Homology
BLAST of Spo25618.1 vs. NCBI nr
Match:
gi|902227189|gb|KNA20604.1| (hypothetical protein SOVF_050860 [Spinacia oleracea])
HSP 1 Score: 1349.0 bits (3490), Expect = 0.000e+0
Identity = 666/670 (99.40%), Postives = 668/670 (99.70%), Query Frame = 1
Query: 695 LTCLQELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLK 754
L+ +ELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLK
Sbjct: 178 LSLERELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLK 237
Query: 755 FSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIREC 814
FSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIREC
Sbjct: 238 FSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIREC 297
Query: 815 NNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWT 874
NNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWT
Sbjct: 298 NNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWT 357
Query: 875 RPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDE 934
RPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDE
Sbjct: 358 RPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDE 417
Query: 935 EEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVE 994
EEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVE
Sbjct: 418 EEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVE 477
Query: 995 DEDDIWVGDSASAAHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQLFPRLLKMDVRA 1054
DEDDIWVGDSASAAHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQLFPRLLKMDVRA
Sbjct: 478 DEDDIWVGDSASAAHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQLFPRLLKMDVRA 537
Query: 1055 CPKLMSMPLAPMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLE 1114
CPKLMSMPLAPMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLE
Sbjct: 538 CPKLMSMPLAPMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLE 597
Query: 1115 MRWIDDGLVSLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYC 1174
MRWIDDGLVSLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYC
Sbjct: 598 MRWIDDGLVSLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYC 657
Query: 1175 NRLENISGGNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEE 1234
NRLENISGGNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEE
Sbjct: 658 NRLENISGGNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEE 717
Query: 1235 KKEPGKITRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEM 1294
KKEPGKITRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEM
Sbjct: 718 KKEPGKITRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEM 777
Query: 1295 GNLSLLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYEKPDGEDCHLIQHIPNVK 1354
GNLSLLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYEKPDGEDCHLIQHIPNVK
Sbjct: 778 GNLSLLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYEKPDGEDCHLIQHIPNVK 837
Query: 1355 FYEKLIKYFD 1365
FYEKLIKYFD
Sbjct: 838 FYEKLIKYFD 847
BLAST of Spo25618.1 vs. NCBI nr
Match:
gi|731317068|ref|XP_010667614.1| (PREDICTED: putative disease resistance protein RGA3 [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 1085.5 bits (2806), Expect = 0.000e+0
Identity = 664/1376 (48.26%), Postives = 863/1376 (62.72%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MAEG++ L +KALE L+ +A +E SSWWGAR DLKKL ++ MIQARVRDAE+ QE E
Sbjct: 1 MAEGVISNLVAKALELLAASAFKEASSWWGARDDLKKLGKSMEMIQARVRDAEKYQEEEG 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKVFSRSG-PIFNIKLAHKV 120
+ ++ LWL RLK VLY+ADDLFD I+T+DR+ R +++ RS P+ N K+A ++
Sbjct: 61 SHAVKLWLRRLKLVLYQADDLFDHILTVDRQKQRRKQNHEVHFSLRSSSPVLNWKIAGEI 120
Query: 121 NSIREELNDIKSDMDVLNLKFFPAEELP-PLMMRLIKERQTSSFVKTEDIIGRENDTKLI 180
SIRE+L+DIKSD+ LNL + P P M L+ R T+ FV+ ED+IGRE D I
Sbjct: 121 KSIREQLDDIKSDIAGLNLPSYQHRVGPSPQMALLLNNRATTPFVEAEDVIGRECDKDKI 180
Query: 181 IGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPE 240
+ MLFDPK + E +++IPIVGFGGLGKTTLAQ +D+ VQ HF W VPE
Sbjct: 181 VEMLFDPKYD------AEKVTIIPIVGFGGLGKTTLAQFALSDQRVQNHFFPLKWAYVPE 240
Query: 241 VDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLDL 300
+D++AVM +++ +FT K L + +++ GIR+SI N+YLLVLDDIWDES DR +DL
Sbjct: 241 KNDRNAVMGKIFNSFTGRKYHN-LQMKDLEDGIRESIKGNRYLLVLDDIWDESGDRLIDL 300
Query: 301 LRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERN 360
L +CG GSKVIVTTRS VA +GT + Y+LG LT+++SWNLF++ A + QE +
Sbjct: 301 GNLFKCGEPGSKVIVTTRSDVVAKSLGTIKQPYRLGTLTDDQSWNLFERLALKPRQEGSS 360
Query: 361 -PDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAV 420
L+ IGK+IV CGNVPLAI+VVG L S+ + +EW +R++ SKA+ G +M +
Sbjct: 361 LSSLSEIGKDIVKMCGNVPLAIKVVGGSLCSRDTPEEWLYVRDAHRSKAQHTIGSDIMPI 420
Query: 421 LKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNNEDIGGQYFSELL 480
LKLSY LPPALKQCFAYCSLF K Y++ K LVHLWMAQGYFE S ++DIG +YF ELL
Sbjct: 421 LKLSYGDLPPALKQCFAYCSLFKKGYKFSKTELVHLWMAQGYFEAS-SKDIGDRYFLELL 480
Query: 481 GRNLFQDPEQDYR-GIETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGG 540
RNLFQDP++D I + MHDLVHDLA VAG E +V
Sbjct: 481 RRNLFQDPKEDLEANITSCIMHDLVHDLAQNVAGHE-----------------SIVAGES 540
Query: 541 ENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQALSLE 600
+ ++ + LI HV LG FKA L AR +RSL + + +
Sbjct: 541 SSSQVTDGLI----HVNLG-----------RFKAPPLLFEARMLRSLF-----SRNVECD 600
Query: 601 RVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTHIECLPDAITKLDNLQTLNI 660
R++ KSLRVL + R+P+SIG+LV+LRYLNL I+ LP AIT+L+NLQTLN+
Sbjct: 601 RLISNFKSLRVLSLKDAWLDRLPDSIGRLVYLRYLNLNDNFIKYLPQAITRLENLQTLNL 660
Query: 661 MGCRRLKELPRNFGKLTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMIGEGNGIDSLP 720
C RLKELPR+F KL NLR+L SGLT +P GFG +T LQEL F++ E GIDS+P
Sbjct: 661 QNCLRLKELPRDFTKLPNLRHLAIGGSGLTTMPPGFGTMTNLQELDVFVVEENFGIDSMP 720
Query: 721 ALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSF-NFEMEEA--ALGEMDKML 780
ALNL+GNL I+F K +++VLEA+ ANLK + +L+ L + E E+A + M++ML
Sbjct: 721 ALNLMGNLHIKFCKRRNDAVLEAQRANLKGSQRLSNLTLDYRELEREKATPSFSGMNEML 780
Query: 781 MCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNNCRVVPHMSRLLHLKYLR 840
M LQLPPNL L V Y G +M RWLD L KL SI I ECN C+ +PH+S+L HLK L
Sbjct: 781 MYLQLPPNLIYLRVGNYSGDKMQCRWLDVLSKLASIIISECNTCKTLPHLSQLPHLKNLY 840
Query: 841 LKFLDTLEYVED-DDIWVGNNV-----YFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLL 900
L+ LD LEYVED DDIWVG + YFP+LE L L L +LK WTRPSK GE R+ L
Sbjct: 841 LERLDALEYVEDEDDIWVGEDCGYEAHYFPALENLELDGLPQLKRWTRPSKSGGEQRRQL 900
Query: 901 FPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYKGVEMPR 960
FP L +EV C +L+S PLAP LESL+ + LFESNL D +P
Sbjct: 901 FPRLFQMEVCNCKELVSFPLAPKLESLKVSRGNAKLFESNLVLHDD-----------IPC 960
Query: 961 RWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVEDEDDIWVGDSASA 1020
+WL GL K+VSI+I+GC +C V+PHMS L LK ++LE LD LEYVEDEDDI +GD
Sbjct: 961 KWLVGLSKIVSISISGCNNCTVLPHMSHLPLLKKVYLERLDGLEYVEDEDDI-LGDGDCG 1020
Query: 1021 AHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQ----LFPRLLKMDVRACPKLMSMPL 1080
+ YFP+LE L L L ELK W+RPSK+ E+RQ LFP LL M+V+ C +L+ MPL
Sbjct: 1021 YTSHYFPALEKLNLISLPELKKWRRPSKNGGEKRQQRQLLFPHLLHMEVKHCKELVLMPL 1080
Query: 1081 APMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLEMRWIDDGLV 1140
AP LESL+ + N KLFESNLAS P + SSLFTSLKHL ID
Sbjct: 1081 APKLESLEVFRSNEKLFESNLAS-----------PNKEASSSLFTSLKHLR---IDGTKD 1140
Query: 1141 SLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYCNR-LENISG 1200
SL IS RLC LE++ + + +LRS+ + S PSSIR L + + N + NI
Sbjct: 1141 SLSISSRLCKLESVHITNGMELRSLMIES----------PSSIRDLQLAWVNSGICNILE 1200
Query: 1201 GNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEEKKEPGKIT 1260
+ +E L I+GS E E E N+ + +L++ NL KK P I
Sbjct: 1201 EDFPFIETLEIRGSSE----ELEEGNT--STYWQGLKSLRSLQLHYMNNL--KKLPPGIA 1260
Query: 1261 RSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEMGNLSL--- 1320
L +L+ L + + L +P IG L+ L+ L I + +L LPEE+GNLSL
Sbjct: 1261 -----CLTNLQDLYIGYMKNLIALPDEIGNLSLLQKLTIARVEKLIALPEEIGNLSLLSR 1282
Query: 1321 LKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYE-KPDGEDCHLIQHIPNVK 1355
L+I+ C +L +LP SL LTSL L I CP L +RY K +G D HL+QHIP+++
Sbjct: 1321 LEIFRCRELRILPLSLQGLTSLQELLIYGCPHLEERYSVKFNGRDRHLLQHIPDLQ 1282
BLAST of Spo25618.1 vs. NCBI nr
Match:
gi|731317080|ref|XP_010667657.1| (PREDICTED: disease resistance protein RGA2-like [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 995.3 bits (2572), Expect = 1.000e-286
Identity = 623/1379 (45.18%), Postives = 833/1379 (60.41%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MAEG++ +L +KALE L+ + ++ SSWWGAR DLKKL ++ MIQARVRDAE+ QE E
Sbjct: 1 MAEGVISDLVAKALELLATSTFKKASSWWGARDDLKKLGKSMEMIQARVRDAEKYQEEEG 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKVFSRSG-PIFNIKLAHKV 120
+++I LWL RLK VLY+A DLFD I+T+D + R +++ F RS P+ N K+A K+
Sbjct: 61 SDAIKLWLRRLKLVLYQAGDLFDRILTVDSQKQRRKQNHKVHFFLRSSSPVLNWKIAGKI 120
Query: 121 NSIREELNDIKSDMDVLNLKFFPAEELP-PLMMRLIKERQTSSFVKTEDIIGRENDTKLI 180
SIR++L+ I SDM LNL+ + + P PL+ R K R+T+SFV+ ED+IGRE D +I
Sbjct: 121 KSIRKQLDAINSDMAGLNLRVYDHRKEPQPLISRFRKNRETTSFVEAEDVIGRETDKDVI 180
Query: 181 IGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPE 240
I ML DPK + GE +++IPI+GFGGLGKTTLAQ V+ND+ VQ HFD+T W VPE
Sbjct: 181 IQMLLDPKYD------GEKVTIIPIMGFGGLGKTTLAQFVFNDQRVQNHFDLTKWAYVPE 240
Query: 241 VDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLDL 300
+D AVM +++ +FT + L +++++ IR+SI + YLLVLDDIWDE+ R DL
Sbjct: 241 KNDLTAVMGKIFNSFTDKEYHRDLSLEKLQLSIRESIESKNYLLVLDDIWDETIHRLHDL 300
Query: 301 LRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERN 360
+ LL+CG+ GSKVIVTTRS VA + T E Y+LG LT+++SWNLF++ + QE +
Sbjct: 301 VNLLKCGKPGSKVIVTTRSDVVARGLATIEEPYRLGTLTDDQSWNLFERLTLKPRQEGSS 360
Query: 361 -PDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAV 420
L+ IGK+IV CGNVPLAI+V G L S+ + +EW +R++Q +KA+ G +M +
Sbjct: 361 LGSLSEIGKDIVKMCGNVPLAIKVAGGFLCSRDTPEEWLYVRDAQRAKAQHTIGSDIMPI 420
Query: 421 LKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNNEDIGGQYFSELL 480
LKLSYD LPPALKQCFAYCSLF K Y + + LVHLWMAQGYFE S+ DIG +YF ELL
Sbjct: 421 LKLSYDDLPPALKQCFAYCSLFEKGYNFSQTELVHLWMAQGYFEASSR-DIGDRYFLELL 480
Query: 481 GRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGG 540
RNLFQ+PE+D I + KMHDLVHDLA VAG E M L G+ D +V
Sbjct: 481 RRNLFQNPEKDLEANITSCKMHDLVHDLAQHVAGHESMVL-GESYSSQVTDALV------ 540
Query: 541 ENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRM-RSLLFDMGLEQALSL 600
HV LG F+A A M RSLL +++ +
Sbjct: 541 --------------HVNLGR-----------FEAPPLSAEAAGMLRSLLLHTKIDK---I 600
Query: 601 ERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTHIECLPDAITKLDNLQTLN 660
+R + + KSLRVL Y I R+P+SIG+LVHLRYLNL++++IECLP AIT+L+NLQTLN
Sbjct: 601 DRWIFRFKSLRVLSLEYYWINRLPDSIGRLVHLRYLNLRNSNIECLPQAITRLENLQTLN 660
Query: 661 IMGCRRLKELPRNFGKLTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMIGEGNGIDSL 720
+ C LKELPR+F KL NLR+L + S LT +P FG +T LQEL F++GE +GIDS+
Sbjct: 661 LY-CSELKELPRDFTKLPNLRHLAIAGSSLTTMPPEFGTMTNLQELDLFVVGENSGIDSV 720
Query: 721 PALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSFN-FEMEEAALGE--MDKM 780
PALNL+GNL I+F +++VLEA+ ANLK + +L+ L + E EEA M++M
Sbjct: 721 PALNLMGNLHIKFCLQRNDAVLEAQRANLKGSQRLSNLNLDYRELEREEATPSSSGMNEM 780
Query: 781 LMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNNCRVVPHMSRLLHLKYL 840
LM LQLPPNL L V Y G +M RWLD + KL+SI I ECNNC+ +PH+S+L HLK L
Sbjct: 781 LMYLQLPPNLIYLRVGNYSGDKMQCRWLDVMSKLVSIIISECNNCKALPHLSQLPHLKNL 840
Query: 841 RLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLL 900
LK LD LEYVED+D + +D G FP L
Sbjct: 841 YLKRLDALEYVEDEDDIL-------------------------VGEDCGYAAHY-FPALE 900
Query: 901 NLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYK--GVEMPRRWL 960
NLE+ + P+L +E L K G +R
Sbjct: 901 NLELIKLPQL-------------------------------KEWTRLSKSGGKRQQKRQR 960
Query: 961 DGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVEDEDDIWVGDSASAAHN 1020
+L+ +N+ C +C ++PHMS+L LK ++LE LD LEYVEDEDDIW GD +
Sbjct: 961 LLFPRLLQLNVRDCNNCTLLPHMSQLPLLKKVYLERLDALEYVEDEDDIW-GDGDCGYTS 1020
Query: 1021 VYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQ----LFPRLLKMDVRACPKLMSMPLAPM 1080
YFP+L L L L +LK W RPSK EQRQ LFP+LL+M V+ C +L+ M LAP
Sbjct: 1021 HYFPALVHLELIKLPQLKKWTRPSKSGGEQRQQRQLLFPQLLQMKVKDCKELVLMLLAPK 1080
Query: 1081 LESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLEMRWIDDGLVSLR 1140
LESL NGKLFESNLAS P + SSLFTSLKHL ID SL
Sbjct: 1081 LESLDVTLGNGKLFESNLAS-----------PNKEASSSLFTSLKHLR---IDGTKNSLS 1140
Query: 1141 ISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYCNRLENISGGNLS 1200
IS RLC LE++E+R+C++LRS+ + SS+ R+ L+ +N +CN L+ G+
Sbjct: 1141 ISSRLCKLESVEIRYCEELRSLMIESSSSIRDLKLI-----EVNSGFCNILK----GDFP 1200
Query: 1201 VLEKLVIKGSCE-----NLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEEKKEPGKI 1260
+E L I GS E N ++ S+ L++ M KK P +I
Sbjct: 1201 FIETLKIWGSSEELEEGNTTTYWQGLRSLRSLELCYM----------------KKLPPRI 1234
Query: 1261 TRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEMGN---LS 1320
L +L+ L + ++ L +P IG L L+ L I + +L LP+E+GN LS
Sbjct: 1261 A-----CLTNLQTLYVCNVENLIALPDEIGNLFLLQKLTIARMEKLIALPKEIGNLSKLS 1234
Query: 1321 LLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYE-KPDGEDCHLIQHIPNVKFY 1357
L+I+DC +L +LP SL LTSL +L I CP L+ RY + +G DCHL+QHI ++K +
Sbjct: 1321 YLEIHDCRELRVLPLSLQGLTSLQTLIIVECPYLKVRYSVRFNGRDCHLLQHITDLKIW 1234
BLAST of Spo25618.1 vs. NCBI nr
Match:
gi|731317086|ref|XP_010667676.1| (PREDICTED: putative disease resistance protein RGA3 isoform X1 [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 921.0 bits (2379), Expect = 2.400e-264
Identity = 509/945 (53.86%), Postives = 650/945 (68.78%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MA+ ++ L +K L S+ + A EE +SW GA DLKKL+ T++MI+ RVRDAE+RQE +N
Sbjct: 1 MAQALMAALVAKVLISMGEFAYEETASWIGANDDLKKLKKTMKMIEYRVRDAEKRQEDDN 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKV---FSRSGPIFNIKLAH 120
+ES+ LWL RL+Q+LYRADDLFD I+TL R+ R EK++ KV S PI +KLA
Sbjct: 61 SESVKLWLYRLRQLLYRADDLFDNILTLKRQKKRDEKTTFKKVGILLSIPNPINTVKLAL 120
Query: 121 KVNSIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKL 180
K+ SIR+EL+ I SDM+ LNLK +EEL + R +K+R+T+SFV ED+IGR+ND +
Sbjct: 121 KIRSIRKELDAIISDMNGLNLKILVSEELELPINRFMKKRETASFVLKEDVIGRKNDINM 180
Query: 181 IIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVP 240
II MLFDP E + + ++VIPIVGFGG+GKTTLAQ V++DE+VQ HFDV W CVP
Sbjct: 181 IIDMLFDP------EYDSKRVTVIPIVGFGGIGKTTLAQFVFSDESVQNHFDVVQWACVP 240
Query: 241 EVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLD 300
VD+Q VM +V+++FT +K S L +D++K GIR+SI + KYLLVLDD+WDE RDRWLD
Sbjct: 241 YVDNQRMVMEKVFKSFTGEKSS-ELSLDDVKLGIRESIIDKKYLLVLDDMWDEDRDRWLD 300
Query: 301 LLRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEER 360
+L LL+ G GSKVIVTTRS VA ++GT Y+LGLLT+EESW LFK+ AF SG EER
Sbjct: 301 MLSLLKSGGFGSKVIVTTRSDEVANILGTNSKPYRLGLLTKEESWILFKRVAFRSGVEER 360
Query: 361 NPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAV 420
NP L +IGKEIV CGNVPLAIRV+GSLLYSK SE+EW ++ QL KAKL E +M V
Sbjct: 361 NPSLVQIGKEIVGRCGNVPLAIRVIGSLLYSKGSEEEWRLFKDDQLLKAKLIEEGNIMPV 420
Query: 421 LKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNNEDIGGQYFSELL 480
LKLSYDYL ALKQCF YCSLFPKDY + KA+LVHLWMAQGYFEPSN DIG QYFS LL
Sbjct: 421 LKLSYDYLSSALKQCFTYCSLFPKDYEFVKADLVHLWMAQGYFEPSNT-DIGAQYFSVLL 480
Query: 481 GRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGG 540
GRN FQD ++D RG + T KMHDLVHDLA VA ++YM+L
Sbjct: 481 GRNFFQDSQEDARGNVSTCKMHDLVHDLAQHVA---------------GDEYMLL--GHQ 540
Query: 541 ENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLL--FDMGLEQALS 600
+I++ + LI HV L DG + +LL A++MRSLL FD L +
Sbjct: 541 PSIQITDGLI----HVNL--------DGRE---DPASLLAAKKMRSLLGFFDRTLANSYE 600
Query: 601 LERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTHIECLPDAITKLDNLQTL 660
L + + KSLRVL I +P+SI KL+ LRYLNL H +CLPD I +LDNLQTL
Sbjct: 601 L---ISRFKSLRVLSLENRGIETLPSSIEKLILLRYLNLSGNHFKCLPDFIVRLDNLQTL 660
Query: 661 NIMGCRRLKELPRNFGKLTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMI--GEGNGI 720
N+ C+ LK+LPR+F KL NLR+L+ LTDLP GFG++T L EL +F + E + I
Sbjct: 661 NLDDCKSLKKLPRDFTKLPNLRHLVIDWDSLTDLPPGFGEMTSLHELHRFEVRKAENSSI 720
Query: 721 DSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSFNFEMEEAALG--EMD 780
SLPALNL G+L I+F +W +V+EA+ NL N LT L F E E+ L E+D
Sbjct: 721 CSLPALNLRGDLTIKFREWRVNAVVEAQRVNLTANGHLTSLSLKFQLEGEQVTLASCEID 780
Query: 781 KMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNNCRVVPHMSRLLHLK 840
ML CL LPP L+ +E Y G +MP RWLDGL LIS++I CN+CRVVPH+ L HLK
Sbjct: 781 DMLACLHLPPYLKYMEFDGYIGHKMPHRWLDGLTNLISVSISRCNSCRVVPHLGMLPHLK 840
Query: 841 YLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPC 900
+L +K L+ LEY+ED+ + +N Y P LE L L++L +LKGWTRPS D GE ++LLFP
Sbjct: 841 FLYIKDLEALEYIEDEAGILADNDYLPFLEVLELEDLPQLKGWTRPSNDTGEQKKLLFPR 900
Query: 901 LLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEE 936
L ++++ C +L+SMP AP LESL+ I+G + +S LT + D E
Sbjct: 901 LFQMKLNGCRELISMPQAPKLESLDVRRINGNMLKSILTLLHDHE 902
BLAST of Spo25618.1 vs. NCBI nr
Match:
gi|731317094|ref|XP_010667701.1| (PREDICTED: putative disease resistance protein RGA3 isoform X2 [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 921.0 bits (2379), Expect = 2.400e-264
Identity = 509/945 (53.86%), Postives = 650/945 (68.78%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MA+ ++ L +K L S+ + A EE +SW GA DLKKL+ T++MI+ RVRDAE+RQE +N
Sbjct: 1 MAQALMAALVAKVLISMGEFAYEETASWIGANDDLKKLKKTMKMIEYRVRDAEKRQEDDN 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKV---FSRSGPIFNIKLAH 120
+ES+ LWL RL+Q+LYRADDLFD I+TL R+ R EK++ KV S PI +KLA
Sbjct: 61 SESVKLWLYRLRQLLYRADDLFDNILTLKRQKKRDEKTTFKKVGILLSIPNPINTVKLAL 120
Query: 121 KVNSIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKL 180
K+ SIR+EL+ I SDM+ LNLK +EEL + R +K+R+T+SFV ED+IGR+ND +
Sbjct: 121 KIRSIRKELDAIISDMNGLNLKILVSEELELPINRFMKKRETASFVLKEDVIGRKNDINM 180
Query: 181 IIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVP 240
II MLFDP E + + ++VIPIVGFGG+GKTTLAQ V++DE+VQ HFDV W CVP
Sbjct: 181 IIDMLFDP------EYDSKRVTVIPIVGFGGIGKTTLAQFVFSDESVQNHFDVVQWACVP 240
Query: 241 EVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLD 300
VD+Q VM +V+++FT +K S L +D++K GIR+SI + KYLLVLDD+WDE RDRWLD
Sbjct: 241 YVDNQRMVMEKVFKSFTGEKSS-ELSLDDVKLGIRESIIDKKYLLVLDDMWDEDRDRWLD 300
Query: 301 LLRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEER 360
+L LL+ G GSKVIVTTRS VA ++GT Y+LGLLT+EESW LFK+ AF SG EER
Sbjct: 301 MLSLLKSGGFGSKVIVTTRSDEVANILGTNSKPYRLGLLTKEESWILFKRVAFRSGVEER 360
Query: 361 NPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAV 420
NP L +IGKEIV CGNVPLAIRV+GSLLYSK SE+EW ++ QL KAKL E +M V
Sbjct: 361 NPSLVQIGKEIVGRCGNVPLAIRVIGSLLYSKGSEEEWRLFKDDQLLKAKLIEEGNIMPV 420
Query: 421 LKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNNEDIGGQYFSELL 480
LKLSYDYL ALKQCF YCSLFPKDY + KA+LVHLWMAQGYFEPSN DIG QYFS LL
Sbjct: 421 LKLSYDYLSSALKQCFTYCSLFPKDYEFVKADLVHLWMAQGYFEPSNT-DIGAQYFSVLL 480
Query: 481 GRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGG 540
GRN FQD ++D RG + T KMHDLVHDLA VA ++YM+L
Sbjct: 481 GRNFFQDSQEDARGNVSTCKMHDLVHDLAQHVA---------------GDEYMLL--GHQ 540
Query: 541 ENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLL--FDMGLEQALS 600
+I++ + LI HV L DG + +LL A++MRSLL FD L +
Sbjct: 541 PSIQITDGLI----HVNL--------DGRE---DPASLLAAKKMRSLLGFFDRTLANSYE 600
Query: 601 LERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTHIECLPDAITKLDNLQTL 660
L + + KSLRVL I +P+SI KL+ LRYLNL H +CLPD I +LDNLQTL
Sbjct: 601 L---ISRFKSLRVLSLENRGIETLPSSIEKLILLRYLNLSGNHFKCLPDFIVRLDNLQTL 660
Query: 661 NIMGCRRLKELPRNFGKLTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMI--GEGNGI 720
N+ C+ LK+LPR+F KL NLR+L+ LTDLP GFG++T L EL +F + E + I
Sbjct: 661 NLDDCKSLKKLPRDFTKLPNLRHLVIDWDSLTDLPPGFGEMTSLHELHRFEVRKAENSSI 720
Query: 721 DSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSFNFEMEEAALG--EMD 780
SLPALNL G+L I+F +W +V+EA+ NL N LT L F E E+ L E+D
Sbjct: 721 CSLPALNLRGDLTIKFREWRVNAVVEAQRVNLTANGHLTSLSLKFQLEGEQVTLASCEID 780
Query: 781 KMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNNCRVVPHMSRLLHLK 840
ML CL LPP L+ +E Y G +MP RWLDGL LIS++I CN+CRVVPH+ L HLK
Sbjct: 781 DMLACLHLPPYLKYMEFDGYIGHKMPHRWLDGLTNLISVSISRCNSCRVVPHLGMLPHLK 840
Query: 841 YLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPC 900
+L +K L+ LEY+ED+ + +N Y P LE L L++L +LKGWTRPS D GE ++LLFP
Sbjct: 841 FLYIKDLEALEYIEDEAGILADNDYLPFLEVLELEDLPQLKGWTRPSNDTGEQKKLLFPR 900
Query: 901 LLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEE 936
L ++++ C +L+SMP AP LESL+ I+G + +S LT + D E
Sbjct: 901 LFQMKLNGCRELISMPQAPKLESLDVRRINGNMLKSILTLLHDHE 902
BLAST of Spo25618.1 vs. UniProtKB/TrEMBL
Match:
A0A0K9RNZ6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_050860 PE=4 SV=1)
HSP 1 Score: 1349.0 bits (3490), Expect = 0.000e+0
Identity = 666/670 (99.40%), Postives = 668/670 (99.70%), Query Frame = 1
Query: 695 LTCLQELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLK 754
L+ +ELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLK
Sbjct: 178 LSLERELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLK 237
Query: 755 FSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIREC 814
FSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIREC
Sbjct: 238 FSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIREC 297
Query: 815 NNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWT 874
NNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWT
Sbjct: 298 NNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWT 357
Query: 875 RPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDE 934
RPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDE
Sbjct: 358 RPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDE 417
Query: 935 EEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVE 994
EEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVE
Sbjct: 418 EEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVPHMSRLLHLKDLHLEELDTLEYVE 477
Query: 995 DEDDIWVGDSASAAHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQLFPRLLKMDVRA 1054
DEDDIWVGDSASAAHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQLFPRLLKMDVRA
Sbjct: 478 DEDDIWVGDSASAAHNVYFPSLEVLTLHYLRELKGWKRPSKDYAEQRQLFPRLLKMDVRA 537
Query: 1055 CPKLMSMPLAPMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLE 1114
CPKLMSMPLAPMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLE
Sbjct: 538 CPKLMSMPLAPMLESLKAYFINGKLFESNLASVEDEEEASSSLPQSSSPSSLFTSLKHLE 597
Query: 1115 MRWIDDGLVSLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYC 1174
MRWIDDGLVSLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYC
Sbjct: 598 MRWIDDGLVSLRISCRLCNLETLELRHCDQLRSITLGSSAPYRNSILLPSSIRHLNIYYC 657
Query: 1175 NRLENISGGNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEE 1234
NRLENISGGNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEE
Sbjct: 658 NRLENISGGNLSVLEKLVIKGSCENLYVEYESPNSIEDLDMGNMSGPETLKISCSKNLEE 717
Query: 1235 KKEPGKITRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEM 1294
KKEPGKITRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEM
Sbjct: 718 KKEPGKITRSMWQQLKSLRRLELSSITKLKKIPKGIGCLTTLETLKIWSLSELTQLPEEM 777
Query: 1295 GNLSLLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYEKPDGEDCHLIQHIPNVK 1354
GNLSLLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYEKPDGEDCHLIQHIPNVK
Sbjct: 778 GNLSLLKIYDCPKLVLLPQSLLALTSLHSLEIGNCPDLRKRYEKPDGEDCHLIQHIPNVK 837
Query: 1355 FYEKLIKYFD 1365
FYEKLIKYFD
Sbjct: 838 FYEKLIKYFD 847
BLAST of Spo25618.1 vs. UniProtKB/TrEMBL
Match:
A0A0D2NDD9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1)
HSP 1 Score: 566.2 bits (1458), Expect = 1.100e-157
Identity = 400/1131 (35.37%), Postives = 590/1131 (52.17%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MAE ++ +A L + AL+E WG + L+KL++T+ I+A + DAE Q A++
Sbjct: 1 MAEAGLFNIADGILGKIGNLALQEIGLVWGVKEQLEKLKNTVSTIKAVLLDAED-QHAKS 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIIT-LDRRNLRMEKSSMAKVFSRSGPI-FNIKLAHK 120
+E + WL +LK +Y DDL D T + +R ++ +FS+ + +N+K++H+
Sbjct: 61 HE-VRDWLGKLKDAVYDTDDLLDNFSTHVLKRQQGKRGKQVSFLFSKVSQVTYNLKISHQ 120
Query: 121 VNSIREELNDIKSDMDVLNLKFFPAEELP-PLMMRLIKERQTSSFVKTEDIIGRENDTKL 180
+ +IRE+L+ I +D +K+ + P + + ++ RQT SFV+ ED++GR+ D
Sbjct: 121 IEAIREKLDAIAAD----KIKYHFTDRSPISIPLVKVERRQTHSFVRKEDVVGRQGDKDA 180
Query: 181 IIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVP 240
I+ L E NG + +SVIPIVG GG GKTTLAQL YNDE KHF++ WVCV
Sbjct: 181 IMKRLL----ESNGV---DNVSVIPIVGIGGQGKTTLAQLAYNDERTVKHFELRMWVCVS 240
Query: 241 EVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLD 300
+V D ++ ++ ++ T K L +D ++ +R+ I+ KYLLVLDD+W++SR+RWL+
Sbjct: 241 DVFDVKMIVEKILESATSSKYES-LEMDSLQTHLRKRIDGRKYLLVLDDMWNDSRERWLN 300
Query: 301 LLRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEER 360
L LL G +GSK+IVTTR+ VA + GT + Y L L E+ SW+LF+K AF+ +E
Sbjct: 301 LADLLMNGARGSKIIVTTRAQLVASITGTSQ-PYLLEGLPEDMSWSLFEKVAFKESKETN 360
Query: 361 NPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAV 420
+ L IGK+IV C PL IR +G +LY+K +E EW ++ QLS E D +++V
Sbjct: 361 DSRLVAIGKDIVKKCAGNPLVIRTIGGVLYTKDTETEWLSLKERQLSMLTRNEDD-VLSV 420
Query: 421 LKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNN----EDIGGQYF 480
LKLSY+ LP LKQCFAYCSLFPKDY K L+ LW+A+G+ +P E++GGQYF
Sbjct: 421 LKLSYEQLPSYLKQCFAYCSLFPKDYEINKQMLISLWIAEGFIQPLQGIQCLEELGGQYF 480
Query: 481 SELLGRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLV 540
+LL R+ FQD E D G + + K+HDL+HDLA +AG + +V
Sbjct: 481 MDLLRRSFFQDVEYDEWGNVISCKLHDLMHDLAQLIAGSDCS----------------MV 540
Query: 541 GAGGENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQA 600
ENI HV L ++ D +K TTLLNA ++R+ L M
Sbjct: 541 DLDCENIS------ERTHHVSLS------AELDSSWKIPTTLLNANKIRTFLLPMQPIHR 600
Query: 601 LSLERV-----VLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTH-IECLPDAIT 660
+ L++V + + +RVLD + +P IGKL HLRYL+L I LP +IT
Sbjct: 601 VVLDKVDHEAIISSFRLMRVLDLHNTGLHILPRIIGKLKHLRYLDLSKNEVIRKLPSSIT 660
Query: 661 KLDNLQTLNIMGCRRLKELPRNFGKLTNLRYLINSK-SGLTDLPSGFGKLTCLQELSKFM 720
+L NLQTL I C+RL++LPR + +L++L + +GLT +PSG G+LT LQ L++F+
Sbjct: 661 ELLNLQTLKIYSCKRLEQLPRKLSNMISLKHLETGQCTGLTHMPSGIGQLTSLQTLTRFV 720
Query: 721 IG------EGNGIDSLPALN-LVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSFN 780
+G G+ L LN L G L I + E + ANLK L L ++
Sbjct: 721 VGMSSFEMASGGLRELKDLNELRGELMIAKLENLRNVAAECEEANLKEKQHLEVLTLDWS 780
Query: 781 FEMEEAALGEMDKMLM-CLQLPPNLEKLEVYLYKGVEMPRRWLDG-----LDKLISITIR 840
E+ E D+ L+ LQ NL++ +Y Y+ P +W+ L L+ ITI
Sbjct: 781 REVNNHVSFEEDEALLEGLQPHSNLQEFHIYGYRAERFP-KWMSFDMALLLPNLLEITIW 840
Query: 841 ECNNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDI-------WVGNNV--------YFP 900
C C +P S+L LK LRL+ + +EY+ED + GN + +FP
Sbjct: 841 NCIKCIHLPLFSQLPKLKVLRLEVITAVEYIEDSRAESSSSLSFKGNPMNRGREGKEFFP 900
Query: 901 SLEGLTLQNLRELKGWTRPSKD--------DGEPRQLL--------FPCLLNLEVSECPK 960
LE L +LR LKGW R + P++ L FPCL L++ C
Sbjct: 901 CLEELVFFDLRNLKGWWREAPPVTNNNHGAAASPQRPLQKKESMVSFPCLSKLKIGICTN 960
Query: 961 LMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYKGVEMPRRWLDGLDKLVSINI 1020
L MPL P LE LE N L + + VY ++ +DG+ LVS
Sbjct: 961 LTHMPLHPFLEELELKNTPARLLQQSAMVAAGANLVYPLYLSKLKVMHIDGIIDLVSFPE 1020
Query: 1021 TGCQHCRVVPHMS-----RLLHLKDLHLEELDTLEY-----VEDEDDIWVGDSASAAHNV 1063
G H + H+S L+ L + L+ L +L + E ++ G
Sbjct: 1021 KGIHHLTSLQHLSIENCPDLVCLTEEGLKSLTSLRFFNIRCCEILKSLFKGFK------- 1075
BLAST of Spo25618.1 vs. UniProtKB/TrEMBL
Match:
A0A061EA34_THECC (LRR and NB-ARC domains-containing disease resistance protein, putative OS=Theobroma cacao GN=TCM_011164 PE=3 SV=1)
HSP 1 Score: 554.3 bits (1427), Expect = 4.200e-154
Identity = 394/1137 (34.65%), Postives = 588/1137 (51.72%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MAE ++ +A L L L+E WG + +L+KL++T+ I+A + DAE Q A++
Sbjct: 8 MAEAFLFNIADGILGKLGNLTLQEMGLIWGVKEELEKLKNTVSTIKAVLLDAEE-QHAKS 67
Query: 61 NESINLWLSRLKQVLYRADDLFDEIIT--LDRRNLRMEK--SSMAKVFSRSGPI-FNIKL 120
+E I+ WL +LK +Y ADDL D+ T L R+ + K ++ +FS++ + +N+K+
Sbjct: 68 HEVID-WLGKLKDAVYDADDLLDDFSTHVLQRQVMMQGKRGKQVSFLFSKANQVAYNLKI 127
Query: 121 AHKVNSIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIKERQTSSFVKTEDIIGRENDT 180
H++ +IR+ L+ I +D + F L + + ++ +QT SFV+ E ++GRE D
Sbjct: 128 GHQIKAIRQRLDAIAADKTKYH---FTDRSLVSIPVVKVERKQTHSFVRKEGVVGREGDK 187
Query: 181 KLIIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVC 240
+ I+ L D N +SVIPIVG GG GKTT+A+LVYNDE + KHF++ WVC
Sbjct: 188 EAIMKRLLDSDVADN-------VSVIPIVGIGGQGKTTVAELVYNDEKIVKHFELRIWVC 247
Query: 241 VPEVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRW 300
V +V D + +++ ++ T K L +D ++ +R+ I+ KYLL+LDD+W+++R+RW
Sbjct: 248 VSDVFDVKLIAQKMLESATNAKFEN-LEMDSLQTHLRKRIDGRKYLLILDDVWNDNRERW 307
Query: 301 LDLLRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQE 360
+L LL G +GSK+IVTTR+ VA + GT E Y L L E+ SW+L +K AF+ G+E
Sbjct: 308 RNLRDLLMNGARGSKIIVTTRAQVVASITGTTEP-YLLEGLPEDMSWSLLEKMAFKEGRE 367
Query: 361 ERNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLM 420
N L IGK+IV C PLAIR +G +LY+K +E EW ++ QL + + D ++
Sbjct: 368 PNNSRLVAIGKDIVKRCAGNPLAIRTIGGVLYTKDTETEWLSLKEGQL--LMITQKDDVL 427
Query: 421 AVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNN----EDIGGQ 480
+LKLSY+ LP LKQCFAYCSLFP+DY K L+ LWMA+G+ + E++G Q
Sbjct: 428 PILKLSYEQLPSYLKQCFAYCSLFPRDYEINKQMLITLWMAEGFIQSLQGMQRLEELGDQ 487
Query: 481 YFSELLGRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMV 540
YF +LL R+ FQD E D G + + KMHDL+HDLA VAG +
Sbjct: 488 YFMDLLRRSFFQDVEYDEWGNVISCKMHDLMHDLAQLVAGSD----------------SS 547
Query: 541 LVGAGGENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLE 600
+V +NI RHV ++ D +K T LL A ++R+ L +
Sbjct: 548 MVDLDCKNIS------ERIRHVSFN------AELDSSWKIPTPLLKATKIRTFLLPVQPV 607
Query: 601 QALSL-----ERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTH-IECLPDA 660
+ L E V+ + LR+LD I +P+SIG L HLRYL+L I LP +
Sbjct: 608 HRVILDKVDHETVISSFRLLRLLDLHNTGIDILPSSIGTLKHLRYLDLSKNEVIRRLPSS 667
Query: 661 ITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYLINSK-SGLTDLPSGFGKLTCLQELSK 720
IT+L NLQTL + C+RL+ELPR +T+LR+L + +GLT +PSG G+LT LQ L++
Sbjct: 668 ITELLNLQTLKLCSCKRLEELPRKLRNMTSLRHLETGQCTGLTRMPSGLGQLTSLQTLTR 727
Query: 721 FMIG------EGNGIDSLPALN-LVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFS 780
F++G G+ L LN L G + I + V E K AN K L L
Sbjct: 728 FVVGMDSFKRPSGGLRELKDLNDLRGEVMIAKLENLKNVVSECKEANSKGKQHLEVLTLE 787
Query: 781 FNFEMEEAALGEMD-KMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDG----LDKLISITI 840
++ E+ + E D ++L LQ NL++ +Y Y+ P+ L L L+ IT+
Sbjct: 788 WSREVNDHTTFEEDEEILEGLQPHSNLQEFHIYGYRAGSFPKWMLSDMSLVLPNLLEITL 847
Query: 841 RECNNCRVVPHMSRLLHLKYLRLKFLDTLEYVED-----DDIWVGNN------------V 900
CN C +P S L L+ LRL+ + +EY+ED + G N
Sbjct: 848 WRCNRCLHLPLFSHLPKLRVLRLEVVTAVEYIEDSGAESSSLSFGGNRLKGGTEGKESTA 907
Query: 901 YFPSLEGLTLQNLRELKGWTR------------PSKDDGEPRQ-----LLFPCLLNLEVS 960
+FP L+ L L +LR LKGW R + P Q FPCL L +
Sbjct: 908 FFPCLKQLMLFDLRNLKGWWREVTAIANDNLGLAAASSQRPLQQKESMTSFPCLSKLTIG 967
Query: 961 ECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYKGV---------EMPRR 1020
C L MPL P+LE LE + L + ++ + E++ + ++
Sbjct: 968 ICTNLTYMPLHPLLEELELKCVSAKLLQQSVMIAAETEQIPMAAAAHLSYPLYLSKLKVM 1027
Query: 1021 WLDGLDKLVSINITGCQHCRVVPHMS--RLLHLKDLHLEELDTLEYVEDED-DIWVGDSA 1063
+D + LVS G H + H+S L L E L++L + D+ S
Sbjct: 1028 HIDSIMDLVSFPEKGLHHLTSLQHLSIANCPKLVCLPEEGLNSLRFFFISGCDMLKSLSK 1087
BLAST of Spo25618.1 vs. UniProtKB/TrEMBL
Match:
W9R509_9ROSA (Putative disease resistance protein RGA4 OS=Morus notabilis GN=L484_023797 PE=3 SV=1)
HSP 1 Score: 549.3 bits (1414), Expect = 1.300e-152
Identity = 351/963 (36.45%), Postives = 541/963 (56.18%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAAL-EEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAE 60
MAE I++ +A +E L L +E +G ++L+KL+ T+ I+A + DAE++Q AE
Sbjct: 1 MAEAILFNVAESIIEKLGSETLGKEMGLLFGVNTELQKLKRTVSAIKAVLLDAEQKQ-AE 60
Query: 61 NNESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAK----VFSRSGPI-FNIK 120
N++ + WL +L+ +Y ADDL DE T + M S AK FS S + F +K
Sbjct: 61 NHQ-VKDWLQKLQDAVYYADDLVDEFYTEALQRRVMIGSGKAKKVRIFFSSSNQLAFMLK 120
Query: 121 LAHKVNSIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIKERQTSSFVKTEDIIGREND 180
+ HK+ +RE LN+IK + D F+ E L + + R+T S+V+ E++IGR+ D
Sbjct: 121 IGHKIKEVRETLNEIKDNKD-----FYLMERLEDTRVSSMA-RETHSYVREEEVIGRDAD 180
Query: 181 TKLIIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWV 240
I+ +L D + + E +SVIPIVG GGLGKTTLAQ ++NDE +QKHF++ +WV
Sbjct: 181 KLAIMKLLLDLESK-------EDVSVIPIVGIGGLGKTTLAQHIFNDENIQKHFELRSWV 240
Query: 241 CVPEVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDR 300
CV + D ++ ++ ++ T K SG L +++++ +R+ I+ +YLLVLDD+W+E ++
Sbjct: 241 CVSDFFDLKLIVEKIIKSVTL-KSSGNLELEQLQFHLRKEIDGKRYLLVLDDVWNEDGEK 300
Query: 301 WLDLLRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQ 360
W L RLL G KGS+V+VTTRS VA + T + YKL L +SW+LFK+ AF+ GQ
Sbjct: 301 WRRLERLLMGGAKGSRVLVTTRSYMVAKITHTIQP-YKLSGLDNVDSWSLFKRIAFDKGQ 360
Query: 361 EERNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRL 420
E N + +G EIV+ C VPLAI+ +GS+LY K E EW ++LSK E D +
Sbjct: 361 EPENSTVLAMGMEIVEKCRGVPLAIKTIGSILYFKRREIEWSSFMETELSKIPQGEDD-I 420
Query: 421 MAVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNN----EDIGG 480
+ LKLSY++LP LK CFAYC LFPKD+ + L+ LW+AQG+ +PS+ ED+G
Sbjct: 421 LPALKLSYNHLPSHLKHCFAYCRLFPKDHEIDVQKLIRLWIAQGFIKPSDRSQCLEDVGY 480
Query: 481 QYFSELLGRNLFQDPE-QDYRGIETYKMHDLVHDLALFVAGGEIMEL-QGQDAEDDDEDY 540
+YF LL R+ FQDP+ D+ ++ +++HDL+HDLA+ VAG + + + + +++ +
Sbjct: 481 EYFMALLWRSFFQDPKIDDWGNVKRFRIHDLMHDLAVLVAGTSSISIDRSMENSNENIRH 540
Query: 541 MVLVGAGGENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMG 600
+ ++ +L L+++ VR ++ + + T + +++R L D
Sbjct: 541 VSFDSKFNQSWKLPPSLLKKNNIVRTFISPKYNAWHGYQHNFLGT--SRKKLRWLTRD-- 600
Query: 601 LEQALSLERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTHIECLPDAITKL 660
++ SLR LD + I ++PNS+G+L HLRYL++ +I+ LP++IT+L
Sbjct: 601 --------AILFSFTSLRTLDLKALNIKKLPNSLGRLKHLRYLDVSRNNIQMLPNSITRL 660
Query: 661 DNLQTLNIMGCRRLKELPRNFGKLTNLRYL-INSKSGLTDLPSGFGKLTCLQELSKFMIG 720
NLQTL + CR L LP + KL NLR+L + L+ +P G G+LT L L +F++
Sbjct: 661 QNLQTLILSHCRELIRLPEDMKKLVNLRHLQTHGCRRLSYMPRGIGELTALHSLDRFVVA 720
Query: 721 E-GN------GIDSLPALNLVG-NLDIEFNKWGSESVLEAKGANLKNNHQLTCLK--FSF 780
+ GN G+ L LN +G L IE G LE K AN K+ L L + +
Sbjct: 721 DTGNVTRRTSGLSELGGLNNLGAELSIENLGHGKNIALEVKAANFKDKQHLQSLVLFWRY 780
Query: 781 NFEMEEAALGEMDKM-LMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNN 840
F+ ++ ++ E D+M L L+ ++ L+V + G+ WL L+ L+ + + C
Sbjct: 781 GFDGDQTSVVENDEMSLDDLRPHSKMKALKVVNFMGLRF-AGWLSTLNNLVKLELLSCEK 840
Query: 841 CRVVPHMSRLLHLKYLRLKFLDTLEYVED--------DDIWVGNNVYFPSLEGLTLQNLR 900
C+ +PH+ L LK L L L LEY+ D + +FPSL L ++
Sbjct: 841 CQHLPHLHELPLLKELTLYALIALEYISDMESNNQLSTSLSTPTTPFFPSLTILKIRFCL 900
Query: 901 ELKGWTRPSKDDGE----------------PRQLLFPCLLNLEVSECPKLMSMPLAPMLE 916
LKGW R S G+ Q +FPCL +LE+ CP L SMPL P L+
Sbjct: 901 NLKGWWRRSTIAGDNSGALGLTTATTPPLHQNQPVFPCLHDLEIRNCPHLTSMPLFPDLK 932
BLAST of Spo25618.1 vs. UniProtKB/TrEMBL
Match:
V4WEM4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10007328mg PE=3 SV=1)
HSP 1 Score: 547.0 bits (1408), Expect = 6.600e-152
Identity = 365/942 (38.75%), Postives = 520/942 (55.20%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MAEG++++ K LE L+ E +G + D+ L DT+ IQA + DAE + ++
Sbjct: 1 MAEGLLFDTVGKILEVLASQIFHEIGLAYGVQDDISNLRDTVDTIQAVLLDAEDKHSRKD 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAK----VFSRSGPI-FNIKL 120
+ ++ +WL RLK +Y A+DL D+ T R M S + K FS+ + +++
Sbjct: 61 H-AVTIWLRRLKDAVYAAEDLLDDFSTEFLRRKLMSGSRVTKEVLLFFSKYNQFAYALEM 120
Query: 121 AHKVNSIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIK-ERQTSSFVKTEDIIGREND 180
K+ +IRE L IK+D L+ P R+ R+T SFV EDIIGR+ D
Sbjct: 121 GRKIKAIRERLESIKNDRQFHLLQ-------QPYERRVENTRRETHSFVHKEDIIGRDGD 180
Query: 181 TKLIIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWV 240
II L D + E E E ++VIPIVG GGLGKT +AQLVYNDE V+ HF++ WV
Sbjct: 181 KNEIIDRLLD-----SSESEIESVAVIPIVGIGGLGKTAVAQLVYNDEDVKTHFNLRMWV 240
Query: 241 CVPEVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDR 300
CV +V ++ ++ ++ T +++S +L +D+++ +R I+ +YLLVLDD+W+E+RD+
Sbjct: 241 CVSDVFGVTTIVEKMIRSAT-NRESEKLDLDQLQERLRGEIDGKRYLLVLDDVWNENRDK 300
Query: 301 WLDLLRLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQ 360
WL+L LL G GSK++VTTRS VA + +K + L L E+ SW+LF + AFE G
Sbjct: 301 WLELEALLMNGVSGSKIVVTTRSERVARIT-SKLPFHALRGLPEDMSWSLFTRMAFEQGS 360
Query: 361 EERNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRL 420
E ++ L +IGK++V C VPLAIR +G LLY +E W R+ +LSK +E D +
Sbjct: 361 EPKDSKLVQIGKDVVGKCAGVPLAIRTIGRLLYYNNTETYWLHFRDDELSKVPQEESD-I 420
Query: 421 MAVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNN----EDIGG 480
+ LKLSYD+LP LKQCFAYC+LFPKDY K LV LWMAQG+ S + ED+G
Sbjct: 421 LPKLKLSYDHLPSPLKQCFAYCALFPKDYLIVKEQLVLLWMAQGFLGLSIDNQCPEDVGH 480
Query: 481 QYFSELLGRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYM 540
+YF LL R+ FQD E D G I K+HDL+HDLA VAG E +++ DA + +E
Sbjct: 481 EYFMSLLSRSFFQDAEYDEWGNIIRCKIHDLMHDLAESVAGTECAKVK-LDARNVNE--- 540
Query: 541 VLVGAGGENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLL----- 600
H+ +S D + T LL A+ +R+ L
Sbjct: 541 ------------------RTHHI------SCVSGFDSSLEFPTALLRAKNLRTFLSTVYS 600
Query: 601 -FDMGLEQALSLERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQH-THIECLP 660
D L ++ ++V K LR L+ S EI +P+ IGKL HLRY NL H I+ LP
Sbjct: 601 SSDRQLNESY-CNKIVSSFKCLRTLNLSNSEIETVPSLIGKLKHLRYFNLSHNADIKSLP 660
Query: 661 DAITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYL-INSKSGLTDLPSGFGKLTCLQEL 720
D++++L NLQTL++ C L ELPR+ GK+ +LR+L I S LTD+P+G G+LT L+ L
Sbjct: 661 DSVSRLLNLQTLDLSCCDDLVELPRDIGKMVSLRHLAIESCLSLTDMPNGLGQLTNLRTL 720
Query: 721 SKFMIGEGNGIDSLPALN-LVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSFNFE 780
FM+G + L LN L G+L IE G + ++ ANL+ L L ++
Sbjct: 721 PLFMVGRKTQLSQLNGLNKLRGSLRIE--NLGEKQ--NSRLANLEAKEGLQSL--VLQWD 780
Query: 781 MEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNNCRVV 840
+ + D +L L+ NL++L + + G+ + WL + L I I C C+ +
Sbjct: 781 ANKTVIYIDDALLEGLKPHQNLKELTIIRFGGIRL-SSWLSSVTNLTMIDISICIKCQYI 840
Query: 841 PHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPSKDD 900
P + +L LK LRL L LEY+ + FPSLE L + ELKGW R D
Sbjct: 841 PELDQLPSLKRLRLFKLSALEYISSSS--PPSTTIFPSLEELRIFACPELKGWWR---TD 885
Query: 901 GEPRQL----LFPCLLNLEVSECPKLMSMPLAPMLESLEACN 919
G Q LFP L L + CPKL+ MPL P LE L C+
Sbjct: 901 GSTTQTAEPPLFPSLSKLTIDGCPKLVFMPLYPSLEELSLCS 885
BLAST of Spo25618.1 vs. ExPASy Swiss-Prot
Match:
RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)
HSP 1 Score: 388.7 bits (997), Expect = 2.700e-106
Identity = 297/985 (30.15%), Postives = 483/985 (49.04%), Query Frame = 1
Query: 29 WGARSDLKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQVLYRADDLFDEIITL 88
+G + KKL MIQA + DA+ +Q ++I WL +L Y DD+ D+ T
Sbjct: 25 FGFEKEFKKLSSMFSMIQAVLEDAQEKQL--KYKAIKNWLQKLNVAAYEVDDILDDCKT- 84
Query: 89 DRRNLRMEKSSMAKVFSRSGPIFNIKLAHKVNSIREELNDIKSDMDVLNLKFFPAEELPP 148
R +++ + + R+ F K+ ++ + E+L+ I + +L
Sbjct: 85 --EAARFKQAVLGRYHPRT-ITFCYKVGKRMKEMMEKLDAIAEERRNFHLD-------ER 144
Query: 149 LMMRLIKERQTSSFVKTEDIIGRENDTKLIIGMLFDPKCEYNGEGEGEGISVIPIVGFGG 208
++ R RQT + + GRE + I+ +L N E + V+PI+G GG
Sbjct: 145 IIERQAARRQTGFVLTEPKVYGREKEEDEIVKILI------NNVSYSEEVPVLPILGMGG 204
Query: 209 LGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRVYQAFTRDKDSGRLLVDEIK 268
LGKTTLAQ+V+ND+ + +HF++ WVCV + D+ +++ + ++ K G + + ++
Sbjct: 205 LGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESI-EGKSLGDMDLAPLQ 264
Query: 269 RGIRQSINNNKYLLVLDDIWDESRDRWLDLLRLLECGRKGSKVIVTTRSLTVAGVVGTKE 328
+ +++ +N +Y LVLDD+W+E +++W +L +L+ G G+ +++TTR + ++GT +
Sbjct: 265 KKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQ 324
Query: 329 ATYKLGLLTEEESWNLFKKFAFESGQEERNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYS 388
Y+L L++E+ W LFK+ AF Q E +P L IGKEIV CG VPLA + +G LL
Sbjct: 325 -LYQLSNLSQEDCWLLFKQRAF-CHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRF 384
Query: 389 KYSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKA 448
K E EWE +R+S++ E L A L+LSY +LP L+QCFAYC++FPKD + EK
Sbjct: 385 KREESEWEHVRDSEIWNLPQDENSVLPA-LRLSYHHLPLDLRQCFAYCAVFPKDTKIEKE 444
Query: 449 NLVHLWMAQGYFEPSNN---EDIGGQYFSELLGRNLFQDPEQDYRGIETYKMHDLVHDLA 508
L+ LWMA + N ED+G + ++EL R+ FQ+ E G +KMHDL+HDLA
Sbjct: 445 YLIALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQEIEVK-SGKTYFKMHDLIHDLA 504
Query: 509 --LFVAGGEIMELQGQDAEDDDEDYMVLVGAGGENIELNEPLIREFRHVRLGVTKGALSD 568
+F A ++ Q DDED M +V + + +G ++ S
Sbjct: 505 TSMFSASASSRSIR-QINVKDDEDMMFIVTNYKDMMS-------------IGFSEVVSSY 564
Query: 569 GDDVFKAVTTLLNARRMRSLLFDMGLEQALSLERVVLKCKSLRVLDFSYMEITRIPNSIG 628
+FK +L + ++ + L V LR LD S +I +P
Sbjct: 565 SPSLFKRFVSL--------RVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPK--- 624
Query: 629 KLVHLRYLNLQHTHIECLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYLINSKS 688
+ KL NLQTL++ C+ L LP+ KL +LR L+
Sbjct: 625 --------------------RLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC 684
Query: 689 GLTDLPSGFGKLTCLQELSKFMIGEGNG--IDSLPALNLVGNLDIEFNKWGSESVLEAKG 748
LT +P G LTCL+ L F++GE G + L LNL G + I + ++ +EAK
Sbjct: 685 PLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNLNLRGAISITHLE-RVKNDMEAKE 744
Query: 749 ANLKNNHQLTCLKFSFN----FEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPR 808
ANL L L S++ +E EE K+L L+ PNL+ LE+ + G +P
Sbjct: 745 ANLSAKANLHSLSMSWDRPNRYESEEV------KVLEALKPHPNLKYLEIIDFCGFCLP- 804
Query: 809 RWLDG--LDKLISITIRECNNCRVVPHMSRLLHLKYLRLKFLDT-LEYVEDDDIWVGNNV 868
W++ L ++SI I C NC +P L L+ L L+ +EYVED
Sbjct: 805 DWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRR- 864
Query: 869 YFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPM--LE 928
FPSL L + LKG R + FP L +++S+CP + L+ + LE
Sbjct: 865 -FPSLRKLHIGGFCNLKGLQRMKGAE------QFPVLEEMKISDCPMFVFPTLSSVKKLE 924
Query: 929 SLEACNIHGTLFESNLTSVVDEEEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVP- 988
+ G SNL+++ + + + L+ L+ ++++ ++ + +P
Sbjct: 925 IWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKELPT 924
Query: 989 HMSRLLHLKDLHLEELDTLEYVEDE 997
++ L +LK L + LE + +E
Sbjct: 985 SLASLNNLKCLDIRYCYALESLPEE 924
BLAST of Spo25618.1 vs. ExPASy Swiss-Prot
Match:
RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)
HSP 1 Score: 383.3 bits (983), Expect = 1.100e-104
Identity = 294/1010 (29.11%), Postives = 479/1010 (47.43%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
MAE + L L++L+ E +G + + ++L IQA + DA+ +Q N
Sbjct: 1 MAEAFIQVL----LDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQL--N 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKVFSRSGPIFNIKLAHKVN 120
N+ + WL +L Y DD+ DE T + R +S + + P F K+ +++
Sbjct: 61 NKPLENWLQKLNAATYEVDDILDEYKT---KATRFSQSEYGRYHPKVIP-FRHKVGKRMD 120
Query: 121 SIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKLIIG 180
+ ++L I + +L ++ R R+T S + + GR+ + I+
Sbjct: 121 QVMKKLKAIAEERKNFHLH-------EKIVERQAVRRETGSVLTEPQVYGRDKEKDEIVK 180
Query: 181 MLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVD 240
+L N + + +SV+PI+G GGLGKTTLAQ+V+ND+ V +HF W+CV E
Sbjct: 181 ILI------NNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDF 240
Query: 241 DQDAVMRRVYQAFTRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLLR 300
D+ +++ + ++ G + + +++ +++ +N +YLLVLDD+W+E + +W +L
Sbjct: 241 DEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRA 300
Query: 301 LLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERNPD 360
+L+ G G+ V+ TTR V ++GT + Y+L L++E+ W LF + AF QEE NP+
Sbjct: 301 VLKVGASGASVLTTTRLEKVGSIMGTLQ-PYELSNLSQEDCWLLFMQRAF-GHQEEINPN 360
Query: 361 LTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAVLKL 420
L IGKEIV G VPLA + +G +L K E+ WE +R+S + E ++ L+L
Sbjct: 361 LVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESS-ILPALRL 420
Query: 421 SYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNN---EDIGGQYFSELL 480
SY LP LKQCFAYC++FPKD + EK L+ LWMA G+ N ED+G + + EL
Sbjct: 421 SYHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELY 480
Query: 481 GRNLFQDPEQDYRGIETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGGE 540
R+ FQ+ E G +KMHDL+HDLA + ++ +M+ +G
Sbjct: 481 LRSFFQEIEVK-DGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEV 540
Query: 541 NIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQALSLER 600
P + +F +R+ L+ GD F ++ S + D+
Sbjct: 541 VFFYTLPPLEKFISLRV------LNLGDSTF---------NKLPSSIGDL---------- 600
Query: 601 VVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQH-THIECLPDAITKLDNLQTLNI 660
LR L+ + +P + KL +L+ L+LQ+ T + CLP +KL +L+ L
Sbjct: 601 -----VHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNL-- 660
Query: 661 MGCRRLKELPRNFGKLTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMIGEGNG--IDS 720
L++ LT +P G LTCL+ L +F++G G +
Sbjct: 661 ---------------------LLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGE 720
Query: 721 LPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSFNFEMEEAALGEMDKMLM 780
L LNL G++ I + ++ +AK ANL L L S+N E K+L
Sbjct: 721 LGNLNLYGSIKISHLE-RVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHIYESEEVKVLE 780
Query: 781 CLQLPPNLEKLEVYLYKGVEMPRRWLDG--LDKLISITIRECNNCRVVPHMSRLLHLKYL 840
L+ NL L++Y ++G+ +P W++ L ++SI I NC +P L L+ L
Sbjct: 781 ALKPHSNLTSLKIYGFRGIHLP-EWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESL 840
Query: 841 RLKFLDT-LEYVEDDDIWVGN----NVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLL 900
L + +EYVE+ DI V + + FPSL L + + LKG K +GE +
Sbjct: 841 ELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLKGLL---KKEGEEQ--- 900
Query: 901 FPCLLNLEVSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYKGVEMPR 960
FP L + + ECP L SNL ++ Y P
Sbjct: 901 FPVLEEMIIHECPFL--------------------TLSSNLRALTSLRICYNKVATSFPE 902
Query: 961 RWLDGLDKLVSINITGCQHCRVVP-HMSRLLHLKDLHLEELDTLEYVEDE 997
L L + I+ C + + +P ++ L LK L ++ LE + +E
Sbjct: 961 EMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEE 902
BLAST of Spo25618.1 vs. ExPASy Swiss-Prot
Match:
RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)
HSP 1 Score: 382.1 bits (980), Expect = 2.500e-104
Identity = 303/992 (30.54%), Postives = 478/992 (48.19%), Query Frame = 1
Query: 29 WGARSDLKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQVLYRADDLFDEIITL 88
+G + +KL IQA ++DA+ +Q + ++I WL +L Y DD+ E
Sbjct: 25 FGFEKECEKLSSVFSTIQAVLQDAQEKQLKD--KAIENWLQKLNSAAYEVDDILGEC--- 84
Query: 89 DRRNLRMEKSSMAKVFSRSGPI-FNIKLAHKVNSIREELNDIKSDMDVLNLKFFPAEELP 148
+R E+S + F G I F K+ ++ I E+L+ I + KF E+
Sbjct: 85 KNEAIRFEQSRLG--FYHPGIINFRHKIGRRMKEIMEKLDAISEE----RRKFHFLEK-- 144
Query: 149 PLMMRLIKERQTSS------FVKTED-IIGRENDTKLIIGMLFDPKCEYNGEGEGEGISV 208
I ERQ ++ FV TE + GR+ + I+ +L N E + V
Sbjct: 145 ------ITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILI------NNVNVAEELPV 204
Query: 209 IPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRVYQAFTRDKDSG 268
PI+G GGLGKTTLAQ+++NDE V KHF+ WVCV + D+ +++ + R
Sbjct: 205 FPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHV 264
Query: 269 RLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLLRLLECGRKGSKVIVTTRSLTV 328
L ++ +++ +N +YLLVLDD+W++ ++W L +L G +G+ ++ TTR V
Sbjct: 265 EDLA-SFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRLEKV 324
Query: 329 AGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERNPDLTRIGKEIVDSCGNVPLAIR 388
++GT + Y L L+ +S LF + AF Q+E NP+L IGKEIV CG VPLA +
Sbjct: 325 GSIMGTLQ-PYHLSNLSPHDSLLLFMQRAF-GQQKEANPNLVAIGKEIVKKCGGVPLAAK 384
Query: 389 VVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSYDYLPPALKQCFAYCSLFP 448
+G LL K E EWE +R++++ E ++ L+LSY +LP L+QCFAYC++FP
Sbjct: 385 TLGGLLRFKREESEWEHVRDNEIWSLPQDESS-ILPALRLSYHHLPLDLRQCFAYCAVFP 444
Query: 449 KDYRYEKANLVHLWMAQGYFEPSNN---EDIGGQYFSELLGRNLFQDPEQDYRGIETYKM 508
KD + K NL+ LWMA G+ N ED+G + ++EL R+ FQ+ E G +K+
Sbjct: 445 KDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEAK-SGNTYFKI 504
Query: 509 HDLVHDLA--LFVAG---GEIMELQGQDAEDDDEDYMVLVGAGGENIELNEPLIREFRHV 568
HDL+HDLA LF A G I E+ +D + + V +G + L+++F V
Sbjct: 505 HDLIHDLATSLFSASASCGNIREINVKDYK-----HTVSIGFAAVVSSYSPSLLKKF--V 564
Query: 569 RLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQALSLERVVLKCKSLRVLDFSY 628
L V + S + + ++ LL+ LR LD S
Sbjct: 565 SLRVLNLSYSKLEQLPSSIGDLLH----------------------------LRYLDLSC 624
Query: 629 MEITRIPNSIGKLVHLRYLNLQHTH-IECLPDAITKLDNLQTLNIMGCRRLKELPRNFGK 688
+P + KL +L+ L++ + + + CLP +KL +L+ L + GC
Sbjct: 625 NNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC------------ 684
Query: 689 LTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMIGEGNG--IDSLPALNLVGNLDI-EF 748
LT P G LTCL+ L F++G G + L LNL G++ I
Sbjct: 685 ------------PLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNLNLCGSISITHL 744
Query: 749 NKWGSESVLEAKGANLKNNHQLTCLKFSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVY 808
+ +++ E ANL L L S++ + + K+L L+ PNL+ LE+
Sbjct: 745 ERVKNDTDAE---ANLSAKANLQSLSMSWDNDGPNRYESKEVKVLEALKPHPNLKYLEII 804
Query: 809 LYKGVEMPRRWLDG--LDKLISITIRECNNCRVVPHMSRLLHLKYLRLKFLDT-LEYVED 868
+ G P W++ L+K+IS+ I+ C NC +P L L+ L L+ +EYVE+
Sbjct: 805 AFGGFRFP-SWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEE 864
Query: 869 DDIW--VGNNVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLLNLEVSECPKLM 928
DD+ FPSL+ L + R LKG K++GE + FP L + + CP L
Sbjct: 865 DDVHSRFSTRRSFPSLKKLRIWFFRSLKGL---MKEEGEEK---FPMLEEMAILYCP-LF 916
Query: 929 SMPLAPMLESLEA---CNIHGTLFESNLTSVVDEEEVYLYKGVEMPRRWLDGLDKLVSIN 988
P ++ LE N G SNL+++ Y+ +P L L ++
Sbjct: 925 VFPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLS 916
Query: 989 ITGCQHCRVVP-HMSRLLHLKDLHLEELDTLE 992
++ + +P ++ L LK L +E D+LE
Sbjct: 985 FFDFKNLKDLPTSLTSLNALKRLQIESCDSLE 916
BLAST of Spo25618.1 vs. ExPASy Swiss-Prot
Match:
RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)
HSP 1 Score: 362.8 bits (930), Expect = 1.600e-98
Identity = 283/986 (28.70%), Postives = 481/986 (48.78%), Query Frame = 1
Query: 29 WGARSDLKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQVLYRADDLFDEIITL 88
+G + + ++L IQA + DA+ +Q N++ + WL +L Y DD+ DE T
Sbjct: 25 FGFQDEFQRLSSMFSTIQAVLEDAQEKQL--NDKPLENWLQKLNAATYEVDDILDEYKTK 84
Query: 89 DRRNLRMEKSSMAKVFSRSGPI---FNIKLAHKVNSIREELNDIKSDMDVLNLKFFPAEE 148
R L+ E + R P F K+ +++ + ++LN I + +L+
Sbjct: 85 ATRFLQSE-------YGRYHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQ------ 144
Query: 149 LPPLMMRLIKERQTSSFVKTEDIIGRENDTKLIIGMLFDPKCEYNGEGEGEGISVIPIVG 208
++ R R+T S + + GR+ + I+ +L N + + +SV+PI+G
Sbjct: 145 -EKIIERQAATRETGSVLTEPQVYGRDKEKDEIVKILI------NTASDAQKLSVLPILG 204
Query: 209 FGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRVYQAFTRDKDSGRLLVD 268
GGLGKTTL+Q+V+ND+ V + F W+C+ + ++ +++ + ++ K + +
Sbjct: 205 MGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESI-EGKSLSDMDLA 264
Query: 269 EIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLLRLLECGRKGSKVIVTTRSLTVAGVVG 328
+++ +++ +N +Y LVLDD+W+E + +W +L +L+ G G+ V+ TTR V ++G
Sbjct: 265 PLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVGSIMG 324
Query: 329 TKEATYKLGLLTEEESWNLFKKFAFESGQEERNPDLTRIGKEIVDSCGNVPLAIRVVGSL 388
T + Y+L L+ E+ W LF + AF QEE NP+L IGKEIV CG VPLA + +G +
Sbjct: 325 TLQP-YELSNLSPEDCWFLFMQRAF-GHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGI 384
Query: 389 LYSKYSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSYDYLPPALKQCFAYCSLFPKDYRY 448
L K E+EWE +R+S + E ++ L+LSY +LP L+QCF YC++FPKD +
Sbjct: 385 LRFKREEREWEHVRDSPIWNLPQDESS-ILPALRLSYHHLPLDLRQCFVYCAVFPKDTKM 444
Query: 449 EKANLVHLWMAQGYFEPSNN---EDIGGQYFSELLGRNLFQDPEQDYRGIETYKMHDLVH 508
K NL+ WMA G+ N ED+G + ++EL R+ FQ+ E + G +KMHDL+H
Sbjct: 445 AKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVE-SGKTYFKMHDLIH 504
Query: 509 DLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGGENIELNEPLIREFRHVRLGVTKGALS 568
DLA + ++ + + YM+ +G + L+++F V L V S
Sbjct: 505 DLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYSPSLLQKF--VSLRVLNLRNS 564
Query: 569 DGDDVFKAVTTLLNARRMRSLLFDMGLEQALSLERVVLKCKSLRVLDFSYMEITRIPNSI 628
+ + + ++ L++ R L G + +L + + K ++L+ LD Y +
Sbjct: 565 NLNQLPSSIGDLVHLR----YLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCD-------- 624
Query: 629 GKLVHLRYLNLQHTHIECLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYLINSK 688
+ CLP +KL +L+ L + GC
Sbjct: 625 --------------SLSCLPKQTSKLGSLRNLLLDGCS---------------------- 684
Query: 689 SGLTDLPSGFGKLTCLQELSKFMIG--EGNGIDSLPALNLVGNLDI-EFNKWGSESVLEA 748
LT P G LTCL+ LS F+IG +G+ + L LNL G++ I + ++ ++ +A
Sbjct: 685 --LTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNLNLYGSISITKLDRVKKDT--DA 744
Query: 749 KGANLKNNHQLTCLKFSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRW 808
K ANL L L S++ + + E +L L+ NL+ LE+ + G+ +P W
Sbjct: 745 KEANLSAKANLHSLCLSWDLDGKHRYDSE---VLEALKPHSNLKYLEINGFGGIRLP-DW 804
Query: 809 LDG--LDKLISITIRECNNCRVVPHMSRLLHLKYLRLKFLDT-LEYVEDDDIWVGNNVY- 868
++ L ++SI IR C NC +P L L+ L L +EYVED NV+
Sbjct: 805 MNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELHTGSADVEYVED-------NVHP 864
Query: 869 --FPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLLNLEVSECPKLMSMPLAPMLES 928
FPSL L + + LKG K +GE + FP L + CP + +P +++
Sbjct: 865 GRFPSLRKLVIWDFSNLKGLL---KMEGEKQ---FPVLEEMTFYWCP-MFVIPTLSSVKT 911
Query: 929 LEACNIHGTLFE--SNLTSVVDEEEVYLYKGVEMPRRWLDGLDKLVSINITGCQHCRVVP 988
L+ T+ SNL ++ + + +P L L + I+ ++ + +P
Sbjct: 925 LKVIVTDATVLRSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNLKELP 911
Query: 989 -HMSRLLHLKDLHLEELDTLEYVEDE 997
++ L LK L E D LE + +E
Sbjct: 985 TSLASLNALKSLKFEFCDALESLPEE 911
BLAST of Spo25618.1 vs. ExPASy Swiss-Prot
Match:
R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 339.3 bits (869), Expect = 1.900e-91
Identity = 287/949 (30.24%), Postives = 461/949 (48.58%), Query Frame = 1
Query: 35 LKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQVLYRADDLFDEIITLDRR-NL 94
L++L + I A + DAE +Q N + W++ L+ V+Y A+D D+I T R N+
Sbjct: 39 LERLSTALLTITAVLIDAEEKQIT--NPVVEKWVNELRDVVYHAEDALDDIATEALRLNI 98
Query: 95 RMEKSSMAKVFSRSGPIF--------NIKLAHKVNSIREELNDIKSDMDVLNLK----FF 154
E SS ++ G + + L ++ + L + S ++L LK
Sbjct: 99 GAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMI 158
Query: 155 PAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKLIIGMLFDPKCEYNGEGEGEGISVI 214
P + LP T+S V ++ GR++D I+ L G+ GI+V+
Sbjct: 159 PKQRLP-----------TTSLVDESEVFGRDDDKDEIMRFLIPEN------GKDNGITVV 218
Query: 215 PIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRVYQAFT-RDKDSG 274
IVG GG+GKTTL+QL+YND+ V+ +F W V E D + ++VY++ T R +
Sbjct: 219 AIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFT 278
Query: 275 RLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLLRL-LECGRKGSKVIVTTRSLT 334
L V ++K R + +LLVLDD+W+E+ W DLLR +GS+++VTTRS
Sbjct: 279 DLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW-DLLRQPFIHAAQGSQILVTTRSQR 338
Query: 335 VAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERNPDLTRIGKEIVDSCGNVPLAI 394
VA ++ + L L++ + W+LF K F + + N ++ + + IV C +PLA+
Sbjct: 339 VASIMCAVHV-HNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAV 398
Query: 395 RVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSYDYLPPALKQCFAYCSLF 454
+ +G +L + EWER+ +S++ + + L+ VL++SY YLP LK+CFAYCS+F
Sbjct: 399 KTLGGVLRFEGKVIEWERVLSSRIWDLPADKSN-LLPVLRVSYYYLPAHLKRCFAYCSIF 458
Query: 455 PKDYRYEKANLVHLWMAQGYFEPS----NNEDIGGQYFSELLGRNLFQDPEQDYRGIETY 514
PK + +EK +V LWMA+G+ + + N E++G +YFSEL R+L Q + Y
Sbjct: 459 PKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK------TRY 518
Query: 515 KMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGGENIELNEPLIREFRHVRLG 574
MHD +++LA F +G + ED G ++++E R ++R
Sbjct: 519 IMHDFINELAQFASG---------EFSSKFED--------GCKLQVSE-RTRYLSYLRDN 578
Query: 575 VTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQALSLERVVLKCKSLRVLDFSYMEI 634
+ + K + T L S L+Q +S E+++ LRVL S+ +I
Sbjct: 579 YAEPMEFEALREVKFLRTFLPLSLTNSSR-SCCLDQMVS-EKLLPTLTRLRVLSLSHYKI 638
Query: 635 TRIPNSIGK-LVHLRYLNLQHTHIECLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTN 694
R+P K + H R+L+L T +E LP ++ + NLQTL + C LKELP + L N
Sbjct: 639 ARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLIN 698
Query: 695 LRYLINSKSGLTDLPSGFGKLTCLQELSKFMI--GEGNGIDSLPAL-NLVGNLDI-EFNK 754
LRYL + L +P FG+L LQ L+ F + +G+ I L L +L G L I E +
Sbjct: 699 LRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQR 758
Query: 755 WGSESVLEAKGANLKNNHQLTCLKF-----SFNFEMEEAALGEMDKMLMCLQLPP--NLE 814
V +A ANL + L + F S + E ++ + +L P ++E
Sbjct: 759 --VVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIE 818
Query: 815 KLEVYLYKGVEMPRRWLD--GLDKLISITIRECNNCRVVPHMSRLLHLKYLRLKFLDTLE 874
KL + YKG P WL +++ I +REC C +P + +L LK L + + L+
Sbjct: 819 KLAIERYKGRRFP-DWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQ 878
Query: 875 ------YVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLLNLE 934
Y D + + F SLE L NL + + W G+ LFP L L
Sbjct: 879 SIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGD----LFPSLKKLF 927
Query: 935 VSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYKGVE 945
+ CP+L L L SL + +I ++ L + Y Y+ ++
Sbjct: 939 ILRCPELTG-TLPTFLPSLISLHI----YKCGLLDFQPDHHEYSYRNLQ 927
BLAST of Spo25618.1 vs. TAIR (Arabidopsis)
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 339.3 bits (869), Expect = 1.100e-92
Identity = 287/949 (30.24%), Postives = 461/949 (48.58%), Query Frame = 1
Query: 35 LKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQVLYRADDLFDEIITLDRR-NL 94
L++L + I A + DAE +Q N + W++ L+ V+Y A+D D+I T R N+
Sbjct: 39 LERLSTALLTITAVLIDAEEKQIT--NPVVEKWVNELRDVVYHAEDALDDIATEALRLNI 98
Query: 95 RMEKSSMAKVFSRSGPIF--------NIKLAHKVNSIREELNDIKSDMDVLNLK----FF 154
E SS ++ G + + L ++ + L + S ++L LK
Sbjct: 99 GAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMI 158
Query: 155 PAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKLIIGMLFDPKCEYNGEGEGEGISVI 214
P + LP T+S V ++ GR++D I+ L G+ GI+V+
Sbjct: 159 PKQRLP-----------TTSLVDESEVFGRDDDKDEIMRFLIPEN------GKDNGITVV 218
Query: 215 PIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRVYQAFT-RDKDSG 274
IVG GG+GKTTL+QL+YND+ V+ +F W V E D + ++VY++ T R +
Sbjct: 219 AIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFT 278
Query: 275 RLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLLRL-LECGRKGSKVIVTTRSLT 334
L V ++K R + +LLVLDD+W+E+ W DLLR +GS+++VTTRS
Sbjct: 279 DLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW-DLLRQPFIHAAQGSQILVTTRSQR 338
Query: 335 VAGVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERNPDLTRIGKEIVDSCGNVPLAI 394
VA ++ + L L++ + W+LF K F + + N ++ + + IV C +PLA+
Sbjct: 339 VASIMCAVHV-HNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAV 398
Query: 395 RVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSYDYLPPALKQCFAYCSLF 454
+ +G +L + EWER+ +S++ + + L+ VL++SY YLP LK+CFAYCS+F
Sbjct: 399 KTLGGVLRFEGKVIEWERVLSSRIWDLPADKSN-LLPVLRVSYYYLPAHLKRCFAYCSIF 458
Query: 455 PKDYRYEKANLVHLWMAQGYFEPS----NNEDIGGQYFSELLGRNLFQDPEQDYRGIETY 514
PK + +EK +V LWMA+G+ + + N E++G +YFSEL R+L Q + Y
Sbjct: 459 PKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK------TRY 518
Query: 515 KMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGGENIELNEPLIREFRHVRLG 574
MHD +++LA F +G + ED G ++++E R ++R
Sbjct: 519 IMHDFINELAQFASG---------EFSSKFED--------GCKLQVSE-RTRYLSYLRDN 578
Query: 575 VTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQALSLERVVLKCKSLRVLDFSYMEI 634
+ + K + T L S L+Q +S E+++ LRVL S+ +I
Sbjct: 579 YAEPMEFEALREVKFLRTFLPLSLTNSSR-SCCLDQMVS-EKLLPTLTRLRVLSLSHYKI 638
Query: 635 TRIPNSIGK-LVHLRYLNLQHTHIECLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTN 694
R+P K + H R+L+L T +E LP ++ + NLQTL + C LKELP + L N
Sbjct: 639 ARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLIN 698
Query: 695 LRYLINSKSGLTDLPSGFGKLTCLQELSKFMI--GEGNGIDSLPAL-NLVGNLDI-EFNK 754
LRYL + L +P FG+L LQ L+ F + +G+ I L L +L G L I E +
Sbjct: 699 LRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQR 758
Query: 755 WGSESVLEAKGANLKNNHQLTCLKF-----SFNFEMEEAALGEMDKMLMCLQLPP--NLE 814
V +A ANL + L + F S + E ++ + +L P ++E
Sbjct: 759 --VVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIE 818
Query: 815 KLEVYLYKGVEMPRRWLD--GLDKLISITIRECNNCRVVPHMSRLLHLKYLRLKFLDTLE 874
KL + YKG P WL +++ I +REC C +P + +L LK L + + L+
Sbjct: 819 KLAIERYKGRRFP-DWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQ 878
Query: 875 ------YVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLLNLE 934
Y D + + F SLE L NL + + W G+ LFP L L
Sbjct: 879 SIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGD----LFPSLKKLF 927
Query: 935 VSECPKLMSMPLAPMLESLEACNIHGTLFESNLTSVVDEEEVYLYKGVE 945
+ CP+L L L SL + +I ++ L + Y Y+ ++
Sbjct: 939 ILRCPELTG-TLPTFLPSLISLHI----YKCGLLDFQPDHHEYSYRNLQ 927
BLAST of Spo25618.1 vs. TAIR (Arabidopsis)
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 330.9 bits (847), Expect = 3.800e-90
Identity = 271/897 (30.21%), Postives = 423/897 (47.16%), Query Frame = 1
Query: 35 LKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQVLYRADDLFDEIITLD-RRNL 94
LK+L+ + ++ A A+ Q AE+ + WL+ +K ++A+D+ DE+ T RR +
Sbjct: 36 LKRLK--VALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRV 95
Query: 95 RMEKSSMAKVFSR---SGPIFNIKLAHKVNSIREELNDIKSDMDVLNLKFFPAEELPPLM 154
E + +F K+ K+ + L ++V+ LK + +E P
Sbjct: 96 VAEAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKEY-SETREPQW 155
Query: 155 MRLIKERQTSSFVKTEDIIGRENDTKLIIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLG 214
+ + R + ++GR D ++ +L G+ +VI +VG G+G
Sbjct: 156 RQASRSRPDD--LPQGRLVGRVEDKLALVNLLLSDDEISIGKP-----AVISVVGMPGVG 215
Query: 215 KTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRVYQAFTRDKDSGRLLVDEIKRG 274
KTTL ++V+ND V +HF+V W+ + V + V Q T + L ++
Sbjct: 216 KTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDL-PSLQIQ 275
Query: 275 IRQSINNNKYLLVLDDIWDESRDRWLDLLRLLECGRKGSKVIVTTRSLTVAGVVGTKEAT 334
++++++ ++LLVLDD W ES W +GSK+++TTRS + V E
Sbjct: 276 LKKTLSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTRS-EIVSTVAKAEKI 335
Query: 335 YKLGLLTEEESWNLFKKFAFES-GQEERNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSK 394
Y++ L+T EE W L +FAF + N +L IGK I + C +PLA R + S L SK
Sbjct: 336 YQMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSK 395
Query: 395 YSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKAN 454
+ +W + SK + ++ VLKLSYD LPP LK+CFA CS+FPK + +++
Sbjct: 396 PNPDDWYAV-----SKNFSSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREE 455
Query: 455 LVHLWMAQGY-FEPSNN---EDIGGQYFSELLGRNLFQDPEQDYRGIETYKMHDLVHDLA 514
LV LWMA ++P ++ EDIG Y +L+ ++ FQ + + ++ MHDL++DLA
Sbjct: 456 LVLLWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRLDIT---MTSFVMHDLMNDLA 515
Query: 515 LFVAGGEIMELQGQDAEDDDEDYMVLVGAGGENIELNEPLIREFRHVRLGVTKGALSDGD 574
V+G L EDD NI R F R + D
Sbjct: 516 KAVSGDFCFRL-----EDD-------------NIPEIPSTTRHFSFSR------SQCDAS 575
Query: 575 DVFKAVTTLLNARRMRSLLFDMGLEQALSLERVVLKC----KSLRVLDFSYMEITRIPNS 634
F+++ R + LE E+V+ LR+L S+ +IT +P S
Sbjct: 576 VAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKS 635
Query: 635 IGKLVHLRYLNLQHTHIECLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYLINS 694
+ L LRYL+L T I+ LP+ + L NLQTL + CR L LP++ +L NLR L
Sbjct: 636 LKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLV 695
Query: 695 KSGLTDLPSGFGKLTCLQELSKFMIG--EGNGIDSLPAL-NLVGNLDI-EFNKWGSESVL 754
+ L ++P G KL LQ+LS F+IG G G+ L L +L G L I E S
Sbjct: 696 GTPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFAS-- 755
Query: 755 EAKGANLKNNHQLTCLKFSFNFEME-------EAALGEMDKMLMCLQLPPNLEKLEVYLY 814
EAK A LK L L + + A + ++L L+ P+L+ + Y
Sbjct: 756 EAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESY 815
Query: 815 KGVEMPRRWL--DGLDKLISITIRECNNCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDI 874
+G P +WL + S+T+ CN C +P + +L LKYL ++ + L+ V D
Sbjct: 816 QGGAFP-KWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFF 875
Query: 875 WVGNN---VYFPSLEGLTLQNLRELKGWTRPSKDDGEPRQLLFPCLLNLEVSECPKL 903
+ NN V F SL+ L + W P +DG +FPCL L + CP L
Sbjct: 876 FGENNSRGVPFQSLQILKFYGMPRWDEWICPELEDG-----IFPCLQKLIIQRCPSL 880
BLAST of Spo25618.1 vs. TAIR (Arabidopsis)
Match:
AT3G46730.1 (NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 238.8 bits (608), Expect = 2.000e-62
Identity = 222/828 (26.81%), Postives = 376/828 (45.41%), Query Frame = 1
Query: 14 LESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAENNESINLWLSRLKQ 73
L + + E + G + DL++L+ + I ++D E R+ + E W +
Sbjct: 10 LNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEARERED--EVSKEWTKLVLD 69
Query: 74 VLYRADDLFDE-IITLDRRNLRMEKSSMAKVFSRSGPIFNIKLAHKVNSIREELNDIKSD 133
+ Y +D+ D + L+ R+LR + + +NI + +++ + DI
Sbjct: 70 IAYDIEDVLDTYFLKLEERSLRRGLLRLTNKIGKKRDAYNI--VEDIRTLKRRILDITRK 129
Query: 134 MDVLNLKFFP---AEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKLIIGMLFDPKCEY 193
+ + F E + + +R + R+ + E ++G E+D K+++ L
Sbjct: 130 RETFGIGSFNEPRGENITNVRVRQL--RRAPPVDQEELVVGLEDDVKILLVKLLS----- 189
Query: 194 NGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQDAVMRRV 253
+ E + +I I G GGLGKT LA+ +YN V++ FD W V + ++ R+
Sbjct: 190 --DNEKDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRI 249
Query: 254 YQAF----TRDKDSGRLLVD--EIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLLRLLE 313
++ + + ++ + E++ + + Y++V+DD+WD D W L R L
Sbjct: 250 IRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDP--DAWESLKRALP 309
Query: 314 CGRKGSKVIVTTRSLTVA-GVVGTKEATYKLGLLTEEESWNLFKKFAFESGQEERNPDLT 373
C +GSKVI+TTR +A GV GT A +KL LT EESW LF++ AF S E+ + DL
Sbjct: 310 CDHRGSKVIITTRIRAIAEGVEGTVYA-HKLRFLTFEESWTLFERKAF-SNIEKVDEDLQ 369
Query: 374 RIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIRNSQLSKAKLKEGDRLMAVLKLSY 433
R GKE+V CG +PLAI V+ LL S+ EW + S + K + V LS+
Sbjct: 370 RTGKEMVKKCGGLPLAIVVLSGLL-SRKRTNEWHEVCASLWRRLK-DNSIHISTVFDLSF 429
Query: 434 DYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNN---EDIGGQYFSELLGR 493
+ LK CF Y S+FP+DY + L+HL +A+G+ + ED+ Y EL+ R
Sbjct: 430 KEMRHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCYIDELVDR 489
Query: 494 NLFQDPEQDYRGIETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDEDYMVLVGAGGENI 553
+L + + + + ++HDL+ DLA+ A EL V E
Sbjct: 490 SLVKAERIERGKVMSCRIHDLLRDLAIKKA----KELN-------------FVNVYNEKQ 549
Query: 554 ELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTLLNARRMRSLLFDMGLEQALS-LERV 613
++ RE H + +D + + +RMRS LF +G + +
Sbjct: 550 HSSDICRREVVHHLM----------NDYYLCDRRV--NKRMRSFLF-IGERRGFGYVNTT 609
Query: 614 VLKCKSLRVLDFSYMEI------TRIPNSIGKLVHLRYLNLQHTHIECLPDAITKLDNLQ 673
LK K LRVL+ + +P+ IG+L+HLRYL + T++ LP +I+ L LQ
Sbjct: 610 NLKLKLLRVLNMEGLLFVSKNISNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQ 669
Query: 674 TLNIMGCRRLKELPRNFGKLTNLRYLINSKSGLTDLPSGFGKLTCLQELSKFMIGEGNGI 733
TL+ G + + KLT+LR++I G + G L L+ +S + + N
Sbjct: 670 TLDASGNDPF-QYTTDLSKLTSLRHVIGKFVGECLIGEGV-NLQTLRSISSYSWSKLNH- 729
Query: 734 DSLPALNLVGNLDI-EFNKWGSESVLEAKGANLKNNHQLTCLKFSF-NFEM---EEAALG 793
L + +L+I + +KW + + + L LK NF++ +G
Sbjct: 730 ---ELLRNLQDLEIYDHSKWVDQRRVPLNFVSFSKPKNLRVLKLEMRNFKLSSESRTTIG 776
Query: 794 EMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECN 816
+D P+LE L + E L L +L + +++CN
Sbjct: 790 LVDVNF------PSLESLTLVGTTLEENSMPALQKLPRLEDLVLKDCN 776
BLAST of Spo25618.1 vs. TAIR (Arabidopsis)
Match:
AT1G58390.1 (Disease resistance protein (CC-NBS-LRR class) family)
HSP 1 Score: 238.4 bits (607), Expect = 2.600e-62
Identity = 239/936 (25.53%), Postives = 416/936 (44.44%), Query Frame = 1
Query: 8 ELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAENNESINLW 67
EL S ++ L +E + G + L+ + ++ + ++DA+ ++ +
Sbjct: 4 ELVSFGIKKLWDLLSQECEQFQGVEDQVTGLKRDLNLLSSFLKDADAKKHT--TAVVRNV 63
Query: 68 LSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKVFSRSGPIF---NIKLAHKVNSIRE 127
+ +K+++Y A+D+ + + ++ + K+S K+ R + A V IR
Sbjct: 64 VEEIKEIVYDAEDIIETYLLKEK----LWKTSGIKMRIRRHACIISDRRRNALDVGGIRT 123
Query: 128 ELNDIKSDMDVLNLK--FFPAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKLIIGML 187
++D+ DM ++ + P R + RQT S D +G E + K ++G L
Sbjct: 124 RISDVIRDMQSFGVQQAIVDGGYMQPQGDRQREMRQTFSKDYESDFVGLEVNVKKLVGYL 183
Query: 188 FDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVPEVDDQ 247
D E + V+ I G GGLGKTTLA+ V+N E V+ FD WVCV + +
Sbjct: 184 VDE----------ENVQVVSITGMGGLGKTTLARQVFNHEDVKHQFDRLAWVCVSQEFTR 243
Query: 248 DAVMRRVYQAFT-RDKDSGRLLVD--EIKRGIRQSINNNKYLLVLDDIWDESRDRWLDLL 307
V + + Q T R+K L ++ E+ + Q + +K L+V DDIW +D DL+
Sbjct: 244 KNVWQMILQNLTSREKKDEILQMEEAELHDKLFQLLETSKSLIVFDDIW---KDEDWDLI 303
Query: 308 RLLECGRKGSKVIVTTRSLTVAGVVGTKEATYKLGLLTEEESWNLFKKFAF---ESGQEE 367
+ + KG KV++T+++ +VA K +K L E+SW LF++ AF ++ + +
Sbjct: 304 KPIFPPNKGWKVLLTSQNESVAVRGDIKYLNFKPECLAIEDSWTLFQRIAFPKKDASESK 363
Query: 368 RNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSKYSEKEWERIR---NSQLSKAKLKEGDR 427
+ ++ +GK+++ CG +PLAI+V+G LL +KY+ +WER+ S +
Sbjct: 364 VDEEMEDMGKQMLKHCGGLPLAIKVLGGLLAAKYTMHDWERLSVNIGSDIVGRTSSNNSS 423
Query: 428 LMAVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQG------YFEPSNNED 487
+ VL +S++ LP LK CF Y + FP+D++ L + W A+G Y +D
Sbjct: 424 IYHVLSMSFEELPSYLKHCFLYLAHFPEDHKINVEKLSYCWAAEGISTAEDYHNGETIQD 483
Query: 488 IGGQYFSELLGRN-LFQDPEQDYRGIETYKMHDLVHDLALFVAGGEIMELQGQDAEDDDE 547
+G Y EL+ RN + + + T +HD++ ++ LF A ++
Sbjct: 484 VGQSYLEELVRRNMIIWERDATASRFGTCHLHDMMREVCLFKA-------------KEEN 543
Query: 548 DYMVLVGAGGENIELNEPLIREFRHVRLGVTKGALSDGDDVFKAVTTL-----LNARRMR 607
+ V + G R RL V++ TTL +N ++R
Sbjct: 544 FLQIAVKSVGVTSSSTGNSQSPCRSRRL------------VYQCPTTLHVERDINNPKLR 603
Query: 608 SLL---FDMGLEQALSLERVVLKCKSLRVLDFSYMEI--TRIPNSIGKLVHLRYLNLQHT 667
SL+ D+ +E L + K LRVLD Y++ ++P IG L+HLRYL+LQ
Sbjct: 604 SLVVLWHDLWVENWKLLGTSFTRLKLLRVLDLFYVDFEGMKLPFGIGNLIHLRYLSLQDA 663
Query: 668 HIECLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYL-----INSKSGLTDLPSG 727
+ LP ++ L L LN+ +P F ++ LRYL ++ K+ L+
Sbjct: 664 KVSHLPSSLGNLMLLIYLNLDVDTEFIFVPDVFMRMHELRYLKLPLHMHKKTRLS----- 723
Query: 728 FGKLTCLQELSKFMIGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLT 787
L L+ L F + D L + + L I + S L A + L+N
Sbjct: 724 LRNLVKLETLVYFSTWHSSSKD-LCGMTRLMTLAIRLTRVTSTETLSASISGLRN----- 783
Query: 788 CLKFSFNFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITI 847
L++ + + E +L + L L L++Y+ + P R L + +
Sbjct: 784 -LEYLYIVGTHSKKMREEGIVLDFIHLKHLL--LDLYMPRQQHFPSR-------LTFVKL 843
Query: 848 RECN-NCRVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLREL 907
EC +P + +LLHLK + L Y + G FP L+ L + L +
Sbjct: 844 SECGLEEDPMPILEKLLHLKGV---ILLKGSYCGRRMVCSGGG--FPQLKKLEIVGLNKW 861
BLAST of Spo25618.1 vs. TAIR (Arabidopsis)
Match:
AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1)
HSP 1 Score: 233.4 bits (594), Expect = 8.200e-61
Identity = 250/930 (26.88%), Postives = 404/930 (43.44%), Query Frame = 1
Query: 1 MAEGIVWELASKALESLSKAALEEGSSWWGARSDLKKLEDTIRMIQARVRDAERRQEAEN 60
M + +V K L L E+G + R L+ L+ ++ +Q+ ++DAER++
Sbjct: 1 MVDAVVTVFLEKTLNILE----EKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRT-- 60
Query: 61 NESINLWLSRLKQVLYRADDLFDEIITLDRRNLRMEKSSMAKVFSRSGPI---FNIKLAH 120
NE++ ++ L++++Y A+D+ + D + ++SS A + SR P K +
Sbjct: 61 NETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWL-SRLHPARVPLQYKKSK 120
Query: 121 KVNSIREELNDIKSDMDVLNLKFFPAEELPPLMMRLIKERQTSSFVKTEDIIGRENDTKL 180
++ I E + IKS ++ +F + +R +S ++G E D +
Sbjct: 121 RLQEINERITKIKSQVE----PYFEFITPSNVGRDNGTDRWSSPVYDHTQVVGLEGDKRK 180
Query: 181 IIGMLFDPKCEYNGEGEGEGISVIPIVGFGGLGKTTLAQLVYNDEAVQKHFDVTTWVCVP 240
I LF + ++ VG GGLGKTT+AQ V+ND+ ++ F+ WV V
Sbjct: 181 IKEWLF--------RSNDSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVS 240
Query: 241 EVDDQDAVMRRVYQAF---TRDKDSGRLLVDEIKRGIRQSINNNKYLLVLDDIWDESRDR 300
+ ++ +MR + + + D G LL R I+Q + +YL+V+DD+WD++
Sbjct: 241 QTFTEEQIMRSILRNLGDASVGDDIGTLL-----RKIQQYLLGKRYLIVMDDVWDKNLSW 300
Query: 301 WLDLLRLLECGRKGSKVIVTTRSLTVAGVVGTK-EATYKLGLLTEEESWNLFKKFAF--E 360
W + + L G+ GS VIVTTRS +VA V + + T++ LL+ + SW LF AF
Sbjct: 301 WDKIYQGLPRGQGGS-VIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAAN 360
Query: 361 SGQEERNPDLTRIGKEIVDSCGNVPLAIRVVGSLLYSK-YSEKEWERIRN--SQLSKAKL 420
G ER P+L +GKEIV C +PL I+ VG LL K + EW RI +
Sbjct: 361 DGTCER-PELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNT 420
Query: 421 KEGDRLMAVLKLSYDYLPPALKQCFAYCSLFPKDYRYEKANLVHLWMAQGYFEPSNNE-- 480
E D +M+ L+LSYD LP LK C SL+P+D K LVH W+ +G+ N
Sbjct: 421 SETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSA 480
Query: 481 -DIGGQYFSELLGRNLFQDPEQDYRG-IETYKMHDLVHDLALFVAGGEIMELQGQDAEDD 540
+ G FS L R L + ++ Y G I T K+HD+V DL ++++ +D+ +
Sbjct: 481 TESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDL--------VIDIAKKDSFSN 540
Query: 541 DEDYMVL-VGAGGENIELNEPLIREFRHVRLGVTKGALSD-GDDVFKAVT--TLLNARRM 600
E +G G E + + R V G ++ D+ K T L +
Sbjct: 541 PEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDI 600
Query: 601 RSLLFDMGLEQALSLERVVLKCKSLRVLDFSYMEITRIPNSIGKLVHLRYLNLQHTH-IE 660
+FD L + L + I L HL L+L +TH +
Sbjct: 601 SKSIFDAPLSEIL--------------------------DEIASLQHLACLSLSNTHPLI 660
Query: 661 CLPDAITKLDNLQTLNIMGCRRLKELPRNFGKLTNLRYLINSKSG-LTDLPSGFGKLTCL 720
P ++ L NLQ L+ C+ LK+L L L + G L P G G L L
Sbjct: 661 QFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKL 720
Query: 721 QELSKFM-IGEGNGIDSLPALNLVGNLDIEFNKWGSESVLEAKGANLKNNHQLTCLKFSF 780
+ L F NG NL + + + + E + +L N +L + +
Sbjct: 721 EVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINC 780
Query: 781 NFEMEEAALGEMDKMLMCLQLPPNLEKLEVYLYKGVEMPRRWLDGLDKLISITIRECNNC 840
+ + ++D L P L +L + Y G P WL
Sbjct: 781 YDSYGDDLITKID----ALTPPHQLHELSLQFYPGKSSP-SWLS---------------- 834
Query: 841 RVVPHMSRLLHLKYLRLKFLDTLEYVEDDDIWVGNNVYFPSLEGLTLQNLRELKGWTRPS 900
PH +L L+Y+ + + ++ E W N ++ +EGL L +L +L
Sbjct: 841 ---PH--KLPMLRYMSICSGNLVKMQE--PFWGNENTHW-RIEGLMLSSLSDL------- 834
Query: 901 KDDGEPRQLLFPCLLNLEVSECPKLMSMPL 908
D E Q P L + + CP+L S +
Sbjct: 901 DMDWEVLQQSMPYLRTVTANWCPELESFAI 834
The following BLAST results are available for this feature:
