMILCRKPVDAKSD 360
           WRNHIVV +L+A+GVAPKGTVDVH+MLFKTADYLTRGGETGIFSPMHMILCRKP   +  
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPESPEES 360

Query: 361 S 362
           S
Sbjct: 361 S 361
[Close]
BLAST of Spo00257.1 vs. TAIR (Arabidopsis)
Match: AT5G13710.1 (sterol methyltransferase 1)

HSP 1 Score: 263.8 bits (673), Expect = 1.500e-70
Identity = 132/329 (40.12%), Postives = 188/329 (57.14%), Query Frame = 1 

		  

Query: 39  GGSISSEKVQ---DKYDQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHF 98
           GG I    V    +KY+QY   F    E E      D V+ +Y+L T  YE+GWG+SFHF
Sbjct: 8   GGKIDKSDVLTAVEKYEQY-HVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHF 67

Query: 99  SPSIPGKSHRDATRLHEEMAVDLIKVSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITIN 158
           +    G+S R++ + HE      + + PGQK+LDVGCG+GGP+R IA  S + VTG+  N
Sbjct: 68  AQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNN 127

Query: 159 EYQVKRAKMHNKKAGLDSLCEVVCGNFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVF 218
           EYQ+ R K  N+ AG+D  C  V  +F++MPF  N+FD  Y+IEATCHAP     Y E++
Sbjct: 128 EYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIY 187

Query: 219 RVLKPGSLYMSYEWVTTSKYNGDNEEHRDVIQGIERGDALPGLRRYDEIAAVAKKVGFEV 278
           RVLKPG  + +YEW  T  ++ DN EH+ +   IE GD LP +R   +     K+ GFEV
Sbjct: 188 RVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQAGFEV 247

Query: 279 VEERDLAAPPSLPWW----------DRLKMGRIAYWRNHIVVTVLAAIGVAPKGTVDVHD 338
           + E+DLA    +PW+             ++  +  +    +V +L  I +AP+G+  V +
Sbjct: 248 IWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQGSQRVSN 307

Query: 339 MLFKTADYLTRGGETGIFSPMHMILCRKP 355
            L + A+ L  GG   IF+PM+  L RKP
Sbjct: 308 FLEQAAEGLVDGGRREIFTPMYFFLARKP 335
[Close]
BLAST of Spo00257.1 vs. TAIR (Arabidopsis)
Match: AT1G64970.1 (gamma-tocopherol methyltransferase)

HSP 1 Score: 76.6 bits (187), Expect = 3.400e-14
Identity = 48/166 (28.92%), Postives = 73/166 (43.98%), Query Frame = 1 

		  

Query: 77  FYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIKVSPG------------ 136
           FYN  + ++E  WG   H     P  S + +   H+E  + +I+ S              
Sbjct: 67  FYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESLRFAGVTDEEEEKK 126

Query: 137 -QKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLCEVVCGNFL 196
            +K++DVGCG+GG  R +A+   A+  GIT++  Q KRA        L         + L
Sbjct: 127 IKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLAHKASFQVADAL 186

Query: 197 EMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEW 230
           + PFE   FD  +S+E+  H P       E+ RV  PG   +   W
Sbjct: 187 DQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTW 232
[Close]
BLAST of Spo00257.1 vs. TAIR (Arabidopsis)
Match: AT1G73600.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 3.000e-10
Identity = 35/119 (29.41%), Postives = 57/119 (47.90%), Query Frame = 1 

		  

Query: 116 VDLIKVSPGQKILDVGCGVGGPMRAIAAHSRAKVTGITINEYQVKRAKMHNKKAGLDSLC 175
           VD++ + PGQK+LDVGCG+GG    +A +    V GI ++   +  A  H    GL    
Sbjct: 288 VDMLDLKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFALEH--AIGLKCSV 347

Query: 176 EVVCGNFLEMPFESNTFDGAYSIEATCHAPKLDDVYAEVFRVLKPGSLYMSYEWVTTSK 235
           E    +  +  +  NTFD  YS +   H      ++   ++ LKPG   +  ++  + K
Sbjct: 348 EFEVADCTKKEYPDNTFDVIYSRDTILHIQDKPALFRRFYKWLKPGGKVLITDYCRSPK 404
The following BLAST results are available for this feature:
BLAST of Spo00257.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902114975|gb|KNA03868.1|2.7e-215100.hypothetical protein SOVF_2048... [more]
gi|594553154|gb|AHM10393.1|8.0e-21599.7sterol methyltransferase 2 [Sp... [more]
gi|731376149|ref|XP_010667472.1|5.7e-19790.8PREDICTED: 24-methylenesterol ... [more]
gi|645228879|ref|XP_008221201.1|3.5e-18686.0PREDICTED: 24-methylenesterol ... [more]
gi|823227327|ref|XP_012446491.1|3.5e-18685.6PREDICTED: 24-methylenesterol ... [more]
back to top
BLAST of Spo00257.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QAZ4_SPIOL1.9e-215100.Methyltransferase OS=Spinacia ... [more]
W8SBA5_SPIOL5.5e-21599.7Methyltransferase OS=Spinacia ... [more]
A0A0J8DWX1_BETVU4.0e-19790.8Methyltransferase OS=Beta vulg... [more]
A0A0D2S8Y8_GOSRA2.4e-18685.6Methyltransferase OS=Gossypium... [more]
B0FDL7_GOSHI5.4e-18685.6Methyltransferase OS=Gossypium... [more]
back to top
BLAST of Spo00257.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
SMT3B_ARATH2.1e-18382.024-methylenesterol C-methyltra... [more]
SMT2_ARATH2.8e-18381.124-methylenesterol C-methyltra... [more]
SMT2_ORYSJ9.9e-15771.024-methylenesterol C-methyltra... [more]
SQMT1_BOTBR2.3e-7642.7Squalene methyltransferase 1 O... [more]
SQMT2_BOTBR4.4e-7238.9Squalene methyltransferase 2 O... [more]
back to top
BLAST of Spo00257.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G76090.11.2e-18482.0sterol methyltransferase 3[more]
AT1G20330.11.6e-18481.1sterol methyltransferase 2[more]
AT5G13710.11.5e-7040.1sterol methyltransferase 1[more]
AT1G64970.13.4e-1428.9gamma-tocopherol methyltransfe... [more]
AT1G73600.23.0e-1029.4S-adenosyl-L-methionine-depend... [more]
back to top
InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 128..224
score: 3.3
IPR013705Sterol methyltransferase C-terminalPFAMPF08498Sterol_MT_Ccoord: 292..354
score: 1.3
NoneNo IPR availablePANTHERPTHR10108METHYLTRANSFERASEcoord: 4..333
score: 1.0E
NoneNo IPR availablePANTHERPTHR10108:SF85824-METHYLENESTEROL C-METHYLTRANSFERASE 2-RELATEDcoord: 4..333
score: 1.0E

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0006694 steroid biosynthetic process
biological_process GO:0006084 acetyl-CoA metabolic process
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0032259 methylation
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030797 24-methylenesterol C-methyltransferase activity
molecular_function GO:0003838 sterol 24-C-methyltransferase activity