Spo09203.1 (mRNA)

Overview
NameSpo09203.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
Description(C-4 methyl sterol oxidase, putative) (1.14.13.72)
Locationchr6 : 14518102 .. 14523137 (+)
Sequence length1680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTACTTAAATTAAAAGTACAGGAGAAAAATATAGGGGCAGGAGAACGCAGGAAGTCCTCAGAGATTCCCTCTCCCACTTGACAGTTGTGTCTCCCCCCTTTCTTTCTCTCTCTTCTTCTTCTTCTTCGCTCATACGGAGTATCACAAATTCGGAAACTCTTCTTTCTCTCTCTTCTTTCCCTCCCCTCGGAAGATCGGAGTTCGTCTCTCATGGCGTCGCTCCTTGAATCTGGATGGCAGGTCATTTCTCTTTTTCTTTTACGAAATTTTTTATGCTCTCAAGCTTATATTTGATGTCATTTTCTGGTGTTTTTCAACAATATCTGATTAATCTCAATGTTCTCGAGTTTTCCGCTTCGTCGAAGTTAGAATTTGTATGGTTTGTTTATGCGATTACATTAGGTCAAGTCCTTTTTAGCATTACTCTTTAATTGGAATGGCAATTAGTTTGACATTTGTATTAATCTGTCGGAAAAAAGAGATTAGCAAATTCAACTTTTTTTGCTCTAGATTTTATTGTGTATTTTGCATACTTATTGTATTACATGCAAGATCATCTCGTTGTTGGAAGAAATGGTGAATTATGAGGATTATTTTGGTATATGAATCATCGAAACTCATTAGAGATACACAAAGTCCTACAATTATTACTATATTTCAATTCAGGAATGCAGGAGAATCTTGTCATAGTGAGATTCGTAATCGAAATCATATCTCAAATTGAAAACTCATCAATAGTGTACATTAATGTAGCTTCGTTGAAAGCTTTGGTTATGTGTAATCTTGTTTATGCAAGGATAAGGTGAATTGAATGAAGGTTATTTGGAAAGAGAAAGAGCTTAGGGATTGAGTATATTTGAAAAGAAGTTAAAGGAAGGTAAAAGCATATTGGAAGTAGGCTGAGAATTGAAGAGTCTTTAAAATACTAAGGATTAAGGAAGGCCATTGTCTAGTTGTTACTCTCTCCCCCACGCGCTGTCTTGGTTAGTTGTTCATAAAGTTTTAATCCTTTTCGATTAAGGTTTATTTCAGTTAGCTTTGTTAACATCATAGTGGCAGTAATTTCTTCCTAACACAAAATTTGATATTCAGATCACAATCTTGAGTGAGTATTAGCCAACTTTTTTGAGCGTAAATTCGAAGGATACGGCAGTGTGCCGTGTGCAGAAGATAGGGGAGGTGCTGGTTTTACCTCTTTTTATTTTTTATTTTTTTTTATCAATTAGTTAATTCCGTGAACACCAAAACTAGAATTATGTTGATGAGTTAGAGGATAGCAAGCCGCTTCATTCCAAATGAACATCAAATAAATCACAAACATCGAGTGCAGAAAAAACTATGCCTCATCAGCATTAGTGCACCCGGAAGGCATCCTTCTAGGAAGGTTTTCACACTAGAATCCCATCTTGTGCTGAACCTTAGCCACAAATAACAGCCTCAGTGAAATACAGCTAGAGGCTCAGGGATAACCAATTATGTCTAGATTTTTTAACACAAGTTTTGGTATAACACTCTTGCATTCCTCTCTATTAATAAAGCATGAGCATAACCTGCAGTTTCAGTAAGCCAGTGCTCGTGATTTTCCTTGGCTTGTGTGTGCTACGGTGCTACCCTCTGCTCTGTTGTTTAGAACTTCATTAAGCTAGACTTTGCCAAATCTGTCCAGAGTGTCCACTTCTTTCATTCCGTGCCTTGCTCCATACTTTAGCAGTAAGCATTGTGCAGGTGCTTATGTAGTACTAATTCAATTTGCTCATTGCTTGGAACATCCTGTTTTTTCTCTGTATCACATGCTGGTTTGTCATATGTTCTCCTGTCTTTGTTCTCCAGTTCCTTATTCATCTTTTTTCTCCCTAATTTAATTTATATTTAACTTGTCCACCTTATATTGCAGTATTTGGTCACTCATTTCAGCGACTTTCAATTGGCTTGCGTTGGAAGTTTCATCCTTCACGAGAGTGTCTTCTTCTTATCTGGACTCCCTTTTATATTGCTTGAGAGGGGAGGATACCTTAGCAAATACAAGATTCAGGTTCCAATCTTTTTTTTCTTATTGTCTATGTAGAATGGGAATGCTTGGCTTTTAAATAGATTTTTATGTCAAAAACTTTTTCCTTTCACTTCCTATTAAATAATTGTAGTCTACTGCTGCCACTAGATCTTCATCCTATGAGTACTTTGTGATGGAAAATTTATCCTGTTCTTAGTGGATTGATGATCATTGGACATTCACGTTGTTAAAAGTAATTGAATATGGAGTATTTAAATTGTTTTCTGCCTTATGAGTCAAAACTTGCTATAACTCTATGTCCTACAGAACAAAAATAGGCTATTGGAAGATATATATGTTACAATTTCCTGCACTAAGTATAAAAGATGTAGCATATGGTTTATTGCTTATTTGCAAATCATGAGCATTCTGGTTGGTCGCTTATCTCCTAGTTGTGGTCAATTGCAGGCGAAAAATAACCCACCTGAGGCTCAACAGAAATGCATCACACGCTTACTGTTGTACCACTTTTGTGTGAATCTGCCGGTGATGCTCTTATCGTATCCTGTCTTTAGAGCTATGGGGATGCGAAGTAGCCTACCCCTGCCATCCTGGTATCTACAGGTTCTTTATGTTGTAGCTTGTTTACTCTAATCTTCATGCTACCTAGTGACTAGTGTCTTGATGCCTTGGATAATCACTGCTGCTTTTTTTTTTTACTTTCTTTTCTTATGTTTTGTGCAACGCTTGTGTCCATGCTATGCAATCTTTAGGTTGACTCTGGCTCTTTATTTTGCAGGAAAGAAGTGGTACCTCAGATAGTATTTTATTTCATTCTTGAAGATTTTGTGTTTTACTGGGGACACAGGATATTGCATACAAAGTGGCTTTACCAGCATGTTCACAGTGTCCACCATGAGTGAGTCAGATTTTGAAGGCTTTTGGGATTCTTTATTATTTCAGATTCATGACTCTCATTTGGAATTTTTTATTTTTTTATTTTGATATACAGGTATGCTACGCCATTTGGACTGACTTCTGAATATGCTCATCCTGCTGAGATACTATTCCTTGGCTTTGCGACAATTGTCGGACCTGCTATTACAGGTCCCCATTTGTTTACTCTCTGGTTGTGGATGGTCCTTAGAGTGCTTGAGACCGTTGAGGCACACTGTGGCTATCACTTTCCTTGGAGCCCATCAAACTTCATTCCGTTATATGGAGGGTCTGCCTATGAATTTACTTCTTACTATCAAGTCAATTCAAATAGTTATTTGTCTGTATCATGTCATGTGATTTTGTTTTTTTCTTGTGCAGTTCCGATTTTCATGACTATCATCACCGCTTGTTGTTTACAAAGTCTGGGAACTACTCATCAACATTTATGTACATGGACTGGTAAGCATTTTTTTAAAGTTTCCTGATAAACGATAGCTATTCATGGAGGAGATTAAAATTTAAAATAATTGCACTCCTTGGAATGAAGTATTAAGTGAGTCTGCTAGATCGTTCGTCTTTGCTTTGGTGATAGGGAGTAAGGATATAATTAGTCGCCATGGGGGGGAAAAAGGCATGTAGATAAAAGCAGATTATGTTTGACTTGACCTACTTCTGAGAAACTGAAGGCCTTGGCCTGCTAATTGCTTATGCATCATCAGTTAAGATGCTTCAGATTATTTCTTAATGGACTGAAAAATATCAGTCGTAAATCCAGATAGTTAGTAGGGGAAGGTTGTTCTCTTTTTATTTTTATTTTTTTGCAAAGACAGGAGAAGGTCTGCTCAATTCCTTTACTTCTTTAGACTTCAAGTCATCGTTTTTGAAGATTGTACAAAACTGGATGCTATTTGCATGGTTCCCTGTTATTTCCCATTTTAGTTCTTTAGTCGGCCTGAATTGCAAGTAATTCGGTTCAGTGTTCATTGCTTTCTTGGTAATGTTGATTCATATAATCCCATCTACACGATATATGAGAACAGAAACATTACAGTAAAAAAACAAAACAAAAATAACTCGGCAGTAAAGCTGCTGAATGAATATGACGTCGCAATTGGTGGACGTTAGGCTCTGGTAGCTTGCCTGGACTTTGAGTTTCTTAGTTGGTGCTACACTGCATATTTGTTACATTTTTGCAAGTAATCACATCAGGCTAACCAAGTCATGGTTGAATTGTTCCCATTCTTGAAATGAGAAAAATTCCTAATTGCATAGCTGCAAATTTTATTTTTTTGTTGGTGATCTTTGCCTTGACTAATTCTTGAGCTGGAATTTTACATAACATTATGACGACTACCCGTGTTGCAGGATTTTTGGCACTGATAATGGTTACAGAACATTGAAGGCACTGAAGACTGCTGAAGCTGATGGCAAGAAAATGTAAGAAATTGAAGGATTCATTTTTGGTGCCATTCTCTCATTTGGTGTGGGGGGAATGGATTATCAGTCGCTTGATCTTAGTTCTCTATCCCATTCCTTTGATCTTTGTTGTGATTAGTGACCGCAGCCCCTATCATGTGTTTAATTACTTTTTGGTTTGAGGTGGTGTTGGCGGTTAGAACGCTTGTCCTTATCTTCCTTCAATGAATGAAGTTTTAGTTACATGTTAAAGTTATGTTTTCCTTTTACTTTGAAGCTGTGTTGATATGTATTACATATATATAGTGCAGCATGTGAGACCTCTTGCAGAAAGCTGTACAACTTGATATTCTGAAATGATATTTTCTTGTAAATCAAATGCCTTTTTTGGTACGATGTCATGAGTTAAGTGCTAGTATATAATGACACTACCTGTATCTTCACATTATGTTCCATGTAAATGTCGATAGTAATACCTTACTAGTTACAGAGTACTACTGTAGTATTCGTTTGTGTGTGTACCAACGCCTCTAAATTCAAGAATCAATTAATTCCCTTTGAACATTGACTAGGCAATTTCTGTTACTGTTATGATTTGACTTTATGCCATAATGTCATCCGAATGCGTAAACTACAAATTTATTCTTCATGGAGAACGCAGGCAGCAAACAGAACACACCTTTCTCTTC

