Spo10769 (gene) | SpinachBase

Overview


Name	Spo10769
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	chr1 : 41363990 .. 41364607 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCCATTACTTCCTCTCAGCAGCCACCATCCTCCTATTCTTATCCCTTTCTCCACCCACCACCACCGCTGCCAACCCCGCCGTCCTCGACATCAATGGCCACCCTCTTCGCCCGCGATCCAACTACATCCTCCCAGTAGTCCGCGGCCGAGGCGGTGGGCTAACAATGTTATCAAAGAACACCACTGAAGTATGTCCACTCTACGTAGCACAAGAAAACCATGAAGTCTCAAATGGCCTACCTCTTAAGTTCTACTCGGTCAACCCTAAAGACACAAAGATATTTGTCTCAACTGACCTCAACTTCGTCTTCGATGCCTCCACCATCTGCGTTCAATCCAACACTTGGGAGTTAACCATCGATGAAGAGACCGGAAGGCGGTACATTGGTGTCGGAGGAGAAATCGGTAATCCTGGAGTGCAAACGGTTAGTAACTGGTTTAAGATAGAGAAGGCGGGAAGTGGGAAGTATGATTATAAGATTGTGTATTGTGCTGGTGTTTGTAACTTTTGTAAGATTATGTGTGGAGATATTGGTGCTTTTGTTGAGGAAGATGGGAGGAGGTTGTTGGGTTTTAGTGGTCAGCCTCTTTTGGTTAGGTTCAAGAAAGCTTAA

mRNA sequence

ATGATCCATTACTTCCTCTCAGCAGCCACCATCCTCCTATTCTTATCCCTTTCTCCACCCACCACCACCGCTGCCAACCCCGCCGTCCTCGACATCAATGGCCACCCTCTTCGCCCGCGATCCAACTACATCCTCCCAGTAGTCCGCGGCCGAGGCGGTGGGCTAACAATGTTATCAAAGAACACCACTGAAGTATGTCCACTCTACGTAGCACAAGAAAACCATGAAGTCTCAAATGGCCTACCTCTTAAGTTCTACTCGGTCAACCCTAAAGACACAAAGATATTTGTCTCAACTGACCTCAACTTCGTCTTCGATGCCTCCACCATCTGCGTTCAATCCAACACTTGGGAGTTAACCATCGATGAAGAGACCGGAAGGCGGTACATTGGTGTCGGAGGAGAAATCGGTAATCCTGGAGTGCAAACGGTTAGTAACTGGTTTAAGATAGAGAAGGCGGGAAGTGGGAAGTATGATTATAAGATTGTGTATTGTGCTGGTGTTTGTAACTTTTGTAAGATTATGTGTGGAGATATTGGTGCTTTTGTTGAGGAAGATGGGAGGAGGTTGTTGGGTTTTAGTGGTCAGCCTCTTTTGGTTAGGTTCAAGAAAGCTTAA

Coding sequence (CDS)

ATGATCCATTACTTCCTCTCAGCAGCCACCATCCTCCTATTCTTATCCCTTTCTCCACCCACCACCACCGCTGCCAACCCCGCCGTCCTCGACATCAATGGCCACCCTCTTCGCCCGCGATCCAACTACATCCTCCCAGTAGTCCGCGGCCGAGGCGGTGGGCTAACAATGTTATCAAAGAACACCACTGAAGTATGTCCACTCTACGTAGCACAAGAAAACCATGAAGTCTCAAATGGCCTACCTCTTAAGTTCTACTCGGTCAACCCTAAAGACACAAAGATATTTGTCTCAACTGACCTCAACTTCGTCTTCGATGCCTCCACCATCTGCGTTCAATCCAACACTTGGGAGTTAACCATCGATGAAGAGACCGGAAGGCGGTACATTGGTGTCGGAGGAGAAATCGGTAATCCTGGAGTGCAAACGGTTAGTAACTGGTTTAAGATAGAGAAGGCGGGAAGTGGGAAGTATGATTATAAGATTGTGTATTGTGCTGGTGTTTGTAACTTTTGTAAGATTATGTGTGGAGATATTGGTGCTTTTGTTGAGGAAGATGGGAGGAGGTTGTTGGGTTTTAGTGGTCAGCCTCTTTTGGTTAGGTTCAAGAAAGCTTAA

