Spo07639 (gene) | SpinachBase

Overview


Name	Spo07639
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	High affinity nitrate transporter
Location	chr1 : 43736484 .. 43738675 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAATAGAGTATCCTTCAAAGTGGGGTCTCATCAAGTACACAATATAAATAGCAATAGCCAACTACGGAAGAATCATAAACCAGAATCACAAAAACAAAAAAAAAAGTATCAATTTTTTTTGGTGTGGAAGTTTTTAGCAATTTTTGTTTCTTCAAAATCTTGAAATTGGAGCTAGAAGCCTAAAAACAAAAAGAAAACAGATTAGTGATGGCGGTTCGAGGTTTAATAATCTTTGTTGCATTACTCTCAAGCTTGGCGGCATCTTGCTATGGAGTCATGTTCCATGAGCTTGCTCATAGTCTCACCGTCTCTTCTACTCCCTCTGGCCAAGGTAACATTACTTAATTATCCTTTTCTCAATTTGATTAAAAAGTCAACATGTATCAAACTCAAACTCAAACTCAATATCATAGGTTTTTTTTTTATTCAAAATGAATATTTATACATTCGAAATGAATTCATTATATTATCGATGGGAAGGGGTCTCAATTCATCTCAAATAAATTGTAAATTCTGGCCAGAATACATAAATACAGGGATTGTTGATTGTCTATTTCATTTACTCCTGTATACAAGGAATAATCTGAATAAGATATTGTTGTGGGTAGGATAGAATTTAATCTCATCACGTGGTTGAGTAATTTCAACAAAAGACGCACTTTTGTACGTAGATTTGATCGAGTTAGTAGAGTTGACTTCCTGGATAGATTTTTTTTTCTTTTTTTTTCTACAAATGAGCCAAGGAGGCATTATAAATTACAAATATGTGACCCTCAATTTCTGGCACTCCTGCATAGATTAAGAGAGAAAGGAGTAATTACATGTCTTAATCTCAAATGAAAACCTAATGAGTTTCCCTAGTGTGATATCTTAAAACACCCTTTTGAATATTTTTTTCACAAATTCTTATTTAAAGGTGGTGTACAATAGATATTGCATATCGAAGTAAAAGTGAGAGGTAAAATATTTAAAAGTCATCCGATATAGCGTAAAAATTATCTATTTTTTAGTGATAAAATTTTTCAATTTAATAAAAATTATCCCTTCAAATCACTAATATGGAGCAATTAATGTATAAACATAACTCTGTAAGCTTTTAAAATGTTTATTCATCAAAACAAAAATTATAATTATTAAGTTAGTCAAAACATGTTAAAGTTACCTAAATGTACAACAAATTTATTGTATTTCTTATGCATGCAAGAATTTTTTGTCAATATTTTCTAATTCAGATGCATTTAGAAACTCCCAGACGGAGTAGTAATTTAGGGAGAAACTCCAAATATATAGAGGTCATGTATCGATAAATGGTCCCTAAAAGGACACGAAAAATAAGCATATCACCATTATAAATTCGTAGAGACTACTTTTTAATGTTTGTGTTTATGACGTAATAATACTTTGATGAAACAGCAAATGTAAAGGCAGGGAAAGATCAAATAACAGTGACATGGGCCCTAAACAGAAGCATATCCGGTGTCGATACATCAATCTACAGGGAGGTAGAAGCAAAGTTATGCTACCATTTAGAAAGCCAAAAAGACAGGCCATGGAGGAAAACTGAAGAAGAAATGGCAAGAGACAAAACCTGTCAATTCGCAATCGTTAAAAGGCCGTACACTTCATCAAGTGACTCAGTAACCTACACTATTAAGAAGGATGTTCCAACTGCACATTACTTTATCCGAGTCTACGTTCGTGACGGTCCTGGTGGAAAACTCATCGCTTACGGTCAAACCACCGGTTTAGACTTGTTTGTCGCCGGAATCAGTGGCCGTAGCGCCTCCATTGATATTGCTGCTTCTATATTCTCTGCTTTCTCTGTTATTTCTCTTGGCTTTTTCTTCTACTTGGAGAAGAAGAAGTCTAAGAGGGCAACTTAGATTATATTTTTCCAGCCAACGGAAAAAAAAAACCTAAGGGTTAAATTTCTTTTGCATTACTTTACTTTGTGAGGAAATTATGTGTTATTTTCTTATGTATTTACTTGATGAAATTTGTGTAATAGGCTTTTGGCTTCAATTACACCATAAATCATCTTTTAACCAAAATATATGTTGTAATCTTTTGTTGTTTGGCAATTAGTCAAATGTTAGAATTTTGGAGAAATGATTTGGTTAAAAAGCTTTTAAGTTTTAACCCAATAATTATTTTAATACGTTCCTTTGAGATGTCCATTCTATATTCTT

mRNA sequence

AATTAATAGAGTATCCTTCAAAGTGGGGTCTCATCAAGTACACAATATAAATAGCAATAGCCAACTACGGAAGAATCATAAACCAGAATCACAAAAACAAAAAAAAAAGTATCAATTTTTTTTGGTGTGGAAGTTTTTAGCAATTTTTGTTTCTTCAAAATCTTGAAATTGGAGCTAGAAGCCTAAAAACAAAAAGAAAACAGATTAGTGATGGCGGTTCGAGGTTTAATAATCTTTGTTGCATTACTCTCAAGCTTGGCGGCATCTTGCTATGGAGTCATGTTCCATGAGCTTGCTCATAGTCTCACCGTCTCTTCTACTCCCTCTGGCCAAGCAAATGTAAAGGCAGGGAAAGATCAAATAACAGTGACATGGGCCCTAAACAGAAGCATATCCGGTGTCGATACATCAATCTACAGGGAGGTAGAAGCAAAGTTATGCTACCATTTAGAAAGCCAAAAAGACAGGCCATGGAGGAAAACTGAAGAAGAAATGGCAAGAGACAAAACCTGTCAATTCGCAATCGTTAAAAGGCCGTACACTTCATCAAGTGACTCAGTAACCTACACTATTAAGAAGGATGTTCCAACTGCACATTACTTTATCCGAGTCTACGTTCGTGACGGTCCTGGTGGAAAACTCATCGCTTACGGTCAAACCACCGGTTTAGACTTGTTTGTCGCCGGAATCAGTGGCCGTAGCGCCTCCATTGATATTGCTGCTTCTATATTCTCTGCTTTCTCTGTTATTTCTCTTGGCTTTTTCTTCTACTTGGAGAAGAAGAAGTCTAAGAGGGCAACTTAGATTATATTTTTCCAGCCAACGGAAAAAAAAAACCTAAGGGTTAAATTTCTTTTGCATTACTTTACTTTGTGAGGAAATTATGTGTTATTTTCTTATGTATTTACTTGATGAAATTTGTGTAATAGGCTTTTGGCTTCAATTACACCATAAATCATCTTTTAACCAAAATATATGTTGTAATCTTTTGTTGTTTGGCAATTAGTCAAATGTTAGAATTTTGGAGAAATGATTTGGTTAAAAAGCTTTTAAGTTTTAACCCAATAATTATTTTAATACGTTCCTTTGAGATGTCCATTCTATATTCTT

Coding sequence (CDS)