mRNA sequence

AATTACTTAAATTAAAAGTACAGGAGAAAAATATAGGGGCAGGAGAACGCAGGAAGTCCTCAGAGATTCCCTCTCCCACTTGACAGTTGTGTCTCCCCCCTTTCTTTCTCTCTCTTCTTCTTCTTCTTCGCTCATACGGAGTATCACAAATTCGGAAACTCTTCTTTCTCTCTCTTCTTTCCCTCCCCTCGGAAGATCGGAGTTCGTCTCTCATGGCGTCGCTCCTTGAATCTGGATGGCAGTATTTGGTCACTCATTTCAGCGACTTTCAATTGGCTTGCGTTGGAAGTTTCATCCTTCACGAGAGTGTCTTCTTCTTATCTGGACTCCCTTTTATATTGCTTGAGAGGGGAGGATACCTTAGCAAATACAAGATTCAGGCGAAAAATAACCCACCTGAGGCTCAACAGAAATGCATCACACGCTTACTGTTGTACCACTTTTGTGTGAATCTGCCGGTGATGCTCTTATCGTATCCTGTCTTTAGAGCTATGGGGATGCGAAGTAGCCTACCCCTGCCATCCTGGAAAGAAGTGGTACCTCAGATAGTATTTTATTTCATTCTTGAAGATTTTGTGTTTTACTGGGGACACAGGATATTGCATACAAAGTGGCTTTACCAGCATGTTCACAGTGTCCACCATGAGTATGCTACGCCATTTGGACTGACTTCTGAATATGCTCATCCTGCTGAGATACTATTCCTTGGCTTTGCGACAATTGTCGGACCTGCTATTACAGGTCCCCATTTGTTTACTCTCTGGTTGTGGATGGTCCTTAGAGTGCTTGAGACCGTTGAGGCACACTGTGGCTATCACTTTCCTTGGAGCCCATCAAACTTCATTCCGTTATATGGAGGTTCCGATTTTCATGACTATCATCACCGCTTGTTGTTTACAAAGTCTGGGAACTACTCATCAACATTTATGTACATGGACTGGATTTTTGGCACTGATAATGGTTACAGAACATTGAAGGCACTGAAGACTGCTGAAGCTGATGGCAAGAAAATGTAAGAAATTGAAGGATTCATTTTTGGTGCCATTCTCTCATTTGGTGTGGGGGGAATGGATTATCAGTCGCTTGATCTTAGTTCTCTATCCCATTCCTTTGATCTTTGTTGTGATTAGTGACCGCAGCCCCTATCATGTGTTTAATTACTTTTTGGTTTGAGGTGGTGTTGGCGGTTAGAACGCTTGTCCTTATCTTCCTTCAATGAATGAAGTTTTAGTTACATGTTAAAGTTATGTTTTCCTTTTACTTTGAAGCTGTGTTGATATGTATTACATATATATAGTGCAGCATGTGAGACCTCTTGCAGAAAGCTGTACAACTTGATATTCTGAAATGATATTTTCTTGTAAATCAAATGCCTTTTTTGGTACGATGTCATGAGTTAAGTGCTAGTATATAATGACACTACCTGTATCTTCACATTATGTTCCATGTAAATGTCGATAGTAATACCTTACTAGTTACAGAGTACTACTGTAGTATTCGTTTGTGTGTGTACCAACGCCTCTAAATTCAAGAATCAATTAATTCCCTTTGAACATTGACTAGGCAATTTCTGTTACTGTTATGATTTGACTTTATGCCATAATGTCATCCGAATGCGTAAACTACAAATTTATTCTTCATGGAGAACGCAGGCAGCAAACAGAACACACCTTTCTCTTC

Coding sequence (CDS)

