Spo08305.1 (mRNA) | SpinachBase

Overview


Name	Spo08305.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	MinE protein
Location	SpoScf_00369 : 206790 .. 209714 (+)
Sequence length	1392

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGATAGTGTAAAGTATGTGGTAATGTAACGTAGGTCTAATTAAGCATTTAAGCACCAAGCGCTGTCATAGCTTACCGTTCTTTGTCATAGCAATCGAAGAAGCCAATAACCTTTCTTTGTGCAAGTCTCAGCTTACCGTTCAAGACGCGAGCTATAGAGTGAATGCGCCGGTAAATTCTTCAGGTGAGAGAGAAAAATGGCGATTTCAGGGGATCTGGGAGTATTTGCCACACTCGCACCTCATCGCGCCCGTCATCTTCCTCTTCGAACTTCCTCGCATTCTTCATCTAAGGTCACTTCTACTCCATTTTTACGCTTTTCTGTTGAATTTCTTGCTATTTCCTTTTTTTCAACGTTTATTGGGTACAATTCATATGGGTATATTTTCAATTGTTTCTGAATTCAGCGAATTTTGATGGGATTGTCCGATTTTCAAGGTTTTTTGCGTTTGTGGGTTTTGTTTGATTTTTTTTGGAATGGTTAGGTTTTATTAGGCTTTGATTTGCATACTGCAATGAAATCAGGTTTCTATTTTAGGCATATTTTTGGGCGTTTGTGAAAATTCTGGAAGAAATTTGTTAATATTGTGAGAAGGAGAGGGATGCTCATTTGGGATTTTGGGCCAAAGCCGGGGGTGGAAAGGGAGGATTATGTTTCATGAAGCATTTTCCGGCTCTTCACTGTGGCATATTTTGGGTTTTGTTTGTTTGAGTTTATTTAATTGGAATAATTAGTGACTGATGACTGATCAAGGATTTTAAATTGATGATGGAGTAGTTCTTGGTGAGGGGTTGGGGATTTGGGATGCCTAACGTGAATGTTTCGTTTTCTATTGTTAATGATCAGGGGTTCTGGTACGAATGACCGAAGCGGTTGTGAAAGTTAATTCGTGTTAAATCTTTGTAAAAGCTTCTAGAAAAGGATAGATAAAGTATTCTAGGCTTAGAGCAAGGTTTTAAGTGTAATTCTAGGCTAAAGTTTGTTAGAGAAGGTACAGAGTAAAATATCAGGAAAAAATTCGAGCAAATATTGATAGGGTCAATCTGGGATGGATTGTATTGTCAGATGGCAGTGGACAGCTATTTTGTGAAATCTTGATTATGTGAAATCTTGATTATTGTCAAAGGGTGACAAAACTCAATAGAGGGATAATTTACTTCGTATTATGTTTCCCTAGCTGGGTGGTTTGTCAACAGTCTTAAAATAAATTTGTGACAATGTGACATTAATACTAGAATGAATGTGGGCCTACGTGGCTGTAGAGAAATGGCTTTCGGGTTCTCGGTTTTTTAAGACAAACTTCGGCCTTGATCGTTCTGAGGGTTTTGTAGACTGGTATTTTTCCTGCTGTTTTGTTGACGGAAGATTTTGCTTTTGCTATTCTACCTTAATTATACCCACAATTATAGAAATGCTGGTGTGAGTCTTCCCTTTTTTAGTCCTTTTCTTTCTTTCTCTGCCTAGATTTTGCTTGTTCCAAGTTGAATTTGTCCAGTTAGGAAGAGACTCAAATCCTTGCTCCTCTAATTTACCAGGGAAAATATCCACGGTTAAATGTTACATTACTTGTTATGTTGTTTGTCAGTCACCTTACTCTAGGTACTGACTGTAATGTTAAATTGGCTAGATCATAGTGACGTATGGTCTATCAAGTAATGGTAAAATGCATAATATGAGATGAAATATGTGTTGTTCTGAGGGAGTGGTAATGACACTATATATTAAATTATGAAATCTGACTTTATATGAGTACTGCAATAATGCAAGCTACGTACTACTGCCAGTTACAGCCACTAAATTCATAATATACTTTCCTTGAGTGCAGGTAGACATTTCCTGCTTCCCAAATGGTGGGCTTCGTATTTGTGACTTTACACCGAAAAAGTTGAGCAGTGTGTCTAATGGCTTCAATTTTCGCTGCCAGTCCAATCTGACTTTTGGAGTCAGTGGAGAAGATAAATCATCTGTAAAATCCTTCAGCGAAGATGCTGAGACTTTTTTACTGAATGCTATTAATCTGAGCTTCTTTGAGCGTGTAAACTTAGCATGGAAGATTATTTTCCCCCCACCAAAAACAAGGAGGAGTTCCAATGCAATGATTGCTAAGCAGCGTTTAAAGATGATCTTGTTTGCTGATCGATGTGCTGTTAGTGACGAGGCTAAACGGAAGATAGTGAAAAGCATTGTCAGTGCTTTATCAGACTTTGTGGAGATTGATTCTCAGGATAAGGTGCAGCTCGACGTCTCTACTGATACTGATCTTGGAACTGTATGTTCTGTAACTGTACCTGTAAGGCGAGTGAAACCTGAGTATCAGGAAGACGATGAGTCTGGATCAATAATGGGTGTTGATTATAAAGACAACGGTGAACTATCAGGTTCTGTTGATGTCCAATTTGATTTCTACATCCCAAATGAAAATCAGATGACGTGAGCATACGTGATTTAGGTCTGTAACCTAGTTGCATCAAGTATCATCAGTGATGGCAACTTTTGACCCCAATTTAGATCTCAAGAACATGTGGCTTCTGCATTAGGGGTCGTTACAGATGGAGTTTGTCGCTTCTTGATAACTTGCTTTTCTGTAAATATAAGATGTATTCTCCAAATTGGGTGAAAGATTTATTCTTTCCCTTTATAATCTTAACCTTTTTAGGCTATCATTTTGGAATGAATATGTTTCTAAGGGACCTTAAGTTTTGTATTGCTCTGTTGATGTTCCTTGGTTTATTATGTTTGACAATTTTTCTTGTTGATGGTGTGCAGCACAGAAGATTCTGCATTGTTACTGAAATTAAGGTGATCTGATGGGGGCGGATCACAGGTTTACTGTTAGTAGATGCTATTCGTCCATCAATAAATAAGAAAAAAATATATTATTCCTGCTTACATTTTCCCTATAGCGGAAGGTGTTTCTCCCCAAA

mRNA sequence

ACGATAGTGTAAAGTATGTGGTAATGTAACGTAGGTCTAATTAAGCATTTAAGCACCAAGCGCTGTCATAGCTTACCGTTCTTTGTCATAGCAATCGAAGAAGCCAATAACCTTTCTTTGTGCAAGTCTCAGCTTACCGTTCAAGACGCGAGCTATAGAGTGAATGCGCCGGTAAATTCTTCAGGTGAGAGAGAAAAATGGCGATTTCAGGGGATCTGGGAGTATTTGCCACACTCGCACCTCATCGCGCCCGTCATCTTCCTCTTCGAACTTCCTCGCATTCTTCATCTAAGGTAGACATTTCCTGCTTCCCAAATGGTGGGCTTCGTATTTGTGACTTTACACCGAAAAAGTTGAGCAGTGTGTCTAATGGCTTCAATTTTCGCTGCCAGTCCAATCTGACTTTTGGAGTCAGTGGAGAAGATAAATCATCTGTAAAATCCTTCAGCGAAGATGCTGAGACTTTTTTACTGAATGCTATTAATCTGAGCTTCTTTGAGCGTGTAAACTTAGCATGGAAGATTATTTTCCCCCCACCAAAAACAAGGAGGAGTTCCAATGCAATGATTGCTAAGCAGCGTTTAAAGATGATCTTGTTTGCTGATCGATGTGCTGTTAGTGACGAGGCTAAACGGAAGATAGTGAAAAGCATTGTCAGTGCTTTATCAGACTTTGTGGAGATTGATTCTCAGGATAAGGTGCAGCTCGACGTCTCTACTGATACTGATCTTGGAACTGTATGTTCTGTAACTGTACCTGTAAGGCGAGTGAAACCTGAGTATCAGGAAGACGATGAGTCTGGATCAATAATGGGTGTTGATTATAAAGACAACGGTGAACTATCAGGTTCTGTTGATGTCCAATTTGATTTCTACATCCCAAATGAAAATCAGATGACGTGAGCATACGTGATTTAGGTCTGTAACCTAGTTGCATCAAGTATCATCAGTGATGGCAACTTTTGACCCCAATTTAGATCTCAAGAACATGTGGCTTCTGCATTAGGGGTCGTTACAGATGGAGTTTGTCGCTTCTTGATAACTTGCTTTTCTGTAAATATAAGATGTATTCTCCAAATTGGGTGAAAGATTTATTCTTTCCCTTTATAATCTTAACCTTTTTAGGCTATCATTTTGGAATGAATATGTTTCTAAGGGACCTTAAGTTTTGTATTGCTCTGTTGATGTTCCTTGGTTTATTATGTTTGACAATTTTTCTTGTTGATGGTGTGCAGCACAGAAGATTCTGCATTGTTACTGAAATTAAGGTGATCTGATGGGGGCGGATCACAGGTTTACTGTTAGTAGATGCTATTCGTCCATCAATAAATAAGAAAAAAATATATTATTCCTGCTTACATTTTCCCTATAGCGGAAGGTGTTTCTCCCCAAA

Coding sequence (CDS)

ATGGCGATTTCAGGGGATCTGGGAGTATTTGCCACACTCGCACCTCATCGCGCCCGTCATCTTCCTCTTCGAACTTCCTCGCATTCTTCATCTAAGGTAGACATTTCCTGCTTCCCAAATGGTGGGCTTCGTATTTGTGACTTTACACCGAAAAAGTTGAGCAGTGTGTCTAATGGCTTCAATTTTCGCTGCCAGTCCAATCTGACTTTTGGAGTCAGTGGAGAAGATAAATCATCTGTAAAATCCTTCAGCGAAGATGCTGAGACTTTTTTACTGAATGCTATTAATCTGAGCTTCTTTGAGCGTGTAAACTTAGCATGGAAGATTATTTTCCCCCCACCAAAAACAAGGAGGAGTTCCAATGCAATGATTGCTAAGCAGCGTTTAAAGATGATCTTGTTTGCTGATCGATGTGCTGTTAGTGACGAGGCTAAACGGAAGATAGTGAAAAGCATTGTCAGTGCTTTATCAGACTTTGTGGAGATTGATTCTCAGGATAAGGTGCAGCTCGACGTCTCTACTGATACTGATCTTGGAACTGTATGTTCTGTAACTGTACCTGTAAGGCGAGTGAAACCTGAGTATCAGGAAGACGATGAGTCTGGATCAATAATGGGTGTTGATTATAAAGACAACGGTGAACTATCAGGTTCTGTTGATGTCCAATTTGATTTCTACATCCCAAATGAAAATCAGATGACGTGA

Protein sequence

MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGFNFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSSNAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGTVCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08305	Spo08305	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08305.1	Spo08305.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08305.1.exon.1	Spo08305.1.exon.1	exon
Spo08305.1.exon.2	Spo08305.1.exon.2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08305.1.utr5p.1	Spo08305.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08305.1.CDS.1	Spo08305.1.CDS.1	CDS
Spo08305.1.CDS.2	Spo08305.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08305.1.utr3p.1	Spo08305.1.utr3p.1	three_prime_UTR

Homology

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BLAST of Spo08305.1 vs. NCBI nr
Match: gi|902174285|gb|KNA08401.1| (hypothetical protein SOVF_162970 [Spinacia oleracea])

HSP 1 Score: 461.5 bits (1186), Expect = 9.000e-127
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF
Sbjct: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120
           NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS
Sbjct: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120

Query: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180
           NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT
Sbjct: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180

Query: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT 235
           VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT
Sbjct: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT 234

[Close]

BLAST of Spo08305.1 vs. NCBI nr
Match: gi|731343819|ref|XP_010683082.1| (PREDICTED: cell division topological specificity factor homolog, chloroplastic [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 392.5 bits (1007), Expect = 5.200e-106
Identity = 193/234 (82.48%), Postives = 221/234 (94.44%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MAISGDL VFATLAPHR   LPLRTSSHSSSKVD+S FP+GGL I + +P+KL+SVSNG 
Sbjct: 1   MAISGDLRVFATLAPHRTHQLPLRTSSHSSSKVDVSSFPSGGLSISEISPRKLTSVSNGS 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120
           +FRCQSNL+FGV+G+DK++VKSF++DAETFLL+AINL+FFER+NLAWKI+FPPPKTRRSS
Sbjct: 61  SFRCQSNLSFGVTGDDKTAVKSFNQDAETFLLHAINLNFFERLNLAWKIVFPPPKTRRSS 120

Query: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180
           NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEI+SQ+KVQL+V+TD DLGT
Sbjct: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIESQEKVQLNVATDIDLGT 180

Query: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT 235
           VCSVTVPVRRVKPEYQEDDE+GSIM +DYKDNGE SG+VDV+FDFY+PNEN +T
Sbjct: 181 VCSVTVPVRRVKPEYQEDDETGSIMNIDYKDNGETSGAVDVRFDFYMPNENSLT 234

[Close]

BLAST of Spo08305.1 vs. NCBI nr
Match: gi|661895809|emb|CDP00387.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 277.3 bits (708), Expect = 2.400e-71
Identity = 144/230 (62.61%), Postives = 173/230 (75.22%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MAISGDL V A L PH     PL   +   SKVD   FP GG  + D TPK   +V  G 
Sbjct: 1   MAISGDLRVSAALGPHSIN--PLLRCAFPPSKVDFGSFPGGGSAVFDATPKWSRAVLEGH 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120
           N RC S  + G+  E K S  +  ++ + FL NAIN++FFER+NLAWKI+FP P +RR+S
Sbjct: 61  NTRCHSKRSIGIFSEHKLSSSAIGQELDYFLHNAINMNFFERLNLAWKIVFPSPASRRNS 120

Query: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180
           NA IAK RLKMILF+DRCAVSDEAK+KIV +IV+ALSDFVEI+SQDKVQL VSTD DLGT
Sbjct: 121 NANIAKHRLKMILFSDRCAVSDEAKQKIVSNIVTALSDFVEIESQDKVQLSVSTDPDLGT 180

Query: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNE 231
           V SVTVPVRRV+PEYQE+D +G+I  ++YKDNGE SGSVDV+FDFY+PN+
Sbjct: 181 VYSVTVPVRRVRPEYQEEDPTGTITNIEYKDNGENSGSVDVKFDFYVPNK 228

[Close]

BLAST of Spo08305.1 vs. NCBI nr
Match: gi|738957166|gb|AJA74469.1| (chloroplast MinE [Nicotiana tabacum])

HSP 1 Score: 272.7 bits (696), Expect = 5.900e-70
Identity = 149/231 (64.50%), Postives = 174/231 (75.32%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MA+SGDL V ATL P+     PLRTS    SKVD S F NGG    D  P+     S+G 
Sbjct: 1   MALSGDLRVSATLVPYPKN--PLRTSL-PPSKVDFSAFLNGGSSATDVLPRWSHVASDGH 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPP-PKTRRS 120
           + RCQ+   FG+ GE K S  S S++ +  LLNAIN+SFFER+NLAWKI+FPP P    S
Sbjct: 61  SLRCQARRPFGILGEYKLSTSSISQEFDNLLLNAINMSFFERLNLAWKIMFPPSPSASNS 120

Query: 121 SNAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLG 180
           S A IAKQRLKMILF+DRCAVSDEAK+KIV ++VSALSDFVEI+SQDKVQL VSTD DLG
Sbjct: 121 SAANIAKQRLKMILFSDRCAVSDEAKQKIVSNVVSALSDFVEIESQDKVQLSVSTDPDLG 180

Query: 181 TVCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNE 231
           T+ SVTVPVRRVK EYQE+D +G+I  V+YKD G+ SGSVDV+FDFYIPNE
Sbjct: 181 TIYSVTVPVRRVKSEYQEEDPTGTITNVEYKDTGDSSGSVDVKFDFYIPNE 228

[Close]

BLAST of Spo08305.1 vs. NCBI nr
Match: gi|697165098|ref|XP_009591366.1| (PREDICTED: cell division topological specificity factor homolog, chloroplastic [Nicotiana tomentosiformis])

HSP 1 Score: 272.7 bits (696), Expect = 5.900e-70
Identity = 149/231 (64.50%), Postives = 174/231 (75.32%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MA+SGDL V ATL P+     PLRTS    SKVD S F NGG    D  P+     S+G 
Sbjct: 1   MALSGDLRVSATLVPYPKN--PLRTSL-PPSKVDFSAFLNGGSSATDVLPRWSRVASDGH 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPP-PKTRRS 120
           + RCQ+   FG+ GE K S  S S++ +  LLNAIN+SFFER+NLAWKI+FPP P    S
Sbjct: 61  SLRCQARRPFGILGEYKLSTSSISQEFDNLLLNAINMSFFERLNLAWKIMFPPSPSASNS 120

Query: 121 SNAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLG 180
           S A IAKQRLKMILF+DRCAVSDEAK+KIV ++VSALSDFVEI+SQDKVQL VSTD DLG
Sbjct: 121 SAANIAKQRLKMILFSDRCAVSDEAKQKIVSNVVSALSDFVEIESQDKVQLSVSTDPDLG 180

Query: 181 TVCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNE 231
           T+ SVTVPVRRVK EYQE+D +G+I  V+YKD G+ SGSVDV+FDFYIPNE
Sbjct: 181 TIYSVTVPVRRVKSEYQEEDPTGTITNVEYKDTGDSSGSVDVKFDFYIPNE 228

[Close]

BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QPB5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_162970 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 6.300e-127
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF
Sbjct: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120
           NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS
Sbjct: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120

Query: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180
           NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT
Sbjct: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180

Query: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT 235
           VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT
Sbjct: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT 234

[Close]

BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0H1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g153110 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 3.600e-106
Identity = 193/234 (82.48%), Postives = 221/234 (94.44%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MAISGDL VFATLAPHR   LPLRTSSHSSSKVD+S FP+GGL I + +P+KL+SVSNG 
Sbjct: 1   MAISGDLRVFATLAPHRTHQLPLRTSSHSSSKVDVSSFPSGGLSISEISPRKLTSVSNGS 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120
           +FRCQSNL+FGV+G+DK++VKSF++DAETFLL+AINL+FFER+NLAWKI+FPPPKTRRSS
Sbjct: 61  SFRCQSNLSFGVTGDDKTAVKSFNQDAETFLLHAINLNFFERLNLAWKIVFPPPKTRRSS 120

Query: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180
           NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEI+SQ+KVQL+V+TD DLGT
Sbjct: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIESQEKVQLNVATDIDLGT 180

Query: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNENQMT 235
           VCSVTVPVRRVKPEYQEDDE+GSIM +DYKDNGE SG+VDV+FDFY+PNEN +T
Sbjct: 181 VCSVTVPVRRVKPEYQEDDETGSIMNIDYKDNGETSGAVDVRFDFYMPNENSLT 234

[Close]

BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
Match: A0A068TWG2_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00032313001 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.700e-71
Identity = 144/230 (62.61%), Postives = 173/230 (75.22%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MAISGDL V A L PH     PL   +   SKVD   FP GG  + D TPK   +V  G 
Sbjct: 1   MAISGDLRVSAALGPHSIN--PLLRCAFPPSKVDFGSFPGGGSAVFDATPKWSRAVLEGH 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSS 120
           N RC S  + G+  E K S  +  ++ + FL NAIN++FFER+NLAWKI+FP P +RR+S
Sbjct: 61  NTRCHSKRSIGIFSEHKLSSSAIGQELDYFLHNAINMNFFERLNLAWKIVFPSPASRRNS 120

Query: 121 NAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGT 180
           NA IAK RLKMILF+DRCAVSDEAK+KIV +IV+ALSDFVEI+SQDKVQL VSTD DLGT
Sbjct: 121 NANIAKHRLKMILFSDRCAVSDEAKQKIVSNIVTALSDFVEIESQDKVQLSVSTDPDLGT 180

Query: 181 VCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNE 231
           V SVTVPVRRV+PEYQE+D +G+I  ++YKDNGE SGSVDV+FDFY+PN+
Sbjct: 181 VYSVTVPVRRVRPEYQEEDPTGTITNIEYKDNGENSGSVDVKFDFYVPNK 228

[Close]

BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
Match: A0A0A7U556_TOBAC (Chloroplast MinE OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 4.100e-70
Identity = 149/231 (64.50%), Postives = 174/231 (75.32%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGF 60
           MA+SGDL V ATL P+     PLRTS    SKVD S F NGG    D  P+     S+G 
Sbjct: 1   MALSGDLRVSATLVPYPKN--PLRTSL-PPSKVDFSAFLNGGSSATDVLPRWSHVASDGH 60

