Spo10830.1 (mRNA) | SpinachBase

Overview


Name	Spo10830.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Flavonol synthase
Location	SpoScf_00206 : 233841 .. 235610 (+)
Sequence length	1106

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATAGAAAAAGTTCAAAACATAGCGTTGAACTTCAACAGCATACCATCTGAGTTCATAAGGTCAGAGAGTGAACAGCCAGCAGTAACAACTGTGCATGGGACTGTTCTTGAAGTTCCAACCATTGATTTCAGTGAGGTAGACGATGATAAGCTTGTTGATGTAATTTCAAAGGCTAGCCAAGATTGGGGTTTATTCCAAGTTGTAAATCATGGAATACCAACTCAAGTTATTCAAAATCTGCAAAGAGTTGGTAAGGAATTCTTTGAGCTTCCTCAACAAGAGAAAGAGATTTATTCAAAGCCACCACCAGGTGAAGGTTATGTGGGTGGTGAAGGTTATAGCAATAAGCTCGGTAAAAACACTGATACAGTTCGAGCTTGGGTTGATCATTTGTTCCATAACATTTGGCCTCCCTCTGCTATCAACTTCCATTTTTGGCCCAAGAATCCTCCTTCTTACAGGTAGATAATCTACTTTCTTCCATTTCTTTCCATCTGTTTTTCTTTTTATGTAATTTTTAAGTAATTTGTCTACAAATAAATGAATGAAAACAGAGAGGCAAATGAAGAGTACGCGAAATACTTAATAGGAGTAACAGAGAAGCTGATGAAATGTTGGTCAAAGGGATTGGGATTGGAAGAGAATAAGATAAAAGAGGCAGGAGGTGGTGAAGAAATAGTATATCTTGCTAAGATCAATTACTATCCACCATGTCCACGCCCTGAATTGGCACTTGGAGTTGGAGCACATACAGATTTGAGTATGCTTACAATACTTGTTCCTAATCATGTACCTGGTCTTCAAGTTTGTAGGGATGGTCTCTGGTACAATGTCCGCTACATTCCTAATGCACTCATCATCCACATTGGTGACCAACTTGAGGTACTTTTTAAGAACTTCTATTTATAATAATCAACAATTCAACATGTATAATGTAATAAGTAGTTTAGTTACAAGGGGTAACCATAAATGTGTATAATCACATGCAGGTCCTGAGCAATGGAAAATACAAGGCAGTGCTTCACAGAACAACTGTGAATAAAGAGGAAACAAGGATGTCATGGCCCATCTTCATTGAACCACCACCACACCATGAGGTGGGGCCTGTCTCAGACCTCATCGACGAACACAATCCGGCTAACTACAAGACTAAGAAGTTTTCTGACTACAAATATTGTAAGCTTAACAGGCTCCCTCAGTGATGTCAATCTGAGTTTGTATCATTCAGGTTTCCACTTTGTATCATTCCTATGAATTTCTTTCTTTCCGAATAAACTTCTTGCTTTTACTGTAAACTTCAATGGTTCTGCCAGTTGAAAACAATTTTTTGTGTATTACCAAGTATTTTTTTTGTCTGAATGATCAGAAAACAGGGGCCAACATTTCTATCAGAAGTTTGTCTGATATCAAAAACTTGTAGAGCACAACTAACTGTGTGACATATTATAGACATTTGGTTGAAACTTGAAAGTAATACAAAAAAGAATTCTGTCAATTCTATAACGGAATGTGTCGTGTGGCTTGATCTCGTATACAGAAGACTGATCACTGATAATCAGAGTTCTGACATGCTATTGTGCACTTGACTACCTATACACTTGAAGTACACCTCCATGTATTACACTAGTGTTACATTGCATTAAACTTTGTAAAATATATGCATCAGCTGAATGAGATTTGCTTAACAAACAGGAGACTCACCGATTCTTTTGTGTGAATTTCTCGATGAATTGAAAAAGTCTCGAGCTCTAATTGTACCCCAAT

mRNA sequence

ATGGAGATAGAAAAAGTTCAAAACATAGCGTTGAACTTCAACAGCATACCATCTGAGTTCATAAGGTCAGAGAGTGAACAGCCAGCAGTAACAACTGTGCATGGGACTGTTCTTGAAGTTCCAACCATTGATTTCAGTGAGGTAGACGATGATAAGCTTGTTGATGTAATTTCAAAGGCTAGCCAAGATTGGGGTTTATTCCAAGTTGTAAATCATGGAATACCAACTCAAGTTATTCAAAATCTGCAAAGAGTTGGTAAGGAATTCTTTGAGCTTCCTCAACAAGAGAAAGAGATTTATTCAAAGCCACCACCAGGTGAAGGTTATGTGGGTGGTGAAGGTTATAGCAATAAGCTCGGTAAAAACACTGATACAGTTCGAGCTTGGGTTGATCATTTGTTCCATAACATTTGGCCTCCCTCTGCTATCAACTTCCATTTTTGGCCCAAGAATCCTCCTTCTTACAGAGAGGCAAATGAAGAGTACGCGAAATACTTAATAGGAGTAACAGAGAAGCTGATGAAATGTTGGTCAAAGGGATTGGGATTGGAAGAGAATAAGATAAAAGAGGCAGGAGGTGGTGAAGAAATAGTATATCTTGCTAAGATCAATTACTATCCACCATGTCCACGCCCTGAATTGGCACTTGGAGTTGGAGCACATACAGATTTGAGTATGCTTACAATACTTGTTCCTAATCATGTACCTGGTCTTCAAGTTTGTAGGGATGGTCTCTGGTACAATGTCCGCTACATTCCTAATGCACTCATCATCCACATTGGTGACCAACTTGAGGTCCTGAGCAATGGAAAATACAAGGCAGTGCTTCACAGAACAACTGTGAATAAAGAGGAAACAAGGATGTCATGGCCCATCTTCATTGAACCACCACCACACCATGAGGTGGGGCCTGTCTCAGACCTCATCGACGAACACAATCCGGCTAACTACAAGACTAAGAAGTTTTCTGACTACAAATATTGTAAGCTTAACAGGCTCCCTCAGTGATGTCAATCTGAGTTTGTATCATTCAGGAGACTCACCGATTCTTTTGTGTGAATTTCTCGATGAATTGAAAAAGTCTCGAGCTCTAATTGTACCCCAAT

Coding sequence (CDS)

ATGGAGATAGAAAAAGTTCAAAACATAGCGTTGAACTTCAACAGCATACCATCTGAGTTCATAAGGTCAGAGAGTGAACAGCCAGCAGTAACAACTGTGCATGGGACTGTTCTTGAAGTTCCAACCATTGATTTCAGTGAGGTAGACGATGATAAGCTTGTTGATGTAATTTCAAAGGCTAGCCAAGATTGGGGTTTATTCCAAGTTGTAAATCATGGAATACCAACTCAAGTTATTCAAAATCTGCAAAGAGTTGGTAAGGAATTCTTTGAGCTTCCTCAACAAGAGAAAGAGATTTATTCAAAGCCACCACCAGGTGAAGGTTATGTGGGTGGTGAAGGTTATAGCAATAAGCTCGGTAAAAACACTGATACAGTTCGAGCTTGGGTTGATCATTTGTTCCATAACATTTGGCCTCCCTCTGCTATCAACTTCCATTTTTGGCCCAAGAATCCTCCTTCTTACAGAGAGGCAAATGAAGAGTACGCGAAATACTTAATAGGAGTAACAGAGAAGCTGATGAAATGTTGGTCAAAGGGATTGGGATTGGAAGAGAATAAGATAAAAGAGGCAGGAGGTGGTGAAGAAATAGTATATCTTGCTAAGATCAATTACTATCCACCATGTCCACGCCCTGAATTGGCACTTGGAGTTGGAGCACATACAGATTTGAGTATGCTTACAATACTTGTTCCTAATCATGTACCTGGTCTTCAAGTTTGTAGGGATGGTCTCTGGTACAATGTCCGCTACATTCCTAATGCACTCATCATCCACATTGGTGACCAACTTGAGGTCCTGAGCAATGGAAAATACAAGGCAGTGCTTCACAGAACAACTGTGAATAAAGAGGAAACAAGGATGTCATGGCCCATCTTCATTGAACCACCACCACACCATGAGGTGGGGCCTGTCTCAGACCTCATCGACGAACACAATCCGGCTAACTACAAGACTAAGAAGTTTTCTGACTACAAATATTGTAAGCTTAACAGGCTCCCTCAGTGA

Protein sequence

MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo10830	Spo10830	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo10830.1	Spo10830.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10830.1.exon.1	Spo10830.1.exon.1	exon
Spo10830.1.exon.2	Spo10830.1.exon.2	exon
Spo10830.1.exon.3	Spo10830.1.exon.3	exon
Spo10830.1.exon.4	Spo10830.1.exon.4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10830.1.CDS.1	Spo10830.1.CDS.1	CDS
Spo10830.1.CDS.2	Spo10830.1.CDS.2	CDS
Spo10830.1.CDS.3	Spo10830.1.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10830.1.utr3p.1	Spo10830.1.utr3p.1	three_prime_UTR
Spo10830.1.utr3p.2	Spo10830.1.utr3p.2	three_prime_UTR

Homology

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BLAST of Spo10830.1 vs. NCBI nr
Match: gi|902162176|gb|KNA06651.1| (hypothetical protein SOVF_179140 [Spinacia oleracea])

HSP 1 Score: 707.6 bits (1825), Expect = 1.000e-200
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKA 60
           MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKA
Sbjct: 1   MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKA 60

Query: 61  SQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLG 120
           SQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLG
Sbjct: 61  SQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLG 120

Query: 121 KNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKG 180
           KNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKG
Sbjct: 121 KNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKG 180

Query: 181 LGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQV 240
           LGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQV
Sbjct: 181 LGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQV 240

Query: 241 CRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHH 300
           CRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHH
Sbjct: 241 CRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHH 300

Query: 301 EVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           EVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ
Sbjct: 301 EVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 335

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BLAST of Spo10830.1 vs. NCBI nr
Match: gi|731310587|ref|XP_010687119.1| (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 579.3 bits (1492), Expect = 4.300e-162
Identity = 273/348 (78.45%), Postives = 308/348 (88.51%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALNFN---------SIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDD 60
           ME+EKVQNIA  FN         SIP EFIR+E+EQPA+TT+HG+VLEVPTID ++ D++
Sbjct: 1   MEVEKVQNIAFKFNKNGGKTSVDSIPLEFIRAENEQPAMTTMHGSVLEVPTIDINDFDEE 60

Query: 61  KLVDVISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVG 120
           ++V +ISKAS+DWGLFQ+VNHGIPTQVI NLQRVGKEFFELPQ+EKE+Y+KPPPG  Y G
Sbjct: 61  RVVSLISKASEDWGLFQIVNHGIPTQVIHNLQRVGKEFFELPQEEKEVYAKPPPG--YQG 120

Query: 121 GEGY----SNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLI 180
            EGY    SNK  ++  ++R+WVDHLFHNIWPPSAIN+HFWPKNPPSYREANEEYAKYL 
Sbjct: 121 AEGYGTKLSNKRKEDDVSIRSWVDHLFHNIWPPSAINYHFWPKNPPSYREANEEYAKYLR 180

Query: 181 GVTEKLMKCWSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSML 240
           GVTEKLMKCWSKGLGLEENK+KEAGGGEE+VYLAKINYYPPCP PELALGV AHTDLS+L
Sbjct: 181 GVTEKLMKCWSKGLGLEENKMKEAGGGEEMVYLAKINYYPPCPCPELALGVPAHTDLSLL 240

Query: 241 TILVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETR 300
           TILVPN V GLQV RDGLWYNV YIPNALIIHIGDQLEVLSNGKYKAVLHRTTV KE+TR
Sbjct: 241 TILVPNDVAGLQVSRDGLWYNVDYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVTKEKTR 300

Query: 301 MSWPIFIEPPPHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           MSWP+FIEPP  H+VGP+ +LI+E NPANYKTKKF+DYKYCKLN+LPQ
Sbjct: 301 MSWPVFIEPPAQHQVGPIPELINEQNPANYKTKKFADYKYCKLNKLPQ 346

[Close]

BLAST of Spo10830.1 vs. NCBI nr
Match: gi|330688839|gb|AEC33116.1| (flavonol synthase [Fagopyrum tataricum])

HSP 1 Score: 483.0 bits (1242), Expect = 4.100e-133
Identity = 225/338 (66.57%), Postives = 270/338 (79.88%), Query Frame = 1

		  
Query: 1   MEIEKVQNIAL---NFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+E+VQ IA    + ++IP E+IRSE EQPA+TT  G+VLEVP ID +E ++  LV+ I
Sbjct: 1   MEVERVQAIATLSRSVDTIPLEYIRSEKEQPAITTFQGSVLEVPAIDINESNETSLVESI 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
            KAS++WGLFQVVNHGIP +VI +LQRVGKEFFELP +EKE Y+KP   EG    EGY  
Sbjct: 61  KKASEEWGLFQVVNHGIPIEVISHLQRVGKEFFELPIEEKEEYAKP---EGSKDVEGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           KL K  +  + WVDHLFH IWPPSAIN+ FWPKNP SY+EANEEY K L GV EK+ +C 
Sbjct: 121 KLQKEVEGKKGWVDHLFHRIWPPSAINYKFWPKNPTSYKEANEEYCKCLRGVAEKIFRCM 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GLGLE  ++ EA GG+ + YL KINYYPPCPRP+LALGV AHTD+S LTILVPN VPG
Sbjct: 181 SLGLGLEGPELMEAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV RDGLWY+V+YIPNALI+H+GDQLE++SNGKYKAVLHRTTVNKE+ RMSWP+F+EPP
Sbjct: 241 LQVFRDGLWYDVKYIPNALIVHVGDQLEIMSNGKYKAVLHRTTVNKEKVRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
             H +GP   ++D+ NPA YKTKK+ DY YCKLN+LPQ
Sbjct: 301 AEHVLGPHPKIVDDRNPAKYKTKKYGDYMYCKLNKLPQ 335

[Close]

BLAST of Spo10830.1 vs. NCBI nr
Match: gi|597718006|gb|AHN19765.1| (flavonol synthase [Fagopyrum dibotrys])

HSP 1 Score: 479.2 bits (1232), Expect = 6.000e-132
Identity = 221/338 (65.38%), Postives = 269/338 (79.59%), Query Frame = 1

		  
Query: 1   MEIEKVQNIAL---NFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+E+VQ IA    + ++IP E+IRSE EQPA+TT  G+VLEVP ID +E+++  LV+ I
Sbjct: 1   MEVERVQAIATLSRSVDTIPLEYIRSEKEQPAITTFQGSVLEVPAIDINELNESSLVESI 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
            KAS++WGLFQVVNHGIP +VI +LQRVGKEFFELP +EKE Y+KP   +     EGY  
Sbjct: 61  KKASEEWGLFQVVNHGIPIEVISHLQRVGKEFFELPIEEKEEYAKPEDSKDV---EGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           KL K  +  + WVDHLFH IWPPSAIN+ FWPKNP SY+EANEEY K L GV EK+ +C 
Sbjct: 121 KLQKEVEGKKGWVDHLFHRIWPPSAINYKFWPKNPTSYKEANEEYCKCLRGVAEKIFRCM 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GLGLE  ++ +A GG+ + YL KINYYPPCPRP+LALGV AHTD+S LTILVPN VPG
Sbjct: 181 SLGLGLEGPELMDAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV RDG WY+V+YIPNALI+H+GDQLE++SNGKYKAVLHRTTVNKE+ RMSWP+F+EPP
Sbjct: 241 LQVFRDGFWYDVKYIPNALIVHVGDQLEIMSNGKYKAVLHRTTVNKEKVRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
             H +GP   ++D+ NPA YKTKK+ DY YCKLN+LPQ
Sbjct: 301 AEHVLGPHPKIVDDRNPAKYKTKKYGDYMYCKLNKLPQ 335

[Close]

BLAST of Spo10830.1 vs. NCBI nr
Match: gi|641862045|gb|KDO80732.1| (hypothetical protein CISIN_1g044975mg [Citrus sinensis])

HSP 1 Score: 478.0 bits (1229), Expect = 1.300e-131
Identity = 219/332 (65.96%), Postives = 270/332 (81.33%), Query Frame = 1

		  
Query: 5   KVQNIALNFN-SIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKASQD 64
           +VQ++AL  N +IP +FIRSE+EQP +TTVHG VLEVP ID ++ D +K+   I  ASQ 
Sbjct: 6   RVQDVALKHNDTIPQQFIRSENEQPGITTVHGAVLEVPVIDLNDPDQEKVHRSIVDASQQ 65

Query: 65  WGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLGKNT 124
           WG+FQVVNHGIP +VI+ LQ+VGK FFELPQ+EKE Y+KPP  +     +GY +KL K  
Sbjct: 66  WGMFQVVNHGIPGEVIRELQKVGKMFFELPQEEKEKYAKPPDSKDI---QGYGSKLQKEL 125

Query: 125 DTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKGLGL 184
           +  + WVDHLFH IWPPSAI++ FWP+N PSYREANEEY KYL GV +KL+KC S GLGL
Sbjct: 126 EGKKGWVDHLFHKIWPPSAIDYRFWPENIPSYREANEEYRKYLHGVADKLLKCLSLGLGL 185

Query: 185 EENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQVCRD 244
           EEN +KEA GG+ ++YL KINYYPPCP+PELALGV AHTD+S +T+LVPN V GLQ  RD
Sbjct: 186 EENDMKEALGGDNLIYLLKINYYPPCPQPELALGVVAHTDMSSITLLVPNDVQGLQASRD 245

Query: 245 GLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHHEVG 304
           G WY+V+YIPNAL+IHIGDQ+E+LSNGKYK+VLHRTTV K++TRMSWP+F+EPPP  EVG
Sbjct: 246 GHWYDVKYIPNALVIHIGDQMEILSNGKYKSVLHRTTVTKDKTRMSWPVFLEPPPDQEVG 305

Query: 305 PVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           P   L++E NPA +KTKK+SDY YCKLN++PQ
Sbjct: 306 PHPKLVNEENPAKFKTKKYSDYAYCKLNKIPQ 334

[Close]

BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QJC1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_179140 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 7.200e-201
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKA 60
           MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKA
Sbjct: 1   MEIEKVQNIALNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKA 60

Query: 61  SQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLG 120
           SQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLG
Sbjct: 61  SQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLG 120

Query: 121 KNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKG 180
           KNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKG
Sbjct: 121 KNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKG 180

Query: 181 LGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQV 240
           LGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQV
Sbjct: 181 LGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQV 240

Query: 241 CRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHH 300
           CRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHH
Sbjct: 241 CRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHH 300

Query: 301 EVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           EVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ
Sbjct: 301 EVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 335

[Close]

BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FWD9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g000190 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 3.000e-162
Identity = 273/348 (78.45%), Postives = 308/348 (88.51%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALNFN---------SIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDD 60
           ME+EKVQNIA  FN         SIP EFIR+E+EQPA+TT+HG+VLEVPTID ++ D++
Sbjct: 1   MEVEKVQNIAFKFNKNGGKTSVDSIPLEFIRAENEQPAMTTMHGSVLEVPTIDINDFDEE 60

Query: 61  KLVDVISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVG 120
           ++V +ISKAS+DWGLFQ+VNHGIPTQVI NLQRVGKEFFELPQ+EKE+Y+KPPPG  Y G
Sbjct: 61  RVVSLISKASEDWGLFQIVNHGIPTQVIHNLQRVGKEFFELPQEEKEVYAKPPPG--YQG 120

Query: 121 GEGY----SNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLI 180
            EGY    SNK  ++  ++R+WVDHLFHNIWPPSAIN+HFWPKNPPSYREANEEYAKYL 
Sbjct: 121 AEGYGTKLSNKRKEDDVSIRSWVDHLFHNIWPPSAINYHFWPKNPPSYREANEEYAKYLR 180

Query: 181 GVTEKLMKCWSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSML 240
           GVTEKLMKCWSKGLGLEENK+KEAGGGEE+VYLAKINYYPPCP PELALGV AHTDLS+L
Sbjct: 181 GVTEKLMKCWSKGLGLEENKMKEAGGGEEMVYLAKINYYPPCPCPELALGVPAHTDLSLL 240

Query: 241 TILVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETR 300
           TILVPN V GLQV RDGLWYNV YIPNALIIHIGDQLEVLSNGKYKAVLHRTTV KE+TR
Sbjct: 241 TILVPNDVAGLQVSRDGLWYNVDYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVTKEKTR 300

Query: 301 MSWPIFIEPPPHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           MSWP+FIEPP  H+VGP+ +LI+E NPANYKTKKF+DYKYCKLN+LPQ
Sbjct: 301 MSWPVFIEPPAQHQVGPIPELINEQNPANYKTKKFADYKYCKLNKLPQ 346

[Close]

BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
Match: F5BR59_FAGTA (Flavonol synthase OS=Fagopyrum tataricum GN=FLS PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.900e-133
Identity = 225/338 (66.57%), Postives = 270/338 (79.88%), Query Frame = 1

		  
Query: 1   MEIEKVQNIAL---NFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+E+VQ IA    + ++IP E+IRSE EQPA+TT  G+VLEVP ID +E ++  LV+ I
Sbjct: 1   MEVERVQAIATLSRSVDTIPLEYIRSEKEQPAITTFQGSVLEVPAIDINESNETSLVESI 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
            KAS++WGLFQVVNHGIP +VI +LQRVGKEFFELP +EKE Y+KP   EG    EGY  
Sbjct: 61  KKASEEWGLFQVVNHGIPIEVISHLQRVGKEFFELPIEEKEEYAKP---EGSKDVEGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           KL K  +  + WVDHLFH IWPPSAIN+ FWPKNP SY+EANEEY K L GV EK+ +C 
Sbjct: 121 KLQKEVEGKKGWVDHLFHRIWPPSAINYKFWPKNPTSYKEANEEYCKCLRGVAEKIFRCM 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GLGLE  ++ EA GG+ + YL KINYYPPCPRP+LALGV AHTD+S LTILVPN VPG
Sbjct: 181 SLGLGLEGPELMEAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV RDGLWY+V+YIPNALI+H+GDQLE++SNGKYKAVLHRTTVNKE+ RMSWP+F+EPP
Sbjct: 241 LQVFRDGLWYDVKYIPNALIVHVGDQLEIMSNGKYKAVLHRTTVNKEKVRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
             H +GP   ++D+ NPA YKTKK+ DY YCKLN+LPQ
Sbjct: 301 AEHVLGPHPKIVDDRNPAKYKTKKYGDYMYCKLNKLPQ 335

[Close]

BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
Match: X2GFP2_9CARY (Flavonol synthase OS=Fagopyrum dibotrys PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 4.200e-132
Identity = 221/338 (65.38%), Postives = 269/338 (79.59%), Query Frame = 1

		  
Query: 1   MEIEKVQNIAL---NFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+E+VQ IA    + ++IP E+IRSE EQPA+TT  G+VLEVP ID +E+++  LV+ I
Sbjct: 1   MEVERVQAIATLSRSVDTIPLEYIRSEKEQPAITTFQGSVLEVPAIDINELNESSLVESI 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
            KAS++WGLFQVVNHGIP +VI +LQRVGKEFFELP +EKE Y+KP   +     EGY  
Sbjct: 61  KKASEEWGLFQVVNHGIPIEVISHLQRVGKEFFELPIEEKEEYAKPEDSKDV---EGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           KL K  +  + WVDHLFH IWPPSAIN+ FWPKNP SY+EANEEY K L GV EK+ +C 
Sbjct: 121 KLQKEVEGKKGWVDHLFHRIWPPSAINYKFWPKNPTSYKEANEEYCKCLRGVAEKIFRCM 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GLGLE  ++ +A GG+ + YL KINYYPPCPRP+LALGV AHTD+S LTILVPN VPG
Sbjct: 181 SLGLGLEGPELMDAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV RDG WY+V+YIPNALI+H+GDQLE++SNGKYKAVLHRTTVNKE+ RMSWP+F+EPP
Sbjct: 241 LQVFRDGFWYDVKYIPNALIVHVGDQLEIMSNGKYKAVLHRTTVNKEKVRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
             H +GP   ++D+ NPA YKTKK+ DY YCKLN+LPQ
Sbjct: 301 AEHVLGPHPKIVDDRNPAKYKTKKYGDYMYCKLNKLPQ 335

[Close]

BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
Match: A0A067GZ75_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g044975mg PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 9.300e-132
Identity = 219/332 (65.96%), Postives = 270/332 (81.33%), Query Frame = 1

		  
Query: 5   KVQNIALNFN-SIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKASQD 64
           +VQ++AL  N +IP +FIRSE+EQP +TTVHG VLEVP ID ++ D +K+   I  ASQ 
Sbjct: 6   RVQDVALKHNDTIPQQFIRSENEQPGITTVHGAVLEVPVIDLNDPDQEKVHRSIVDASQQ 65

Query: 65  WGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLGKNT 124
           WG+FQVVNHGIP +VI+ LQ+VGK FFELPQ+EKE Y+KPP  +     +GY +KL K  
Sbjct: 66  WGMFQVVNHGIPGEVIRELQKVGKMFFELPQEEKEKYAKPPDSKDI---QGYGSKLQKEL 125

Query: 125 DTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKGLGL 184
           +  + WVDHLFH IWPPSAI++ FWP+N PSYREANEEY KYL GV +KL+KC S GLGL
Sbjct: 126 EGKKGWVDHLFHKIWPPSAIDYRFWPENIPSYREANEEYRKYLHGVADKLLKCLSLGLGL 185

Query: 185 EENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQVCRD 244
           EEN +KEA GG+ ++YL KINYYPPCP+PELALGV AHTD+S +T+LVPN V GLQ  RD
Sbjct: 186 EENDMKEALGGDNLIYLLKINYYPPCPQPELALGVVAHTDMSSITLLVPNDVQGLQASRD 245

Query: 245 GLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHHEVG 304
           G WY+V+YIPNAL+IHIGDQ+E+LSNGKYK+VLHRTTV K++TRMSWP+F+EPPP  EVG
Sbjct: 246 GHWYDVKYIPNALVIHIGDQMEILSNGKYKSVLHRTTVTKDKTRMSWPVFLEPPPDQEVG 305

Query: 305 PVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           P   L++E NPA +KTKK+SDY YCKLN++PQ
Sbjct: 306 PHPKLVNEENPAKFKTKKYSDYAYCKLNKIPQ 334

[Close]

BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
Match: FLS_EUSER (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum GN=FLS PE=2 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.400e-133
Identity = 218/338 (64.50%), Postives = 272/338 (80.47%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALN---FNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+++VQ IA      ++IP+E+IRSE+EQP ++TVHG VLEVP ID S+ D+ K+V ++
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
           S+AS++WG+FQVVNHGIP +VI+ LQ VGK FFELPQ+EKE+ +KP   EG    EGY  
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKP---EGSQSIEGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           +L K  D  + WVDHLFH IWPPSAIN+ FWPKNPP+YREANEEYAK L  V + L K  
Sbjct: 121 RLQKEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYL 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GL LE N  K+  GG+++VYL KINYYPPCPRP+LALGV AHTD+S +T+LVPN VPG
Sbjct: 181 SLGLDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV +DG WY+ +YIPNALI+HIGDQ+E++SNGKYK+V HRTTVNKE+TRMSWP+F+EPP
Sbjct: 241 LQVYKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           P HEVGP+  L++E NPA +KTKK+ DY YCKLN+LPQ
Sbjct: 301 PDHEVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

[Close]

BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
Match: FLS_PETCR (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum GN=FLS PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 8.100e-129
Identity = 215/340 (63.24%), Postives = 271/340 (79.71%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALN---FNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDF--SEVDDDKLVD 60
           ME+E+VQ I+      ++IPSE+IRSESEQPAVTT+ G VL+VP ID   S   ++ LV+
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  VISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGY 120
           +I++AS++WG+FQVVNHGIP   I  LQ+VGKEFFELPQQEKE+ +KP   EGY G EGY
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKP---EGYQGVEGY 120

Query: 121 SNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMK 180
             KL K     + WVDHLFH IWP SA+N++FWP NPP YREANEEYA  L GV +KL +
Sbjct: 121 GTKLQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFE 180

Query: 181 CWSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHV 240
             S G+GLE++++K+A GG++++Y+ KINYYPPCPRP+LALGV AHTD+S +TILVPN V
Sbjct: 181 ALSLGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEV 240

Query: 241 PGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIE 300
            GLQV +D  WY+V+YIPNALIIHIGDQ+E++SNGKYK+V HRTTVNK++TRMSWP+F+E
Sbjct: 241 QGLQVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLE 300

Query: 301 PPPHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           PPP    GP+S LI + NPA +KTKK+ DY YCKLN+LPQ
Sbjct: 301 PPPELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

[Close]

BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
Match: FLS_PETHY (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida GN=FL PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 8.900e-128
Identity = 211/339 (62.24%), Postives = 269/339 (79.35%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALN---FNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+ +VQ IA      ++IPSE+IRSE+EQPA TT+HG VL+VP ID  + D++K+V +I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFE-LPQQEKEIYSKPPPGEGYVGGEGYS 120
           + AS++WG+FQ++NHGIP + I +LQ+VGKEFFE +PQ+EKE+ +K P   G    EGY 
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTP---GSNDIEGYG 132

Query: 121 NKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKC 180
             L K  +  + WVDHLFH IWPPSA+N+ +WPKNPPSYREANEEY K +  V +++ K 
Sbjct: 133 TSLQKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKS 192

Query: 181 WSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVP 240
            S GLGLE +++ EA GG+EIVYL KINYYPPCPRP+LALGV AHTD+S +TILVPN V 
Sbjct: 193 LSLGLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQ 252

Query: 241 GLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEP 300
           GLQV +DG WY+V+YIPNALI+HIGDQ+E+LSNGKYK+V HRTTVNK++TRMSWP+F+EP
Sbjct: 253 GLQVFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEP 312

Query: 301 PPHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           P  HEVGP+  L+ E NP  +KTKK+ DY YCKLN+LPQ
Sbjct: 313 PSEHEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

[Close]

BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
Match: FLS_CITUN (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu GN=FLS PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 7.600e-127
Identity = 211/338 (62.43%), Postives = 259/338 (76.63%), Query Frame = 1

		  
Query: 1   MEIEKVQNIALNFNS---IPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+E+VQ IA   +S   IP+EFIR E EQPA TT HG   E+PTID  +   D+LV  I
Sbjct: 1   MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSI 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
           ++AS++WG+FQV NHGIP+ +I  LQ VGKEFFELPQ+EKE+YS+P   +     +GY  
Sbjct: 61  AEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDV---QGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           KL K  +  ++WVDHLFH +WPPS+IN+ FWPKNPPSYR  NEEYAKY+  V +KL    
Sbjct: 121 KLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYL 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GLG+E   +KEA GG++I Y+ KINYYPPCPRP+LALGV AHTDLS LT+LVPN VPG
Sbjct: 181 SLGLGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV +D  W + +YIPNAL+IHIGDQ+E+LSNGKYKAVLHRTTVNK++TRMSWP+F+EPP
Sbjct: 241 LQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
               VGP+  L+D+ NP  YK KKF DY YCKLN+LPQ
Sbjct: 301 ADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335

[Close]

BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
Match: FLS_MALDO (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica GN=FLS PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.300e-126
Identity = 207/328 (63.11%), Postives = 258/328 (78.66%), Query Frame = 1

		  
Query: 12  NFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKASQDWGLFQVVN 71
           N  +IP+EFIRSE+EQP +TTVHG VLEVP IDFS+ D++KL+  I++AS +WG++Q+VN
Sbjct: 13  NEGTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVN 72

Query: 72  HGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLGKNTD----TVR 131
           H IP++VI  LQ VGKEFFELPQ+EKE Y+KPP        EGY  KL K       T +
Sbjct: 73  HDIPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASI---EGYGTKLFKEISEGDTTKK 132

Query: 132 AWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKGLGLEENK 191
            WVD+LF+ IWPPS +N+ FWPKNPPSYREANEEYAK+L  V EKL +  S GLGLE  +
Sbjct: 133 GWVDNLFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQE 192

Query: 192 IKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQVCRDGLWY 251
           +K+A GG+ + YL KINYYPPCPRP+LALGV AHTD+S +TILVPN V GLQ C+DG WY
Sbjct: 193 LKKAAGGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWY 252

Query: 252 NVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHHEVGPVSD 311
           +V+YIPNAL+IHIGDQ+E++SNGKY +VLHRTTVNK++TR+SWP+F+EPP  H VGP   
Sbjct: 253 DVKYIPNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQ 312

Query: 312 LIDEHNPANYKTKKFSDYKYCKLNRLPQ 336
           L++  N   YKTKK+ DY YCK+N+LPQ
Sbjct: 313 LVNAVNQPKYKTKKYGDYVYCKINKLPQ 337

[Close]

BLAST of Spo10830.1 vs. TAIR (Arabidopsis)
Match: AT5G08640.1 (flavonol synthase 1)

HSP 1 Score: 407.9 bits (1047), Expect = 6.000e-114
Identity = 191/337 (56.68%), Postives = 247/337 (73.29%), Query Frame = 1

		  
Query: 1   MEIEKVQNIA---LNFNSIPSEFIRSESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVI 60
           ME+E+VQ+I+   L   +IP EFIRSE EQPA+TT  G    +P +D S+ D++ +   +
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  SKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSN 120
            KAS++WGLFQVVNHGIPT++I+ LQ VG++FFELP  EKE  +KP   +     EGY  
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDI---EGYGT 120

Query: 121 KLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCW 180
           KL K+ +  +AWVDHLFH IWPPS +N+ FWPKNPP YRE NEEYA ++  ++E L+   
Sbjct: 121 KLQKDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGIL 180

Query: 181 SKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTILVPNHVPG 240
           S GLGL+ + +KE  GGE   Y+ KINYYPPCPRP+LALGV AHTDLS +T+LVPN VPG
Sbjct: 181 SDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPG 240

Query: 241 LQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMSWPIFIEPP 300
           LQV +D  W++  YIP+A+I+HIGDQ+  LSNG+YK VLHRTTV+KE+TRMSWP+F+EPP
Sbjct: 241 LQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPP 300

Query: 301 PHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKLNRLP 335
               VGP+ +L  + NP  +K   F DY Y KLN+LP
Sbjct: 301 REKIVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Spo10830.1 vs. TAIR (Arabidopsis)
Match: AT5G63590.1 (flavonol synthase 3)

HSP 1 Score: 315.8 bits (808), Expect = 3.100e-86
Identity = 149/301 (49.50%), Postives = 200/301 (66.45%), Query Frame = 1

		  
Query: 34  HGTVLEVPTIDFSEVDDDKLVDVISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELP 93
           H + L++P ID S  D++ +   + KASQ+WG+FQVVNHGIPT++I  L +VG EFFELP
Sbjct: 8   HISSLDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELP 67

Query: 94  QQEKEIYSKPPPGEGYVGGEGYSNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPP 153
           + EKE  +KP   E  +  EGY  K  K+ +   AWVDHLFH IWPPS +N  FWPKNPP
Sbjct: 68  ETEKEAVAKP---EDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPP 127

Query: 154 SYREANEEYAKYLIGVTEKLMKCWSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPE 213
            Y E NEEYA ++  ++EK+M+  S+GLGL    +KE  GGE I YL KINYYPPCP PE
Sbjct: 128 EYIEVNEEYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPE 187

Query: 214 LALGVGAHTDLSMLTILVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYK 273
           L +G   HTD++ +T+LV N   GLQ  +D  W +  Y  + +I+ IGDQ   +SNGKYK
Sbjct: 188 LVVGAPDHTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYK 247

Query: 274 AVLHRTTVNKEETRMSWPIFIEPPPHHEVGPVSDLI-DEHNPANYKTKKFSDYKYCKLNR 333
           +V HR  ++KE+TR+SWP+F+E       GP+ +LI  + N   +K   + DYK+ KL +
Sbjct: 248 SVEHRAKMDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKK 305

[Close]

BLAST of Spo10830.1 vs. TAIR (Arabidopsis)
Match: AT5G63600.2 (flavonol synthase 5)

HSP 1 Score: 283.5 bits (724), Expect = 1.700e-76
Identity = 132/304 (43.42%), Postives = 198/304 (65.13%), Query Frame = 1

		  
Query: 23  SESEQPAVTTVHGTVLEVPTIDFSEVDDDKLVDVISKASQDWGLFQVVNHGIPTQVIQNL 82
           S S+Q   +T+ G+ ++VP +D S  D+D LV  + KAS++WG+FQVVNHGIPT++++ L
Sbjct: 16  SLSKQLESSTLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQL 75

Query: 83  QRVGKEFFELPQQEKEIYSKPPPGEGYVGGEGYSNKLGKNTDTVRAWVDHLFHNIWPPSA 142
           Q VG +FFELP  EKE  +K    EGY       N LG     +  W +HLFH + PPS 
Sbjct: 76  QMVGTQFFELPDAEKETVAKEEDFEGYK-----KNYLGG----INNWDEHLFHRLSPPSI 135

Query: 143 INFHFWPKNPPSYREANEEYAKYLIGVTEKLMKCWSKGLGLEENKIKEAGGGEEIVYLAK 202
           IN+ +WPKNPP YRE  EEY K++  +TEK++   S+GLGL+     ++ GG+   Y+ +
Sbjct: 136 INYKYWPKNPPQYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLR 195

Query: 203 INYYPPCPRPELALGVGAHTDLSMLTILVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGD 262
           +N+YPP    EL +G  AH+D+  + +L+PN VPGLQ  +D  W ++ YI +A+++ IGD
Sbjct: 196 VNFYPPTQDTELVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGD 255

Query: 263 QLEV-LSNGKYKAVLHRTTVNKEETRMSWPIFIEPPPHHEVGPVSDLIDEHNPANYKTKK 322
           QL V ++NG+ K VLHR   +K++ R+SWP+F+ P     VGP+ +   + NP  ++T  
Sbjct: 256 QLMVWMTNGRLKNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLI 310

Query: 323 FSDY 326
           ++DY
Sbjct: 316 YNDY 310

[Close]

BLAST of Spo10830.1 vs. TAIR (Arabidopsis)
Match: AT4G22880.1 (leucoanthocyanidin dioxygenase)

HSP 1 Score: 272.7 bits (696), Expect = 3.000e-73
Identity = 141/342 (41.23%), Postives = 211/342 (61.70%), Query Frame = 1

		  
Query: 3   IEKVQNIALN-FNSIPSEFIRSESEQPAVTTVHGTVL-----EVPTIDFSEV--DDDKL- 62
           +E+V+++A +   SIP E+IR + E  ++  V          +VPTID   +  DD+K+ 
Sbjct: 4   VERVESLAKSGIISIPKEYIRPKEELESINDVFLEEKKEDGPQVPTIDLKNIESDDEKIR 63

Query: 63  ---VDVISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKEIYSKPPPGEGYV 122
              ++ + KAS DWG+  ++NHGIP  +++ +++ G+EFF L  +EKE Y+      G +
Sbjct: 64  ENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQ-ATGKI 123

Query: 123 GGEGYSNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSYREANEEYAKYLIGVT 182
             +GY +KL  N      W D+ FH  +P    +   WPK P  Y EA  EYAK L  + 
Sbjct: 124 --QGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLA 183

Query: 183 EKLMKCWSKGLGLEENKI-KEAGGGEEIVYLAKINYYPPCPRPELALGVGAHTDLSMLTI 242
            K+ K  S GLGLE +++ KE GG EE++   KINYYP CP+PELALGV AHTD+S LT 
Sbjct: 184 TKVFKALSVGLGLEPDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTF 243

Query: 243 LVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVNKEETRMS 302
           ++ N VPGLQ+  +G W   + +P+++++HIGD LE+LSNGKYK++LHR  VNKE+ R+S
Sbjct: 244 ILHNMVPGLQLFYEGKWVTAKCVPDSIVMHIGDTLEILSNGKYKSILHRGLVNKEKVRIS 303

Query: 303 WPIFIEPPPHHEV-GPVSDLIDEHNPANYKTKKFSDYKYCKL 331
           W +F EPP    V  P+ +++   +PA +  + F+ +   KL
Sbjct: 304 WAVFCEPPKDKIVLKPLPEMVSVESPAKFPPRTFAQHIEHKL 342

[Close]

BLAST of Spo10830.1 vs. TAIR (Arabidopsis)
Match: AT5G43935.1 (flavonol synthase 6)

HSP 1 Score: 261.5 bits (667), Expect = 6.900e-70
Identity = 126/297 (42.42%), Postives = 187/297 (62.96%), Query Frame = 1

		  
Query: 39  EVPTIDFSEVDDDKLVDVISKASQDWGLFQVVNHGIPTQVIQNLQRVGKEFFELPQQEKE 98
           ++P +D S+  D+ +   + KAS++WG+FQ+VNHGIP ++++ LQ VG++FFELP  EKE
Sbjct: 18  KIPIVDLSDPSDELVAHAVVKASEEWGIFQLVNHGIPAELMRRLQEVGRQFFELPASEKE 77

Query: 99  IYSKPPPGEGYVGGEGYSNKLGKNTDTVRAWVDHLFHNIWPPSAINFHFWPKNPPSY--- 158
             ++P   +     EG+ +K  K    ++AW DHL HNIWPPS+IN+ +WP NP  Y   
Sbjct: 78  SVTRPADSQDI---EGFFSKDPKK---LKAWDDHLIHNIWPPSSINYRYWPNNPSDYSGD 137

Query: 159 --REANEEYAKYLIGVTEKLMKCWSKGLGLEENKIKEAGGGEEIVYLAKINYYPPCPRPE 218
             RE  +EY + +  +TEK++                  GG++  Y+ +INYYPP    +
Sbjct: 138 GFREVTKEYTRNVTNLTEKIV------------------GGDKAQYVMRINYYPPS---D 197

Query: 219 LALGVGAHTDLSMLTILVPNHVPGLQVCRDGLWYNVRYIPNALIIHIGDQLEVLSNGKYK 278
            A+G  AHTD   L +LV N VPGLQV +D  W++V YI +A+I+ IGDQ+  +SNGKYK
Sbjct: 198 SAIGAPAHTDFCGLALLVSNEVPGLQVFKDDHWFDVEYINSAVIVLIGDQIMRMSNGKYK 257

Query: 279 AVLHRTTVNKEETRMSWPIFIEPPPHHEVGPVSDLIDEHNPANYKTKKFSDYKYCKL 331
            VLHR+ ++ ++TRMSWPI +EP     VGP+ +L  + NP  +++  F DY Y K+
Sbjct: 258 NVLHRSIMDAKKTRMSWPILVEPKRGLVVGPLPELTGDENPPKFESLTFEDYVYRKI 287

The following BLAST results are available for this feature:

BLAST of Spo10830.1 vs. NCBI nr
BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
BLAST of Spo10830.1 vs. TAIR (Arabidopsis)

BLAST of Spo10830.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902162176\|gb\|KNA06651.1\|	1.0e-200	100.	hypothetical protein SOVF_1791...	[more]
gi\|731310587\|ref\|XP_010687119.1\|	4.3e-162	78.4	PREDICTED: flavonol synthase/f...	[more]
gi\|330688839\|gb\|AEC33116.1\|	4.1e-133	66.5	flavonol synthase [Fagopyrum t...	[more]
gi\|597718006\|gb\|AHN19765.1\|	6.0e-132	65.3	flavonol synthase [Fagopyrum d...	[more]
gi\|641862045\|gb\|KDO80732.1\|	1.3e-131	65.9	hypothetical protein CISIN_1g0...	[more]

BLAST of Spo10830.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9QJC1_SPIOL	7.2e-201	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8FWD9_BETVU	3.0e-162	78.4	Uncharacterized protein OS=Bet...	[more]
F5BR59_FAGTA	2.9e-133	66.5	Flavonol synthase OS=Fagopyrum...	[more]
X2GFP2_9CARY	4.2e-132	65.3	Flavonol synthase OS=Fagopyrum...	[more]
A0A067GZ75_CITSI	9.3e-132	65.9	Uncharacterized protein OS=Cit...	[more]

BLAST of Spo10830.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
FLS_EUSER	1.4e-133	64.5	Flavonol synthase/flavanone 3-...	[more]
FLS_PETCR	8.1e-129	63.2	Flavonol synthase/flavanone 3-...	[more]
FLS_PETHY	8.9e-128	62.2	Flavonol synthase/flavanone 3-...	[more]
FLS_CITUN	7.6e-127	62.4	Flavonol synthase/flavanone 3-...	[more]
FLS_MALDO	1.3e-126	63.1	Flavonol synthase/flavanone 3-...	[more]

BLAST of Spo10830.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
AT5G08640.1	6.0e-114	56.6	flavonol synthase 1	[more]
AT5G63590.1	3.1e-86	49.5	flavonol synthase 3	[more]
AT5G63600.2	1.7e-76	43.4	flavonol synthase 5	[more]
AT4G22880.1	3.0e-73	41.2	leucoanthocyanidin dioxygenase	[more]
AT5G43935.1	6.9e-70	42.4	flavonol synthase 6	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002283	Isopenicillin N synthase	PRINTS	PR00682	IPNSYNTHASE	coord: 256..282 score: 5.8E-9coord: 57..74 score: 5.8E-9coord: 221..242 score: 5.
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 203..296 score: 2.8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 196..296 score: 13
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 40..149 score: 2.3
IPR027443	Isopenicillin N synthase-like	GENE3D	2.60.120.330		coord: 12..332 score: 5.5
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..335 score: 6.9E
None	No IPR available	PANTHER	PTHR10209:SF165	FLAVONOL SYNTHASE 3-RELATED	coord: 1..335 score: 6.9E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 9..332 score: 6.46

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0051555	flavonol biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0045431	flavonol synthase activity
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0045486	naringenin 3-dioxygenase activity