ATGGCGTCGCTCCTTGAATCTGGATGGCAGTATTTGGTCACTCATTTCAGCGACTTTCAATTGGCTTGCGTTGGAAGTTTCATCCTTCACGAGAGTGTCTTCTTCTTATCTGGACTCCCTTTTATATTGCTTGAGAGGGGAGGATACCTTAGCAAATACAAGATTCAGGCGAAAAATAACCCACCTGAGGCTCAACAGAAATGCATCACACGCTTACTGTTGTACCACTTTTGTGTGAATCTGCCGGTGATGCTCTTATCGTATCCTGTCTTTAGAGCTATGGGGATGCGAAGTAGCCTACCCCTGCCATCCTGGAAAGAAGTGGTACCTCAGATAGTATTTTATTTCATTCTTGAAGATTTTGTGTTTTACTGGGGACACAGGATATTGCATACAAAGTGGCTTTACCAGCATGTTCACAGTGTCCACCATGAGTATGCTACGCCATTTGGACTGACTTCTGAATATGCTCATCCTGCTGAGATACTATTCCTTGGCTTTGCGACAATTGTCGGACCTGCTATTACAGGTCCCCATTTGTTTACTCTCTGGTTGTGGATGGTCCTTAGAGTGCTTGAGACCGTTGAGGCACACTGTGGCTATCACTTTCCTTGGAGCCCATCAAACTTCATTCCGTTATATGGAGGTTCCGATTTTCATGACTATCATCACCGCTTGTTGTTTACAAAGTCTGGGAACTACTCATCAACATTTATGTACATGGACTGGATTTTTGGCACTGATAATGGTTACAGAACATTGAAGGCACTGAAGACTGCTGAAGCTGATGGCAAGAAAATGTAA

Protein sequence

MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNNPPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILEDFVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLFTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMYMDWIFGTDNGYRTLKALKTAEADGKKM
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo09203Spo09203gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo09203.1Spo09203.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo09203.1.exon.1Spo09203.1.exon.1exon
Spo09203.1.exon.2Spo09203.1.exon.2exon
Spo09203.1.exon.3Spo09203.1.exon.3exon
Spo09203.1.exon.4Spo09203.1.exon.4exon
Spo09203.1.exon.5Spo09203.1.exon.5exon
Spo09203.1.exon.6Spo09203.1.exon.6exon
Spo09203.1.exon.7Spo09203.1.exon.7exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo09203.1.utr5p.1Spo09203.1.utr5p.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo09203.1.CDS.1Spo09203.1.CDS.1CDS
Spo09203.1.CDS.2Spo09203.1.CDS.2CDS
Spo09203.1.CDS.3Spo09203.1.CDS.3CDS
Spo09203.1.CDS.4Spo09203.1.CDS.4CDS
Spo09203.1.CDS.5Spo09203.1.CDS.5CDS
Spo09203.1.CDS.6Spo09203.1.CDS.6CDS
Spo09203.1.CDS.7Spo09203.1.CDS.7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo09203.1.utr3p.1Spo09203.1.utr3p.1three_prime_UTR


Homology
[Close]
BLAST of Spo09203.1 vs. NCBI nr
Match: gi|902229386|gb|KNA21486.1| (hypothetical protein SOVF_042610 [Spinacia oleracea])

HSP 1 Score: 565.1 bits (1455), Expect = 6.600e-158
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN
Sbjct: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
           PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED
Sbjct: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
           FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY
Sbjct: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240

Query: 241 MDWIFGTDNGYRTLKALKTAEADGKKM 268
           MDWIFGTDNGYRTLKALKTAEADGKKM
Sbjct: 241 MDWIFGTDNGYRTLKALKTAEADGKKM 267
[Close]
BLAST of Spo09203.1 vs. NCBI nr
Match: gi|731348812|ref|XP_010685678.1| (PREDICTED: methylsterol monooxygenase 2-2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 541.2 bits (1393), Expect = 1.000e-150
Identity = 255/263 (96.96%), Postives = 259/263 (98.48%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFI LERGGYLSKYKIQAKNN
Sbjct: 1   MASLIESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFIFLERGGYLSKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
           PPEAQ+KCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED
Sbjct: 61  PPEAQEKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
           FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLL+TKSGNYSSTFMY
Sbjct: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLYTKSGNYSSTFMY 240

Query: 241 MDWIFGTDNGYRTLKALKTAEAD 264
           MD IFGTD+GYRTLK LK AEAD
Sbjct: 241 MDRIFGTDHGYRTLKLLKAAEAD 263
[Close]
BLAST of Spo09203.1 vs. NCBI nr
Match: gi|923800233|ref|XP_013687137.1| (PREDICTED: methylsterol monooxygenase 2-2-like [Brassica napus])

HSP 1 Score: 497.3 bits (1279), Expect = 1.700e-137
Identity = 229/258 (88.76%), Postives = 246/258 (95.35%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSGLPFI LER G+L+KYKIQAKNN
Sbjct: 1   MASLVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLTKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ+KCITRLLLYHFCVNLP+M+ SYPVFRAMGMRSSLPLPSWKEV  QI+FYFI+ED
Sbjct: 61  TPAAQEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSLPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLY+ VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LK+LK
Sbjct: 241 MDWIFGTDKGYRRLKSLK 258
[Close]
BLAST of Spo09203.1 vs. NCBI nr
Match: gi|729416072|ref|XP_010557235.1| (PREDICTED: methylsterol monooxygenase 2-2 [Tarenaya hassleriana])