ATGGCGGTTCGAGGTTTAATAATCTTTGTTGCATTACTCTCAAGCTTGGCGGCATCTTGCTATGGAGTCATGTTCCATGAGCTTGCTCATAGTCTCACCGTCTCTTCTACTCCCTCTGGCCAAGCAAATGTAAAGGCAGGGAAAGATCAAATAACAGTGACATGGGCCCTAAACAGAAGCATATCCGGTGTCGATACATCAATCTACAGGGAGGTAGAAGCAAAGTTATGCTACCATTTAGAAAGCCAAAAAGACAGGCCATGGAGGAAAACTGAAGAAGAAATGGCAAGAGACAAAACCTGTCAATTCGCAATCGTTAAAAGGCCGTACACTTCATCAAGTGACTCAGTAACCTACACTATTAAGAAGGATGTTCCAACTGCACATTACTTTATCCGAGTCTACGTTCGTGACGGTCCTGGTGGAAAACTCATCGCTTACGGTCAAACCACCGGTTTAGACTTGTTTGTCGCCGGAATCAGTGGCCGTAGCGCCTCCATTGATATTGCTGCTTCTATATTCTCTGCTTTCTCTGTTATTTCTCTTGGCTTTTTCTTCTACTTGGAGAAGAAGAAGTCTAAGAGGGCAACTTAG

Protein sequence

MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRSISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYTIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVISLGFFFYLEKKKSKRAT

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo07639.1	Spo07639.1	mRNA

Homology

[Close]

BLAST of Spo07639.1 vs. NCBI nr
Match: gi|902212412|gb|KNA16950.1| (hypothetical protein SOVF_084270 [Spinacia oleracea])

HSP 1 Score: 380.9 bits (977), Expect = 1.300e-102
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS
Sbjct: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
           ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT
Sbjct: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVI 180
           IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVI
Sbjct: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVI 180

Query: 181 SLGFFFYLEKKKSKRAT 198
           SLGFFFYLEKKKSKRAT
Sbjct: 181 SLGFFFYLEKKKSKRAT 197

[Close]

BLAST of Spo07639.1 vs. NCBI nr
Match: gi|731376224|ref|XP_010667500.1| (PREDICTED: high-affinity nitrate transporter 3.2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 258.5 bits (659), Expect = 9.800e-66
Identity = 127/198 (64.14%), Postives = 167/198 (84.34%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGV-MFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNR 60
           M VRGLIIF A+L SL A+CY   +F +L ++LTVS++PSG+ +++AG DQITV+W LNR
Sbjct: 1   MGVRGLIIFAAVLCSLVATCYSKGVFADLRNTLTVSASPSGRVDLRAGIDQITVSWRLNR 60

Query: 61  SISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTY 120
           +IS  D++ Y +V+ KLCYHLESQKDRPWR+TEE+++RDKTCQF++VK+ Y SS+DSVTY
Sbjct: 61  NISNTDSATYSKVDVKLCYHLESQKDRPWRRTEEDLSRDKTCQFSMVKKDYNSSTDSVTY 120

Query: 121 TIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSV 180
           T+KK++PTAHYF+RVYVR+    + IAYGQT GLDL + GISGRS SID+AAS+FS FSV
Sbjct: 121 TVKKNIPTAHYFVRVYVRNA-DNREIAYGQTNGLDLNIKGISGRSTSIDVAASVFSGFSV 180

Query: 181 ISLGFFFYLEKKKSKRAT 198
           +SL FFF+LEK+K+K+ T
Sbjct: 181 LSLAFFFFLEKRKAKKLT 197

[Close]

BLAST of Spo07639.1 vs. NCBI nr
Match: gi|147825149|emb|CAN71077.1| (hypothetical protein VITISV_021663 [Vitis vinifera])

HSP 1 Score: 169.1 bits (427), Expect = 7.800e-39
Identity = 99/201 (49.25%), Postives = 131/201 (65.17%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MA R  ++   LLS L   C G  F  L  +L V+++P     +K+G+D+ITVTW LN+S
Sbjct: 1   MATRAFLLTSLLLSCLLQICSGAYFSSLQRTLIVTASPKAGEVLKSGEDKITVTWGLNQS 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
                 S Y+ V+ KLCY   SQ DR WRKT + + +DKTCQ  IV +PY +S++SV +T
Sbjct: 61  YPAGTDSAYKTVKVKLCYAPISQVDRAWRKTVDHLTKDKTCQHKIVSQPYKASNNSVEWT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASIDIAASIFS 180
           I+KDVPTA YFIR Y  D    + +AYGQTT      +LF +  I+GR AS+DIAA  FS
Sbjct: 121 IEKDVPTATYFIRAYAYDAEDQE-VAYGQTTDAHKTTNLFGIEAITGRHASLDIAAVCFS 180

Query: 181 AFSVISLGFFFYLEKKKSKRA 197
           AFSVISL  FF++EK+KS+ A
Sbjct: 181 AFSVISLCGFFWVEKRKSRVA 200

[Close]

BLAST of Spo07639.1 vs. NCBI nr
Match: gi|225456868|ref|XP_002279861.1| (PREDICTED: high-affinity nitrate transporter 3.1 [Vitis vinifera])

HSP 1 Score: 168.7 bits (426), Expect = 1.000e-38
Identity = 98/201 (48.76%), Postives = 131/201 (65.17%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MA R  ++   LLS L   C G  F  L  +L V+++P     +K+G+D+ITVTW LN+S
Sbjct: 1   MATRAFLLTSLLLSCLLQICSGAYFSSLQRTLIVTASPKAGEVLKSGEDKITVTWGLNQS 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
                 S Y+ V+ KLCY   SQ DR WRKT + + +DKTCQ  IV +PY +S++SV +T
Sbjct: 61  YPAGTDSAYKTVKVKLCYAPISQVDRAWRKTVDHLTKDKTCQHKIVSQPYKASNNSVEWT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASIDIAASIFS 180
           I+KDVPTA YFIR Y  D    + +AYGQTT      +LF +  I+GR AS+DIAA  FS
Sbjct: 121 IEKDVPTATYFIRAYAYDAEDQE-VAYGQTTDAHKTTNLFGIEAITGRHASLDIAAVCFS 180

Query: 181 AFSVISLGFFFYLEKKKSKRA 197
           AFSV+SL  FF++EK+KS+ A
Sbjct: 181 AFSVVSLCGFFWVEKRKSRVA 200

[Close]

BLAST of Spo07639.1 vs. NCBI nr
Match: gi|661883623|emb|CDP12708.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 168.7 bits (426), Expect = 1.000e-38
Identity = 94/199 (47.24%), Postives = 137/199 (68.84%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MA  G ++ + +LS LAAS  GV F  L  +L V ++P+    +KAG+D+ITVTW+ N +
Sbjct: 1   MAAGGFLVALLVLSCLAASSNGVTFSSLQRTLIVRASPTQGQVLKAGEDKITVTWSYNNT 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
           ++    S Y+ ++ KLCY   SQ DR WRKT +++A+DKTCQF IV +PY++S+ ++T+T
Sbjct: 61  LAPGTDSTYKTIKVKLCYAPISQVDRAWRKTVDDLAKDKTCQFKIVAKPYSASNSTITWT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASIDIAASIFS 180
           I++D PTA YF+R Y  +    + +A+GQTT      +LF V  ISGR AS+DIAA  FS
Sbjct: 121 IERDTPTATYFLRAYAFNS-AEEEVAFGQTTDAHKTTNLFQVEAISGRHASLDIAAICFS 180

Query: 181 AFSVISLGFFFYLEKKKSK 195
           +FSV+SL  FF++EK+K K
Sbjct: 181 SFSVLSLFGFFFIEKRKGK 198

[Close]

BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RBT8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_084270 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 9.100e-103
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS
Sbjct: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
           ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT
Sbjct: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVI 180
           IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVI
Sbjct: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSVI 180

Query: 181 SLGFFFYLEKKKSKRAT 198
           SLGFFFYLEKKKSKRAT
Sbjct: 181 SLGFFFYLEKKKSKRAT 197

[Close]

BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B2J0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_009170 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 6.800e-66
Identity = 127/198 (64.14%), Postives = 167/198 (84.34%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGV-MFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNR 60
           M VRGLIIF A+L SL A+CY   +F +L ++LTVS++PSG+ +++AG DQITV+W LNR
Sbjct: 1   MGVRGLIIFAAVLCSLVATCYSKGVFADLRNTLTVSASPSGRVDLRAGIDQITVSWRLNR 60

Query: 61  SISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTY 120
           +IS  D++ Y +V+ KLCYHLESQKDRPWR+TEE+++RDKTCQF++VK+ Y SS+DSVTY
Sbjct: 61  NISNTDSATYSKVDVKLCYHLESQKDRPWRRTEEDLSRDKTCQFSMVKKDYNSSTDSVTY 120

Query: 121 TIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLFVAGISGRSASIDIAASIFSAFSV 180
           T+KK++PTAHYF+RVYVR+    + IAYGQT GLDL + GISGRS SID+AAS+FS FSV
Sbjct: 121 TVKKNIPTAHYFVRVYVRNA-DNREIAYGQTNGLDLNIKGISGRSTSIDVAASVFSGFSV 180

Query: 181 ISLGFFFYLEKKKSKRAT 198
           +SL FFF+LEK+K+K+ T
Sbjct: 181 LSLAFFFFLEKRKAKKLT 197

[Close]

BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
Match: A5CB21_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021663 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 5.400e-39
Identity = 99/201 (49.25%), Postives = 131/201 (65.17%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MA R  ++   LLS L   C G  F  L  +L V+++P     +K+G+D+ITVTW LN+S
Sbjct: 1   MATRAFLLTSLLLSCLLQICSGAYFSSLQRTLIVTASPKAGEVLKSGEDKITVTWGLNQS 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
                 S Y+ V+ KLCY   SQ DR WRKT + + +DKTCQ  IV +PY +S++SV +T
Sbjct: 61  YPAGTDSAYKTVKVKLCYAPISQVDRAWRKTVDHLTKDKTCQHKIVSQPYKASNNSVEWT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASIDIAASIFS 180
           I+KDVPTA YFIR Y  D    + +AYGQTT      +LF +  I+GR AS+DIAA  FS
Sbjct: 121 IEKDVPTATYFIRAYAYDAEDQE-VAYGQTTDAHKTTNLFGIEAITGRHASLDIAAVCFS 180

Query: 181 AFSVISLGFFFYLEKKKSKRA 197
           AFSVISL  FF++EK+KS+ A
Sbjct: 181 AFSVISLCGFFWVEKRKSRVA 200

[Close]

BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
Match: A0A068UYM9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00037307001 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.100e-39
Identity = 94/199 (47.24%), Postives = 137/199 (68.84%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRS 60
           MA  G ++ + +LS LAAS  GV F  L  +L V ++P+    +KAG+D+ITVTW+ N +
Sbjct: 1   MAAGGFLVALLVLSCLAASSNGVTFSSLQRTLIVRASPTQGQVLKAGEDKITVTWSYNNT 60

Query: 61  ISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYT 120
           ++    S Y+ ++ KLCY   SQ DR WRKT +++A+DKTCQF IV +PY++S+ ++T+T
Sbjct: 61  LAPGTDSTYKTIKVKLCYAPISQVDRAWRKTVDDLAKDKTCQFKIVAKPYSASNSTITWT 120

Query: 121 IKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASIDIAASIFS 180
           I++D PTA YF+R Y  +    + +A+GQTT      +LF V  ISGR AS+DIAA  FS
Sbjct: 121 IERDTPTATYFLRAYAFNS-AEEEVAFGQTTDAHKTTNLFQVEAISGRHASLDIAAICFS 180

Query: 181 AFSVISLGFFFYLEKKKSK 195
           +FSV+SL  FF++EK+K K
Sbjct: 181 SFSVLSLFGFFFIEKRKGK 198

[Close]

BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
Match: A0A124SGG2_CYNCS (Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_015380 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.100e-39
Identity = 92/188 (48.94%), Postives = 128/188 (68.09%), Query Frame = 1


		  
Query: 12  LLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRSISGVDTSIYRE 71
           L S  AA+C GV F  L  +L V+++ +    +KAG++QITVTWA N++      S YR 
Sbjct: 13  LFSCFAATCTGVTFSSLQKTLVVTASSTQDQVLKAGEEQITVTWAFNQTFPAGTDSAYRT 72

Query: 72  VEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYTIKKDVPTAHYF 131
           V+ KLCY   SQKDR WRKTE EM +DKTCQ  IV RPY++S++S ++TI +D+P+A YF
Sbjct: 73  VKVKLCYAPISQKDRSWRKTEXEMKKDKTCQHKIVSRPYSASNNSFSWTIMRDIPSATYF 132

Query: 132 IRVYVRDGPGGKLIAYGQTTGLD----LF-VAGISGRSASIDIAASIFSAFSVISLGFFF 191
           +R YV +    + +A+GQ T ++    LF V  I+GR AS+DIA+  FSAFS+++L  FF
Sbjct: 133 VRAYVFNSQDQE-VAFGQNTNVERATNLFEVQAITGRHASLDIASICFSAFSIVALAGFF 192

Query: 192 YLEKKKSK 195
           Y+EK K K
Sbjct: 193 YMEKSKGK 199

[Close]

BLAST of Spo07639.1 vs. ExPASy Swiss-Prot
Match: NRT32_ARATH (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.700e-31
Identity = 84/205 (40.98%), Postives = 126/205 (61.46%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGV----MFHELAHSLTVSSTPSGQANV-KAGKDQITVTW 60
           MA+  L+    L+ SL  S  G     +F +L +S+ V++ P   + V +AGKD +T+TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  ALNRSISGVDT-SIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSS 120
            L  S + VDT + ++ ++ KLCY   SQ DRPWRKT+ ++ +D++C   IV + Y  + 
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYDKTP 120

Query: 121 DSVTYTIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASIDI 180
            S+ +TI  D+PT  YF+R Y  DG G + +AYGQ+T      +LF V  ISG    +DI
Sbjct: 121 QSLDWTIGLDIPTGTYFVRAYGIDGDGHE-VAYGQSTDEGRTTNLFSVHAISGHHVGLDI 180

Query: 181 AASIFSAFSVISLGFFFYLEKKKSK 195
           A++ FS FSV+SL  FF +EK+K+K
Sbjct: 181 ASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]

BLAST of Spo07639.1 vs. ExPASy Swiss-Prot
Match: NAR21_ORYSJ (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.000e-30
Identity = 71/183 (38.80%), Postives = 111/183 (60.66%), Query Frame = 1


		  
Query: 14  SSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRSISGVDTSIYREVE 73
           ++ AA+   V   +L  +L V  +P     V AG++ +TVTW+LN S      + ++ V+
Sbjct: 25  AAAAAAKTQVFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVK 84

Query: 74  AKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYTIKKDVPTAHYFIR 133
            KLCY   S+ DR WRK  +++ +DK CQF +  +PY + +    Y + +D+PTA YF+R
Sbjct: 85  VKLCYAPASRTDRGWRKASDDLHKDKACQFKVTVQPYAAGAGRFDYVVARDIPTASYFVR 144

Query: 134 VYVRDGPGGKLIAYGQTTGLDLF-VAGISGRSASIDIAASIFSAFSVISLGFFFYLEKKK 193
            Y  D  G + +AYGQ++    F VAGI+G  AS+ +AA +FS FS+ +L FFF +EK+K
Sbjct: 145 AYAVDASGTE-VAYGQSSPDAAFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRK 204

Query: 194 SKR 196
             +
Sbjct: 205 KDK 206

[Close]

BLAST of Spo07639.1 vs. ExPASy Swiss-Prot
Match: NRT31_ARATH (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.000e-30
Identity = 85/207 (41.06%), Postives = 127/207 (61.35%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGV----MFHELAH-SLTVSSTPSGQAN---VKAGKDQIT 60
           MA++ ++    L+ SL  S +G     +F EL   +L V++ PS +     + AGKD + 
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWALNRSISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTS 120
           +TW L+ SI     + ++ ++ KLCY   SQ DRPWRKT +E+ +DKTC   I+ +PY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYDK 120

Query: 121 SSDSVTYTIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASI 180
           +  S T+T+++D+PT  YF+R Y  D  G + +AYGQ+T      +LF V  ISGR AS+
Sbjct: 121 TLQSTTWTLERDIPTGTYFVRAYAVDAIGHE-VAYGQSTDDAKKTNLFSVQAISGRHASL 180

Query: 181 DIAASIFSAFSVISLGFFFYLEKKKSK 195
           DIA+  FS FSV++L  FF  EK+K+K
Sbjct: 181 DIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Spo07639.1 vs. ExPASy Swiss-Prot
Match: NAR22_ORYSJ (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.100e-30
Identity = 79/195 (40.51%), Postives = 115/195 (58.97%), Query Frame = 1


		  
Query: 6   LIIFVALLSSLAASCYGVMFHELAHSLTVSSTPSGQANVKAGKDQITVTWALNRSIS--- 65
           L++  A   + AA+  G     LA +L V ++P     + AG+D ITVTW+LN + +   
Sbjct: 19  LVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTWSLNATAAAAA 78

Query: 66  -GVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTSSSDSVTYTI 125
            G D   Y+ V+  LCY   SQ  R WRK  +++++DK CQF I ++PY   +    YT+
Sbjct: 79  AGADAG-YKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPY-DGAGKFEYTV 138

Query: 126 KKDVPTAHYFIRVYVRDGPGGKLIAYGQTTGLDLF-VAGISGRSASIDIAASIFSAFSVI 185
            +DVPTA Y++R Y  D  G + +AYG+T     F VAGI+G +ASI++AA + SAFSV 
Sbjct: 139 ARDVPTASYYVRAYALDASGAR-VAYGETAPSASFAVAGITGVTASIEVAAGVLSAFSVA 198

Query: 186 SLGFFFYLEKKKSKR 196
           +L  F  LE KK  +
Sbjct: 199 ALAVFLVLENKKKNK 210

[Close]

BLAST of Spo07639.1 vs. TAIR (Arabidopsis)
Match: AT5G50200.3 (nitrate transmembrane transporters)

HSP 1 Score: 134.8 bits (338), Expect = 5.700e-32
Identity = 85/207 (41.06%), Postives = 127/207 (61.35%), Query Frame = 1


		  
Query: 1   MAVRGLIIFVALLSSLAASCYGV----MFHELAH-SLTVSSTPSGQAN---VKAGKDQIT 60
           MA++ ++    L+ SL  S +G     +F EL   +L V++ PS +     + AGKD + 
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWALNRSISGVDTSIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQFAIVKRPYTS 120
           +TW L+ SI     + ++ ++ KLCY   SQ DRPWRKT +E+ +DKTC   I+ +PY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYDK 120

Query: 121 SSDSVTYTIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-VAGISGRSASI 180
           +  S T+T+++D+PT  YF+R Y  D  G + +AYGQ+T      +LF V  ISGR AS+
Sbjct: 121 TLQSTTWTLERDIPTGTYFVRAYAVDAIGHE-VAYGQSTDDAKKTNLFSVQAISGRHASL 180

Query: 181 DIAASIFSAFSVISLGFFFYLEKKKSK 195
           DIA+  FS FSV++L  FF  EK+K+K
Sbjct: 181 DIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Spo07639.1 vs. TAIR (Arabidopsis)
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein)

HSP 1 Score: 130.2 bits (326), Expect = 1.400e-30
Identity = 70/157 (44.59%), Postives = 103/157 (65.61%), Query Frame = 1


