Spo23280.1 (mRNA) | SpinachBase

Overview


Name	Spo23280.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Cinnamoyl-CoA reductase 1
Location	chr6 : 44079859 .. 44084236 (-)
Sequence length	1582

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAACTTCTTCTATTAAAACCCCATTATTATTTATTATTATTTTCATAACTATATAAATAAATAATACACTCTCTTCACTAACTTACTCTTACAATTTTCCCACCAACATAAACCACCAAAAATGGCCCCTGCATCATTCATCCCCATTTCAAACACAGTATGTGTTATGGATGCGTCCGGTTCACTCGGTTCGAGTCTCGTAAACCGGCTTCTTCAACGTGGTTACATTGTTCATGCCGCGGTTCAACCTCACGGTAATTAATTAGCAATGATTTTGATTAATTAGCTTAATTTTGATTATTAATTGAGGTTAATTTATTAATTCATTATGTTTTTTGTATGTTATTATAGGTGAAGTGAAGGGATTGGAGGGGTTAAGTGATGATGATGGAAAAGAAAGGTTGAAGATATTTGATTGTGATGTGTTTGATTATCATAGTATACTTGATGTTTTGAAGGGTTGCTCTGCTTTGTTCTATAACTTTGAACTTCCTTTTGATCAACCTGTCTATGATGTAAGTTTCATTTTTCCCACTTTTTGGTTTTTGTTAGCTTAATTAAGTACTATGATAAAAAAAGATTAATTTCCTTTAACAAAACTTATGACATAATAATTTCTTGATAAACAACCTAGATTAGTACTAAGTACTAATTTTAGTAAAACTACAAAATTTTCGATCTTTGCTCTTTCTATTATTAGCAAGTTACTTTTTTAATGAATAATTTAGGCCCGGTTCTCGCCTTATTTCAGGAAAAATAAATGCAGATAAGTTCAGGTAATAGAAGTTCATGAAAAAATAAGGATTATCTAAGTACAATTTATAACTAGGATTTTTTGGATAAAACCACCTTTTAAAGTTCAACTTTTTGAAATAACCACCTTATATATTTTTTTTTAAAAAAAACCACCTTAAACTTCCAAAGTTTAAGAAATCACCACCTTCTGTTAACTTCAGTCAAATGTTCCGTCAAATGTTCCGTTAACTTCCAAATATTTGGCCGTTGGATGTGGGGCCCACTGACGGAACATTTGACGAAAGTTAACAGAAGGTGGTGATTTCTTAAACTTTGGAAGTTTAAGGTGGTTTTTTTTTAAAAAAAACATATAAGGTGGTTATTTCAAAAAGTTGAACTTTAAAAGGTGGTTTTATCCAAAAAATCCTTATAACTAAAATAAGTTCTGATAAATTCAGTTAACTTCAGGAAAAAAAAAAAAGGTCAAAATTAGGTGAATAGAACGCACCATTAGATATAATATCCATAGACGAATTTGGCTTGATTGTAGGATTAATATTTATTGAATTGTGTTAAATATTATAATTATCCTTCATTTTAAAATTTACCCTTTGCCAGTCCAATATAGTACACATTTTTCCTTGGAATACATTTGAGAAAGTTAAAAAATTCTACTTATGAAATTTGAAAAATGGGATATTATTAAAAATTATTTGAACAATTGGAAAGAAAAAAAAAAGTCTAATAGACTACTCTGTATTTTTATTAAAAATAAGAAAAAATGAAGTATTCTATCATAAACAAAAAGAAACAGAGAAAGTAACTGTAACCGTTTTCGTCCATGTATTAAGCCACTTTTGGAAAAGAGTTGCAACTTTTGGAAGTAGATTAGGATGGTTACCTATCAATCTCCTTGAACTTAAATACAGCAAAATTGAAGCTGGATTTGAAATGCTTTAAATAAACAACAGCCGATTATTAGGTTGGAGAGATCGAACAATTAATTTGATTCAACCATATTCTTTACTTTCCTCCACATGATTATTACATCATTGTGTACATAATCAACCAATCACCGTCCATTTTAGCTACCTTTCTCATCACTCACATCGTGGCATGTGATGATGTGATCAAATTACAAACTTGATTAAAGCTAATTTAGTTGCAAACTTGCAATGGAGTGTGTGTTTTTGCAACCTTTTCTTTGCAAATGTTGAAAAGATCTAGCAAAAGTGTGGAACACACCTTATATTTCCTCCTTTAAAAAAAAAAAATTACACATAAGAAATTATATAGTTATTTGGGATTTTGTTAGATTCGTCCACATATATATTTTCAATTTTTTTTATAACTTTAACTAATGAATAATTAAAGTATTAATGACTAAAGTTATGCATTTACAAGTGTGAAATAACAAATGATACAATTATAAAAAATGAGTCATTGTAATTCACCCATTCGAAAGTATGGTGTCAGGTAATCCGCCTAAGTTTTCTTTCCTTATCAAAAAAAAAAAAAAAAAAACCCAGAGTAGGTATTTTTTGTGTGTGTTAGCACTCAGTTCACCCTTAGGGGCTAATCCGGATTCGGGGCGAGTTTTGGGTGGATAGGTTTCAGTCCCCTTCCAATTATTGTTGCGGGGGATCGAACACGGATCCTTCCTACCAAATTTAGTCTCAATCACCACTGAACCAACAAAAAATTGGTAAAATCAAAGTAAGTATAAGTAGTTGCATGACTAGCATCTGGCAGAGTTGTTTTTTCCACTAAATTTAAGATATGTTGTGACTCATAAGTAGCTTTCAAAATATACTTCGTACATAGTACTTTTAGAAATCCCTACTTTTCTAGTAGCACAAAAGCGTGAAAGAAGGTCTAGCTATCAGTCAAAATTGTTATGCACTAATCCAATATTCCTAGTTTGCATACAATTCAAGTAGACATTTGAAGACTTAAGAGTGGAAAATAGAAGTGTTAACTTTTCAATTAAGGCGTTAATGGTGTCCAAGTCCATATAATATCACATTAGTTTATGCCAACGAATGTGGAAATACATGGCTTGACACCAAATTGTGGAATTCTAGAAGTTAGGGATAATTGGATAACAAGATTGTGTCTCCTTAAAGAAAAGGAGTAGCAAAAGTTGTGGGTCTATTTGCTCATTTGTCCCCTTTATTAATTGGTGTTGGTCATTACTCATTAGGTCATGGTCCTTGCAAAAATTGCTTTTAAGTCAATGTAATAAAAATTTAATCTTTAAAATTATTGTGATTCAGGAATATACAGTGGATACGGAAGTAAGAGCGGCACACAATGTGTTAGAAGCGTGTGCGCAGATTGACACAATTGACAAAGTTGTTTTCACGTCATCGGCAGCAACCATTCTCTGGAGAGAAAATCGCCAGATGATGTCAGAACTCGACGAGAGGCACTGGAGTGATGTGGGATATTGTCGTAAATTTAAGGTAAGAATAGTAATTTGACTATGAAAATTCAAAGTCAAACTCTAAAATGTGACTAAAGACTAATTTGAATAAATGTTATTCTATAGTTATGGCATGCATTGTCAAAGACTTTAGCCGAGAAGAGTGCATGGGCATTGGCAATGGACCGTGGGATTAGCATGGTAACAATCAACGGTGGATTGTTGGTGTCTGATCTTTCCATAAGACACCCTTACTTAAAAGGAGCGGCTGAGATGTACCAAGATGGAGTATTAGTGACCGTTGATTTGAACTTCTTGGTTGATGCCCACATTTGTGTTTTTGAAGACATTTCAACTTATGGACGATACTTATGCTTTAATCACGTCGTTAATCGCCCTGAAAGCGCGATTAAGCTCGCTAAGATCTTGTTGCCCTCGGCACCCGTACAAGCCCAAAGGTACATGACCTATAAACCCGAGATTAATTAGTTGTTACATTATCACAATTATTTCAATATTATACGGAGTATTTAGGTATGGATTTGAATATTTACATAGTGTTTTTGTACGCACAGTTTTCAAGAGGACGACAAAACGATTCAACAAAGGATTACCAACAAGAAGTTGAAGAAATTGATCATGGAATTTGAATCTCATCATGATGATCAAGATATCAACTTATAATTCAAAGTTTTACTTACCTTGAAAGCATAACAAAATACCAAAGCGCAAGCTGCTCCATTCTTTCGTTAAATAAAAGAAGTGTCATACAATATGTTGTAAATAAATATCTATACATAATTAGATATAGATAAATTATAACATGCGGCGACAAGATGATGATGATAATTAGCAATATATTATAAAAGGAGGTATCTTTGTACGTACTTGTAGATTTTAACACATACGTATATGTAGTAGTAGTTTTCATATGATATAAATGCGCAATAGTTGTAATGTAATATATCACATGATAGTTATATGTTGTACGGGGTATTATATCGTTGTTTATGATACACAAAATTATGTTGAACAAATCTTTAACGGGTCGGCCAAACCCTTGTGAATCTTACCCATGCTCCATCAACATATTTCAACTAGCACGCTCAGGTTTTAGCGCAAATGTTAAGCAAAGAAATGTTGATGCAGTACCTGGAGTATTTGGTGAAGTTAGAGCTTTTGTAAACGCCATGAGATATTACGAGTGTAGTAACGCC

mRNA sequence

CCAACTTCTTCTATTAAAACCCCATTATTATTTATTATTATTTTCATAACTATATAAATAAATAATACACTCTCTTCACTAACTTACTCTTACAATTTTCCCACCAACATAAACCACCAAAAATGGCCCCTGCATCATTCATCCCCATTTCAAACACAGTATGTGTTATGGATGCGTCCGGTTCACTCGGTTCGAGTCTCGTAAACCGGCTTCTTCAACGTGGTTACATTGTTCATGCCGCGGTTCAACCTCACGGTGAAGTGAAGGGATTGGAGGGGTTAAGTGATGATGATGGAAAAGAAAGGTTGAAGATATTTGATTGTGATGTGTTTGATTATCATAGTATACTTGATGTTTTGAAGGGTTGCTCTGCTTTGTTCTATAACTTTGAACTTCCTTTTGATCAACCTGTCTATGATGAATATACAGTGGATACGGAAGTAAGAGCGGCACACAATGTGTTAGAAGCGTGTGCGCAGATTGACACAATTGACAAAGTTGTTTTCACGTCATCGGCAGCAACCATTCTCTGGAGAGAAAATCGCCAGATGATGTCAGAACTCGACGAGAGGCACTGGAGTGATGTGGGATATTGTCGTAAATTTAAGTTATGGCATGCATTGTCAAAGACTTTAGCCGAGAAGAGTGCATGGGCATTGGCAATGGACCGTGGGATTAGCATGGTAACAATCAACGGTGGATTGTTGGTGTCTGATCTTTCCATAAGACACCCTTACTTAAAAGGAGCGGCTGAGATGTACCAAGATGGAGTATTAGTGACCGTTGATTTGAACTTCTTGGTTGATGCCCACATTTGTGTTTTTGAAGACATTTCAACTTATGGACGATACTTATGCTTTAATCACGTCGTTAATCGCCCTGAAAGCGCGATTAAGCTCGCTAAGATCTTGTTGCCCTCGGCACCCGTACAAGCCCAAAGTTTTCAAGAGGACGACAAAACGATTCAACAAAGGATTACCAACAAGAAGTTGAAGAAATTGATCATGGAATTTGAATCTCATCATGATGATCAAGATATCAACTTATAATTCAAAGTTTTACTTACCTTGAAAGCATAACAAAATACCAAAGCGCAAGCTGCTCCATTCTTTCGTTAAATAAAAGAAGTGTCATACAATATGTTGTAAATAAATATCTATACATAATTAGATATAGATAAATTATAACATGCGGCGACAAGATGATGATGATAATTAGCAATATATTATAAAAGGAGGTATCTTTGTACGTACTTGTAGATTTTAACACATACGTATATGTAGTAGTAGTTTTCATATGATATAAATGCGCAATAGTTGTAATGTAATATATCACATGATAGTTATATGTTGTACGGGGTATTATATCGTTGTTTATGATACACAAAATTATGTTGAACAAATCTTTAACGGGTCGGCCAAACCCTTGTGAATCTTACCCATGCTCCATCAACATATTTCAACTAGCACGCTCAGGTTTTAGCGCAAATGTTAAGCAAAGAAATGTTGATGCAGTACCTGGAGTATTTGGTGAAGTTAGAGCTTTTGTAAACGCCATGAGATATTACGAGTGTAGTAACGCC

Coding sequence (CDS)

ATGGCCCCTGCATCATTCATCCCCATTTCAAACACAGTATGTGTTATGGATGCGTCCGGTTCACTCGGTTCGAGTCTCGTAAACCGGCTTCTTCAACGTGGTTACATTGTTCATGCCGCGGTTCAACCTCACGGTGAAGTGAAGGGATTGGAGGGGTTAAGTGATGATGATGGAAAAGAAAGGTTGAAGATATTTGATTGTGATGTGTTTGATTATCATAGTATACTTGATGTTTTGAAGGGTTGCTCTGCTTTGTTCTATAACTTTGAACTTCCTTTTGATCAACCTGTCTATGATGAATATACAGTGGATACGGAAGTAAGAGCGGCACACAATGTGTTAGAAGCGTGTGCGCAGATTGACACAATTGACAAAGTTGTTTTCACGTCATCGGCAGCAACCATTCTCTGGAGAGAAAATCGCCAGATGATGTCAGAACTCGACGAGAGGCACTGGAGTGATGTGGGATATTGTCGTAAATTTAAGTTATGGCATGCATTGTCAAAGACTTTAGCCGAGAAGAGTGCATGGGCATTGGCAATGGACCGTGGGATTAGCATGGTAACAATCAACGGTGGATTGTTGGTGTCTGATCTTTCCATAAGACACCCTTACTTAAAAGGAGCGGCTGAGATGTACCAAGATGGAGTATTAGTGACCGTTGATTTGAACTTCTTGGTTGATGCCCACATTTGTGTTTTTGAAGACATTTCAACTTATGGACGATACTTATGCTTTAATCACGTCGTTAATCGCCCTGAAAGCGCGATTAAGCTCGCTAAGATCTTGTTGCCCTCGGCACCCGTACAAGCCCAAAGTTTTCAAGAGGACGACAAAACGATTCAACAAAGGATTACCAACAAGAAGTTGAAGAAATTGATCATGGAATTTGAATCTCATCATGATGATCAAGATATCAACTTATAA

Protein sequence

MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKERLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESHHDDQDINL

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo23280	Spo23280	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo23280.1	Spo23280.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo23280.1.utr5p.1	Spo23280.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo23280.1.CDS.5	Spo23280.1.CDS.5	CDS
Spo23280.1.CDS.4	Spo23280.1.CDS.4	CDS
Spo23280.1.CDS.3	Spo23280.1.CDS.3	CDS
Spo23280.1.CDS.2	Spo23280.1.CDS.2	CDS
Spo23280.1.CDS.1	Spo23280.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo23280.1.utr3p.1	Spo23280.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo23280.1.exon.5	Spo23280.1.exon.5	exon
Spo23280.1.exon.4	Spo23280.1.exon.4	exon
Spo23280.1.exon.3	Spo23280.1.exon.3	exon
Spo23280.1.exon.2	Spo23280.1.exon.2	exon
Spo23280.1.exon.1	Spo23280.1.exon.1	exon

Homology

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BLAST of Spo23280.1 vs. NCBI nr
Match: gi|902198573|gb|KNA13714.1| (hypothetical protein SOVF_113850 [Spinacia oleracea])

HSP 1 Score: 623.2 bits (1606), Expect = 2.400e-175
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60
           MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE
Sbjct: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60

Query: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120
           RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI
Sbjct: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120

Query: 121 DTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALA 180
           DTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALA
Sbjct: 121 DTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALA 180

Query: 181 MDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTY 240
           MDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTY
Sbjct: 181 MDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTY 240

Query: 241 GRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESH 300
           GRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESH
Sbjct: 241 GRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESH 300

Query: 301 HDDQDINL 309
           HDDQDINL
Sbjct: 301 HDDQDINL 308

[Close]

BLAST of Spo23280.1 vs. NCBI nr
Match: gi|731367341|ref|XP_010695556.1| (PREDICTED: tetraketide alpha-pyrone reductase 1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 521.5 bits (1342), Expect = 9.700e-145
Identity = 260/307 (84.69%), Postives = 283/307 (92.18%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60
           MAPAS+IPISNTVCVMDASGSLG +LV RLLQRGYIVHAAVQPHGEVKGL+GL DD GK+
Sbjct: 1   MAPASYIPISNTVCVMDASGSLGLNLVRRLLQRGYIVHAAVQPHGEVKGLKGLCDDHGKD 60

Query: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120
           RLKIFDCD+FDY +IL+ LKGCSALFYNF+LP DQP YDEYT D EVRAA NVLEACAQ+
Sbjct: 61  RLKIFDCDLFDYQTILEALKGCSALFYNFQLPHDQPEYDEYTADIEVRAAQNVLEACAQM 120

Query: 121 DTIDKVVFTSSAATILWRENRQ-MMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWAL 180
           D IDKV+FTSSA TILWRE+R+ M SELDERHWSDVG+CRKFKLWHALSKTLAEKSAWAL
Sbjct: 121 DMIDKVIFTSSATTILWREDRKAMSSELDERHWSDVGFCRKFKLWHALSKTLAEKSAWAL 180

Query: 181 AMDRGISMVTINGGLLV-SDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDIS 240
           AMDR ISMVTIN GLLV SDLSIRHPYLKGAAEMYQDGVLVTVDLNFL+DAHI VFEDIS
Sbjct: 181 AMDREISMVTINAGLLVGSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLLDAHIHVFEDIS 240

Query: 241 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFE 300
           TYGRYLCFN+V+NRPE+AIKLAKIL+PSA VQAQSFQED K IQQRI+NKKLK++++EFE
Sbjct: 241 TYGRYLCFNNVINRPENAIKLAKILMPSASVQAQSFQEDGKMIQQRISNKKLKEVLVEFE 300

Query: 301 SHHDDQD 306
           SH  DQD
Sbjct: 301 SHPVDQD 307

[Close]

BLAST of Spo23280.1 vs. NCBI nr
Match: gi|743809634|ref|XP_011018544.1| (PREDICTED: cinnamoyl-CoA reductase 1-like [Populus euphratica])

HSP 1 Score: 418.3 bits (1074), Expect = 1.200e-113
Identity = 210/301 (69.77%), Postives = 247/301 (82.06%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60
           MAPASF PISNTVCVMDASGSLG SLV RLLQRGY+VHAAVQ       L GLS ++ K 
Sbjct: 1   MAPASFNPISNTVCVMDASGSLGLSLVQRLLQRGYMVHAAVQNRNCELQLNGLSCENKK- 60

Query: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120
            LKIF  D FDY SI+D L+ CS LFY FE P DQP YDE   + EVRAAHNVLEACAQ 
Sbjct: 61  -LKIFYADPFDYKSIIDALRSCSGLFYTFEPPVDQPTYDESMTEVEVRAAHNVLEACAQT 120

Query: 121 DTIDKVVFTSSAATILWRENRQM-MSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWAL 180
           DTIDKVVFTSSA  ++WR++R+  +++LDERHWSD+ +CRKFKLWHALSKTLAEK+ WAL
Sbjct: 121 DTIDKVVFTSSATAVIWRDDRKSEIADLDERHWSDINFCRKFKLWHALSKTLAEKTTWAL 180

Query: 181 AMDRGISMVTINGGLLVS-DLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDIS 240
           AMDRG++MV++N GLLVS +LSI++PYL GAAE+Y+DGV VTVDLNFLVDAHIC++ED+S
Sbjct: 181 AMDRGVNMVSVNAGLLVSPELSIKNPYLTGAAELYEDGVFVTVDLNFLVDAHICIYEDVS 240

Query: 241 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFE 300
           +YGRYLCFNHVVNR E AI+LA +L PSAP   QS  +D K IQQRI+NKKL +L++ FE
Sbjct: 241 SYGRYLCFNHVVNRHEDAIELASMLTPSAPSLPQSSDQDVKIIQQRISNKKLNELMVGFE 299

[Close]

BLAST of Spo23280.1 vs. NCBI nr
Match: gi|629106629|gb|KCW71775.1| (hypothetical protein EUGRSUZ_E00270 [Eucalyptus grandis])

HSP 1 Score: 417.5 bits (1072), Expect = 2.000e-113
Identity = 207/302 (68.54%), Postives = 244/302 (80.79%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDD--DG 60
           MAPAS     N VCVMDASG LGS LV RLL+RGY VHAAVQ  GE+  L+ LS    D 
Sbjct: 56  MAPASLHEPPNAVCVMDASGRLGSRLVRRLLRRGYSVHAAVQSEGEIDRLKELSSSSPDD 115

Query: 61  KERLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACA 120
            ERLKIF  D FDY SI+D L+GCS LFY FE P DQP YDEY  + EVRAAHNVLEACA
Sbjct: 116 AERLKIFRSDPFDYQSIVDALRGCSGLFYAFEPPQDQPSYDEYMTEVEVRAAHNVLEACA 175

Query: 121 QIDTIDKVVFTSSAATILWRENRQMM-SELDERHWSDVGYCRKFKLWHALSKTLAEKSAW 180
           Q DTIDKVVFTSS   ++W ++R+   S+ DERHWS+V  CRK+KLWHALSKTLAEK+AW
Sbjct: 176 QTDTIDKVVFTSSVTAVVWTDDRKSQESDFDERHWSNVNLCRKYKLWHALSKTLAEKAAW 235

Query: 181 ALAMDRGISMVTINGGLLVS-DLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFED 240
           ALAMDRG++MV++NGGLL+  DLSI HPYLKGAAEMY+DGVLVTVD++FLVDAH+CVFED
Sbjct: 236 ALAMDRGVNMVSVNGGLLMGPDLSIAHPYLKGAAEMYEDGVLVTVDVDFLVDAHLCVFED 295

Query: 241 ISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIME 299
           +S+YGRYLCFNH+VNRPE A+KLA++L PSAP   QS+ ++ K I+QRI NKKL KL+++
Sbjct: 296 VSSYGRYLCFNHIVNRPEDALKLAQMLTPSAPSMPQSYDQELKIIEQRIGNKKLNKLMVD 355

[Close]

BLAST of Spo23280.1 vs. NCBI nr
Match: gi|702335176|ref|XP_010055308.1| (PREDICTED: tetraketide alpha-pyrone reductase 1-like isoform X2 [Eucalyptus grandis])

HSP 1 Score: 417.5 bits (1072), Expect = 2.000e-113
Identity = 207/302 (68.54%), Postives = 244/302 (80.79%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDD--DG 60
           MAPAS     N VCVMDASG LGS LV RLL+RGY VHAAVQ  GE+  L+ LS    D 
Sbjct: 1   MAPASLHEPPNAVCVMDASGRLGSRLVRRLLRRGYSVHAAVQSEGEIDRLKELSSSSPDD 60

Query: 61  KERLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACA 120
            ERLKIF  D FDY SI+D L+GCS LFY FE P DQP YDEY  + EVRAAHNVLEACA
Sbjct: 61  AERLKIFRSDPFDYQSIVDALRGCSGLFYAFEPPQDQPSYDEYMTEVEVRAAHNVLEACA 120

Query: 121 QIDTIDKVVFTSSAATILWRENRQMM-SELDERHWSDVGYCRKFKLWHALSKTLAEKSAW 180
           Q DTIDKVVFTSS   ++W ++R+   S+ DERHWS+V  CRK+KLWHALSKTLAEK+AW
Sbjct: 121 QTDTIDKVVFTSSVTAVVWTDDRKSQESDFDERHWSNVNLCRKYKLWHALSKTLAEKAAW 180

Query: 181 ALAMDRGISMVTINGGLLVS-DLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFED 240
           ALAMDRG++MV++NGGLL+  DLSI HPYLKGAAEMY+DGVLVTVD++FLVDAH+CVFED
Sbjct: 181 ALAMDRGVNMVSVNGGLLMGPDLSIAHPYLKGAAEMYEDGVLVTVDVDFLVDAHLCVFED 240

Query: 241 ISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIME 299
           +S+YGRYLCFNH+VNRPE A+KLA++L PSAP   QS+ ++ K I+QRI NKKL KL+++
Sbjct: 241 VSSYGRYLCFNHIVNRPEDALKLAQMLTPSAPSMPQSYDQELKIIEQRIGNKKLNKLMVD 300

[Close]

BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R450_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_113850 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 1.600e-175
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60
           MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE
Sbjct: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60

Query: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120
           RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI
Sbjct: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120

Query: 121 DTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALA 180
           DTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALA
Sbjct: 121 DTIDKVVFTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALA 180

Query: 181 MDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTY 240
           MDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTY
Sbjct: 181 MDRGISMVTINGGLLVSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTY 240

Query: 241 GRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESH 300
           GRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESH
Sbjct: 241 GRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFESH 300

Query: 301 HDDQDINL 309
           HDDQDINL
Sbjct: 301 HDDQDINL 308

[Close]

BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B9H1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g125610 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 6.700e-145
Identity = 260/307 (84.69%), Postives = 283/307 (92.18%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60
           MAPAS+IPISNTVCVMDASGSLG +LV RLLQRGYIVHAAVQPHGEVKGL+GL DD GK+
Sbjct: 1   MAPASYIPISNTVCVMDASGSLGLNLVRRLLQRGYIVHAAVQPHGEVKGLKGLCDDHGKD 60

Query: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120
           RLKIFDCD+FDY +IL+ LKGCSALFYNF+LP DQP YDEYT D EVRAA NVLEACAQ+
Sbjct: 61  RLKIFDCDLFDYQTILEALKGCSALFYNFQLPHDQPEYDEYTADIEVRAAQNVLEACAQM 120

Query: 121 DTIDKVVFTSSAATILWRENRQ-MMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWAL 180
           D IDKV+FTSSA TILWRE+R+ M SELDERHWSDVG+CRKFKLWHALSKTLAEKSAWAL
Sbjct: 121 DMIDKVIFTSSATTILWREDRKAMSSELDERHWSDVGFCRKFKLWHALSKTLAEKSAWAL 180

Query: 181 AMDRGISMVTINGGLLV-SDLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDIS 240
           AMDR ISMVTIN GLLV SDLSIRHPYLKGAAEMYQDGVLVTVDLNFL+DAHI VFEDIS
Sbjct: 181 AMDREISMVTINAGLLVGSDLSIRHPYLKGAAEMYQDGVLVTVDLNFLLDAHIHVFEDIS 240

Query: 241 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFE 300
           TYGRYLCFN+V+NRPE+AIKLAKIL+PSA VQAQSFQED K IQQRI+NKKLK++++EFE
Sbjct: 241 TYGRYLCFNNVINRPENAIKLAKILMPSASVQAQSFQEDGKMIQQRISNKKLKEVLVEFE 300

Query: 301 SHHDDQD 306
           SH  DQD
Sbjct: 301 SHPVDQD 307

[Close]

BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
Match: A0A059C1I6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E00270 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.400e-113
Identity = 207/302 (68.54%), Postives = 244/302 (80.79%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDD--DG 60
           MAPAS     N VCVMDASG LGS LV RLL+RGY VHAAVQ  GE+  L+ LS    D 
Sbjct: 56  MAPASLHEPPNAVCVMDASGRLGSRLVRRLLRRGYSVHAAVQSEGEIDRLKELSSSSPDD 115

Query: 61  KERLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACA 120
            ERLKIF  D FDY SI+D L+GCS LFY FE P DQP YDEY  + EVRAAHNVLEACA
Sbjct: 116 AERLKIFRSDPFDYQSIVDALRGCSGLFYAFEPPQDQPSYDEYMTEVEVRAAHNVLEACA 175

Query: 121 QIDTIDKVVFTSSAATILWRENRQMM-SELDERHWSDVGYCRKFKLWHALSKTLAEKSAW 180
           Q DTIDKVVFTSS   ++W ++R+   S+ DERHWS+V  CRK+KLWHALSKTLAEK+AW
Sbjct: 176 QTDTIDKVVFTSSVTAVVWTDDRKSQESDFDERHWSNVNLCRKYKLWHALSKTLAEKAAW 235

Query: 181 ALAMDRGISMVTINGGLLVS-DLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFED 240
           ALAMDRG++MV++NGGLL+  DLSI HPYLKGAAEMY+DGVLVTVD++FLVDAH+CVFED
Sbjct: 236 ALAMDRGVNMVSVNGGLLMGPDLSIAHPYLKGAAEMYEDGVLVTVDVDFLVDAHLCVFED 295

Query: 241 ISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIME 299
           +S+YGRYLCFNH+VNRPE A+KLA++L PSAP   QS+ ++ K I+QRI NKKL KL+++
Sbjct: 296 VSSYGRYLCFNHIVNRPEDALKLAQMLTPSAPSMPQSYDQELKIIEQRIGNKKLNKLMVD 355

[Close]

BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
Match: B9MU38_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s02320g PE=3 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.800e-113
Identity = 207/301 (68.77%), Postives = 247/301 (82.06%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKE 60
           MAPASF P+SNTVCVMDASG+LG SLV RLLQRGY+VHAAVQ H     L GLS ++ K 
Sbjct: 1   MAPASFNPVSNTVCVMDASGNLGLSLVQRLLQRGYMVHAAVQNHNCELQLNGLSCENKK- 60

Query: 61  RLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQI 120
            LKIF  D FDY SILD L+ CS LFY FE P DQP YDE   + EVRAAHNVLEACA  
Sbjct: 61  -LKIFYADPFDYKSILDALRSCSGLFYTFEPPEDQPTYDESMTEVEVRAAHNVLEACAHT 120

Query: 121 DTIDKVVFTSSAATILWRENRQM-MSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWAL 180
           +T+DKVVFTSSA  ++WR++R+  +++ DERHWSD+ +CRKFKLWHALSKTLAEK+ WAL
Sbjct: 121 ETMDKVVFTSSATAVIWRDDRKSEIADFDERHWSDINFCRKFKLWHALSKTLAEKTTWAL 180

Query: 181 AMDRGISMVTINGGLLVS-DLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDIS 240
           AMDRG++MV++N GLLVS DLSI++PYL GAAE+Y+DGV VTVDLNFLVDAHIC++ED+S
Sbjct: 181 AMDRGVNMVSVNAGLLVSPDLSIKNPYLTGAAELYEDGVFVTVDLNFLVDAHICIYEDVS 240

Query: 241 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFE 300
           +YGRYLCFNHVVNR E AI+LA +L P+AP   QSF +D K IQQRI+NKKL +L++ FE
Sbjct: 241 SYGRYLCFNHVVNRHEDAIELASMLTPAAPSLPQSFDQDVKIIQQRISNKKLNELMVGFE 299

[Close]

BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
Match: A0A059C0L4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E00270 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 9.800e-112
Identity = 207/307 (67.43%), Postives = 244/307 (79.48%), Query Frame = 1

		  
Query: 1   MAPASFIPISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDD--DG 60
           MAPAS     N VCVMDASG LGS LV RLL+RGY VHAAVQ  GE+  L+ LS    D 
Sbjct: 56  MAPASLHEPPNAVCVMDASGRLGSRLVRRLLRRGYSVHAAVQSEGEIDRLKELSSSSPDD 115

Query: 61  KERLKIFDCDVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACA 120
            ERLKIF  D FDY SI+D L+GCS LFY FE P DQP YDEY  + EVRAAHNVLEACA
Sbjct: 116 AERLKIFRSDPFDYQSIVDALRGCSGLFYAFEPPQDQPSYDEYMTEVEVRAAHNVLEACA 175

Query: 121 QIDTIDKVVFTSSAATILWRENRQMM-SELDERHWSDVGYCRKFKLWHALSKTLAEKSAW 180
           Q DTIDKVVFTSS   ++W ++R+   S+ DERHWS+V  CRK+KLWHALSKTLAEK+AW
Sbjct: 176 QTDTIDKVVFTSSVTAVVWTDDRKSQESDFDERHWSNVNLCRKYKLWHALSKTLAEKAAW 235

Query: 181 ALAMDRGISMVTINGGLLVS-DLSIRHPYLKGAAEMYQDGVLVTVDLNFLVDAHICVFED 240
           ALAMDRG++MV++NGGLL+  DLSI HPYLKGAAEMY+DGVLVTVD++FLVDAH+CVFED
Sbjct: 236 ALAMDRGVNMVSVNGGLLMGPDLSIAHPYLKGAAEMYEDGVLVTVDVDFLVDAHLCVFED 295

Query: 241 ISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQ-----SFQEDDKTIQQRITNKKLK 299
           +S+YGRYLCFNH+VNRPE A+KLA++L PSAP   Q     S+ ++ K I+QRI NKKL 
Sbjct: 296 VSSYGRYLCFNHIVNRPEDALKLAQMLTPSAPSMPQSMVACSYDQELKIIEQRIGNKKLN 355

[Close]

BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
Match: CCR1_ARATH (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana GN=CCR1 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.900e-34
Identity = 97/302 (32.12%), Postives = 150/302 (49.67%), Query Frame = 1

		  
Query: 8   PISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKERLKIFDC 67
           P   TVCV  A G + S +V  LL+RGY V   V+   + K       + GKERL +   
Sbjct: 8   PAGKTVCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDPKNTHLRELEGGKERLILCKA 67

Query: 68  DVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVV 127
           D+ DY ++   + GC  +F+      D P   E  V+  V  A  V+ A A+   + +VV
Sbjct: 68  DLQDYEALKAAIDGCDGVFHTASPVTDDP---EQMVEPAVNGAKFVINAAAEAK-VKRVV 127

Query: 128 FTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISM 187
            TSS   +    NR   + +DE  WSD+ +C+  K W+   K +AE++AW  A ++G+ +
Sbjct: 128 ITSSIGAVYMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGVDL 187

Query: 188 VTINGGLLVS-------DLSIRH--PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDIS 247
           V +N  L++        + S+ H   YL G+A+ Y +     VD+  +  AH+ V+E  S
Sbjct: 188 VVLNPVLVLGPPLQPTINASLYHVLKYLTGSAKTYANLTQAYVDVRDVALAHVLVYEAPS 247

Query: 248 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQE-DDKTIQQRITNKKLKKLIMEF 300
             GRYL      +R E    LAK L P  P+  +   E + +    + TN+K+K L +EF
Sbjct: 248 ASGRYLLAESARHRGEVVEILAK-LFPEYPLPTKCKDEKNPRAKPYKFTNQKIKDLGLEF 304

[Close]

BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
Match: CCR2_ARATH (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana GN=CCR2 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.300e-32
Identity = 92/296 (31.08%), Postives = 148/296 (50.00%), Query Frame = 1

		  
Query: 13  VCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKERLKIFDCDVFDY 72
           VCV  A G + S +V  LL+RGY V   V+   + K          KERL +   D+ DY
Sbjct: 8   VCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDPKNNHLRELQGAKERLTLHSADLLDY 67

Query: 73  HSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVVFTSSA 132
            ++   + GC  +F+      D P   E  ++  V  A  V++A A+   + +VVFTSS 
Sbjct: 68  EALCATIDGCDGVFHTASPMTDDP---ETMLEPAVNGAKFVIDAAAKAK-VKRVVFTSSI 127

Query: 133 ATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISMVTING 192
             +    NR   + +DE  WSD+ +C+  K W+   K LAE+SAW  A  +G+ +V +N 
Sbjct: 128 GAVYMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDLVVLNP 187

Query: 193 GLLVS-------DLSIRH--PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTYGRY 252
            L++        + S+ H   YL G+A+ Y +   V VD+  +   H+ V+E  S  GRY
Sbjct: 188 VLVLGPPLQSAINASLVHILKYLTGSAKTYANLTQVYVDVRDVALGHVLVYEAPSASGRY 247

Query: 253 LCFNHVVNRPESAIKLAKILLPSAPVQAQ-SFQEDDKTIQQRITNKKLKKLIMEFE 299
           +     ++R E    LAK   P  P+  + S +++ +    + T +K+K L +EF+
Sbjct: 248 ILAETALHRGEVVEILAK-FFPEYPLPTKCSDEKNPRAKPYKFTTQKIKDLGLEFK 298

[Close]

BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
Match: TKPR1_ARATH (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.500e-32
Identity = 97/301 (32.23%), Postives = 152/301 (50.50%), Query Frame = 1

		  
Query: 13  VCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDG-KERLKIFDCDVFD 72
           VCV  ASG L S LV RLL  GY V   V+  G  K L  L   +G KERL++   D+ +
Sbjct: 8   VCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGNEKKLAHLWKLEGAKERLRLVKADLME 67

Query: 73  YHSILDVLKGCSALFYNFE---LPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVVF 132
             S  + + GC  +F+       P   P  +E  +   +    NVL +C +  ++ +VV 
Sbjct: 68  EGSFDNAIMGCQGVFHTASPVLKPTSNP--EEEILRPAIEGTLNVLRSCRKNPSLKRVVL 127

Query: 133 TSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISMV 192
           TSS++T+  R++      LDE  W+ V  C++F++W+ALSKTLAE++AW  + + GI +V
Sbjct: 128 TSSSSTVRIRDDFDPKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKFSEENGIDLV 187

Query: 193 TINGGLLV---------SDLSIRHPYLKGAAEMYQ-DGVLVTVDLNFLVDAHICVFEDIS 252
           T+    LV         S  S     LKG  E +Q  G +  V ++ +   HI VFE  +
Sbjct: 188 TVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVARTHIVVFEHEA 247

Query: 253 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLIMEFE 300
             GRY+C ++V++  E  +       PS P+  +   E    +       K++ L ++F+
Sbjct: 248 AQGRYICSSNVISL-EELVSFLSARYPSLPIPKRF--EKLNRLHYDFDTSKIQSLGLKFK 303

[Close]

BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
Match: DFRA_MALDO (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica GN=DFR PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.300e-28
Identity = 92/309 (29.77%), Postives = 160/309 (51.78%), Query Frame = 1

		  
Query: 10  SNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSD-DDGKERLKIFDCD 69
           S +VCV  ASG +GS LV RLL+ GY V A V+     K ++ L D    +  L ++  D
Sbjct: 5   SESVCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKAD 64

Query: 70  VFDYHSILDVLKGCSALFY-NFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVV 129
           + D  S  + ++GCS +F+    + F+    +   +   +    ++L+AC +  T+ K+V
Sbjct: 65  LADEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLV 124

Query: 130 FTSSAATILWRENRQMMSELDERHWSDVGYCRKFKL--W-HALSKTLAEKSAWALAMDRG 189
           FTSSA T+   E+++ +   DE +WSDV +CR  K+  W + +SKTLAE++AW  A +  
Sbjct: 125 FTSSAGTVNVEEHQKPV--YDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENN 184

Query: 190 ISMVTINGGLLVSDL---SIRHPYLKGAAEMYQD----GVLVT---VDLNFLVDAHICVF 249
           I  +TI   L++      S+    + G + + ++    G++     V L+ L  +HI ++
Sbjct: 185 IDFITIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLY 244

Query: 250 EDISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLI 304
           E     GRY+C +H     E  +K+ +   P   +  +    DD       ++KKL+++ 
Sbjct: 245 EHPKAEGRYICSSHDATIHE-LVKMLREKYPEYNIPTKFKGIDDNLEPVHFSSKKLREIG 304

[Close]

BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
Match: DFRA_PYRCO (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Pyrus communis GN=DFR PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.700e-27
Identity = 91/309 (29.45%), Postives = 160/309 (51.78%), Query Frame = 1

		  
Query: 10  SNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSD-DDGKERLKIFDCD 69
           S +VCV  ASG +GS LV RLL+ GY V A V+     K ++ L D    +  L ++  D
Sbjct: 5   SESVCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKAD 64

Query: 70  VFDYHSILDVLKGCSALFY-NFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVV 129
           + D  S  + ++GCS +F+    + F+    +   +   +    ++L+AC +  T+ K+V
Sbjct: 65  LADEGSFDEAIQGCSGVFHVATPMDFESRDPENEVIKPTINGLLDILKACQKAKTVRKLV 124

Query: 130 FTSSAATILWRENRQMMSELDERHWSDVGYCRKFKL--W-HALSKTLAEKSAWALAMDRG 189
           FTSSA T+   E+++ +   DE +WSDV +CR  K+  W + +SKTLAE++AW  A +  
Sbjct: 125 FTSSAGTVNVEEHQKPV--YDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENN 184

Query: 190 ISMVTINGGLLVSDL---SIRHPYLKGAAEMYQD----GVLVT---VDLNFLVDAHICVF 249
           I  +TI   L++      S+    + G + + ++    G++     V L+ L  +HI ++
Sbjct: 185 IDFITIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLY 244

Query: 250 EDISTYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQEDDKTIQQRITNKKLKKLI 304
           +     GRY+C +H     E  +K+ +   P   +  +    DD       ++KKL+++ 
Sbjct: 245 KHPKAEGRYICSSHDATIHE-LVKMLREKYPEYNIPTKFKGIDDNLEPVHFSSKKLREIG 304

[Close]

BLAST of Spo23280.1 vs. TAIR (Arabidopsis)
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 256.9 bits (655), Expect = 1.600e-68
Identity = 134/290 (46.21%), Postives = 191/290 (65.86%), Query Frame = 1

		  
Query: 14  CVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSD-DDGKERLKIFDCDVFDY 73
           CV+DAS  +G  ++ RLL RGY VHAA++ +GE    E + D +  +ERL+++D DV DY
Sbjct: 11  CVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEVYDVDVLDY 70

Query: 74  HSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVVFTSSA 133
            SIL  L  C+A+F   + P  +   +E  VD EVR A NV+EACA+ ++I+K+VF+SS 
Sbjct: 71  QSILISLNNCNAVFCCLDNP--EGYDEEKEVDLEVRGAINVVEACARTESIEKIVFSSSL 130

Query: 134 ATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISMVTING 193
              +WR+N     ++DE+ WSD+ +C K KLWHAL+KT +EK+AWALAMDR ++MV++N 
Sbjct: 131 TAAIWRDNIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAAWALAMDRMVNMVSVNP 190

Query: 194 GLLVSDLSIRH------PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTYGRYLCF 253
           GL+V     +H       YLKGAA+MY++GVL  VD+ F+ D HI  FED S  GRY CF
Sbjct: 191 GLIVGPSVAQHNPRPTMSYLKGAAQMYENGVLAYVDVEFVADVHIRAFEDTSACGRYFCF 250

Query: 254 NHVVNRPESAIKLAKILLPSAPVQAQSFQE--DDKTIQQRITNKKLKKLI 295
           N +VN  E A+KL + L P  P+  +  +E    +  ++R+ NKKL KL+
Sbjct: 251 NQIVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLRNKKLNKLV 298

[Close]

BLAST of Spo23280.1 vs. TAIR (Arabidopsis)
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 254.2 bits (648), Expect = 1.000e-67
Identity = 133/290 (45.86%), Postives = 189/290 (65.17%), Query Frame = 1

		  
Query: 14  CVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSD-DDGKERLKIFDCDVFDY 73
           CV+DAS  +G  ++ +LL RGY VHAA++ +GE +  E + + +  +ERL ++D DV DY
Sbjct: 11  CVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEERLVVYDVDVLDY 70

Query: 74  HSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVVFTSSA 133
            SIL  LK C+ +F   + P     YDE  VD EVR A NV+EAC + ++I+K+VF+SS 
Sbjct: 71  QSILVSLKTCNVVFCCLDSPEG---YDEKEVDLEVRGAINVVEACGRTESIEKIVFSSSL 130

Query: 134 ATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISMVTING 193
              +WR+N     ++DE+ WSD  +CR  KLWHAL+K L+EK+AWALAMDR ++MV+IN 
Sbjct: 131 TASIWRDNIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKAAWALAMDRRLNMVSINP 190

Query: 194 GLLVSDLSIRH------PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTYGRYLCF 253
           GL+V     +H       YLKGAA+MY++GVL  VD+ FL D HI  FED+S  GRY CF
Sbjct: 191 GLVVGPSVAQHNARPTMSYLKGAAQMYENGVLAYVDVKFLADVHIRAFEDVSACGRYFCF 250

Query: 254 NHVVNRPESAIKLAKILLPSAPV--QAQSFQEDDKTIQQRITNKKLKKLI 295
           N +VN  E A+KL + L P  P+  + ++     +  ++R+ N KL KL+
Sbjct: 251 NQIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERLRNNKLSKLV 297

[Close]

BLAST of Spo23280.1 vs. TAIR (Arabidopsis)
Match: AT1G15950.1 (cinnamoyl coa reductase 1)

HSP 1 Score: 146.0 bits (367), Expect = 3.900e-35
Identity = 97/302 (32.12%), Postives = 150/302 (49.67%), Query Frame = 1

		  
Query: 8   PISNTVCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKERLKIFDC 67
           P   TVCV  A G + S +V  LL+RGY V   V+   + K       + GKERL +   
Sbjct: 8   PAGKTVCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDPKNTHLRELEGGKERLILCKA 67

Query: 68  DVFDYHSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVV 127
           D+ DY ++   + GC  +F+      D P   E  V+  V  A  V+ A A+   + +VV
Sbjct: 68  DLQDYEALKAAIDGCDGVFHTASPVTDDP---EQMVEPAVNGAKFVINAAAEAK-VKRVV 127

Query: 128 FTSSAATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISM 187
            TSS   +    NR   + +DE  WSD+ +C+  K W+   K +AE++AW  A ++G+ +
Sbjct: 128 ITSSIGAVYMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGVDL 187

Query: 188 VTINGGLLVS-------DLSIRH--PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDIS 247
           V +N  L++        + S+ H   YL G+A+ Y +     VD+  +  AH+ V+E  S
Sbjct: 188 VVLNPVLVLGPPLQPTINASLYHVLKYLTGSAKTYANLTQAYVDVRDVALAHVLVYEAPS 247

Query: 248 TYGRYLCFNHVVNRPESAIKLAKILLPSAPVQAQSFQE-DDKTIQQRITNKKLKKLIMEF 300
             GRYL      +R E    LAK L P  P+  +   E + +    + TN+K+K L +EF
Sbjct: 248 ASGRYLLAESARHRGEVVEILAK-LFPEYPLPTKCKDEKNPRAKPYKFTNQKIKDLGLEF 304

[Close]

BLAST of Spo23280.1 vs. TAIR (Arabidopsis)
Match: AT1G80820.1 (cinnamoyl coa reductase)

HSP 1 Score: 141.7 bits (356), Expect = 7.400e-34
Identity = 92/296 (31.08%), Postives = 148/296 (50.00%), Query Frame = 1

		  
Query: 13  VCVMDASGSLGSSLVNRLLQRGYIVHAAVQPHGEVKGLEGLSDDDGKERLKIFDCDVFDY 72
           VCV  A G + S +V  LL+RGY V   V+   + K          KERL +   D+ DY
Sbjct: 8   VCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDPKNNHLRELQGAKERLTLHSADLLDY 67

Query: 73  HSILDVLKGCSALFYNFELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKVVFTSSA 132
            ++   + GC  +F+      D P   E  ++  V  A  V++A A+   + +VVFTSS 
Sbjct: 68  EALCATIDGCDGVFHTASPMTDDP---ETMLEPAVNGAKFVIDAAAKAK-VKRVVFTSSI 127

Query: 133 ATILWRENRQMMSELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDRGISMVTING 192
             +    NR   + +DE  WSD+ +C+  K W+   K LAE+SAW  A  +G+ +V +N 
Sbjct: 128 GAVYMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDLVVLNP 187

Query: 193 GLLVS-------DLSIRH--PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDISTYGRY 252
            L++        + S+ H   YL G+A+ Y +   V VD+  +   H+ V+E  S  GRY
Sbjct: 188 VLVLGPPLQSAINASLVHILKYLTGSAKTYANLTQVYVDVRDVALGHVLVYEAPSASGRY 247

Query: 253 LCFNHVVNRPESAIKLAKILLPSAPVQAQ-SFQEDDKTIQQRITNKKLKKLIMEFE 299
           +     ++R E    LAK   P  P+  + S +++ +    + T +K+K L +EF+
Sbjct: 248 ILAETALHRGEVVEILAK-FFPEYPLPTKCSDEKNPRAKPYKFTTQKIKDLGLEFK 298

[Close]

BLAST of Spo23280.1 vs. TAIR (Arabidopsis)
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 139.8 bits (351), Expect = 2.800e-33
Identity = 100/304 (32.89%), Postives = 156/304 (51.32%), Query Frame = 1

		  
Query: 13  VCVMDASGSLGSSLVNRLLQRGYIVHAAVQ-PHGEVKGLEGLSDDDGKERLKIFDCDVF- 72
           VCV      LG ++V RLL  GY V   V  P  + K  E  +D +      +    V  
Sbjct: 55  VCVTGGVSYLGRAIVKRLLVHGYSVRIVVDCPEDKEKVSEMEADAETASFSNMITSVVSR 114

Query: 73  --DYHSILDVLKGCSALFYN--FELPFDQPVYDEYTVDTEVRAAHNVLEACAQIDTIDKV 132
             +  S++    GC+ +F+   F  P     Y +   + E + + +V+EAC +  ++ K 
Sbjct: 115 LTEIDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEACTRTASVRKC 174

Query: 133 VFTSSAATILWRENRQMM---SELDERHWSDVGYCRKFKLWHALSKTLAEKSAWALAMDR 192
           VFTSS     W++N       S ++E  WSD   C   KLW+AL K  AEK+AW +A  +
Sbjct: 175 VFTSSLLACAWQKNPCNSLDHSVINEESWSDEQLCIDNKLWYALGKLKAEKAAWRIADSK 234

Query: 193 GISMVTINGGLLVS-DLSIRH-----PYLKGAAEMYQDGVLVTVDLNFLVDAHICVFEDI 252
           G+ + TI   L+   D   R+      YLKGA EMY +G+L T+D+N L  AH+C++E +
Sbjct: 235 GLKLATICPALITGPDFFNRNSTSTLAYLKGAKEMYSNGLLATMDVNRLAKAHVCLWEGL 294

Query: 253 ---STYGRYLCFNHVVNRPESAIKLAK-ILLPSAPVQAQSFQEDDKTIQQ---RITNKKL 295
              + +GRY+CF+ +++R + A KLAK I +    +   S   D  T  +   +I++KKL
Sbjct: 295 GNKTAFGRYICFDTILSR-DGAEKLAKDIDVQIEKICGNSNDSDANTETEASLQISDKKL 354

The following BLAST results are available for this feature:

BLAST of Spo23280.1 vs. NCBI nr
BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
BLAST of Spo23280.1 vs. TAIR (Arabidopsis)

BLAST of Spo23280.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902198573\|gb\|KNA13714.1\|	2.4e-175	100.	hypothetical protein SOVF_1138...	[more]
gi\|731367341\|ref\|XP_010695556.1\|	9.7e-145	84.6	PREDICTED: tetraketide alpha-p...	[more]
gi\|743809634\|ref\|XP_011018544.1\|	1.2e-113	69.7	PREDICTED: cinnamoyl-CoA reduc...	[more]
gi\|629106629\|gb\|KCW71775.1\|	2.0e-113	68.5	hypothetical protein EUGRSUZ_E...	[more]
gi\|702335176\|ref\|XP_010055308.1\|	2.0e-113	68.5	PREDICTED: tetraketide alpha-p...	[more]

BLAST of Spo23280.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9R450_SPIOL	1.6e-175	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8B9H1_BETVU	6.7e-145	84.6	Uncharacterized protein OS=Bet...	[more]
A0A059C1I6_EUCGR	1.4e-113	68.5	Uncharacterized protein OS=Euc...	[more]
B9MU38_POPTR	1.8e-113	68.7	Uncharacterized protein OS=Pop...	[more]
A0A059C0L4_EUCGR	9.8e-112	67.4	Uncharacterized protein OS=Euc...	[more]

BLAST of Spo23280.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
CCR1_ARATH	6.9e-34	32.1	Cinnamoyl-CoA reductase 1 OS=A...	[more]
CCR2_ARATH	1.3e-32	31.0	Cinnamoyl-CoA reductase 2 OS=A...	[more]
TKPR1_ARATH	8.5e-32	32.2	Tetraketide alpha-pyrone reduc...	[more]
DFRA_MALDO	4.3e-28	29.7	Bifunctional dihydroflavonol 4...	[more]
DFRA_PYRCO	1.7e-27	29.4	Bifunctional dihydroflavonol 4...	[more]

BLAST of Spo23280.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
AT2G23910.1	1.6e-68	46.2	NAD(P)-binding Rossmann-fold s...	[more]
AT4G30470.1	1.0e-67	45.8	NAD(P)-binding Rossmann-fold s...	[more]
AT1G15950.1	3.9e-35	32.1	cinnamoyl coa reductase 1	[more]
AT1G80820.1	7.4e-34	31.0	cinnamoyl coa reductase	[more]
AT5G14700.1	2.8e-33	32.8	NAD(P)-binding Rossmann-fold s...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002225	3-beta hydroxysteroid dehydrogenase/isomerase	PFAM	PF01073	3Beta_HSD	coord: 15..175 score: 3.
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720		coord: 12..294 score: 2.4
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 11..253 score: 2.46
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 12..298 score: 5.6E
None	No IPR available	PANTHER	PTHR10366:SF428	SUBFAMILY NOT NAMED	coord: 12..298 score: 5.6E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006694	steroid biosynthetic process
biological_process	GO:0008209	androgen metabolic process
biological_process	GO:0008207	C21-steroid hormone metabolic process
biological_process	GO:0008210	estrogen metabolic process
molecular_function	GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function	GO:0050662	coenzyme binding