HSP 1 Score: 496.9 bits (1278), Expect = 2.200e-137
Identity = 227/258 (87.98%), Postives = 245/258 (94.96%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSGLPFI LER G LSKYKIQ KNN
Sbjct: 1   MASLIESGWQYLVTHFSDFQLACIGSFVLHESVFFLSGLPFIYLERAGLLSKYKIQTKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ+KCITRLLLYHFCVNLP+M++SYPVF+ MGM+SSLPLPSWK V  QI+FYFI+ED
Sbjct: 61  SPAAQEKCITRLLLYHFCVNLPLMMVSYPVFKFMGMQSSLPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLY+HVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWM+LRVLETVEAHCGYHFPWSPSNF+PLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 VTLWLWMMLRVLETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFIY 240

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LKALK
Sbjct: 241 MDWIFGTDKGYRKLKALK 258
[Close]
BLAST of Spo09203.1 vs. NCBI nr
Match: gi|685370386|ref|XP_009118962.1| (PREDICTED: methylsterol monooxygenase 2-2-like [Brassica rapa])

HSP 1 Score: 495.7 bits (1275), Expect = 4.900e-137
Identity = 228/258 (88.37%), Postives = 245/258 (94.96%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSGLPFI LER G+L+KYKIQAKNN
Sbjct: 1   MASLVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLTKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ+KCITRLLLYHFCVNLP+M+ SYPVFRAMGMRSS PLPSWKEV  QI+FYFI+ED
Sbjct: 61  TPAAQEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLY+ VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LK+LK
Sbjct: 241 MDWIFGTDKGYRRLKSLK 258
[Close]
BLAST of Spo09203.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RPM7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_042610 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 4.600e-158
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN
Sbjct: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
           PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED
Sbjct: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
           FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY
Sbjct: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240

Query: 241 MDWIFGTDNGYRTLKALKTAEADGKKM 268
           MDWIFGTDNGYRTLKALKTAEADGKKM
Sbjct: 241 MDWIFGTDNGYRTLKALKTAEADGKKM 267
[Close]
BLAST of Spo09203.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BYZ4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g173210 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 7.100e-151
Identity = 255/263 (96.96%), Postives = 259/263 (98.48%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFI LERGGYLSKYKIQAKNN
Sbjct: 1   MASLIESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFIFLERGGYLSKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
           PPEAQ+KCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED
Sbjct: 61  PPEAQEKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
           FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLL+TKSGNYSSTFMY
Sbjct: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLYTKSGNYSSTFMY 240

Query: 241 MDWIFGTDNGYRTLKALKTAEAD 264
           MD IFGTD+GYRTLK LK AEAD
Sbjct: 241 MDRIFGTDHGYRTLKLLKAAEAD 263
[Close]
BLAST of Spo09203.1 vs. UniProtKB/TrEMBL
Match: A0A067L4U4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02933 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.000e-136
Identity = 232/267 (86.89%), Postives = 245/267 (91.76%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGW YL+THFSDFQLAC+GSF LHESVFFLSGLPFI LER G+LSKYKIQ KNN
Sbjct: 1   MASLIESGWLYLITHFSDFQLACLGSFFLHESVFFLSGLPFIYLERAGWLSKYKIQTKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ+KCITRLLLYHF VNLPVML SYPVFR MGMRS LPLPSWK V+ QI+FYFILED
Sbjct: 61  SPAAQEKCITRLLLYHFGVNLPVMLFSYPVFRYMGMRSDLPLPSWKVVLTQIIFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLY+HVHSVHHEYATPFGLTSEYAHPAEILFLGFATI+GPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIIGPAITGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWM+LRVLETVEAHCGYHFPWS SNFIPLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 ITLWLWMMLRVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFIY 240

Query: 241 MDWIFGTDNGYRTLKALKT-AEADGKK 267
           MDWIFGTD GYR LKALKT    DG K
Sbjct: 241 MDWIFGTDKGYRKLKALKTDGVEDGSK 267
[Close]
BLAST of Spo09203.1 vs. UniProtKB/TrEMBL
Match: A0A078JMM6_BRANA (BnaAnng26080D protein OS=Brassica napus GN=BnaAnng26080D PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 1.700e-136
Identity = 227/258 (87.98%), Postives = 245/258 (94.96%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSGLPFI LER G+L+KYKIQAKNN
Sbjct: 1   MASLVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERHGFLTKYKIQAKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ KCITRLLLYHFCVNLP+M+ SYPVFRAMGMRSS PLPSWKEV  QI+FYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILH+KWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LK+LK
Sbjct: 241 MDWIFGTDKGYRRLKSLK 258
[Close]
BLAST of Spo09203.1 vs. UniProtKB/TrEMBL
Match: R0GLY8_9BRAS (Uncharacterized protein (Fragment) OS=Capsella rubella GN=CARUB_v10009860mg PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 1.700e-136
Identity = 227/258 (87.98%), Postives = 242/258 (93.80%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MASL+ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSG PFI LER G+LSKYKIQ KNN
Sbjct: 38  MASLIESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGFPFIYLERAGFLSKYKIQTKNN 97

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ KCITRLLLYHFCVNLP+M+ SYPVFRAMGMRSS PLPSWKEV  QI+FYFI+ED
Sbjct: 98  TPAAQGKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 157