		  
Query: 44  VKAGKDQITVTWALNRSISGVDT-SIYREVEAKLCYHLESQKDRPWRKTEEEMARDKTCQ 103
           ++AGKD +T+TW L  S + VDT + ++ ++ KLCY   SQ DRPWRKT+ ++ +D++C 
Sbjct: 283 LEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCP 342

Query: 104 FAIVKRPYTSSSDSVTYTIKKDVPTAHYFIRVYVRDGPGGKLIAYGQTTG----LDLF-V 163
             IV + Y  +  S+ +TI  D+PT  YF+R Y  DG G + +AYGQ+T      +LF V
Sbjct: 343 HEIVSKAYDKTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHE-VAYGQSTDEGRTTNLFSV 402

Query: 164 AGISGRSASIDIAASIFSAFSVISLGFFFYLEKKKSK 195
             ISG    +DIA++ FS FSV+SL  FF +EK+K+K
Sbjct: 403 HAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 438

The following BLAST results are available for this feature:

BLAST of Spo07639.1 vs. NCBI nr
BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
BLAST of Spo07639.1 vs. ExPASy Swiss-Prot
BLAST of Spo07639.1 vs. TAIR (Arabidopsis)

BLAST of Spo07639.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902212412\|gb\|KNA16950.1\|	1.3e-102	100.	hypothetical protein SOVF_0842...	[more]
gi\|731376224\|ref\|XP_010667500.1\|	9.8e-66	64.1	PREDICTED: high-affinity nitra...	[more]
gi\|147825149\|emb\|CAN71077.1\|	7.8e-39	49.2	hypothetical protein VITISV_02...	[more]
gi\|225456868\|ref\|XP_002279861.1\|	1.0e-38	48.7	PREDICTED: high-affinity nitra...	[more]
gi\|661883623\|emb\|CDP12708.1\|	1.0e-38	47.2	unnamed protein product [Coffe...	[more]

BLAST of Spo07639.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RBT8_SPIOL	9.1e-103	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8B2J0_BETVU	6.8e-66	64.1	Uncharacterized protein OS=Bet...	[more]
A5CB21_VITVI	5.4e-39	49.2	Putative uncharacterized prote...	[more]
A0A068UYM9_COFCA	7.1e-39	47.2	Uncharacterized protein OS=Cof...	[more]
A0A124SGG2_CYNCS	7.1e-39	48.9	Uncharacterized protein OS=Cyn...	[more]

BLAST of Spo07639.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4


Match Name	E-value	Identity	Description
NRT32_ARATH	2.7e-31	40.9	High-affinity nitrate transpor...	[more]
NAR21_ORYSJ	1.0e-30	38.8	High-affinity nitrate transpor...	[more]
NRT31_ARATH	1.0e-30	41.0	High-affinity nitrate transpor...	[more]
NAR22_ORYSJ	5.1e-30	40.5	Probable high-affinity nitrate...	[more]

BLAST of Spo07639.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G50200.3	5.7e-32	41.0	nitrate transmembrane transpor...	[more]
AT4G24730.1	1.4e-30	44.5	Calcineurin-like metallo-phosp...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIR	PIRSF012939	NAR2	coord: 1..197 score: 3.8
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 25..192 score: 9.4
None	No IPR available	PANTHER	PTHR34806	FAMILY NOT NAMED	coord: 1..197 score: 1.1
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.1-RELATED	coord: 1..197 score: 1.1
None	No IPR available	PROFILE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..20 score:

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006865	amino acid transport
biological_process	GO:0009987	cellular process
biological_process	GO:0015706	nitrate transport
biological_process	GO:0050789	regulation of biological process
biological_process	GO:0010167	response to nitrate
biological_process	GO:0006950	response to stress
cellular_component	GO:0016020	membrane

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo13051	0.67	Barchart \| Table
Spo22604	0.67	Barchart \| Table
Spo19138	0.66	Barchart \| Table
Spo23823	0.66	Barchart \| Table