Query: 61  NFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPP-PKTRRS 120
           + RCQ+   FG+ GE K S  S S++ +  LLNAIN+SFFER+NLAWKI+FPP P    S
Sbjct: 61  SLRCQARRPFGILGEYKLSTSSISQEFDNLLLNAINMSFFERLNLAWKIMFPPSPSASNS 120

Query: 121 SNAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLG 180
           S A IAKQRLKMILF+DRCAVSDEAK+KIV ++VSALSDFVEI+SQDKVQL VSTD DLG
Sbjct: 121 SAANIAKQRLKMILFSDRCAVSDEAKQKIVSNVVSALSDFVEIESQDKVQLSVSTDPDLG 180

Query: 181 TVCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNE 231
           T+ SVTVPVRRVK EYQE+D +G+I  V+YKD G+ SGSVDV+FDFYIPNE
Sbjct: 181 TIYSVTVPVRRVKSEYQEEDPTGTITNVEYKDTGDSSGSVDVKFDFYIPNE 228

[Close]

BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
Match: A0A022QMI6_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a013165mg PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.500e-67
Identity = 140/231 (60.61%), Postives = 176/231 (76.19%), Query Frame = 1

		  
Query: 1   MAISGDLGVFATLAPHRARHLPLRTSSH-SSSKVDISCFPNGGLRICDFTPKKLSSVSNG 60
           MAISGDL V A L+ ++    PLRT SH   SKVD + F NG   + +  P+   +V + 
Sbjct: 1   MAISGDLRVSAALSSYKFN--PLRTRSHFPPSKVDCNVFTNGASSVFESIPRCSRTVLDP 60

Query: 61  FNFRCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRS 120
            + RC S    G+ G+ K S    S++ E FLLNAIN++FF+RV+LAWKIIFP P +RR+
Sbjct: 61  HSTRCHSKRPIGIFGDYKVSENPISQEVENFLLNAINMNFFDRVSLAWKIIFPSPTSRRN 120

Query: 121 SNAMIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLG 180
           SNA IAKQRL+MILF+DRCAVS+EAK+KIV ++VSALSDFVEI+SQDKVQL VSTD DLG
Sbjct: 121 SNANIAKQRLRMILFSDRCAVSEEAKQKIVSNVVSALSDFVEIESQDKVQLSVSTDPDLG 180

Query: 181 TVCSVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIPNE 231
           T+ SVTVPVRRV+ EYQ DDE+G+I  ++YKD GE SGSVDV+FDFY+P+E
Sbjct: 181 TIYSVTVPVRRVRTEYQLDDETGAITNIEYKDTGESSGSVDVKFDFYVPSE 229

[Close]

BLAST of Spo08305.1 vs. ExPASy Swiss-Prot
Match: MINE1_ARATH (Cell division topological specificity factor homolog, chloroplastic OS=Arabidopsis thaliana GN=MINE1 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 4.900e-56
Identity = 124/226 (54.87%), Postives = 159/226 (70.35%), Query Frame = 1

		  
Query: 4   SGDLGVFATL-APHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGFNF 63
           SG L + ATL +P+   H    +   SSSKVD + F + G+   +          +  N 
Sbjct: 5   SGTLRISATLVSPYHHHHRNRLSLPSSSSKVDFTGFISNGVNSLETQKCTPGLAISRENT 64

Query: 64  RCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSSNA 123
           R Q  +    +G+ + S     ++ E+FL NAIN+ FF+R+NLAWKIIFP   +RRSSNA
Sbjct: 65  RGQVKVLARNTGDYELSPSPAEQEIESFLYNAINMGFFDRLNLAWKIIFPSHASRRSSNA 124

Query: 124 MIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGTVC 183
            IAKQRLKMILF+DRC VSDEAKRKIV +I+ ALSDFVEI+S++KVQL+VSTD DLGT+ 
Sbjct: 125 RIAKQRLKMILFSDRCDVSDEAKRKIVNNIIHALSDFVEIESEEKVQLNVSTDGDLGTIY 184

Query: 184 SVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIP 229
           SVTVPVRRVKPEYQ+ DE+G+I  V+YKD  +  GSVDV+FDFY+P
Sbjct: 185 SVTVPVRRVKPEYQDVDEAGTITNVEYKDTRD--GSVDVRFDFYVP 228

[Close]

BLAST of Spo08305.1 vs. TAIR (Arabidopsis)
Match: AT1G69390.1 (homologue of bacterial MinE 1)

HSP 1 Score: 219.2 bits (557), Expect = 2.800e-57
Identity = 124/226 (54.87%), Postives = 159/226 (70.35%), Query Frame = 1

		  
Query: 4   SGDLGVFATL-APHRARHLPLRTSSHSSSKVDISCFPNGGLRICDFTPKKLSSVSNGFNF 63
           SG L + ATL +P+   H    +   SSSKVD + F + G+   +          +  N 
Sbjct: 5   SGTLRISATLVSPYHHHHRNRLSLPSSSSKVDFTGFISNGVNSLETQKCTPGLAISRENT 64

Query: 64  RCQSNLTFGVSGEDKSSVKSFSEDAETFLLNAINLSFFERVNLAWKIIFPPPKTRRSSNA 123
           R Q  +    +G+ + S     ++ E+FL NAIN+ FF+R+NLAWKIIFP   +RRSSNA
Sbjct: 65  RGQVKVLARNTGDYELSPSPAEQEIESFLYNAINMGFFDRLNLAWKIIFPSHASRRSSNA 124

Query: 124 MIAKQRLKMILFADRCAVSDEAKRKIVKSIVSALSDFVEIDSQDKVQLDVSTDTDLGTVC 183
            IAKQRLKMILF+DRC VSDEAKRKIV +I+ ALSDFVEI+S++KVQL+VSTD DLGT+ 
Sbjct: 125 RIAKQRLKMILFSDRCDVSDEAKRKIVNNIIHALSDFVEIESEEKVQLNVSTDGDLGTIY 184

Query: 184 SVTVPVRRVKPEYQEDDESGSIMGVDYKDNGELSGSVDVQFDFYIP 229
           SVTVPVRRVKPEYQ+ DE+G+I  V+YKD  +  GSVDV+FDFY+P
Sbjct: 185 SVTVPVRRVKPEYQDVDEAGTITNVEYKDTRD--GSVDVRFDFYVP 228

The following BLAST results are available for this feature:

BLAST of Spo08305.1 vs. NCBI nr
BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
BLAST of Spo08305.1 vs. ExPASy Swiss-Prot
BLAST of Spo08305.1 vs. TAIR (Arabidopsis)

BLAST of Spo08305.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902174285\|gb\|KNA08401.1\|	9.0e-127	100.	hypothetical protein SOVF_1629...	[more]
gi\|731343819\|ref\|XP_010683082.1\|	5.2e-106	82.4	PREDICTED: cell division topol...	[more]
gi\|661895809\|emb\|CDP00387.1\|	2.4e-71	62.6	unnamed protein product [Coffe...	[more]
gi\|738957166\|gb\|AJA74469.1\|	5.9e-70	64.5	chloroplast MinE [Nicotiana ta...	[more]
gi\|697165098\|ref\|XP_009591366.1\|	5.9e-70	64.5	PREDICTED: cell division topol...	[more]

BLAST of Spo08305.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
A0A0K9QPB5_SPIOL	6.3e-127	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8C0H1_BETVU	3.6e-106	82.4	Uncharacterized protein OS=Bet...	[more]
A0A068TWG2_COFCA	1.7e-71	62.6	Uncharacterized protein OS=Cof...	[more]
A0A0A7U556_TOBAC	4.1e-70	64.5	Chloroplast MinE OS=Nicotiana ...	[more]
A0A022QMI6_ERYGU	1.5e-67	60.6	Uncharacterized protein OS=Ery...	[more]

BLAST of Spo08305.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1

Position :

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Match Name	E-value	Identity	Description
MINE1_ARATH	4.9e-56	54.8	Cell division topological spec...	[more]

BLAST of Spo08305.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1

Position :

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Match Name	E-value	Identity	Description
AT1G69390.1	2.8e-57	54.8	homologue of bacterial MinE 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005527	Septum formation topological specificity factor MinE	PFAM	PF03776	MinE	coord: 123..189 score: 3.9
None	No IPR available	PANTHER	PTHR33404	FAMILY NOT NAMED	coord: 22..231 score: 5.2
None	No IPR available	PANTHER	PTHR33404:SF2	CELL DIVISION TOPOLOGICAL SPECIFICITY FACTOR HOMOLOG, CHLOROPLASTIC	coord: 22..231 score: 5.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0032955	regulation of division septum assembly
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0005515	protein binding