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILH+KWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 158 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 217

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGGSDFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 218 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVY 277

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LK LK
Sbjct: 278 MDWIFGTDKGYRRLKTLK 295
[Close]
BLAST of Spo09203.1 vs. ExPASy Swiss-Prot
Match: SMO22_ARATH (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 4.900e-137
Identity = 226/258 (87.60%), Postives = 241/258 (93.41%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MAS +ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSGLPFI LER G+LSKYKIQ KNN
Sbjct: 1   MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ KCITRLLLYHF VNLP+ML SYPVFRAMGMRSS PLPSWKEV  QI+FYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILH+KWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LK LK
Sbjct: 241 MDWIFGTDKGYRRLKTLK 258
[Close]
BLAST of Spo09203.1 vs. ExPASy Swiss-Prot
Match: SMO21_ARATH (Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana GN=SMO2-1 PE=2 SV=2)

HSP 1 Score: 472.6 bits (1215), Expect = 2.800e-132
Identity = 221/270 (81.85%), Postives = 239/270 (88.52%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           M SL+ESGW+YLVTHFSDFQLAC+GSFILHESVFFLSGLP+I LER G+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            PEAQ KCI RLLLYH CVNLP+M+ SYPVFR MGM SS PLPSWK V  QI+FYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGS------------DFHDYHHRLLF 240
            TLWLWM+LRV+ETVEAHCGYHFPWSPSNF+PLYGGS            DFHDYHHRLL+
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLY 240

Query: 241 TKSGNYSSTFMYMDWIFGTDNGYRTLKALK 259
           TKSGNYSSTF+YMDWIFGTD GYR LKALK
Sbjct: 241 TKSGNYSSTFVYMDWIFGTDKGYRKLKALK 270
[Close]
BLAST of Spo09203.1 vs. ExPASy Swiss-Prot
Match: MSMOB_DICDI (Putative methylsterol monooxygenase DDB_G0269788 OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.800e-62
Identity = 118/263 (44.87%), Postives = 167/263 (63.50%), Query Frame = 1 

		  

Query: 5   LESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQ-AKNNPPE 64
           +E  W   V ++ +  L   G+FI HE  +F S +PF L +   +L KYKIQ  K N  +
Sbjct: 9   IEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKKNEWK 68

Query: 65  AQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILEDFVF 124
            Q  CI ++L+ H  V LP+M +  P  +A+G+ +  PLPS   ++  I   F++EDF F
Sbjct: 69  TQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYF 128

Query: 125 YWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLFTL 184
           YW HR LH  + Y+H+H VHH++A PFG+T+EYAHP E + LG  T++GP +    LFTL
Sbjct: 129 YWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTL 188

Query: 185 WLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMYMDW 244
           W+W+  R+ +TVE H GY FPW+P+  IP +GGS FHD+HH    T  GNYSSTF Y+D 
Sbjct: 189 WVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHE---TFVGNYSSTFTYLDK 248

Query: 245 IFGTDNGYRTLKALKTAE-ADGK 266
           IFGT + Y + K ++ ++ A GK
Sbjct: 249 IFGTSDKYYSRKQIRDSKLAAGK 268
[Close]
BLAST of Spo09203.1 vs. ExPASy Swiss-Prot
Match: MSMOA_DICDI (Putative methylsterol monooxygenase DDB_G0270946 OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2)

HSP 1 Score: 225.3 bits (573), Expect = 7.800e-58
Identity = 106/244 (43.44%), Postives = 156/244 (63.93%), Query Frame = 1 

		  

Query: 5   LESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQ-AKNNPPE 64
           +E  W   V ++ +  L   G+FI H+  +F   +PF++ +   +  KYKIQ  K N  +
Sbjct: 9   IEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTKENDWK 68

Query: 65  AQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILEDFVF 124
           +Q  C  +++L    + LP+M +  P  +A+G+ +  PLPS   ++  +V  FI+EDF F
Sbjct: 69  SQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIEDFYF 128

Query: 125 YWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLFTL 184
           YW HR LH    Y+++H VHH+YA+PFG+T+EYAHP E + LG  T++GP +    LFTL
Sbjct: 129 YWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRDLFTL 188

Query: 185 WLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMYMDW 244
           W+W+ +R+ +TVE H GY FPWS +N IP +GG+ FHDYHH +     GNY+STF Y+D 
Sbjct: 189 WVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEVFI---GNYASTFTYLDK 248

Query: 245 IFGT 248
           IFGT
Sbjct: 249 IFGT 249
[Close]
BLAST of Spo09203.1 vs. ExPASy Swiss-Prot
Match: SMO11_ARATH (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.700e-57
Identity = 112/258 (43.41%), Postives = 152/258 (58.91%), Query Frame = 1 

		  

Query: 5   LESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLE----RGGYLSKYKIQAKNN 64
           LE+ W       SD+ L C     L   VF L  LP + +E      G  ++YKIQ K N
Sbjct: 21  LETLWFDYSATKSDYYLYCHNILFLF-LVFSLVPLPLVFVELARSASGLFNRYKIQPKVN 80

Query: 65  PPEAQQ-KCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILE 124
              +   KC   ++     V  P+ L+SYP  + + +RS LPLP+  E++ Q+V YF++E
Sbjct: 81  YSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVYFLIE 140

Query: 125 DFVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPH 184
           D+  YW HR  H+KW Y  +H VHHEY  P G  + YAH AE+L LG  T +GPAI   H
Sbjct: 141 DYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGH 200

Query: 185 LFTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFM 244
           + T WLW+ LR +E +E H GY FPWSP+ +IP YGG+++HDYHH +      N++S F 
Sbjct: 201 MITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFT 260

Query: 245 YMDWIFGTDNGYRTLKAL 258
           Y D+I+GTD GYR  K L
Sbjct: 261 YCDYIYGTDKGYRFQKKL 277
[Close]
BLAST of Spo09203.1 vs. TAIR (Arabidopsis)
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.800e-138
Identity = 226/258 (87.60%), Postives = 241/258 (93.41%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           MAS +ESGWQYLVTHFSDFQLAC+GSF+LHESVFFLSGLPFI LER G+LSKYKIQ KNN
Sbjct: 1   MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            P AQ KCITRLLLYHF VNLP+ML SYPVFRAMGMRSS PLPSWKEV  QI+FYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILH+KWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMY 240
            TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGG+DFHDYHHRLL+TKSGNYSSTF+Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDNGYRTLKALK 259
           MDWIFGTD GYR LK LK
Sbjct: 241 MDWIFGTDKGYRRLKTLK 258
[Close]
BLAST of Spo09203.1 vs. TAIR (Arabidopsis)
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2)

HSP 1 Score: 472.6 bits (1215), Expect = 1.600e-133
Identity = 221/270 (81.85%), Postives = 239/270 (88.52%), Query Frame = 1 

		  

Query: 1   MASLLESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNN 60
           M SL+ESGW+YLVTHFSDFQLAC+GSFILHESVFFLSGLP+I LER G+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  PPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILED 120
            PEAQ KCI RLLLYH CVNLP+M+ SYPVFR MGM SS PLPSWK V  QI+FYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180
           FVFYWGHRILHTKWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 FTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGS------------DFHDYHHRLLF 240
            TLWLWM+LRV+ETVEAHCGYHFPWSPSNF+PLYGGS            DFHDYHHRLL+
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLY 240

Query: 241 TKSGNYSSTFMYMDWIFGTDNGYRTLKALK 259
           TKSGNYSSTF+YMDWIFGTD GYR LKALK
Sbjct: 241 TKSGNYSSTFVYMDWIFGTDKGYRKLKALK 270
[Close]
BLAST of Spo09203.1 vs. TAIR (Arabidopsis)
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1)

HSP 1 Score: 224.2 bits (570), Expect = 9.800e-59
Identity = 112/258 (43.41%), Postives = 152/258 (58.91%), Query Frame = 1 

		  

Query: 5   LESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLE----RGGYLSKYKIQAKNN 64
           LE+ W       SD+ L C     L   VF L  LP + +E      G  ++YKIQ K N
Sbjct: 21  LETLWFDYSATKSDYYLYCHNILFLF-LVFSLVPLPLVFVELARSASGLFNRYKIQPKVN 80

Query: 65  PPEAQQ-KCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILE 124
              +   KC   ++     V  P+ L+SYP  + + +RS LPLP+  E++ Q+V YF++E
Sbjct: 81  YSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVYFLIE 140

Query: 125 DFVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPH 184
           D+  YW HR  H+KW Y  +H VHHEY  P G  + YAH AE+L LG  T +GPAI   H
Sbjct: 141 DYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGH 200

Query: 185 LFTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFM 244
           + T WLW+ LR +E +E H GY FPWSP+ +IP YGG+++HDYHH +      N++S F 
Sbjct: 201 MITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFT 260

Query: 245 YMDWIFGTDNGYRTLKAL 258
           Y D+I+GTD GYR  K L
Sbjct: 261 YCDYIYGTDKGYRFQKKL 277
[Close]
BLAST of Spo09203.1 vs. TAIR (Arabidopsis)
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2)

HSP 1 Score: 219.2 bits (557), Expect = 3.100e-57
Identity = 111/258 (43.02%), Postives = 149/258 (57.75%), Query Frame = 1 

		  

Query: 5   LESGWQYLVTHFSDFQLACVGSFILHESVFFLSGLPFILLERGGYLS----KYKIQAK-N 64
           LE+ W       SD+ L C     L   +F L  LP + +E     S    +YKIQ K  
Sbjct: 21  LETLWFDYSATKSDYYLYCHNILFLF-LIFSLVPLPLVFIESSQSTSDLFNRYKIQPKVK 80

Query: 65  NPPEAQQKCITRLLLYHFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILE 124
           N   +  KC   ++     V  P+ L+SYP  + + +RS LPLPS  E+V Q+V YF++E
Sbjct: 81  NSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGLPLPSCMEIVAQLVVYFLVE 140