Protein sequence

MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNYILPVVRGRGGGLTMLSKNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFVEEDGRRLLGFSGQPLLVRFKKA

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo10769.1	Spo10769.1	mRNA

Homology

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BLAST of Spo10769.1 vs. NCBI nr
Match: gi|902194163|gb|KNA12470.1| (hypothetical protein SOVF_125760 [Spinacia oleracea])

HSP 1 Score: 419.1 bits (1076), Expect = 4.500e-114
Identity = 205/206 (99.51%), Postives = 205/206 (99.51%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSN-YILPVVRGRGGGLTMLS 60
           MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSN YILPVVRGRGGGLTMLS
Sbjct: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNYYILPVVRGRGGGLTMLS 60

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120
           KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL
Sbjct: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120

Query: 121 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 180
           TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI
Sbjct: 121 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 180

Query: 181 GAFVEEDGRRLLGFSGQPLLVRFKKA 206
           GAFVEEDGRRLLGFSGQPLLVRFKKA
Sbjct: 181 GAFVEEDGRRLLGFSGQPLLVRFKKA 206

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BLAST of Spo10769.1 vs. NCBI nr
Match: gi|731344031|ref|XP_010683193.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 332.8 bits (852), Expect = 4.200e-88
Identity = 159/206 (77.18%), Postives = 176/206 (85.44%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSN-YILPVVRGRGGGLTMLS 60
           M H  +SAATI LFL LSP T+TA N AVLDING PL+   N YILPV+RGRGGGLTM S
Sbjct: 1   MTHVIISAATIFLFLYLSPLTSTADNTAVLDINGRPLQAGYNYYILPVIRGRGGGLTMAS 60

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120
           KN TE+CPLYVAQE+HEVSNGLPLKFY VNPKD +I +S+DLNFVFDA+T CVQS  W L
Sbjct: 61  KNATELCPLYVAQEDHEVSNGLPLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTGWSL 120

Query: 121 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 180
           TI+ ETGRRY+G GGEIGNPGV+TV NWFKIEK GSGKYDYKIVYC GVCNFCK+MCGD+
Sbjct: 121 TIEMETGRRYVGTGGEIGNPGVETVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMCGDV 180

Query: 181 GAFVEEDGRRLLGFSGQPLLVRFKKA 206
           G F+E+DGRRLLGFS QPLLV FKKA
Sbjct: 181 GVFIEKDGRRLLGFSDQPLLVMFKKA 206

[Close]

BLAST of Spo10769.1 vs. NCBI nr
Match: gi|731344033|ref|XP_010683194.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 309.3 bits (791), Expect = 5.000e-81
Identity = 152/202 (75.25%), Postives = 168/202 (83.17%), Query Frame = 1


		  
Query: 6   LSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLSKNTTE 65
           LSAATI LFL LSP T+TAAN AVLDING PL+ RSNY ILPV+RGRGGGL M  KN T+
Sbjct: 7   LSAATIFLFLCLSPLTSTAANIAVLDINGRPLQARSNYYILPVIRGRGGGLRMTPKNATQ 66

Query: 66  VCPL-YVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWELTIDE 125
           +CPL YVAQE  E++NGLPLKFY VNPKD  I +STDLNFVFDA+TICVQS  W L  DE
Sbjct: 67  LCPLAYVAQEGSELANGLPLKFYPVNPKDKTISLSTDLNFVFDAATICVQSTQWRLAFDE 126

Query: 126 ETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFV 185
            TGRRY+G GGEIGNPG  TVSNWFKIEKA +GKYDYKIV+C GVCNFCK+ CG+IG FV
Sbjct: 127 VTGRRYVGFGGEIGNPGGNTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKVACGEIGVFV 186

Query: 186 EEDGRRLLGFSGQPLLVRFKKA 206
           E+DGRRLLG + QPLLV FKKA
Sbjct: 187 EKDGRRLLGINNQPLLVMFKKA 208

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BLAST of Spo10769.1 vs. NCBI nr
Match: gi|902082350|gb|KNA03166.1| (hypothetical protein SOVF_211800 [Spinacia oleracea])

HSP 1 Score: 290.4 bits (742), Expect = 2.400e-75
Identity = 138/209 (66.03%), Postives = 164/209 (78.47%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLS 60
           M H+ LS + I LF SLS  TTTAANPAVLDI+GHPL   SNY ILPVVRGRGGGLT+  
Sbjct: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNT--- 120
           +N TE+CPL  AQ++ EV +G+PL+ Y VNP D  ++VSTDLN VFDA  +CV S     
Sbjct: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120

Query: 121 WELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMC 180
           W+LT DE TGRRY+G GG+ GNPG++TVSNWFKI+KAGSG++DYKIV+C  VCN CK+MC
Sbjct: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180

Query: 181 GDIGAFVEEDGRRLLGFSGQPLLVRFKKA 206
           GD+G FVE DGRRLLG   QPLLVRFK+A
Sbjct: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 209

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BLAST of Spo10769.1 vs. NCBI nr
Match: gi|902170260|gb|KNA07819.1| (hypothetical protein SOVF_168350 [Spinacia oleracea])

HSP 1 Score: 275.0 bits (702), Expect = 1.000e-70
Identity = 131/208 (62.98%), Postives = 158/208 (75.96%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPA--VLDINGHPLRPRSN-YILPVVRGRGGGLTM 60
           M ++ LSAAT L FL  SPP+TTAA     V DING PL+  S  YILPV+RGRGGGLTM
Sbjct: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60

Query: 61  LSKNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTW 120
              NTT+ CPLYVAQENHEVSNGLPLK Y VNP + ++ +  D+N VFDA++ICVQS  W
Sbjct: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120

Query: 121 ELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCG 180
            L  DE TG++++  GG IGNPGV T+SNWF+IEKAGSG YDYK+ +C GVC FC +MCG
Sbjct: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCTVMCG 180

Query: 181 DIGAFVEEDGRRLLGFSGQPLLVRFKKA 206
           D+G F+ EDG R LG + +PLLV+FKKA
Sbjct: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 208

[Close]

BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QYY3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_125760 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 3.100e-114
Identity = 205/206 (99.51%), Postives = 205/206 (99.51%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSN-YILPVVRGRGGGLTMLS 60
           MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSN YILPVVRGRGGGLTMLS
Sbjct: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNYYILPVVRGRGGGLTMLS 60

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120
           KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL
Sbjct: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120

Query: 121 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 180
           TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI
Sbjct: 121 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 180

Query: 181 GAFVEEDGRRLLGFSGQPLLVRFKKA 206
           GAFVEEDGRRLLGFSGQPLLVRFKKA
Sbjct: 181 GAFVEEDGRRLLGFSGQPLLVRFKKA 206

[Close]

BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0T9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g153990 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 3.000e-88
Identity = 159/206 (77.18%), Postives = 176/206 (85.44%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSN-YILPVVRGRGGGLTMLS 60
           M H  +SAATI LFL LSP T+TA N AVLDING PL+   N YILPV+RGRGGGLTM S
Sbjct: 1   MTHVIISAATIFLFLYLSPLTSTADNTAVLDINGRPLQAGYNYYILPVIRGRGGGLTMAS 60

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120
           KN TE+CPLYVAQE+HEVSNGLPLKFY VNPKD +I +S+DLNFVFDA+T CVQS  W L
Sbjct: 61  KNATELCPLYVAQEDHEVSNGLPLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTGWSL 120

Query: 121 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 180
           TI+ ETGRRY+G GGEIGNPGV+TV NWFKIEK GSGKYDYKIVYC GVCNFCK+MCGD+
Sbjct: 121 TIEMETGRRYVGTGGEIGNPGVETVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMCGDV 180

Query: 181 GAFVEEDGRRLLGFSGQPLLVRFKKA 206
           G F+E+DGRRLLGFS QPLLV FKKA
Sbjct: 181 GVFIEKDGRRLLGFSDQPLLVMFKKA 206

[Close]

BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C4X4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g154000 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 3.500e-81
Identity = 152/202 (75.25%), Postives = 168/202 (83.17%), Query Frame = 1


		  
Query: 6   LSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLSKNTTE 65
           LSAATI LFL LSP T+TAAN AVLDING PL+ RSNY ILPV+RGRGGGL M  KN T+
Sbjct: 7   LSAATIFLFLCLSPLTSTAANIAVLDINGRPLQARSNYYILPVIRGRGGGLRMTPKNATQ 66

Query: 66  VCPL-YVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWELTIDE 125
           +CPL YVAQE  E++NGLPLKFY VNPKD  I +STDLNFVFDA+TICVQS  W L  DE
Sbjct: 67  LCPLAYVAQEGSELANGLPLKFYPVNPKDKTISLSTDLNFVFDAATICVQSTQWRLAFDE 126

Query: 126 ETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFV 185
            TGRRY+G GGEIGNPG  TVSNWFKIEKA +GKYDYKIV+C GVCNFCK+ CG+IG FV
Sbjct: 127 VTGRRYVGFGGEIGNPGGNTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKVACGEIGVFV 186

Query: 186 EEDGRRLLGFSGQPLLVRFKKA 206
           E+DGRRLLG + QPLLV FKKA
Sbjct: 187 EKDGRRLLGINNQPLLVMFKKA 208

[Close]

BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9I8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_211800 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 1.700e-75
Identity = 138/209 (66.03%), Postives = 164/209 (78.47%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLS 60
           M H+ LS + I LF SLS  TTTAANPAVLDI+GHPL   SNY ILPVVRGRGGGLT+  
Sbjct: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNT--- 120
           +N TE+CPL  AQ++ EV +G+PL+ Y VNP D  ++VSTDLN VFDA  +CV S     
Sbjct: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120

Query: 121 WELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMC 180
           W+LT DE TGRRY+G GG+ GNPG++TVSNWFKI+KAGSG++DYKIV+C  VCN CK+MC
Sbjct: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180

Query: 181 GDIGAFVEEDGRRLLGFSGQPLLVRFKKA 206
           GD+G FVE DGRRLLG   QPLLVRFK+A
Sbjct: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 209

[Close]

BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QKP3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_168350 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 7.300e-71
Identity = 131/208 (62.98%), Postives = 158/208 (75.96%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPA--VLDINGHPLRPRSN-YILPVVRGRGGGLTM 60
           M ++ LSAAT L FL  SPP+TTAA     V DING PL+  S  YILPV+RGRGGGLTM
Sbjct: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60

Query: 61  LSKNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTW 120
              NTT+ CPLYVAQENHEVSNGLPLK Y VNP + ++ +  D+N VFDA++ICVQS  W
Sbjct: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120

Query: 121 ELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCG 180
            L  DE TG++++  GG IGNPGV T+SNWF+IEKAGSG YDYK+ +C GVC FC +MCG
Sbjct: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCTVMCG 180

Query: 181 DIGAFVEEDGRRLLGFSGQPLLVRFKKA 206
           D+G F+ EDG R LG + +PLLV+FKKA
Sbjct: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 208

[Close]

BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 159.5 bits (402), Expect = 4.000e-38
Identity = 92/208 (44.23%), Postives = 122/208 (58.65%), Query Frame = 1


		  
Query: 4   YFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTM--LSK 63
           +F+SA        L     +A NP VLDI+G  LR  +NY I+PV+R  GGGLT+   + 
Sbjct: 12  FFVSALLAAAANPLLSAADSAPNP-VLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTP 71

Query: 64  NTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTIC--VQSNTWE 123
           N T VCP  V Q   EV +  PL F+  NPK+  + VSTDLN  F A   C    S  W 
Sbjct: 72  NGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWR 131

Query: 124 LT-IDEETGRRYIGVGGEIGNPGVQTVSNWFKIEK-AGSGKYDYKIVYCAGVCNFCKIMC 183
           L   DE TG+ ++ +GG  GNPG +T+S+WFKIE+  GSG Y  K+V+C  VC  CK+ C
Sbjct: 132 LDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFY--KLVFCPTVCGSCKVKC 191

Query: 184 GDIGAFVEEDGRRLLGFSGQPLLVRFKK 205
           GD+G ++++ GRR L  S +P    F K
Sbjct: 192 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.600e-26
Identity = 75/207 (36.23%), Postives = 102/207 (49.28%), Query Frame = 1


		  
Query: 7   SAATILLFLSLSPP----TTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLSKN 66
           +A  +LLF   S         AAN  VLD +G  L+    Y +L  + G GGG   L + 
Sbjct: 5   TAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 64

Query: 67  TTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLN--FVFDASTICVQSNTWEL 126
           T + CP  V Q   ++ NG P+ F + + KD  + VSTD+N  FV     +C  S  W L
Sbjct: 65  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 124

Query: 127 -TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGD 186
              D   G+ ++   G  G PG  T+ +WFKIEKA  G   YK  +C  VC+ C  +C D
Sbjct: 125 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKA--GVLGYKFRFCPSVCDSCTTLCSD 184

Query: 187 IGAFVEEDGRRLLGFSGQPLLVRFKKA 206
           IG   ++DG+  L  S       FKKA
Sbjct: 185 IGRHSDDDGQIRLALSDNEWAWMFKKA 209

[Close]

BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 2.100e-23
Identity = 83/201 (41.29%), Postives = 103/201 (51.24%), Query Frame = 1


		  
Query: 11  ILLFLSLSPPTTTAANPAVLDINGHPLRPR-SNYILPVVRGRGGGLTMLSKNTTEVCPLY 70
           ILL L     + +AA P V D  GH L    S Y+LP   G GGGLTM  +     CPL 
Sbjct: 9   ILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRVLP--CPLL 68

Query: 71  VAQENHEVSNGLPLKFY----SVNPKDTKIFVSTDLNFVFDASTICVQSNTWELTIDEET 130
           VAQE  E   G P++F     +  P+D  I VSTD+   F+A+TICVQS  W +  +  T
Sbjct: 69  VAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDEPLT 128

Query: 131 GRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFVEE 190
           G R +  G  IG P      N F++EK G G   YK+V        C+  C D+G  V  
Sbjct: 129 GARRVVTGPLIG-PSPSGRENAFRVEKYGGG---YKLV-------SCRDSCQDLG--VSR 188

Query: 191 DGRRL-LGFSGQPLLVRFKKA 206
           DG R  LG S  P +V FKKA
Sbjct: 189 DGARAWLGASQPPHVVVFKKA 194

[Close]

BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 8.100e-23
Identity = 72/192 (37.50%), Postives = 96/192 (50.00%), Query Frame = 1


		  
Query: 6   LSAATILLFL-----SLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLS 65
           LS  T+  FL     +LS   +      VLD NG+P+ P   Y ILP +RG  GG   L 
Sbjct: 4   LSPLTLSFFLFVFITNLSLAFSNEDVEQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLG 63

Query: 66  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 125
           +     CP+ V Q+  EV NGLP+KF         IF  T +   +     C +S+ W +
Sbjct: 64  RTGDLTCPVTVLQDRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKWLV 123

Query: 126 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 185
            +D    +  +G+GG    PGVQT+S  FKIEK  SG + YK+    G C      C D+
Sbjct: 124 FVDNVIQKACVGIGGPENYPGVQTLSGLFKIEKHESG-FGYKL----GFCVKGSPTCLDV 183

Query: 186 GAF-VEEDGRRL 191
           G F  +EDGRRL
Sbjct: 184 GRFDNDEDGRRL 190

[Close]

BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
Match: PIP22_PEA (Kunitz-type trypsin inhibitor-like 2 protein OS=Pisum sativum GN=PIP20-2 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.000e-22
Identity = 67/186 (36.02%), Postives = 95/186 (51.08%), Query Frame = 1


		  
Query: 10  TILLFLSLSPPTTTAANP---AVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLSKNTTEV 69
           + LLF+ ++  +   +N     VLD+NG P+ P   Y ILP +RG  GG   L +     
Sbjct: 10  SFLLFVFITTLSLAFSNEDVEQVLDVNGKPIFPGGQYYILPAIRGPPGGGVRLGRTGDLT 69

Query: 70  CPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWELTIDEET 129
           CP+ V Q+  EV NGLP+KF         IF  T +   +     C +S+ W + +D   
Sbjct: 70  CPVTVLQDRREVKNGLPVKFVIPGISPGIIFTGTPIEIEYTKKPNCAKSSKWLVFVDNVI 129

Query: 130 GRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAF-VE 189
            +  +G+GG    PG+QT+S  FKIEK  SG + YK+    G C      C D+G F  +
Sbjct: 130 QKACVGIGGPENYPGIQTLSGLFKIEKHESG-FGYKL----GFCIKGSPTCLDVGRFDND 189

Query: 190 EDGRRL 191
           E GRRL
Sbjct: 190 EAGRRL 190

[Close]

BLAST of Spo10769.1 vs. TAIR (Arabidopsis)
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 160.6 bits (405), Expect = 1.000e-39
Identity = 102/207 (49.28%), Postives = 125/207 (60.39%), Query Frame = 1


		  
Query: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNY-ILPVVRGRGGGLTMLS 60
           +++ FL  A   +F+S    TT AA   V DING  L    NY ILPV+RGRGGGLTM S
Sbjct: 4   LLYIFLLLA---VFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTM-S 63

Query: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTWEL 120
              TE CP  V Q+  EVS GLP+KF S   K   I VSTD+N  F  ++I      WEL
Sbjct: 64  NLKTETCPTSVIQDQFEVSQGLPVKF-SPYDKSRTIPVSTDVNIKFSPTSI------WEL 123

Query: 121 T-IDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGD 180
              DE T + +I   G  GNPG +TV NWFKI+K    + DYKI +C  VCNFCK++C D
Sbjct: 124 ANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKF---EKDYKIRFCPTVCNFCKVICRD 183

Query: 181 IGAFVEEDGRRLLGFSGQPLLVRFKKA 206
           +G FV+ DG+R L  S  PL V FK+A
Sbjct: 184 VGVFVQ-DGKRRLALSDVPLKVMFKRA 195

[Close]

BLAST of Spo10769.1 vs. TAIR (Arabidopsis)
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 129.0 bits (323), Expect = 3.300e-30
Identity = 75/195 (38.46%), Postives = 112/195 (57.44%), Query Frame = 1


		  
Query: 13  LFLSLSPPTTTAANPAVLDINGHPLRPRSNYILPVVRGRGGGLTMLSKNTTEVCPLYVAQ 72
           L L+L+    + A  AV+DI+G+ +   S Y+LPV+RGRGGGLT+  +   + CP  + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLAGRG-GQPCPYDIVQ 73

Query: 73  ENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFD-ASTICVQSNTWEL-TIDEETGRRYI 132
           E+ EV  G+P+KF +   K   +  S +LN   D  +TIC+QS  W +   D E  + ++
Sbjct: 74  ESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFV 133

Query: 133 GVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFVEEDGRRL 192
             G +    G  ++ ++FKIEK  SG+  YK V+C   C+     C D+G F++E G R 
Sbjct: 134 VAGPKPEGFGQDSLKSFFKIEK--SGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRR 193

Query: 193 LGFSGQPLLVRFKKA 206
           L  S +P LV FKKA
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]

BLAST of Spo10769.1 vs. TAIR (Arabidopsis)
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 84.7 bits (208), Expect = 7.100e-17
Identity = 56/207 (27.05%), Postives = 106/207 (51.21%), Query Frame = 1


		  
Query: 5   FLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPR-SNYILPVVRGRGGGLTMLSKN-- 64
           F++   +    + +       +  VLDI GHP++     YI+P   G GGGL   ++N  
Sbjct: 8   FITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLS 67

Query: 65  TTEVC-PLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDAST-ICVQSNTWEL 124
           T ++C  L + Q +    +GLP+ F  +N K   + +S  LN  FD++  +C  S  W +
Sbjct: 68  TQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI 127

Query: 125 TIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDI 184
               +  + ++ +GG+ G       ++WF+I++ G     YK++YC  + +   + C ++
Sbjct: 128 DHSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDA---YKLMYCP-ISSI--VACINV 187

Query: 185 GAFVEEDG-RRLLGFSGQPLLVRFKKA 206
              +++ G RRL+  + Q  +V+F+KA
Sbjct: 188 SLEIDDHGVRRLVLSTDQSFVVKFQKA 203

The following BLAST results are available for this feature:

BLAST of Spo10769.1 vs. NCBI nr
BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
BLAST of Spo10769.1 vs. TAIR (Arabidopsis)

BLAST of Spo10769.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902194163\|gb\|KNA12470.1\|	4.5e-114	99.5	hypothetical protein SOVF_1257...	[more]
gi\|731344031\|ref\|XP_010683193.1\|	4.2e-88	77.1	PREDICTED: miraculin-like [Bet...	[more]
gi\|731344033\|ref\|XP_010683194.1\|	5.0e-81	75.2	PREDICTED: miraculin-like [Bet...	[more]
gi\|902082350\|gb\|KNA03166.1\|	2.4e-75	66.0	hypothetical protein SOVF_2118...	[more]
gi\|902170260\|gb\|KNA07819.1\|	1.0e-70	62.9	hypothetical protein SOVF_1683...	[more]

BLAST of Spo10769.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QYY3_SPIOL	3.1e-114	99.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8C0T9_BETVU	3.0e-88	77.1	Uncharacterized protein OS=Bet...	[more]
A0A0J8C4X4_BETVU	3.5e-81	75.2	Uncharacterized protein OS=Bet...	[more]
A0A0K9Q9I8_SPIOL	1.7e-75	66.0	Uncharacterized protein OS=Spi...	[more]
A0A0K9QKP3_SPIOL	7.3e-71	62.9	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo10769.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MIRA_SYNDU	4.0e-38	44.2	Miraculin OS=Synsepalum dulcif...	[more]
ASP_THECC	1.6e-26	36.2	21 kDa seed protein OS=Theobro...	[more]
IAAS_ORYSJ	2.1e-23	41.2	Alpha-amylase/subtilisin inhib...	[more]
PIP21_PEA	8.1e-23	37.5	Kunitz-type trypsin inhibitor-...	[more]
PIP22_PEA	4.0e-22	36.0	Kunitz-type trypsin inhibitor-...	[more]

BLAST of Spo10769.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G17860.1	1.0e-39	49.2	Kunitz family trypsin and prot...	[more]
AT1G73260.1	3.3e-30	38.4	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	7.1e-17	27.0	Kunitz family trypsin and prot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 28..57 score: 1.7E-12coord: 66..86 score: 1.7E-12coord: 147..166 score: 1.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..204 score: 4.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..205 score: 1.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 26..204 score: 2.43
None	No IPR available	GENE3D	2.80.10.50		coord: 25..205 score: 2.1
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..205 score: 3.9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0015824	proline transport
biological_process	GO:0006508	proteolysis
biological_process	GO:0009611	response to wounding
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo02312	0.69	Barchart \| Table
Spo19335	0.69	Barchart \| Table
Spo19333	0.66	Barchart \| Table