Query: 125 DFVFYWGHRILHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPH 184
           D+  YW HR  H KW Y+  H +HHEY  P G  + YAH AE+L LG  T +GPAI   H
Sbjct: 141 DYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWAEVLLLGIPTFLGPAIAPGH 200

Query: 185 LFTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFM 244
           + T WLW+ LR +E +E H GY FPWS + +IP YGG+++HDYHH +      N++S F 
Sbjct: 201 MITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFT 260

Query: 245 YMDWIFGTDNGYRTLKAL 258
           Y D+I+GTD GYR  K L
Sbjct: 261 YCDYIYGTDKGYRFQKKL 277
[Close]
BLAST of Spo09203.1 vs. TAIR (Arabidopsis)
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3)

HSP 1 Score: 218.8 bits (556), Expect = 4.100e-57
Identity = 105/242 (43.39%), Postives = 147/242 (60.74%), Query Frame = 1 

		  

Query: 17  SDFQLACVGSFILHESVFFLSGLPFILLERGGYLSKYKIQAKNNPPEAQQ-KCITRLLLY 76
           S+F + C    +L   VF L+  P +++E  G+  ++KIQ K     +   +C   ++  
Sbjct: 33  SNFHVYCHTILVLF-LVFSLAPFPLVIVEWTGWFDQFKIQKKVKYSLSDMFQCYKEVMKL 92

Query: 77  HFCVNLPVMLLSYPVFRAMGMRSSLPLPSWKEVVPQIVFYFILEDFVFYWGHRILHTKWL 136
              V   + ++SYP  + +G+RS LPLPS  E+V Q+V YF++ED+  YW HR +H KW 
Sbjct: 93  FLLVVGTLQIVSYPSIQMVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWG 152

Query: 137 YQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLFTLWLWMVLRVLETV 196
           Y+ +H +HHEY +P G  S YAH AEIL LG  T +GPAI   H+ T WLW+ LR  E +
Sbjct: 153 YEKIHRIHHEYTSPIGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAI 212

Query: 197 EAHCGYHFPWSPSNFIPLYGGSDFHDYHHRLLFTKSGNYSSTFMYMDWIFGTDNGYRTLK 256
           E H GY FPWS +  IP YGG ++HDYHH +      N++S F Y D+I+GTD GYR  K
Sbjct: 213 ETHSGYDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHK 272

Query: 257 AL 258
            L
Sbjct: 273 KL 273
The following BLAST results are available for this feature:
BLAST of Spo09203.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902229386|gb|KNA21486.1|6.6e-158100.hypothetical protein SOVF_0426... [more]
gi|731348812|ref|XP_010685678.1|1.0e-15096.9PREDICTED: methylsterol monoox... [more]
gi|923800233|ref|XP_013687137.1|1.7e-13788.7PREDICTED: methylsterol monoox... [more]
gi|729416072|ref|XP_010557235.1|2.2e-13787.9PREDICTED: methylsterol monoox... [more]
gi|685370386|ref|XP_009118962.1|4.9e-13788.3PREDICTED: methylsterol monoox... [more]
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BLAST of Spo09203.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RPM7_SPIOL4.6e-158100.Uncharacterized protein OS=Spi... [more]
A0A0J8BYZ4_BETVU7.1e-15196.9Uncharacterized protein OS=Bet... [more]
A0A067L4U4_JATCU1.0e-13686.8Uncharacterized protein OS=Jat... [more]
A0A078JMM6_BRANA1.7e-13687.9BnaAnng26080D protein OS=Brass... [more]
R0GLY8_9BRAS1.7e-13687.9Uncharacterized protein (Fragm... [more]
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BLAST of Spo09203.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
SMO22_ARATH4.9e-13787.6Methylsterol monooxygenase 2-2... [more]
SMO21_ARATH2.8e-13281.8Methylsterol monooxygenase 2-1... [more]
MSMOB_DICDI1.8e-6244.8Putative methylsterol monooxyg... [more]
MSMOA_DICDI7.8e-5843.4Putative methylsterol monooxyg... [more]
SMO11_ARATH1.7e-5743.4Methylsterol monooxygenase 1-1... [more]
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BLAST of Spo09203.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G07420.12.8e-13887.6sterol 4-alpha-methyl-oxidase ... [more]
AT2G29390.11.6e-13381.8sterol 4-alpha-methyl-oxidase ... [more]
AT4G12110.19.8e-5943.4sterol-4alpha-methyl oxidase 1... [more]
AT4G22756.13.1e-5743.0sterol C4-methyl oxidase 1-2[more]
AT4G22753.14.1e-5743.3sterol 4-alpha methyl oxidase ... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 113..247
score: 2.2
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 6..263
score: 1.2E
NoneNo IPR availablePANTHERPTHR11863:SF27METHYLSTEROL MONOOXYGENASE 2-1-RELATEDcoord: 6..263
score: 1.2E

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006913 nucleocytoplasmic transport
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0046520 sphingoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0007264 small GTPase mediated signal transduction
biological_process GO:0006886 intracellular protein transport
biological_process GO:0006333 chromatin assembly or disassembly
biological_process GO:0080065 4-alpha-methyl-delta7-sterol oxidation
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0000254 C-4 methylsterol oxidase activity
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding